CN101084962A - Adsorption clarification method in preparing notoginseng total saponins - Google Patents
Adsorption clarification method in preparing notoginseng total saponins Download PDFInfo
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- CN101084962A CN101084962A CN 200710065967 CN200710065967A CN101084962A CN 101084962 A CN101084962 A CN 101084962A CN 200710065967 CN200710065967 CN 200710065967 CN 200710065967 A CN200710065967 A CN 200710065967A CN 101084962 A CN101084962 A CN 101084962A
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Abstract
The invention relates to a method of adsorption and clarification in the preparation of panax notoginseng saponins. The method comprises cutting radix notoginseng, pulverizing, reflux extracting under heating with 5-10 times of 75% ethanol in a multifunctional extraction tank for 3 times with 2 h for each time, combining the filtrate, concentrating under reduced pressue, adding ZTC clarifier 10-50% or 101 fruit juice clarifier 2-20% or chitosan clarifier 5-30%, standing for 3-12h, clarifying at 60-80deg.C, filtering, loading on macroporous resin column, eluting with 20-30 times the column volume of distilled water to obtain saccharide part, eluting with 4-6 times the column volume of 75-95% ethanol to obtain panax notoginseng saponins, separating with decolouring column made of ion exchange resin, concentrating, drying at 75deg.C under reduced pressure, pulverizing, and mixing to obtain panax notoginseng saponins. The inventive method of adsorption and clarification in the preparation of panax notoginseng saponins substitutes n-butanol extraction in original technology; has high efficiency, short production period, and low cost; can mostly reserve effective ingredient, improve stability and clarity; has no pollution; and overcomes the shortness in present technology.
Description
Technical field
The present invention relates to field of pharmaceutical technology, the adsorption clarification method in the Radix Notoginseng total arasaponins preparation specifically.
Background technology
Radix Notoginseng is the dry root of araliaceae ginseng plant Radix Notoginseng Panax notoginseng (Burk.) F.H.Chen, is Chinese distinctive rare Chinese medicine.Main product have the title of " treating blood disorders panacea " in the Yunnan mountain of papers, and it is again a kind of medicinal and edible plant simultaneously, can stop blooding subduing swelling and relieving pain by dissipating blood stasis.Be used for spitting of blood, spit blood, epistaxis is had blood in stool, metrorrhagia, and traumatic hemorrhage, the breast abdomen twinges, tumbling and swelling.Radix Notoginseng total arasaponins is its main active, modern pharmacology experiment showed, that the total saponins in the Radix Notoginseng can increase blood flow volume, blood vessel dilating, reduction arteriotony and myocardial oxygen consumption, improve body function and to anoxybiotic tolerance, and can anticoagulant, reduce blood viscosity etc.Has the wide development prospect in fields such as medicine, health product, functional food, cosmetics.To the many traditionally methods with the alcohol extracting-water precipitating n-butanol extraction of the extraction of saponin constituent in the Radix Notoginseng, this method is used organic solvents such as n-butyl alcohol, and toxicity is big, operates loaded down with trivial detailsly, and yield is low, the cost height.
The adsorption clarification technology is a kind of preparation technique of utilization absorptive clarificant with the solid-liquid sharp separation.The adsorption clarification technology is the treatment process that is used for sewage the earliest.Obtain general applying in fields such as fruit juice, beverage, wine, syrups afterwards.Be used for the clarification of Chinese medicine aqueous extract in recent years, be based on precipitating in clarifier and the Chinese medicine extraction liquid than thick microgranule generation copolymerization.It optionally with solution in colloid, protein, tannin complexation and coprecipitated, reach refining and improve a new technique of Chinese medicine quality through filtration.What adsorption clarification technology application at present was more is the clarification process of Chinese medicine oral liquid and injection.
Publication number is in the patent documentation of CN 1334267A disclosed " preparation method of Radix Notoginseng total arasaponins ", is to replace n-butanol extraction with resin column chromatography Solid-Phase Extraction, does not relate to adsorption clarification method.
Summary of the invention
The purpose of this invention is to provide a kind of have the efficient height, with short production cycle, cost is low, can keep morely effective ingredient, improve the adsorption clarification method in stability and clarity, the preparation of free of contamination Radix Notoginseng total arasaponins.
The present invention is achieved by following technical proposals:
After the pulverizing of Radix Notoginseng clip, 75% alcohol heating reflux of doubly measuring with 5-10 in multi-function extractor extracts 3 times, each 2 hours, merging filtrate, concentrating under reduced pressure, add ZTC clarifier 10-50% or 101 fruit juice clarifier 2-20% or chitosan 5%-30%, static 3-12 hour, clarifying temp 60-80 ℃, filter, filtrate adds macroporous resin column, with 20~30 times of column volume distilled water eluting saccharide parts, again with the 75-95% ethanol elution Radix Notoginseng total arasaponins of 4~6 times of column volumes, the decolorizing column that ethanol elution is formed by ion exchange resin, after eluent concentrated, drying under reduced pressure under 75 ℃ of conditions was pulverized, mix and obtain Radix Notoginseng total arasaponins.
Below be the experiment situation relevant with the present invention:
The kind of clarifier and selection: adopt 3 kinds of natural clarifying agents, ZTC clarifier or 101 fruit juice clarifiers or chitosan.The ZTC clarifier comprises 1+1, I, II, III, IV type.
The clarification process of different clarifiers adopts orthonormal design of experiments respectively 3 kinds of clarifiers to be carried out the screening of optimised process.
The result is compared as follows table 1
Sequence number | Clarifier | The retention rate of effective ingredient (%) | Solid content yield (%) | |||
Total saponins | Rg1 | Rb1 | R1 | |||
1 | Chitosan | 93.7 | 92.9 | 94.5 | 95.6 | 21.7 |
2 | 101 fruit juice clarifiers | 95.5 | 93.2 | 95.1 | 94.3 | 20.5 |
3 | The ZTC clarifier | 94.8 | 93.4 | 94.8 | 95.0 | 19.4 |
4 | Water precipitating | 90.3 | 91.7 | 92.6 | 90.1 | 20.3 |
The assay index composition that State Bureau issues Radix Notoginseng total arasaponins in the standard is total saponins, ginsenoside Rg1, ginsenoside Rb1, ginsenoside R1, so with these several index components content detection index that is the effective ingredient retention rate.The effect of representing to remove deproteinize, tannin etc. with the solid content yield.
By table 1 as seen, three kinds of clarifiers are to be higher than the extracting solution that water precipitating was handled to the retention rate of total saponins, ginsenoside Rg1, ginsenoside Rb1, ginsenoside R1, and the yield difference of solid content is very little, and the clarifying effect of three kinds of clarifiers is all more satisfactory.Illustrate and adopt clarifier both can reduce the yield of solid content than the water precipitation method, kept effective ingredient simultaneously again, use clarifier that technological process is shortened, production cost reduces, and has certain superiority.
Adsorption clarification technology of the present invention and n-butanol extraction technology are relatively
Respectively 5 batches of Radix Notoginseng have been carried out middle trial production with method of the present invention and the old technology of n-butanol extraction, the every crowd of 50kg that feeds intake.The color of test products, yield, saponin content, principal monomer saponin content, clarity.The results are shown in following table 2,3,4.
Table 2 adsorption clarification handicraft product statistic of attribute
Batch number | Major product performance rating index | |||||||
Color | Yield % | Clarity | Rb 1≥30% | Rg 1≥20% | R 1≥5% | ∑≥60% | Saponin content | |
20060201 | White | 15.1 | Qualified | 35.00 | 34.05 | 7.73 | 76.78 | 98.8 |
20060202 | White | 13.5 | Qualified | 33.00 | 32.02 | 7.80 | 72.82 | 97.6 |
20060203 | White | 14.2 | Qualified | 32.70 | 33.04 | 8.20 | 73.94 | 98.5 |
20060301 | White | 16.0 | Qualified | 34.02 | 37.08 | 7.60 | 72.70 | 99.1 |
20060302 | White | 13.4 | Qualified | 33.40 | 34.00 | 8.70 | 76.10 | 97.9 |
Subtotal | 72.2 | 168.12 | 170.19 | 40.03 | 372.34 | 491.9 | ||
Meansigma methods | 14.4 | 33.62 | 34.04 | 8.01 | 74.47 | 98.38 |
Table 3 n-butanol extraction handicraft product statistic of attribute
Batch number | Major product performance rating index | |||||||
Color | Yield % | Clarity | Rh 1≥30% | Rg 1≥20% | R 1≥5% | ∑≥60% | Saponin content | |
20060201 | Pale yellow | 10.2 | Qualified | 31.60 | 31.20 | 6.20 | 70.00 | 96.5 |
20060202 | Pale yellow | 9.8 | Qualified | 30.40 | 32.90 | 6.30 | 70.60 | 95.7 |
20060203 | Pale yellow | 11.6 | Qualified | 30.70 | 32.10 | 6.50 | 70.30 | 95.1 |
20060301 | Pale yellow | 12.7 | Qualified | 33.80 | 31.20 | 6.60 | 72.60 | 97.3 |
20060302 | Pale yellow | 11.4 | Qualified | 30.50 | 33.00 | 6.50 | 71.00 | 94.4 |
Subtotal | 55.7 | 157.00 | 160.4 | 32.1 | 254.5 | 479.0 | ||
Meansigma methods | 11.1 | 31.40 | 32.08 | 6.42 | 70.9 | 95.8 |
Table 4
The result shows: every index of the product of adsorption clarification explained hereafter all is higher than the product of n-butanol extraction explained hereafter.Adsorption clarification technology has improved yield and every main quality index of Radix Notoginseng total arasaponins significantly, and process cycle shortens 1/3rd, reduces cost, and has tangible economic benefit.
Because n-butyl alcohol no longer with an organic solvent, reducing Radix Notoginseng total arasaponins has the residual of agent solvent, reduces blowdown, and environmental conservation is had realistic meanings.
The used natural clarifying agent of the present invention is the additive made from natural material, safety non-toxic, and the clarification object is protein, waxiness, tannin etc., is the ideal absorptive clarificant of Radix Notoginseng total arasaponins.
Adsorption clarification method in the Radix Notoginseng total arasaponins of the present invention preparation can obtain being fit to the new preparation technology and the technical parameter of Radix Notoginseng total arasaponins of the big production of industrialization.Replace the n-butanol extraction of former technology with adsorption clarification, have the efficient height, with short production cycle, cost is low, can keep effective ingredient morely, improves stability and clarity, free of contamination advantage, has overcome the deficiencies in the prior art.
The specific embodiment
Embodiment 1
After Radix Notoginseng clip 10kg pulverizes, 75% alcohol heating reflux with 5 times of amounts in multi-function extractor extracts 3 times, each 2 hours, merging filtrate, concentrating under reduced pressure, add the ZTC clarifier (concentrated liquid measure) 10% for preparing, static 3 hours, filter, filtrate adds macroporous resin column, with 30 times of column volume distilled water eluting saccharide parts, with 75% ethanol elution Radix Notoginseng total arasaponins of 6 times of column volumes, ethanol elution is by the decolorizing column of ion exchange resin composition again, and with 3 times of column volume 75% ethanol elutions, after eluent concentrated, drying under reduced pressure under 75 ℃ of conditions was pulverized, mix and obtain Radix Notoginseng total arasaponins.
Embodiment 2
After Radix Notoginseng clip 10kg pulverizes, 75% alcohol heating reflux with 6 times of amounts in multi-function extractor extracts 3 times, each 2 hours, merging filtrate, concentrating under reduced pressure, add the ZTC clarifier (concentrated liquid measure) 20% for preparing, static 7 hours, filter, filtrate adds macroporous resin column, with 30 times of column volume distilled water eluting saccharide parts, with 80% ethanol elution Radix Notoginseng total arasaponins of 6 times of column volumes, ethanol elution is by the decolorizing column of ion exchange resin composition again, and with 3 times of column volume 80% ethanol elutions, after eluent concentrated, drying under reduced pressure under 75 ℃ of conditions was pulverized, mix and obtain Radix Notoginseng total arasaponins.
Embodiment 3
After Radix Notoginseng clip 10kg pulverizes, 75% alcohol heating reflux with 8 times of amounts in multi-function extractor extracts 3 times, each 2 hours, merging filtrate, concentrating under reduced pressure, add the ZTC clarifier (concentrated liquid measure) 30% for preparing, static 7 hours, filter, filtrate adds macroporous resin column, with 30 times of column volume distilled water eluting saccharide parts, with 85% ethanol elution Radix Notoginseng total arasaponins of 6 times of column volumes, ethanol elution is by the decolorizing column of ion exchange resin composition again, and with 3 times of column volume 85% ethanol elutions, after eluent concentrated, drying under reduced pressure under 75 ℃ of conditions was pulverized, mix and obtain Radix Notoginseng total arasaponins.
Embodiment 4
After Radix Notoginseng clip 10kg pulverizes, 75% alcohol heating reflux with 10 times of amounts in multi-function extractor extracts 3 times, each 2 hours, merging filtrate, concentrating under reduced pressure, add the ZTC clarifier (concentrated liquid measure) 50% for preparing, static 12 hours, filter, filtrate adds macroporous resin column, with 30 times of column volume distilled water eluting saccharide parts, with 95% ethanol elution Radix Notoginseng total arasaponins of 6 times of column volumes, ethanol elution is by the decolorizing column of ion exchange resin composition again, and with 3 times of column volume 95% ethanol elutions, after eluent concentrated, drying under reduced pressure under 75 ℃ of conditions was pulverized, mix and obtain Radix Notoginseng total arasaponins.
Embodiment 5
After Radix Notoginseng clip 10kg pulverizes, 75% alcohol heating reflux with 5 times of amounts in multi-function extractor extracts 3 times, each 2 hours, merging filtrate, concentrating under reduced pressure, add 101 fruit juice clarifiers (concentrated liquid measure) 2% that prepare, static 3 hours, filter, filtrate adds macroporous resin column, with 30 times of column volume distilled water eluting saccharide parts, with 75% ethanol elution Radix Notoginseng total arasaponins of 6 times of column volumes, ethanol elution is by the decolorizing column of ion exchange resin composition again, and with 3 times of column volume 75% ethanol elutions, after eluent concentrated, drying under reduced pressure under 75 ℃ of conditions was pulverized, mix and obtain Radix Notoginseng total arasaponins.
Embodiment 6
After Radix Notoginseng clip 10kg pulverizes, 75% alcohol heating reflux with 6 times of amounts in multi-function extractor extracts 3 times, each 2 hours, merging filtrate, concentrating under reduced pressure, add 101 fruit juice clarifiers (concentrated liquid measure) 8% that prepare, static 7 hours, filter, filtrate adds macroporous resin column, with 30 times of column volume distilled water eluting saccharide parts, with 80% ethanol elution Radix Notoginseng total arasaponins of 6 times of column volumes, ethanol elution is by the decolorizing column of ion exchange resin composition again, and with 3 times of column volume 80% ethanol elutions, after eluent concentrated, drying under reduced pressure under 75 ℃ of conditions was pulverized, mix and obtain Radix Notoginseng total arasaponins.
Embodiment 7
After Radix Notoginseng clip 10kg pulverizes, 75% alcohol heating reflux with 8 times of amounts in multi-function extractor extracts 3 times, each 2 hours, merging filtrate, concentrating under reduced pressure, add 101 fruit juice clarifiers (concentrated liquid measure) 15% that prepare, static 7 hours, filter, filtrate adds macroporous resin column, with 30 times of column volume distilled water eluting saccharide parts, with 85% ethanol elution Radix Notoginseng total arasaponins of 6 times of column volumes, ethanol elution is by the decolorizing column of ion exchange resin composition again, and with 3 times of column volume 85% ethanol elutions, after eluent concentrated, drying under reduced pressure under 75 ℃ of conditions was pulverized, mix and obtain Radix Notoginseng total arasaponins.
Embodiment 8
After Radix Notoginseng clip 10kg pulverizes, 75% alcohol heating reflux with 10 times of amounts in multi-function extractor extracts 3 times, each 2 hours, merging filtrate, concentrating under reduced pressure, add 101 fruit juice clarifiers (concentrated liquid measure) 20% that prepare, static 12 hours, filter, filtrate adds macroporous resin column, with 30 times of column volume distilled water eluting saccharide parts, with 95% ethanol elution Radix Notoginseng total arasaponins of 6 times of column volumes, ethanol elution is by the decolorizing column of ion exchange resin composition again, and with 3 times of column volume 95% ethanol elutions, after eluent concentrated, drying under reduced pressure under 75 ℃ of conditions was pulverized, mix and obtain Radix Notoginseng total arasaponins.
Embodiment 9
After Radix Notoginseng clip 10kg pulverizes, 75% alcohol heating reflux with 5 times of amounts in multi-function extractor extracts 3 times, each 2 hours, merging filtrate, concentrating under reduced pressure, add the chitosan clarifier (concentrated liquid measure) 5% for preparing, static 3 hours, filter, filtrate adds macroporous resin column, with 30 times of column volume distilled water eluting saccharide parts, with 75% ethanol elution Radix Notoginseng total arasaponins of 6 times of column volumes, ethanol elution is by the decolorizing column of ion exchange resin composition again, and with 3 times of column volume 75% ethanol elutions, after eluent concentrated, drying under reduced pressure under 75 ℃ of conditions was pulverized, mix and obtain Radix Notoginseng total arasaponins.
Embodiment 10
After Radix Notoginseng clip 10kg pulverizes, 75% alcohol heating reflux with 6 times of amounts in multi-function extractor extracts 3 times, each 2 hours, merging filtrate, concentrating under reduced pressure, add the chitosan clarifier (concentrated liquid measure) 10% for preparing, static 7 hours, filter, filtrate adds macroporous resin column, with 30 times of column volume distilled water eluting saccharide parts, with 80% ethanol elution Radix Notoginseng total arasaponins of 6 times of column volumes, ethanol elution is by the decolorizing column of ion exchange resin composition again, and with 3 times of column volume 80% ethanol elutions, after eluent concentrated, drying under reduced pressure under 75 ℃ of conditions was pulverized, mix and obtain Radix Notoginseng total arasaponins.
Embodiment 11
After Radix Notoginseng clip 10kg pulverizes, 75% alcohol heating reflux with 8 times of amounts in multi-function extractor extracts 3 times, each 2 hours, merging filtrate, concentrating under reduced pressure, add the chitosan clarifier (concentrated liquid measure) 20% for preparing, static 7 hours, filter, filtrate adds macroporous resin column, with 30 times of column volume distilled water eluting saccharide parts, with 85% ethanol elution Radix Notoginseng total arasaponins of 6 times of column volumes, ethanol elution is by the decolorizing column of ion exchange resin composition again, and with 3 times of column volume 85% ethanol elutions, after eluent concentrated, drying under reduced pressure under 75 ℃ of conditions was pulverized, mix and obtain Radix Notoginseng total arasaponins.
Embodiment 12
After Radix Notoginseng clip 10kg pulverizes, 75% alcohol heating reflux with 10 times of amounts in multi-function extractor extracts 3 times, each 2 hours, merging filtrate, concentrating under reduced pressure, add the chitosan clarifier (concentrated liquid measure) 30% for preparing, static 12 hours, filter, filtrate adds macroporous resin column, with 30 times of column volume distilled water eluting saccharide parts, with 95% ethanol elution Radix Notoginseng total arasaponins of 6 times of column volumes, ethanol elution is by the decolorizing column of ion exchange resin composition again, and with 3 times of column volume 95% ethanol elutions, after eluent concentrated, drying under reduced pressure under 75 ℃ of conditions was pulverized, mix and obtain Radix Notoginseng total arasaponins.
The preparation of clarifier:
1, the preparation of ZTC clarifier:
The A component: add water with the ZTC clarifier and stir into pasty state, and then add water, swelling 24h stirs, and is made into the viscose that contains ZTC clarifier 1%;
The B component: prepare 1% aqueous acetic acid earlier, get the ZTC clarifier then and add an amount of 1% aqueous acetic acid dissolving, stir into pasty state, add 1% aqueous acetic acid again, swelling 24h stirs, and is mixed with the viscose that contains ZTC clarifier 1%; Used by 1: 2 proportioning by A component and B component, during use, add the B component earlier, the back adds the A component.
2, the preparation of chitosan clarifier: the aqueous acetic acid with 1% is made into the clarifier of chitosan-containing clarifier 1%.
3, the preparation of 101 fruit juice clarifiers: water is made into the clarifier that contains 101 fruit juice clarifiers 5%.
Claims (4)
1, adsorption clarification method in a kind of Radix Notoginseng total arasaponins preparation, it is characterized in that: after the Radix Notoginseng clip is pulverized, 75% alcohol heating reflux of doubly measuring with 5-10 in multi-function extractor extracts 3 times, each 2 hours, merging filtrate, concentrating under reduced pressure, add ZTC clarifier 10-50% or 101 fruit juice clarifier 2-20% or chitosan clarifier 5%-30%, static 3-12 hour, clarifying temp 60-80 ℃, to filter, filtrate adds macroporous resin column, with 20~30 times of column volume distilled water eluting saccharide parts, with the 75-95% ethanol elution Radix Notoginseng total arasaponins of 4~6 times of column volumes, the decolorizing column that ethanol elution is formed by ion exchange resin is after eluent concentrates again, drying under reduced pressure under 75 ℃ of conditions is pulverized, mix and obtain Radix Notoginseng total arasaponins.
2, the adsorption clarification method in the Radix Notoginseng total arasaponins preparation according to claim 1 is characterized in that the following preparation of described ZTC clarifier process:
The A component: add water with the ZTC clarifier and stir into pasty state, and then add water, swelling 24h stirs, and is made into that to contain ZTC clear
The viscose of clear agent 1%;
B component: prepare 1% aqueous acetic acid earlier, get the ZTC clarifier then and add an amount of 1% aqueous acetic acid dissolving, and stir
Pasty state adds 1% aqueous acetic acid again, and swelling 24h stirs, and is mixed with the viscose that contains ZTC clarifier 1%;
Used by 1: 2 proportioning by A component and B component, during use, add the B component earlier, the back adds the A component.
3, the adsorption clarification method in the Radix Notoginseng total arasaponins preparation according to claim 1, it is characterized in that the following preparation of described chitosan clarifier process: the aqueous acetic acid with 1% is made into the clarifier of chitosan-containing clarifier 1%.
4, the adsorption clarification method in the Radix Notoginseng total arasaponins preparation according to claim 1, it is characterized in that the following preparation of described 101 fruit juice clarifiers process: water is made into the clarifier that contains 101 fruit juice clarifiers 5%.
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CN101229207B (en) * | 2008-01-30 | 2011-02-16 | 南开大学 | Decoloring refined technology of notoginseng total saponin duolite method |
CN102240322A (en) * | 2011-07-01 | 2011-11-16 | 天圣制药集团股份有限公司 | Compound red sage root tablet and preparing process thereof |
CN103251665A (en) * | 2013-04-23 | 2013-08-21 | 苏州谷力生物科技有限公司 | Purifying process of crude product of caltrop total saponins |
CN103494889A (en) * | 2013-10-08 | 2014-01-08 | 黄荣 | Traditional Chinese medicine for treating chilblain and preparation method thereof |
CN109432151A (en) * | 2018-12-29 | 2019-03-08 | 金七药业股份有限公司 | Method for improving Radix Notoginseng under ground portion recovery rate |
CN109498666A (en) * | 2019-01-25 | 2019-03-22 | 江苏农牧科技职业学院 | A kind of impurity-removing method of response phase method optimization notoginseng haulm saponin extract solution |
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CN101229207B (en) * | 2008-01-30 | 2011-02-16 | 南开大学 | Decoloring refined technology of notoginseng total saponin duolite method |
CN102240322A (en) * | 2011-07-01 | 2011-11-16 | 天圣制药集团股份有限公司 | Compound red sage root tablet and preparing process thereof |
CN103251665A (en) * | 2013-04-23 | 2013-08-21 | 苏州谷力生物科技有限公司 | Purifying process of crude product of caltrop total saponins |
CN103251665B (en) * | 2013-04-23 | 2014-08-06 | 苏州谷力生物科技有限公司 | Purifying process of crude product of caltrop total saponins |
CN103494889A (en) * | 2013-10-08 | 2014-01-08 | 黄荣 | Traditional Chinese medicine for treating chilblain and preparation method thereof |
CN110974857A (en) * | 2018-12-03 | 2020-04-10 | 泰州医药城国科化物生物医药科技有限公司 | Refined panax notoginseng saponins and purification preparation method for extracting panax notoginseng saponins |
CN109432151A (en) * | 2018-12-29 | 2019-03-08 | 金七药业股份有限公司 | Method for improving Radix Notoginseng under ground portion recovery rate |
CN109498666A (en) * | 2019-01-25 | 2019-03-22 | 江苏农牧科技职业学院 | A kind of impurity-removing method of response phase method optimization notoginseng haulm saponin extract solution |
CN114988987A (en) * | 2022-06-29 | 2022-09-02 | 陕西华泰生物精细化工有限公司 | Extraction method of peach carin phenol |
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