CN101077408A - Oil of aromatic turmeric gel for ocular application and its production process - Google Patents
Oil of aromatic turmeric gel for ocular application and its production process Download PDFInfo
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Abstract
The present invention is one kind of ophthalmic zedoary oil gel and its preparation process, and belongs to the field of medicine technology. The ophthalmic zedoary oil gel is compounded with carbomer gel as supplementary material, and consists of zedoary oil, solubilizer, bacteriostatic agent, gel matrix and pH regulator. Its preparation process includes the steps of mixing and grinding while adding water, sterilizing and packing. Animal model experiment shows that the ophthalmic zedoary oil gel has certain healing effect on ceratitis caused by herpes simplex virus and adenovirus. The ophthalmic zedoary oil gel has clinical healing effect on viral ceratitis.
Description
Technical field:
The invention belongs to field of medicaments, particularly relate to a kind of gel for eye use.
Background technology:
Viral keratitis (Viral Keratoconjunctivitis) is one of important blinding oculopathy, and the course of disease is longer, relapse rate height, clinical treatment difficulty.
Summary of the invention:
The objective of the invention is to utilize carbomer gel to be made into oil of aromatic turmeric gel for ocular application and preparing process thereof for adjuvant.
Prescription of the present invention is: (unit is a weight portion)
Oleum Curcumae 0.03-5 solubilizing agent 0.1-15 antibacterial 0.01-5
Gel-type vehicle 0.01-35 PH regulator
Solubilizing agent of the present invention is meant a kind of in polysorbas20, polysorbate40, polysorbate60 or the Tween 80.
Antibacterial of the present invention is meant a kind of in methyl hydroxybenzoate, ethyl hydroxybenzoate, propylparaben, thimerosal, phenoxyethanol, bromo geramine or the geramine.
Gel-type vehicle of the present invention is meant carbomer 934, Acritamer 940, carbomer 980, a kind of in methylcellulose, hydroxy methocel, hydroxypropyl cellulose, hydroxypropyl emthylcellulose, sodium carboxymethyl cellulose, ethyl cellulose, polyvinylpyrrolidone, crospolyvinylpyrrolidone, polyvinyl alcohol, the Polyethylene Glycol.
PH regulator of the present invention adopts triethanolamine or sodium hydroxide.
Preparation method of the present invention is at first Oleum Curcumae and solubilizing agent to be ground in mortar evenly, add antibacterial and be ground to dissolving fully, add propylene glycol and continue to be ground to evenly, add entry gradually, the limit edged is ground to mix homogeneously, and above-mentioned mixed liquor is transferred in the agitator;
The preparation gel: gel-type vehicle is added in the above-mentioned solution, 600 rev/mins be stirred to fully dissolving after, drip the PH regulator, make it to become even gel, continue to stir and also add water sterilization, fill.
Best compound method of the present invention is: Oleum Curcumae and Tween 80 are ground in mortar evenly, add ethyl hydroxybenzoate and be ground to dissolving fully, add propylene glycol and continue to be ground to evenly, add entry gradually, the limit edged is ground to mix homogeneously, and above-mentioned mixed liquor is transferred in the agitator;
The preparation gel: the recipe quantity carbomer 934 is added in the above-mentioned solution, 600 rev/mins be stirred to fully dissolving after, drip triethanolamine, make it to become even gel, continue to stir and also add water sterilization, fill.
The present invention utilizes carbomer gel to be made into oil of aromatic turmeric gel for ocular application for adjuvant, be applied to rabbit herpes simplex virus and adenovirus keratitis animal model, and its therapeutic effect made an appraisal, the result shows that oil of aromatic turmeric gel for ocular application all has certain curative effect to herpes simplex virus and adenovirus keratitis animal model.The rabbit that human simple herpesvirus and adenovirus are caused is tested viral cornea all obvious curative effects, and keratopathy degree and virus control group be P＜0.05 relatively, TCID
50Difference is greater than 1, and prompting: oil of aromatic turmeric gel for ocular application has the clinical treatment effect to viral keratitis.
The specific embodiment:
Prescription of the present invention is: (unit is a weight portion)
Oleum Curcumae 0.03-5 solubilizing agent 0.1-15 antibacterial 0.01-5
Gel-type vehicle 0.01-35 PH regulator
Solubilizing agent of the present invention is meant a kind of in polysorbas20, polysorbate40, polysorbate60 or the Tween 80.
Antibacterial of the present invention is meant a kind of in methyl hydroxybenzoate, ethyl hydroxybenzoate, propylparaben, thimerosal, phenoxyethanol, bromo geramine or the geramine.
Gel-type vehicle of the present invention is meant carbomer 934, Acritamer 940, carbomer 980, a kind of in methylcellulose, hydroxy methocel, hydroxypropyl cellulose, hydroxypropyl emthylcellulose, sodium carboxymethyl cellulose, ethyl cellulose, polyvinylpyrrolidone, crospolyvinylpyrrolidone, polyvinyl alcohol, the Polyethylene Glycol.
PH regulator of the present invention adopts triethanolamine or sodium hydroxide.
Preparation method of the present invention is at first Oleum Curcumae and solubilizing agent to be ground in mortar evenly, add antibacterial and be ground to dissolving fully, add propylene glycol and continue to be ground to evenly, add entry gradually, the limit edged is ground to mix homogeneously, and above-mentioned mixed liquor is transferred in the agitator;
The preparation gel: gel-type vehicle is added in the above-mentioned solution, 600 rev/mins be stirred to fully dissolving after, drip the PH regulator, make it to become even gel, continue to stir and also add water sterilization, fill.
Best compound method of the present invention is: Oleum Curcumae and Tween 80 are ground in mortar evenly, add ethyl hydroxybenzoate and be ground to dissolving fully, add propylene glycol and continue to be ground to evenly, add entry gradually, the limit edged is ground to mix homogeneously, and above-mentioned mixed liquor is transferred in the agitator;
The preparation gel: the recipe quantity carbomer 934 is added in the above-mentioned solution, 600 rev/mins be stirred to fully dissolving after, drip triethanolamine, make it to become even gel, continue to stir and also add water sterilization, fill.
Embodiment 1:
The 13g Oleum Curcumae with after the 20g polysorbas20 mixes, is mixed with 28g hydroxypropyl emthylcellulose and 500ml distilled water, and dispersed with stirring is even, forms thick glue.Getting the water-soluble back of 5g methyl hydroxybenzoate adds in the thick glue.With triethanolamine pH is transferred to 8.5, adding distil water is to 1000g, and the back 115 ℃ of sterilizations 30 minutes that stir make oil of aromatic turmeric gel for ocular application.
Embodiment 2:
3g carbomer 973 is spread in the 500ml water for injection, is uniformly dispersed.Spend the night, make its natural colloidal sol, transfer pH to 6.9 with 10mol/L NaOH (about 3ml).Take by weighing the 1.0g Oleum Curcumae and add the polysorbate40 mix homogeneously, be dissolved in the 20ml water for injection, be stirred to dissolving fully, the 0.22um filtering with microporous membrane adds filtrate in the thick glue, is stirred to evenly.Take by weighing the 0.5g ethyl hydroxybenzoate in 200ml water for injection, heating in water bath is stirred to dissolving fully, adds in the glue, stirs.Add water for injection to 1000g, record pH6.9.The glue for preparing is sealed 121 ℃ of high pressure moist heat sterilization 10min with kraft paper.Behind the indoor ultra-vioket radiation 2h of aseptic experiment, make oil of aromatic turmeric gel for ocular application.
Embodiment 3:
15g carbomer 934,5.0g Oleum Curcumae, 10g polysorbate40 and 0.5g geramine are mixed with 500ml water for injection, be uniformly dispersed, form thick glue.After the aseptic filtration of 0.30um microporous filter membrane, transfer pH to 7 with 10%NaOH, add the injection water to 1000g, 120 ℃ of sterilization 20min make oil of aromatic turmeric gel for ocular application.
Embodiment 4:
5g sodium carboxymethyl cellulose, 5g polyvinylpyrrolidone (K17, K30 or K90) are dispersed in the water, form thick glue.50g Oleum Curcumae, 20g polysorbate60,5g propylparaben and 15g sodium chloride with 4ml 10%NaOH dissolving, are added in the thick glue then, stir, add water to 1000g, back sterilization packing stirs.
Embodiment 5:
5g Carbopol 941,12g hydroxypropyl emthylcellulose are dispersed in the water, form thick glue, 121 ℃ of high pressure moist heat sterilization 10min.50g Oleum Curcumae, 50g Tween 80,10g phenoxyethanol with 5ml 10%NaOH dissolving, after the aseptic filtration of 0.30um microporous filter membrane, are added in the thick glue, stir, add water to 1000g, the back packing stirs.
Embodiment 6:
100g polyvinyl alcohol and 90g Oleum Curcumae are mixed with the 500ml distilled water, and dispersed with stirring is even, forms thick glue.Getting the water-soluble back of 8g thimerosal adds in the thick glue.Transfer pH to 8.5, adding distil water is to 1000g, and the back 115 ℃ of sterilizations 30 minutes that stir make oil of aromatic turmeric gel for ocular application.
Embodiment 7:
20g polyvinyl alcohol, 20g cetomacrogol 1000 and 120g Oleum Curcumae are mixed with the 500ml distilled water, and dispersed with stirring is even, forms thick glue.Getting the water-soluble back of 12g propylparaben adds in the thick glue.Transfer pH to 7.5, adding distil water is to 1000g, and the back 115 ℃ of sterilizations 30 minutes that stir make the Oleum Curcumae eye gel eye medicine.
Concrete experimentation of the present invention:
Test objective: this test objective is by the animal body inner model, observes the therapeutic effect of oil of aromatic turmeric gel for ocular application to viral keratitis.
Be subjected to the reagent thing: oil of aromatic turmeric gel for ocular application (the Oleum Curcumae absolute content is 0.2%), faint yellow translucent gels, lot number: 041201, Changchun Botai Biotechnology Co., Ltd. provides.
Compound method:
1,2g Oleum Curcumae and 10g Tween 80 are ground in mortar evenly, the ethyl hydroxybenzoate that adds recipe quantity is ground to dissolving fully, adds the 20g propylene glycol and continues to be ground to evenly, adds the water of about 150g gradually, and the limit edged is ground to mix homogeneously.Above-mentioned mixed liquor is transferred in the agitator.
2, preparation gel: the recipe quantity carbomer 934 is added in the above-mentioned solution, 600 rev/mins be stirred to complete swelling after, drip triethanolamine, make it to become even gel, continue to stir and also add water to 1000g, sterilization, fill.
Positive control drug: aciclovir eye drop, specification are 8ml (8mg) * 20, Wuhan Wujing Medicine Co., Ltd, lot number: 05010301.Purchase in the Changchun pharmacy.
Instrument: use the gas incubator, NCPC03100-1, the U.S. more; The profound hypothermia refrigerator, SANYO, Japan; Double superclean bench, SW-CT-ZF type, Chinese Suzhou; Centrifugal precipitation mechanism, LXT-II type, Chinese Shanghai; Vacuum freeze drier, MRK, Japan; Binocular microscope, OLYMPUSBHF, Japan.Digital camera, Sony 828, Japan; Centrifuge, LDZ5-2 type autobalance centrifuge, Beijing Medical Centrifugal Machine Factory.Corneal trephine, Chinese Suzhou.YZ-5F type slit lamp, Chinese Chengdu.
Reagent: bupivacaine hydrochloride, Shanghai Hefeng Pharmaceutical Co., Ltd., product batch number 0306031.The IMDM cell culture fluid, GIBCO company, the U.S.; Newborn calf serum, Dalian biochemical reagents factory; Trypsin, SIGMA, the U.S..
Cell: African green monkey kidney cell (Vero cell) is provided by regenerative medicine Science Institute of Jilin University virus research chamber.
Laboratory animal: 42 of Japan large ear rabbits, male and female half and half, body weight 1.75 ± 0.25kg purchases the Experimental Animal Center in preclinical medicine institute of Jilin University, the quality certification number: (Ji) 2003-2001.
Strain: herpes simplex virus I-type (herpes simplex virus, HSV-I), adenovirus III type (AdenovirusIII, ad
3), provide by regenerative medicine Science Institute of Jilin University virus research chamber.
1 experiment is divided into groups and is set up rabbit viral keratitis model
Experimental group adopts 36 of Japan large ear rabbits, male and female half and half, and single cage is raised.Concrete grammar: eyes are with 1% bupivacaine mucomembranous surface anesthesia, anterior corneal surface is divided into four quadrants, with the middle part of each quadrant of 3mm corneal trephine wound, be deep to bowman's lamina, scrape off the corneal epithelium of trepan wound site with knife blade, then in conjunctival sac, splash into 10TCID respectively
50Herpes simplex virus I-type and each 50 μ l of adenovirus III type virus liquid
[7], both hands fingers is clamped palpebra inferior respectively, make it to close and and become certain distance with eyeball, rock eyelid gently, can avoid viral liquid to ooze out, infect 18 Japan large ear rabbits respectively, consecutive infection 3 days, isolation nursing.Every day, lagophthalmos pathological changes situation observed in record.
The viral keratitis animal model is divided into 6 groups at random, 6 every group, is respectively herpes simplex infections matched group (dripping excipient every day); Herpes simplex virus positive drug treatment matched group; Herpes simplex virus oil of aromatic turmeric gel for ocular application treatment group; Adenovirus infection matched group (dripping excipient every day); Adenovirus positive drug treatment matched group; Adenovirus oil of aromatic turmeric gel for ocular application treatment group is established 6 of normal control groups (dripping excipient every day) simultaneously.
The treatment experiment of 2 viral keratitis animal models
Herpes simplex infections group and adenovirus infection group occur the viral keratitis symptom respectively after infecting 4d and 5d for the first time.The laboratory animal of herpes simplex infection group in the first time virus inoculation classical symptom of viral keratitis appearred after 4 days---cornea is given birth to nebula, be that point-like or dendroid pathological changes appear in anterior corneal surface, also can be observed the hyperemia of iris large tracts of land, with new vessels, pupil edge is unintelligible, and ring has gray area and photophobia, shed tears the symptom of secretions increase.The symptom that cornea is given birth to nebula appears in adenovirus infection group laboratory animal after inoculating 5 days first, but light than herpes simplex infections treated animal corneal epithelium lesion degree, it is unintelligible pupil also to occur, iris hyperemia, and photophobia is shed tears, the many symptoms of secretions.Each infection group and viral infection group inner model is divided into three groups at random, i.e. Oleum Curcumae treatment group, positive drug treatment group, virus control group.
2.1 all beginning of the 5th day behind the first time virus inoculation conjunctival sac administrations of Oleum Curcumae treatment group in experimental procedure herpes simplex infections group and the adenovirus infection group and positive drug treatment group, every day 6 times, per 2 hours once, successive administration 10 days.The administration of virus control group also together.Writing down every is used for that the experimental rabbit daughter heavily changes and changes in every index of the following regulation of slit lamp observation.After 10 days every experiment lagophthalmos is taken pictures, live and to get cornea of both eyes and put 5ml and contain in the 2% calf serum IMDM culture fluid, homogenate, stock solution, 10 is disposed in 0.22 μ m miniature displacer degerming respectively
-1, 10
-2, 10
-3, 10
-4, 10
-5, 10
-6Virus liquid with 200,000/mlVero cell inoculation, 96 porocyte culture plates (0.1ml/ hole), removes culture fluid after 24 hours, adds above-mentioned viral liquid 0.2ml/ hole, adopts the CPE method to detect each hole virus titer, adopts the Reed-Muench method to calculate LgTCID
50 [8], be judged to effectively greater than 1 with difference with the virus control group.
2.2 standards of grading and statistical method are observed every day, comprehensive multiple symptom and in conjunction with the Trouzdale standard
[9](the pathological changes area is 1 minute less than 25% of cornea area; 26%～50% is 2 minutes; 51%～75% is 3 minutes; 76%～100% is 4 minutes), make this experiment corneal inflammation shape standards of grading (seeing Table 1), relatively carry out statistical procedures between utilization independent sample group.
3 experimental results:
3.1 criterion of therapeutical effect
Produce effects: symptomatology disappears or the cardinal symptom sign disappears curative effect rate 〉=60%; Effectively: the cardinal symptom sign disappears substantially, curative effect rate 〉=30%; Invalid: the each side symptom and sign does not have improvement, curative effect rate＜30%.Curative effect rate=(the preceding integration of integration/treatment before the integration treatment of 1-treatment back) * 100%.And final therapeutic effect (seen Table 2,3, Fig. 1) carry out statistical evaluation.
3.2 evaluation to the viral keratitis therapeutic effect
In the herpes simplex infections group, the obvious effective rate of Oleum Curcumae treatment group is 83.3%, and effective percentage is 100%, and positive drug treatment group obvious effective rate is consistent with the former, and effective percentage is 83.3%.In the adenovirus infection group, the obvious effective rate of Oleum Curcumae treatment group is 83.3%, and apparently higher than positive drug treatment group (50%), two groups of effective percentage are all 100%.Oleum Curcumae does not have big difference to the therapeutic effect and the positive drug of herpes simplex infections group, and adenovirus infection group therapeutic effect obviously is better than the positive drug matched group.See Table 7
Each treatment stage is carried out statistical evaluation, during herpes simplex infections group treatment 3d, the relatively more visible significant difference (p＜0.05) of Oleum Curcumae treatment group and virus control group, during treatment 6d, compare poling significant difference (p＜0.01) with the virus control group, during treatment 10d, whole laboratory animal corneal epithelium pathological changes transference cures (p＜0.001), and treatment 6d is organized in the positive drug treatment, compares p＜0.05 with the virus control group; The treatment of adenovirus infection group is in the time of 2 days, Oleum Curcumae treatment group is compared with the virus control group and significant difference (p＜0.05) also occurred, different is, relatively be utmost point significant difference p＜0.001 two groups of 6d, and during positive drug treatment group treatment 3d, more do not show significant difference with the virus control group, see Table 6.
3.3 oil of aromatic turmeric gel for ocular application calculates each experimental group lgTCID to the inhibitory action of virus breeding with the Reed-Muench method
50, and with two treatment groups in each infection group and viral infection group and virus control group relatively, difference shows that all greater than 1 oil of aromatic turmeric gel for ocular application all has certain breeding inhibitory action (the results are shown in Table 8,9) to herpes simplex virus I-type and adenovirus III type.
3.4 influence to the weight of animals rate of increase
The body weight gain rate of normal control group is 7.83%, is the highest in all experimental grouies.In herpes simplex infections group and the adenovirus infection group, the body weight gain rate of Oleum Curcumae treatment group is respectively 2.17% and 2.47%, the rate of increase (1.78% and 1.80%) that is higher than positive drug treatment group, and negative growth has all appearred in two papova matched group body weight, the results are shown in Table 4.By above inferred from input data weight loss and the reason that increasess slowly may be because the discomfort that virus control group eye symptom causes causes animal diet followed and the unusual result of activity.Oleum Curcumae treatment group still keeps very high body weight gain rate, has proved that also oil of aromatic turmeric gel for ocular application has good therapeutical effect to viral keratitis.
Experiment conclusion
Experimental result shows that oil of aromatic turmeric gel for ocular application has the obvious treatment effect to rabbit viral keratitis model, and its therapeutical effect is better than aciclovir eye drop treatment group, and prompting: oil of aromatic turmeric gel for ocular application can be used as the clinical practice medicine of viral keratitis.
Symptom classification of table 1. viral keratitis animal model and standards of grading
| Symptom | Concrete manifestation | Scoring |
| Iris hyperemia | No visible vessels iris edge reddens in the iris, in the iris in the no visible vessels iris visible vessels iris muddiness appears, visible vessels is more, and whole iris reddens | 0123 |
| Shed tears | The absent lacrimation phenomenon shed tears less shed tears more shed tears more than | 0123 |
| Cornea is given birth to nebula | Cornea does not have dendroid or point-like or strip punctate keratitis cornea and dendroid occurs, point-like, the area that the area of strip punctate keratitis the punctate keratitis pathological changes occurs at 1/3 following cornea of whole cornea area reaches the area that dendroid or point-like or strip punctate keratitis appear in 1/3 to 2/3 cornea and accounts for more than 2/3 of whole cornea | 0123 |
| Pupil is unintelligible | Occurring occurring between gray zone of transition pupil and the iris gray zone between very clear pupil of pupil boundary and the iris broadens and can't differentiate the pupil size | 0123 |
| Photophobia | Not having special symptom photophobia desire blink photophobia blink photophobia dare not open eyes | 0123 |
| Conjunctival congestion | Bulbar conjunctiva is pure white, and capillary injection on the smooth conjunctiva has new vessels. | 01 |
| Secretions is many | Do not have the visible secretions of unnecessary secretions many around the eyelid, as many as covers eyeball | 01 |
* symptom score is added up, 0-4 is divided into acerous film inflammation shape, and 5-7 divides slight keratitis, and 8-12 divides moderate keratitis, 13-16 severe keratitis
The therapeutic effect of the viral keratitis that table 2. oil of aromatic turmeric gel for ocular application causes herpes simplex virus I-type
| Group | Numbering | Iris hyperemia | Shed tears | Secretions is many | Conjunctiva fills ware | Pupil is unintelligible | Photophobia | Cornea is given birth to nebula | The symptom average score | The curative effect rate | Scoring |
| The Rhizoma Curcumae oilhole is with doubting the glue group | 1 2 3 4 5 6 | 2 1 1 1 1 1 | 1 0 1 0 1 0 | 1 1 1 1 1 1 | 1 1 1 1 1 1 | 1 0 0 0 0 0 | 0 0 0 0 0 0 | 0 0 0 0 0 0 | 3.83±1.17 | 33 73 55 76 64 70 | 6 3 4 3 4 3 |
| The acyclovir matched group | 1 2 3 4 5 6 | 1 2 1 0 1 1 | 0 1 1 0 1 1 | 1 1 1 1 0 0 | 1 1 1 1 1 1 | 0 1 1 1 0 0 | 0 1 0 0 0 0 | 0 1 0 0 0 0 | 4.33±1.96 | 70 27 44 77 70 70 | 3 8 5 3 3 3 |
| 1 2 3 4 | 2 2 3 2 | 2 1 2 1 | 1 1 1 1 | 1 1 1 1 | 2 1 2 1 | 2 2 3 1 | 2 2 2 1 | ＜0 ＜0 ＜0 ＜0 | 12 10 13 8 |
| 5 6 | 2 3 | 2 2 | 1 1 | 1 1 | 2 2 | 1 2 | 2 3 | ＜0 ＜0 | 11 14 |
Table 3. oil of aromatic turmeric gel for ocular application is to the therapeutic effect of the caused viral keratitis of adenovirus III type
| Group | Numbering | Iris hyperemia | Shed tears | Secretions is many | Conjunctival congestion | Pupil is unintelligible | Photophobia | Cornea is given birth to nebula | The symptom average score | Curative effect is killed | Scoring |
| The Rhizoma Curcumae oilhole is with doubting the glue group | 1 2 3 4 5 6 | 1 1 2 1 0 1 | 0 0 1 0 1 0 | 1 1 1 1 1 1 | 1 1 1 1 1 1 | 1 0 1 0 0 0 | 0 0 0 0 0 0 | 0 0 0 0 0 0 | 3.66±1.21 | 64 73 33 70 70 66 | 4 3 6 3 3 3 |
| The acyclovir matched group | 1 2 3 4 5 6 | 1 1 2 0 1 1 | 0 1 1 0 1 1 | 1 1 1 1 0 0 | 1 1 1 1 1 1 | 0 1 1 1 0 0 | 0 0 0 0 0 0 | 0 0 0 0 0 0 | 4.00±1.26 | 70 44 50 73 55 75 | 3 5 6 3 4 3 |
| The virus control group | 1 2 3 4 5 6 | 2 3 3 2 2 2 | 2 2 2 1 1 2 | 1 1 1 1 1 1 | 1 1 1 1 1 1 | 2 2 2 1 2 2 | 2 2 3 1 1 2 | 2 3 2 2 2 2 | 11.33±1.50 | ＜0 ＜0 ＜0 ＜0 ＜0 ＜0 | 12 12 13 9 10 12 |
Each laboratory animal group body weight change in table 4. therapeutic process
| Group | Numbering | Body weight (kg) | Group | Numbering | Body weight (kg) | Group | Numbering | Body weight (kg) | |||
| Before the administration | After the administration | Before the administration | After the administration | Before the administration | After the administration | ||||||
| Herpes simplex virus oil of aromatic turmeric gel for ocular application group | 1 | 1.85 | 1.9O | Herpes simplex virus acyclovir group | 1 | 1.75 | 1.80 | The herpes simplex virus matched group | 1 | 1.65 | 1.70 |
| 2 | 1.95 | 1.95 | 2 | 1.70 | 1.75 | 2 | 1.95 | 1.95 | |||
| 3 | 2.05 | 2.1O | 3 | 1.75 | 1.75 | 3 | 2.05 | 2.00 | |||
| 4 | 2.10 | 2.10 | 4 | 1.70 | 1.70 | 4 | 2.10 | 2.00 | |||
| 5 | 2.00 | 2.10 | 5 | 1.95 | 2.05 | 5 | 1.85 | 1.85 | |||
| 6 | 1.70 | 1.75 | 6 | 2.05 | 2.05 | 6 | 1.95 | 2.05 | |||
| Rate of increase | 2.17％ | Rate of increase | 1.78％ | Rate of increase | -0.081％ | ||||||
| Group | Numbering | Body weight (kg) | Group | Numbering | Body weight (kg) | Group | Numbering | Body weight (kg) | |||
| Before the administration | After the administration | Before the administration | After the administration | Before the administration | After the administration | ||||||
| Adenovirus oil of aromatic turmeric gel for ocular application group | 1 | 1.85 | 1.85 | Adenovirus acyclovir group | 1 | 1.75 | 1.80 | Adenovirus virus control group | 1 | 1.65 | 1.75 |
| 2 | 1.95 | 1.70 | 2 | 1.70 | 1.85 | 2 | 1.95 | 1.70 | |||
| 3 | 2.05 | 1.95 | 3 | 1.75 | 1.90 | 3 | 2.05 | 1.85 | |||
| 4 | 2.10 | 2.00 | 4 | 1.70 | 2.00 | 4 | 2.10 | 1.60 | |||
| 5 | 2.00 | 1.65 | 5 | 1.95 | 1.95 | 5 | 1.85 | 1.90 | |||
| 6 | 1.70 | 1.75 | 6 | 2.05 | 1.85 | 6 | 1.95 | 1.85 | |||
| Rate of increase | 2.47％ | Rate of increase | 1.80％ | Rate of increase | -0.44％ | ||||||
Table 5. is respectively organized symptom score (x ± S branch)
| Group | Before the treatment | 3d | 6d | 10d |
| Herpes simplex virus oil of aromatic turmeric gel for ocular application treatment group herpes simplex virus acyclovir treatment group herpes simplex virus matched group | 10.50±1.51 10.50±1.37 10.67±1.75 | 8.50 ± 1.04 9.17 scholars 0.98 10.67 ± 1.75 | 7.83±0.98 8.33±1.03 10.67±1.75 | 3.83±1.17 4.33±1.96 11.66±1.63 |
| Group | Before the treatment | 3d | 6d | 10d |
| Adenovirus oil of aromatic turmeric gel for ocular application pool treatment group adenovirus A Ganluowei treatment group adenovirus matched group CK group | 10.00 ± 0.89 9.67 scholar 1.75 10.83 ± 1.47 0 | 8.33±1.17 9.33±0.81 10.83±1.47 0 | 5.67±0.82 6.50±1.05 10.83±1.47 0 | 3.67±1.21 4.00±1.26 11.33±1.50 0 |
Comparison (the P value of table 6. oil of aromatic turmeric gel for ocular application effect of different treatment time
*)
| The medicine group | Before the treatment | 3d | 6d | 10d |
| Herpes simplex virus oil of aromatic turmeric gel for ocular application treatment group herpes simplex virus acyclovir treatment group adenovirus oil of aromatic turmeric gel for ocular application treatment group adenovirus acyclovir treatment group | P＞0.05 P＞0.05 P＞0.05 P＞0.05 | P＜0.05 P＞0.05 P＜0.05 P＞0.05 | P＜0.01 P＜0.05 P＜0.001 P＜0.001 | P＜0.001 P＜0.001 P＜0.001 P＜0.001 |
*Compare with the virus control group
Table 7. oil of aromatic turmeric gel for ocular application is to the therapeutic effect of rabbit viral keratitis
| Group | Total routine number | Produce effects (%) | Effectively (%) | Invalid (%) |
| Herpes simplex virus oil of aromatic turmeric gel for ocular application treatment group herpes simplex virus acyclovir treatment group adenovirus oil of aromatic turmeric gel for ocular application treatment group adenovirus acyclovir treatment group | 6 6 6 6 | 4(66.7) 4(66.7) 5(83.3) 3(50.0) | 6(100) 5(83.3) 6(100) 6(100) | 0(0) 1(16.7) 0(0) 0(0) |
Table 8. oil of aromatic turmeric gel for ocular application is to herpes simplex infections group TCID
50Influence
| Grouping | lgTCID 50 | Difference |
| Herpes simplex virus matched group herpes simplex virus oil of aromatic turmeric gel for ocular application treatment group herpes simplex virus acyclovir treatment group | 2.70 1.30 1.21 | 0 1.49 1.40 |
Table 9. oil of aromatic turmeric gel for ocular application is to adenovirus infection group TCID
50Influence
| Grouping | lgTCID 50 | Difference |
| Adenovirus matched group adenovirus oil of aromatic turmeric gel for ocular application treatment group adenovirus acyclovir treatment group | 3.22 1.20 1.10 | 0 2.02 2.12 |
Claims (7)
1, a kind of oil of aromatic turmeric gel for ocular application, its prescription is: (unit is a weight portion)
Oleum Curcumae 0.03-5 solubilizing agent 0.1-15 antibacterial 0.01-5
Gel-type vehicle 0.01-35 PH regulator.
2, oil of aromatic turmeric gel for ocular application according to claim 1 is characterized in that: solubilizing agent is meant a kind of in polysorbas20, polysorbate40, polysorbate60 or the Tween 80.
3, oil of aromatic turmeric gel for ocular application according to claim 1 is characterized in that: antibacterial is meant a kind of in methyl hydroxybenzoate, ethyl hydroxybenzoate, propylparaben, thimerosal, phenoxyethanol, bromo geramine or the geramine.
4, oil of aromatic turmeric gel for ocular application according to claim 1, it is characterized in that: gel-type vehicle is meant carbomer 934, Acritamer 940, carbomer 980, a kind of in methylcellulose, hydroxy methocel, hydroxypropyl cellulose, hydroxypropyl emthylcellulose, sodium carboxymethyl cellulose, ethyl cellulose, polyvinylpyrrolidone, crospolyvinylpyrrolidone, polyvinyl alcohol, the Polyethylene Glycol.
5, oil of aromatic turmeric gel for ocular application according to claim 1 is characterized in that: the PH regulator adopts triethanolamine or sodium hydroxide.
6, the preparation method of the described oil of aromatic turmeric gel for ocular application of claim 1, it is characterized in that: Oleum Curcumae and solubilizing agent are ground in mortar evenly, add antibacterial and be ground to dissolving fully, adding propylene glycol continues to be ground to evenly, add entry gradually, the limit edged is ground to mix homogeneously, and above-mentioned mixed liquor is transferred in the agitator;
The preparation gel: gel-type vehicle is added in the above-mentioned solution, 600 rev/mins be stirred to fully dissolving after, drip the PH regulator, make it to become even gel, continue to stir and also add water sterilization, fill.
7, the preparation method of oil of aromatic turmeric gel for ocular application according to claim 6, it is characterized in that: its best compound method is: Oleum Curcumae and Tween 80 are ground in mortar evenly, add ethyl hydroxybenzoate and be ground to dissolving fully, adding propylene glycol continues to be ground to evenly, add entry gradually, the limit edged is ground to mix homogeneously, and above-mentioned mixed liquor is transferred in the agitator;
The preparation gel: the recipe quantity carbomer 934 is added in the above-mentioned solution, 600 rev/mins be stirred to fully dissolving after, drip triethanolamine, make it to become even gel, continue to stir and also add water sterilization, fill.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN 200610016878 CN101077408A (en) | 2006-05-26 | 2006-05-26 | Oil of aromatic turmeric gel for ocular application and its production process |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 200610016878 CN101077408A (en) | 2006-05-26 | 2006-05-26 | Oil of aromatic turmeric gel for ocular application and its production process |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110212142A1 (en) * | 2008-11-17 | 2011-09-01 | Laila Pharmaceuticals Pvt.Ltd. | Curcuminoids and its metabolites for the application in ocular diseases |
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2006
- 2006-05-26 CN CN 200610016878 patent/CN101077408A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110212142A1 (en) * | 2008-11-17 | 2011-09-01 | Laila Pharmaceuticals Pvt.Ltd. | Curcuminoids and its metabolites for the application in ocular diseases |
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