CN101067005A - Process of producing cordycepic polysaccharide - Google Patents
Process of producing cordycepic polysaccharide Download PDFInfo
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- CN101067005A CN101067005A CNA2007100523574A CN200710052357A CN101067005A CN 101067005 A CN101067005 A CN 101067005A CN A2007100523574 A CNA2007100523574 A CN A2007100523574A CN 200710052357 A CN200710052357 A CN 200710052357A CN 101067005 A CN101067005 A CN 101067005A
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Abstract
The process of producing cordycepic polysaccharide with rice includes the steps of compounding material, inoculating, culturing, harvesting cordycepic polysaccharide, radiation to sterilize and packing. The material includes rice 45.0-49.0 wt%, mixed corn and bean cake powder 0.9-2 wt %, and nutrient solution 49.0-54.1 wt%; and the nutrient solution is compounded with potato, sugar, peptone, potassium dihydrogen phosphate, magnesium sulfate and water in certain weight proportion. The cordycepic polysaccharide is suitable for cancer patient and person in subhealth state to take.
Description
Technical field
The present invention relates to field of health care food, particularly a kind of method of producing Cordyceps polysaccharide with rice.
Background technology
Natural Cordyceps sinensis is the special product of China.Because its strict parasitics and special ecogeography environment, natural resource are very rare, add the destruction of ecotope in recent years, the Qinghai-Tibet Platean temperature rises, the Cordyceps sinensis resource is fallen sharply, and endangered edge can't satisfy health care and medicinal demand at all.
Summary of the invention
Technical problem to be solved by this invention is: a kind of method of producing Cordyceps polysaccharide with rice is provided, and this method technology is simple, and product cost is low, and has similar health care and medicinal effect can substitute natural cordyceps because of product.
The present invention adopts the method with rice production Cordyceps polysaccharide that may further comprise the steps:
A. prepare burden:
Each proportion of raw materials is: by weight, and rice 45.0%-49.0%, corn and soya-bean cake powder mix 0.9%～2.0%, 49.0%～54.1%.
Nutrient solution prescription is: potato 180～220g, white sugar 18～22g, peptone 13～18g, potassium primary phosphate 1.5～2.2g, sal epsom 08～1.2g, water 900～1100g.
B. inoculation: earlier with batch mixes, put into Cans, again Cans are put into the high-pressure sterilizing pot sterilization, after cooling, inoculation Cordyceps C963.
C. cultivate: under 20～25 ℃ of conditions, cultivated 45～60 days, grow Cordyceps mycelium.
D. Cordyceps mycelium is carried out the separation and Extraction of polysaccharide, drying is pulverized, and obtains Cordyceps polysaccharide.
E. with Cordyceps polysaccharide behind irradiation sterilization, can pack.
The present invention has following major advantage:
One. selection is correct: thick rice raw material sources are extensive, cheap, can reduce the production cost of Cordyceps polysaccharide significantly, also provide valid approach for the rice deep processing.
They are two years old. and can substitute natural cordyceps: the Cordyceps polysaccharide of producing has similar Chemical Composition, pharmacological action and clinical efficacy to natural Cordyceps polysaccharide; and cost is low; therefore can substitute natural cordyceps, thereby make contributions for the day by day rare natural resource Cordyceps sinensis of protection exempts from extinction.
They are three years old. and production method has science and novelty, can improve body immunity effectively with its Cordyceps polysaccharide of producing, cancer-resisting, real is patient's ideal protective foods.
They are three years old. and product safety: the Cordyceps polysaccharide of producing experiment showed, without any side effects through institute for drug control, Hubei Province animal toxicology.
They are four years old. and be beneficial to popularization: production technique is simple, and is free from environmental pollution.
Embodiment
The invention will be further described below in conjunction with specific embodiment, but do not limit the present invention.
One. produce the Cordyceps polysaccharide culture medium prescription with rice
Its component comprises: by weight, and rice 45.0～49.0g, corn and soya-bean cake powder mix 0.9～2g, nutritive medium 49.0～54.1g (seeing attached list 1).Rice 45-49, corn and soya-bean cake powder mix 0.9-2.0, nutritive medium 54-59.
Above-mentioned rice can be selected thick rice for use, cracks rice, grain in storage for years.
Above-mentioned corn and soya-bean cake powder mix, the ratio of its weight is 1: 1, crosses 20～30 mesh sieves, water content≤13%.
Two. the preparation of Cordyceps polysaccharide
May further comprise the steps:
A. select materials (seeing attached list 2):
Thick rice: free from insect pests, nothing is gone mouldy, water content≤14%.
Corn soya-bean cake (1: 1) powder mix: cross the 20-30 mesh sieve, water content≤13%; Nutrient solution prescription is: potato 200g, white sugar 20g, peptone 15g, potassium primary phosphate 2g, sal epsom 1g, 900～1100g.
B. prepare burden:
The weight percent of each raw material is: thick rice 45-49g, corn and soya-bean cake (1: 1) powder mix 0.9-1.6g, nutritive medium 54-59g (seeing attached list 1).
C. processing: after the batch mixes, carry out autoclaving, inoculate Cordyceps C963 after cooling.Under 20-25 ℃ of condition, cultivate and grew Cordyceps mycelium in 45-60 days.Adopt water-soluble → alcohol precipitation → centrifugation to carry out Cordyceps polysaccharide again and extract, make the thick Cordyceps polysaccharide of chocolate brown powder shape (content is 27.4%).Cordyceps species C963 is provided by Hubei Prov. Acdemy of Agricutural Sciences.
D. irradiation requires:
Dose limitation: Cordyceps polysaccharide cobalt-60 irradiation average absorption amount≤7Kgy, best irradiation sterilization dosage is 6KGy.
Irradiation requires: irradiation is even, and dosage is accurate, average absorbed dose inequality degree≤2.0.Product by above-mentioned explained hereafter goes out meets edible demand, and its test rating sees attached list 3.
Three. thick cadelle grass polysaccharide acute toxinology experiment
1. test materials:
1. animal: Kunming mouse.Body weight 20 ± 2g, male and female half and half are provided by Hubei Province's medical experiment animal center, animal credit number: SCXK (Hubei Province) 2003-0005
2. trial-product: thick cadelle grass polysaccharide: 20050517, specification: former powder (thick cadelle grass polysaccharide) is provided by the Hubei Province Academy of Agricultural Sciences, the processing of agricultural products and Nuclear Agricultural Research Institute.Face the suspension that is mixed with the concentration of 0.36g/ml (peak concentration) with 0.5%CMC-Na with preceding.
2. test method:
According to the result of trial test, get 20 of above-mentioned mouse, male and female half and half are pressed 0.4ml/10g volume gastric infusion, observe animal immediate reaction and continuous the observation 7 after the administration.Do not see that toxic reaction appearred in animal in 7 days after the administration, comprise the toxic reactions such as fur, activity, feed and death of animal.The result shows: thick cadelle grass polysaccharide is to not toxigenicity reaction of animal.
3. the result is under above-mentioned test conditions, and thick cadelle grass polysaccharide is not seen animal dead through gastric infusion of mouse, does not measure LD50, and its maximum dosage-feeding is 14.4g/kg.Press 10g/kg.Answer true border nontoxic.
Four. Cordyceps polysaccharide provided by the invention can be made into capsule or other formulation.
Five. subordinate list
Table 1 component (weight %)
Table 2 product physical and chemical index
| Project | Index |
| Moisture content, % | ≤10.0 |
| Ash content, % | ≤8.0 |
| Cordyceps polysaccharide, % | ≥20.0 |
| Arsenic (in As), mg/kg | ≤0.5 |
| Plumbous (in Pb), mg/kg | ≤1.0 |
| Mercury (in Hg), mg/kg | ≤0.3 |
| Phenyl-hexachloride, mg/kg | ≤0.2 |
| Dichlorodiphenyl trichloroethane, mg/kg | ≤0.2 |
Table 3 product microbiological indicator
| Project | Index |
| Total number of bacterial colony, cfu/g | ≤3000 |
| Coliform, MPN/100g | ≤90 |
| Mould, cfu/g | ≤50 |
| Pathogenic bacterium (duodenum 12 road pathogenic bacterium and curing the disease property coccus) | Must not detect |
Claims (3)
1. method of producing Cordyceps polysaccharide is characterized in that adopting the method for producing Cordyceps polysaccharide with rice that may further comprise the steps:
A. prepare burden:
Each proportion of raw materials is: by weight, rice 45.0%～49.0%, corn and soya-bean cake powder mix 0.9%～2%, nutritive medium 49.0%～54.1%,
Nutrient solution prescription is: potato 180～220g, white sugar 18～22g, peptone 13～18g, potassium primary phosphate 1.5～2.2g, sal epsom 0.8～1.2g, water 900～1100g;
B. inoculation: earlier with batch mixes, put into Cans, again Cans are put into the high-pressure sterilizing pot sterilization, after cooling, inoculation Cordyceps C963;
C. cultivate: under 20～25 ℃ of conditions, cultivated 45～60 days, grow Cordyceps mycelium;
D. Cordyceps mycelium is carried out the separation and Extraction of polysaccharide, drying is pulverized, and obtains Cordyceps polysaccharide;
E. with Cordyceps polysaccharide behind irradiation sterilization, can pack.
2. the method for production Cordyceps polysaccharide according to claim 1 is characterized in that described rice is thick rice, and its requirement is: free from insect pests, nothing is gone mouldy, water content≤14%.
3. the method for production Cordyceps polysaccharide according to claim 1 is characterized in that described corn and soya-bean cake powder mix, and the ratio of its weight is 1: 1, and its requirement is: cross 20～30 mesh sieves, water content≤13%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100523574A CN101067005A (en) | 2007-06-01 | 2007-06-01 | Process of producing cordycepic polysaccharide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100523574A CN101067005A (en) | 2007-06-01 | 2007-06-01 | Process of producing cordycepic polysaccharide |
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| Publication Number | Publication Date |
|---|---|
| CN101067005A true CN101067005A (en) | 2007-11-07 |
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| CNA2007100523574A Pending CN101067005A (en) | 2007-06-01 | 2007-06-01 | Process of producing cordycepic polysaccharide |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102604804A (en) * | 2012-03-29 | 2012-07-25 | 长阳金梅生物科技有限公司 | Cordyceps militaris wine and preparation method thereof |
| CN102618598A (en) * | 2012-04-16 | 2012-08-01 | 山东省农业科学院农业资源与环境研究所 | Liquid fermentation method for improving yield of cordyceps sinensis polysaccharide by utilizing expansin |
| CN103864945A (en) * | 2014-02-21 | 2014-06-18 | 湖北省农业科学院农产品加工与核农技术研究所 | Method for intensively extracting polysaccharides from rice culture medium for cordyceps militaris |
| CN105039452A (en) * | 2015-06-23 | 2015-11-11 | 中山鼎晟生物科技有限公司 | Method for extracting polysaccharides in solid fermentation broth of cordyceps sinensis |
-
2007
- 2007-06-01 CN CNA2007100523574A patent/CN101067005A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102604804A (en) * | 2012-03-29 | 2012-07-25 | 长阳金梅生物科技有限公司 | Cordyceps militaris wine and preparation method thereof |
| CN102604804B (en) * | 2012-03-29 | 2016-03-16 | 湖北仁仁生物科技有限公司 | A kind of cordyceps wine and preparation method thereof |
| CN102618598A (en) * | 2012-04-16 | 2012-08-01 | 山东省农业科学院农业资源与环境研究所 | Liquid fermentation method for improving yield of cordyceps sinensis polysaccharide by utilizing expansin |
| CN102618598B (en) * | 2012-04-16 | 2013-08-21 | 山东省农业科学院农业资源与环境研究所 | Liquid fermentation method for improving yield of cordyceps sinensis polysaccharide by utilizing expansin |
| CN103864945A (en) * | 2014-02-21 | 2014-06-18 | 湖北省农业科学院农产品加工与核农技术研究所 | Method for intensively extracting polysaccharides from rice culture medium for cordyceps militaris |
| CN103864945B (en) * | 2014-02-21 | 2016-01-20 | 湖北省农业科学院农产品加工与核农技术研究所 | A kind of method strengthening polysaccharide in extraction Cordyceps militaris rice medium |
| CN105039452A (en) * | 2015-06-23 | 2015-11-11 | 中山鼎晟生物科技有限公司 | Method for extracting polysaccharides in solid fermentation broth of cordyceps sinensis |
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