CN101036702A - Supercritical CO2 liquid method for extracting efficient element of golden buckwheat - Google Patents
Supercritical CO2 liquid method for extracting efficient element of golden buckwheat Download PDFInfo
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Abstract
The invention relates to a process for separating active ingredient of Chinese herbal medicine wild buckwheat rhizome by supercritical CO2 fluid extraction, which belongs to chemistry and chemical engineering technology art for preparing Chinese herbal medicine. The characteristic of the invention is in that: nonpolar invalid components are extracted from the Chinese herbal medicine wild buckwheat rhizome by the supercritical CO2 fluid; polar invalid components are extracted from the Chinese herbal medicine wild buckwheat rhizome by an active solvent of absolute ethyl alcohol as an entrainer; the supercritical fluid extract has two stages of desorption processes, and the final extract remaining in an extraction kettle is active ingredient of the wild buckwheat rhizome in which the inactive components has already been removed. The extract remains are further extracted to obtain the active ingredient extract. The wild buckwheat rhizome extract of the invention has higher activity for inhibiting alpha-glucosaccharase and is applicable for a hypoglycemic drug. The invention has simple process, convenient operation, without harmful solvent remains in the product.
Description
Technical field
The present invention relates to a kind of supercritical CO
2Fluid extraction separates the process of Chinese herbal medicine wild buckwheat rhizome effective ingredient, belongs to Chinese herbal medicine and prepares technical field of chemistry and chemical engineering.
Background technology
Rhizoma Fagopyri Dibotryis Fagopyrum dibotrys (D.Don) Hara. is the root of buckwheat plant, have another name called Herba Polygoni cymosi, Jinsuoyinkai ', day Semen Fagopyri Esculenti, Semen Fagopyri Esculenti Radix Notoginseng etc. originate in ground such as Shaanxi, Henan, Jiangsu, Zhejiang, Jiangxi, Hubei, Guangdong, Guangxi, Guizhou, Yunnan, Sichuan.Excavate autumn, dries, and gives birth to usefulness, also with bright product, property acid, hardship, cold.Rhizoma Fagopyri Dibotryis has heat-clearing and toxic substances removing, reducing swelling and alleviating pain, the function of restraining growth of cancer cells, can be used for the treatment of lung abscess, pulmonary tuberculosis, emphysema, bronchiectasis, chronic bronchitis, tropical eosinophilia, arthralgia, dyspepsia etc., and wild Rhizoma Fagopyri Dibotryis has higher drug value.
The Chinese medicine extracting method generally needs further to handle to the extract of Chinese herbal medicine, and organic solvent is easily residual, and processing step is loaded down with trivial details, and shortcomings such as length expend time in.In recent years, fast development along with supercritical liquid extraction technique, and have and be fit to extract natural heat-sensitive substance, product does not have residual, the steady quality of hazardous solvent, easy to operate, extraction efficiency is high and good characteristics such as less energy consumption, for modernization of cmm provides a kind of convenient means, obtained wide application.At present for the Rhizoma Fagopyri Dibotryis supercritical CO
2The research of fluid extraction someone is as yet reported.
Summary of the invention
The purpose of this invention is to provide a kind of supercritical CO that passes through
2The process of fluid extraction Chinese herbal medicine wild buckwheat rhizome effective ingredient.Another object of the present invention is by this process, can at utmost reduce the influence of non-active ingredient in the later stage separation and Extraction, obtains to have higher alpha-glucosidase and suppress active effective ingredient.
Supercritical CO of the present invention
2The extraction process of fluid extraction Chinese herbal medicine wild buckwheat rhizome effective ingredient mainly adopts two kinds of methods:
1, at first uses supercritical CO
2Fluid carries out the extraction of nonpolar invalid components in the Rhizoma Fagopyri Dibotryis, does the extraction that entrainer carries out Rhizoma Fagopyri Dibotryis Semi-polarity invalid components again with organic solvent then; To do further to extract to handle through the Rhizoma Fagopyri Dibotryis raffinate after the extraction at last, obtain effective component extracts.
2, use supercritical CO
2Fluid and entrainer organic solvent be the extraction of the nonpolar invalid components of Rhizoma Fagopyri Dibotryis and polar invalid components simultaneously; To do further to extract to handle through the Rhizoma Fagopyri Dibotryis raffinate after the extraction at last, obtain effective component extracts.
Now above-mentioned two kinds of methods are described in the back:
1, the present invention is a kind of method of supercritical carbon dioxide extraction efficient element of golden buckwheat, it is characterized in that having following technical process and step:
A. phase I: use supercritical CO
2Nonpolar invalid components in the fluid extraction Rhizoma Fagopyri Dibotryis; With supercritical CO
2Fluid is a solvent, in extraction kettle Rhizoma Fagopyri Dibotryis is extracted; The particulate granularity of Rhizoma Fagopyri Dibotryis is 28～60 orders; Extracting pressure is 10～30MPa, and extraction temperature is 35～60 ℃, CO
2Flow is 25～40Kg/h, and the extraction time is 1～3 hour; Extract is at CO
2Fluid enters first separating still under driving, and carries out the first order and resolves, and parsing pressure is 7～8MPa, and resolution temperature is 50～55 ℃; Not resolved extract in the first order is resolved is at CO
2Under the fluidic drive, enter second separating still, carry out the second level and resolve, parsing pressure is 5～6MPa, and resolution temperature is 35～40 ℃; The extract of resolving gained is nonpolar invalid components;
B. second stage: on the phase I basis, add organic solvent, further extract polar invalid components as entrainer; The raffinate retained in the extraction kettle in phase I as extraction feed, is made entrainer with the organic solvent dehydrated alcohol, described raffinate is carried out supercritical CO
2The extraction of fluidic polar invalid components; Extracting pressure is 15～35MPa, and temperature is 35～55 ℃, CO
2Flow is 25～40Kg/h, and the extraction time is 1～3 hour; The flow of entrainer organic solvent dehydrated alcohol is 1.5～3.0L/h; Then, with supercritical CO
2Fluid extract carries out resolving twice by aforementioned the same manner; Be CO
2The fluid band the extract gradation and is entered separating still; The parsing pressure that the first order is resolved is 7～8MPa, and resolution temperature is 50～55 ℃; The parsing pressure that resolve the second level is 5～6MPa, and resolution temperature is 35～40 ℃; The extract of resolving gained is polar invalid components;
C. the further extraction of the Rhizoma Fagopyri Dibotryis raffinate behind supercritical extraction is handled: extract to handle and take dual mode, obtain extract A and extract B respectively, two kinds of extracting modes are:
(1) the Rhizoma Fagopyri Dibotryis raffinate after supercritical extraction is again through dehydrated alcohol extraction, and the gained material is an extract A;
(2) the Rhizoma Fagopyri Dibotryis raffinate after supercritical extraction is again through 60% ethanol extraction, and extracting solution becomes the extractum shape through rotary evaporation, separates through the D101 macroporous adsorbent resin with water dissolution again, and the gained material is an extract B;
Said extracted thing A and extract B are to be had alpha-glucosidase and suppresses active efficient element of golden buckwheat.
2, the present invention is a kind of supercritical CO
2The method of fluid extraction efficient element of golden buckwheat, this method are that the nonpolar invalid components in the Rhizoma Fagopyri Dibotryis and the method for polar invalid components are removed in disposable extraction; It is characterized in that having following technical process and step:
A. with supercritical CO
2Fluid is a solvent, adds the organic solvent dehydrated alcohol simultaneously as entrainer, in extraction kettle Rhizoma Fagopyri Dibotryis is extracted; The particulate granularity of Rhizoma Fagopyri Dibotryis is 28～60 orders; Supercritical CO
2Extracting pressure when fluid extracts is 15～35MPa, and extraction temperature is 35～55 ℃, and the extraction time is 1～3 hour; CO
2Flow be 25～40Kg/h; The flow of entrainer dehydrated alcohol is 1.5～3.0L/h; Then, above-mentioned supercritical fluid extraction thing is carried out resolving, i.e. CO twice
2The fluid band the extract gradation and is entered separating still; The parsing pressure that the first order is resolved is 7～8MPa, and resolution temperature is 50～55 ℃; The parsing pressure that resolve the second level is 5～6MPa, and resolution temperature is 35～40 ℃; The extract of parsing gained is non-level property invalid components and the polar invalid components in the Rhizoma Fagopyri Dibotryis;
B. the further extraction of Rhizoma Fagopyri Dibotryis raffinate is handled behind supercritical extraction: extract to handle and take dual mode, obtain extract A and extract B respectively, two kinds of extracting modes are:
(1) the Rhizoma Fagopyri Dibotryis raffinate after supercritical extraction is again through dehydrated alcohol extraction, and the gained material is an extract A;
(2) the Rhizoma Fagopyri Dibotryis raffinate after supercritical extraction is again through 60% ethanol extraction, and extracting solution becomes the extractum shape through rotary evaporation, separates through the D101 macroporous adsorbent resin with water dissolution again, and the gained material is an extract B;
Said extracted thing A and extract B are to be had alpha-glucosidase and suppresses active efficient element of golden buckwheat.
The inventive method is extracted the material obtain and be can be used for preparation and have alpha-glucosidase and suppress active hypoglycemic medicine.No hazardous solvent is residual in the product of the inventive method, to the human body safety non-toxic.The inventive method technology is simple, and is easy to operate.In the extracting system of the inventive method, CO
2Gas can come back to filter by pipeline again, enters next step extraction work, realizes the circulation repeated use.
Description of drawings
Fig. 1 is a supercritical CO of the present invention
2The fluid extraction process flow diagram.
The specific embodiment
After now specific embodiments of the invention being described in.
Technical process and step in the present embodiment are as follows:
In the phase I, use supercritical CO
2Nonpolar invalid components in the fluid extraction Rhizoma Fagopyri Dibotryis: with granularity behind the crushing screening is that 28～60 purpose Rhizoma Fagopyri Dibotryis materials 200 restrain in the extraction kettle of packing into, with supercritical CO
2Fluid is a solvent; CO
2Send by steel cylinder, behind depurator, cooling coil and basin, liquefy, boost to the extracting pressure 15MPa of setting, to 55 ℃ of the extraction temperature of setting, make CO through heat exchanger heats by high-pressure pump
2Fluid becomes the supercritical fluid state, and its flow is 30kg/h; CO subsequently
2Fluid enters extraction kettle and contacts and extract with the Rhizoma Fagopyri Dibotryis pellet; Extract is at CO
2Fluidic band carries out the first order and resolves with entering first separating still together down, and parsing pressure is 7MPa, and resolution temperature is 50 ℃; Then at CO
2Under the fluidic drive, not resolved extract is entered second separating still again, carry out the second level and resolve, parsing pressure is 6MPa, and resolution temperature is 40 ℃; Resolve the extract of emitting after finishing in the separating still, it is nonpolar invalid components.The extraction time always is about 2 hours.
Then on the basis of phase I, enter second stage extraction: in the second stage, be that the raffinate that will retain in the extraction kettle in the phase I is as extraction feed; The Rhizoma Fagopyri Dibotryis raffinate through phase I accumulation gained is repeatedly taken by weighing 200 grams, in the extraction kettle of packing into, with the organic solvent dehydrated alcohol as entrainer, the further polar invalid components in the extracting gold Semen Fagopyri Esculenti.With supercritical CO
2Fluid is a solvent, CO
2Send by steel cylinder, behind depurator, cooling coil and basin, CO
2Be liquefied, boost to the extracting pressure 15MPa of setting, to 55 ℃ of the extraction temperature of setting, make CO through heat exchanger heats through high-pressure pump
2Become the supercritical fluid state, its flow is 30kg/h; CO subsequently
2Fluid enters extraction kettle, contacts and extracts with the Rhizoma Fagopyri Dibotryis raffinate; Meanwhile, as the organic solvent dehydrated alcohol of entrainer, emit from basin, pump in the extraction kettle by high-pressure pump, the flow of dehydrated alcohol entrainer is 2.5L/h; Organic solvent dehydrated alcohol and CO
2Fluid-mixing extracts in extraction kettle jointly; This moment, extracting pressure was 30MPa, and extraction temperature is 45 ℃; Then above-mentioned hybrid extraction fluid and extract are carried out resolving twice, promptly the fluid-mixing band the extract gradation and is entered first and second separating stills; The pressure that the first order is resolved is 7MPa, and temperature is 50 ℃; The pressure that resolve the second level is 6MPa, and temperature is 40 ℃.Resolve the extract of emitting after finishing in the separating still, it is a polar invalid components.Total extraction time of this second stage is 2 hours.
At last, the Rhizoma Fagopyri Dibotryis raffinate behind supercritical extraction further extracts processing.Extract to handle and take dual mode, obtain extract A and extract B respectively.Two kinds of extracting modes are:
(1) the Rhizoma Fagopyri Dibotryis raffinate after supercritical extraction is again through dehydrated alcohol extraction, and the gained material is an extract A;
(2) the Rhizoma Fagopyri Dibotryis raffinate after supercritical extraction is again through 60% ethanol extraction, and extracting solution becomes the extractum shape through rotary evaporation, separates through the D101 macroporous adsorbent resin with water dissolution again, and the gained material is an extract B;
Said extracted thing A and extract B are to be had alpha-glucosidase and suppresses active efficient element of golden buckwheat.
Embodiment 2
Present embodiment is that the nonpolar invalid components in the Rhizoma Fagopyri Dibotryis and the method for polar invalid components are removed in disposable extraction.The technical process and the step of present embodiment are as follows:
With granularity behind the crushing screening is that 28～60 purpose Rhizoma Fagopyri Dibotryis materials 200 restrain in the extraction kettle of packing into, with supercritical fluid CO
2Be solvent, extractant CO
2Send by steel cylinder, through depurator, cooling coil and basin, CO
2Be liquefied, boost to the extracting pressure 15MPa of setting, to 55 ℃ of the extraction temperature of setting, make CO through heat exchanger heats by high-pressure pump
2Composition is the supercritical fluid state, supercritical CO
2Fluidic flow is 30kg/h; CO subsequently
2Entering extraction kettle contacts and extracts with the Rhizoma Fagopyri Dibotryis material; Meanwhile, as the active solvent dehydrated alcohol of entrainer, emit from basin, pump in the extraction kettle by high-pressure pump, the flow of dehydrated alcohol entrainer is 2.5L/h; Organic solvent dehydrated alcohol and CO
2Fluid-mixing extracts in extraction kettle jointly; This moment, extracting pressure was 30MPa, and extraction temperature is 45 ℃; Then above-mentioned hybrid extraction fluid and extract are carried out resolving twice, promptly the fluid-mixing band the extract gradation and is entered first and second separating stills; The pressure that the first order is resolved is 7MPa, and temperature is 50 ℃; The pressure that resolve the second level is 6MPa, and temperature is 40 ℃.Resolve the extract of emitting after finishing in the separating still, it is nonpolar invalid components and polar invalid components.Total extraction time of this process is 2 hours.
At last, the Rhizoma Fagopyri Dibotryis excess behind supercritical extraction further extracts processing.Extract to handle and take dual mode, obtain extract A and extract B respectively.Two kinds of extracting modes are:
(1) the Rhizoma Fagopyri Dibotryis raffinate after supercritical extraction is again through dehydrated alcohol extraction, and the gained material is an extract A;
(2) the Rhizoma Fagopyri Dibotryis raffinate after supercritical extraction is again through 60% ethanol extraction, and extracting solution becomes the extractum shape through rotary evaporation, separates through the D101 macroporous adsorbent resin with water dissolution again, and the gained material is an extract B;
Said extracted thing A and extract B are to be had alpha-glucosidase and suppresses active efficient element of golden buckwheat.
The operational process of craft of above-mentioned specific embodiment can be referring to the supercritical CO of Fig. 1
2The fluid extraction process flow diagram.
To extracting the experiment of the efficient element of golden buckwheat of gained among the embodiment
One, alpha-glucosidase is suppressed active experiment
Inhibition activity experiment to alpha-glucosidase is the biochemical test whether external check has hypoglycemic activity.To the polar invalid components after the Rhizoma Fagopyri Dibotryis supercritical extraction (C), nonpolar invalid components (D), extract A and extract B, each makes the inhibition activity experiment to alpha-glucosidase.Its experimental result such as table 1:
The different inhibitor of table 1 are to the inhibition activity of alpha-glucosidase
| Inhibitor | Extracting method | IC 50(μg/ml) |
| A | Dehydrated alcohol | 0.231 |
| B | Cross the D101 post | 0.192 |
| C | Supercritical CO 2Adding entrainer extracts | -(non-activity) |
| D | Supercritical CO 2Extract | -(non-activity) |
Two, the inductive blood glucose in diabetic mice value experiment of alloxan
Get normal Kunming mouse, body weight 18～22g, totally 36, male and female half and half, fasting be can't help water 14 hours, lumbar injection alloxan 240mg/kg, fasting be can't help water 8 hours after three days, venous sinus is got blood in 4000rpm/min, gets determination of serum behind the 5min, chooses blood glucose value and experimentizes the above person of 11.1mmol/l.The difference of each treated animal average blood sugar value is not more than 0.5mmol/l during grouping.Be divided into six groups, i.e. negative control group, positive controls, extract A high dose, extract A low dosage, extract B high dose, extract B low dosage.During experiment, negative control group is irritated the stomach normal saline, and positive controls is irritated stomach glucobay (acarbose) 20mg/kg, extract A high dose 200mg/kg, extract A low dosage 100mg/kg, extract B high dose 200mg/kg, extract B low dosage 100mg/kg, successive administration 10d, fasting 8h after the last administration, the eye socket venous sinus is got blood, centrifuging and taking serum, press the determination of glucose oxidase blood sugar level, the results are shown in Table 2:
Table 2 Rhizoma Fagopyri Dibotryis extract is to the influence of the inductive blood glucose in diabetic mice value of alloxan (x ± s)
| Group | Dosage (mg/kg) | The example number | Blood glucose value (mmol/l) |
| Negative control group | -- | 6 | 25.45±10.98 |
| Positive controls | 20 | 6 | 21.81±7.13 * |
| The extract A high dose | 200 | 6 | 18.06±6.54 * |
| The extract A low dosage | 100 | 6 | 23.17±9.54 |
| The extract B high dose | 200 | 6 | 16.82±7.29 * |
| The extract B low dosage | 100 | 6 | 23.49±5.73 |
*P＜0.05v.s negative control group
The inductive blood glucose in diabetic mice value of alloxan laboratory result shows that extract A, B high dose have remarkable result to the blood sugar lowering of diabetes, is that acarbose is effective than positive controls simultaneously.
Claims (2)
1. supercritical CO
2The method of fluid extraction efficient element of golden buckwheat is characterized in that having following technical process and step:
A. phase I: use supercritical CO
2Nonpolar invalid components in the fluid extraction Rhizoma Fagopyri Dibotryis; With supercritical CO
2Fluid is a solvent, in extraction kettle Rhizoma Fagopyri Dibotryis is extracted; The particulate granularity of Rhizoma Fagopyri Dibotryis is 28～60 orders; Extracting pressure is 10～30MPa, and extraction temperature is 35～60 ℃, CO
2Flow is 25～40Kg/h, and the extraction time is 1～3 hour; Extract is at CO
2Fluid enters first separating still under driving, and carries out the first order and resolves, and parsing pressure is 7～8MPa, and resolution temperature is 50～55 ℃; Not resolved extract in the first order is resolved is at CO
2Under the fluidic drive, enter second separating still, carry out the second level and resolve, parsing pressure is 5～6MPa, and resolution temperature is 35～40 ℃; The extract of resolving gained is nonpolar invalid components;
B. second stage: on the phase I basis, add organic solvent, further extract polar invalid components as entrainer; The raffinate retained in the extraction kettle in phase I as extraction feed, is made entrainer with the organic solvent dehydrated alcohol, described raffinate is carried out supercritical CO
2The extraction of fluidic polar invalid components; Extracting pressure is 15～35MPa, and temperature is 35～55 ℃, CO
2Flow is 25～40Kg/h, and the extraction time is 1～3 hour; The flow of entrainer organic solvent dehydrated alcohol is 1.5～3.0L/h; Then, with supercritical CO
2Fluid extract carries out resolving twice by aforementioned the same manner; Be CO
2The fluid band the extract gradation and is entered separating still; The parsing pressure that the first order is resolved is 7～8MPa, and resolution temperature is 50～55 ℃; The parsing pressure that resolve the second level is 5～6MPa, and resolution temperature is 35～40 ℃; The extract of resolving gained is polar invalid components;
C. the further extraction of the Rhizoma Fagopyri Dibotryis raffinate behind supercritical extraction is handled: extract to handle and take dual mode, obtain extract A and extract B respectively, two kinds of extracting modes are:
(1) the Rhizoma Fagopyri Dibotryis raffinate after supercritical extraction is again through dehydrated alcohol extraction, and the gained material is an extract A;
(2) the Rhizoma Fagopyri Dibotryis raffinate after supercritical extraction is again through 60% ethanol extraction, and extract becomes the extractum shape through rotary evaporation, separates through the D101 macroporous adsorbent resin with water dissolution again, and the gained material is an extract B;
Said extracted thing A and extract B are to be had alpha-glucosidase and suppresses active efficient element of golden buckwheat.
2. supercritical CO
2The method of fluid extraction efficient element of golden buckwheat, this method are that the nonpolar invalid components in the Rhizoma Fagopyri Dibotryis and the method for polar invalid components are removed in disposable extraction; It is characterized in that having following technical process and step:
A. with supercritical CO
2Fluid is a solvent, adds the organic solvent dehydrated alcohol simultaneously as entrainer, in extraction kettle Rhizoma Fagopyri Dibotryis is extracted; The particulate granularity of Rhizoma Fagopyri Dibotryis is 28～60 orders; Supercritical CO
2Extracting pressure when fluid extracts is 15～35MPa, and extraction temperature is 35～55 ℃, and the extraction time is 1～3 hour; CO
2Flow be 25～40Kg/h; The flow of entrainer dehydrated alcohol is 1.5～3.0L/h; Then, above-mentioned supercritical fluid extraction thing is carried out resolving, i.e. CO twice
2The fluid band the extract gradation and is entered separating still; The parsing pressure that the first order is resolved is 7～8MPa, and resolution temperature is 50～55 ℃; The parsing pressure that resolve the second level is 5～6MPa, and resolution temperature is 35～40 ℃; The extract of parsing gained is non-level property invalid components and the polar invalid components in the Rhizoma Fagopyri Dibotryis;
B. the further extraction of Rhizoma Fagopyri Dibotryis raffinate is handled behind supercritical extraction: extract to handle and take dual mode, obtain extract A and extract B respectively, two kinds of extracting modes are:
(1) the Rhizoma Fagopyri Dibotryis raffinate after supercritical extraction is again through dehydrated alcohol extraction, and the gained material is an extract A;
(2) the Rhizoma Fagopyri Dibotryis raffinate after supercritical extraction is again through 60% ethanol extraction, and extracting solution becomes the extractum shape through rotary evaporation, separates through the D101 macroporous adsorbent resin with water dissolution again, and the gained material is an extract B;
Said extracted thing A and extract B are to be had alpha-glucosidase and suppresses active efficient element of golden buckwheat.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102406122A (en) * | 2011-11-24 | 2012-04-11 | 神池县粮油机械研究所 | Method for extracting buckwheat nutrient |
| CN102406121A (en) * | 2011-11-24 | 2012-04-11 | 郭盛 | CO2 supercritical extraction method for tartary buckwheat nutrient |
| CN102727593A (en) * | 2012-07-16 | 2012-10-17 | 劲牌有限公司 | New use of wild buckwheat rhizome and wild buckwheat rhizome extract in preparation of hypoglycemic medicine and healthcare food |
| CN106511493A (en) * | 2016-10-14 | 2017-03-22 | 浙江农林大学 | Aromatase inhibitor prepared through plant extracts and manufacturing method of aromatase inhibitor |
| CN108244658A (en) * | 2018-04-20 | 2018-07-06 | 大连军门保健食品有限公司 | A kind of alcohol-relieving and liver-protecting preparation containing witloof nutritional ingredient and preparation method thereof |
| CN112138422A (en) * | 2019-06-28 | 2020-12-29 | 中国科学院理化技术研究所 | Integrated device and method for removing pesticide residues and extracting traditional Chinese medicine |
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2007
- 2007-04-25 CN CN200710039972A patent/CN100586448C/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102406122A (en) * | 2011-11-24 | 2012-04-11 | 神池县粮油机械研究所 | Method for extracting buckwheat nutrient |
| CN102406121A (en) * | 2011-11-24 | 2012-04-11 | 郭盛 | CO2 supercritical extraction method for tartary buckwheat nutrient |
| CN102727593A (en) * | 2012-07-16 | 2012-10-17 | 劲牌有限公司 | New use of wild buckwheat rhizome and wild buckwheat rhizome extract in preparation of hypoglycemic medicine and healthcare food |
| CN106511493A (en) * | 2016-10-14 | 2017-03-22 | 浙江农林大学 | Aromatase inhibitor prepared through plant extracts and manufacturing method of aromatase inhibitor |
| CN108244658A (en) * | 2018-04-20 | 2018-07-06 | 大连军门保健食品有限公司 | A kind of alcohol-relieving and liver-protecting preparation containing witloof nutritional ingredient and preparation method thereof |
| CN108244658B (en) * | 2018-04-20 | 2021-08-20 | 大连军门保健食品有限公司 | An anti-hangover and hepatoprotective agent containing herba Cichorii nutrients, and its preparation method |
| CN112138422A (en) * | 2019-06-28 | 2020-12-29 | 中国科学院理化技术研究所 | Integrated device and method for removing pesticide residues and extracting traditional Chinese medicine |
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| Publication number | Publication date |
|---|---|
| CN100586448C (en) | 2010-02-03 |
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