CN101021523A - Method for detecting joint liquor used for diagnosing early bony arthritis - Google Patents
Method for detecting joint liquor used for diagnosing early bony arthritis Download PDFInfo
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- CN101021523A CN101021523A CN 200610033628 CN200610033628A CN101021523A CN 101021523 A CN101021523 A CN 101021523A CN 200610033628 CN200610033628 CN 200610033628 CN 200610033628 A CN200610033628 A CN 200610033628A CN 101021523 A CN101021523 A CN 101021523A
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Abstract
This invention discloses a kind of joint fluid detection method to detect early stage bony arthritis. Firstly, centrifugating the joint fluid of the suspected arthritic into two layer, diluting the upper liquor with isotonic glucose solution, measuring the size of the nanometer grain and the Zeta potential by using the quasi-elastic laser light scattering technology and phase analysis electrophoresis light scattering technology, analyzing the underlayer by many-dimension micro imaging technology, digital processing and image analysis technology, measuring the shape of the nanometer grain and analyzing the parameters, including the contact area, perimeter, the long axis, the minor axis, shape naturalized factor, extensibility, tightness, integral optical density, the number of the nanometer grain in unit area. According to the size of the nanometer grain and the Zeta potential as well as the distribution situation of the nanometer grain size, judging whether the detected joint fluid is early stage bony arthritis. The invention can also be applied to detect other joint diseases and its cure evaluation.
Description
Technical field
The present invention designs a kind of joint fluid detection method that is used to diagnose early stage Osteoarthritis.
Background technology
Be accompanied by the formation of aged society, the incidence of the various diseases of old people especially incidence of bone and joint diseases is increasing rapidly.Osteoarthritis has been a modal flesh bone source property disability-causing factor in developed country, thereby simultaneously joint disease causes that death incidence in various diseases that complication causes is the highest.According to statistics, at present the patient who only suffers from osteoarthritis in the U.S. has 4,000 ten thousand crowd, expects the year two thousand twenty and will reach 6,000 ten thousand.In China, only the incidence of disease of osteoarthritis is also up to 4%, and in city retirement crowd more up to 49%.Along with the people increases day by day to the requirement of quality of life, the early diagnosis of joint disease, treatment and control.The middle and advanced stage of bone and joint diseases will cause arthralgia, distortion, and moving obstacle causes the patient to live and spiritual huge misery.Therefore, early diagnosis, developing of treatment and prevention joint disease prevents trouble before it happens, and is that clinic study is badly in need of one of key subjects that solve.
During relatively concealment of the generation of joint disease development, most of joint disease have reached when diagnosis, late period, missed the Best Times of treatment.And present existing inspection means are mainly according to clinical symptoms, health check-up, X radiodiagnosis x, the inspection of articular cartilage nuclear-magnetism etc.But health check-up, conventional joint are checked, the X line is taken the photograph sheet etc. all can't detect the early stage pathological change in joint, also can't judge its prognosis situation.Along with development of science and technology, MR checks and has obtained widespread use in arthritic early diagnosis, yet MR checks to the variation of environment in the joint due to the early stage articular cartilage pathology and not as the joint fluid inspection, its sensitivity is far below the inspection of joint fluid, and MR checks and to cost an arm and a leg, can not be as the technological means of generaI investigation.High sensitivity and high diagnosis specificity so if can develop, and the joint fluid detection technique of relevant pathological change in fast detecting joint in early days and prognosis thereof, then not only significant on clinical analysis of diagnosis, but also have good generaI investigation meaning.
Summary of the invention
The object of the present invention is to provide a kind of joint fluid detection method that is used to diagnose early stage Osteoarthritis, can carry out comprehensive qualitative and detection by quantitative nano particle in doubtful early stage Osteoarthritis patient's the joint fluid and micron particles.
In order to achieve the above object, the technical solution used in the present invention is as follows:
One, the assay method that is used for the joint fluid nano particle
Adopt micro-syringe tapping method to obtain joint fluid, and adopt unified dilution (0.9%NaCL 10mL) pumping back method to obtain joint fluid.The joint fluid gathered is centrifugal with 2000 rev/mins speed in 4 ℃ the time, get the centrifuge tube upper strata, nano particle is wherein measured and analyzed.At first, ooze the joint fluid on D/W dilution centrifuge tube upper strata with 5% grade, wherein the volume ratio of joint fluid and 5% glucose is 3: 20.Make uniform solution after at first two kinds of liquid being mixed by a certain percentage, left standstill 15 minutes, remove the bubble in the solution, with the granule size of nano particle in the quasielastic laser light scattering commercial measurement solution, measuring temperature is 37 ℃.Secondly with the Zeta potential of facies analysis electrophoretic light scattering commercial measurement nano particle, measure temperature and also be 37 ℃.
Two, the assay method that is used for the joint fluid micron particles
Lower floor with above-mentioned joint fluid after centrifugal measures and analyzes micron particles wherein.Utilize the multidimensional micro-imaging technique that the micron particles in the joint fluid is observed and taken earlier, utilize digital processing technology and image analysis technology to combine again, form and size parameter to captured micron particles are analyzed, and comprise the number of micron particles in contact area, girth, major axis, minor axis, shape entraining agent, length growth rate, tight ness rating, integral optical density, the unit area.Contact area (Contact area) and girth (Perimeter) are the two dimension tolerance of micron particles, and its value has reflected the size of micron particles.Major axis (Major axislength) and minor axis (Minor axis length) are the one dimension tolerance of joint fluid micron particles, can reflect the size of micron particles.The geometric shape of shape entraining agent (Roundness) reflection micron particles, its value approach the approaching more circle of 1 expression micron particles more, and its value equals 1 expression micron particles for circular.Length growth rate (Elongation) also is an important parameter of reflection micron particles geometric shape, and its numerical value is more than or equal to 1, and when its numerical value equaled 1, the expression micron particles be circular, when its numerical value greater than 1 the time, represent that micron particles is strip or clavate.The parameter such as the tight ness rating (Compactness) that reflect other characteristic of micron particles in addition in addition, the fine and close situation of expression joint fluid micron particles, the big more expression micron particles of its numerical value is fine and close more, and the more little expression micron particles of numerical value is loose more.The printing opacity situation of integral optical density (Integrated density) expression micron particles, easy more the seeing through of the more little expression light of its value, the big more expression light of its value is not easy to see through more.The situation of change that also can reflect in addition, micron particles quantity in the joint fluid by the number of micron particles in the unit of account area (each ten thousand square micron).
The advantage that the present invention has is: the present invention utilizes multidimensional micro-imaging and image analysis technology, quasielastic laser light scattering technology and facies analysis electrophoretic light scattering technology, the micron particles in the mensuration joint fluid and the granule size and the Zeta potential of nano particle, have advantages such as detection method is easy, reliable, testing cost is low, popularize easily, be the method for the clinical early diagnosis and therapy prognosis evaluation of a kind of knee joint osseous arthritis, the early detection that also can be used for other joint disease is in treatment effectiveness evaluation.
Embodiment
The granule size and the Zeta potential of the present invention's nano particle in obtaining joint fluid distributes, the granule size of micron particles distributes.The particle size range of nano particle is divided into mean grain size less than 200 nanometers, greater than 300 nanometers, between 200 nanometers and 300 nanometers three groups.With the particle size range of micron particles be divided into less than 10 microns, greater than 100 microns, between 10 microns and 100 microns totally three groups.With the Zeta potential scope of nano particle be divided into less than 15 millivolts, greater than 20 millivolts, between 15 millivolts and 20 millivolts.If take out according to the method described above certain patient's joint fluid, and handle as stated above and measure, the size-grade distribution that obtains nano particle in the joint fluid is being higher than other two groups less than the shared number percent of 200 nanometer groups, the Zeta potential of nano particle is distributed in less than 15 millivolts of shared number percents of group and is higher than other two groups, and the size-grade distribution of micron particles can judge tentatively that also being higher than other two groups less than 10 microns shared number percents of group this joint may suffer from early stage Osteoarthritis.
Claims (4)
1, a kind of joint fluid detection method that is used to diagnose early stage Osteoarthritis, it is characterized in that: be centrifugated into doubtful arthritic's joint fluid two-layer earlier, respectively the contained contained micron particles of nano particle and lower floor in upper strata is detected, and in conjunction with the comprehensive possibility of judging early stage Osteoarthritis of the testing result of all joint fluid nanometers and micron particles.
2, the upper solution that contains nano particle in the claim 1 with 5% etc. ooze the D/W dilution, wherein the volume ratio of upper solution and 5% glucose is 3: 20.Use quasielastic laser light scattering technology and facies analysis electrophoretic light scattering the commercial measurement wherein granule size and the Zeta potential of nano particle then respectively, measure temperature and be 37 ℃.
3, the lower floor that contains micron particles in the claim 1, directly utilize the multidimensional micro-imaging technique that micron particles is wherein observed and taken, utilize digital processing technology and image analysis technology to combine again, form and size parameter to captured micron particles are analyzed, and comprise the number of micron particles in contact area, girth, major axis, minor axis, shape entraining agent, length growth rate, tight ness rating, integral optical density, the unit area.
4, granularity and the Zeta potential according to the nano particle in claim 2 and 3 distributes, and in conjunction with the size-grade distribution situation of the micron particles in the claim 3, judges whether the joint fluid of checking belongs to early stage Osteoarthritis.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610033628 CN101021523A (en) | 2006-02-16 | 2006-02-16 | Method for detecting joint liquor used for diagnosing early bony arthritis |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610033628 CN101021523A (en) | 2006-02-16 | 2006-02-16 | Method for detecting joint liquor used for diagnosing early bony arthritis |
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| CN101021523A true CN101021523A (en) | 2007-08-22 |
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| CN 200610033628 Pending CN101021523A (en) | 2006-02-16 | 2006-02-16 | Method for detecting joint liquor used for diagnosing early bony arthritis |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102323191A (en) * | 2008-09-26 | 2012-01-18 | 株式会社堀场制作所 | Device for measuring physical property of particle |
| CN103914695A (en) * | 2014-04-25 | 2014-07-09 | 中山大学 | Device and method for micro-electrophoresis image recognition |
| CN104880487A (en) * | 2015-05-19 | 2015-09-02 | 中山大学 | Fiber surface Zeta potential measurement method and device based on image identification |
-
2006
- 2006-02-16 CN CN 200610033628 patent/CN101021523A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102323191A (en) * | 2008-09-26 | 2012-01-18 | 株式会社堀场制作所 | Device for measuring physical property of particle |
| CN102323191B (en) * | 2008-09-26 | 2013-11-06 | 株式会社堀场制作所 | Device for measuring physical property of particle |
| US8625093B2 (en) | 2008-09-26 | 2014-01-07 | Horiba, Ltd. | Particle characterization device |
| CN103914695A (en) * | 2014-04-25 | 2014-07-09 | 中山大学 | Device and method for micro-electrophoresis image recognition |
| CN103914695B (en) * | 2014-04-25 | 2017-01-11 | 中山大学 | Device and method for micro-electrophoresis image recognition |
| CN104880487A (en) * | 2015-05-19 | 2015-09-02 | 中山大学 | Fiber surface Zeta potential measurement method and device based on image identification |
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