CN101014710A - CAEV-based vector system - Google Patents

CAEV-based vector system Download PDF

Info

Publication number
CN101014710A
CN101014710A CNA2004800439386A CN200480043938A CN101014710A CN 101014710 A CN101014710 A CN 101014710A CN A2004800439386 A CNA2004800439386 A CN A2004800439386A CN 200480043938 A CN200480043938 A CN 200480043938A CN 101014710 A CN101014710 A CN 101014710A
Authority
CN
China
Prior art keywords
carrier
less
cell
carrier system
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CNA2004800439386A
Other languages
Chinese (zh)
Inventor
李修京
金连洙
金钟必
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
MACROGEN CO Ltd
Original Assignee
MACROGEN CO Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by MACROGEN CO Ltd filed Critical MACROGEN CO Ltd
Publication of CN101014710A publication Critical patent/CN101014710A/en
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/10041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/10043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/108—Plasmid DNA episomal vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2810/00—Vectors comprising a targeting moiety
    • C12N2810/50—Vectors comprising as targeting moiety peptide derived from defined protein
    • C12N2810/60—Vectors comprising as targeting moiety peptide derived from defined protein from viruses
    • C12N2810/6072—Vectors comprising as targeting moiety peptide derived from defined protein from viruses negative strand RNA viruses
    • C12N2810/6081—Vectors comprising as targeting moiety peptide derived from defined protein from viruses negative strand RNA viruses rhabdoviridae, e.g. VSV
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/38—Vector systems having a special element relevant for transcription being a stuffer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Engineering & Computer Science (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The invention relates to caprine arthritis encephalitis virus-based vectors and vector systems that are useful in the delivery of nucleic acids to both non-dividing and dividing cells. Methods for delivering nucleic acids to both non-dividing and dividing cells using the vector systems are also disclosed.

Description

Carrier system based on CAEV
Technical field
The present invention relates to be used to send the lentiviral vectors of polynucleotide, relate more specifically to be used for send the carrier based on arthritis-Encephalitis virus of polynucleotide to non-division and somatoblast.
Background technology
Slow virus is a retroviral subgroup, can infect non-division and somatoblast.Since derive from the carrier of slow virus can stable integration in the genome of division and Unseparated Cell and can mediate long-term genetic expression, so it is ideal tools (Gilbert and Wong-Staal, 2001 of sending foreign gene to target cell; Mitrophanous et al., 1999; Naldini et al., 1996; Sauter and Gasmi, 2001).
Comprised primate and the non-human primate animal and isolated slow virus from a lot of invertebrate species, in the primate as people and simian immunodeficiency virus (HIV-1, HIV-2, SIV), and in the non-human primate animal as feline immunodeficiency virus (FIV), bovine immunodeficiency virus (BIV), equine infectious virus (EIAV), arthritis-Encephalitis virus (CAEV) and visna virus (visna virus).Wherein, understanding maximum at present is HIV and SIV.Yet,, caused serious safety issue so this system is used for philtrum because carrier might reassemble into form virulence, pathogenic.Therefore, preferred non-human primate slow virus is used for gene therapy.
In the non-human primate lentiviral vectors, to FIV (Curran and Nolan, 2002) and EIAV[US2001/0044149] come the feature description of source carrier maximum, and the progress of other non-human primate lentiviral vectorss is less.
As all slow viruss, CAEV can infect in somatoblast and in differentiation of whole end and Unseparated Cell and duplicate.The various biological character of CAEV becomes this virus to develop into the noticeable candidate of transgenosis/treatment carrier.At first, the normal host of CAEV is a goat, and does not have the case of the remarkable CAEV of infection of report.Secondly, the CAEV genome be in the slow virus apart from the generation of HIV-1 system farthest.Once more, compare with other slow viruss, the composition of genome of CAEV is simple relatively.The CAEV genome contains 3 structure genes (gag, pol, env) and 3 adjusting/auxiliary genes (vif, tat and rev).
Although these advantages are arranged, the not success of effort that exploitation is done based on the delivery system of CAEV has only produced dangerous and recombinant viral vector production system poor efficiency, and this makes that use is unactual based on the genes delivery system of CAEV.
1998, Mselli-Lakhal et al. reported the carrier system of the first-generation based on CAEV, but the virus titer of this system (that is: 10-187TU/ml) is below available horizontal.The author is low owing to the packaging efficiency of geneome RNA accumulation and vector rna in the shortage kytoplasm with poor efficiency.Another shortcoming of this research be with infectious wild-type virus (" helper virus ") as its packaging system, this seldom has actual value being used for man-hour.
Therefore, still need a kind ofly safely and efficiently based on the slow virus carrier system of CAEV, it can mediate the division of wide scope and the transgenosis in the Unseparated Cell.
Summary of the invention
Relate generally to production of the present invention is used for sending to target cell the lentiviral vectors particle based on CAEV of external source polynucleotide.These carrier granules can be used for antiviral, antitumor and/or gene therapy.
On the one hand, the invention provides and be used for the transfer vector based on CAEV carrier production system described herein, this transfer vector comprises (a) CAEV packaging sequence, this packaging sequence is made up of following substantially: (i) non-translational region between CAEV5 ' LTR and the CAEV gag-encoding sequence, and the Nucleotide 1 of CAEV gag-encoding sequence that (ii) is connected to described non-translational region 3 ' end is to X, wherein X is less than 613, (b) polyadenylic acidization, rna transport, reverse transcription and integration required and form the cis-acting elements that operability is connected with described packaging sequence.
In one embodiment of the invention, X is selected from 60,100,125,150,175,200,225,250,275,300,325,350,375,400,425,450,475,500,525,550,575 and 600.
In another embodiment of the invention, X is selected from:
(a) X is greater than 25 and less than 600,
(b) X is greater than 25 and less than 500,
(c) X is greater than 25 and less than 400,
(d) X is greater than 25 and less than 300,
(e) X is greater than 25 and less than 200,
(f) X is greater than 50 and less than 600,
(g) X is greater than 50 and less than 500,
(h) X is greater than 50 and less than 400,
(i) X is greater than 50 and less than 300,
(j) X is greater than 50 and less than 200,
(k) X is greater than 75 and less than 600,
(l) X is greater than 75 and less than 500,
(m) X is greater than 75 and less than 400,
(n) X is greater than 75 and less than 300,
(o) X is greater than 75 and less than 200,
(p) X is greater than 100 and less than 600,
(q) X is greater than 100 and less than 500,
(r) X is greater than 100 and less than 400,
(s) X is greater than 100 and less than 300,
(t) X is greater than 100 and less than 200,
(u) X is greater than 125 and less than 600,
(v) X is greater than 125 and less than 500,
(w) X is greater than 125 and less than 400,
(x) X is greater than 125 and less than 300,
(y) X is greater than 125 and less than 200,
(z) X is greater than 150 and less than 600,
(aa) X is greater than 150 and less than 500,
(bb) X is greater than 150 and less than 400,
(cc) X is greater than 150 and less than 300,
(dd) X is greater than 150 and less than 200,
(ee) X is greater than 200 and less than 600,
(ff) X is greater than 200 and less than 500,
(gg) X is greater than 200 and less than 400,
(hh) X is greater than 200 and less than 300,
(ii) X is greater than 200 and less than 200,
(jj) X is greater than 250 and less than 600,
(kk) X is greater than 250 and less than 500,
(ll) X greater than 250 and less than 400 and
(mm) X is greater than 250 and less than 300.
In another embodiment, X is greater than 40 and less than 613.
In another embodiment, X is greater than 57 and less than 613.
In another embodiment, X is about 327.
In one embodiment of the invention, the initiator codon of gag-encoding sequence is suddenlyd change to stop the proteic translation of gag.In another embodiment, initiator codon is mutated into TAG.
In another embodiment of transfer vector of the present invention, the ATG codon of gag-encoding sequence is positioned at initiator codon ATG downstream X base pair, and wherein initiator codon is suddenlyd change stoping the proteic translation of gag, and wherein X less than 30.X is about 21 in another embodiment.
Transfer vector of the present invention can also comprise the RRE district.
In another embodiment of the invention, transfer vector comprises CAEV3 ' LTR, and wherein the U3 district is lacked.
Transfer vector of the present invention also can comprise allogeneic promoter.In one embodiment of the invention, allogeneic promoter is the main immediate early promoter (HCMV MIEP) of human cytomegalic inclusion disease virus.In another embodiment, transfer vector is pCAH/SINd1 (SEQ ID NO:68).
Transfer vector of the present invention also can comprise the box of transcribing that contains allos polynucleotide interested, and its operability is connected to allogeneic promoter (as the main immediate early promoter MCMV MIEP of human cytomegalic inclusion disease virus main immediate early promoter HCMV MIEP or murine cytomegalovirus).This transfer vector allows polynucleotide interested to be incorporated in the virion, thereby provides amplification to contain the method for the infected host cell number of this polynucleotide.
The present invention also provides the slow virus carrier system based on CAEV, its be used to produce can be used for to mammalian cell send the external source polynucleotide based on CAEV, the replication defective carrier granule.The carrier granule mammalian cell that can infect and transduce.Carrier system comprises above-mentioned transfer vector and package carrier system, and wherein said package carrier system comprises: comprise first polynucleotide of CAEVgag-pol-encoding sequence and RRE, and second polynucleotide that comprises the peplos encoding sequence.
In one embodiment, second polynucleotide comprises non--CAEV env-encoding sequence.In one embodiment, second polynucleotide comprises VSV-G or GaLV-encoding sequence.
In another embodiment, the CAEV carrier system comprises the 3rd polynucleotide that contains the rev-encoding sequence.
In another embodiment, the CAEV carrier system comprises the 4th polynucleotide that contains the vif-encoding sequence.
In another embodiment, first polynucleotide of above-mentioned every kind of CAEV carrier system also comprises the allos adjusting sequence that operability is connected to CAEV gag-pol-encoding sequence.
In another embodiment, second polynucleotide of above-mentioned every kind of CAEV carrier system also comprises the allos adjusting sequence that operability is connected to described peplos-encoding sequence.
In another embodiment, the 3rd polynucleotide also comprises the allos adjusting sequence of rev-encoding sequence.
In another embodiment, the 3rd polynucleotide also comprises the allos adjusting sequence that operability is connected to the vif-encoding sequence.
In one embodiment of the invention, the CAEV carrier system comprises the package carrier system that disappearance has the CAEV packaging sequence of function.In another embodiment, the 5 ' end of the CAEV genome between package carrier system disappearance donor splicing site and the gag initiator codon.
In one embodiment, the CAEV carrier system comprises first carrier that contains first polynucleotide and second carrier that contains second polynucleotide.In another embodiment, carrier system comprises first carrier that contains first polynucleotide, contains second carrier of second polynucleotide and contains the 3rd carrier of the 3rd polynucleotide.In another embodiment, carrier system comprises first carrier, second carrier that contains second polynucleotide that contain first polynucleotide, contains the 3rd carrier of the 3rd polynucleotide and contains the 4th carrier of the 4th polynucleotide.The 3rd carrier can be pHYK/rev (SEQ ID NO:75), and the 4th carrier can be pHYK/vif (SEQ ID NO:76).
In another embodiment, carrier system comprise contain first polynucleotide, the 3rd polynucleotide and the 4th polynucleotide first carrier and second carrier that contains second polynucleotide.
In one embodiment, first carrier of CAEV carrier system comprises CAEV gag-encoding sequence and the RRE that operability is connected to allogeneic promoter.In another embodiment, the CAEV carrier system comprises the first carrier pMGP/RRE (SEQ ID NO:77).
In one embodiment, second carrier of CAEV carrier system is the VSV-G-encoding sequence that operability is connected to allogeneic promoter.This promotor can be HCMV MIEP.Second carrier also can comprise the beta-globin intron.In another embodiment, the CAEV carrier system comprises the second carrier pHGVSV-G (SEQ ID NO:74).
In one embodiment, second carrier of CAEV carrier system is the GaLV env-encoding sequence that operability is connected to allogeneic promoter.This promotor can be MCMV MIEP.Second carrier also can comprise eucaryon elongation factor-1alpha intron.In another embodiment, the CAEV carrier system comprises the second carrier pMYKEF-1/env (SEQ ID NO:72).
Another aspect of the present invention is to produce the lentiviral vectors particulate method based on CAEV that can be used for mammalian cell-infecting.This method comprises (a) under be fit to producing based on CAEV particulate condition, and with aforementioned bearer system transfectional cell, wherein carrier granule has and infects and transduction ability and be replication defect type, and (b) reclaims carrier granule.
The present invention also provides and comprises based on the CAEV lentiviral vectors particle and the composition of mediator (carrier) randomly, and wherein carrier granule prepares by preceding method.
The present invention also provides and comprises aforementioned transfer vector or based on the medicine box of CAEV slow virus carrier system.
The present invention also provides the packing cell that comprises CAEV gag-pol-encoding sequence and RRE and randomly viral env-encoding sequence.Packing cell also can comprise rev-encoding sequence and/or vif-encoding sequence.This cell can be used for rna form with transfer vector and is packaged into to have and infects and transduction ability and be in the carrier granule of replication defect type.
In one embodiment, carrier system comprises the cell that contains aforementioned first polynucleotide.Carrier system also can contain the aforementioned the 3rd and/or the 4th polynucleotide.
In another embodiment, carrier system comprises the cell that contains aforementioned first polynucleotide and second polynucleotide.Carrier system also can contain the aforementioned the 3rd and/or the 4th polynucleotide.
In another embodiment, carrier system comprises the cell that contains first carrier, and described first carrier contains CAEV gag-pol-encoding sequence and RRE.Described first carrier also can contain rev-coding and/or vif-encoding sequence.As an alternative, described cell can comprise first carrier that contains CAEV gag-pol-encoding sequence and RRE, contains second carrier of rev-encoding sequence and/or contain the 3rd carrier of vif-encoding sequence.
In some embodiment, carrier system comprises a kind of cell, and described cell comprises first carrier that contains CAEVgag-pol-encoding sequence and RRE and second carrier that contains viral env-encoding sequence.First carrier also can contain rev-coding and/or vif-encoding sequence.As an alternative, described cell can comprise first carrier of containing CAEV gag-pol-encoding sequence and RRE, contains second carrier of viral env-encoding sequence and randomly contain the 3rd carrier of rev-encoding sequence and/or contain the 4th carrier of vif-encoding sequence.
Another aspect of the present invention is the method for sending the polynucleotide molecule of polynucleotide or polypeptide or the described polypeptide of replica code to mammalian cell, and it is included in and can allows described polynucleotide to be incorporated under the condition in the described cellular genome and randomly under the condition that allows amplification by the described polypeptide of described polymerized nucleoside acid encoding mammalian cell to be contacted with the aforementioned bearer particle.Described mammalian cell can be somatoblast, Unseparated Cell or CD34+ stem cell.This also can be included in carrier granule exposing cell isolated cell from Mammals before to the method that mammalian cell is sent the polynucleotide of polynucleotide or polypeptide or the described polypeptide of replica code.After this method also can be included in cell and carrier granule contacts, the described cell of amplification in cultivation.This method also can be included in to increase and before or after the exposing cell it be introduced in the Mammals again.
The present invention also provides the method for sending polypeptide to vertebrates, it comprises that the lentiviral vectors particle based on CAEV that will contain allos polynucleotide interested is applied to vertebrates, wherein carrier granule prepares by preceding method, make and in this vertebrates, express that its expression amount is enough to be detected or cause biologically in this vertebrates by sending polynucleotide encoded polypeptides.
The present invention also provides the carrier that contains the CAEV packaging sequence, wherein the CAEV packaging sequence is made up of following: (a) non-translational region between CAEV5 ' LTR and the CAEV gag-encoding sequence, (b) Nucleotide 1 of CAEV gag-encoding sequence that is connected to described non-translational region 3 ' end is to X, and wherein X is less than 613.
The contriver has been found that as described here, produces described lentiviral vectors particle based on CAEV, has strengthened efficient and security in the lentiviral vectors design with respect to existing carrier granule based on CAEV.By finding that 5 ' LTR has strengthened efficient apart from the optimal length of non-translational region between gag initiator codon and the gag-coding region, thereby it is used as packaging sequence and enhanced virus titre efficiently by allowing to wrap into capsid efficiently.In the plasmid that designs a package by using strong allogeneic promoter also to improve virus titer.By making up tat-dependent/non-dependent transfer vector and having strengthened security based on the packaging system of plasmid.
Description of drawings
Fig. 1 represents the synoptic diagram of CAEV provirus genome structure.
Fig. 2 A represents the synoptic diagram of plasmid pMGP/RRE (SEQ ID NO:77).PMGP/RRE (SEQID NO:77) is the plasmid of a 9446bp, comprises the MCMV MIEP district (bp1-660), RRE district (bp5246-5627 or bp5368-5669) and Trobest (BGH) polyadenylation signal (bp5751-5984) that are positioned at upstream, CAEV gag-pol-coding region (being positioned at bp709-5243).Carrier also comprises neomycin resistance gene coding region (bp8151-7155), SV40 replication origin (bp8509-8152), Col E1 replication origin (bp6115-6698) and ampicillin resistance gene district (bp9362-8528).
Fig. 2 B represents the synoptic diagram of plasmid pMGP/REV/RRE.PMGP/REV/RRE is the plasmid of a 9924bp, comprises MCMV MIEP district (being positioned at bp1-660) and is positioned at the main donor splicing site of CAEV (bp688-704), the first exon rev coding region (bp5383-5494), RRE district (bp5540-5841), the second exon rev coding region (bp5888-6177) and Trobest (BGH) polyadenylation signal (bp6229-6462) of upstream, CAEV gag-pol coding region (bp726-5258).Carrier also comprises neomycin resistance gene coding region (bp7633-8629), SV40 replication origin (bp8987-8630), Col E1 replication origin (bp6593-7176) and ampicillin resistance gene district (bp9840-9006).
Fig. 3 A represents the synoptic diagram of plasmid pCAH/SINd (SEQ ID NO:73).PCAH/SINd (SEQID NO:73) is the plasmid of a 3566bp, comprises CAEV3 ' the LTR district (bp1275-1458) of R-U5 sequence area (bp611-772), RRE district (bp796-1154) and U3 disappearance among HCMV MIEP (bp1-588), the CAEV5 ' LTR.Carrier also comprises Col E1 replication origin (bp1863-2466) and kalamycin resistance gene coding region (bp2698-3510).
Fig. 3 B represents the synoptic diagram of plasmid pCAH/SINd0 (SEQ ID NO:67).PCAH/SINd0 (SEQ ID NO:67) is the plasmid of a 3911bp, comprises CAEV3 ' the LTR district (bp1620-1803) of the R-U5 sequence area (bp611-772) of HCMV MIEP (bp1-588), CAEV5 ' LTR, the residue non-translated sequence (bp773-789) that contains primer binding site (PBS), RRE district (bp1141-1499) and U3 disappearance.Carrier also comprises Col E1 replication origin (bp2208-2791) and kalamycin resistance gene coding region (bp3043-3855).
Fig. 3 C represents the synoptic diagram of plasmid pCAH/SINd1 (SEQ ID NO:68).PCAH/SINd1 (SEQ ID NO:68) is the plasmid of a 4238bp, comprise HCMV MIEP promotor (bp1-588), the R-U5 sequence area (bp610-772) of CAEV5 ' LTR, the residue non-translated sequence (bp773-789) that contains the PBS site, the 327bp fragment (bp1121-1448) of gag gene, wherein the initial ATG codon of this fragment (bp1121-1123) has the ATG point mutation with the ATG codon (bp1142-1144) that is positioned at this initial ATG codon downstream and becomes TAG, CAEV3 ' the LTR district (bp1947-2130) of RRE district (bp1468-1826) and U3 disappearance.Carrier also comprises Col E1 replication origin (bp2535-3118) and kalamycin resistance gene district (bp3370-4182).
Fig. 3 D represents the synoptic diagram of plasmid pCAH/SINd2 (SEQ ID NO:69).PCAH/SINd2 (SEQ ID NO:69) is the plasmid of a 4523bp, comprise HCMV MIEP (bp1-588), CAEV5 ' LTR R-U5 sequence area (bp610-772), contain the residue non-translated sequence (bp773-789) in PBS site, the 612bp fragment (bp1121-1733) of gag gene, initial ATG codon of this fragment (bp1121-1123) and the ATG codon (bp1142-1144) that is positioned at initial ATG codon downstream CAEV3 ' LTR district (bp2232-2415) wherein with point mutation, RRE district (bp1753-2111) and U3 disappearance.Carrier also comprises Col E1 replication origin (bp2820-3403) and kalamycin resistance gene coding region (bp3655-4467).
Fig. 3 E represents the synoptic diagram of plasmid pCAH/SINd3 (SEQ ID NO:70).PCAH/SINd3 (SEQ ID NO:70) is the plasmid of a 4819bp, comprise HCMV MIEP (bp1-588), CAEV5 ' LTR R-U5 sequence area (bp610-772), contain the residue non-translated sequence (bp773-789) in PBS site, the 908bp fragment (bp1121-2029) of gag gene, initial ATG codon of this fragment (bp1121-1123) and the ATG codon (bp1142-1144) that is positioned at initial ATG codon downstream CAEV3 ' LTR district (bp2549-2711) wherein with point mutation, RRE district (bp2049-2407) and U3 disappearance.Carrier also comprises Col E1 replication origin (bp3116-3699) and kalamycin resistance gene coding region (bp3951-4763).
Fig. 3 F represents the synoptic diagram of plasmid pCAH/SINd4 (SEQ ID NO:71).PCAH/SINd4 (SEQ ID NO:71) is the plasmid of a 5112bp, comprise HCMV MIEP (bp1-588), CAEV5 ' LTR R-U5 sequence area (bp610-772), contain the residue non-translated sequence (bp773-1120) in PBS site, the 1198bp fragment (bp1121-2319) of gag gene, initial ATG codon of this fragment (bp1121-1123) and the ATG codon (bp1142-1144) that is positioned at initial ATG codon downstream CAEV3 ' LTR district (bp2842-3004) wherein with point mutation, RRE district (bp2342-2700) and U3 disappearance.Carrier also comprises Col E1 replication origin (bp3409-3992) and kalamycin resistance gene coding region (bp4244-5056).
Fig. 3 G represents the synoptic diagram of plasmid pCAH/SINd1/hlacZ (SEQ ID NO:79).PCAH/SINd1/hlacZ (SEQ ID NO:79) is the plasmid of a pCAH/SINd1 (SEQ ID NO:68) the long 8127bp of deutero-, and it expresses the lacZ reporter gene.This carrier comprises two HCMV MIEP promoter regions (laying respectively at bp1-588 and bp1866-2460), the R-U5 sequence area (bp610-772) of CAEV5 ' LTR, the residue non-translated sequence (bp773-789) that contains the PBS site, the 325bp fragment (bp1121-1446) of gag gene, wherein initial ATG codon of this fragment (bp1121-1123) and the ATG codon (bp1142-1144) that is positioned at initial ATG codon downstream have point mutation, RRE district (bp1466-1836), CAEV3 ' the LTR district (bp5782-6019) of lacZ gene coded sequence (bp2541-5711) and U3 disappearance.Carrier also comprises Col E1 replication origin (bp6424-7007) and kalamycin resistance gene coding region (bp7259-8071).
Fig. 3 H represents the synoptic diagram of plasmid pCAH/SINd60/hlacZ (SEQ ID NO:78).PCAH/SINd60/hlacZ (SEQ ID NO:78) is the plasmid of a long 7856bp, it comprises two promoter regions, HCMV MIEP (laying respectively at bp1-588 and bp1595-2189), the R-U5 sequence area (bp610-772) of CAEV5 ' LTR, the residue non-translated sequence (bp773-789bp) that contains the PBS site, the 60bp fragment (bp1121-1181) of gag gene, wherein initial ATG codon of this fragment (bp1121-1123) and the ATG codon (bp1142-1144) that is positioned at initial ATG codon downstream have point mutation, RRE district (bp1195-1565), CAEV3 ' the LTR district (bp5511-5748) of lacZ gene coded sequence (bp2270-5440) and U3 disappearance.Carrier also comprises Col E1 replication origin (bp6153-6736) and kalamycin resistance gene coding region (bp6988-7800).
Fig. 4 represents the synoptic diagram of plasmid pHYK/vif (SEQ ID NO:76).PHYK/vif (SEQ IDNO:76) is the plasmid of a long 5729bp, and it comprises HCMV MIEP (bp1-596), vif gene coding region (bp691-1380), BGH polyadenylation signal (bp1467-1695), Col E1 replication origin (bp1826-2409), neomycin resistance gene coding region (bp3862-2866) and ampicillin resistance gene coding region (bp5270-4239).
Fig. 5 represents the synoptic diagram of plasmid pHYK/rev (SEQ ID NO:75).PHYK/rev (SEQ IDNO:75) is the plasmid of a long 5419bp, and it comprises HCMV MIEP (bp1-596), rev gene coding region (bp672-1073), BGH polyadenylation signal (bp1157-1385), Col E1 replication origin (bp1516-2099), neomycin resistance gene coding region (bp3552-2556) and ampicillin resistance gene coding region (bp4960-3929).
Fig. 6 A represents the synoptic diagram of pHGVSV-G (SEQ ID NO:74).PHGVSV-G (SEQ IDNO:74) is the plasmid of a long 7623bp, and it comprises HCMV MIEP (bp1-596), beta-globin includes subarea (bp714-1599), VSV-G coding region (bp1632-3312), BGH polyadenylation signal (bp3361-3589), Col E1 replication origin (bp3720-4303), neomycin resistance gene coding region (bp5756-4760), ampicillin resistance gene coding region (bp7164-6133) and F1 replication origin (bp7165-7621).
Fig. 6 B represents the synoptic diagram of plasmid pMYKEF1/env (SEQ ID NO:72).PMYKEF1/env (SEQ ID NO:72) is the plasmid of a long 7579bp, and it comprises HCMV MIEP (bp1-665), people EF1-α and includes subarea (bp668-1618), GaLV env coding region (bp1699-3701), BGH polyadenylation signal (bp3885-4118), Col E1 replication origin (bp4349-4832), neomycin resistance gene coding region (bp6290-5284) and ampicillin resistance gene coding region (bp7496-6666).
Photo shown in Figure 7 shows the relative quantity of the transfer vector RNA of the transfer vector genetic transcription from be transfected into people 293T target cell.
Two photos shown in Figure 8 show the transgenosis in people 293T target cell by CAEV (A) and MuLV (B).
Photo shown in Figure 9 shows (1,2 and 3 road) expressed in transfection 293T cell and is wrapped into capsid and discharge the relative quantity of the transfer vector RNA in (4,5 and 6 road) from the 293T packing cell.
Photo shown in Figure 10 shows by the relative quantity of the transfer vector RNA that wraps into capsid and discharge from people 293T packing cell.
Photo shown in Figure 11 show infect by the lentiviral vectors of VSV-G or GaLV false type that envelope protein forms and reverse transcription after the relative quantity of retrovirus cDNA of integration.
Photo shown in Figure 12 shows the relative quantity of the virus vector cDNA that is incorporated in the infected host cell chromosome.
Two pictures shown in Figure 13 show (A) control cells and (B) facs analysis of G1 phase-stagnation cell.
Two pictures shown in Figure 14 show that HIV-1-, CAEV-and MuLV-deutero-virus vector are to number of (A) transducer cell of division and Unseparated Cell and (B) relative transduction efficiency.
Embodiment
The present invention relates to based on the slow virus carrier system of CAEV and use described carrier polypeptide of interest is sent method into division and Unseparated Cell.
The CAEV genome
Wild-type CAEV virus has dimer rna gene group (strand, straight polarity), and this rna gene group is duplicated and is packaged in the virus particle of the spherical coating that contains nucleoprotein core by the double-stranded DNA intermediate.Genome comprises three genes of coding structure and zymoprotein Gag, Pol and Env and the long terminal repeat (LTR) at integrated viral genome group two ends.In addition, genome encoding is regulated albumen vif, tat and rev for three kinds.
Gag genes encoding immanent structure albumen, pol genes encoding rdrp virus, env genes encoding mediation virus is attached to the envelope glycoprotein of cell surface.Vif albumen is relevant with viral infection, and Tat albumen is relevant with the trans-activation of 5 ' LTR.The adjusting of Rev albumen and target sequence RRE thereof (Rev response element) and viral RNA stability, viral RNA montage and big RNA (montage with (singlyspliced) the sub-thread montage) are relevant from the transhipment of examining matter.Provirus LTR sequence comprises U3 (being positioned at the unique sequences element in structural protein downstream), R (the short weight complex sequencess at genome two ends) and U5 (following R sequence unique sequences element afterwards closely) district.The U3 district of 5 ' LTR comprises viral promotors and enhanser.Genomic 3 ' end comprises polyadenylation signal in 3 ' LTR.
The wild type gene group of CAEV also comprises multiple cis-activating element, comprises the atts (attachment site) that is used for provirus and is incorporated into the LTR end; Integrate the promoter element of provirus transcription initiation in 5 ' LTR control; Be positioned at the PBS (primer binding site) in 5 ' LTR downstream; 5 '-donor splicing site; Packaging sequence (herein can exchange) with packaging site or packaging signal; Ppt (polypurine tract) site of contiguous 3 ' LTR and the polyadenylation signal that is positioned at 3 ' LTR.
As used herein, term " cis " (" cis ") is used to represent that gene is present on the same karyomit(e) or the linear part of nucleic acid.Therefore, term " cis defective " is illustrated in the defective of finding on the nucleic acid linear order.Term " cis-activating " is used to represent that regulatory gene is to being present on the same karyomit(e) or the control action kou of gene on the nucleic acid linear portion.For example, influencing downstream mRNA synthetic promotor is exactly the cis acting controlling elements.
The whole genome sequence of two kinds of strain isolateds of known CAEV, and sequence is kept at (the NationalCenter for Biotechnology Information of NCBI with NC_001463 (SEQ ID NO:1) and AF322109 (SEQ ID NO:2), NCBI) (be respectively Saltarelli et al. in the database, 1990 and Gjerset, B.J.et al. does not deliver).Nucleic acid of the present invention is not limited to the concrete strain isolated of CAEV, but is limited to the sequence that keeps the genome sequence known function.For example, the natural variation of virus replication device possibility producer sequence known in the art, the similar nucleotide sequence of generation encoding function similar protein.
The sequence alignment of NC_001463 (SEQ ID NO:1) and AF322109 (SEQ ID NO:2) genome sequence sees Table 1.As table 1 as seen, have suitable nucleic acid consistence between sequence, but the difference of nucleic acid level also is tangible.Particularly importantly the variability in the CAEVgag district of table 2 note (SEQ IDNO:3-6).In table 3-6 (SEQ ID NO:7-14), can find the sequence alignment of NC_0014635 ' LTR, pol, rev and vif gene and AF322109 corresponding gene respectively.Also known and preserved the genomic a lot of partial sequences of CAEV.As, registration number AY081139, AY101347, AY101348, AY047362, AF402668, AF402667, AF402666, AF402665, AF402664, AJ305042, AJ305041 and AJ305040 provide the sequence of gag gene in CAEV Brazil strain isolated.Registration number AF015181, L78453, L78451, L78450, L78447 and L78446 also comprise the sequence of gag gene in the multiple CAEV strain isolated.Registration number X64828 and M63106 comprise the sequence of rev gene in the multiple CAEV strain isolated.Registration number AF015182, AJ305053, K03327, L78448, L78452 and U35814 comprise the sequence of pol gene in the multiple CAEV strain isolated.Sequence alignment between the gag gene (SEQ ID NO:16,18) of visible NC_001463 gag gene (SEQ IDNOs:15,17) of table 7 and AF015181.The visible NC_001463 gag gene (SEQ ID NOs:19,25) of table 8 and from the sequence alignment between the gag gene of AF402664 (SEQ ID NOs:20,26), AF402665 (SEQ ID NOs:21,27), AF402666 (SEQID NOs:22,28), AF402667 (SEQ ID NOs:23,29), AF402668 (SEQ ID NOs:24,30).The visible NC_001463gag gene (SEQ IDNOs:31,35) of table 9 and from the sequence alignment between the gag gene of AJ305040 (SEQ ID NOs:32,36), AJ305041 (SEQ ID NOs:33,37), AJ305042 (SEQ ID NOs:34,38).The visible NC_001463gag gene (SEQ ID NOs:39,41) of table 10 and from the sequence alignment between the gag gene of AY047362 (SEQ IDNOs:40,42).The visible NC_001463gag gene (SEQID NOs:43,45) of table 11 and from the sequence alignment between the gag gene of AY081139 (SEQ ID NOs:44,46).The visible NC_001463gag gene (SEQ ID NOs:47,50) of table 12 and from the sequence alignment between the gag gene of AY101347 (SEQ ID NOs:48,51) and AY101348 (SEQ ID NOs:49,52).The visible NC_001463gag gene (SEQ ID NOs:53,59) of table 13 and from the sequence alignment between the gag gene of L78446 (SEQ ID NOs:54,60), L78447 (SEQ ID NOs:55,61), L78450 (SEQ ID NOs:56,62), L78451 (SEQ ID NOs:57,63) and L78453 (SEQ IDNOs:58,64).
By VectorNTI (Informax, USA) use following parameter and compare:
Comparison in twos: the open point penalty in gap: 15
Point penalty is extended in the gap: 6.6
Multiple sequence comparison: the open point penalty in gap: 15
Point penalty is extended in the gap: 6.6
The point penalty scope is separated in the gap: 8
The percentage consistence value of the sequence alignment of listed gag gene order above table 14 has been summed up.Table 15 has been summed up the percentage consistence that full genome is compared and gag, 5 ' LTR, pol, rev and vif district compare between NC_001463 (SEQ ID NO:1) and the AF322109 (SEQ ID NO:2).Consider the genome sequence of known two CAEV strain isolateds, also have a large amount of partial sequences, and consensus sequence can easily be told that using multiple CAEV sequence to implement the present invention will not need the over-drastic experiment from multiple CAEV strain isolated.
CAEV carrier of the present invention
Carrier of the present invention provides a kind of and does not rely on host cell examine the method for duplicating and express polynucleotide or gene in system's occurrence scope widely.This carrier mediated transfection or the infection that is called as host cell in the host cell that heterologous nucleic acids is mixed wherein infects being meant the application virion, and transfection is meant and uses exposed nucleic acid molecule.
Term " gene " expression comprises the dna sequence dna that produces polypeptide or necessary control of precursor and encoding sequence.Term " polynucleotide " or " nucleic acid molecule ", as interchangeable herein, the nucleotide multimer of expression random length such as two or more, and comprises DNA and RNA.Nucleotide can be deoxyribonucleotide, ribonucleotide, nucleotide analog (the phosphoric acid structure, base or the sugar that comprise modification) or can be incorporated into substrate in the polymer by suitable enzymes such as archaeal dna polymerase or RNA polymerase arbitrarily.Polypeptide can be coded or coded by the arbitrary portion of encoding sequence by complete encoding sequence, as long as it is active to keep the expectation of polypeptide.
Term " wild-type " expression has the gene or the gene product of the feature of from natural origin isolated this gene or gene product.Wild type gene is meant in the population gene of the most frequent appearance and therefore directly is called " normally " or " wild-type " form of this gene.On the contrary, term " modification " or " sudden change " expression when compare with wild type gene or gene product sequence and or functional character on show the gene and or the gene product (change feature) of variation.The mutant that can separating natural exists, and identify according to the fact that they have a characteristic change when comparing with wild type gene or gene product.
Must be noted that, as employed in this specification and the appended claims, unless indicate in addition in the literary composition, singulative " ", " a kind of " (a, an, the) etc. comprise corresponding plural form.Therefore, comprise that as " polynucleotide " a plurality of polynucleotides and " stem cell " comprise a plurality of cells.
As used herein, utilize the RNA viruses of reversed transcriptive enzyme in term " retrovirus " the expression replicative cycle.Reverse transcription virus gene group RNA is reversed the record enzymatic conversion and becomes double-stranded DNA.This double-stranded DNA form of virus can be incorporated in the karyomit(e) of cells infected; In case integrate, just be called " provirus ".Provirus and guides coding to produce the expression of the RNA molecule of required structural protein of reovirion and enzyme as the template of rna plymerase ii.
As used herein, term " slow virus " relates to one group of (or genus) retrovirus that can cause slow developmental disease.The included virus of this group comprises human immunodeficiency virus (HIV); Cause the myelin of sheep encephalitis (visna) or pneumonia (maedi) come off-be short of breath virus (visna-maedi), arthritis-Encephalitis virus (CAEV); Equine infectious anemia virus (EIAV); Feline immunodeficiency virus (FIV); Bovine immunodeficiency virus (BIV) and simian immunodeficiency virus (SIV).The genius morbi that these viruses cause is long and course of disease delay in latent period.Usually virus lays dormant ground infects monocyte and hugely has a liking for cell, and propagates into other cells by them.
As used herein, term " carrier (vector) " expression is with the nucleic acid molecule of polynucleotide (as DNA) fragment from a cell transfer to another cell.Term " carrier (vehicle) " sometimes can with " carrier (vector) " mutual alternative.Any type of carrier (vehicle) or carrier (vector) all are included in this range of definition.For example, carrier (vector) includes but not limited to virion, plasmid, transposon etc.
The standard technique that is used to make up carrier of the present invention is well known to a person skilled in the art, and at Sambrook et al., can find in this class reference of Molecular Cloning:A Laboratory Manual 2nd Ed. (ColdSpring Harbor, N.Y., 1989).Multiple strategy may be used to connect dna fragmentation, and character and technician that the selection of strategy depends on the dna fragmentation end are easy to make a choice.
The polyadenylic acid sequence that the present invention is fit to includes but not limited to Trobest (BGH) polyadenylation signal (Pfarr et al., 1986), SV40 distinguishes polyadenylation site (Hall et al. in early days, 1983) and SV40 late region polyadenylation site (Carswell and Alwine, 1989), beta-globin polyA and herpes simplex virus thymidine kinase polyA.
Promotor of the present invention can comprise the promotor that derives from Mammals or virus, and is enough in cell to instruct and is positioned at transcribing of sequence (promptly being connected to the sequence of promoter sequence 5 ' end) at a distance.Promoter region can also comprise the controlling elements that strengthens or suppress to transcribe.Suitable promotor includes but not limited to people or murine cytomegalovirus immediate early promoter (HCMV MIEP or MCMV MIEP), EF-1 alpha (ef-1 α) and Rous sarcoma virus long-terminal repeat promoter (pRSV).Intron sequences also can make up with promotor.Intron sequences includes but not limited to ef-1 α intron and beta-globin intron.Can also use the abduction delivering system.The example of inducible system include but not limited to moulting hormone-inductive mammalian expression system (Invitrogen, CA, USA) and Tet-On and Tet-Off gene expression system (Clontech, CA, USA).The cell or tissue specificity promoter is used in orientation expression gene order in the specific cells colony.
For auxiliary expression, carrier of the present invention randomly can comprise the enhancer sequence that is positioned at promotor or terminator sequence upstream and downstream, coding region.Carrier of the present invention also can comprise additional nucleotide sequence, as intron sequences, positioning sequence or signal sequence, is enough to make cell high-efficient and processes effectively by the expressed protein of vector nucleic acid.The example of intron sequences comprises beta-globin intron (Kim et al., 2002) and people EF-1 α intron (Kim et al., 2002).Such appended sequence is inserted in the carrier,, made it to be connected, if perhaps expectation translation or processing makes it to be connected with initial sum job sequence operability in addition with the promoter sequence operability if expectation is transcribed.As an alternative, insertion sequence can place any position of carrier.
Term " operability connection " is used for describing the connection between gene order and promotor or other adjustings or the job sequence, make the promoter sequence that connects by operability instruct transcribing of gene order, regulate the translation of sequence-directed gene order by the translation that operability connects, instruct the translation post-treatment of gene order by the job sequence of operability connection.
Term " SIN carrier " expression is from the carrier of inactivation, and this carrier has the U3 district of brachymemma in 3 ' LTR.In the reverse transcription process, duplicate at 5 ' LTR in the U3 district of brachymemma, causes losing transcriptional capability and to the interference effect of endogenesis promoter.
The packaging sequence of transfer vector is made up of following substantially: (i) non-translational region between CAEV5 ' LTR and CAEV gag-encoding sequence, and the Nucleotide 1 of CAEV gag-encoding sequence that (ii) is connected to described non-translational region 3 ' end is to X, wherein X is less than 613.In one embodiment of the invention, X is selected from 60,100,125,150,175,200,225,250,275,300,325,350,375,400,425,450,475,500,525,550,575 and 600.
In another embodiment of the invention, X is selected from:
(a) X is greater than 25 and less than 600,
(b) X is greater than 25 and less than 500,
(c) X is greater than 25 and less than 400,
(d) X is greater than 25 and less than 300,
(e) X is greater than 25 and less than 200,
(f) X is greater than 50 and less than 600,
(g) X is greater than 50 and less than 500,
(h) X is greater than 50 and less than 400,
(i) X is greater than 50 and less than 300,
(j) X is greater than 50 and less than 200,
(k) X is greater than 75 and less than 600,
(l) X is greater than 75 and less than 500,
(m) X is greater than 75 and less than 400,
(n) X is greater than 75 and less than 300,
(o) X is greater than 75 and less than 200,
(p) X is greater than 100 and less than 600,
(q) X is greater than 100 and less than 500,
(r) X is greater than 100 and less than 400,
(s) X is greater than 100 and less than 300,
(t) X is greater than 100 and less than 200,
(u) X is greater than 125 and less than 600,
(v) X is greater than 125 and less than 500,
(w) X is greater than 125 and less than 400,
(x) X is greater than 125 and less than 300,
(y) X is greater than 125 and less than 200,
(z) X is greater than 150 and less than 600,
(aa) X is greater than 150 and less than 500,
(bb) X is greater than 150 and less than 400,
(cc) X is greater than 150 and less than 300,
(dd) X is greater than 150 and less than 200,
(ee) X is greater than 200 and less than 600,
(ff) X is greater than 200 and less than 500,
(gg) X is greater than 200 and less than 400,
(hh) X is greater than 200 and less than 300,
(ii) X is greater than 200 and less than 200,
(jj) X is greater than 250 and less than 600,
(kk) X is greater than 250 and less than 500,
(ll) X greater than 250 and less than 400 and
(mm) X is greater than 250 and less than 300.
In another embodiment, X is greater than 40 and less than 613.In another embodiment, X is about 327.In an embodiment of transfer vector, the codon of initial gag translation is suddenlyd change (becoming TAG, TTG, CTG or ATT as ATG) or is lacked.The trinucleotide sequence of term " codon " expression DNA or messenger RNA(mRNA) molecule, its representative instructs specific amino acids to be attached in the polypeptide chain of growth.Transfer vector also comprises allogeneic promoter and one or more cis-activating sequence.
As used herein, term " packaging signal " or " packaging sequence " expression viral RNA is packaged near the required sequence that is positioned at the CAEV genome 5 ' LTR in viral capsid or the particle.Multiple retroviral vector utilizes the required minimum package signal of packaging virus genome (be also referred to as psi[ψ] sequence).Therefore, as used herein, term " packaging sequence ", " packaging signal ", " psi " and symbol " ψ " are packed the required non-coding sequence of CAEV RNA chain during being used to represent to form virion.
In another embodiment of the invention, transfer vector also comprises transcribes box.Term used herein " is transcribed box ", and expression contains the nucleic acid fragment or the sections of one group of specific genetic elements, typically refers to the polynucleotide that operability is connected to the expression polypeptide of interest of allogeneic promoter.Can from carrier or plasmid, remove and insert in carrier or the plasmid as single cell transcribing box.
The illustrative example of transfer vector of the present invention is shown in Fig. 3 C.Fig. 3 C represents plasmid pCAH/SINd1 (SEQ ID NO:68).PCAH/SINd1 (SEQ ID NO:68) is the plasmid of a long 4238bp, comprise HCMV MIEP promotor, CAEV5 ' LTR the R-U5 sequence area, contain PBS the residue non-translated sequence, have ATG to CAEV3 ' LTR district that 327bp fragment, RRE district and the U3 of the gag gene of TAG two point sudden change lacks.This carrier also comprises Col E1 replication origin (bp2535-3118) and kalamycin resistance gene district (bp3370-4182).Other illustrative example of transfer vector are seen Fig. 3 A-3H.
The invention provides the CAEV carrier system that comprises above-mentioned transfer vector and package carrier system.The package carrier system comprises the first and second polymerized nucleoside acid vectors sequences.The first polymerized nucleoside acid vectors sequence comprises CAEV gag-pol and RRE-encoding sequence, and second polynucleotide comprises the peplos encoding sequence.In one embodiment, the second polymerized nucleoside acid encoding non--the CAEV coating.
As described here, phrase " structure gene " presentation code wraps into the polymerized nucleoside acid sequence of capsid (as packing) desired protein with viral genome, comprises gag, pol and env.
The illustrative example of the present invention's first package carrier is shown in Fig. 2 A.Fig. 2 A represents plasmid pMGP/RRE (SEQ ID NO:77).This plasmid comprises 9446 base pairs, and comprises MCMVMIEP district, CAEV gag-pol-coding region, RRE district and Trobest (BGH) polyadenylation signal.This carrier also comprises neomycin resistance gene coding region, SV40 replication origin, Col E1 replication origin and ampicillin resistance gene district.
By being used to coating, might change the host cell scope that virus vector of the present invention can infect from the close virus of another relationship.In other words, participate in the ability that other viral capsids are packed, might enlarge the host range of CAEV carrier of the present invention by utilizing some viral envelope protein.The example of retrovirus-source env gene includes but not limited to: vesicular stomatitis virus (VSV-G), gibbon ape leukemia virus (GaLV), Rous sarcoma virus (RSV), moloney murine leukemia virus (MoMuLV), mouse mammary tumor virus (MMTV) and human immunodeficiency virus's (HIV) G-albumen.All these virus envelope proteins can both form the pseudotype virus particle with other viral genome and matrix components efficiently.As described here, term " false type " the expression nucleic acid that comprises a kind of virus but has the virion of another viral envelope protein.Usually, the host range of VSV-G or GaLV pseudotyped vector is very extensive, by the super centrifugal titre (Burns et al., 1993) that can be precipitated to high density, and still keeps high-caliber infectivity.
Other illustrative example of the present invention's second package carrier are shown in Fig. 6 A and 6B.Fig. 6 A represents pHGVSV-G (SEQ ID NO:74).PHGVSV-G (SEQ ID NO:74) is the plasmid of a long 7623bp, and it comprises HCMV MIEP, beta-globin includes subarea, VSV-G coding region, BGH polyadenylation signal, Col E1 replication origin, neomycin resistance gene coding region, ampicillin resistance gene coding region and F1 replication origin.Fig. 6 B represents plasmid pMYKEF1/env (SEQID NO:72).This plasmid has 7579bp, and it comprises that HCMV MIEP, people EF1-α include subarea, GaLV env coding region, BGH polyadenylation signal, Col E1 replication origin, neomycin resistance gene coding region and ampicillin resistance gene coding region.
In another embodiment of the invention, package carrier comprises the 3rd polynucleotide of the Rev that encodes.In cells infected, Rev is attached to the Rev-response element (RRE) of virus transcription in this, causes the characteristic sub-thread montage of duplicating virus structural protein in the late stage and transcribing of montage transcript not.Therefore, the time of Rev mediation viral gene expression is regulated.Because mammalian cell montage mechanism and mRNA from nuclear synthesising part to the transhipment coupling mutually of kytoplasm, so Rev also influences the transhipment of the virus transcription that contains RRE.
The illustrative example of the present invention's three guarantees loading body as shown in Figure 5.Fig. 5 represents plasmid pHYK/rev (SEQ ID NO:75).PHYK/rev (SEQ ID NO:75) is the plasmid of a long 5419bp, and it comprises HCMV MIEP, rev gene coding region, BGH polyadenylation signal, Col E1 replication origin, neomycin resistance gene coding region and ampicillin resistance gene coding region.
In another embodiment of the invention, package carrier comprises the 4th polynucleotide of the Vif that encodes.Incorporating Vif into can be essential for the infection of virus particle and packing, and this depends on selected package cell line.
The example of the present invention's the 4th package carrier as shown in Figure 4.PHYK/vif (SEQ ID NO:76) is the plasmid of a long 5729bp, and it comprises HCMV MIEP, vif gene coding region, BGH polyadenylation signal, Col E1 replication origin, neomycin resistance gene coding region and ampicillin resistance gene coding region.
After retrovirus DNA transfection is in cell, it can integrate or can unconformability in chromosomal DNA and transcribe, thereby produce the total length retrovirus RNA that comprises psi sequence.Under these conditions, only there is vector rna to be packaged in the viral capsid structure.These virions complete but replication defective can be used in subsequently to target cell sends retroviral vector relatively efficiently.
As used herein, term " replication defective " expression can not the complete virus of effectively duplicating thereby can not produce the infectious virus particle (as replication defect type slow virus offspring).The mutated viruses that term " rf (replication-competent) " expression wild-type virus maybe can duplicate makes the virus replication of virus can produce infectious virus particle (as the replicative lentivirus offspring).
Also can consider induction type and non-induction type packing.In induction type packing cell and package cell line, respond at least a inductor and produce the CAEV particle.In the preferred embodiment of induction type clone, inductor is Tat.In non-induction type package cell line and packing cell, producing the slow virus particle does not need inductor.
CAEV carrier sequence
Functional equivalent sequence of the present invention also comprises and keeping and the multiple CAEV genomic fragment of the basic identical function of native sequences separately.These fragments comprise specific genetic elements interested at least about 10,15 continuous nucleotides, at least about 20 continuous nucleotides, at least about 24,50,60,80,100,120,140,160,180,200,220,240,260,280,300,340,360,380 or the whole continuous nucleotides of as many as.By cutting the natural viral genome with Restriction Enzyme; By synthetic nucleotide sequence from viral genome natural nucleus glycoside acid sequence; Perhaps can obtain these fragments by round pcr.Specifically referring to (Mullis and Faloona, 1987) and (Erlich, 1989).In addition, the variant of various carrier components produces as those rite-directed mutagenesises, is also included within the method for the present invention.Following more detailed description is used for determining that the method for functional equivalent can obtain in the art.
" variant " is intended to comprise similar substantially sequence.Therefore, for nucleotide sequence or aminoacid sequence, variant comprises the sequence with the various composition functional equivalents of virus carrier system.The variant nucleotide sequence also comprises the nucleotide sequence that generates, for example generates but still keep the synthetic source of native sequences function through rite-directed mutagenesis.Usually, nucleotide sequence variant of the present invention or aminoacid sequence variant and natural nucleus glycoside acid sequence separately have at least 70%, common 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% sequence identity.
The polynucleotide that variant of the present invention comprises (as carrier) comprise by with disclosed carrier sequence (SEQ ID NOs:67-79) herein have at least 70%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% conforming sequence, substantially by sequence form or form by such sequence.
The technician understands the construct of a lot of conservative property variation generation function unanimities of disclosed nucleic acid construct.The nucleic acid of the conservative property of specific nucleic acid sequence variation presentation code unanimity or basically identical acidic amino acid sequence is not perhaps represented the basically identical sequence during encoding amino acid sequence at nucleic acid.Because the degeneracy of genetic code, the nucleic acid encoding of a large amount of function unanimities is given polypeptide arbitrarily.For example, because the degeneracy of genetic code, " the reticent replacement " (promptly not causing the replacement of the nucleotide sequence that coded polypeptide changes) is the inwardness of each nucleotide sequence of coded amino acid.Similarly, packing maybe can be packed in the aminoacid sequence of construct one or more amino acid whose " conservative amino acid replacements " and is replaced by and have highly similar different aminoacids, because this is also identified easily with the high similarity that discloses construct.For example, the equal coded amino acid arginine of codon CGU, CGC, CGA, CGG, AGA and AGG.Therefore, be appointed as arginic each position by codon, this codon becomes any described corresponding codon and does not change coded polypeptide.This nucleic acid variation is " silent variant ", is a kind of of " conservative property is modified variation ".Each nucleic acid encoding sequence is also described every kind of possible silent variant herein.The technician will know can be by each codon (except AUG, it is the unique codon of methionine(Met) normally) in the standard technique modification of nucleic acids to produce the molecule of function unanimity.Therefore, each of nucleic acid encoding " silent variant " lies in any description sequence.And, the technician will know, single amino acids or small part amino acid are (typically less than 5% in change, interpolation or the disappearance encoding sequence, more typically less than 1%) single replacement, disappearance or interpolation, be " conservative property modify variation ", wherein said variation causes amino acid to be replaced by amino acid like the chemofacies.Provide the amino acid whose conservative property substitution table of functional similarity being known in the art.But comprise the amino acid of mutual alternative each other in following six groups every group:
1) L-Ala (A), Serine (S), Threonine (T);
2) aspartic acid (D), L-glutamic acid (E);
3) l-asparagine (N), glutamine (Q);
4) arginine (R), Methionin (K);
5) Isoleucine (I), leucine (L), methionine(Met) (M), Xie Ansuan (V); With
6) phenylalanine (F), tyrosine (Y), tryptophane (W).
Also can be referring to Creighton (1984) Proteins W. H.Freeman and Company.At last, adding and do not change the active sequence of nucleic acid molecule, as the non-functional sequence, is that the conservative property of basic nucleic acid is modified.The variation that this conservative property of every kind of open sequence is replaced is a feature of the present invention.
For the used various total lengths of carrier system of the present invention or the aminoacid sequence of mature polypeptide, variant comprises by the following method from natural polypeptides deutero-those polypeptides: disappearance (so-called brachymemma) or add N-end and/or the C-end of one or more amino acid to natural polypeptides; In one or more site disappearance of natural polypeptides or add one or more amino acid; Or replace one or more amino acid in one or more site of natural polypeptides.These variants can be from for example genetic polymorphism or manual operation.These working method are being known in the art.
The technician will know most approach and all can change in given nucleic acid construct.The pcr amplification that these known methods comprise rite-directed mutagenesis, carry out with the degeneracy oligonucleotide, the cellular exposure that will contain described nucleic acid in the oligonucleotide of mutagens or radiation, chemosynthesis expectation (as be connected and/or clone to combine produce big nucleic acid) and other known technologies.Referring to (Gillam and Smith, 1979), (Roberts, Cheetham and Rees, 1987) and Sambrook, Innis, Ausbel, Berger, Needham VanDevanter and Mullis (as above-mentioned).
The variant of natural acid sequence or natural polypeptides and native sequences or natural polypeptides basically identical.Can there be the difference of few to 1 to 10 amino-acid residue in variant, as 6-10, few to 5, few to 4,3,2 or even 1 amino-acid residue.Can there be the difference of few to 1 to 30 Nucleotide in the variant of nucleotide sequence, as the difference of 6-20,5,4,3,2 even 1 nucleotide residues.
In the time of when the nucleotide sequence of continuous sections of the appointment of variant nucleotide sequence or aminoacid sequence and reference sequences or aminoacid sequence comparison and relatively, " sequence identity " is intended to be illustrated in identical Nucleotide or the amino-acid residue of finding within variant sequence and the reference sequences.Conforming method is known in the art between sequence alignment and definite sequence.For the comparison of the best between two nucleotide sequences, with respect to the reference nucleotide sequence, the continuous sections of variant nucleotide sequence can have the Nucleotide of interpolation or disappearance Nucleotide.Analogue, for the best comparison of two aminoacid sequences, with respect to reference amino acid sequence, the continuous sections of variant aminoacid sequence can have the amino-acid residue of interpolation or disappearance amino-acid residue.The continuous sections that is used for comparison reference nucleotide sequence or reference amino acid sequence comprises at least 20 continuous nucleotides or amino-acid residue, and can be 30,40,50,100 or more polynucleotide or amino-acid residue.By the distribution gap point penalty, can proofread and correct increasing sequence identity, this with comprise that in variant nucleotide sequence or aminoacid sequence the gap is relevant.
Can determine consistence per-cent between two sequences with mathematical algorithm.For example can search for the consistence per-cent that (an affine 6 gap search) determine aminoacid sequence with affine 6 gaps with Smith-Waterman homology searching algorithm, the open point penalty in its intermediate gap is 12 and to extend point penalty be 2 in the gap, and the BLOSUM matrix is 62.As an alternative, using Smith-Waterman homology searching algorithm to come the consistence per-cent of definite kernel nucleotide sequence, the open point penalty in its intermediate gap is 25 and to extend point penalty be 5 in the gap.This of sequence identity determined and can be undertaken by for example DeCypher hardware accelerator with TimeLogic Version G.Smith-Waterman homology searching algorithm is instructed in Smith and Waterman, and it incorporates this paper into by reference.As an alternative, can use the GCG Gap comparison program (Wisconsin Genetic Computing Group, Suite Version 10.1) of utilizing default parameters.GCG Gap program is used Needleman and Wunch algorithm, and comparison can use open point penalty 3 in gap and gap to extend point penalty 1 for nucleotide sequence.Another of mathematical algorithm that is used for comparison two sequences is preferred, limiting examples is Karlin and Altschul algorithm (Karlin and Altschul, 1990), and improves in Karlin and Altschul (Karlin and Altschul, 1993).This algorithm has been incorporated among the NBLAST and XBLAST program of Altschul et al. (Altschul et al., 1990).Can in the NBLAST program, scoring=100 be set, the BLAST nucleotide search is carried out in word length=12, to obtain to have the nucleotide sequence of enough sequence identities.Can in the XBLAST program, scoring=50 be set, the BLAST protein search is carried out in word length=3, to obtain to have the aminoacid sequence of enough sequence identities.Be used for the comparison purpose for obtaining the gap comparison, can utilize the described Gapped BLAST of Altschul et al. (Altschul et al., 1997).As an alternative, can iterate the dependency far away of searching between detection molecules with PSI-Blast.Referring to above-mentioned Altschul et al., (1997).When using BLAST, Gapped BLAST and PSI-Blast program, can use the default parameters of program (as XBLAST and NBLAST) separately.Referring to http://www.ncbi.nlm.nih.gov.Another limiting examples that is used for the mathematical algorithm of comparative sequences is Myers and Miller algorithm (1988) CABIOS4:11-17.This algorithm is incorporated into (2.0 editions) in the ALIGN program, and it is the part of GCG sequence alignment software package.When utilizing ALIGN program comparing amino acid sequence, can with PAM120 weight surplus table (weight residue table), gap length point penalty be 12 and the gap point penalty be 4.(Informax USA) also can be used to determine the consistence per-cent of aminoacid sequence to VectorNTI.
The technician can select expectation nucleic acid of the present invention based on the knowledge of sequence that provides and the usually relevant CAEV in this area.The life cycle of slow virus, genome structure, growth adjusting and associated molecule biology have become the focus of broad research over 10 years.The specific effect of a lot of sudden changes is known in a lot of lentiviral gene groups.In addition, the variant nucleic acid sequence of number of C AEV strain is known.And according to the general knowledge about protein and properties of nucleic acids, the technician can select to have and the active similar of disclosed nucleic acid of this paper sequence table and polypeptide or the proper sequence that is equal to.
At last, the major part of nucleic acid is modified and can be estimated in the analysis that is suitable for desirable properties by conventional triage techniques.For example, the change of coded polypeptide immunological properties can detect by suitable immune analysis.The redox of the hybridization of the variation of other character such as nucleic acid and complementary nucleic acid, coded protein or thermostability, hydrophobicity, to the susceptibility of protein cleavage or assemble tendency and all can analyze according to standard technique.
Polynucleotide interested
As understood by those skilled in the art, the nucleotide sequence of the polynucleotide interested of insertion can be any nucleotide sequence.For example, the polymerized nucleoside acid sequence can be reporter gene sequence or selectable marker gene sequence.As used herein, the reporter gene sequence is any gene order, causes producing its existence or active protein that can be monitored when it is expressed.The example of suitable reporter gene comprises the gene of galactokinase, beta-galactosidase enzymes, E.C. 2.3.1.28, β-Nei Xiananmei, green fluorescent protein, enhanced green fluorescence protein etc.As an alternative, the reporter gene sequence can be can exert an influence any gene order of gene product of cell physiological of its expression.Polymerized nucleoside acid sequence of the present invention can comprise the gene order that one or more has had one or more promotor, homing sequence or job sequence.
The selectable marker gene sequence refers to express a kind of proteinic gene order, and described proteinic existence allows optionally to increase and contains its cell.The example of selectable marker gene comprises can be given host's antibiotics resistance (as tetracycline, Totomycin, Xin Meisu, Zeocin etc.) or give host's amino acid analogue resistance or allow bacterium on other carbon sources or the gene order of growing under non-permission culture condition.
Report or selectable marker gene sequence are enough to allow identification or selection carrier in normal cell.In one embodiment of the invention, general non-existent enzyme or other protein in the reporter gene sequence codified mammalian cell, thus these enzymes or proteinic existence can be determined the existence of carrier in these cells clearly.
Transfer vector of the present invention also allows heterologous nucleic acids or polynucleotide are mixed in the virion, thereby provides amplification to contain the method for the host cells infected number of heterologous nucleic acids.Mixing of allos polynucleotide promotes duplicating and the wherein production of heterologous protein subsequently of the interior heterologous nucleic acids of virion.Heterologous protein is defined herein as a kind of protein or its fragment, and wherein host cell does not express that this is proteinic all or part of.If nucleic acid or gene order are not to be used in the virus vector wild-type of cell delivery of gene natural being present in, it is called as allos so.Term heterologous nucleic acid sequence used herein or polymerized nucleoside acid sequence are intended to represent nucleic acid molecule (preferred DNA).Polymerized nucleoside acid sequence or allos polymerized nucleoside acid sequence also can comprise the encoding sequence of expecting product, wherein expect product such as suitable biologically active proteins matter or polypeptide, immunogenicity or antigen protein or polypeptide or therapeutic activity protein or polypeptide.Polypeptide can replenish the deficiency that endogenous protein is expressed in the host cell or not exist.Such gene order can have multiple source, comprises DNA, cDNA, synthetic DNA, RNA or its combination.This gene order can comprise genome cDNA, and described genome cDNA can comprise or not comprise naturally occurring intron.And this genomic dna can together obtain with promoter sequence or polyadenylic acid sequence.Gene order of the present invention is cDNA preferably.Can obtain genomic dna or cDNA by several different methods.Genomic dna can extract and purifying from suitable cell by approach well known.As an alternative, mRNA can separate acquisition and be used for preparing cDNA by reverse transcription or additive method from cell.As an alternative, the polymerized nucleoside acid sequence can comprise and RNA sequence complementary sequence, and as the sense-rna sequence, this antisense sequences can be applied to the expression that individuality suppresses complementary polynucleotide in this individual cell.
Expression of heterologous genes can provide immunogenicity or antigen protein or polypeptide to obtain antibody response.Therefore and the antibody that produces can be collected acquisition from animal body fluid, as blood, serum or ascites.
Heterologous gene can also be the nucleic acid interested that can transcribe arbitrarily.Common foreign gene coded polypeptide.Preferably polypeptide has some treatment benefits.Polypeptide can replenish the deficiency that endogenous protein is expressed in the host cell or not exist.Polypeptide can give host cell new characteristic, as chimeric frizzled receptor.Referring to U.S.Pat.No.5,359,046.Those of ordinary skill can be determined the appropriateness of this place instruction or foreign gene operative technique well known in the art.For example, the technician will know whether the size of foreign gene is fit to wrap into capsid and whether exogenous genes products can suitably express.
The concrete heterologous protein of available is verified among the present invention, and it is not crucial.
The specific examples that can be used for these heterologous proteins of the present invention comprises dystrophin (dystrophin) (Hoffman, Brow and Kunkel, 1987), blood coagulation factor VIII (Wion et al., 1985), the cystic fibrosis spanning transduction membrane is regulated albumen (Cystic Fibrosis TransmembraneRegulator Protein, CFTR) (Anderson et al., 1991; Crawford, 1991), ornithine carbamyl transferase (OTC) (Murakami et al., 1988) and alpha1-antitrypsin (Fagerhol and Cox, 1981).
The gene of a lot of heterologous proteins of encoding is known in the art, and can clone [aforementioned Sambrook et al] from genome or cDNA library.The example of this gene comprises dystrophin gene (Lee et al., 1991), blood coagulation factor VIII gene (Toole et al., 1984), cftr gene (Rommens et al., 1989; Riordan, 1989), OTC gene (Horwich et al., 1984) and alpha1-antitrypsin gene (Lemarchand et al., 1992).
In addition, the gene of coding heterologous protein, as be used for the treatment of blood vessel hyperplasia disease such as arteriosclerotic Rb (Chang et al., 1995) and be used for the treatment of cancer (Wills et al., 1994; Clayman, 1995) and the p53 of HIV disease (Bridges and Sarvet, 1995) all can be used for the present invention.
Always do not need carrier to go encoding function, heterologous gene products, promptly it can also the encoding part gene product be used as inhibitor (Warne, Viciana and Downward, 1993 of eucaryon enzyme; Wang, 1991).
Also can expect to introduce the expression that molecule is regulated generegulation molecule in (modulate) cell by the inventive method.Term " adjusting " refers to suppress its expression when the gene overexpression, maybe improve its expression when low the expression.When cell proliferation disorders is relevant with genetic expression, can use the nucleotide sequence that disturbs genetic expression in translation skill.This strategy can utilize for example antisense nucleic acid, ribozyme or triplet reagent (triplexagents), shelters its mRNA or by block transcribing or translating of specific mRNA with ribozyme cutting by antisense nucleic acid or triplet reagent.
Antisense nucleic acid is at least a portion complementary DNA or the RNA molecule with the specific mrna molecule.In cell, antisense nucleic acid forms duplex molecule with corresponding mRNA hybridization.Because not translating, cell do not form double-stranded mRNA, so antisense nucleic acid disturbs the translation of mRNA.Preferred about 15 or the antisense scant polymer of polynucleotide more, because it is convenient to synthesize, and compare its possibility that causes problem behind the introducing target cell with bigger molecule little.Using the external translation of antisense method suppressor gene is known (Marcus-Sekura, 1988) in the art.
Antisense nucleic acid can be used for blocking the expression of mutain or dominance active gene product, as the amyloid precursor protein that accumulates in alzheimer's disease.This method also can be used for treating Huntington Chorea, heredity Parkinson's disease and other diseases.Antisense nucleic acid also can be used for suppressing the expression of toxicity related protein.
Using oligonucleotide to stop and transcribe can be by known mechanism as the triplet strategy, because oligomer twines duplex DNA, forms triple helix.Therefore, the triplet compound be can design and unique site (Maher, Wold and Dervan, 1991 on the selected gene discerned; Helene, 1991).
Ribozyme is meant to have the RNA molecule that specificity is cut other single stranded RNA abilities, and its cutting mode is similar to the DNA restriction enzyme.By changing the nucleotide sequence of these RNA of coding, can design the engineering molecule (Cech, 1988) of specificity nucleotide sequence in identification and the cutting RNA molecule.This tactful major advantage is only to make the mRNA inactivation with particular sequence.
Can expect to shift the nucleic acid that encoding human is learned the effectiveness conditioning agent.That belong to such is immunostimulant (immunopotentiating agents), comprises that coding specification is " interleukin-" nucleic acid as the various kinds of cell factor of interleukin-11 to 12.Also belong to such although Interferon, rabbit working mechanism is not necessarily identical, especially (γ-IFN), tumour necrosis factor (TNF) and granulocyte-huge are had a liking for colony-stimulating factor (GM-CSF) to gamma-interferon.Can expect to medullary cell or hugely have a liking for cell and send this nucleic acid and treat congenital enzymatic defect or immune deficiency.The nucleic acid of coding somatomedin, toxicity peptide, part, acceptor or other physiology key proteins also can be introduced specific Unseparated Cell.
Therefore, recombinant C AEV carrier system of the present invention can be used for handling the cell that HIV-infects (as the T-cell or hugely have a liking for cell) by the anti-HIV molecule.In addition, for example respiratory epithelium can be regulated the recombinant slow virus infection of the present invention of albumen (CFTR) gene with having the cystic fibrosis spanning transduction membrane, treats cystic fibrosis.
Therefore, recombinant C AEV carrier system of the present invention can be used for treating multiple human diseases.CAEV carrier system can include but not limited to by applicable specific examples in human diseases: alzheimer's disease, Parkinson's disease, the amyotrophic lateral sclerosis disease, Huntington Chorea, β-thalassemia, retinitis pigmentosa, the mucopolysaccharide disease, the alba wilt disease, the chain SCID of X-(X-linked SCID), pku, tyrosinemia (tryosinemia), hemophilia A and B, the WilsonShi disease, ldl receptor lacks, human immune deficiency and Du Shi muscular dystrophy (Duchenne ' s dystrophy).
The CAEV carrier granule
In a kind of method of the present invention, can be according to method disclosed herein and in conjunction with the CAEV carrier granule that well known to a person skilled in the art technology preparation infectivity and replication defective.Described method comprises with vector expression system transfection slow virus permission cell of the present invention; In transfectional cell, produce the CAEV derivatized particles; With from cell, collect virion.
Term " transfection " expression is incorporated into foreign DNA in the eukaryotic cell as used herein.Can carry out transfection by the multiple known way in this area, include but not limited to that the transfection of calcium phosphate-DNA coprecipitation, DEAE-dextran-mediation, transfection, electroporation, microinjection, liposome fusion, lipofection (lipofection) and the protoplastis of polybrene-mediation merge.These technology are well known in the art.
As used herein, term " transduction " expression utilizes virus or the mode delivery of gene of retroviral particle by infecting but not transduceing.In some embodiments, transduction retroviral vector.Therefore, " transducible gene " is to infect or provirus is integrated and has been imported into the gene of cell by slow virus or carrier.In certain embodiments, the CAEV vector particles with gene transfer to " target cell " or host cell.
Infectious viral particle is produced in promotion in cell the also available routine techniques of step carries out, as standard cell lines incubation growth technology.
The also available routine techniques of step of collecting infectious viral particle carries out.For example, as known in the art, can collect infectious particles by the supernatant that collecting cell is cultivated.Randomly, if the virion that expectation can purifying be collected.The purification technique that is fit to is known to those skilled in the art.
If the technician needs, can prepare the CAEV liquid storage with carrier of the present invention and method.The method for preparing viral liquid storage is well known in the art, for example sees (Soneoka et al., 1995) and (Landau andLittman, 1992).In a kind of method of production liquid storage of the present invention, allow cell with carrier system transfection slow virus of the present invention.Cell is grown under suitable cell culture condition then, and as the above-mentioned CAEV particle of collecting from cell culture.The permission clone that is fit to includes but not limited to that human cell line 293,293T and HeLa, MC are Vero and goat clone GSM and Ch1Es.
Carrier of the present invention also can be used for preparing stable packing cell (be the cell of stably express CAEV structural protein, cell self can not produce infectious viral particle) and virus production cell (VPC).It is well known in the art preparing the method for expressing the proteic packing cell of retrovirus, and for example at the U.S.Pat.No.4 of Temin etal., 650,764 methods that propose as an example, wherein disclosed full content is incorporated into herein.Within the scope of the invention, packing cell comprises the slow virus permission host cell that contains from the CAEV nucleotide sequence of at least one CAEV package carrier of the present invention, its nucleotide sequence is the packaging signal defective, therefore make cell self can produce at least a CAEV structural protein, but can not produce the infectious virus of replication.According to currently known methods, allow host cell (as human embryo kidney (HEK) 293 or 293T cell) to prepare packing cell with the suitable CAEV nucleotide sequence transfection CAEV that provides previously.At least a CAEV structural protein can be expressed and produce to the gained packing cell therefore.But packing cell still can not production recombinant C AEV virus.Available then other nucleotide sequences are that transfer vector comes the transfection packing cell, and described transfer vector contains heterologous gene interested and suitable packaging signal.In case by the appended sequence transfection, packing cell can be used to provide the CAEV virus deposit that contains heterologous gene, but virus self does not have replication.The virus production cell (VPC) of gained thus can produce the infectious viral particle that contains heterologous gene interested.
Transgenosis and gene therapy
It is main candidate targets of gene therapy that single-gene changes a lot of human inheritance's diseases that cause.As used herein, term " gene therapy " or " transgenosis " are defined as for the medical treatment purpose gene are inserted in the cell.The known application that a lot of gene therapies are arranged especially insert by the heredity of stem cell, so they is known and has extensively been commented.Term " target " is used to represent the CAEV carrier described cell that is intended to transduce.The target cell of being used for the treatment of property transgenosis, exsomatize or body in, include but not limited to hemopoietic stem cell, lymphocyte, vascular endothelial cell, airway epithelial cell, keratinocyte, skeleton and muscle cell, liver cell, neurocyte and cancer cells.
Gene transfer technique of the present invention also can be used for illustrating the processing of peptide and the functional domain of identifying multiple proteins.In order to study, proteinic clone cDNA or genome sequence can be imported target cell in the different stripped or bodies in the cell-specific difference aspect processing and the cell fate.By making encoding sequence place strong promoter control down, can obtain the expectation albumen of a great deal of.In addition, the variation that sorting or bioactive specific residue can cause by the sudden change of discrete residue in the encoding sequence in the processing of participation protein, the cell is determined.
Gene transfer technique of the present invention also can be used for providing the control protein expression and estimates the instrument that it regulates the ability of cell incident.Some protein function such as they effects in differentiation, can be studied in tissue culture, yet some otherly need be reintroduced to system in the body at the different time of growing, with the variation of monitoring relevant nature,
Transgenosis provides the research nucleotide sequence and regulates the method for the cytokine of specific gene expression.A kind of strategy of this research is that regulatory element to be studied is fused on the reporter gene, the expression of subsequent analysis reporter gene.
Transgenosis is being understood and is also being had quite potential purposes in the treatment for morbid state provides.Its dcc gene of a lot of genetic diseasess is arranged is known and cloned.In some cases, the function of these clone genes is known.Usually above-mentioned morbid state is divided into two classes: deficiency state, enzyme normally, generally with recessive form heredity, and nonequilibrium state, relating to when having at least and regulating or structural protein, it is with dominant form heredity.For the deficiency state disease, transgenosis can be used for normal gene introduced in the affected tissue and is used for replacement therapy, and the animal model of setting up this disease with the antisense sudden change.For uneven morbid state, transgenosis is used in sets up morbid state in the model system, and then is used to make great efforts to overcome this morbid state.Method therefore of the present invention allows the treatment genetic diseases.As used herein, cause disease or make it more serious shortage or imbalance and treat morbid state by partially or completely correcting.Also can utilize the site-specific integration of nucleotide sequence to come mutagenesis or defect correcting.
Method of the present invention also can be used for neurone, neuroglia, inoblast or mesenchymal cell is transplanted or " transplantings ", and it comprises in cell that transplanting exsomatizes infects recombinant slow virus of the present invention or the body that infection goes in the central nervous system or in the chamber or under the dura mater on host's brain surface.These implantation methods are known to those skilled in the art, and are described in Neural Grafting in the Mammalian CNS, Bjorklund and Stenevi, eds. (1985).
For the disease that lacks protein, transgenosis can be introduced affected tissue with normal gene and be used for replacement therapy, and the animal model of setting up this disease with the antisense sudden change.For example, the nucleic acid that inserts coding Factor IX or IX in the CAEV particle be can be desirably in, muscle, spleen or liver cell infected.
The known application that a lot of gene therapies are arranged especially insert through the heredity of stem cell, so they is known and has been commented in a large number.As used herein, term " stem cell " includes but not limited to hemopoietic stem cell, neural stem cell, mesenchymal cell (especially muscle) stem cell and liver stem cells.Stem cell is regenerating tissues in vivo.Hemopoietic stem cell is the precursor cell that derives from the artificial blood progenitor cell.
Utilize the gene therapy of hemopoietic stem cell also to can be used for treating the genetic abnormality of lymph sample and medullary cell, these unusual common cause producing defective type protein or gene expression dose unusual.
For a lot of diseases, also have beneficial effect even introduce the normal copy or the function homologue of defective gene and produce missing gene product in a small amount.Simultaneously, the overexpression of expection gene product does not have deleterious effect.Followingly provide non exhaustive list of diseases, hemopoietic stem cell is gone in transgenosis has potential use to these diseases.These diseases generally include bone marrow disease, erythroid cells defective, metabolic trouble etc.Hematopoietic stem cell gene therapy helps treating the genetic diseases of hemocyte, as α-and β-thalassemia, sickle-cell anemia and hemophilia A and B, wherein globin gene or coagulation factor gene (as factors IX and factor X gene) defectiveness.Another good example is the treatment to seriousness severe combined immunodeficiency disease (SCIDS), and this type of patient lacks adenosine deaminase (ADA), and ADA helps to remove T and virose some by product of bone-marrow-derived lymphocyte, and these by products make the patient can not resist infection.This class patient is the ideal candidates object of receptor gene treatment, to its hemopoietic stem cell rather than resemble in the past to patient's lymphocyte introducing ADA gene.Other diseases comprises chronic granuloma, wherein bites neutrophil leucocyte and expresses deficient cell pigment b, and hugely have a liking for the Gaucher disease that glucose cerebrosidase (glucocerebrosidase) gene product unusual in the cell causes.
In addition, nerve degenerative diseases such as Parkinson's disease are noticeable targets of gene therapy, wherein by introducing GDNF (glial cell line-derived neurotrophic factor) gene (Kordower et al., 2000) to striatum and black substance.
The strategy of treatment various ways cancer is also included within the gene therapy.The CAEV carrier can carry the gene of encoding ratio such as toxin or inducer of apoptosis, and they are the specificity kill cancer cell effectively.Also can realize specificity kill tumor cell by under the condition of having only the tumor cells expression suicide gene, introducing suicide gene to the cancerization hematopoietic cell.Thereby the suicide gene product is not transformed into poisonous derivative and gives cell-lethal susceptibility by will normally there being cytotoxic drug.For example, Isocytosine deaminase is transformed into poisonous derivative 5 FU 5 fluorouracil (Mullen, Kilstrup and Blaese, 1992) with nontoxicity material 5 '-flucytosine.The tumour-specific lymphocyte can for example be sent the gene product with anti-tumor activity to tumor locus by genetic modification, sends the relevant toxicity of these gene products thereby overcome with systematicness.Strategies in gene therapy also can be used for providing resistance to the chemotherapeutic toxicity effect to medullary cell.
Gene therapy also can be used for preventing or resisting virus infection, infects as HIV and HTLV-1.For example, thus hemopoietic stem cell can infect cell resistance HIV by genetic modification.Article one, approach is by coming specificity to suppress viral gene expression with sense-rna or by destroying existing virus adjusting path.Shown with retrovirus RNA complementary sense-rna and can suppress multiple retroviral duplicating (To, Booth and Neiman, 1986), comprised HIV (Rhodes and James, 1991) and HTLV-1 (vonRuden and Gilboa, 1989).
Hematopoietic stem cell gene therapy is adaptable to be to alleviate autoimmune disease on the other hand.Therapeutic genes can encode for example B or T cell signal molecule, they can rebuild the normal apoptotic signal, cause the id reaction cell death or are eliminated.
Be used for diagnosing, to transform (as by transformant is infused into host's body again) be well known to a person skilled in the art for the isolated cells of research or gene therapy.In one embodiment of the invention, isolated cell from the object body is used the carrier transfectional cell of the present invention that contains polypeptide of interest, and cell is infused in object (as the patient) body again.
The various cellular types that transform that are fit to exsomatize are well known to a person skilled in the art.Particularly preferred cell be above-mentioned stem cell (referring to, Freshney (1994) Culture of Animal Cells for example, a Manual ofBasic Technique, third edition Wiley-Liss, New York, how reference discussion cited herein separates and culturing cell from the patient).Cultivate transformant by approach well known.Also referring to Kuchler (1977) Biochemical Methods in Cell Culture and Virology, Kuchler, R.J., Dowden, Hutchinson and Ross, Inc., and Atlas (1993) CRC Handbook ofMicrobiological Media (Parks ed) CRC press, Boca Raton, Fl.Mammal cell line often is the cells form of monolayer culture, but also can use the mammalian cell suspension culture.As an alternative, cell can be from the cell of preserving in the cell bank (as blood bank).The illustrative example of mammal cell line comprises HEC-1-B clone, VERO and Hela cell, Chinese hamster ovary (CHO) clone, W138, BHK, Cos-7 or mdck cell system (for example above-mentioned referring to Freshney).
T cell or B cell also can be used in some ex vivo gene transfer operation.The multiple technology that is used to separate T and B cell is known.The expression of surface marker molecule helps the evaluation and the purifying of these cells.
In a word, virus vector of the present invention can be used for stable transduction division or Unseparated Cell and stably express heterologous gene.Utilize this carrier system might in division or Unseparated Cell, introduce the proteinic gene that coding can influence cell physiological now.Therefore carrier of the present invention can be used for the gene therapy of morbid state or the experimental change of cell physiological.
Medicine box (kit)
Another object of the present invention provides medicine box or the drug delivery system that comprises the carrier that is used for method described herein.All use the required basic material of target retroviral particle and reagent can be assembled into (as packing cell construct or clone) in the medicine box.The composition of medicine box can the multiple formulations form provide.The composition that described one or more CAEV particle can be mixed with the composition that single pharmacy accepts or accept the pharmacy that separates with one or more medicament (as chemotherapeutics).
The composition of these medicine boxs or drug delivery system also can be dry or freeze dried form provide.When reagent or composition provide with dried forms, make its reconstruct by adding suitable solvent usually, described solvent also can be provided in the another kind of vessel assembly.Medicine box of the present invention also can contain relevant for target CAEV particulate dosage and or the specification sheets of drug administration information.Typically, medicine box of the present invention or drug delivery system comprise that also splendid attire enclosed construction bottle (vial) is used for the device of commercial distribution, for example, and the injection moulding or the blow-molded container of splendid attire expectation bottle.The no matter quantity or the type of container, medicine box also can comprise, or together pack auxiliary injection/use or place final complex composite thing to the intravital equipment of object into.This equipment can be medicator, sucker, syringe, suction pipe, tweezers, measuring spoon, eye drop device or the delivery vector of this class MA arbitrarily.
Following examples illustrate many aspects of the present invention, but never intention limits its scope.
Embodiment
Following examples are used for illustrating certain embodiments of the present invention and aspect, should not be construed as the restriction to its scope.
Following examples prove this discovery, that is: the slow virus carrier system that the present invention is based on recombinant C AEV and known slow virus system based on HIV-1 are the same effective aspect the expression.These embodiment show, the geneome RNA that the present invention is based on the carrier granule production system of CAEV is transcribed, is packed, the level of transduction, reverse transcription and integration is with suitable based on the slow virus carrier system of HIV-1, and be accepted (Naldini et al., 1996) already as gene transfer system efficiently based on the slow virus carrier system of HIV-1.
This is first to making up the report based on the CAEV carrier system of high titre, and this system is based on three plasmid co-transfection methods of minimum level, only needs to express gag-pol and env gene and rev gene randomly.
Material and method
Plasmid construction
Parental plasmid.CAEV carrier of the present invention is derived from pWTE-BM of parental plasmid and plasmid pCAEV-LTR, be that (Institut National de la Sante et de laRecherche Medicale " INSERM ", France) friendship provides by Marie doctor Suzan.The pWTE-BM plasmid contains the full-length gene group CAEV cDNA except 0.4kb Hind III fragment, and described Hind III fragment comprises the stuffer of part env, rev and U3 district and 1337 base pairs.Plasmid pCAEV-LTR comprises 0.4kb Hind III fragment (Saltarelli et al., 1990 that lack among the plasmid pWTE-BM; Saltarelli, 1993).Two kinds of carriers all can not produce wild-type virus.
CAEV gag-pol expression vector (pMGP/RRE) (SEQ ID NO:77) and pMGP/REV/RRE).PMGP/RRE (SEQ ID NO:77) plasmid is a PWTE-BM deutero-gag-pol expression plasmid (shown in Fig. 2 A).PMGP/RRE (SEQ ID NO:77) plasmid comprises the main immediate early gene promotor of strong allos MCMV (MCMV MIEP), gag-pol gene and rev response element (RRE).PMGP/RRE (SEQ ID NO:77) plasmid is also encoded as the neomycin resistance gene of microbiotic selective marker.In order to make up plasmid, by use to the standard operation scheme of several PCR and subclone step with gag-pol gene fragment (genomic 512 to 5046 Nucleotide of the CAEV) subclone of pWTE-BM to the pGL2-Basic cloning vector (Promega, WI, USA).From plasmid pMYK, downcut MCMV MIEP fragment (Kim et al., 2002), be inserted into the gag upstream region of gene then, RRE district (the genomic Nucleotide 7824 of CAEV to Nucleotide 8183 or Nucleotide 7849 to Nucleotide 8150) is inserted into pol gene downstream.PMGP/REV/RRE is another gag-pol expression plasmid (shown in 2B figure), and it comprises CAEV rev gene.In addition, the main donor splicing site of CAEV (the genomic Nucleotide 330 of CAEV is to Nucleotide 346) is inserted into MCMV promotor downstream.
Transfer vector (pCAH/SINd series).Make up the plasmid (shown in Fig. 3 A-3H) (SEQ ID NO:67-71,73,78 and 79) of pCAH/SINd series, identify the best packaging sequence of design transfer vector of the present invention.Each plasmid in this series is designed to comprise the initial of 5 ' non-translational region of different lengths and gag-coding region, so as one section one section relatively should the district in the effect of all lengths.For some security consideration, these plasmids are designed to have SIN (self-inactivation) carrier in the U3 district that lacks 3 ' LTR.For high level expression is from the vector rna of transfer vector under the trans-activating factor tat condition lacking, the U3 district of 5 ' LTR replaces to HCMV MIEP.In addition, transfer vector comprises polyadenylic acidization, rna transport, reverse transcription and integrates all required known cis-activating sequential elements.
Plasmid in the pCAH/SINd series (SEQ ID NO:67-71,73,78 and 79) is according to following structure.PCAH/SINd (the negative control carrier that lacks PBS) (SEQ ID NO:73) (Fig. 3 A) is designed to only contain 5 ' non-translated sequence (R and U5 district) (CAEV genome nucleotide 1 is to Nucleotide 163) at 5 ' LTR.PCAH/SINd0 (SEQ ID NO:67) (Fig. 3 B) is designed to contain whole 5 ' non-translational regions (CAEV genome nucleotide 1 is to Nucleotide 511).PCAH/SINd1 (SEQ ID NO:68) (Fig. 3 C) is designed to the 327bp fragment (CAEV genome nucleotide 1 is to Nucleotide 839) that contains whole 5 ' non-translational regions and do not have the gag gene of point mutation.The 612bp fragment (CAEV genome nucleotide 1 is to Nucleotide 1124) that pCAH/SINd2 (SEQ ID NO:69) (Fig. 3 D) is designed to contain whole 5 ' non-translational regions and the gag gene of point mutation is arranged.The 908bp fragment (CAEV genome nucleotide 1 is to Nucleotide 1420) that plasmid pCAH/SINd3 (SEQ ID NO:70) (Fig. 3 E) is designed to contain whole 5 ' non-translated sequences and the gag gene of point mutation is arranged.The 1198bp fragment (CAEV genome nucleotide 1 is to Nucleotide 1710) that plasmid pCAH/SINd4 (SEQ ID NO:71) (Fig. 3 F) is designed to contain whole 5 ' non-translated sequences and the gag gene of point mutation is arranged.Make up pCAH/SINd1/hlacZ (SEQ ID NO:78) (Fig. 3 G) among the pCAH/SINd1 (SEQ ID NO:68) by being inserted into by the expression cassette of HCMV MIEP and lacZ genomic constitution.Except the gag mrna length, plasmid pCAH/SINd60/hlacZ (SEQ ID NO:78) (Fig. 3 H) has identical design with pCAH/SINd1 (SEQ ID NO:68), and it includes the preceding 60bp fragment (CAEV genome nucleotide 1 is to Nucleotide 569) of the gag gene of point mutation.
CAEV vif expression vector (pHYK/vif) (SEQ ID NO:76).Known virus replication fast and efficiently needs the vif gene, with vif gene (CAEV genome nucleotide 5006 is to Nucleotide 5695) be cloned into carrier for expression of eukaryon pHYK (Kim et al., 2002) (Fig. 4) in.
CAEVrev expression vector (pHYK/rev) (SEQ ID NO:75).The rev gene is regulated viral gene expression by interacting with RRE at post-transcriptional level, and it forms (first exon is positioned at genomic Nucleotide 6012 to 6123, the second exons of CAEV at Nucleotide 8514 to 8803) by two exons.The Rev/RRE system promotes unspliced rna is transported nucleus, and be considered to slow virus duplicate essential.Full-length cDNA and subclone in the pHYK carrier (Fig. 5) by the synthetic rev gene of RT-PCR.
The peplos expression vector.Env gene expression vector system used herein is plasmid pHGVSV-G (SEQ ID NO:74) and plasmid pMYKEF1/env (SEQ ID NO:72) (Fig. 6 A and 6B).Plasmid pHGVSV-G (SEQ ID NO:74) is designed to express vesicular stomatitis virus G (VSV-G) glycoprotein and comprise have the beta-globin intron HCMV MIEP as promotor.Plasmid pMYKEF-1/env (SEQ ID NO:72) is designed to express gibbon ape leukemia virus (GaLV) envelope protein and comprise have eucaryon elongation factor-1 α intron MCMV MIEP as promotor.
Plasmid based on MuLV-and HIV-1.Carrier system in contrast, the present invention uses pMFG/lac/Zpuro and pHP/lacZ carrier, they are respectively to derive from murine leukemia virus (MuLV) (Kim et al., 1997) and 1 type human immunodeficiency virus's (HIV-1) (Naldini et al., 1996) the retroviral vector that contains lacZ.For the packaging plasmid of MuLV and HIV-1 carrier system, use pEQPAM3 (Persons et al., 1998) and pCMV Δ R8-2 respectively.The disappearance 1.3-kb BglII fragment, HIV-1 packaging plasmid pCMV Δ R8-2 and pCMV Δ R9 (Naldini et al., 1996) are identical in encoding function HIV-1 vpu gene and env gene.
The production of carrier granule
Enter the lentiviral vectors particle that the 293T cell is produced pseudotyping by three kinds of liposome-mediated transient transfections or more kinds of plasmid based on CAEV, wherein the 293T cell the day before yesterday of transfection with the per 6 hole culture dish bed boards of 5 * 105 cells.Gag-pol expression plasmid, metastasis transplanting physique grain and env-coding plasmid are implemented three plasmid co-transfections according to 1: 1: 1 mol ratio.Gag-pol expression plasmid, metastasis transplanting physique grain, env-coding plasmid and rev-expression plasmid were according to 3: 3: 3: 1 mol ratio is implemented four plasmid co-transfections.Gag-pol expression plasmid, metastasis transplanting physique grain and env-coding plasmid, rev-expression plasmid and vif-expression plasmid were according to 3: 3: 3: mol ratio was implemented five plasmid co-transfections in 1: 1.Collect the culture supernatant that contains vector particles after 48 hours, (USA)-70 ℃ of Ultralow Temperature Freezers are used or are stored in clarification immediately for Nalgene, NY with 0.45 μ M filter membrane.
External transduction
Following the carrying out of transduceing: under 8 μ g/ml polybrene existence conditions, in the 293T cell, add vector particles and continue 4 hours, add fresh culture subsequently.After 48 hours, cell is fixed in the solution of being made up of 1% formaldehyde and 0.2% glutaraldehyde, and containing 200 μ g 5-bromo-4-chloro-3-indoles-β-D-galactoside (X-Gal in 37 ℃, Promega, WI, USA), dyeing 12 hours in the solution of 4mM yellow prussiate of potash, the 4mM Tripotassium iron hexacyanide and 2mM MgCl2, carry out the Beta-Gal expression analysis afterwards.Form unit (LFU/ml) by counting locus coeruleus number as every ml LacZ and determine titre.
RT-PCR analyzes
(method USA) is extracted total RNA from culturing cell or culture supernatant for GIBCO BRL, CA by utilizing TRIzol LS reagent.(Promega, WI USA) handle total RNA and pollute to remove DNA with the DNaseI (1 unit/μ g DNA, 20 minutes, 37 ℃) of no RNase.The RQ1 DNase stop bath that adding provides with DNase I stops DNase I reaction, and (Qiagen, method Germany) reclaims RNA by using RNeasy mini test kit.By reverse transcription (RT) reaction the purified RNA reverse transcription is become cDNA (90 minutes, 37 ℃).Particularly, in the presence of MuLV reversed transcriptive enzyme, oligomerization dT primer or C-terminal specific primer and dNTP mixture, implement the RT reaction.Implement pcr amplification and come the semi-quantitative analysis template DNA with Auele Specific Primer.Particularly, in the presence of thermally-stabilised Ex Taq polysaccharase, sequence specific DNA primer and dNTP mixture, synthesize PCR product D NA from cDNA or chromosomal DNA.
The Southern hybridization analysis
(Qiagen Germany) prepares genomic dna from transduction has the control cells of the cell of false type HIV-1 or CAEV carrier granule and false transduction with DNeasy Tissue test kit.With the 10 μ g genomic dnas of BamHI and KpnI digestion from HIV-1 carrier transduction cell.With EcoRI and SspI double digestion from the cell of CAEV carrier transduction and each 10 μ g genomic dna of negative control cell.With the genomic dna of 0.7% agarose gel electrophoresis separating digesting, and transfer on the positively charged nylon membrane (Roche, Germany).The probe for preparing dig (digoxin) mark with lacZ gene-specific primer (forward primer: CTGGCGTAATAGCGAAGAGG (SEQ ID NO:65), reverse primer: AACTCGCCGCACATCTGAAC (SEQ ID NO:66)) by PCR, according to the Dig application manual carry out southern hybridization (Roche, Germany).The facs analysis of the growth-inhibiting of cell and growth-inhibiting cell
(Sigma, USA) (25 μ g/ml) handles growth-inhibiting 293T cell, afterwards with the transduction of CAEV vector particles with aphidocolin.As the positive or negative contrast, with HIV-1 carrier or the parallel transducer cell of MuLV retroviral vector.Transduceed back two days, and used at the active X-gal pair cell of beta-gal and dye.In the culture that aphidocolin is handled, all there is aphidocolin before and afterwards in transfection.
Confirm the growth-inhibiting of cell through facs analysis.The washing aphidocolin is handled or untreated control cells in PBS, in 70% ethanol, fixedly spend the night down at-20 ℃, (Sigma, USA) (Qiagen Germany) at room temperature handles 1 hour with RNAse A (100 μ g/ml) to use iodate third ingot (100 μ g/ml) afterwards.Estimate cell by facs analysis, and the per-cent of calculating cell cycle G1, S and the interim total viable cell of G2/M (Becton Dickinson, Sanjose, CA).
Embodiment 1
Production is based on the lentiviral vectors particle of CAEV
Generating replication defect type lentiviral vectors particulate with three pUC pUC transient cotransfection people 293T cells of the minimum level of CAEV gag-pol expression plasmid, CAEV env-expression plasmid and metastasis transplanting physique grain duplicates.In four pUC pUCs, add CAEV rev expression plasmid; In five pUC pUCs, add CAEV vif expression plasmid.For efficient packing, transfer vector is designed to comprise the initial of gag-encoding sequence, wherein introduces sudden change (ATG becomes TAG) to prevent the proteic expression of gag at initial ATG codon and the ATG codon position that is positioned at the downstream.RRE comprised wherein promote packaging efficiency, four-and five-pUC pUC in from vector expression rev to support producing of CAEV mRNA.In metastasis transplanting physique grain, insert the beta-galactosidase gene of inner HCMV-MIEP promoters driven as reporter gene.Replace the U3 district of 5 ' LTR with strong virus promotor HCMV MIEP, thereby allow the vector gene group not rely on tat.
Transfer vector rna transcription level.The transcriptional level of transfer vector geneome RNA is one of key factor of mediation high titre recombinant viral vector of production from packing cell.Among the present invention,, make up HCMV/CAEV heterozygosis LTR promoter systems with HCMV enhancers/promoters element in order to transcribe transfer vector RNA safely and efficiently.In order to check the transcriptional level of metastasis transplanting physique grain in pCAH/SINd (SEQ ID NO:67-71,73, the 78 and 79) series that contains heterozygosis LTR promotor, by liposome-mediated transfection, (pMGP/RRE (SEQ ID NO:77), pHYK/rev (SEQ ID NO:75), pHYK/vif (SEQ ID NO:76), pHGVSV-G (SEQ ID NO:74) or pMYKEF1/env (SEQ ID NO:72) introduce the human T-cell with packaging plasmid with each metastasis transplanting physique grain.After hatching 48 hours, the total RNA of purifying from transfectional cell, and implement reversed transcriptive enzyme-polymerase chain reaction (RT-PCR) and analyze and measure vector rna and transcribe.The PCR primer sets (RRE primer sets) of CAEV transfer vector is designed for the 348-bp PCR product in composite coding part RRE district.With another group HIV-1 transfer vector pHRlacZ (Naldini et al.1996) PCR primer sets (lacZ primer sets) is designed for the 645bp PCR product of composite coding part lacZ gene.As shown in Figure 7, CAEV transfer vector of the present invention produce with based on HIV-1 lentivirus transfer carrier quite level rna transcription this.
The formation of carrier granule and release.In order to check maturation and the formation and the release of infectious vector particles to be arranged, after liposome-mediated cotransfection pMGP/RRE in people 293T cell (SEQ ID NO:77) gag-pol expression plasmid, pHGVSV-G (SEQ ID NO:74) env expression plasmid, pHYK/rev (SEQID NO:75) rev expression plasmid, pHYK/vif (SEQ ID NO:76) vif expression plasmid and pCAH/SINd60/hlacZ (SEQ ID NO:78) metastasis transplanting physique grain, produce CAEV carrier granule (DuBridge et al., 1987).After the transfection 48 hours, collect the culture supernatant of transfectional cell, in the presence of 8 μ g/ml polybrene, be applied to and be used for infection in the fresh people 293T cell.The result prove five pUC pUCs of the present invention can produce with based on the suitable vector particles titre (Ory of slow virus carrier system (pEQPAM3, pMFG/lacZ/puro, pHGVSV-G (SEQ ID NO:74)) of MuLV, Neugeboren and Mulligan, 1996; Persons et al., 1998) (shown in Figure 8).
Embodiment 2
Rev and Vif express the effect that carrier granule is produced
Express the effect that carrier granule is produced in order to determine CAEV rev and vif regulatory gene, three pUC pUC (pCAH/SIN of parallel testing carrier granule production system (1) disappearance rev-and vif-encoding sequence, pMGP/RRE (SEQ ID NO:77), pHGVSV-G (SEQ ID NO:74) or pMYKEF1/env (SEQ ID NO:72)), four pUC pUC (pCAH/SIN of (2) disappearance vif-encoding sequence, pMGP/RRE (SEQ ID NO:77), pHGVSV-G (SEQ ID NO:74) or pMYKEF1/env (SEQ ID NO:72), pHYK/rev (SEQ ID NO:75)) and (3) contain five pUC pUC (pCAH/SIN of rev-and vif-encoding sequence simultaneously, pMGP/RRE (SEQ ID NO:77), pHGVSV-G (SEQ ID NO:74) or pMYKEF1/env (SEQ ID NO:72), pHYK/rev (SEQ ID NO:75), pHYK/vif (SEQ ID NO:76)) efficient aspect carrier granule production.Plasmid to each system of 293T cell transfecting.After the transfection second day, from transfectional cell and transfectional cell substratum, extract the RNA and the virus particle RNA of transfer vector respectively, and come together to detect transfer vector rna gene group as RT-PCR template and lacZ primer sets.
As shown in Figure 9, though the expression level of transfer vector RNA does not rely on rev and vif expression of gene (1,2 and 3 roads of Fig. 9) in the packing cell, the amount (Fig. 9,5 roads) of the amount (Fig. 9,4 roads) that when rev lacks, wraps into the transfer vector RNA of capsid when rev exists.But the carrier granule titre (Fig. 9,6 roads) that the RNA that surprisingly, wraps into capsid when existing with vif measures by RT-PCR is lower than and wraps into the carrier granule titre (Fig. 9,5 roads) of the RNA of capsid by RT-PCR mensuration when lacking with CAEV vif.These data show that CAEV rev and vif are not that carrier granule production is required, but rev is preferred for efficient carrier granule production.
It should be noted that the present invention is about the result of vif expression and inconsistent (Harmache et al., 1995 of Harmache et al. report; Harmache et al., 1996), wherein report the vif gene and be that CAEV efficiently duplicates is necessary in the goat synovial cell, and will influence the virus replication cycle the step in late period (as, RNA wraps into capsid, virion discharges from host cell).To this inconsistent a kind of feasible explanation can be that what to use in production recombinant C AEV carrier granule is people 293T cell rather than goat cell.The hypothesis that Seroude et al. proposes is supported in this explanation, i.e. the intercellular species specificity restriction of vif and virus production can be regulated the function (Seroude et al., 2002) of vif to viral infection.
Embodiment 3
The evaluation of optimum packing signal sequence
For identifying that CAEV transfer vector RNA wraps into the optimum packing signal sequence of capsid, according to following more a series of CAEVgag-of containing coding region different piece and be positioned at 5 ' LTR and the gag initiator codon between the carrier granule production efficiency of plasmid of non-translational region.With pMGP/RRE (SEQ ID NO:77) gag-pol expression plasmid, pHGVSV-G (SEQ ID NO:74) env expression plasmid, pHYK/rev (SEQID NO:75) rev expression plasmid, pHYK/vif (SEQ ID NO:76) vif expression plasmid and pCAH/SINd (SEQ ID NO:67-71,73,78 and 79) transfer vector series plasmid co-transfection people 293T cell.As negative control, lacking transfection CAEV transfer vector pCAM/lacZ (L) under the packaging plasmid condition.After the transfection second day, from the transfectional cell substratum, extract virus particle RNA, and come together to detect the serial rna gene group of CAEV transfer vector, or come together to detect HIV-1 transfer vector rna gene group with the lacZ primer sets as RT-PCR template and RRE primer sets.As shown in figure 10, from substratum with the 293T cell harvesting of the production virus of pCAH/SINd1 (SEQ ID NO:68) transfection, obtain strong PCR product signal, this shows that efficient release contains the virion of viral RNA, wherein pCAH/SINd1 comprises 5 ' complete LTR and the preceding 327bp in gag district (Figure 10,3 roads).This signal is suitable with the signal that obtains from positive control, HIV-1 carrier, and this shows the amount that wraps into the CAEV transfer vector RNA of the present invention in the capsid and this amount suitable (Figure 10,8 roads) based on the HIV-1 transfer vector.Have before the gag-coding region 612bp or longer CAEV transfer vector packaging efficiency and significantly reduce (4,5 and 6 road).When lacking the gag-encoding sequence, used transfer vector detects less than PCR product signal (Figure 10,1 and 2 roads).Negative control only transfection has transfer vector, and the transfection of positive control HIV-1 carrier has pCMV Δ R8-2, pHR '/lacZ and pHGVSV-G (SEQ ID NO:74) (Figure 10,7 and 8 roads).
In a word, only when transfer vector comprise the N-end gag-encoding sequence that is less than about 600bp and be positioned at 5 ' LTR and the gag initiator codon between whole non-translational region the time, transfer vector RNA just wraps into capsid efficiently in packing cell.These results prove that the effect of the secondary structure of RNA in the packaging signal is more even more important than the primary structure that RNA wraps in the capsid.
Embodiment 4
The pseudotyping of CAEV vector virus particle
In order to determine whether recombinant C AEV vector virus particle can be by GaLV glycoprotein and VSV-G glycoprotein pseudotyping, and GaLV expression vector, pMYKEF1/env (SEQ ID NO:72) or VSV-G expression vector, pHGVSV-G (SEQ ID NO:74) are with metastasis transplanting physique grain and packaging plasmid cotransfection people 293T cell.After the transfection 48 hours, collect the culture supernatant that contains the pseudotyped viral particle that transfectional cell discharges, be used to infect the 293T target cell after clarifying with 0.45 μ m filter membrane.Infect one day after, with genomic dna separating kit (Qiagen, HL, Germany) purified genomic dna and carry out PCR and test the provirus cDNA that detects integration.As expection, CAEV carrier (1 road among Figure 11) is by the efficient pseudotyping of VSV-G albumen, with MuLV-(3 roads among Figure 11) and suitable based on HIV-1 carrier (4 roads among Figure 11).In addition, inconsistent with the HIV-1 slow virus carrier system, CAEV carrier of the present invention is by GaLV coating success pseudotyping (2 roads among Figure 11).This ability with GaLV coating pseudotyping of CAEV carrier can provide the very big advantage of exploitation clinical grade slow virus carrier system.MuLV (transfection has pEQPAM3, pMFG/lacZ/puro and pHGVSV-G (SEQ ID NO:74)) and HIV-1 (transfection has pCMV Δ R8-2, pHR '/lacZ and pHGVSV-G (SEQ ID NO:74)) vehicle Control are shown in 3 and 4 roads respectively.
Embodiment 5
The generation of CAEV package cell line
PMGP/RRE (SEQ ID NO:77) and pHYK/rev (SEQ ID NO:75) carrier are all encoded and are used for the neo that screens at eukaryotic cell rGene.In order efficiently to screen after gag-pol and the rev expression vector cotransfection, available other antibiotics resistance gene such as bacterium gpt gene are replaced neo rGene makes up another CAEVgag-pol expression vector.As an alternative, can use the single packaging plasmid system of encoding gag, pol and rev gene.In order to determine whether to produce the 293T cell of stably express CAEV packaging protein, screening antibiotics resistance bacterium colony in selective medium.Show that from stable 293T cells produce recombinant C AEV carrier it is feasible producing the stabilising packaging clone be used to produce the CAEV carrier.
Embodiment 6
The integration of carrier cDNA in host chromosome based on CAEV
In order to detect the integration of transduction back CAEV carrier cDNA, produce the CAEV carrier granule by carry out liposome-mediated cotransfection pMGP/REV/RRE gag-pol expression plasmid, pHGVSV-G (SEQ ID NO:74) env expression plasmid and pCAH/SINd1/hlacZ (SEQ ID NO:79) metastasis transplanting physique grain to people 293T cell.As positive control, produce the HIV-1 carrier granule to people 293T cell cotransfection pCMV Δ R8.2 gag-pol expression plasmid, pHGVSV-G (SEQ ID NO:74) env expression plasmid and pHR/lacZ transfer vector.As negative control, transfection pCAH/SINd1/hlacZ (SEQ ID NO:79) metastasis transplanting physique grain only.After the transfection 48 hours, the culture supernatant of collecting every kind of transfectional cell was applied in the presence of 8 μ g/mlpolybrene and is used in the fresh people 293T cell infecting.After 48 hours, prepare the genomic dna of every kind of transducer cell, carry out the southern engram analysis after the Restriction Enzyme digestion.The lacZ probe of digoxigenin labeled detects 3.15kb BamH I-Kpn I fragment for the transfer vector based on HIV-1, and detects 1.35kb Hind IH-Ssp I fragment for transfer vector and the negative control based on CAEV.For positive control, use the 0.3ng and the 3ng Hind III-Ssp I dna fragmentation of pCAH/SINd1/hlacZ (SEQ ID NO:79) transfer vector.As shown in figure 12, the transfer vector that the present invention is based on CAEV be integrated based on the suitable level of the lentivirus transfer carrier of HIV-1.
Embodiment 7
Transgenosis is to Unseparated Cell
Handle the 293T cell with DNA synthetic inhibitor aphidocolin, place 6 well culture plates, transduce with the CAEV carrier granule of coding lacZ marker gene then.In contrast, with the MuLV lentiviral vectors and the parallel cells infected of HIV-1 lentiviral vectors of expressing lacZ.Infected back 48 hours, in order to detect transduction efficiency, by X-gal dyeing counting transduction lacZ expression of gene.As shown in figure 14, MuLV deutero-carrier high-efficiency infects the cell of not using the DNA inhibitor treatment.But, when cell when the DNA inhibitor treatment is suppressed in the cell cycle, transduction efficiency significantly descends.On the contrary, based on the carrier of CAEV can efficiently transduce non-splitted people cell and somatoblast, its transduction level is with suitable based on the carrier of HIV-1.
Embodiment 8
Transduction in the body of muscle cell
Among this embodiment, CAH/SINd1/hlacZ (SEQ ID NO:79) CAEV carrier is used for transduction muscle cell in the body.In the presence of 4 μ g/ml polybrene, in mouse hind leg (Beige strain) intramuscular injection 100 μ l CAEV carriers.Put to death mouse two days later, preparation is used for freezing microtome section and is used for the beta-galactosidase enzymes analysis through the tissue of injection.Expected results is the muscle cell of transduceing efficiently in CAH/SINd1lacZ (SEQ ID NO:79) the CAEV carrier body.
Above stated specification comprises specific embodiments and embodiment, is intended to illustrate the present invention, rather than as restriction.Do not depart from true spirit of the present invention and scope, can implement a lot of other changes and improvements.Cited herein all are open, comprise sequence, patent and the patent application of preserving in the ncbi database, incorporate in the disclosure through quoting its full content.
Reference
Altschul，S.F.，Gish，W.，Miller，W.，Myers，E.W.，and?Lipman，D.J.(1990).Basiclocal?alignment?search?tool.J?Mol?Biol215(3)，403-10.
Altschul，S.F.，Madden，T.L.，Schaffer，A.A.，Zhang，J.，Zhang，Z.，Miller，W.，andLipman，D.J.(1997).Gapped?BLAST?and?PSI-BLAST：a?new?generation?ofprotein?database?search?programs.Nucleic?Acids?Res25(17)，3389-402.
Anderson，M.P.，Rich，D.P.，Gregory，R.J.，Smith，A.E.，and?Welsh，M.J.(1991).Generation?of?cAMP-activated?chloride?currents?by?expression?of?CFTR.Science?251(4994)，679-82.
Bridges，S.H.，and?Sarver，N.(1995).Gene?therapy?and?immune?restoration?for?HIVdisease.Lancet?345(8947)，427-32.
Bums，J.C.，Friedmann，T.，Driever，W.，Burrascano，M.，and?Yee，J.K.(1993).Vesicular?stomatitis?virns?G?glycoprotein?pseudotyped?retroviral?vectors：concentration?to?very?high?titer?and?efficient?gene?transfer?into?mammalianand?nonmammalian?cells.Proc?Natl?Acad?Sci?USA90(17)，8033-7.
Carswell，S.，and?Alwine，J.C.(1989).Efficiency?of?utilization?of?the?simian?virus?40late?polyadenylation?site：effects?of?upstream?sequences.Mol?Cell?Biol?9(10)，4248-58.
Cech，T.R.(1988).Ribozymes?and?their?medical?implications.Jama?260(20)，3030-4.
Chang，M.W.，Barr，E.，Seltzer，J.，Jiang，Y.Q.，Nabel，G.J.，Nabel，E.G.，Parmacek，M.S.，and?Leiden，J.M.(1995).Cytostatic?gene?therapy?for?vascularproliferative?disorders?with?a?constitutively?active?form?of?the?retinoblastomagene?product.Science?267(5197)，518-22.
Curran，M.A.，and?Nolan，G.P.(2002).Nonprimate?lentiviral?vectors.Curr?TopMicrobiol?Immunol261，75-105.
Crawford，I.，Maloney，P.C.，Zeitlin，P.L，Guggino，W.B.，Hyde，S.C.，Turley，H.，Gatter，K.C.，Harris，A.，and?Higgins，C.F.(1991).Immunocytochemicallocalization?of?the?cystic?fibrosis?gene?product?CFTR.Proc?Natl?Acad?Sci?USA88(20)，9262-6.
DuBridge，R.B.，Tang，P.，Hsia，H.C.，Leong，P.M.，Miller，J.H.，and?Calos，M.P.(1987).Analysis?of?mutation?in?human?cells?by?using?an?Epstein-Barr?virusshuttle?system.Mol?Cell?Biol?7(1)，379-87.
Erlich，H.A.(1989).Polymerase?chain?reaction.J?Clin?Immunol?9(6)，437-47.Fagerhol，M.K.，and?Cox，D.W.(1981).The?Pi?polymorphism：genetic，biochemical，and?clinical?aspects?of?human?alpha?1-antitrypsin.Adv?Hum?Genet?11，1-62，371-2.
Gilbert，J.R.，and?Wong-Staal，F.(2001).HIV-2and?SIV?vector?systems.Somat?CellMol?Genet26(1-6)，83-98.
Gillam，S.，and?Smith，M.(1979).Site-specific?mutagenesis?using?syntheticoligodeoxyribonucleotide?primers：I.Optimum?conditions?and?minimumologodeoxyribonucleotide?length.Gene8(1)，81-97.
Hall，C.V.，Jacob，P.E.，Ringold，G.M.，and?Lee，F.(1983).Expression?andregulation?of?Escherichia?coli?lacZ?gene?fusions?in?mammalian?cells.JMolAppl?Genet2(1)，101-9.
Harmache，A.，Bouyac，M.，Audoly，G.，Hieblot，C.，Peveri，P.，Vigne，R.，and?Suzan，M.(1995).The?vif?gene?is?essential?for?efficient?replication?of?caprinearthritis?encephalitis?virus?in?goat?synovial?membrane?cells?and?affects?thelate?steps?of?the?virus?replication?cycle.J?Virol?69(6)，3247-57.
Harmache，A.，Russo，P.，Guiguen，F.，Vitu，C.，Vignoni，M.，Bouyac，M.，Hieblot，C.，Pepin，M.，Vigne，R.，and?Suzan，M.(1996).Requirement?of?caprinearthritis?encephalitis?virus?vif?gene?for?in?vivo?replication.Virology?224(1)，246-55.
Helene，C.(1991).The?anti-gene?strategy：control?of?gene?expression?by?triplex-forming-oligonucleotides.Anticancer?Drug?Des6(6)，569-84.
Hoffman，E.P.，Brown，R.H.，Jr.，and?Kunkel，L.M.(1987).Dystrophin：the?proteinproduct?of?the?Duchenne?muscular?dystrophy?locus.Cell?51(6)，919-28.
Horwich，A.L.，Fenton，W.A.，Williams，K.R.，Kalousek，F.，Kraus，J.P.，Doolittle，R.F.，Konigsberg，W.，and?Rosenberg，L.E.(1984).Structure?and?expressionof?a?complementary?DNA?for?the?nuclear?coded?precursor?of?humanmitochondrial?omithine?transcarbamylase.Science224(4653)，1068-74.
Karlin，S.，and?Altschul，S.F.(1990).Methods?for?assessing?the?statisticalsignificance?of?molecular?sequence?features?by?using?general?scoring?schemes.Proc?Natl?Acad?Sci?USA87(6)，2264-8.
Karlin，S.，and?Altschul，S.F.(1993).Applications?and?statistics?for?multiple?high-scoring?segments?in?molecular?sequences.Proc?Natl?Acad?Sci?USA90(12)，5873-7.
Kim，S.J.，Sadelain，M.，Choi，K.H.，Kim，H.K.，Lee，J.S.，and?Chung，H.Y.(1997).Tetracycline-mediated?suppression?of?gene?expression?with?a?newdicistronic?retroviral?vector.Mol?Cells7(4)，514-20.
Kim，S.Y.，Lee，J.H.，Shin，H.S.，Kang，H.J.，and?Kim，Y.S.(2002).The?humanelongation?factor?1alpha(EF-1?alpha)first?intron?highly?enhances?expressionof?foreign?genes?from?the?murine?cytomegalovirus?promoter.J?Biotechnol93(2)，183-7.
Kordower，J.H.，Emborg，M.E.，Bloch，J.，Ma，S.Y.，Chu，Y.，Leventhal，L.，McBride，J.，Chen，E.Y.，Palfi，S.，Roitberg，B.Z.，Brown，W.D.，Holden，J.E.，Pyzalski，R.，Taylor，M.D.，Carvey，P.，Ling，Z.，Trono，D.，Hantraye，P.，Deglon，N.，and?Aebischer，P.(2000).Neurodegeneration?prevented?bylentiviral?vector?delivery?of?GDNF?in?primate?models?of?Parkinson’s?disease.Science?290(5492)，767-73.
Landau，N.R.，and?Littman，D.R.(1992).Packaging?system?for?rapid?production?ofmurine?leukemia?virus?vectors?with?variable?tropism.J?Virol66(8)，5110-3.
Lee，C.C.，Pearlman，J.A.，Chamberlain，J.S.，and?Caskey，C.T.(1991).Expressionof?recombinant?dystrophin?and?its?localization?to?the?cell?membrane.Nature349(6307)，334-6.
Lemarchand，P.，Jaffe，H.A.，Danel，C.，Cid，M.C.，Kleinman，H.K.，Stratford-Perricaudet，L.D.，Perricaudet，M.，Pavirani，A.，Lecocq，J.P.，and?Crystal，R.G.(1992).Adenovirus-mediated?transfer?of?a?recombinant?human?alpha?1-antitrypsin?cDNA?to?human?endothelial?cells.Proc?Natl?Acad?Sci?USA89(14)，6482-6.
Maher，L.J.，3rd，Wold，B.，and?Dervan，P.B.(1991).Oligonucleotide-directedDNA?triple-helix?formation：an?approach?to?artificial?repressors？AntisenseRes?Dev?1(3)，277-81.
Marcus-Sekura，C.J.(1988).Techniques?for?using?antisenseoligodeoxyribonucleotides?to?study?gene?expression.Anal?Biochem?172(2)，289-95.
Miller，A.D.(1992).Human?gene?therapy?comes?of?age.Nature?357(6378)，455-60.
Mitrophanous，K.，Yoon，S.，Rohll，J.，Patil，D.，Wilkes，F.，Kim，V.，Kingsman，S.，Kingsman，A.，and?Mazarakis，N.(1999).Stable?gene?transfer?to?the?nervoussystem?using?a?non-Primate?lentiviral?vector.Gene?Ther6(11)，1808-18.
Mselli-Lakhal，L.，Favier，C.，Da?Silva?Teixeira，M.F.，Chettab，K.，Legras，C.，Ronfort，C.，Verdier，G.，Mornex，J.F.，and?Chebloune，Y.(1998).DefectiveRNA?packaging?is?responsible?for?low?transduction?efficiency?of?CAEV-based?vectors.Arch?Virol?143(4)，681-95.
Mullen，C.A.，Kilstrup，M.，and?Blaese，R.M.(1992).Trahsfer?of?the?bacterial?genefor?cytosine?deaminase?to?mammalian?cells?confers?lethal?sensitivity?to?5-fluorocytosine：a?negative?selection?system.Proc?Natl?Acad?Sci?USA89(1)，33-7.
Mulligan，R.C.(1993).The?basic?science?of?gene?therapy.Science?260(5110)，926-32.
Mullis，K.B.，and?Faloona，F.A.(1987).Specific?synthesis?of?DNA?in?vitro?via?apolymerase-catalyzed?chain?reaction.Methods?Enzymol?155，335-50.
Murakami，K.，Amaya，Y.，Takiguchi，M.，Ebina，Y.，and?Mori，M.(1988).Reconstitution?of?mitochondrial?protein?transport?with?purified?ornithinecarbamoyltransferase?precursor?expressed?in?Escherichia?coli.J?Biol?Chem263(34)，18437-42.
Naldini，L.，Blomer，U.，Gallay，P.，Ory，D.，Mulligan，R.，Gage，F.H.，Verma，I.M.，and?Trono，D.(1996).In?vivo?gene?delivery?and?stable?transduction?ofnondividing?cells?by?a?lentiviral?vector.Science?272(5259)，263-7.
Ory，D.S.，Neugeboren，B.A.，and?Mulligan，R.C.(1996).A?stable?human-derivedpackaging?cell?line?for?production?of?high?titer?retrovirus/vesicular?stomatitisvirus?G?pseudotypes.Proc?Natl?Acad?Sci?USA93(21)，11400-6.
Persons，D.A.，Mehaffey，M.G.，Kaleko，M.，Nienhuis，A.W.，and?Vanin，E.F.(1998).An?improved?method?for?generating?retroviral?producer?clones?forvectors?lacking?a?selectable?marker?gene.Blood?Cells?Mol?Dis24(2)，167-82.
Pfarr，D.S.，Rieser，L.A.，Woychik，R.P.，Rottman，F.M.，Rosenberg，M.，and?Reff，M.E.(1986).Differential?effects?of?polyadenylation?regions?on?geneexpression?in?mammalian?cells.DNA5(2)，115-22.
Rhodes，A.，and?James，W.(1991).Inhibition?of?heterologous?strains?of?HIV?byantisense?RNA.Aids?5(2)，145-51.
Riordan，J.R.，Rommens，J.M.，Kerem，B.，Alon，N.，Rozmahel，R.，Grzelczak，Z.，Zielenski，J.，Lok，S.，Plavsic，N.，Chou，J.L.，and?et?al.(1989).Identificationof?the?cystic?fibrosis?gene：cloning?and?characterization?of?complementaryDNA.Science?245(4922)，1066-73.
Roberts，S.，Cheetham，J.C.，and?Rees，A.R.(1987).Generation?of?an?antibody?withenhanced?affinity?and?specificity?for?its?antigen?by?protein?engineering.Nature328(6132)，731-4.
Rommens，J.M.，Iannuzzi，M.C.，Kerem，B.，Drumm，M.L.，Melmer，G.，Dean，M.，Rozmahel，R.，Cole，J.L.，Kennedy，D.，Hidaka，N.，and?et?al.(1989).
Identification?of?the?cystic?fibrosis?gene：chromosome?walking?and?jumping.Science?245(4922)，1059-65.
Saltarelli，M.，Querat，G.，Konings，D.A.，Vigne，R.，and?Clements，J.E.(1990).
Nucleotide?sequence?and?transcriptional?analysis?of?molecular?clones?ofCAEV?which?generate?infectious?virus.Virology?179(1)，347-64.
Saltarelli，M.J.，Schoborg，R.，Gdovin，S.L.，and?Clements，J.E.(1993).The?CAEVtat?gene?trans-activates?the?viral?LTR?and?is?necessary?for?efficient?viralreplication.Virology?197(1)，35-44.
Saltarelli，M.J.，Schoborg，R.，Pavlakis，G.N.，and?Clements，J.E.(1994).Identification?of?the?caprine?arthritis?encephalitis?virus?Rev?protein?and?itscis-acting?Rev-responsive?element.Virology?199(1)，47-55.
Sauter，S.L.，and?Gasmi，M.(2001).FIV?vector?systems.Somat?Cell?Mol?Genet26(1-6)，99-129.
Seroude，V.，Audoly，G.，Gluschankof，P.，and?Suzan，M.(2002).Viral?and?cellularspecificities?of?caprine?arthritis?encephalitis?virus?Vifprotein.Virology292(1)，156-61.
Smith，T.F.，Waterman，M.S.，and?Fitch，W.M.(1981).Comparative?biosequencemetrics.JMol?Evol18(1)，38-46.
Soneoka，Y.，Cannon，P.M.，Ramsdale，E.E.，Griffiths，J.C.，Romano，G.，Kingsman，S.M.，and?Kingsman，A.J.(1995).A?transient?three-plasmidexpression?system?for?the?production?of?high?titer?retroviral?vectors.NucleicAcids?Res23(4)，628-33.
To，R.Y.，Booth，S.C.，and?Neiman，P.E.(1986).Inhibition?of?retroviral?replicationby?anti-sense?RNA.Mol?Cell?Biol?6(12)，4758-62.
Toole，J.J.，Knopf，J.L.，Wozney，J.M.，Sultzman，L.A.，Buecker，J.L.，Pittman，D.D.，Kaufman，R.J.，Brown，E.，Shoemaker，C.，Orr，E.C.，and?et?al.(1984).
Molecular?cloning?of?a?cDNA?encoding?human?antihaemophilic?factor.Nature?312(5992)，342-7.
von?Ruden，T.，and?Gilboa，E.(1989).Inhibition?of?human?T-cell?leukemia?virus?tyPeI?replication?in?primary?human?T?cells?that?express?antisense?RNA.J?Virol63(2)，677-82.
Wang，C.C.(1991).A?novel?suicide?inhibitor?strategy?for?antiparasitic?drugdevelopment.J?Cell?Biochem?45(1)，49-53.
Warne，P.H.，Viciana，P.R.，and?Downward，J.(1993).Direct?interaction?of?Ras?andthe?amino-terminal?region?of?Raf-1?in?vitro.Nature?364(6435)，352-5.
Weintraub，H.M.(1990).Antisense?RNA?and?DNA.Sci?Am?262(1)，40-6.
Wills，K.N.，Maneval，D.C.，Menzel，P.，Harris，M.P.，Sutjipto，S.，Vaillancourt，M.T.，Huang，W.M.，Johnson，D.E.，Anderson，S.C.，Wen，S.F.，and?et?al.(1994).Development?and?characterization?of?recombinant?adenovirusesencoding?human?p53?for?gene?therapy?of?cancer.Hum?Gene?Ther?5(9)，1079-88.
Wion，K.L.，Kelly，D.，Summerfield，J.A.，Tuddenham，E.G.，and?Lawn，R.M.(1985).Distribution?of?factor?VIII?mRNA?and?antigen?in?human?liver?andother?tissues.Nature?317(6039)，726-9.
Table 1
PileUp
MSF：9300 Type：N Check：9398 ..
Name：NC_001463?(SEQ?ID?NO：1)?Len：9300 Check：3957 Weight：0
Name：AF322109?(SEQ?ID?NO：2) Len：9300 Check：5441 Weight：0
//
1 50
NC_001463 ..........?....GAGTTC?TAGG...AGA?GTCCCTCCTA?GTCTCTCCTC
AF322109 GTGAGTGCTC?TGAGGAGCTC?GAAGGAAAGA?GTCC.TC..A?GCCTCTCCTC
51 100
NC_001463 TCCGAGGAGG?TACCGAGACC?TCAAAATAAA?GGAGTGATTG?CCTTACTGCC
AF322109 TCCGAGGAGC?TTCGG....C?TCATAATAAA?GGAGTGCTTG?CTTCA..ACA
101 150
NC_001463 GAGTGGAGAG?TGATTACTGA?GCGGCCGGTG?TATCGGGAGT?CGTCCCTTAA
AF322109 GAACTGAG..?......CTGG?TCGTGGTTAT?TATCGGG...?.GACCGAAGT
151 200
NC_001463 TCTGTGCAAT?ACCAGAGCGG?CTCTCGCAGC?TGGCGCCCAA?CGTGGGGCCC
AF322109 CCCGTGCAAC?ACCGGGGCGG?TTCTCGCAGC?TGGCGCCCAA?CGTGGGGCTC
201 250
NC_001463 GAGGAG....?..........?..........?..........?..........
AF322109 GAGTAGCTTG?AGAAGCTCGA?CTGAGATCTG?AATCCAAGAG?CGACATCAGA
251 300
NC_001463 ....AAGAAA?AGAAAGC...?GGCCCTGAGA?ACTCGGCTTC?TG..AAAAAG
AF322109 CAGCAAGAAA?TGAGAGTAAT?GAGACCGCGA?GCTCTGCTGC?TGTAAAAAAG
301 350
NC_001463 AGGAAGAGGA?CAAGTTGCTA?TAGCAACAAG?AGAGAAGAAG?TAGAGCAAAG
AF322109 AGGAAGTAG.?CGGGTTGCCG?AGGCAACTGC?TCAGAAGAAC?CAGGGGAAAG
351 400
NC_001463 GTCCAGTGGC?T.CGGAAAAA?GAGGAACTGA?AACTTCGGGG?ACGCCTGAAG
AF322109 GGCTTCCAGC?AACCTCAAAA?GAGGAACCGA?GACTTCGGGG?ACGCCTGAA.
401 450
NC_001463 GAGTAAGGTA?AGTGACTCTG?CTGTACGCGG?GGCGAGGCAG?AGGTT.TCCT
AF322109 ..GTAAGGTA?AGTGACTCTG?CTGTACGCGG?GGCGAGGCAT?AGGAGATCCT
451 500
NC_001463 TCTAAATT.G?AAAGAGAAGT?GTTGCTGCGA?GAGGTCTTGG?TGGTCGAGAA
AF322109 TCTATTCTAG?GAAGAGAAGC?GCTGTTCTGG?GAGGTCTTGG?CGACCGAGAA
501 550
NC_001463 TCCTGTACAA?AAAAAAGGAG?GGATCTCGGT?CAGGACCAGG?ACCCCTGGGA
AF322109 TCTTGTT...?AAATAAGCCA?GGATCTCGAT?CAGGACCAAG?ACCCCTCAGG
551 600
NC_001463 GTAATACAAC?AGCAACACCG?TAAGAAAATC?CGCCATGGTG?AGTCTAGATA
AF322109 AGAGGGTATA?GACAGCGTGG?TAAGAAA.TC?CGCCGTGGTG?AGTCTAGATA
601 650
NC_001463 GAGACATGGC?GAGGCAAGTC?TCCGGGGGGA?AAAGAGATTA?TCCTGAGCTC
AF322109?GAGACATGGT?GAGGCAGGCC?TCCGGAAGGG?GAAAGGAGTA?CCCCGAGCTA
651 700
NC_001463?GAAAAATGTA?TCAAGCATGC?ATGCAAGATA?AAAGTTCGAC?TCAGAGGGGA
AF322109?AAAGAATGTC?TGAAAAAGGC?ATGCAAAATA?AAAGTAAGGG?CTGGGGGGGA
701 750
NC_001463?GCACTTGACA?GAAGGAAATT?GTTTATGGTG?CCTTAAAACA?TTAGATTACA
AF322109?GCGCCTGACA?GAAGGAAATT?GTCTCTGGTG?TATAAAAACA?CTAGAGTGTA
751 800
NC_001463?TGTTTGAGGA?CCATAAAGAG?GAACCTTGGA?CAAAAGTAAA?ATTTAGGACA
AF322109?TGTATGAGGA?TTGTAGGGAG?GAACCTTGGA?CCCCAGAAAA?ATGTAAACAA
801 850
NC_001463?ATATGGCAGA?AGGTGAAGAA?TCTAACTCCT?GAGGAGAGTA?ACAAAAAAGA
AF322109?TTATGGAAAA?AGTTGAAGCA?GGTAGAGCCT?GAGGAGAGTA?GCAAAGCAGA
851 900
NC_001463?CTTTATGTCT?TTGCAGGCCA?CATTAGCGGG?TCTAATGTGT?TGCCAAATGG
AF322109?CTATAACTCG?TTAAAAGCAA?CCTTGGCGGG?GATAGTCTGT?GTGCAAATGG
901 950
NC_001463?GGATGAGACC?TGAGACATTG?CAAGATGCAA?TGGCTACAGT?AATCATGAAA
AF322109?GAATGCAGCC?CGAGACACTG?CAGGATGCGA?TAGCAACCTT?AAACATGAGA
951 1000
NC_001463?GATGGGTTAC?TGGAACAAGA?GGAAAAGAAG?GAAGACAAAA?GAGAAAAGGA
AF322109?GA........?TGAAGTAAAA?GGAAAGGAA.?.AAGCCATCA?GAAGAAAAGA
1001 1050
NC_001463?AGAGAGTGTC?TTCCCAATAG?TAGTGCAAGC?AGCAGGAGGG?AGAAGCTGGA
AF322109?AGGGAATATA?TCCC..ATAT?TAGTGCAGGC?AGGAGGAGGA?AGAGCATGGA
1051 1100
NC_001463?AAGCAGTAGA?TTCTGTAATG?TTCCAGCAAC?TGCAAACAGT?AGCAATGCAG
AF322109?GAGCGGTAGA?GCCTGCTACC?TTTCAGCAGC?TCCAAACAGT?GGCAATGCAG
1101 1150
NC_001463?CATGGCCTCG?TGTCTGAGGA?CTTTGAAAGG?CAGTTGGCAT?ATTATGCTAC
AF322109?CATGGACTAG?TATCAGAAGA?ATTTGAAAGG?CAGCTAGCAT?ACTATGCCAC
1151 1200
NC_001463?TACCTGGACA?AGTAAAGACA?TACTAGAAGT?ATTGGCCATG?ATGCCTGGAA
AF322109?CACATGGACA?AGCAAGGATA?TCTTAGAAGT?ATTAGCCATG?ATGCCAGGAA
1201 1250
NC_001463?ATAGAGCTCA?AAAGGAGTTA?ATTCAAGGGA?AATTAAATGA?AGAAGCAGAA
AF322109?ATAGAGCGCA?AAAAGAACTA?ATACAAGGAA?AGTTAAATGA?GGAAGCAGAG
1251 1300
NC_001463?AGGTGGAGAA?GGAATAATCC?ACCACCTCCA?GCAGGAGGAG?GATTAACAGT
AF322109?AGATGGAGAA?GGCAGAATCC?ACAACCT...?...GCGGGCG?GGTTAACCGT
1301 1350
NC_001463?GGATCAAATT?ATGGGGGTAG?GACAAACAAA?TCAAGCAGCA?GCACAAGCTA
AF322109?GGATCAGATA?ATGGGGGTAG?GACAAACGAA?TCAGGCAGCG?GCACAGGCTA
1351 1400
NC_001463?ACATGGATCA?GGCAAGGCAA?ATATGCCTGC?AATGGGTAAT?AAATGCATTA
AF322109 ATATGGATCA?AGCAAGACAA?ATATGCCTAC?AATGGGTTAT?AACAGCAATA
1401 1450
NC_001463 AGAGCAGTAA?GACATATGGC?GCACAGGCCA?GGGAATCCAA?TGCTAGTAAA
AF322109 AGAGGAGTTA?GGCATATGGC?CCATAGACCA?GGAAATCCCA?TGCTGGTAAG
1451 1500
NC_001463 GCAAAAAACG?AATGAGCCAT?ATGAAGATTT?TGCAGCAAGA?CTGCTAGAAG
AF322109 ACAAAAACCA?AATGAGAACT?ATGAAGAGTT?TGCCGCAAGG?TTGTTAGAAG
1501 1550
NC_001463 CAATAGATGC?AGAGCCAGTT?ACACAGCCTA?TAAAAGATTA?TCTAAAGCTA
AF322109 CAGTGGATGC?AGAACCCGTT?ACCCAACCTA?TAAAAGAATA?TTTAAAGGTA
1551 1600
NC_001463 ACACTATCTT?ATACAAATGC?ATCAGCAGAT?TGTCAGAAGC?AAATGGATAG
AF322109 ACTCTGTCTT?ACACAAATGC?AAATTCGGAA?TGTCAAAAAC?ATATGGACAG
1601 1650
NC_001463 AACACTAGGA?CAAAGAGTAC?AACAAGCTAG?TGTAGAAGAA?AAAATGCAAG
AF322109 AGTGTTGGGG?CAAAGAGTAC?AGCAGGCCTC?AATAGAAGAA?AAAATGCAGG
1651 1700
NC_001463 CATGTAGAGA?TGTGGGATCA?GAAGGGTTCA?AAATGCAATT?GTTAGCACAA
AF322109 CATGCAGGGA?CATCGGGGGA?ACAGCATATC?AGATGCAGTT?GCTTGCACAA
1701 1750
NC_001463 GCATTAAGGC?CAGGAAAAGG?AAAAGGGAAT?GGACAGCCAC?AAAGGTGTTA
AF322109 GCCCTCCGTG?GCGGAAAAGA?AGATGGGAAA?AAATCTGTAG?GGAAGTGTTA
1751 1800
NC_001463 CAACTGTGGA?AAACCGGGAC?ATCAAGCAAG?GCAATGTAGA?CAAGGAATCA
AF322109 TAACTGTGGA?AGGCCCGGAC?ACAGAGCAAA?AGAATGCAGA?CAAGGCATTA
1801 1850
NC_001463 TATGTCACAA?CTGTGGAAAG?AGAGGACATA?TGCAAAAAGA?ATGCAGAGGA
AF322109 TATGTCACAA?CTGTGGAAAA?AGAGGGCATA?TACAGAAAAA?CTGCA....A
1851 1900
NC_001463 AAGAGAGACA?TAAGGGGAAA?ACAGCAGGGA?AACGGGAGGA?GGGGGATACG
AF322109 AC.AGAA...?.AAGAAGAAA?GGAGCAGGGA?AACATGAGGA?GGGGGCTACG
1901 1950
NC_001463 TGTGGTGCCG?TCCGCTCCTC?CTATGGAATA?ACTTCAGCAC?CACCTATGGT
AF322109 TGTGGTGCCG?TCCGCACCCC?CTATGGAGTA?ACGCAAGCAC?CACTAATAGT
1951 2000
NC_001463 TCAGGTCCGC?ATAGGTTCCC?AGCAGAGGAA?CTTGTTATTT?GATACCGGGG
AF322109 TAGGGTACAA?ATAGGGAATC?AGGAGAAACA?ATTATTATTT?GACACAGGGG
2001 2050
NC_001463 CGGACCGAAC?TATAGTTAGA?TGGCATGAGG?GCTCGGGAAA?CCCAGCCGGA
AF322109 CAGATAAAAC?GATAGTAAGA?ATGCATGATG?GAACAGGGAT?TCCAAACGGA
2051 2100
NC_001463 AGGATAAAAC?TGCAAGGAAT?AGGAGGAATA?GTAGAAGGAG?AAAAATGGAA
AF322109 AGAATAAAAT?TACAAGGGAT?AGGAGGAATA?GTAGAAGGAG?AAAAATGGAA
2101 2150
NC_001463 TAATGTAGAA?TTAGAATATA?AAGGAGAAAC?AAGAAAGGGA?ACAATAGTAG
AF322109 TAAAGTACCC?ATGACATATA?AGGGAGAAAC?ATCCTGCCCA?AGCTTGGTTG
2151 2200
NC_001463 TGTTACCACA?AAGTCCAGTA?GAAGTATTAG?GACGAGATAA?CATGGCCCGA
AF322109 TGCTAAGAGA?TAGCCCAGTA?GAAGTATTGG?GAAGAGATAA?CATGGAAGCA
2201 2250
NC_001463 TTTGGAATAA?AGATAATAAT?GGCAAATTTA?GAGGAAAAAA?GAATCCCAAT
AF322109 TTCGGCGTAA?CCCTAATAAT?GGCAAATTTA?GAAGATAAGA?AAATTCCCAC
2251 2300
NC_001463 TACAAAAGTA?AAATTGAAAG?AGGGATGTAC?GGGTCCACAT?GTCCCACAAT
AF322109 AATACCAGTA?GAATTGAAAG?AAGGATGTAA?AGGGCCACAT?GTGCCCCAGT
2301 2350
NC_001463 GGCCATTAAC?AGAAGAGAAA?TTAAAAGGTC?TAACAGAAAT?CATAGATAAA
AF322109 GGCCATTAAC?AGCAGAGAAA?TTACAAGGAC?TAACAGGAAT?AGTAGAAAAA
2351 2400
NC_001463 TTAGTGGAAG?AAGGAAAACT?AGGAAAGGCA?CCCCCACATT?GGACATGTAA
AF322109 TTACTACAGG?AAGGAAAATT?GGCAGAGGCC?CCAGAGGGAT?GGACGTGGAA
2401 2450
NC_001463 TACTCCAATC?TTTTGCATAA?AAAAGAAATC?AGGGAAGTGG?AGAATGTTAA
AF322109 CACGCCCATC?TTCTGCATAA?AAAAGAAGTC?AGGAAAATGG?AGAATGTTAA
2451 2500
NC_001463 TAGATTTCAG?AGAATTGAAC?AAACAGACAG?AAGATTTAAC?AGAAGCGCAG
AF322109 TAGATTTTAG?GGAATTAAAT?AAGCAAACAG?CAGATTTAGC?AGAAGCGCAG
2501 2550
NC_001463 TTAGGACTCC?CGCATCCGGG?AGGACTACAA?AAGAAAAAAC?ATGTTACAAT
AF322109 CTAGGACTGC?CACACCCAGG?AGGGTTGCAA?AGGAAAAAGA?ATGTAACAAT
2551 2600
NC_001463 ATTGGACATA?GGAGATGCAT?ATTTTACTAT?ACCCCTATAT?GAACCATATC
AF322109 TCTGGACATA?GGAGATGCAT?ATTTCACAAT?TCCCTTATAC?GAGCCCTATC
2601 2650
NC_001463 GAGAGTACAC?ATGTTTTACT?CTATTAAGTC?CTAATAATCT?AGGACCATGT
AF322109 AGAAATATAC?ATGCTTCACA?CTCCTAAGTC?CTAACAATTT?GGGACCATGT
2651 2700
NC_001463 AAAAGATACT?ATTGGAAAGT?GCTGCCACAA?GGTTGGAAAT?TGAGTCCATC
AF322l09 AAAAGGTATT?ATTGGAAAGT?ATTACCCCAG?GGATGGAAAT?TGAGCCCAGC
2701 2750
NC_001463 TGTATATCAA?TTTACTATGC?AGGAGATCTT?AGAGGATTGG?ATACAGCAGC
AF322109 TGTATATCAA?TTCACCATGC?AAAGGTTGTT?AAAAGGATGG?ATACAACAGC
2751 2800
NC_001463 ATCCAGAAAT?TCAATTTGGC?ATATATATGG?ATGATATTTA?CATAGGAAGT
AF322109 ATAAAAACAT?ACAATTTGGA?ATATATATGG?ATGATATCTA?TATTGGAAGT
2801 2850
NC_001463 GATTTAGAAA?TTAAAAAGCA?TAGAGAAATA?GTGAAAGATT?TAGCCAATTA
AF322109 GATCTAACGA?TAGCCCAACA?TAGGAAGATA?ATAGAAGAAT?TAGCCTCATT
2851 2900
NC_001463 TATTGCCCAA?TATGGATTCA?CTCTGCCAGA?AGAGAAGAGA?CAAAAGGGAT
AF322109 TATAGAACAA?TTTGGGTTTA?CATTACCAGA?AGATAAGAGA?CAAGAGGGCT
2901 2950
NC_001463 ATCCAGCAAA?ATGGCTAGGA?TTTGAACTAC?ACCCGCAGAC?CTGGAAATTT
AF322109 ATCCAGCAAA?ATGGCTAGGA?TTCGAGCTAC?ATCCAGAAAA?ATGGAAATAT
2951 3000
NC_001463 CAGAAGCATA?CATTACCTGA?ATTAACAAAG?GGAACAATAA?CATTAAATAA
AF322109 CAAAAGCATA?AATTGCCGGA?ATTACAAGAG?GGGGTAATAA?CCCTGAACAA
3001 3050
NC_001463 ATTACAGAAA?TTAGTAGGAG?AATTAGTATG?GAGACAATCC?ATAATTGGGA
AF322109 ATTACAGAAG?ATAGTAGGGG?AATTAGTGTG?GAGACAATCC?TTGATAGGAA
3051 3100
NC_001463 AAAGCATTCC?TAACATTCTG?AAATTAATGG?AAGGAGATAG?AGAATTACAA
AF322109 AGAGCATCCC?CAATATCATA?AAATTAATGG?AAGGAGATCG?CGCATTACAA
3101 3150
NC_001463 AGTGAAAGAA?AAATTGAAGA?AGTACATGTG?AAAGAATGGG?AAGCATGTAG
AF322109 AGTGAAAGGA?AAATAGAAAG?AATACATGTA?CAAGAATGGG?AAGCATGTCA
3151 3200
NC_001463 GAAAAAATTA?GAAGAAATGG?AAGGAAATTA?TTATAATAAA?GACAAAGATG
AF322109 AAAGAAATTA?GATGAAATGG?TAGGAAATTA?TTACAGAGAA?GAAGAAGATA
3201 3250
NC_001463 TCTATGGACA?ATTGGCTTGG?GGAGACAAAG?CTATAGAATA?TATAGTGTAT
AF322109 TCTATGGACA?AATAACTTGG?GGGGATAAGG?CAATAAAATA?CATAGTATTC
3251 3300
NC_001463 CAGGAGAAAG?GGAAACCATT?ATGGGTAAAT?GTGGTTCACA?ATATAAAGAA
AF322109 CAAAGGAAAG?GGGAACCCCT?ATGGGTAAAT?GTAGTACATG?ACATAAAAAA
3301 3350
NC_001463 CCTAAGCATC?CCGCAACAGG?TTATTAAAGC?AGCGCAAAAA?TTAACCCAAG
AF322109 TTTGAGTCTC?CCACAGCAAG?TGATAAAAGC?AGCACAGAAA?TTAACCCAGG
3351 3400
NC_001463 AAGTCATCAT?TAGGACAGGA?AAAATACCAT?GGATATTGTT?GCCAGGGAAA
AF322109 AAGTAATCAT?AAGAACAGGA?AAAATCCCAT?GGCTGCTACT?ACCAGGAAGA
3401 3450
NC_001463 GAAGAAGATT?GGAGACTAGA?ATTGCAATTA?GGGAACATCA?CATGGATGCC
AF322109 GAAGAAGACT?GGAGATTAGA?ACTGCAGGTA?GGGAACATCA?CGTGGATGCC
3451 3500
NC_00l463 AAAATTTTGG?TCCTGTTATC?GAGGA.CATA?CAAGATGGAG?AAAAAGAAAT
AF322109 ATCATTTTGG?TCATGTTATC?GAGGAGCACC?CAAG.TGGAA?AAGAAGGAAC
3501 3550
NC_001463 ATAATAGAAG?AAGTAGTAGA?AGGGCCTACA?TATTATACAG?ATGGAGGAAA
AF322109 ATAGTGGCAG?CAGTGGTAGA?TGGACCGACA?TATTATACAG?ATGGGGGAAA
3551 3600
NC_001463 AAAGAATAAA?GTAGGAAGTC?TAGGGTTCAT?AGTATCAACA?GGGGAAAAAT
AF322109 GAAAAACGCA?CAGGGAAGCT?TTGGCTTCAT?CTCCCCAACA?GGAGAAAAGT
3601 3650
NC_001463 TTAGAAAGCA?TGAAGAGGGC?ACAAACCAGC?AACTAGAATT?AAGAGCCATA
AF322109 TCAGAAGGCA?TGAAGATGGA?ACTAATCAGG?TATTAGAATT?AAGGGCAATA
3651 3700
NC_001463 GAGGAAGCTC?TAAAACAAGG?GCCTCAAACA?ATGAATTTAG?TAACAGATAG
AF322109 GAAGATCCAT?GTAAACAAGG?ACCTGAAAGC?ATGAACATTG?TAACTGACAG
3701 3750
NC_001463 TAGATATGCA?TTTGAATTTT?TATTAAGAAA?TTGGGATGAA?GAAGTAATAA
AF322109 CAGGTATGCT?TATGAATTCA?TGCTCCGAAA?CTGGGATGAA?CAGGTCATAA
3751 3800
NC_00143 AGAATCCAAT?TCAAGCAAGA?ATTATGGAAA?TTGCCCACAA?GAAAGATAGG
AF322109 GAAACCCCAT?TCAGGCAAGA?ATCATGGCAG?AAGTGCACAA?GAAAAAGCAG
3801 3850
NC_001463 ATAGGAGTGC?ATTGGGTGCC?AGGACATAAA?GGGATTCCCC?AAAATGAAGA
AF322109 GTAGGAATAC?ACTGGGTGCC?AGGGCATAAA?GGAATACCTC?AGAATGAAGA
3851 3900
NC_001463 AATAGACAAA?TATATTTCGG?AAATATTTCT?TGCAAAAGAA?GGAGAAGGAA
AF322109 GATAGACCAG?TACATATCAG?AAGTATTCTT?AGCACGAGAA?GGAACAGGGA
3901 3950
NC_001463 TTCTCCCAAA?AAGAGAAGAG?GATGCAGGGT?ATGATTTAAT?ATGCCCAGAA
AF322109 TATGTGAAAA?AAGGAAGGAA?GATGCTGGAT?ATGATTTATT?ATGCCCGCAT
3951 4000
NC_001463 GAGGTTACCA?TAGAGCCAGG?ACAAGTGAAA?TGCATCCCCA?TAGAGCTAAG
AF322109 GAGGTAATAC?TTAAACCCCA?AGAAGTAAAA?CGGATCCCAA?TAGACCTAAA
4001 4050
NC_001463 ATTAAATTTA?AAGAAATCAC?AATGGGCTAT?GATTGCTACA?AAAAGCAGCA
AF322109 ATTAAAATTG?AAAGAAAAGC?AATGGGCCAT?GATAAGTGGG?AAAAGTAGCG
4051 4100
NC_001463 TGGCTGCCAA?AGGAGTGTTC?ACACAAGGAG?GAATCATAGA?CTCAGGATAT
AF322109 TTGCAGCAAA?AGGAATATTT?GTACAAGGAG?GCATAATAGA?TTCAGGGTAT
4101 4150
NC_001463 CAGGGACAAA?TACAGGTAAT?AATGTATAAT?AGCAATAAAA?TAGCAGTAGT
AF322109 CAGGGACAAG?TACAAGTCAT?CCTATATAAT?AGTAATAAGA?TAGAGGTCAA
4151 4200
NC_001463 CATACCCCAA?GGGAGAAAAT?TTGCACAATT?AATATTAATG?GATAAAAAGC
AF322109 AATACCACAA?GGCAGGAAAT?TTGCCCAATT?AATATTAATG?AACTTACAAC
4201 4250
NC_001463 ATGGAAAATT?GGAACCCTGG?GGGGAAAGCA?GAAAAACAGA?AAGGGGAGAA
AF322109 ATGAAGAATT?AGAAGAATGG?GGAAAGGAAA?GAAAAACAGA?AAGAGGAACA
4251 4300
NC_001463 AAAGGATTTG?GGTCTACAGG?AATGTATTGG?ATAGAAAATA?TTCCTCTGGC
AF322109 AAAGGATTTG?GGTCTACAGG?AGCATTTTGG?ATAGAGAATA?TTCCCCAAGC
4301 4350
NC_001463 AGAGGAAGAC?CACACAAAAT?GGCATCAAGA?TGCCCGATCA?TTGCATCTAG
AF322109 AGAGGAAGAA?CATTACAAAT?GGCATCAAGA?TGCTAGATCT?CTGCAGCTAG
4351 4400
NC_001463 AATTTGAAAT?TCCAAGAACA?GCAGCAGAAG?ACATAGTAAA?TCAATGTGAA
AF322109 AATTCAAGAT?ACCTAGAGCA?GCAGCAGAAG?ACATTATACA?GCACTGTGAG
4401 4450
NC_001463 ATATGCAAAG?AAGCGAGGAC?ACCTGCAGTA?ATTAGAGGCG?GAAACAAAAG
AF322109 GTATGTCAAG?AAGGCAAACC?CGCAGCGATC?ACGAGAGGGG?GAAATAAAAG
4451 4500
NC_001463 GGGGGTAAAT?CATTGGCAAG?TGGATTATAC?CCATTATGAA?AATATCATAC
AF322109 AGGAATAGAT?CATTGGCAGG?TAGACTATAC?ACATTACAAA?GAACACATAA
4501 4550
NC_001463 TATTAGTATG?GGTAGAAACA?AATTCAGGAC?TAATATATGC?AGAAAAAGTA
AF322109 TATTAGTATG?GGTAGAGACT?AATTCAGGAT?TAATATTTGC?AGAGAAAGTA
4551 4600
NC_001463 AAAGGAGAAT?CAGGGCAAGA?ATTCAGAATA?AAAGTGATGC?ATTGGTATGC
AF322109 AAAGGAGAAT?CAGGACAAGA?ATTTAGGATG?CAGACATTGA?AATGGTATGC
4601 4650
NC_001463 ATTATTTGGT?CCAGAGTCAT?TGCAGTCAGA?CAATGGACCT?GCATTTGCAG
AF322109 TTTGTTTCAA?CCAAAATCAG?TGCAATCAGA?TAATGGGACA?GCCTTCACAG
4651 4700
NC_001463 CAGAGCCCAC?ACAGCTGTTA?ATGCAATACC?TAGGAGTAAA?ACACACAACA
AF322109 CTGAGGCTAC?GCAGCATCTA?ATGAAGTATT?TAGGGATTCA?GCACACTACG
4701 4750
NC_001463 GGCATACCTT?GGAATCCACA?GTCTCAGGCT?ATAGTAGAAA?GGGCACATCA
AF322109 GGTATTCCGT?GGAACCCCCA?GTCACAAAGT?TTAGTAGAAA?GAGCTCATCA
4751 4800
NC_001463 ACTATTGAAA?AGCACTTTAA?AGAAGTTCCA?GCCACAATTT?GTCGCTGTAG
AF322109 AACATTAAAA?CACATGTTAG?AAAAATTAGA?ACCACAATTT?GTGGCCCTAC
4801 4850
NC_001463 AATCAGCCAT?AGCAGCAGCC?CTAGTCGCCA?TAAATATAAA?AAGAAAGGGT
AF322109 AGTCTGCCAT?CGCAGCCACT?CTAGTTGCGC?TCAATATAAA?AAGAAAGGGT
4851 4900
NC_001463 GGGCTGGGGA?CAAGCCCTAT?GGATATTTTT?ATATATAATA?AAGAACAGAA
AF322109 GGACTAGGGG?CAAGCCCTAT?GGATATTTAC?ATATATAATA?AGGAGCAACA
4901 4950
NC_001463 AAGAATAAAT?AATAAATATA?ATAAAAATTC?TCAAAAAATT?CAATTCTGTT
AF322109 AAGACAACAA?GATAATAGTA?ATAAATTAAT?TCAGAAAA..?.AATTTTGTT
4951 5000
NC_001463 ATTACAGAAT?AAGGAAAAGA?GGACATC.AG?GAGAGTGGAA?AGGACCAACC
AF322109 ATTACAGGAT?CAGAAAAAGA?GGCCATCCAG?GAGAGTGGAA?CGGCCCAACT
5001 5050
NC_001463 CAGGTACTGT?GGAAAGGGGA?AGGAGCCAAT?TGTGGTAAAG?GATATAGAAA
AF322109 GAGGTACTGT?GGGAAGGGGA?AGGAGCCA.T?AGTAGTTAAA?GACAAAGAAA
5051 5100
NC_001463 GTGAAAAGTA?TTTAGTAATA?CCTTACAAAG?ATGCAAAATT?CATCCCGCCA
AF322109 GTGATAGATA?TCTAGTCATC?CCATATAAAG?ATGCAAAATT?TATTCCGCCA
5101 5150
NC_001463 CCAACAAAAG?AAAAGGAATA?AAAAACCTGG?ACCAGAATTA?CCCTTAGCAC
AF322109 CCGTCGGAAC?AGAAGGGATA?GAAGAATAGG?TCCAGAATTG?CCTTTATCTT
5151 5200
NC_001463 TATGGATACA?TATAGCAGAA?AGCATTAATG?GGGATAGCTC?ATGGTACATA
AF322109 TATGGACTTA?TACAGCATAC?AGCATAAATA?AAGATCCCGC?ATGGTATACA
5201 5250
NC_001463 ACAATGAGAC?TGCAACAGAT?GATGTGGGGA?AAAAGAGGAA?ATAAGTTACA
AF322109 ACCCTAAGAC?TGCAGCAAAT?GATGTGGCAT?AGGAGGGGAA?ATAAATTGAC
5251 5300
NC_001463 ATATAAGAAT?GAAGACAGGG?AATATGAAAA?TTGGGAAATT?ACATCATGGG
AF322109 ATATGTCAGG?GAAAATGCAC?AGTACGAGGA?GTGGGAAATG?ACCTCGTATG
5301 5350
NC_001463 GATGGAAAAT?GCACCTAAGG?AGAGTGAAAC?AATGGATACA?AGACAACAGG
AF322109 AGTGGAGGAT?AAGAATGAGA?AGGGACAAAA?CAAAAAGTCA?TC.CAAGAGG
5351 5400
NC_001463 AGAGGAAGC.?CCATGGCAGT?ACAAAGTAGG?AGGAACATGG?AAAAGTATAG
AF322109 GCATACTTCG?CCATGGCAAT?ATCGGAGACA?GGATGGATGG?AAGGATGTGG
5401 5450
NC_001463 GAGTGTGGTT?CCTGCAAGCA?GGAGATTACA?GAAAGGTAGA?CAGGCACTTC
AF322109 GAACGTGGTT?CCTACAGCCA?GGGGACTATA?GAAAGGCGGA?TCAGCAGTTC
5451 5500
NC_001463 TGGTGGGCAT?GGAGGATACT?GATATGTTCC?TGCAGGAAAG?AAAAGTTTGA
AF322109 TGGTTCGCTT?GGAGAATAGT?GTCGTGTTCA?TGTAAAAAGG?AAGGATTTAA
5501 5550
NC_001463 TATAAGAGAA?TTTATGAGAG?GAAGACATAG?ATGGGATTTG?TGCAAATCCT
AF322109 CATAAGAGAA?TTTATGCTAG?GTACCCATAG?ATGGGATTTG?TGTAAGTCGT
5551 5600
NC_001463 GTGCTCAAGG?AGAAGTAGTA?AAGCATACTA?GAACAAAAAG?TCTGGAAAGA
AF322109 GTTGCCAGGG?TGAAGTAGTA?AAGAGAACAC?AACCCTACAC?CTTGCAAAGG
5601 5650
NC_001463 CTAGTACTGC?TACAGATGGT?AGAACAGCAT?GTGTTTCAAG?TATTGCCATT
AF322109 CTCACGTGGC?TTAAATTAAC?AGAAGACCAT?GTATTTCAAG?TAATGCCCTT
5651 5700
NC_001463 GTGGAGAGCC?AGGAGAAGTA?GTACAACAGA?TTTCCCATGG?TGCAGGGACA
AF322109 GTGGAGAGCT?CGCAAAGGGA?TTACCATAGA?CTTTCCCTGG?TGCAGGGACA
5701 5750
NC_001463 CAACGGGATA?CACGCATGCG?TGGTCTGTCC?AGGAGTGCTG?GTTGATGGAA
AF322109 CAAAAGGATT?CCTGGAGCCG?TGGACAACGC?AAGAGTGTTG?GCAAATAGAG
5751 5800
NC_001463 TATCTCTTAG?AGGATGAGTG?AAGAACTGCC?TCAAAGAAGG?GAGACACATC
AF322109 TATCCCTTGG?AGGATGAGTG?AGGAAACCCC?AGCAGGAAGA?GAACCGACTG
5801 5850
NC_001453 CAGAAGAACT?.TGTAAGGAA?CGTACGGGAA?AGAGAAAGGG?ATACATGGCA
AF322109 CAGAGGAAAT?ATTTGAGCAA?.......GAA?GCAGAAAGT.?.....TGGAA
5851 5900
NC_001463 ATGGACAAGC?ATCAGAGTAC?CTGCGGAAAT?ACTGCAAAGA?TGGCTTGCTA
AF322109 GAGAACAAGC?GTGCGAGTCC?CAAATGACAT?ATTACAAAGA?TGGCTAGCAA
5901 5950
NC_001463 TGCTTAGGTC?AGGCAGAAAT?AGAAAGAAAG?TGTATAGAGA?AATGCAAAAA
AF322109 TGCTTAGGCA?AAGAGGAAAT?AGAAAGAAAG?TGCTTAGGGA?AATGCAAAAA
5951 6000
NC_001463 TGGATGTGGA?TACATCCCAA?GGCGCCTGTG?ATTAGGGCCT?GTGGATGCAG
AF322109 TGGGCATGGA?GGAATCCCAC?GGCGCGGGTG?ATTCGGCCGT?GTGGATGTCG
6001 6050
NC_001463 ACTATGTAAC?CCGGGGTGGG?GAACATAATC?AAGGGAATAA?TAAATGCAAA
AF322109 GCTATGTAAC?CCCGGCTGGG?GGAG.TAATT?AAT..CATAA?TAAA.GCAAA
6051 6100
NC_001463 TAAATGTAAC?TAACAAGTAG?CAAAAGTGTC?TGTGTTAGAT?GGATGCTGGG
AF322109 T...TGTAAC?..........?..........?..........?..ATGCTGTG
6101 6150
NC_001463 GCCAGATACA?TGCGCTTAAC?TGGGAAGGAA?AACTGGGTTG?AAGTAACCAT
AF322109 TC.....A..?..........?..GG......?...TGTCTTG?CAGGAA...T
6151 6200
NC_001463 GGACGGAGAG?AAGGAAAGGA?AAAGAGAAGG?TTTCACTGCG?GGACAGCAAG
AF322109 GG.CGGAGAT?AAGAAAAG..AA.GCAAAGG?AGCCACT... ..AATCCAGG
6201 6250
NC_001463 GTAAGTATCA?ACCCCAGGTA?AGTAAGCAAA?TAGGGAACAG?AAATACTAAC
AF322109 GTAAGTATAA?AAAACAGGTA?AGTA......?...G......?AA....TAAC
6251 6300
NC_001463 CCATGCTTTG?CCTATAAAGG?GATATTCCTA?TGGAGGATAT?CACTAACAAT
AF322109 ..........?....TATAGT?TATATT....?......A...?..CTAACAGT
6301 6350
NC_001463 GTGGATATTG?CTAGGGATAA?ATATGTGTGT?CAGTGCAGAG?GATTACATAA
AF322109 AAGAGCAGCA?CTAGG.....?..A.......?....GCAGAA?...TACATAA
6351 6400
NC_001463 CACTAATATC?AGATCCCTAT?GGGTTCTCAC?CCATAAAAAA?TGTGTCTGGG
AF322109 CCATAATATC?AGACCCATAT?GGGTTCTCTC?CCGTGAGAAA?TGTGTCAGGA
6401 6450
NC_001463 GTACCAGTGA?CTTGTGTAAC?AAAAGAATTC?GCAAAATGGG?GATGTCAACC
AF322109 GTACCTGTAA?CTTGTGTGAC?AAAAGAATTT?AGTAAGTGGG?GATGTCAGCC
6451 6500
NC_001463 ACTAGGAGCG?TACCCTGATC?CAGAAATAGA?ATACAGAAAT?GTGAGTCAGG
AF322109 AATAGGAGCC?TACCCAGACC?CAGACTTAGA?ATACAGAAAT?ATAAGTAAAG
6501 6550
NC_001463 AAGTAGTGAA?AGAAGTATAT?CAAGAGAATT?GGCCATGGAA?TACATATCAT
AF322109 AAATATTAGA?GGAAGTATAT?CAACAAGACT?GGCCGTGGAA?TACTTATCAT
6551 6600
NC_001463 TGGCCTCTCT?GGCAAATGGA?GAATGTTAGG?TACTGGTTAA?AAGAAAATAT
AF322109 TGGCCATTAT?GGCAAATGGA?TAATGTAGTA?CAATGGGCAA?GGCAAAATTT
6601 6650
NC_001463 GCAAGAAAAT?CAACAGAGAA?AAAATAATAC?AAAAGAGGGT?ATAGAGGAAT
AF322109 ACAGGATAAC?CGCAAG.GAA?AAAAG.....?......GGAC?CTGGCAGACC
6651 6700
NC_001463 TATTAGCAGG?AACTATAAGG?GGAAGATTCT?GTGTACCATA?CCCATTTGCC
AF322109 TATTAGCAGG?AAAAATAAGG?GGAAGATTCT?GTGTACCCTA?CCCATTTGCG
6701 6750
NC_001463 TTGTTAAAAT?GCACAAAGTG?GTGCTGGTAT?ACAGCGGCCA?TAAA..CAAC
AF322109 CTCCTGGAGT?GCATGGAATG?GTGCTGGTGG?GTTAAGAACA?CTAATGCAGG
6751 6800
NC_001463 GAGTCA.GGA?AAAGCAGGAA?AAATAAAAAT?AAATTGCACA?GAAGCAAGAG
AF322109 GGGGTATGGA?GAAGCAG..A?.CATAAGAAT?AAATTGCTCA?AGGGCAAGAG
6801 6850
NC_001463 CAGTCTCCTG?TACAGAGGAC?ATGCCATTAG?CCTCAATACA?AAGAGCATAT
AF322109 CAGTGAGCTG?CACAAGTGAA?ATGCCCTTAG?CATCCCTACA?GAGGGTATAT
6851 6900
NC_001463 TGGGATGAGA?AAGACAGAGA?GAGCATGGCC?TTTATGAATA?TCAAAGCATG
AF322109 TGGGAAAAGG?AGGAACGAAA?AAACATGGAG?AAAATGACCA?TCAAACCTTG
6901 6950
NC_001463 TGATAGCAAC?CTAAGGTGTC?AGAAAAGACC?TGGAGGGTGT?ATGGAAGGAT
AF322109 CAATAAAAAT?TTGGAATGCA?AGAACAGAA.?.G.GGGATGC?GCAGAAGGGT
6951 7000
NC_001463 ACCCTATCCC?AGTAGGAGCA?GAAATAATCC?CTGAAAGTAT?GAAATACCTA
AF322109 ATCCAGTACC?TCCCAAGGCA?GAGTTATTCC?CTCCAGCGTT?TCAGGATTTA
7001 7050
NC_001463 AGGGGAGCAA?AGAGTCAG..?TATGGGGGAA?TAAAAGATAA?GAATGGAGAA
AF322109 CAGCCA..AA?AGGGTACGCA?TATGGGGCAC?TTAGAG...G?GAACAGCAAA
7051 7100
NC_001463 TTAAAATTAC?CATTAACATT?AAGAGTGTGG?GTAAAATTAG?CAAATGTGTC
AF322109 TTTCCACAAA?GAGTGTCGCT?AAGAACATGG?GTGAAAATAG?CTAACCTGAC
7101 7150
NC_001463 AGAATGGGTA?AATGGGACAC?CCCCGGATTG?GCAAGACAGA?ATTAACGGAT
AF322109 AGGATGGGAA?AAAGGAAAGC?CAGCAGAATG?GT......GG?AATACCAG..
7151 7200
NC_001463 CCAAAGGAAT?AAATGGGACG?CTCTGGGGAG?AGCTTAACAG?TATGCATCAC
AF322109 CCAACAGGTT?CATTGGTTTG?ATACCACGCC?ACAATATCAT?TTAGGAT...
7201 7250
NC_001463 CTAGGATTTG?CCCTTAGCCA?GAACGGCAAA?TGGTGTAACT?ACACCGGGGA
AF322109 .ATGTATTAT?CCCGAGCGCC?TGAGAACAGG?AGTTGTAATT?TCACAGGGGA
7251 7300
NC_001463 AATAAAATTA?GGGCAAGAAA?CATTCCAATA?TCATTACAAG?CCAAACTGGA
AF322109 AATACGAATA?GGGCAACATC?AGTTTGAGTA?TAATTACACC?CTGACAAAGA
7301 7350
NC_001463 ACTGTACC..?.GGGAATTGG?ACGCAATATC?CGGTGTGGCA?AGTGATTAGA
AF322109 ATTGCACAAA?GGAGAAGTGG?AAAGAGTACC?CCATGTGGCA?TGTCTGGAGG
7351 7400
NC_001463 AACCTGGATA?TGGTGGAACA?TATGACAGGA?GAATGTGTGC?AGAGACCACA
AF322109?CATTTAGATC?AAAATGAGCA?CTTATCTAGC?ATATGTTTCA?AAAGACCGAG
7401 7450
NC_001463?AAGGCACAAT?ATAACAGTAG?GAAATGGAAC?CATAACAGGG?AATTGCAGTA
AF322109?AAGAAATGCA?ACACAAATAG?GGAACAGTAC?ACTGCAAGGG?CAATGTAATA
7451 7500
NC_001463?CAACAAACTG?GGATGGATGT?AATTGCTCAC?GATCAGGAAA?CTACCTATAT
AF322109?GAAGTAATTG?GACAGGATGC?CACTGCAATG?AGACAGGGAT?AAAC..AC..
7501 7550
NC_001463?AACAGCTCTG?AGGGAGGATT?GTTATTAATT?CTGTGCAGAC?AAAACAGCAC
AF322109?AACA......?..........?..........?...TGGAGAA?TAAATGGCAC
7551 7600
NC_001463?CCTAACAAGG?ATCCTGGGAA?CAAATACAAA?TTGGACAACT?ATGTGGGGAA
AF322109?....AAAGGG?AGC.TT..AT?CTCTTA..AA?TAGCACTAAT?.....GGAAA
7601 7650
NC_001463?TATACAAAAA?TTGTTCAGGA?TGCGAGAATG?CAACATTAGA?CAACACAGGA
AF322109?CATCATGGTC?TTGTT....A?TGCTGGAACA?CAACAGTGG.?.....CAGGG
7651 7700
NC_001463?GAAGGAACCT?TAGGAGGTGT?AGCTAATAAG?AACTGTAGCT?TGCCTCATAA
AF322109?GTA.......?TATGAGAGTC?AGCTAA....?A.GTGGAATG?AGAGTCTTAA
7701 7750
NC_001463?AAATGAGAGC?AACAAGTGGA?CTTGTGCCCC?AAGACAAAGA?GATGGAAAAA
AF322109?AGACGGAGAC?TATGGGCTCT?GTTTTAATTC?AACAAACAGG?AATTGTACTA
7751 7800
NC_001463?CAGATTC.GC?TATACATAGC?AGGAGGAAAA?AAGTTTTGGA?CACGAATTAA
AF322109?GAAATGGAGC?TCGGCACTAT?GTAAACAAGA?GAGTGATAAA?AAACGAC.AC
7801 7850
NC_001463?GGCCCAATTC?AGCTGTGAAA?GTAACATAGG?ACAATTAGAT?GGAATGTTGC
AF322109?AGCAGATCAT?AATTGTGATA?GCAGCATATC?AGCAATAGAT?GGAATGGTAC
7851 7900
NC_001463?ATCAGCAAAT?ACTATTGCAA?AAATATCAAG?TAATTAAGGT?AAGAGCTTAT
AF322109?ATCAACAAAT?ATTACTGCAA?AGGTATCAAG?TAATTAGAGT?AAGAGCTTAC
7901 7950
NC_001463?ACATATGGGG?TGATAGAAAT?GCCAGAAAAC?TATGCAAAAA?CAAGAATCAT
AF322109?ACATACGGAG?TGATTGATAT?GCCAGACAAT?TATG.AGACC?CTACCAGGA.
7951 8000
NC_001463?AAACAGGAAA?AAAAGAGAAC?TCAGCCACAA?GAGGAAGAAG?AGAGGCGTTG
AF322109?....AGGAGA?AGGAGAGATC?TCGCAAAGGC?CAGGAAAAAG?AGGGGCGTGG
8001 8050
NC_001463?GCTTGGTCAT?TATGCTAGTT?ATCATGGCAA?TAGTAGCTGC?CGCAGGGGCT
AF322109?GCCTGGTCAT?CATGTTAGCT?ATCATGGCCA?TAGTGGCTGC?TGCAGGAGCA
8051 8100
NC_001463?TCTCTGGGAG?TCGCAAACGC?GATTCAGCAG?TCTTACACTA?AGGCAGCTGT
AF322109?TCTCTGGGAG?TCGCGAACGC?GATTCAGCAG?TCCTACACCA?GGGACGCTGT
8101 8150
NC_001463?CCAGACCCTT?GCTAATGCAA?CTGCTGCACA?GCAGGATGTG?TTAGAGGCAA
AF322109 CCAGACTCTT?GCTAACGCGA?CTGCTGTGCA?ACAGCAGGTG?TTAGAGGCGT
8151 8200
NC_001463 CCTATGCCAT?GGTACAGCAT?GTGGCTAAAG?GCGTACGAAT?CTTGGAAGCT
AF322109 CCTATGCCAT?GATACAGCAT?GTGGCTAAGG?GAATACGCAT?CCTTGAAGCA
8201 8250
NC_001463 CGAGTGGCTC?GAGTGGAAGC?TATCACAGAT?AGAATAATGC?TATACCAAGA
AF322109 CGCGTGGCGA?GAATGGAAGT?TATGATGGAT?AGAATGATGT?TATATCAGGA
8251 8300
NC_001463 ATTGGATTGT?TGGCACTATC?ATCAATACTG?TATAACCTCT?ACAAAAACAG
AF322109 AGTAGACTGC?TGGCATTATC?ACCAATATTG?TGTAACCTCT?ACAAGAGCAG
8301 8350
NC_001463 AAGTAGCAAA?ATATATCAAT?TGGACGAGGT?TTAAGGATAA?TTGCACATGG
AF322109 ACATAGTGAA?TTACATTAAT?TGGACAAGGT?TTAAAGATAA?TTGCACATGG
8351 8400
NC_001463 CAGCAGTGGG?AGAGAGGATT?ACAGGGGTAT?GATACAAACT?TAACAATACT
AF322109 CAAGAGTGGG?AAAGGGAGAT?AAGTGCGCAT?GAAGGAAACA?TCACTATATT
8401 8450
NC_001463 GTTAAAGGAA?TCAGCAGCAA?TGACACAACT?AGCAGAAGAG?CAAGCAAGGA
AF322109 ACTCAAAGAA?TCAGCAAGGA?TAACACAATT?AGCACAACAA?AAGGTACAAA
8451 8500
NC_001463 GGATACCAGA?AGTATGGGAA?AGTTTAAAAG?ACGTCTTTGA?TTGGTCAGGA
AF322109 GAATACCAGA?TGTGTGGACA?GCACTAAGGG?AGTCACTAGG?ATGGACACAA
8501 8550
NC_001463 TGGTTCTCAT?GGCTAAAGTA?TATTCCTATT?ATAGTAGTAG?GATTATTAGG
AF322109 TGGCTGGCTT?GGATAAAATA?CCTTCCCATA?ATAGTAGTAG?GGATATTAGG
8551 8600
NC_001463 ATGCATTCTG?ATAAGAGCTG?TGATATGTGT?ATGTCAACCT?CTTGTGCAGA
AF322109 ATGCATAATC?ATAAGAATAA?TGTTGTGTGT?AGTACAACCA?GTTCTTCAGA
8601 8650
NC_001463 TATACAGAAC?TCTAAGTACC?CCGACATACC?AACGGGTCAC?AGTCATCATG
AF322109 TTTACAGAAC?CTTGACTCAG?ACCAGGTATC?AACAAGTCAA?CTTGGTGATG
8651 8700
NC_001463 GAAACAAGAG?CAGACGTCGC?AGGAGAAAAT?CAGGATTTTG?GC...GATGG
AF322109 GAGACCCGGG?TGCAACTAGA?AGAAGAAGAA?GAAGAAGACG?GAAGGGATGG
8701 8750
NC_001463 CTTAGAGGAA?TCAGACAA..?.CAGCGAAAC?AAGCGAAAGA?GTGACAGTAC
AF322109 TGGAGATGGC?TCAGAGAGAT?GCAGCGATCC?CGACAACAAA?GG...AATTA
8751 8800
NC_001463 AGAAAGCTTG?GAGCCGTGCC?TGGGAGCTTT?GGCAGAACTC?ACCCTGGAAG
AF322109 TGAACGCCTG?GAGGAGAGCT?TGGGTGACTT?GGAGAAACTC?ACCTTGGCAG
8801 8850
NC_001463 GAGCCATGGA?AAAGGGGCCT?GCTGAGGCTG?CTCGTCCTTC?CGCTGACGAT
AF322109 AACACATGGA?AGAATGTGGT?GGTGGCGCCG?TTGGTGATTC?CGCTGACAAT
8851 8900
NC_001463 GGGAATCTGG?ATAAATGGAT?GGCTTGGAGA?ACACCACAAA?AATAAAAAAA
AF322109 CAGAATTTGG?CTCCTTGGAG?AGAATGGAGA?GAACCCCTAA?AAGAAAAATA
8901 8950
NC_001463 GAAAGGGTG.?ACTGTGAGAC?ATGGGCTAAA?GAGGACTAAT?AACAAGCTAG
AF322109 AAAAGGGTGG?ACTGTGAGGA?CTGTG.....?.AGGCCTAGG?AGCGAGATAG
8951 9000
NC_001463 GCCAAATTCC?TGTAAATCAC?TTGGGGGGTT?ATAAGAAAAG?CAAGTTCACT
AF322109 ...AAACTTA?TAGGCCTCTC?TTCCCGG...?......AAAG?CTAACTCACT
9001 9050
NC_001463 ATGACAAAGC?AAAATGTAAA?GGCCAAATTC?CTGTAAATCA?CTTGGGGGGT
AF322109 GTG.......?.AGAGGAATA?G..CAAGTCA?CAGTGA..CA?CT.....GCT
9051 9100
NC_001463 TATAAGAAAA?GCAAGTTCAC?TATGACAAAG?CAAAATGTAA?CCGCAAG...
AF322109 AATTGTACCC?GCAA...CCC?TGAGATCATG?CAAACCACAA?TCCTGAGATT
9101 9150
NC_001463 .TGCTGACAG?ATGTAACAGC?TGACATATCA?GCTGATGCTT?GCTCATGCTG
AF322109 ATGCTGACAT?GTGTAACAGC?TGATGCCTCA?GCTGATGCTT?GCTCATGCTG
9151 9200
NC_001463 ACACTGTAGC?TCTGAGCTGT?ATATAAGGAG?AAGCTTGCTG?CTTGC.ACTT
AF322109 ACAATGTAAC?TAGGAGCTCT?ATATAAACAG?AGCCCTAGAG?CTTGCTACTT
9201 9250
NC_001463 CAGAGTTCTA?GGAGAGTCCC?..........?.TCCT.AGTC?TCTCCTCTCC
AF322109 CAGAGTGCTC?TGAGGAGCTC?GAAGGAAAGA?GTCCTCAGCC?TCTCCTCTCC
9251 9300
NC_001463 GAGGAGGTAC?CGAGACCTCA?AAATAAAGGA?GTGATTGCCT?TACTGCCGA.
AF322109 GAGGAGCTTC?GG....CTCA?TAATAAAGGA?GTGCTTGCTT?CA..ACAGAA
Table 2
PileUp
MSF：759 Type：N Check：?1376 ..
Name：NC_001463(gag720bp)?(SEQ?ID?N0：3)?Len：759 Check：9060 Weight：0
Name：AF322109(gag720bp)?(SEQ?ID?N0：4) Len：759 Check：2316 Weight：0
//
1 50
NC_001463(gag720bp)?ATGGTGAGTC?TAGATAGAGA?CATGGCGAGG?CAAGTCTCCG?GGGGGAAAAG
AF322109(gag720bp)?..........?..........?.ATGGTGAGG?CAGGCCTCCG?GAAGGGGAAA
51 100
NC_001463(gag720bp)?AGATTATCCT?GAGCTCGAAA?AATGTATCAA?GCATGCATGC?AAGATAAAAG
AF322109(gag720bp)?GGAGTACCCC?GAGCTAAAAG?AATGTCTGAA?AAAGGCATGC?AAAATAAAAG
101 150
NC_001463(gag720bp)?TTCGACTCAG?AGGGGAGCAC?TTGACAGAAG?GAAATTGTTT?ATGGTGCCTT
AF322109(gag720bp)?TAAGGGCTGG?GGGGGAGCGC?CTGACAGAAG?GAAATTGTCT?CTGGTGTATA
151 200
NC_001463(gag720bp)?AAAACATTAG?ATTACATGTT?TGAGGACCAT?AAAGAGGAAC?CTTGGACAAA
AF322109(gag720bp)?AAAACACTAG?AGTGTATGTA?TGAGGATTGT?AGGGAGGAAC?CTTGGACCCC
201 250
NC_001463(gag720bp)?AGTAAAATTT?AGGACAATAT?GGCAGAAGGT?GAAGAATCTA?ACTCCTGAGG
AF322109(gag720bp)?AGAAAAATGT?AAACAATTAT?GGAAAAAGTT?GAAGCAGGTA?GAGCCTGAGG
251 300
NC_001463(gag720bp)?AGAGTAACAA?AAAAGACTTT?ATGTCTTTGC?AGGCCACATT?AGCGGGTCTA
AF322109(gag720bp)?AGAGTAGCAA?AGCAGACTAT?AACTCGTTAA?AAGCAACCTT?GGCGGGGATA
301 350
NC_001463(ga9720bp)?ATGTGTTGCC?AAATGGGGAT?GAGACCTGAG?ACATTGCAAG?ATGCAATGGC
AF322109(9ag720bp)?GTCTGTGTGC?AAATGGGAAT?GCAGCCCGAG?ACACTGCAGG?ATGCGATAGC
351 400
NC_001463(gag720bp)?TACAGTAATC?ATGAAAGATG?GGTTACTGGA?ACAAGAGGAA?AAGAAGGAAG
AF322109(gag720bp)?AACCTTAAAC?ATGAGAGATG?AAGT......?..AAAAGGAA?AGGAA..AAG
401 450
NC_001463(gag720bp)?ACAAAAGAGA?AAAGGAAGAG?AGTGTCTTCC?CAATAGTAGT?GCAAGCAGCA
AF322109(gag720bp)?CCATCAGAAG?AAAAGAAGGG?AATATAT..C?CCATATTAGT?GCAGGCAGGA
451 500
NC_001463(gag720bp)?GGAGGGAGAA?GCTGGAAAGC?AGTAGATTCT?GTAATGTTCC?AGCAACTGCA
AF322109(gag720bp)?GGAGGAAGAG?CATGGAGAGC?GGTAGAGCCT?GCTACCTTTC?AGCAGCTCCA
501 550
NC_001463(gag720bp)?AACAGTAGCA?ATGCAGCATG?GCCTCGTGTC?TGAGGACTTT?GAAAGGCAGT
AF322109(gag720bp)?AACAGTGGCA?ATGCAGCATG?GACTAGTATC?AGAAGAATTT?GAAAGGCAGC
551 600
NC_001463(gag720bp)?TGGCATATTA?TGCTACTACC?TGGACAAGTA?AAGACATACT?AGAAGTATTG
AF322109(gag720bp)?TAGCATACTA?TGCCACCACA?TGGACAAGCA?AGGATATCTT?AGAAGTATTA
601 650
NC_001463(gag720bp)?GCCATGATGC?CTGGAAATAG?AGCTCAAAAG?GAGTTAATTC?AAGGGAAATT
AF322109(gag720bp)?GCCATGATGC?CAGGAAATAG?AGCGCAAAAA?GAACTAATAC?AAGGAAAGTT
651 700
NC_001463(gag720bp)?AAATGAAGAA?GCAGAAAGGT?GGAGAAGGAA?TAATCCACCA?CCTCCAGCAG
AF322109(gag720bp)?AAATGAGGAA?GCAGAGAGAT?GGAGAAGGCA?GAATCCACAA?CCTGCGG...
701 750
NC_001463(gag720bp)?GAGGAGGATT?AACAGTGGAT?..........?..........?..........
AF322109(gag720bp)?...GCGGGTT?AACCGTGGAT?CAGATAATGG?GGGTAGGACA?AACGAATCAG
751
NC_001463(gag720bp)?.........
AF322109(gag720bp)?GCAGCGGCA
PileUp
MSF：1347 Type：N Check：2008 ..
Name：NC_001463(gag)?(SEQ?ID?NO：5)?Len：1347 Check：6959 Weight：0
Name：AF322109(gag)?(SEQ?ID?NO：6) Len：1347 Check：5049 Weight：0
//
1 50
NC_001463(gag) ATGGTGAGTC?TAGATAGAGA?CATGGCGAGG?CAAGTCTCCG?GGGGGAAAAG
AF322109(gag) ..........?..........?.ATGGTGAGG?CAGGCCTCCG?GAAGGGGAAA
51 100
NC_001463(gag) AGATTATCCT?GAGCTCGAAA?AATGTATCAA?GCATGCATGC?AAGATAAAAG
AF322109(gag) GGAGTACCCC?GAGCTAAAAG?AATGTCTGAA?AAAGGCATGC?AAAATAAAAG
101 150
NC_001463(gag) TTCGACTCAG?AGGGGAGCAC?TTGACAGAAG?GAAATTGTTT?ATGGTGCCTT
AF322109(gag) TAAGGGCTGG?GGGGGAGCGC?CTGACAGAAG?GAAATTGTCT?CTGGTGTATA
151 200
NC_001463(gag) AAAACATTAG?ATTACATGTT?TGAGGACCAT?AAAGAGGAAC?CTTGGACAAA
AF322109(gag) AAAACACTAG?AGTGTATGTA?TGAGGATTGT?AGGGAGGAAC?CTTGGACCCC
201 250
NC_001463(gag) AGTAAAATTT?AGGACAATAT?GGCAGAAGGT?GAAGAATCTA?ACTCCTGAGG
AF322109(gag) AGAAAAATGT?AAACAATTAT?GGAAAAAGTT?GAAGCAGGTA?GAGCCTGAGG
251 300
NC_001463(gag) AGAGTAACAA?AAAAGACTTT?ATGTCTTTGC?AGGCCACATT?AGCGGGTCTA
AF322109(gag) AGAGTAGCAA?AGCAGACTAT?AACTCGTTAA?AAGCAACCTT?GGCGGGGATA
301 350
NC_001463(gag) ATGTGTTGCC?AAATGGGGAT?GAGACCTGAG?ACATTGCAAG?ATGCAATGGC
AF322109(gag) GTCTGTGTGC?AAATGGGAAT?GCAGCCCGAG?ACACTGCAGG?ATGCGATAGC
351 400
NC_001463(gag) TACAGTAATC?ATGAAAGATG?GGTTACTGGA?ACAAGAGGAA?AAGAAGGAAG
AF322109(gag) AACCTTAAAC?ATGAGAGATG?........AA?GTAAAAGGAA?AGGAA..AAG
401 450
NC_001463(gag) ACAAAAGAGA?AAAGGAAGAG?AGTGTCTTCC?CAATAGTAGT?GCAAGCAGCA
AF322109(gag) CCATCAGAAG?AAAAGAAGGG?AATATATCCC?..ATATTAGT?GCAGGCAGGA
451 500
NC_001463(gag) GGAGGGAGAA?GCTGGAAAGC?AGTAGATTCT?GTAATGTTCC?AGCAACTGCA
AF322109(gag) GGAGGAAGAG?CATGGAGAGC?GGTAGAGCCT?GCTACCTTTC?AGCAGCTCCA
501 550
NC_001463(gag) AACAGTAGCA?ATGCAGCATG?GCCTCGTGTC?TGAGGACTTT?GAAAGGCAGT
AF322109(gag) AACAGTGGCA?ATGCAGCATG?GACTAGTATC?AGAAGAATTT?GAAAGGCAGC
551 600
NC_001463(gag) TGGCATATTA?TGCTACTACC?TGGACAAGTA?AAGACATACT?AGAAGTATTG
AF322109(gag) TAGCATACTA?TGCCACCACA?TGGACAAGCA?AGGATATCTT?AGAAGTATTA
601 650
NC_001463(gag) GCCATGATGC?CTGGAAATAG?AGCTCAAAAG?GAGTTAATTC?AAGGGAAATT
AF322109(gag) GCCATGATGC?CAGGAAATAG?AGCGCAAAAA?GAACTAATAC?AAGGAAAGTT
651 700
NC_001463(gag) AAATGAAGAA?GCAGAAAGGT?GGAGAAGGAA?TAATCCACCA?CCTCCAGCAG
AF322109(gag) AAATGAGGAA?GCAGAGAGAT?GGAGAAGGCA?GAATCCACAA?CCTGCGG...
701 750
NC_001463(gag) GAGGAGGATT?AACAGTGGAT?CAAATTATGG?GGGTAGGACA?AACAAATCAA
AF322109(gag) ...GCGGGTT?AACCGTGGAT?CAGATAATGG?GGGTAGGACA?AACGAATCAG
751 800
NC_001463(gag) GCAGCAGCAC?AAGCTAACAT?GGATCAGGCA?AGGCAAATAT?GCCTGCAATG
AF322109(gag) GCAGCGGCAC?AGGCTAATAT?GGATCAAGCA?AGACAAATAT?GCCTACAATG
801 850
NC_001463(gag) GGTAATAAAT?GCATTAAGAG?CAGTAAGACA?TATGGCGCAC?AGGCCAGGGA
AF322109(gag) GGTTATAACA?GCAATAAGAG?GAGTTAGGCA?TATGGCCCAT?AGACCAGGAA
851 900
NC_001463(gag) ATCCAATGCT?AGTAAAGCAA?AAAACGAATG?AGCCATATGA?AGATTTTGCA
AF322109(gag) ATCCCATGCT?GGTAAGACAA?AAACCAAATG?AGAACTATGA?AGAGTTTGCC
901 950
NC_001463(gag) GCAAGACTGC?TAGAAGCAAT?AGATGCAGAG?CCAGTTACAC?AGCCTATAAA
AF322109(gag) GCAAGGTTGT?TAGAAGCAGT?GGATGCAGAA?CCCGTTACCC?AACCTATAAA
951 1000
NC_001463(gag) AGATTATCTA?AAGCTAACAC?TATCTTATAC?AAATGCATCA?GCAGATTGTC
AF322109(gag) AGAATATTTA?AAGGTAACTC?TGTCTTACAC?AAATGCAAAT?TCGGAATGTC
1001 1050
NC_001463(gag) AGAAGCAAAT?GGATAGAACA?CTAGGACAAA?GAGTACAACA?AGCTAGTGTA
AF322109(gag) AAAAACATAT?GGACAGAGTG?TTGGGGCAAA?GAGTACAGCA?GGCCTCAATA
1051 1100
NC_001463(gag) GAAGAAAAAA?TGCAAGCATG?TAGAGATGTG?GGATCAGAAG?GGTTCAAAAT
AF322109(gag) GAAGAAAAAA?TGCAGGCATG?CAGGGACATC?GGGGGAACAG?CATATCAGAT
1101 1150
NC_001463(gag) GCAATTGTTA?GCACAAGCAT?TAAGGCCAGG?AAAAGGAAAA?GGGAATGGAC
AF322109(gag) GCAGTTGCTT?GCACAAGCCC?TCCGTGGCGG?AAAAGAAGAT?GGGAAAAAAT
1151 1200
NC_001463(gag) AGCCACAAAG?GTGTTACAAC?TGTGGAAAAC?CGGGACATCA?AGCAAGGCAA
AF322109(gag) CTGTAGGGAA?GTGTTATAAC?TGTGGAAGGC?CCGGACACAG?AGCAAAAGAA
1201 1250
NC_001463(gag) TGTAGACAAG?GAATCATATG?TCACAACTGT?GGAAAGAGAG?GACATATGCA
AF322109(gag) TGCAGACAAG?GCATTATATG?TCACAACTGT?GGAAAAAGAG?GGCATATACA
1251 1300
NC_001463(gag) AAAAGAATGC?AGAGGAAAGA?GAGACATAAG?GGGAAAACAG?CAGGGAAACG
AF322109(gag) GAAAAACTGC?A.....AACA?GAAA....AG?AAGAAAGGAG?CAGGGAAACA
1301 1347
NC_001463(gag) GGAGGAGGGG?GATACGTGTG?GTGCCGTCCG?CTCCTCCTAT?GGAATAA
AF322109(gag) TGAGGAGGGG?GCTACGTGTG?GTGCCGTCCG?CACCCCCTAT?GGAGTAA
Table 3
PileUp
MSF：605 Type：N Check：9138 ..
Name：NC_001463(5′)(SEQ?ID?NO：7)Len：605 Check：5398 Weight：0
Name：AF322109(5′)(SEQ?ID?NO：8)Len：605 Check：3740 Weight：0
//
1 50
NC_001463(5′)?..........?....GAGTTC?TAGG...AGA?GTCCCTCCTA?GTCTCTCCTC
AF322109(5′)?GTGAGTGCTC?TGAGGAGCTC?GAAGGAAAGA?GTCC.TC..A?GCCTCTCCTC
51 100
NC_001463(5′)?TCCGAGGAGG?TACCGAGACC?TCAAA?TAAA?GGAGTGATTG?CCTTACTGCC
AF322109(5′)?TCCGAGGAGC?TTCGG....C?TCATAATAAA?GGAGTGCTTG?CTTCA..ACA
101 150
NC_001463(5′)?GAGTGGAGAG?TGATTACTGA?GCGGCCGGTG?TATCGGGAGT?CGTCCCTTAA
AF322109(5′)?GAACTGAG..?......CTGG?TCGTGGTTAT?TATCGGG...?.GACCGAAGT
151 200
NC_001463(5′)?TCTGTGCAAT?ACCAGAGCGG?CTCTCGCAGC?TGGCGCCCAA?CGTGGGGCCC
AF322109(5′)?CCCGTGCAAC?ACCGGGGCGG?TTCTCGCAGC?TGGCGCCCAA?CGTGGGGCTC
201 250
NC_001463(5′)?GAGGAG....?..........?..........?..........?..........
AF322109(5′)?GAGTAGCTTG?AGAAGCTCGA?CTGAGATCTG?AATCCAAGAG?CGACATCAGA
251 300
NC_001463(5′)?....AAGAAA?AGAAAGC...?GGCCCTGAGA?ACTCGGCTTC?TG..AAAAAG
AF322109(5′)?CAGCAAGAAA?TGAGAGTAAT?GAGACCGCGA?GCTCTGCTGC?TGTAAAAAAG
301 350
NC_001463(5′)?AGGAAGAGGA?CAAGTTGCTA?TAGCAACAAG?AGAGAAGAAG?TAGAGCAAAG
AF322109(5′)?AGGAAGTAG.?CGGGTTGCCG?AGGCAACTGC?TCAGAAGAAC?CAGGGGAAAG
351 400
NC_001463(5′)?GTCCAGTGGC?T.CGGAAAAA?GAGGAACTGA?AACTTCGGGG?ACGCCTGAAG
AF322109(5′)?GGCTTCCAGC?AACCTCAAAA?GAGGAACCGA?GACTTCGGGG?ACGCCTGAA.
401 450
NC_001463(5′)?GAGTAAGGTA?AGTGACTCTG?CTGTACGCGG?GGCGAGGCAG?AGGTT.TCCT
AF322109(5′)?..GTAAGGTA?AGTGACTCTG?CTGTACGCGG?GGCGAGGCAT?AGGAGATCCT
451 500
NC_001463(5′)?TCTAAATT.G?AAAGAGAAGT?GTTGCTGCGA?GAGGTCTTGG?TGGTCGAGAA
AF322109(5′)?TCTATTCTAG?GAAGAGAAGC?GCTGTTCTGG?GAGGTCTTGG?CGACCGAGAA
501 550
NC_001463(5′)?TCCTGTACAA?AAAAAAGGAG?GGATCTCGGT?CAGGACCAGG?ACCCCTGGGA
AF322109(5′)?TCTTGTT...?AAATAAGCCA?GGATCTCGAT?CAGGACCAAG?ACCCCTCAGG
551 600
NC_001463(5′)?GTAATACAAC?AGCAACACCG?TAAGAAAATC?CGCC......?..........
AF322109(5′)?AGAGGGTATA?GACAGCGTGG?TAAGAAA.TC?CGCCGTGGTG?AGTCTAGATA
601
NC_001463(5′)?.....
AF322109(5′)?GAGAC
Table 4
PileUp
MSF：3338 Type：N Check：5428 ..
Name：NC_001463(pol) (SEQ?ID?NO：9)Len：3338 Check：8114 Weight：0
Name：AF322109(pol)?(SEQ?ID?NO：10)?Len：3338 Check：7314 Weight：0
//
1 50
NC_001463(pol) ..........?........AT?GTCACAACTG?TGGAAAGAGA?GGACATATGC
AF322109(pol) ATGCAGACAA?GGCATTATAT?GTCACAACTG?TGGAAAAAGA?GGGCATATAC
51 100
NC_001463(pol) AAAAAGAATG?CAGAGGAAAG?AGAGACATAA?GGGGAAAACA?GCAGGGAAAC
AF322109(pol) AGAAAAACTG?CA.....AAC?AGAAA....A?GAAGAAAGGA?GCAGGGAAAC
101 150
NC_001463(pol) GGGAGGAGGG?GGATACGTGT?GGTGCCGTCC?GCTCCTCCTA?TGGAATAACT
AF322109(pol) ATGAGGAGGG?GGCTACGTGT?GGTGCCGTCC?GCACCCCCTA?TGGAGTAACG
151 200
NC_001463(pol) TCAGCACCAC?CTATGGTTCA?GGTCCGCATA?GGTTCCCAGC?AGAGGAACTT
AF322109(pol) CAAGCACCAC?TAATAGTTAG?GGTACAAATA?GGGAATCAGG?AGAAACAATT
201 250
NC_001463(pol) GTTATTTGAT?ACCGGGGCGG?ACCGAACTAT?AGTTAGATGG?CATGAGGGCT
AF322109(pol) ATTATTTGAC?ACAGGGGCAG?ATAAAACGAT?AGTAAGAATG?CATGATGGAA
251 300
NC_001463(pol) CGGGAAACCC?AGCCGGAAGG?ATAAAACTGC?AAGGAATAGG?AGGAATAGTA
AF322109(pol) CAGGGATTCC?AAACGGAAGA?ATAAAATTAC?AAGGGATAGG?AGGAATAGTA
301 350
NC_001463(pol) GAAGGAGAAA?AATGGAATAA?TGTAGAATTA?GAATATAAAG?GAGAAACAAG
AF322109(pol) GAAGGAGAAA?AATGGAATAA?AGTACCCATG?ACATATAAGG?GAGAAACATC
351 400
NC_001463(pol) AAAGGGAACA?ATAGTAGTGT?TACCACAAAG?TCCAGTAGAA?GTATTAGGAC
AF322109(pol) CTGCCCAAGC?TTGGTTGTGC?TAAGAGATAG?CCCAGTAGAA?GTATTGGGAA
401 450
NC_001463(pol) GAGATAACAT?GGCCCGATTT?GGAATAAAGA?TAATAATGGC?AAATTTAGAG
AF322109(pol) GAGATAACAT?GGAAGCATTC?GGCGTAACCC?TAATAATGGC?AAATTTAGAA
451 500
NC_001463(pol) GAAAAAAGAA?TCCCAATTAC?AAAAGTAAAA?TTGAAAGAGG?GATGTACGGG
AF322109(pol) GATAAGAAAA?TTCCCACAAT?ACCAGTAGAA?TTGAAAGAAG?GATGTAAAGG
501 550
NC_001463(pol) TCCACATGTC?CCACAATGGC?CATTAACAGA?AGAGAAATTA?AAAGGTCTAA
AF322109(pol) GCCACATGTG?CCCCAGTGGC?CATTAACAGC?AGAGAAATTA?CAAGGACTAA
551 600
NC_001463(pol) CAGAAATCAT?AGATAAATTA?GTGGAAGAAG?GAAAACTAGG?AAAGGCACCC
AF322109(pol) CAGGAATAGT?AGAAAAATTA?CTACAGGAAG?GAAAATTGGC?AGAGGCCCCA
601 650
NC_001463(pol) CCACATTGGA?CATGTAATAC?TCCAATCTTT?TGCATAAAAA?AGAAATCAGG
AF322109(pol) GAGGGATGGA?CGTGGAACAC?GCCCATCTTC?TGCATAAAAA?AGAAGTCAGG
651 700
NC_001463(pol) GAAGTGGAGA?ATGTTAATAG?ATTTCAGAGA?ATTGAACAAA?CAGACAGAAG
AF322109(pol) AAAATGGAGA?ATGTTAATAG?ATTTTAGGGA?ATTAAATAAG?CAAACAGCAG
701 750
NC_001463(pol) ATTTAACAGA?AGCGCAGTTA?GGACTCCCGC?ATCCGGGAGG?ACTACAAAAG
AF322109(pol) ATTTAGCAGA?AGCGCAGCTA?GGACTGCCAC?ACCCAGGAGG?GTTGCAAAGG
751 800
NC_001463(pol) AAAAAACATG?TTACAATATT?GGACATAGGA?GATGCATATT?TTACTATACC
AF322109(pol) AAAAAGAATG?TAACAATTCT?GGACATAGGA?GATGCATATT?TCACAATTCC
801 850
NC_001463(pol) CCTATATGAA?CCATATCGAG?AGTACACATG?TTTTACTCTA?TTAAGTCCTA
AF322109(pol) CTTATACGAG?CCCTATCAGA?AATATACATG?CTTCACACTC?CTAAGTCCTA
851 900
NC_001463(pol) ATAATCTAGG?ACCATGTAAA?AGATACTATT?GGAAAGTGCT?GCCACAAGGT
AF322109(pol) ACAATTTGGG?ACCATGTAAA?AGGTATTATT?GGAAAGTATT?ACCCCAGGGA
901 950
NC_001463(pol) TGGAAATTGA?GTCCATCTGT?ATATCAATTT?ACTATGCAGG?AGATCTTAGA
AF322109(pol) TGGAAATTGA?GCCCAGCTGT?ATATCAATTC?ACCATGCAAA?GGTTGTTAAA
951 1000
NC_001463(pol) GGATTGGATA?CAGCAGCATC?CAGAAATTCA?ATTTGGCATA?TATATGGATG
AF322109(pol) AGGATGGATA?CAACAGCATA?AAAACATACA?ATTTGGAATA?TATATGGATG
1001 1050
NC_001463(pol) ATATTTACAT?AGGAAGTGAT?TTAGAAATTA?AAAAGCATAG?AGAAATAGTG
AF322109(pol) ATATCTATAT?TGGAAGTGAT?CTAACGATAG?CCCAACATAG?GAAGATAATA
1051 1100
NC_001463(pol) AAAGATTTAG?CCAATTATAT?TGCCCAATAT?GGATTCACTC?TGCCAGAAGA
AF322109(pol) GAAGAATTAG?CCTCATTTAT?AGAACAATTT?GGGTTTACAT?TACCAGAAGA
1101 1150
NC_001463(pol) GAAGAGACAA?AAGGGATATC?CAGCAAAATG?GCTAGGATTT?GAACTACACC
AF322109(pol) TAAGAGACAA?GAGGGCTATC?CAGCAAAATG?GCTAGGATTC?GAGCTACATC
1151 1200
NC_001463(pol) CGCAGACCTG?GAAATTTCAG?AAGCATACAT?TACCTGAATT?AACAAAGGGA
AF322109(pol) CAGAAAAATG?GAAATATCAA?AAGCATAAAT?TGCCGGAATT?ACAAGAGGGG
1201 1250
NC_001463(pol) ACAATAACAT?TAAATAAATT?ACAGAAATTA?GTAGGAGAAT?TAGTATGGAG
AF322109(pol) GTAATAACCC?TGAACAAATT?ACAGAAGATA?GTAGGGGAAT?TAGTGTGGAG
1251 1300
NC_001463(pol) ACAATCCATA?ATTGGGAAAA?GCATTCCTAA?CATTCTGAAA?TTAATGGAAG
AF322109(pol) ACAATCCTTG?ATAGGAAAGA?GCATCCCCAA?TATCATAAAA?TTAATGGAAG
1301 1350
NC_001463(pol) GAGATAGAGA?ATTACAAAGT?GAAAGAAAAA?TTGAAGAAGT?ACATGTGAAA
AF322109(pol) GAGATCGCGC?ATTACAAAGT?GAAAGGAAAA?TAGAAAGAAT?ACATGTACAA
1351 1400
NC_001463(pol) GAATGGGAAG?CATGTAGGAA?AAAATTAGAA?GAAATGGAAG?GAAATTATTA
AF322109(pol) GAATGGGAAG?CATGTCAAAA?GAAATTAGAT?GAAATGGTAG?GAAATTATTA
1401 1450
NC_001463(pol) TAATAAAGAC?AAAGATGTCT?ATGGACAATT?GGCTTGGGGA?GACAAAGCTA
AF322109(pol) CAGAGAAGAA?GAAGATATCT?ATGGACAAAT?AACTTGGGGG?GATAAGGCAA
1451 1500
NC_001463(pol) TAGAATATAT?AGTGTATCAG?GAGAAAGGGA?AACCATTATG?GGTAAATGTG
AF322109(pol) TAAAATACAT?AGTATTCCAA?AGGAAAGGGG?AACCCCTATG?GGTAAATGTA
1501 1550
NC_001463(pol) GTTCACAATA?TAAAGAACCT?AAGCATCCCG?CAACAGGTTA?TTAAAGCAGC
AF322109(pol) GTACATGACA?TAAAAAATTT?GAGTCTCCCA?CAGCAAGTGA?TAAAAGCAGC
1551 1600
NC_001463(pol) GCAAAAATTA?ACCCAAGAAG?TCATCATTAG?GACAGGAAAA?ATACCATGGA
AF322109(pol) ACAGAAATTA?ACCCAGGAAG?TAATCATAAG?AACAGGAAAA?ATCCCATGGC
1601 1650
NC_001463(pol) TATTGTTGCC?AGGGAAAGAA?GAAGATTGGA?GACTAGAATT?GCAATTAGGG
AF322109(pol) TGCTACTACC?AGGAAGAGAA?GAAGACTGGA?GATTAGAACT?GCAGGTAGGG
1651 1700
NC_001463(pol) AACATCACAT?GGATGCCAAA?ATTTTGGTCC?TGTTATCGAG?GA.CATACAA
AF322109(pol) AACATCACGT?GGATGCCATC?ATTTTGGTCA?TGTTATCGAG?GAGCACCCAA
1701 1750
NC_001463(pol) GATGGAGAAA?AAGAAATATA?ATAGAAGAAG?TAGTAGAAGG?GCCTACATAT
AF322109(pol) G.TGGAAAAG?AAGGAACATA?GTGGCAGCAG?TGGTAGATGG?ACCGACATAT
1751 1800
NC_001463(pol) TATACAGATG?GAGGAAAAAA?GAATAAAGTA?GGAAGTCTAG?GGTTCATAGT
AF322109(pol) TATACAGATG?GGGGAAAGAA?AAACGCACAG?GGAAGCTTTG?GCTTCATCTC
1801 1850
NC_001463(pol) ATCAACAGGG?GAAAAATTTA?GAAAGCATGA?AGAGGGCACA?AACCAGCAAC
AF322109(pol) CCCAACAGGA?GAAAAGTTCA?GAAGGCATGA?AGATGGAACT?AATCAGGTAT
1851 1900
NC_001463(pol) TAGAATTAAG?AGCCATAGAG?GAAGCTCTAA?AACAAGGGCC?TCAAACAATG
AF322109(pol) TAGAATTAAG?GGCAATAGAA?GATCCATGTA?AACAAGGACC?TGAAAGCATG
1901 1950
NC_001463(pol) AATTTAGTAA?CAGATAGTAG?ATATGCATTT?GAATTTTTAT?TAAGAAATTG
AF322109(pol) AACATTGTAA?CTGACAGCAG?GTATGCTTAT?GAATTCATGC?TCCGAAACTG
1951 2000
NC_001463(pol) GGATGAAGAA?GTAATAAAGA?ATCCAATTCA?AGCAAGAATT?ATGGAAATTG
AF322109(pol) GGATGAACAG?GTCATAAGAA?ACCCCATTCA?GGCAAGAATC?ATGGCAGAAG
2001 2050
NC_001463(pol) CCCACAAGAA?AGATAGGATA?GGAGTGCATT?GGGTGCCAGG?ACATAAAGGG
AF322109(pol) TGCACAAGAA?AAAGCAGGTA?GGAATACACT?GGGTGCCAGG?GCATAAAGGA
2051 2100
NC_001463(pol) ATTCCCCAAA?ATGAAGAAAT?AGACAAATAT?ATTTCGGAAA?TATTTCTTGC
AF322109(pol) ATACCTCAGA?ATGAAGAGAT?AGACCAGTAC?ATATCAGAAG?TATTCTTAGC
2101 2150
NC_001463(pol) AAAAGAAGGA?GAAGGAATTC?TCCCAAAAAG?AGAAGAGGAT?GCAGGGTATG
AF322109(pol) AGGAGAAGGA?ACAGGGATAT?GTGAAAAAAG?GAAGGAAGAT?GCTGGATATG
2151 2200
NC_001463(pol) ATTTAATATG?CCCAGAAGAG?GTTACCATAG?AGCCAGGACA?AGTGAAATGC
AF322109(pol) ATTTATTATG?CCCGCATGAG?GTAATACTTA?AACCCCAAGA?AGTAAAACGG
2201 2250
NC_001463(pol) ATCCCCATAG?AGCTAAGATT?AAATTTAAAG?AAATCACAAT?GGGCTATGAT
AF322109(pol) ATCCCAATAG?ACCTAAAATT?AAAATTGAAA?GAAAAGCAAT?GGGCCATGAT
2251 2300
NC_001463(pol) TGCTACAAAA?AGCAGCATGG?CTGCCAAAGG?AGTGTTCACA?CAAGGAGGAA
AF322109(pol) AAGTGGGAAA?AGTAGCGTTG?CAGCAAAAGG?AATATTTGTA?CAAGGAGGCA
2301 2350
NC_001463(pol) TCATAGACTC?AGGATATCAG?GGACAAATAC?AGGTAATAAT?GTATAATAGC
AF322109(pol) TAATAGATTC?AGGGTATCAG?GGACAAGTAC?AAGTCATCCT?ATATAATAGT
2351 2400
NC_001463(pol) AATAAAATAG?CAGTAGTCAT?ACCCCAAGGG?AGAAAATTTG?CACAATTAAT
AF322109(pol) AATAAGATAG?AGGTCAAAAT?ACCACAAGGC?AGGAAATTTG?CCCAATTAAT
2401 2450
NC_001463(pol) ATTAATGGAT?AAAAAGCATG?GAAAATTGGA?ACCCTGGGGG?GAAAGCAGAA
AF322109(pol) ATTAATGAAC?TTACAACATG?AAGAATTAGA?AGAATGGGGA?AAGGAAAGAA
2451 2500
NC_001463(pol) AAACAGAAAG?GGGAGAAAAA?GGATTTGGGT?CTACAGGAAT?GTATTGGATA
AF322109(pol) AAACAGAAAG?AGGAACAAAA?GGATTTGGGT?CTACAGGAGC?ATTTTGGATA
2501 2550
NC_001463(pol) GAAAATATTC?CTCTGGCAGA?GGAAGACCAC?ACAAAATGGC?ATCAAGATGC
AF322109(pol) GAGAATATTC?CCCAAGCAGA?GGAAGAACAT?TACAAATGGC?ATCAAGATGC
2551 2600
NC_001463(pol) CCGATCATTG?CATCTAGAAT?TTGAAATTCC?AAGAACAGCA?GCAGAAGACA
AF322109(pol) TAGATCTCTG?CAGCTAGAAT?TCAAGATACC?TAGAGCAGCA?GCAGAAGACA
2601 2650
NC_001463(pol) TAGTAAATCA?ATGTGAAATA?TGCAAAGAAG?CGAGGACACC?TGCAGTAATT
AF322109(pol) TTATACAGCA?CTGTGAGGTA?TGTCAAGAAG?GCAAACCCGC?AGCGATCACG
2651 2700
NC_001463(pol) AGAGGCGGAA?ACAAAAGGGG?GGTAAATCAT?TGGCAAGTGG?ATTATACCCA
AF322109(pol) AGAGGGGGAA?ATAAAAGAGG?AATAGATCAT?TGGCAGGTAG?ACTATACACA
2701 2750
NC_001463(pol) TTATGAAAAT?ATCATACTAT?TAGTATGGGT?AGAAACAAAT?TCAGGACTAA
AF322109(pol) TTACAAAGAA?CACATAATAT?TAGTATGGGT?AGAGACTAAT?TCAGGATTAA
2751 2800
NC_001463(pol) TATATGCAGA?AAAAGTAAAA?GGAGAATCAG?GGCAAGAATT?CAGAATAAAA
AF322109(pol) TATTTGCAGA?GAAAGTAAAA?GGAGAATCAG?GACAAGAATT?TAGGATGCAG
2801 2850
NC_001463(pol) GTGATGCATT?GGTATGCATT?ATTTGGTCCA?GAGTCATTGC?AGTCAGACAA
AF322109(pol) ACATTGAAAT?GGTATGCTTT?GTTTCAACCA?AAATCAGTGC?AATCAGATAA
2851 2900
NC_001463(pol) TGGACCTGCA?TTTGCAGCAG?AGCCCACACA?GCTGTTAATG?CAATACCTAG
AF322109(pol) TGGGACAGCC?TTCACAGCTG?AGGCTACGCA?GCATCTAATG?AAGTATTTAG
2901 2950
NC_001463(pol) GAGTAAAACA?CACAACAGGC?ATACCTTGGA?ATCCACAGTC?TCAGGCTATA
AF322109(pol) GGATTCAGCA?CACTACGGGT?ATTCCGTGGA?ACCCCCAGTC?ACAAAGTTTA
2951 3000
NC_001463(pol) GTAGAAAGGG?CACATCAACT?ATTGAAAAGC?ACTTTAAAGA?AGTTCCAGCC
AF322109(pol) GTAGAAAGAG?CTCATCAAAC?ATTAAAACAC?ATGTTAGAAA?AATTAGAACC
3001 3050
NC_001463(pol)?ACAATTTGTC?GCTGTAGAAT?CAGCCATAGC?AGCAGCCCTA?GTCGCCATAA
AF322109(pol)?ACAATTTGTG?GCCCTACAGT?CTGCCATCGC?AGCCACTCTA?GTTGCGCTCA
3051 3100
NC_001463(pol)?ATATAAAAAG?AAAGGGTGGG?CTGGGGACAA?GCCCTATGGA?TATTTTTATA
AF322109(pol)?ATATAAAAAG?AAAGGGTGGA?CTAGGGGCAA?GCCCTATGGA?TATTTACATA
3101 3150
NC_001463(pol)?TATAATAAAG?AACAGAAAAG?AATAAATAAT?AAATATAATA?AAAATTCTCA
AF322109(pol)?TATAATAAGG?AGCAACAAAG?ACAACAAGAT?AATAGTAATA?AATTAATTCA
3151 3200
NC_001463(pol)?AAAAATTCAA?TTCTGTTATT?ACAGAATAAG?GAAAAGAGGA?CATC.AGGAG
AF322109(pol)?GAAAA...AA?TTTTGTTATT?ACAGGATCAG?AAAAAGAGGC?CATCCAGGAG
3201 3250
NC_001463(pol)?AGTGGAAAGG?ACCAACCCAG?GTACTGTGGA?AAGGGGAAGG?AGCCAATTGT
AF322109(pol)?AGTGGAACGG?CCCAACTGAG?GTACTGTGGG?AAGGGGAAGG?AGCCA.TAGT
3251 3300
NC_001463(pol)?GGTAAAGGAT?ATAGAAAGTG?AAAAGTATTT?AGTAATACCT?TACAAAGATG
AF322109(pol)?AGTTAAAGAC?AAAGAAAGTG?ATAGATATCT?AGTCATCCCA?TATAAAGATG
3301 3338
NC_001463(pol)?CAAAATTCAT?CCCGCCACCA?ACAAAAGAAA?AGGAATAA
AF322109(pol)?CAAAATTTAT?TCCGCCACCG?TCGGAACAGA?AGGGATAG
Table 5
PileUp
MSF：408 Type：N Check：517 ..
Name：NC_001463(rev)?(SEQ?ID?NO：11)?Len：408 Check：7287 Weight：0
Name：AF322109(rev)?(SEQ?ID?NO：12) Len：408 Check：3230 Weight：0
//
1 50
NC_001463(rev) ATGGATGCTG?GGGCCAGATA?CATGCGCTTA?ACTGGGAAGG?AAAACTGGGT
AF322109(rev) ..........?..........?..........?..........?..........
51 100
NC_001463(rev) TGAAGTAACC?ATGGACGGAG?AGAAGGAAAG?GAAAAGAGAA?GGTTTCACTG
AF322109(rev) ..........?ATGG.CGGAG?ATAAGAAAAG?..A.AGCAAA?GGAGCCACTA
101 150
NC_001463(rev) CGGGACAGCA?AGATATACAG?AACTCTAAGT?ACCCCGACAT?ACCAACGGGT
AF322109(rev) ATCCAGGACC?AGGTATCAAC?AAGTCAACTT?GGTGATGGAG?ACC..CGGGT
151 200
NC_001463(rev) CACAGTCATC?ATGGAAACAA?GAGCAGACGT?CGCAGGAGAA?AATCAGGATT
AF322109(rev) ..........?..GCAACTAG?AAGAAGAAGA?AGAAGAAGAC?GGAAGGGATG
201 250
NC_001463(rev) TTGGCGATGG?CTTAGAGGAA?TCAGACAACA?GCGAAACAAG?CGAAAGAGTG
AF322109(rev) GTGGAGATGG?CTCAGAGAGA?TG......CA?GCGATCCCGA?CAACAAAGGA
251 300
NC_001463(rev) ACAGTACAGA?AAGCTTGGAG?CCGTGCCTGG?GAGCTTTGGC?AGAACTCACC
AF322109(rev) A...TTATGA?ACGCCTGGAG?GAGAGCTTGG?GTGACTTGGA?GAAACTCACC
301 350
NC_001463(rev) CTGGAAGGAG?CCATGGAAAA?GGGGCCTGCT?GAGGCTGCTC?GTCCTTCCGC
AF322109(rev) TTGGCAGAAC?ACATGGAAGA?ATGTGGTGGT?GGCGCCGTTG?GTGATTCCGC
351 400
NC_001463(rev) TGACGATGGG?AATCTGGATA?AATGGATGGC?TTGGAGAACA?CCACAAAAAT
AF322109(rev) TGACAATCAG?AATTTGGCTC?CTTGGAGAGA?ATGGAGAGAA?CCCCTAAAAG
401
NC_001463(rev) AA......
AF322109(rev) AAAAATAA
Table 6
PileUp
MSF：691 Type：N Check：6528 ..
Name：NC_001463(vif)?(SEQ?ID?NO：13) Len：691 Check：5882 Weight：0
Name：AF322109(vif)?(SEQ?ID?NO：14) Len：691 Check：646 Weight：0
//
1 50
NC_001463(vif) ATGCAAAATT?CATCCCGCCA?CCAACAAAAG?AAAAGGAATA?AAAAACCTGG
AF322109(vif) ATGCAAAATT?TATTCCGCCA?CCGTCGGAAC?AGAAGGGATA?GAAGAATAGG
51 100
NC_001463(vif) ACCAGAATTA?CCCTTAGCAC?TATGGATACA?TATAGCAGAA?AGCATTAATG
AF322109(vif) TCCAGAATTG?CCTTTATCTT?TATGGACTTA?TACAGCATAC?AGCATAAATA
101 150
NC_001463(vif) GGGATAGCTC?ATGGTACATA?ACAATGAGAC?TGCAACAGAT?GATGTGGGGA
AF322109(vif) AAGATCCCGC?ATGGTATACA?ACCCTAAGAC?TGCAGCAAAT?GATGTGGCAT
151 200
NC_001463(vif) AAAAGAGGAA?ATAAGTTACA?ATATAAGAAT?GAAGACAGGG?AATATGAAAA
AF322109(vif) AGGAGGGGAA?ATAAATTGAC?ATATGTCAGG?GAAAATGCAC?AGTACGAGGA
201 250
NC_001463(vif) TTGGGAAATT?ACATCATGGG?GATGGAAAAT?GCACCTAAGG?AGAGTGAAAC
AF322109(vif) GTGGGAAATG?ACCTCGTATG?AGTGGAGGAT?AAGAATGAGA?AGGGACAAAA
251 300
NC_001463(vif) AATGGATACA?AGACAACAGG?AGAGGAAGC.?CCATGGCAGT?ACAAAGTAGG
AF322109(vif) CAAAAAGTCA?TC.CAAGAGG?GCATACTTCG?CCATGGCAAT?ATCGGAGACA
301 350
NC_001463(vif) AGGAACATGG?AAAAGTATAG?GAGTGTGGTT?CCTGCAAGCA?GGAGATTACA
AF322109(vif) GGATGGATGG?AAGGATGTGG?GAACGTGGTT?CCTACAGCCA?GGGGACTATA
351 400
NC_001463(vif) GAAAGGTAGA?CAGGCACTTC?TGGTGGGCAT?GGAGGATACT?GATATGTTCC
AF322109(vif) GAAAGGCGGA?TCAGCAGTTC?TGGTTCGCTT?GGAGAATAGT?GTCGTGTTCA
401 450
NC_001463(vif) TGCAGGAAAG?AAAAGTTTGA?TATAAGAGAA?TTTATGAGAG?GAAGACATAG
AF322109(vif) TGTAAAAAGG?AAGGATTTAA?CATAAGAGAA?TTTATGCTAG?GTACCCATAG
451 500
NC_001463(vif) ATGGGATTTG?TGCAAATCCT?GTGCTCAAGG?AGAAGTAGTA?AAGCATACTA
AF322109(vif) ATGGGATTTG?TGTAAGTCGT?GTTGCCAGGG?TGAAGTAGTA?AAGAGAACAC
501 550
NC_001463(vif) GAACAAAAAG?TCTGGAAAGA?CTAGTACTGC?TACAGATGGT?AGAACAGCAT
AF322109(vif) AACCCTACAC?CTTGCAAAGG?CTCACGTGGC?TTAAATTAAC?AGAAGACCAT
551 600
NC_001463(vif) GTGTTTCAAG?TATTGCCATT?GTGGAGAGCC?AGGAGAAGTA?GTACAACAGA
AF322109(vif) GTATTTCAAG?TAATGCCCTT?GTGGAGAGCT?CGCAAAGGGA?TTACCATAGA
601 650
NC_001463(vif) TTTCCCATGG?TGCAGGGACA?CAACGGGATA?CACGCATGCG?TGGTCTGTCC
AF322109(vif) CTTTCCCTGG?TGCAGGGACA?CAAAAGGATT?CCTGGAGCCG?TGGACAACGC
651 691
NC_001463(vif) AGGAGTGCTG?GTTGATGGAA?TATCTCTTAG?AGGATGAGTG?A
AF322109(vif) AAGAGTGTTG?GCAAATAGAG?TATCCCTTGG?AGGATGAGTG?A
Table 7
PileUp
MSF：736 Type：N Check：513 ..
Name：NC_001463(gag720bp)(SEQ?ID?NO：15) Len：736?Check：4701 Weight：0
Name：>AF015181?(SEQ?ID?NO：16) Len：736 Check：5812 Weight：0
//
1 50
NC_001463(gag720bp) .ATGGTGAGT?CTAGATAGAG?ACATGGCGAG?GCAAGTCTCC?GGGGGGAAAA
>AF015181 GCTGTAGACT?CTGTAATGTT?CCAACAA.AT?GCAAA....C?AGTAGCAATG
51 100
NC_001463(gag720bp) GAGATTATCC?TGAGCTCGAA?AAATGTATCA?AGCATGCATG?CAAGATAAAA
>AF015181 CAGCATGGCC?TCGTGTCCGA?GGATTTTGAA?AGACAGTTAG?CAT.ATTATG
101 150
NC_001463(gag720bp) GTTCGACTCA?GAGGGG..AG?CACTTGACAG?AAGGAAATTG?TTTATGGTGC
>AF015181 CTACTACCTG?GACAAGTAAA?GACATACTAG?AAGTA..TTG?GCCATGATGC
151 200
NC_001463(gag720bp) CTTAAAACA.?...TTAGATT?..ACATGTTT?GAGGACCAT.?.AAAGAGGAA
>AF015181 CTGGGAATAG?GGCTCAGAAA?GAACTTATTC?AAGGGAAATT?GAATGAAGAA
201 250
NC_001463(gag720bp) CCTTGGACAA?AAGTAAAATT?TAGGACAATA?TGGCAGAAGG?.TGAAGAATC
>AF015181 GCA..GACAG?GTGGAGAAG.?..GAACAATC?CACCAGGAGG?ATTAACAGTG
251 300
NC_001463(gag720bp) TAACTCCTGA?GGAGAGTAAC?AAAAAAGACT?TTATGTCTTT?GCAGGCCACA
>AF015181 GATCAAATTA?TGGGGGTAGG?ACAAACAAAT?CAAGCA....?GCAGCACAAG
301 350
NC_001463(gag720bp) TTAGCGGGTC?TAATGTGTTG?CCAAATGGGG?ATGAGACCTG?AGACATTGCA
>AF015181 CTAACATGGA?TCAGGCAA.G?ACAAATATGC?CT...ACAAT?GGGTAATAAA
351 400
NC_001463(gag720bp) AGATGCAATG?GCTACAGTAA?TCATGAAAGA?TGGGTTACTG?GAACAAGAGG
>AF015181 CGCCTTAAGA?GCAGTAAGGC?ATATGGCTCA?TAGGCCAGGG?AATCCAATGC
401 450
NC_001463(gag720bp) AAAAGAAGGA?AGACAAAAGA?GAAAAGGAAG?AGAGTGTCTT?CCCAATAGTA
>AF015181 TAGTAAAGCA?A...AAAACA?AATGAGCCAT?ATGAAGAATT?TGCAGCAAGA
451 500
NC_001463(gag720bp) GTGCAAGCAG?CA..GGAGGG?AGAAGCTGGA?AAGCAGTAGA?TTCTGTAATG
>AF015181 CTGCTAGAAG?CAATAGATGC?AGAAGCGGTT?ACACAGCCCA?TAAAAGAGTA
501 550
NC_001463(gag720bp) T.TCCAGC.A?ACTGCAAACA?GTAGCAATGC?AGCATGGCCT?CGTGTCTGAG
>AF015181 TCTAAAGCTA?ACATTATCCT?ATACAAATGC?AGC.....CT?CA........
551 600
NC_001463(gag720bp) GACTTTGAAA?GGCAGTTGGC?ATATTATGCT?ACTACCTGGA?CAAGTAAAGA
>AF015181 GATTGTCAAA?AGCAAATGG.?AGAGAGTGCT?AGGACAAAGA?...GTACA.A
601 650
NC_001463(gag720bp) CATACTAGAA?GTATTGGCCA?TGATGCCTGG?AAATAGAGCT?CAAAAGGAGT
>AF015181 CAGGCTAGT.?GTAGAAAAAA?AAATGCAAGC?ATGT......?..........
651 700
NC_001463(gag720bp) TAATTCAAGG?GAAATTAAAT?GAAGAAGCAG?AAAGGTGGAG?AAGGAATAAT
>AF015181 ..........?..........?..........?..........?..........
701 736
NC_001463(gag720bp) CCACCACCTC?CAGCAGGAGG?AGGATTAACA?GTGGAT
>AF015181 ..........?..........?..........?......
PileUp
MSF：1347 Type：N Check：939 ..
Name：NC_001463(gag)?(SEQ?ID?NO：17)?Len：1347 Check：6959 Weight：0
Name：>AF015181?(SEQ?ID?NO：18) Len：1347 Check：3980 Weight：0
//
1 50
NC_001463(gag) ATGGTGAGTC?TAGATAGAGA?CATGGCGAGG?CAAGTCTCCG?GGGGGAAAAG
>AF015181 ..........?..........?..........?..........?..........
51 100
NC_001463(gag) AGATTATCCT?GAGCTCGAAA?AATGTATCAA?GCATGCATGC?AAGATAAAAG
>AF015181 ..........?..........?..........?..........?..........
101 150
NC_001463(gag) TTCGACTCAG?AGGGGAGCAC?TTGACAGAAG?GAAATTGTTT?ATGGTGCCTT
>AF015181 ..........?..........?..........?..........?..........
151 200
NC_001463(gag) AAAACATTAG?ATTACATGTT?TGAGGACCAT?AAAGAGGAAC?CTTGGACAAA
>AF015181 ..........?..........?..........?..........?..........
201 250
NC_001463(gag) AGTAAAATTT?AGGACAATAT?GGCAGAAGGT?GAAGAATCTA?ACTCCTGAGG
>AF015181 ..........?..........?..........?..........?..........
251 300
NC_001463(gag) AGAGTAACAA?AAAAGACTTT?ATGTCTTTGC?AGGCCACATT?AGCGGGTCTA
>AF015181 ..........?..........?..........?..........?..........
301 350
NC_001463(gag) ATGTGTTGCC?AAATGGGGAT?GAGACCTGAG?ACATTGCAAG?ATGCAATGGC
>AF015181 ..........?..........?..........?..........?..........
351 400
NC_001463(gag) TACAGTAATC?ATGAAAGATG?GGTTACTGGA?ACAAGAGGAA?AAGAAGGAAG
>AF015181 ..........?..........?..........?..........?..........
401 450
NC_001463(gag) ACAAAAGAGA?AAAGGAAGAG?AGTGTCTTCC?CAATAGTAGT?GCAAGCAGCA
>AF015181 ..........?..........?..........?..........?..........
451 500
NC_001463(gag) GGAGGGAGAA?GCTGGAAAGC?AGTAGATTCT?GTAATGTTCC?AGCAACTGCA
>AF015181 ..........?........GC?TGTAGACTCT?GTAATGTTCC?AACAAATGCA
501 550
NC_001463(gag) AACAGTAGCA?ATGCAGCATG?GCCTCGTGTC?TGAGGACTTT?GAAAGGCAGT
>AF015181 AACAGTAGCA?ATGCAGCATG?GCCTCGTGTC?CGAGGATTTT?GAAAGACAGT
551 600
NC_001463(gag) TGGCATATTA?TGCTACTACC?TGGACAAGTA?AAGACATACT?AGAAGTATTG
>AF015181 TAGCATATTA?TGCTACTACC?TGGACAAGTA?AAGACATACT?AGAAGTATTG
601 650
NC_001463(gag) GCCATGATGC?CTGGAAATAG?AGCTCAAAAG?GAGTTAATTC?AAGGGAAATT
>AF015181 GCCATGATGC?CTGGGAATAG?GGCTCAGAAA?GAACTTATTC?AAGGGAAATT
651 700
NC_001463(gag) AAATGAAGAA?GCAGAAAGGT?GGAGAAGGAA?TAATCCACCA?CCTCCAGCAG
>AF015181 GAATGAAGAA?GCAGACAGGT?GGAGAAGGAA?CAATCCACCA?..........
701 750
NC_001463(gag) GAGGAGGATT?AACAGTGGAT?CAAATTATGG?GGGTAGGACA?AACAAATCAA
>AF015181 ..GGAGGATT?AACAGTGGAT?CAAATTATGG?GGGTAGGACA?AACAAATCAA
751 800
NC_001463(gag) GCAGCAGCAC?AAGCTAACAT?GGATCAGGCA?AGGCAAATAT?GCCTGCAATG
>AF015181 GCAGCAGCAC?AAGCTAACAT?GGATCAGGCA?AGACAAATAT?GCCTACAATG
801 850
NC_001463(gag) GGTAATAAAT?GCATTAAGAG?CAGTAAGACA?TATGGCGCAC?AGGCCAGGGA
>AF015181 GGTAATAAAC?GCCTTAAGAG?CAGTAAGGCA?TATGGCTCAT?AGGCCAGGGA
851 900
NC_001463(gag) ATCCAATGCT?AGTAAAGCAA?AAAACGAATG?AGCCATATGA?AGATTTTGCA
>AF015181 ATCCAATGCT?AGTAAAGCAA?AAAACAAATG?AGCCATATGA?AGAATTTGCA
901 950
NC_001463(gag) GCAAGACTGC?TAGAAGCAAT?AGATGCAGAG?CCAGTTACAC?AGCCTATAAA
>AF015181 GCAAGACTGC?TAGAAGCAAT?AGATGCAGAA?GCGGTTACAC?AGCCCATAAA
951 1000
NC_001463(gag) AGATTATCTA?AAGCTAACAC?TATCTTATAC?AAATGCATCA?GCAGATTGTC
>AF015181 AGAGTATCTA?AAGCTAACAT?TATCCTATAC?AAATGCAGCC?TCAGATTGTC
1001 1050
NC_001463(gag) AGAAGCAAAT?GGATAGAACA?CTAGGACAAA?GAGTACAACA?AGCTAGTGTA
>AF015181 AAAAGCAAAT?GGAGAGAGTG?CTAGGACAAA?GAGTACAACA?GGCTAGTGTA
1051 1100
NC_001463(gag) GAAGAAAAAA?TGCAAGCATG?TAGAGATGTG?GGATCAGAAG?GGTTCAAAAT
>AF015181 GAAAAAAAAA?TGCAAGCATG?T.........?..........?..........
1101 1150
NC_001463(gag) GCAATTGTTA?GCACAAGCAT?TAAGGCCAGG?AAAAGGAAAA?GGGAATGGAC
>AF015181 ..........?..........?..........?..........?..........
1151 1200
NC_001463(gag) AGCCACAAAG?GTGTTACAAC?TGTGGAAAAC?CGGGACATCA?AGCAAGGCAA
>AF015181 ..........?..........?..........?..........?..........
1201 1250
NC_001463(gag) TGTAGACAAG?GAATCATATG?TCACAACTGT?GGAAAGAGAG?GACATATGCA
>AF015181 ..........?..........?..........?..........?..........
1251 1300
NC_001463(gag) AAAAGAATGC?AGAGGAAAGA?GAGACATAAG?GGGAAAACAG?CAGGGAAACG
>AF015181 ..........?..........?..........?..........?..........
1301 1347
NC_001463(gag) GGAGGAGGGG?GATACGTGTG?GTGCCGTCCG?CTCCTCCTAT?GGAATAA
>AF015181 ..........?..........?..........?.........?........
Table 8
PileUp
MSF：727 Type：N Check：1231 ..
Name：NC_001463(gag720bp)?(SEQ?ID?NO：19)?Len：727 Check：1714 Weight：0
Name：>AF402664?(SEQ?ID?NO：20) Len：727 Check：1659 Weight：0
Name：>AF402665?(SEQ?ID?NO：21) Len：727 Check：331 Weight：0
Name：>AF402666?(SEQ?ID?NO：22) Len：727 Check：7190 Weight：0
Name：>AF402667?(SEQ?ID?NO：23) Len：727 Check：9833 Weight：0
Name：>AF402668?(SEQ?ID?NO：24) Len：727 Check：504 Weight：0
//
1 50
NC_001463(gag720bp) ATGGTGAGTC?TAGATAGAGA?CATGGCGAGG?CAAGTCTCCG?GGGGGAAAAG
>AF402664 ........TC?AAGCAGCAGG?.AGGGAGAAG?CTGGAAAGCA?GTAGACTCAG
>AF402665 ........GC?AAGCAGCAGG?.AGGGAGAAG?CTGGAAAGCA?GTAGACTCAG
>AF402666 ........GC?AAGCAGCAGG?.AGGGAGAAG?CTGGAAAGCA?GTAGACTCAG
>AF402667 ........GC?AAGCAGCAGG?.AGGGAGAAG?CTGGAAAGCA?GTAGACTCAG
>AF402668 ........GC?AAGCAGCAGG?.AGGGAGAAG?CTGGAAAGCA?GTAGACTCAG
51 100
NC_001463(gag720bp) AGATTATCCT?GAGCTCGAAA?AATGTATCAA?GCATGCATGC?AAGATAAAAG
>AF402664 TGATGTTCCA?GCAACTGCAA?AATGTAGCAA?TGCAGCATGG?CCTCGTGTCC
>AF402665 TGATGTTCCA?GCAACTGCAA?AATGTAGCAA?TGCAGCATGG?CCTCGTGTCC
>AF402666 TGATGTTCCA?GCAACTGCAA?AATGTAGCAA?TGCAGCATGG?CCTCGTGTCC
>AF402667 TGATGTTCCA?GCAACTGCAA?AATGTAGCAA?TGCAGCATGG?CCTCGTGTCC
>AF402668 TGATGTTCCA?GCAACTGCAA?AATGTAGCAA?TGCAGCATGG?CCTCGTGTCC
101 150
NC_001463(gag720bp) TTCGACTCAG?AGGGGAGCAC?TTGACAGAAG?GAAATTGTTT?ATGGTGCCTT
>AF402664 GAGGATTTTG?AAAGG..CAG?TTAGTATATT?ATGCTACTAC?CTGGACAAGT
>AF402665 GAGGATTTTG?AAAGG..CAG?TTAGCATATT?ATGCTACTAC?CTGGACAAGT
>AF402666 GAGGATTTTG?AAAGG..CAG?TTGGCATATT?ATGCTACTAC?CTGGACAAGT
>AF402667 GAGGATTTTG?AAAGG..CAG?TTAGCATATT?ATGCTACTAC?CTGGACAAGT
>AF402668 GAGGATTTTG?AAAGG..CAG?TTAGCATATT?ATGCTACTAC?CTGGACAAGT
151 200
NC_001463(gag720bp) AAAACATTAG?ATTACATGTT?TGAGGACCAT?AAAGAGGAAC?CTTGGACAAA
>AF402664 AAAGA..TAT?ATTAGAAGTA?TTGG..CCAT?GATG.....C?CTGGAAATAG
>AF402665 AAAGA..TAT?ATTAGAAGTA?TTGG..CCAT?GATG.....C?CTGGAAATAG
>AF402666 AAAGA..TAT?ATTAGAAGTA?TTGG..CCAT?GATG.....C?CTGGAAACAG
>AF402667 AAAGA..TAT?ATTAGAAGTA?TTGG..CCAT?GATG.....C?CTGGAAATAG
>AF402668 AAAGA..TAT?ATTAGAAGTA?TTGG..CCAT?GATG.....C?CTGGAAATAG
201 250
NC_001463(gag720bp) AGTAAAATTT?AGGACAATAT?GGCAGAAGGT?GAAGAATCTA?ACTCCTGAGG
>AF402664 AGCTCAAAAA?GAGTTAATTC?AAGGGAAATT?GAATGAGGAA?GCAGAAAGGT
>AF402665 AGCTCAAAAA?GAGTTAATTC?AAGGGAAATT?GAATGAGGAA?GCAGAAAGGT
>AF402666 AGCTCAAAAA?GAGTTAATTC?AGGGGAAATT?GAATAAGGAA?GCAGAAAGGT
>AF402667 AGCTCAAAAA?GAGTTAATTC?AAGGGAAATT?GAATGAGGAA?GCAGAAAGGT
>AF402668 AGCTCAAAAA?GAGTTAATTC?AAGGGAAATT?GAATGAGGAA?GCAGAAAGGT
251 300
NC_001463(gag720bp) AGAGTAACAA?AAAAGACTTT?ATGTCTTTGC?AGGCCACATT?AGCGGGTCTA
>AF402664 GGAG.AAGAA?ATAATCCACC?ACCTCAA.GC?AGGCG.....?.GAGGATTAA
>AF402665 GGAG.AAGGA?ATAATCCACC?ACCTCAA.GC?AGGCG.....?.GAGGATTAA
>AF402666 GGAG.AAGGA?ATAATCCACC?ACCTCAA.GC?ACAAG.....?.GAGGATTAA
>AF402667 GGAG.AAGGA?ATAATCCACC?ACCTCAA.GC?AGGCG.....?.GAGGATTAA
>AF402668 GGAG.AAGGA?ATAATCCACC?ACCTCAA.GC?AGGCG.....?.GAGGATTAA
301 350
NC_001463(gag720bp) ATGTGTTGCC?AA..ATGGGG?ATGAGACCTG?AGACATTGCA?AGATGCAATG
>AF402664 CAGTGGATCA?AATTATGGGG?GTAGGACAAA?CAAATCAAGC?AGCGGCACAG
>AF402665 CAGTGGATCA?AATTATGGGG?GTAGGACAAA?CAAATCAAGC?AGCGGCACAG
>AF402666 CAGTGGATCA?AATTATGGGG?GTAGGACAAA?CAAATCAGGC?AGCGGCACAG
>AF402667 CAGTGGATCA?AATTATGGGG?GTAGGACAAA?CAAATCAAGC?AGCGGCACAG
>AF402668 CAGTGGATCA?AATTATGGGG?GTAGGACAAA?CAAATCAAGC?AGCGGCACAG
351 400
NC_001463(gag720bp) GCTA.CAGTA?ATCATGAAAG?ATGGGTT..A?CTGGAACAAG?.AGGAAAAGA
>AF402664 GCTAACATGG?ATCAGGCAAG?ACAAATATGT?CTGCAATGGG?TAATAACAGC
>AF402665 GCTAACATGG?ATCAGGCAAG?ACAAATATGC?CTGCAATGGG?TAATAACAGC
>AF402666 GCTAACATGG?ATCAGGCAAG?ACAAATATGC?CTGCAATGGG?TAATAACAGC
>AF402667 GCTAACATGG?ATCAGGCAAG?ACAAATATGC?CTGCAATGGG?TAATAACAGC
>AF402668 GCTAACATGG?ATCAGGCAAG?ACAAATATGC?CTGCAATGGG?TAATAACAGC
401 450
NC_001463(gag720bp) AGGAAGACAA?AAGAGAAAAG?GAAGAGAGTG?TCTTCCCAAT?AGTAGTGCAA
>AF402664 ACTAAGAGCA?GTGAGACATA?TGGCTCACAA?ACCAGGGAAT?CCAA.TGCTA
>AF402665 ACTAAGAGCA?GTGAGACATA?TGGCTCACAA?ACCAGGGAAT?CCAA.TGCTA
>AF402666 ACTAAGAGCA?GTGAGACATA?TGGCTCACAA?ACCAGGGAAT?CCAA.TGCTA
>AF402667 ACTAAGAGCA?GTGAGACATA?TGGCTCACAA?ACCAGGGAAT?CCAA.TGCTA
>AF402668 ACTAAGAGCA?GTGAGACATA?TGGCTCACAA?ACCAGGGAAT?CCAA.TGCTA
451 500
NC_001463(gag720bp) GCAGCAGGAG?GGAGAAGCTG?GAAAGCAGTA?GATTCTGTAA?TGTTCCAGCA
>AF402664 GTAAAGCAAA?AAACAAATGA?GTCATATGAA?GATTTTGCCG?CAAGACTGCT
>AF402665 GTAAAGCAAA?AGACAAATGA?GTCATATGAA?GATTTTGCCG?CAAGACTGCT
>AF402666 GTAAAGCAAA?AGACAAATGA?GTCATATGAA?GATTTTGCCG?CAAGACTGCT
>AF402667 GTAAAGCAAA?AGACAAATGA?GTCATATGAA?GATTTTGCCG?CAAGACTGCT
>AF402668 GTAAAGCAAA?AGACAAATGA?GTCATATGAA?AAATTTTCAG?CAAGACTCCT
501 550
NC_001463(gag720bp) ACTGCAAACA?GTAGCA.ATG?CAGCATGGCC?TCGTGTCTGA?GGACTTTGAA
>AF402664 AGAAGCAATA?GATGCAGAAC?CAGTTACACA?GCAAATAAAA?GAATATTTAA
>AF402665 AGAAGCAATA?GATGCAGAAC?CAGTTACACA?GCAAATAAAA?GAATATTTAA
>AF402666 AGAAGCAATA?GATGCAGAAC?CAGTTACACA?GCAAATAAAA?GAATATTTAA
>AF402667 AGAAGCAATA?GATGCAGAAC?CAGTTACACA?GCAAATAAA.?GAATATTTAA
>AF402668 AGAAGCAATA?GATGCAGAAC?CAGTTACACA?GCCTATAAAA?GAATATTTAA
551 600
NC_001463(gag720bp) AGGCAGTTGG?CATATTATGC?TACTACCTGG?ACAAGTAAAG?ACATACTAGA
>AF402664 AGTTA.....?.ACATTATCT?TAC.ACAAAT?GCATCCTCAG?ACTGTCAGAA
>AF402665 AGTTA.....?.ACATTATCT?TAC.ACAAAT?GCATCCTCAG?ACTGTCAAAA
>AF402666 AGTTA.....?.ACATTATCT?TAC.ACAAAT?GCATCCTCAG?ACTGTCAGAA
>AF402667 ..........?..........?..........?..........?..........
>AF402668 AGTTA.....?.ACATTATCT?TAC.ACAAAT?GCATCCTCAG?ACTGTCAAAA
601 650
NC_001463(gag720bp) AGTATTGGCC?ATGATGCCTG?GAAATAGAGC?TCAAAAGGAG?TTAATTCAAG
>AF402664 ACAGATGGAT?AGAGTACTAG?GACAGAGAGT?GCAACAAGCT?AGTGTGGAAG
>AF402665 ACAAATGGAT?AGAATACTAG?GACAGAGAGT?GCAACAAGCT?AGTGTGGAAG
>AF402666 ACAAATGGAT?AGAGTACTAG?GACAGAGAGT?GCAACAAGCT?AGTGTGGAAG
>AF402667 ..........?..........?..........?..........?..........
>AF402668 ACAAATGGAT?AGAGTACTAG?GACAGAGAGT?GCAACAAGCT?AGTGTGGAAG
651 700
NC_001463(gag720bp) GGAAATTAAA?TGAAGAAGCA?GAAAGGTGGA?GAAGGAATAA?TCCACCACCT
>AF402664 AAAAAATGCA?AGCAT..GCA?GAGATGTGGG?ATCAGAAGGA?TTCAGAATGC
>AF402665 AAAAAATGCA?AGCAT..GCA?GAGATGTGGG?ATCAGAAGGG?TTCAGAATGC
>AF402666 AAAAAATGCA?AGCAT..GCA?GAGATGTGGG?ATCAGAAGG.?..........
>AF402667 ..........?..........?..........?..........?..........
>AF402668 AAAAAATGCA?AGCAT..GCA?GAGATGTGGG?ATCAGAAGGA?TTCAGAATGC
701 727
NC_001463(gag720bp) CCAGCAGGAG?GAGGATTAAC?AGTGGAT
>AF402664 ..........?..........?.......
>AF402665 ..........?..........?.......
>AF402666 ..........?..........?.......
>AF402667 ..........?..........?.......
>AF402668 ..........?..........?.......
PileUp
MSF：1347 Type：N Check：5320 ..
Name：NC_001463(gag)(SEQ?ID?NO：25) Len：1347?Check：6959?Weight：0
Name：>AF402664?(SEQ?ID?NO：26)?Len：1347 Check：1590 Weight：0
Name：>AF402665?(SEQ?ID?NO：27)?Len：1347 Check：9222 Weight：0
Name：>AF402666?(SEQ?ID?NO：28)?Len：1347 Check：4950 Weight：0
Name：>AF402667?(SEQ?ID?NO：29)?Len：1347 Check：3156 Weight：0
Name：>AF402668?(SEQ?ID?NO：30)?Len：1347 Check：9443 Weight：0
//
1 50
NC_001463(gag) ATGGTGAGTC?TAGATAGAGA?CATGGCGAGG?CAAGTCTCCG?GGGGGAAAAG
>AF402664 ..........?..........?..........?..........?..........
>AF402665 ..........?..........?..........?..........?..........
>AF402666 ..........?..........?..........?..........?..........
>AF402667 ..........?..........?..........?..........?..........
>AF402668 ..........?..........?..........?..........?..........
51 100
NC_001463(gag) AGATTATCCT?GAGCTCGAAA?AATGTATCAA?GCATGCATGC?AAGATAAAAG
>AF402664 ..........?..........?..........?..........?..........
>AF402665 ..........?..........?..........?..........?..........
>AF402666 ..........?..........?..........?..........?..........
>AF402667 ..........?..........?..........?..........?..........
>AF402668 ..........?..........?..........?..........?..........
101 150
NC_001463(gag) TTCGACTCAG?AGGGGAGCAC?TTGACAGAAG?GAAATTGTTT?ATGGTGCCTT
>AF402664 ..........?..........?..........?..........?..........
>AF402665 ..........?..........?..........?..........?..........
>AF402666 ..........?..........?..........?..........?..........
>AF402667 ..........?..........?..........?..........?..........
>AF402668 ..........?..........?..........?..........?..........
151 200
NC_001463(gag) AAAACATTAG?ATTACATGTT?TGAGGACCAT?AAAGAGGAAC?CTTGGACAAA
>AF402664 ..........?..........?..........?..........?..........
>AF402665 ..........?..........?..........?..........?..........
>AF402666 ..........?..........?..........?..........?..........
>AF402667 ..........?..........?..........?..........?..........
>AF402668 ..........?..........?..........?..........?..........
201 250
NC_001463(gag) AGTAAAATTT?AGGACAATAT?GGCAGAAGGT?GAAGAATCTA?ACTCCTGAGG
>AF402664 ..........?..........?..........?..........?..........
>AF402665 ..........?..........?..........?..........?..........
>AF402666 ..........?..........?..........?..........?..........
>AF402667 ..........?..........?..........?..........?..........
>AF402668 ..........?..........?..........?..........?..........
251 300
NC_001463(gag) AGAGTAACAA?AAAAGACTTT?ATGTCTTTGC?AGGCCACATT?AGCGGGTCTA
>AF402664 ..........?..........?..........?..........?..........
>AF402665 ..........?..........?..........?..........?..........
>AF402666 ..........?..........?..........?..........?..........
>AF402667 ..........?..........?..........?..........?..........
>AF402668 ..........?..........?..........?..........?..........
301 350
NC_001463(gag) ATGTGTTGCC?AAATGGGGAT?GAGACCTGAG?ACATTGCAAG?ATGCAATGGC
>AF402664 ..........?..........?..........?..........?..........
>AF402665 ..........?..........?..........?..........?..........
>AF402666 ..........?..........?..........?..........?..........
>AF402667 ..........?..........?..........?..........?..........
>AF402668 ..........?..........?..........?..........?..........
351 400
NC_001463(gag) TACAGTAATC?ATGAAAGATG?GGTTACTGGA?ACAAGAGGAA?AAGAAGGAAG
>AF402664 ..........?..........?..........?..........?..........
>AF402665 ..........?..........?..........?..........?..........
>AF402666 ..........?..........?..........?..........?..........
>AF402667 ..........?..........?..........?..........?..........
>AF402668 ..........?..........?..........?..........?..........
401 450
NC_001463(gag) ACAAAAGAGA?AAAGGAAGAG?AGTGTCTTCC?CAATAGTAGT?GCAAGCAGCA
>AF402664 ..........?..........?..........?..........?TCAAGCAGCA
>AF402665 ..........?..........?..........?..........?GCAAGCAGCA
>AF402666 ..........?..........?..........?..........?GCAAGCAGCA
>AF402667 ..........?..........?..........?..........?GCAAGCAGCA
>AF402668 ..........?..........?..........?..........?GCAAGCAGCA
451 500
NC_001463(gag) GGAGGGAGAA?GCTGGAAAGC?AGTAGATTCT?GTAATGTTCC?AGCAACTGCA
>AF402664 GGAGGGAGAA?GCTGGAAAGC?AGTAGACTCA?GTGATGTTCC?AGCAACTGCA
>AF402665 GGAGGGAGAA?GCTGGAAAGC?AGTAGACTCA?GTGATGTTCC?AGCAACTGCA
>AF402666 GGAGGGAGAA?GCTGGAAAGC?AGTAGACTCA?GTGATGTTCC?AGCAACTGCA
>AF402667 GGAGGGAGAA?GCTGGAAAGC?AGTAGACTCA?GTGATGTTCC?AGCAACTGCA
>AF402668 GGAGGGAGAA?GCTGGAAAGC?AGTAGACTCA?GTGATGTTCC?AGCAACTGCA
501 550
NC_001463(gag) AACAGTAGCA?ATGCAGCATG?GCCTCGTGTC?TGAGGACTTT?GAAAGGCAGT
>AF402664 AAATGTAGCA?ATGCAGCATG?GCCTCGTGTC?CGAGGATTTT?GAAAGGCAGT
>AF402665 AAATGTAGCA?ATGCAGCATG?GCCTCGTGTC?CGAGGATTTT?GAAAGGCAGT
>AF402666 AAATGTAGCA?ATGCAGCATG?GCCTCGTGTC?CGAGGATTTT?GAAAGGCAGT
>AF402667 AAATGTAGCA?ATGCAGCATG?GCCTCGTGTC?CGAGGATTTT?GAAAGGCAGT
>AF402668 AAATGTAGCA?ATGCAGCATG?GCCTCGTGTC?CGAGGATTTT?GAAAGGCAGT
551 600
NC_001463(gag) TGGCATATTA?TGCTACTACC?TGGACAAGTA?AAGACATACT?AGAAGTATTG
>AF402664 TAGTATATTA?TGCTACTACC?TGGACAAGTA?AAGATATATT?AGAAGTATTG
>AF402665 TAGCATATTA?TGCTACTACC?TGGACAAGTA?AAGATATATT?AGAAGTATTG
>AF402666 TGGCATATTA?TGCTACTACC?TGGACAAGTA?AAGATATATT?AGAAGTATTG
>AF402667 TAGCATATTA?TGCTACTACC?TGGACAAGTA?AAGATATATT?AGAAGTATTG
>AF402668 TAGCATATTA?TGCTACTACC?TGGACAAGTA?AAGATATATT?AGAAGTATTG
601 650
NC_001463(gag)?GCCATGATGC?CTGGAAATAG?AGCTCAAAAG?GAGTTAATTC?AAGGGAAATT
>AF402664 GCCATGATGC?CTGGAAATAG?AGCTCAAAAA?GAGTTAATTC?AAGGGAAATT
>AF402665 GCCATGATGC?CTGGAAATAG?AGCTCAAAAA?GAGTTAATTC?AAGGGAAATT
>AF402666 GCCATGATGC?CTGGAAACAG?AGCTCAAAAA?GAGTTAATTC?AGGGGAAATT
>AF402667 GCCATGATGC?CTGGAAATAG?AGCTCAAAAA?GAGTTAATTC?AAGGGAAATT
>AF402668 GCCATGATGC?CTGGAAATAG?AGCTCAAAAA?GAGTTAATTC?AAGGGAAATT
651 700
NC_001463(gag) AAATGAAGAA?GCAGAAAGGT?GGAGAAGGAA?TAATCCACCA?CCTCCAGCAG
>AF402664 GAATGAGGAA?GCAGAAAGGT?GGAGAAGAAA?TAATCCACCA?CCTCAAGCAG
>AF402665 GAATGAGGAA?GCAGAAAGGT?GGAGAAGGAA?TAATCCACCA?CCTCAAGCAG
>AF402666 GAATAAGGAA?GCAGAAAGGT?GGAGAAGGAA?TAATCCACCA?CCTCAAGCAC
>AF402667 GAATGAGGAA?GCAGAAAGGT?GGAGAAGGAA?TAATCCACCA?CCTCAAGCAG
>AF402668 GAATGAGGAA?GCAGAAAGGT?GGAGAAGGAA?TAATCCACCA?CCTCAAGCAG
701 750
NC_001463(gag) GAGGAGGATT?AACAGTGGAT?CAAATTATGG?GGGTAGGACA?AACAAATCAA
>AF402664 GCGGAGGATT?AACAGTGGAT?CAAATTATGG?GGGTAGGACA?AACAAATCAA
>AF402665 GCGGAGGATT?AACAGTGGAT?CAAATTATGG?GGGTAGGACA?AACAAATCAA
>AF402666 AAGGAGGATT?AACAGTGGAT?CAAATTATGG?GGGTAGGACA?AACAAATCAG
>AF402667 GCGGAGGATT?AACAGTGGAT?CAAATTATGG?GGGTAGGACA?AACAAATCAA
>AF402668 GCGGAGGATT?AACAGTGGAT?CAAATTATGG?GGGTAGGACA?AACAAATCAA
751 800
NC_001463(gag) GCAGCAGCAC?AAGCTAACAT?GGATCAGGCA?AGGCAAATAT?GCCTGCAATG
>AF402664 GCAGCGGCAC?AGGCTAACAT?GGATCAGGCA?AGACAAATAT?GTCTGCAATG
>AF402665 GCAGCGGCAC?AGGCTAACAT?GGATCAGGCA?AGACAAATAT?GCCTGCAATG
>AF402666 GCAGCGGCAC?AGGCTAACAT?GGATCAGGCA?AGACAAATAT?GCCTGCAATG
>AF402667 GCAGCGGCAC?AGGCTAACAT?GGATCAGGCA?AGACAAATAT?GCCTGCAATG
>AF402668 GCAGCGGCAC?AGGCTAACAT?GGATCAGGCA?AGACAAATAT?GCCTGCAATG
801 850
NC_001463(gag) GGTAATAAAT?GCATTAAGAG?CAGTAAGACA?TATGGCGCAC?AGGCCAGGGA
>AF402664 GGTAATAACA?GCACTAAGAG?CAGTGAGACA?TATGGCTCAC?AAACCAGGGA
>AF402665 GGTAATAACA?GCACTAAGAG?CAGTGAGACA?TATGGCTCAC?AAACCAGGGA
>AF402666 GGTAATAACA?GCACTAAGAG?CAGTGAGACA?TATGGCTCAC?AAACCAGGGA
>AF402667 GGTAATAACA?GCACTAAGAG?CAGTGAGACA?TATGGCTCAC?AAACCAGGGA
>AF402668 GGTAATAACA?GCACTAAGAG?CAGTGAGACA?TATGGCTCAC?AAACCAGGGA
851 900
NC_001463(gag) ATCCAATGCT?AGTAAAGCAA?AAAACGAATG?AGCCATATGA?AGATTTTGCA
>AF402664 ATCCAATGCT?AGTAAAGCAA?AAAACAAATG?AGTCATATGA?AGATTTTGCC
>AF402665 ATCCAATGCT?AGTAAAGCAA?AAGACAAATG?AGTCATATGA?AGATTTTGCC
>AF402666 ATCCAATGCT?AGTAAAGCAA?AAGACAAATG?AGTCATATGA?AGATTTTGCC
>AF402667 ATCCAATGCT?AGTAAAGCAA?AAGACAAATG?AGTCATATGA?AGATTTTGCC
>AF402668 ATCCAATGCT?AGTAAAGCAA?AAGACAAATG?AGTCATATGA?AAAATTTTCA
901 950
NC_001463(gag) GCAAGACTGC?TAGAAGCAAT?AGATGCAGAG?CCAGTTACAC?AGCCTATAAA
>AF402664 GCAAGACTGC?TAGAAGCAAT?AGATGCAGAA?CCAGTTACAC?AGCAAATAAA
>AF402665 GCAAGACTGC?TAGAAGCAAT?AGATGCAGAA?CCAGTTACAC?AGCAAATAAA
>AF402666 GCAAGACTGC?TAGAAGCAAT?AGATGCAGAA?CCAGTTACAC?AGCAAATAAA
>AF402667 GCAAGACTGC?TAGAAGCAAT?AGATGCAGAA?CCAGTTACAC?AGCAAATAAA
>AF402668 GCAAGACTCC?TAGAAGCAAT?AGATGCAGAA?CCAGTTACAC?AGCCTATAAA
951 1000
NC_001463(gag) AGATTATCTA?AAGCTAACAC?TATCTTATAC?AAATGCATCA?GCAGATTGTC
>AF402664 AGAATATTTA?AAGTTAACAT?TATCTTACAC?AAATGCATCC?TCAGACTGTC
>AF402665 AGAATATTTA?AAGTTAACAT?TATCTTACAC?AAATGCATCC?TCAGACTGTC
>AF402666 AGAATATTTA?AAGTTAACAT?TATCTTACAC?AAATGCATCC?TCAGACTGTC
>AF402667 .GAATATTTA?A.........?..........?..........?..........
>AF402668 AGAATATTTA?AAGTTAACAT?TATCTTACAC?AAATGCATCC?TCAGACTGTC
1001 1050
NC_001463(gag) AGAAGCAAAT?GGATAGAACA?CTAGGACAAA?GAGTACAACA?AGCTAGTGTA
>AF402664 AGAAACAGAT?GGATAGAGTA?CTAGGACAGA?GAGTGCAACA?AGCTAGTGTG
>AF402665 AAAAACAAAT?GGATAGAATA?CTAGGACAGA?GAGTGCAACA?AGCTAGTGTG
>AF402666 AGAAACAAAT?GGATAGAGTA?CTAGGACAGA?GAGTGCAACA?AGCTAGTGTG
>AF402667 ..........?..........?..........?..........?..........
>AF402668 AAAAACAAAT?GGATAGAGTA?CTAGGACAGA?GAGTGCAACA?AGCTAGTGTG
1051 1100
NC_001463(gag) GAAGAAAAAA?TGCAAGCATG?TAGAGATGTG?GGATCAGAAG?GGTTCAAAAT
>AF402664 GAAGAAAAAA?TGCAAGCATG?CAGAGATGTG?GGATCAGAAG?GATTCAGAAT
>AF402665 GAAGAAAAAA?TGCAAGCATG?CAGAGATGTG?GGATCAGAAG?GGTTCAGAAT
>AF402666 GAAGAAAAAA?TGCAAGCATG?CAGAGATGTG?GGATCAGAAG?G.........
>AF402667 ..........?..........?..........?..........?..........
>AF402668 GAAGAAAAAA?TGCAAGCATG?CAGAGATGTG?GGATCAGAAG?GATTCAGAAT
1101 1150
NC_001463(gag) GCAATTGTTA?GCACAAGCAT?TAAGGCCAGG?AAAAGGAAAA?GGGAATGGAC
>AF402664 GC........?..........?..........?..........?..........
>AF402665 GC........?..........?..........?..........?..........
>AF402666 ..........?..........?..........?..........?..........
>AF402667 ..........?..........?..........?..........?..........
>AF402668 GC........?..........?..........?..........?..........
1151 1200
NC_001463(gag) AGCCACAAAG?GTGTTACAAC?TGTGGAAAAC?CGGGACATCA?AGCAAGGCAA
>AF402664 ..........?..........?..........?..........?..........
>AF402665 ..........?..........?..........?..........?..........
>AF402666 ..........?..........?..........?..........?..........
>AF402667 ..........?..........?..........?..........?..........
>AF402668 ..........?..........?..........?..........?..........
1201 1250
NC_001463(gag) TGTAGACAAG?GAATCATATG?TCACAACTGT?GGAAAGAGAG?GACATATGCA
>AF402664 ..........?..........?..........?..........?..........
>AF402665 ..........?..........?..........?..........?..........
>AF402666 ..........?..........?..........?..........?..........
>AF402667 ..........?..........?..........?..........?..........
>AF402668 ..........?..........?..........?..........?..........
1251 1300
NC_001463(gag) AAAAGAATGC?AGAGGAAAGA?GAGACATAAG?GGGAAAACAG?CAGGGAAACG
>AF402664 ..........?..........?..........?..........?..........
>AF402665 ..........?..........?..........?..........?..........
>AF402666 ..........?..........?..........?..........?..........
>AF402667 ..........?..........?..........?..........?..........
>AF402668 ..........?..........?..........?..........?..........
1301 1347
NC_001463(gag) GGAGGAGGGG?GATACGTGTG?GTGCCGTCCG?CTCCTCCTAT?GGAATAA
>AF402664 ..........?..........?..........?..........?.......
>AF402665 ..........?..........?..........?..........?.......
>AF402666 ..........?..........?..........?..........?.......
>AF402667 ..........?..........?..........?..........?.......
>AF402668 ..........?..........?..........?..........?.......
Table 9
PileUp
MSF：742 Type：N Check：6523 ..
Name：NC_001463(gag720bp)?(SEQ?ID?NO：31) Len：742 Check：3818 Weight：0
Name：>AJ305040?(SEQ?ID?NO：32) Len：742 Check：1263 Weight：0
Name：>AJ305041?(SEQ?ID?NO：33) Len：742 Check：9126 Weight：0
Name：>AJ305042?(SEQ?ID?NO：34) Len：742 Check：2316 Weight：0
//
1 50
NC_001463(gag720bp) ATGGTGAGTC?TAGATAGAGA?CATGGCGAGG?CAAGTCTC..?CGGGGGGAAA
>AJ305040 ......GCAG?TCGATGCTGT?AATGTTCCAG?CAAATGCAAA?CAGTAGCCAT
>AJ305041 ......GCAG?TAGACTCAGT?AATGTTCCAG?CAACTGCAAA?CAGTAGCAAT
>AJ305042 ......GCAG?TCGATGCTGT?AATGTTCCAG?CAAATGCAAA?CAGTAGCCAT
51 100
NC_001463(gag720bp) AGAGATTATC?CTGAGCTCGA?AAAATGTATC?AAGCATGCAT?GCAAGATAAA
>AJ305040 GCAGCATGGT?CTTGTGTCTG?AGGACTTTGA?AAGGCAGTTA?GCAT.ATTGT
>AJ305041 GCAGCATGGC?CTCGTGTCCG?AGGATTTTGA?AAGGCAGTTG?GCAT.ATTAT
>AJ305042 GCAGCATGGT?CTTGTGTCTG?AGGACTTTGA?AAGGCAGTTA?GCAT.ATTAT
101 150
NC_001463(gag720bp) AGTTCGACTC?AGAGGGG..A?GCACTTGACA?GAAGGAAATT?GTTTATGGTG
>AJ305040 GCTACTACCT?GGACAAGTAA?AGATATATTA?GAAGTA..TT?GGCCATGATG
>AJ305041 GCTACTACCT?GGACGAGTAA?AGACATACTA?GAAGTA..TT?GGCCATGATG
>AJ305042 GCTACTACCT?GGACAAGTAA?AGATATATTA?GAAGTA..TT?GGCCATGATG
151 200
NC_001463(gag720bp) CCTTAAAACA?TTAGATTACA?TGTTTGAGGA?CCATAAAGAG?GAACCTTGGA
>AJ305040 CCTGGAAATA?G.AGCTCAAA?AA...GAGTT?AATTCAAG.G?AAAATTAAAC
>AJ305041 CCTGGAAACA?G.AGCTCAAA?AG...GAGTT?AATTCAAG.G?GAAATTAAAT
>AJ305042 CCTGGAAATA?G.AGCTCAAA?AA...GAGTT?AATTCAAG.G?AAAATTAAAT
201 250
NC_001463(gag720bp) CAAAAGTAAA?ATTTAGGACA?ATATGGCAGA?AGGTGAAGAA?TCTAACTCCT
>AJ305040 GAGGAAGCAG?AA..AGGTGG?AGAAGGAATA?A..TCCACCG?CCTCCACAAG
>AJ305041 GAAGAGGCAG?AA..AGGTGG?AGAAGACATA?A..TCCACCC?CCTCCGGCGG
>AJ305042 GAGGAAGCAG?AA..AGGTGG?AGAAGGAATA?A..TCCACCG?CCTCCACAGG
251 300
NC_001463(gag720bp) GAGGAG.AGT?AACAAAAAAG?.ACTTTATGT?CTTTGCAGGC?CACATTAGCG
>AJ305040 GAGGGGGATT?AACAGTGGAT?CAAATTATGG?GGAT..AGGA?CAAACAAATC
>AJ305041 GAGGAGGATT?AACAGTGGAT?CAAATTATGG?GGGT..AGGA?CAAACAAATC
>AJ305042 GAGGGGGATT?AACAGTGGAT?CAAATTATGG?GGAT..AGGA?CAAACAAATC
301 350
NC_001463(gag720bp) GGTCTAATGT?GTTGCCAAAT?GGGGATGAGA?CCTGAGACAT?.....TGCAA
>AJ305040 AAGCAGCAGC?ACAAGCTAAC?ATGGATCAGG?CAAGACACAT?ATGCCTGCAA
>AJ305041 AAGCAGCAGC?ACAAGCTAAC?ATGGATCAGG?CAAGACAAAT?ATGCCTGCAA
>AJ305042 AAGCAGCAGC?ACAAGCTAAC?ATGGATCAGG?CAAGACACAT?ATGCCTGCAA
351 400
NC_001463(gag720bp) GATGCAATGG?CTACAGTAAT?..CA.TGAAA?GATGGGTTAC?TGGAACAAGA
>AJ305040 TGGGTAATAA?CAGCATTAAG?AGCAGTAAGA?CATATGGCTC?ACAGACCAGG
>AJ305041 TGGGTAATAA?CAGCATTAAG?AGCAGTGAGG?TATATGACTC?ACAAACCAGG
>AJ305042 TGGGTAATAA?CAGCATTAAG?AGCAGTAAGA?CATATGGCTC?ACAGACCAGG
401 450
NC_001463(gag720bp) GGA...AAAG?A.AGGAAGAC?AAAAGAGAAA?AGGAAGAGAG?T..GTCTTCC
>AJ305040 GAATCCAATG?CTCGTAAAAC?AAAAAACAAA?TGAGCCATAT?GAAGAGTTTG
>AJ305041 GAATCCAATG?CTAGTAAAAC?AAAAAACAAA?TGAAGCATAT?GAAGAGTTTA
>AJ305042?GAATCCAATG?CTCGTAAAAC?AAAAAACAAA?TGAGCCATAT?GAAGAGTTTG
451 500
NC_001463(gag720bp)?CAATAGTAGT?GCAAGCAGCA?GGAG..GGAG?AAGCTGGAAA?GCAGTAGATT
>AJ305040?CAGCAAAACT?ATTAGAAGCA?ATAGATGCAG?AACCAGTAAC?ACAGCCCATA
>AJ305041?CAGCGAGACT?GCTAGAAGCA?ATAGATGCAG?AGCCAGTAAC?ACAGCCCACA
>AJ305042?CAGCAAAACT?ATTAGAAGCA?ATAGATGCAG?AACCAGTAAC?ACAGCTCATA
501 550
NC_001463(gag720bp)?CTGTAATGTT?CCAGCAACTG?CAAACAGTAG?CAATGCAGCA?TGGCCTCGTG
>AJ305040?AAAGACTAT.?.CTAAAGTT.?..AACATTAT?CT.TATACAA?ATGCGTC...
>AJ305041?AAAGAATAT.?.CTAAAACT.?..AACATTAT?CT.TATACAA?ATGCATC...
>AJ305042?AAAGACTAT.?.CTAAAGTT.?..AACATTAT?CT.TATACAA?ATGCGTC...
551 600
NC_001463(gag720bp)?TCTGAGGACT?TTGAAAGGCA?GTTGGCATAT?TATGCTACTA?CCTGGACAAG
>AJ305040?.CTCAG.ACT?GTCAAAAGCA?AATGG.ATAG?AGTGCTGGGA?CAAAG...AG
>AJ305041?.CTCAG.ACT?GTCAAAAGCA?AATGG.ATAG?AGTACTAGGA?CAAAG...AG
>AJ305042?.CTCAG.ACT?GTCAAAAGCA?AATGG.ATAG?AGTGCTGGGA?CAAAG...AG
601 650
NC_001463(gag720bp)?TAAAGACATA?CTAGAAGTAT?TGGCCATGAT?GCCTGGAAAT?AGAGCTCAAA
>AJ305040?TGCA.ACAAG?CTAGT.GTAG?ACGAGAAAAT?GCAA......?..........
>AJ305041?TGCA.ACAAG?CTAGT.GTAG?AAGAAAAAAT?GCAA......?..........
>AJ305042?TGCA.ACAAG?CTAGT.GTAG?ACGAGAAGAT?GCAA......?..........
651 700
NC_001463(gag720bp)?AGGAGTTAAT?TCAAGGGAAA?TTAAATGAAG?AAGCAGAAAG?GTGGAGAAGG
>AJ305040?..........?..........?..........?..........?..........
>AJ305041?..........?..........?..........?..........?..........
>AJ305042?..........?..........?..........?..........?..........
701 742
NC_001463(gag720bp)?AATAATCCAC?CACCTCCAGC?AGGAGGAGGA?TTAACAGTGG?AT
>AJ305040?..........?..........?..........?..........?..
>AJ305041?..........?..........?..........?..........?..
>AJ305042?..........?..........?..........?..........?..
PileUp
MSF：1347 Type：N Check：9510 ..
Name：NC_001463(gag)?(SEQ?ID?NO：35)?Len：1347?Check：6959 Weight：0
Name：>AJ305040?(SEQ?ID?NO：36)?Len：1347 Check：1930 Weight：0
Name：>AJ305041?(SEQ?ID?NO：37)?Len：1347 Check：7682 Weight：0
Name：>AJ305042?(SEQ?ID?NO：38)?Len：1347 Check：2939 Weight：0
//
1 50
NC_001463(gag) ATGGTGAGTC?TAGATAGAGA?CATGGCGAGG?CAAGTCTCCG?GGGGGAAAAG
>AJ305040 ..........?..........?..........?..........?..........
>AJ305041 ..........?..........?..........?..........?..........
>AJ305042 ..........?..........?..........?..........?..........
51 100
NC_001463(gag) AGATTATCCT?GAGCTCGAAA?AATGTATCAA?GCATGCATGC?AAGATAAAAG
>AJ305040 ..........?..........?..........?..........?..........
>AJ305041 ..........?..........?..........?..........?..........
>AJ305042 ..........?..........?..........?..........?..........
101 150
NC_001463(gag) TTCGACTCAG?AGGGGAGCAC?TTGACAGAAG?GAAATTGTTT?ATGGTGCCTT
>AJ305040 ..........?..........?..........?..........?..........
>AJ305041?..........?..........?..........?..........?..........
>AJ305042?..........?..........?..........?..........?..........
151 200
NC_001463(gag)?AAAACATTAG?ATTACATGTT?TGAGGACCAT?AAAGAGGAAC?CTTGGACAAA
>AJ305040?..........?..........?..........?..........?..........
>AJ305041?..........?..........?..........?..........?..........
>AJ305042?..........?..........?..........?..........?..........
201 250
NC_001463(gag)?AGTAAAATTT?AGGACAATAT?GGCAGAAGGT?GAAGAATCTA?ACTCCTGAGG
>AJ305040?..........?..........?..........?..........?..........
>AJ305041?..........?..........?..........?..........?..........
>AJ305042?..........?..........?..........?..........?..........
251 300
NC_001463(gag)?AGAGTAACAA?AAAAGACTTT?ATGTCTTTGC?AGGCCACATT?AGCGGGTCTA
>AJ305040?..........?..........?..........?..........?..........
>AJ305041?..........?..........?..........?..........?..........
>AJ305042?..........?..........?..........?..........?..........
301 350
NC_001463(gag)?ATGTGTTGCC?AAATGGGGAT?GAGACCTGAG?ACATTGCAAG?ATGCAATGGC
>AJ305040?..........?..........?..........?..........?..........
>AJ305041?..........?..........?..........?..........?..........
>AJ305042?..........?..........?..........?..........?..........
351 400
NC_001463(gag)?TACAGTAATC?ATGAAAGATG?GGTTACTGGA?ACAAGAGGAA?AAGAAGGAAG
>AJ305040?..........?..........?..........?..........?..........
>AJ305041?..........?..........?..........?..........?..........
>AJ305042?..........?..........?..........?..........?..........
401 450
NC_001463(gag)?ACAAAAGAGA?AAAGGAAGAG?AGTGTCTTCC?CAATAGTAGT?GCAAGCAGCA
>AJ305040?..........?..........?..........?..........?..........
>AJ305041?..........?..........?..........?..........?..........
>AJ305042?..........?..........?..........?..........?..........
451 500
NC_001463(gag)?GGAGGGAGAA?GCTGGAAAGC?AGTAGATTCT?GTAATGTTCC?AGCAACTGCA
>AJ305040?..........?........GC?AGTCGATGCT?GTAATGTTCC?AGCAAATGCA
>AJ305041?..........?........GC?AGTAGACTCA?GTAATGTTCC?AGCAACTGCA
>AJ305042?..........?........GC?AGTCGATGCT?GTAATGTTCC?AGCAAATGCA
501 550
NC_001463(gag)?AACAGTAGCA?ATGCAGCATG?GCCTCGTGTC?TGAGGACTTT?GAAAGGCAGT
>AJ305040?AACAGTAGCC?ATGCAGCATG?GTCTTGTGTC?TGAGGACTTT?GAAAGGCAGT
>AJ305041?AACAGTAGCA?ATGCAGCATG?GCCTCGTGTC?CGAGGATTTT?GAAAGGCAGT
>AJ305042?AACAGTAGCC?ATGCAGCATG?GTCTTGTGTC?TGAGGACTTT?GAAAGGCAGT
551 600
NC_001463(gag)?TGGCATATTA?TGCTACTACC?TGGACAAGTA?AAGACATACT?AGAAGTATTG
>AJ305040?TAGCATATTG?TGCTACTACC?TGGACAAGTA?AAGATATATT?AGAAGTATTG
>AJ305041?TGGCATATTA?TGCTACTACC?TGGACGAGTA?AAGACATACT?AGAAGTATTG
>AJ305042?TAGCATATTA?TGCTACTACC?TGGACAAGTA?AAGATATATT?AGAAGTATTG
601 650
NC_001463(gag)?GCCATGATGC?CTGGAAATAG?AGCTCAAAAG?GAGTTAATTC?AAGGGAAATT
>AJ305040?GCCATGATGC?CTGGAAATAG?AGCTCAAAAA?GAGTTAATTC?AAGGAAAATT
>AJ305041 GCCATGATGC?CTGGAAACAG?AGCTCAAAAG?GAGTTAATTC?AAGGGAAATT
>AJ305042 GCCATGATGC?CTGGAAATAG?AGCTCAAAAA?GAGTTAATTC?AAGGAAAATT
651 700
NC_001463(gag) AAATGAAGAA?GCAGAAAGGT?GGAGAAGGAA?TAATCCACCA?CCTCCAGCAG
>AJ305040 AAACGAGGAA?GCAGAAAGGT?GGAGAAGGAA?TAATCCACCG?CCTCCACAAG
>AJ305041 AAATGAAGAG?GCAGAAAGGT?GGAGAAGACA?TAATCCACCC?CCTCCGGCGG
>AJ305042 AAATGAGGAA?GCAGAAAGGT?GGAGAAGGAA?TAATCCACCG?CCTCCACAGG
701 750
NC_001463(gag) GAGGAGGATT?AACAGTGGAT?CAAATTATGG?GGGTAGGACA?AACAAATCAA
>AJ305040 GAGGGGGATT?AACAGTGGAT?CAAATTATGG?GGATAGGACA?AACAAATCAA
>AJ305041 GAGGAGGATT?AACAGTGGAT?CAAATTATGG?GGGTAGGACA?AACAAATCAA
>AJ305042 GAGGGGGATT?AACAGTGGAT?CAAATTATGG?GGATAGGACA?AACAAATCAA
751 800
NC_001463(gag) GCAGCAGCAC?AAGCTAACAT?GGATCAGGCA?AGGCAAATAT?GCCTGCAATG
>AJ305040 GCAGCAGCAC?AAGCTAACAT?GGATCAGGCA?AGACACATAT?GCCTGCAATG
>AJ305041 GCAGCAGCAC?AAGCTAACAT?GGATCAGGCA?AGACAAATAT?GCCTGCAATG
>AJ305042 GCAGCAGCAC?AAGCTAACAT?GGATCAGGCA?AGACACATAT?GCCTGCAATG
801 850
NC_001463(gag) GGTAATAAAT?GCATTAAGAG?CAGTAAGACA?TATGGCGCAC?AGGCCAGGGA
>AJ305040 GGTAATAACA?GCATTAAGAG?CAGTAAGACA?TATGGCTCAC?AGACCAGGGA
>AJ305041 GGTAATAACA?GCATTAAGAG?CAGTGAGGTA?TATGACTCAC?AAACCAGGGA
>AJ305042 GGTAATAACA?GCATTAAGAG?CAGTAAGACA?TATGGCTCAC?AGACCAGGGA
851 900
NC_001463(gag) ATCCAATGCT?AGTAAAGCAA?AAAACGAATG?AGCCATATGA?AGATTTTGCA
>AJ305040 ATCCAATGCT?CGTAAAACAA?AAAACAAATG?AGCCATATGA?AGAGTTTGCA
>AJ305041 ATCCAATGCT?AGTAAAACAA?AAAACAAATG?AAGCATATGA?AGAGTTTACA
>AJ305042 ATCCAATGCT?CGTAAAACAA?AAAACAAATG?AGCCATATGA?AGAGTTTGCA
901 950
NC_001463(gag) GCAAGACTGC?TAGAAGCAAT?AGATGCAGAG?CCAGTTACAC?AGCCTATAAA
>AJ305040 GCAAAACTAT?TAGAAGCAAT?AGATGCAGAA?CCAGTAACAC?AGCCCATAAA
>AJ305041 GCGAGACTGC?TAGAAGCAAT?AGATGCAGAG?CCAGTAACAC?AGCCCACAAA
>AJ305042 GCAAAACTAT?TAGAAGCAAT?AGATGCAGAA?CCAGTAACAC?AGCTCATAAA
951 1000
NC_001463(gag) AGATTATCTA?AAGCTAACAC?TATCTTATAC?AAATGCATCA?GCAGATTGTC
>AJ305040 AGACTATCTA?AAGTTAACAT?TATCTTATAC?AAATGCGTCC?TCAGACTGTC
>AJ305041 AGAATATCTA?AAACTAACAT?TATCTTATAC?AAATGCATCC?TCAGACTGTC
>AJ305042 AGACTATCTA?AAGTTAACAT?TATCTTATAC?AAATGCGTCC?TCAGACTGTC
1001 1050
NC_001463(gag) AGAAGCAAAT?GGATAGAACA?CTAGGACAAA?GAGTACAACA?AGCTAGTGTA
>AJ305040 AAAAGCAAAT?GGATAGAGTG?CTGGGACAAA?GAGTGCAACA?AGCTAGTGTA
>AJ305041 AAAAGCAAAT?GGATAGAGTA?CTAGGACAAA?GAGTGCAACA?AGCTAGTGTA
>AJ305042 AAAAGCAAAT?GGATAGAGTG?CTGGGACAAA?GAGTGCAACA?AGCTAGTGTA
1051 1100
NC_001463(gag) GAAGAAAAAA?TGCAAGCATG?TAGAGATGTG?GGATCAGAAG?GGTTCAAAAT
>AJ305040 GACGAGAAAA?TGCAA.....?..........?..........?..........
>AJ305041 GAAGAAAAAA?TGCAA.....?..........?..........?..........
>AJ305042 GACGAGAAGA?TGCAA.....?..........?..........?..........
1101 1150
NC_001463(gag) GCAATTGTTA?GCACAAGCAT?TAAGGCCAGG?AAAAGGAAAA?GGGAATGGAC
>AJ305040 ..........?..........?..........?..........?..........
>AJ305041 ..........?..........?..........?..........?..........
>AJ305042 ..........?..........?..........?..........?..........
1151 1200
NC_001463(gag) AGCCACAAAG?GTGTTACAAC?TGTGGAAAAC?CGGGACATCA?AGCAAGGCAA
>AJ305040 ..........?..........?..........?..........?..........
>AJ305041 ..........?..........?..........?..........?..........
>AJ305042 ..........?..........?..........?..........?..........
1201 1250
NC_001463(gag) TGTAGACAAG?GAATCATATG?TCACAACTGT?GGAAAGAGAG?GACATATGCA
>AJ305040 ..........?..........?..........?..........?..........
>AJ305041 ..........?..........?..........?..........?..........
>AJ305042 ..........?..........?..........?..........?..........
1251 1300
NC_001463(gag) AAAAGAATGC?AGAGGAAAGA?GAGACATAAG?GGGAAAACAG?CAGGGAAACG
>AJ305040 ..........?..........?..........?..........?..........
>AJ305041 ..........?..........?..........?..........?..........
>AJ305042 ..........?..........?..........?..........?..........
1301 1347
NC_001463(gag) GGAGGAGGGG?GATACGTGTG?GTGCCGTCCG?CTCCTCCTAT?GGAATAA
>AJ305040 ..........?..........?..........?..........?..........
>AJ305041 ..........?..........?..........?..........?..........
>AJ305042 ..........?..........?..........?..........?..........
Table 10
PileUp
MSF：728 Type：N Check：9403 ..
Name：NC_001463(gag720bp)?(SEQ?ID?NO：39) Len：728 Check：5765 Weight：0
Name：>AY047362?(SEQ?ID?NO：40) Len：728 Check：3638 Weight：0
//
1 50
NC_001463(gag720bp)?ATGGTGAGTC?TAGATAGAGA?CATGGCGAGG?CAAGTCTCCG?GGGGGAAAAG
>AY047362?..........?..........?..........?..........?..........
51 100
NC_001463(gag720bp)?AGATTATCCT?GAGCTCGAAA?AATGTATCAA?GCATGCATGC?AAGATAAAAG
>AY047362?......TAAA?GATATATTAG?AA.GTATTGG?CCATG.ATGC?CTGGAAATAG
101 150
NC_001463(gag720bp)?TTCGACTCAG?AGGGGAGCAC?TTGACAGAAG?GAAATTGTTT?ATGGTGCCTT
>AY047362?AGC...TCAA?AAAGAGTTAA?TTCA...AGG?GAAATTGAAT?GAAGAAGCAG
151 200
NC_001463(gag720bp)?AAAACATTAG?ATTACATGTT?TGAGGACCAT?AAAGAGGAAC?CTTGGACAAA
>AY047362?AAAGGTGGAG?AAGGAATAAT?CCACCACCTC?AAGCAGG..C?GGAGGATTAA
201 250
NC_001463(gag720bp)?AGTAAAATTT?AGGACAATAT?GGCAGAAGGT?GAAG..AATC?TAACTCCTGA
>AY047362?C..AG..TGG?ATCAAATTAT?GGGGGTAGGA?CAAACAAATC?AAGCAGCGGC
251 300
NC_001463(gag720bp)?GGAGAGTAAC?AAAAAAGACT?TTATGTCTTT?GCAGGCCACA?TTAGCGGGTC
>AY047362?ACAGGCTAAC?ATGGATCAG.?..........?GCAAGACAAA?TATGC..CTG
301 350
NC_001463(gag720bp)?TAATGTGTTG?CCAAATGGGG?ATGAGACCTG?AGACATTGCA?AGATGCAATG
>AY047362?CAATGGGTAA?TAACAGCACT?AAGAGCAGTG?AGACAT....?..A.....TG
351 400
NC_001463(gag720bp)?GCTACAGTAA?TCATGAAAGA?TGGGTTACTG?GAACAAGAGG?AAAAGAAGGA
>AY047362?GCT.CACAAA?CCAGGGA..A?TCCGATGCT.?.....AGT..?AAAGCAA..A
401 450
NC_001463(gag720bp)?AGACAAAAGA?GAAA.AGGAA?GAGAGTGTCT?TCCCAATAGT?AGTGCAAGCA
>AY047362?AAACAAATGA?GTCATATGAA?GATTTTGCCG?...CAAGACT?GCTAGAAGCA
451 500
NC_001463(gag720bp)?GCAGGAGGGA?GAAGCTGGAA?AGCAGTAGAT?TCTGTAATGT?TCCAGCAACT
>AY047362?ATAG.ATGCA?GAACCAGTTA?CAAAGCAAAT?AAAAGAATAT?TT.....AAA
501 550
NC_001463(gag720bp)?GCAAACAGTA?GCAATGCAGC?ATGGCCTCGT?GTCTGAGGAC?TTTGAAAGGC
>AY047362?GTTAACATTA?TCT.TACACA?AATGCATC..?..CTCAG.AC?TGTAAGAAAC
551 600
NC_001463(gag720bp)?AGTTGGCATA?TTATGCTACT?ACCTGGACAA?GTA.AAGACA?TACTAGAAGT
>AY047362?AGATGG.ATA?GAGTACTAGG?ACAGAGAGTG?CAACAAGCTA?GTGTGGAAGA
601 650
NC_001463(gag720bp)?ATTG..GCCA?TGATGCCTGG?AAATAGAGCT?CAAAAGGAGT?TA..ATTCAA
>AY047362?AAAAATGCAA?GCATGCAGAG?ATGT.GGGAT?CAGAAGGATT?CAGAATGC..
651 700
NC_001463(gag720bp)?GGGAAATTAA?ATGAAGAAGC?AGAAAGGTGG?AGAAGGAATA?ATCCACCACC
>AY047362 ..........?..........?..........?..........?..........
701 728
NC_001463(gag720bp) TCCAGCAGGA?GGAGGATTAA?CAGTGGAT
>AY047362?..........?..........?........
PileUp
MSF：1347 Type：N Check：3238 ..
Name：NC_001463(gag)?(SEQ?ID?NO：41) Len：1347 Check：6959 Weight：0
Name：>AY047362?(SEQ?ID?NO：42) Len：1347 Check：6279 Weight：0
//
1 50
NC_001463(gag) ATGGTGAGTC?TAGATAGAGA?CATGGCGAGG?CAAGTCTCCG?GGGGGAAAAG
>AY047362 ..........?..........?..........?..........?..........
51 100
NC_001463(gag) AGATTATCCT?GAGCTCGAAA?AATGTATCAA?GCATGCATGC?AAGATAAAAG
>AY047362 ..........?..........?..........?..........?..........
101 150
NC_001463(gag) TTCGACTCAG?AGGGGAGCAC?TTGACAGAAG?GAAATTGTTT?ATGGTGCCTT
>AY047362 ..........?..........?..........?..........?..........
151 200
NC_001463(gag) AAAACATTAG?ATTACATGTT?TGAGGACCAT?AAAGAGGAAC?CTTGGACAAA
>AY047362 ..........?..........?..........?..........?..........
201 250
NC_001463(gag) AGTAAAATTT?AGGACAATAT?GGCAGAAGGT?GAAGAATCTA?ACTCCTGAGG
>AY047362 ..........?..........?..........?..........?..........
251 300
NC_001463(gag) AGAGTAACAA?AAAAGACTTT?ATGTCTTTGC?AGGCCACATT?AGCGGGTCTA
>AY047362 ..........?..........?..........?..........?..........
301 350
NC_001463(gag) ATGTGTTGCC?AAATGGGGAT?GAGACCTGAG?ACATTGCAAG?ATGCAATGGC
>AY047362 ..........?..........?..........?..........?..........
351 400
NC_001463(gag) TACAGTAATC?ATGAAAGATG?GGTTACTGGA?ACAAGAGGAA?AAGAAGGAAG
>AY047362 ..........?..........?..........?..........?..........
401 450
NC_001463(gag) ACAAAAGAGA?AAAGGAAGAG?AGTGTCTTCC?CAATAGTAGT?GCAAGCAGCA
>AY047362 ..........?..........?..........?..........?..........
451 500
NC_001463(gag) GGAGGGAGAA?GCTGGAAAGC?AGTAGATTCT?GTAATGTTCC?AGCAACTGCA
>AY047362 ..........?..........?..........?..........?..........
501 550
NC_001463(gag) AACAGTAGCA?ATGCAGCATG?GCCTCGTGTC?TGAGGACTTT?GAAAGGCAGT
>AY047362 ..........?..........?..........?..........?..........
551 600
NC_001463(gag) TGGCATATTA?TGCTACTACC?TGGACAAGTA?AAGACATACT?AGAAGTATTG
>AY047362 ..........?..........?........TA?AAGATATATT?AGAAGTATTG
601 650
NC_001463(gag) GCCATGATGC?CTGGAAATAG?AGCTCAAAAG?GAGTTAATTC?AAGGGAAATT
>AY047362 GCCATGATGC?CTGGAAATAG?AGCTCAAAAA?GAGTTAATTC?AAGGGAAATT
651 700
NC_001463(gag) AAATGAAGAA?GCAGAAAGGT?GGAGAAGGAA?TAATCCACCA?CCTCCAGCAG
>AY047362 GAATGAAGAA?GCAGAAAGGT?GGAGAAGGAA?TAATCCACCA?CCTCAAGCAG
701 750
NC_001463(gag) GAGGAGGATT?AACAGTGGAT?CAAATTATGG?GGGTAGGACA?AACAAATCAA
>AY047362 GCGGAGGATT?AACAGTGGAT?CAAATTATGG?GGGTAGGACA?AACAAATCAA
751 800
NC_001463(gag) GCAGCAGCAC?AAGCTAACAT?GGATCAGGCA?AGGCAAATAT?GCCTGCAATG
>AY047362 GCAGCGGCAC?AGGCTAACAT?GGATCAGGCA?AGACAAATAT?GCCTGCAATG
801 850
NC_001463(gag) GGTAATAAAT?GCATTAAGAG?CAGTAAGACA?TATGGCGCAC?AGGCCAGGGA
>AY047362 GGTAATAACA?GCACTAAGAG?CAGTGAGACA?TATGGCTCAC?AAACCAGGGA
851 900
NC_001463(gag) ATCCAATGCT?AGTAAAGCAA?AAAACGAATG?AGCCATATGA?AGATTTTGCA
>AY047362 ATCCGATGCT?AGTAAAGCAA?AAAACAAATG?AGTCATATGA?AGATTTTGCC
901 950
NC_001463(gag) GCAAGACTGC?TAGAAGCAAT?AGATGCAGAG?CCAGTTACAC?AGCCTATAAA
>AY047362 GCAAGACTGC?TAGAAGCAAT?AGATGCAGAA?CCAGTTACAA?AGCAAATAAA
951 1000
NC_001463(gag) AGATTATCTA?AAGCTAACAC?TATCTTATAC?AAATGCATCA?GCAGATTGTC
>AY047362 AGAATATTTA?AAGTTAACAT?TATCTTACAC?AAATGCATCC?TCAGACTGTA
1001 1050
NC_001463(gag) AGAAGCAAAT?GGATAGAACA?CTAGGACAAA?GAGTACAACA?AGCTAGTGTA
>AY047362 AGAAACAGAT?GGATAGAGTA?CTAGGACAGA?GAGTGCAACA?AGCTAGTGTG
1051 1100
NC_001463(gag) GAAGAAAAAA?TGCAAGCATG?TAGAGATGTG?GGATCAGAAG?GGTTCAAAAT
>AY047362 GAAGAAAAAA?TGCAAGCATG?CAGAGATGTG?GGATCAGAAG?GATTCAGAAT
1101 1150
NC_001463(gag) GCAATTGTTA?GCACAAGCAT?TAAGGCCAGG?AAAAGGAAAA?GGGAATGGAC
>AY047362 GC........?..........?..........?..........?..........
1151 1200
NC_001463(gag) AGCCACAAAG?GTGTTACAAC?TGTGGAAAAC?CGGGACATCA?AGCAAGGCAA
>AY047362 ..........?..........?..........?..........?..........
1201 1250
NC_001463(gag) TGTAGACAAG?GAATCATATG?TCACAACTGT?GGAAAGAGAG?GACATATGCA
>AY047362 ..........?..........?..........?..........?..........
1251 1300
NC_001463(gag) AAAAGAATGC?AGAGGAAAGA?GAGACATAAG?GGGAAAACAG?CAGGGAAACG
>AY047362 ..........?..........?..........?..........?..........
1301 1347
NC_001463(gag) GGAGGAGGGG?GATACGTGTG?GTGCCGTCCG?CTCCTCCTAT?GGAATAA
>AY047362 ..........?..........?..........?..........?.......
Table 11
PileUp
MSF：733 Type：N Check：5855 ..
Name：NC_001463(gag720bp)?(SEQ?ID?NO：43)?Len：733?Check：9482 Weight：0
Name：>AY081139?(SEQ?ID?NO：44) Len：733 Check：6373 Weight：0
//
1 50
NC_001463(gag720bp) ATGGTGAGTC?TAGATAGAGA?CATGGCGAGG?CAAGTCTCCG?GGGGGAAAAG
>AY081139 ..........?..........?..........?......TGCC?GTAGACTCTG
51 100
NC_001463(gag720bp) AGATTATCCT?G.AGCTCGAA?AAATGTATCA?AGCATGCATG?CAAGATAAAA
>AY081139 TGATGTTCCA?CCAGCT.GCA?TACAGTAGCA?ATGCCGCATG?GCCTCGTGTC
101 150
NC_001463(gag720bp) GTTCGACTCA?GAGGGGAGCA?CTTGACAGAA?GGAAATTGTT?TATGGTGCCT
>AY081139 TGAGGACTTT?GAAAGG..CA?GTTGGCATAT?TATGCTACTA?CCTGGACAAG
151 200
NC_001463(gag720bp) TAAAACATTA?GATTACATGT?.TTGAGGACC?ATAAAGAGGA?ACCTTGGACA
>AY081139 TAAAGA..TA?TACTGGAAGT?ATTGGCCATG?ATGCCTGGGA?ATAGAGCTCA
201 250
NC_001463(gag720bp) AAAGTAA..A?ATTTAGGACA?ATATGGCAGA?AGGTGAAGAA?TCTAACTCCT
>AY081139 AAAAGAATTA?ATTCAAGGAA?AATTAAATGA?AGAAGCAGAA?..........
251 300
NC_001463(gag720bp) GAGGAGAGTA?ACAAAAAAGA?CTTTATGTCT?TTGCAGGCCA?CATTAGCGGG
>AY081139 .AGGTGGAGA?AGGAATAATC?CACCACCTCA?A.GCAGGCG.?.....GAGGA
301 350
NC_001463(gag720bp) TCTAATGTGT?TGCCAA..AT?GGGGATGAGA?CCTGAGACAT?TGCAAGATGC
>AY081139 TTAACAGTGG?ATCAAATTAT?GGGGGTAGGA?CAAACAAATC?AAGCAGCTGC
351 400
NC_001463(gag720bp) AATGGCTA.C?AGTAATCATG?AAAGATGGGT?TACTGGAACA?AGAGGAAAAG
>AY081139 ACAAGCTAAC?ATGGATCAGG?CAAGACAAAT?A..TGCCTGC?AATGGGTAAT
401 450
NC_001463(gag720bp) AAGGAAGACA?AAAGAGAAAA?GGAAGAGAGT?GTCTTCCCAA?TAGTAGTGCA
>AY081139 ATC..AGCCT?TAAGAGCAGT?GAGACATA.T?GTCT..CATA?AACCAGGG.A
451 500
NC_001463(gag720bp) AGCAGCAGGA?GGGAGAAGCT?GGAAAGCAGT?AGATTCTGTA?ATGTTCCAGC
>AY081139 ATCCGCTGCT?AGTA.AAGCA?AAAAACAAAT?GAGTCATATG?AAGATTTTGC
501 550
NC_001463(gag720bp) AACTGCAAAC?..AGTAGCAA?TGCAGCATGG?CCTCGTGTCT?GAGGACTTTG
>AY081139 AGCTAGACTG?CTAGAAGCAA?TAGATCCAGC?CCCAGTAGCA?CATC.CTATA
551 600
NC_001463(gag720bp)?AAAGGCAGTT?GGCATATTAT?GCTAC....T?ACCTGGACAA?GTAAAGACAT
>AY081139?AAAGATTATT?TAAAGTTAAC?ACTATCTTAT?ACGAATGCAT?CATCAGATTG
601 650
NC_001463(gag720bp) ACTAGAAGTA?TTGGCCATGA?TGCCTGGAAA?TAGAGCTCAA?AAGGAGTTAA
>AY081139 TCAAAAGCAA?ATGGGTAGAA?TGCTAGGATC?GAGAGTCCAT?CA..AGCCAG
651 700
NC_001463(gag720bp) TTCAAGGGAA?ATTAAATGAA?GAAGCAGAAA?GGTGGAGAAG?GAATAATCCA
>AY081139 TGTGGGCCAA?AAAA......?..........?..........?..........
701 733
NC_001463(gag720bp) CCACCTCCAG?CAGGAGGAGG?ATTAACAGTG?GAT
>AY081139..........?..........?..........?...
PileUp
MSF：1347 Type：N Check：2072 ..
Name：NC_001463(gag)?(SEQ?ID?NO：45) Len：1347?Check：6959 Weight：0
Name：>AY081139?(SEQ?ID?NO：46) Len：1347 Check：5113 Weight：0
//
1 50
NC_001463(gag) ATGGTGAGTC?TAGATAGAGA?CATGGCGAGG?CAAGTCTCCG?GGGGGAAAAG
>AY081139 ..........?..........?..........?..........?..........
51 100
NC_001463(gag) AGATTATCCT?GAGCTCGAAA?AATGTATCAA?GCATGCATGC?AAGATAAAAG
>AY081139 ..........?..........?..........?..........?..........
101 150
NC_001463(gag) TTCGACTCAG?AGGGGAGCAC?TTGACAGAAG?GAAATTGTTT?ATGGTGCCTT
>AY081139 ..........?..........?..........?..........?..........
151 200
NC_001463(gag) AAAACATTAG?ATTACATGTT?TGAGGACCAT?AAAGAGGAAC?CTTGGACAAA
>AY081139 ..........?..........?..........?..........?..........
201 250
NC_001463(gag) AGTAAAATTT?AGGACAATAT?GGCAGAAGGT?GAAGAATCTA?ACTCCTGAGG
>AY081139 ..........?..........?..........?..........?..........
251 300
NC_001463(gag) AGAGTAACAA?AAAAGACTTT?ATGTCTTTGC?AGGCCACATT?AGCGGGTCTA
>AY081139 ..........?..........?..........?..........?..........
301 350
NC_001463(gag) ATGTGTTGCC?AAATGGGGAT?GAGACCTGAG?ACATTGCAAG?ATGCAATGGC
>AY081139 ..........?..........?..........?..........?..........
351 400
NC_001463(gag) TACAGTAATC?ATGAAAGATG?GGTTACTGGA?ACAAGAGGAA?AAGAAGGAAG
>AY081139 ..........?..........?..........?..........?..........
401 450
NC_001463(gag) ACAAAAGAGA?AAAGGAAGAG?AGTGTCTTCC?CAATAGTAGT?GCAAGCAGCA
>AY081139 ..........?..........?..........?..........?..........
451 500
NC_001463(gag) GGAGGGAGAA?GCTGGAAAGC?AGTAGATTCT?GTAATGTTCC?AGCAACTGCA
>AY081139 ..........?.......TGC?CGTAGACTCT?GTGATGTTCC?ACCAGCTGCA
501 550
NC_001463(gag) AACAGTAGCA?ATGCAGCATG?GCCTCGTGTC?TGAGGACTTT?GAAAGGCAGT
>AY081139 TACAGTAGCA?ATGCCGCATG?GCCTCGTGTC?TGAGGACTTT?GAAAGGCAGT
551 600
NC_001463(gag) TGGCATATTA?TGCTACTACC?TGGACAAGTA?AAGACATACT?AGAAGTATTG
>AY081139 TGGCATATTA?TGCTACTACC?TGGACAAGTA?AAGATATACT?GGAAGTATTG
601 650
NC_001463(gag) GCCATGATGC?CTGGAAATAG?AGCTCAAAAG?GAGTTAATTC?AAGGGAAATT
>AY081139 GCCATGATGC?CTGGGAATAG?AGCTCAAAAA?GAATTAATTC?AAGGAAAATT
651 700
NC_001463(gag) AAATGAAGAA?GCAGAAAGGT?GGAGAAGGAA?TAATCCACCA?CCTCCAGCAG
>AY081139 AAATGAAGAA?GCAGAAAGGT?GGAGAAGGAA?TAATCCACCA?CCTCAAGCAG
701 750
NC_001463(gag) GAGGAGGATT?AACAGTGGAT?CAAATTATGG?GGGTAGGACA?AACAAATCAA
>AY081139 GCGGAGGATT?AACAGTGGAT?CAAATTATGG?GGGTAGGACA?AACAAATCAA
751 800
NC_001463(gag) GCAGCAGCAC?AAGCTAACAT?GGATCAGGCA?AGGCAAATAT?GCCTGCAATG
>AY081139 GCAGCTGCAC?AAGCTAACAT?GGATCAGGCA?AGACAAATAT?GCCTGCAATG
801 850
NC_001463(gag) GGTAATAAAT?GCATTAAGAG?CAGTAAGACA?TATGGCGCAC?AGGCCAGGGA
>AY081139 GGTAATATCA?GCCTTAAGAG?CAGTGAGACA?TATGTCTCAT?AAACCAGGGA
851 900
NC_001463(gag) ATCCAATGCT?AGTAAAGCAA?AAAACGAATG?AGCCATATGA?AGATTTTGCA
>AY081139 ATCCGCTGCT?AGTAAAGCAA?AAAACAAATG?AGTCATATGA?AGATTTTGCA
901 950
NC_001463(gag) GCAAGACTGC?TAGAAGCAAT?AGATGCAGAG?CCAGTTACAC?AGCCTATAAA
>AY081139 GCTAGACTGC?TAGAAGCAAT?AGATCCAGCC?CCAGTAGCAC?ATCCTATAAA
951 1000
NC_001463(gag) AGATTATCTA?AAGCTAACAC?TATCTTATAC?AAATGCATCA?GCAGATTGTC
>AY081139 AGATTATTTA?AAGTTAACAC?TATCTTATAC?GAATGCATCA?TCAGATTGTC
1001 1050
NC_001463(gag) AGAAGCAAAT?GGATAGAACA?CTAGGACAAA?GAGTACAACA?AGCTAGTGTA
>AY081139 AAAAGCAAAT?GGGTAGAATG?CTAGGATCGA?GAGTCCATCA?AGCCAGTGTG
1051 1100
NC_001463(gag) GAAGAAAAAA?TGCAAGCATG?TAGAGATGTG?GGATCAGAAG?GGTTCAAAAT
>AY081139 GGCCAAAAAA?..........?..........?..........?..........
1101 1150
NC_001463(gag) GCAATTGTTA?GCACAAGCAT?TAAGGCCAGG?AAAAGGAAAA?GGGAATGGAC
>AY081139 ..........?..........?..........?..........?..........
1151 1200
NC_001463(gag) AGCCACAAAG?GTGTTACAAC?TGTGGAAAAC?CGGGACATCA?AGCAAGGCAA
>AY081139 ..........?..........?..........?..........?..........
1201 1250
NC_001463(gag) TGTAGACAAG?GAATCATATG?TCACAACTGT?GGAAAGAGAG?GACATATGCA
>AY081139 ..........?..........?..........?..........?..........
1251 1300
NC_001463(gag) AAAAGAATGC?AGAGGAAAGA?GAGACATAAG?GGGAAAACAG?CAGGGAAACG
>AY081139 ..........?..........?..........?..........?..........
1301 1347
NC_001463(gag) GGAGGAGGGG?GATACGTGTG?GTGCCGTCCG?CTCCTCCTAT?GGAATAA
>AY081139 ..........?..........?..........?..........?..........
Table 12
PileUp
MSF：731 Type：N Check：9546 ..
Name：NC_001463(gag720bp)?(SEQ?ID?NO：47)Len：731?Check：7595 Weight：0
Name：>AY101347?(SEQ?ID?NO：48) Len：731 Check：7962 Weight：0
Name：>AY101348?(SEQ?ID?NO：49) Len：731 Check：3989 Weight：0
//
1 50
NC_001463(gag720bp) ATGGTGAGTC?TAGATAGAGA?CATGGCGAGG?CAAGTCTCCG?GGGGGAAAAG
>AY101347 ..........?..........?..........?..........?..........
>AY101348 ..........?..........?..........?..........?..........
51 100
NC_001463(gag720bp) AGATTATCCT?GAGCTCGAAA?AATGTATCAA?GCATGCATGC?AAGATAAAAG
>AY101347 ..........?..........?.....AGCAG?TAGATTCTGT?AATGTTCCAG
>AY101348 ..........?..........?.....AGCCG?TAGATTCTGT?AATGTTCCAG
101 150
NC_001463(gag720bp) TTCGACT.CA?GAGGGGAGCA?CTTGACAGAA?GGAAATTGTT?TATGGTGCCT
>AY101347 ..CAACTGCA?AACAGTAGCA?AT..GCAGCA?TGGACTCGTG?TATGAAGACT
>AY101348 ..CAGCTGCA?AACAGTAGCA?AT..GCAGCA?TGGCCTCGTG?TCAGAGGACT
151 200
NC_001463(gag720bp) TAAAACATTA?GATTACATGT?TTGAGGACCA?TAAAGAGGAA?CCTTGGACAA
>AY101347 TTGAAAGGCT?GTCGGCATAT?TATGCTACTA?CCTGGAC.AA?GTAAAGATAT
>AY101348 TTGAAAGGCT?TCCAGCATAT?CATGCTACTA?CCTGGGC.AA?GTAAAGATAT
201 250
NC_001463(gag720bp) AAGTAAAATT?TAGGACAATA?TGGCAGAAGG?TGAAGAATCT?AACTCCTGAG
>AY101347 ACTGGAAGTA?TTGGCCATGA?TGCCTG..G.?....GAATAG?AGCTCAAAAA
>AY101348 CTTAGAAGTA?CTGGCCATGA?TGCCTG..G.?....AAATAG?AGCTCAAAAA
251 300
NC_001463(gag720bp) GAGAGTAA..?CAAAAAAGAC?TTTATGTCTT?TGCAGGCCAC?ATTAGCGGGT
>AY101347 GA.ATTAATT?CAAGGAAAAT?TAAATGAAGA?AGCAGAAAGG?TGGAGAAGGA
>AY101348 GA.GTTAATT?CAAGGGAAAT?TAAATGAAGA?AGCAGAGAGG?TGGAGAAGGA
301 350
NC_001463(gag720bp) CTAATGTGTT?GCCAAATGGG?GATGAGACCT?GAGACATTGC?AAGATGCAAT
>AY101347 ATAATCCACC?ACCTCAAGCA?GGCG.GAGGA?TTAACAGTGG?ATCAAATTAT
>AY101348 ATAATCCACC?ACCTCCAGCA?GGAG.GAGGG?TTAACAGTGG?ATCAAATTAT
351 400
NC_001463(gag720bp) GGCTACAGTA?ATCATGAAAG?ATGG.GTTAC?TGGAACAAGA?GGAAAAGAAG
>AY101347 GGGGGTAGGA?CAAACAAATC?AAGCAGCTGC?ACAAGCTAAC?ATGGATCAGG
>AY101348 GGGAGTAGGA?CAAACAAATC?AGGCAGCGGC?ACAAGCAAAC?ATGGATCAGG
401 450
NC_001463(gag720bp) GAAGACAAAA?GAGAAAAGGA?AGAG.AGTGT?CTTCCC.AAT?AGTAGTGCAA
>AY101347 CAAGACAAAT?ATGCCTGCAA?TGGGTAATAT?CAGCCTTAAG?AGCAGTGAGA
>AY101348 CAAGACAAAT?ATGCCTACAA?TGGGTGATAT?CAGCACTAAG?AGCAGTAAGG
451 500
NC_001463(gag720bp) GCAGCAGGAG?GGAGAAGCTG?GAAAGCAGTA?GATTCTGTAA?TGTTCCAGCA
>AY101347 .CATATGTCT?CATAAACCAG?GGAATCCGCT?GCTAGTA.AA?GCAAAAAACA
>AY101348 .CATATGGCT?CACAAGCCAG?GGAATCCAAT?GTTAGTA.AA?GCAAAAAGCA
501 550
NC_001463(gag720bp) ACTG...CAA?ACAGTAGCAA?TGCAGCATGG?CCTCGTGTCT?GAGGACTTTG
>AY101347 AATGAGTCAT?ATGAAGATTT?TGCAGCAAGA?CTGCTAGAAG?CAATAGATGC
>AY101348 AATGAGCCAT?ATGAAGAATT?TGCAGCAAGG?CTGCTGGAAG?CAATAGATGC
551 600
NC_001463(gag720bp) AAAGGCAGTT?GG.CATATTA?TGCTACTACC?TGGACAAGTA?AAGAC.ATAC
>AY101347 AGAGCCAGTA?GCACATCCTA?TAAAAGAATA?CTTA.AAGTT?AACACTATCT
>AY101348 CGAGCCAGTT?AATCAGCCCA?TAAAAGAATA?TCTA.AAACT?AACGTTGTCT
601 650
NC_001463(gag720bp) TAGAAGTATT?GGCCATGATG?CCTGGAAATA?GAGCTCAAAA?GGAGTTAATT
>AY101347 TATACGAATG?CATCATCA.G?ATTGTCAAAA?G....CAAAT?GGATAGAATG
>AY101348 TATACGAATG?CATCCTCA.G?ATTGTCAGAA?G....CAAAT?GGATAGAACA
651 700
NC_001463(gag720bp) CAAGGGAAAT?TAAATGAAGA?AGCAGAAAGG?TGGAGAAGGA?ATAATCCACC
>AY101347 CTGG...AAT?CAAGAGTACA?ACAAGCTAG.?TGTAGAACAA?AAAA......
>AY101348 CTAG...GAC?AAAGAGTCAA?ACAAGCTAG.?TGTAGAACAA?AAAA......
701 731
NC_001463(gag720bp) ACCTCCAGCA?GGAGGAGGAT?TAACAGTGGA?T
>AY101347 ..........?..........?..........?.
>AY101348 ..........?..........?..........?.
PileUp
MSF：1347 Type：N Check：2815 ..
Name：NC_001463(gag)?(SEQ?ID?NO：50) Len：1347?Check：6959 Weight：0
Name：>AY101347?(SEQ?ID?NO：51) Len：1347 Check：969 Weight：0
Name：>AY101348?(SEQ?ID?NO：52) Len：1347 Check：4887 Weight：0
//
1 50
NC_001463(gag)?ATGGTGAGTC?TAGATAGAGA?CATGGCGAGG?CAAGTCTCCG?GGGGGAAAAG
>AY101347?..........?..........?..........?..........?..........
>AY101348?..........?..........?..........?..........?..........
51 100
NC_001463(gag)?AGATTATCCT?GAGCTCGAAA?AATGTATCAA?GCATGCATGC?AAGATAAAAG
>AY101347?..........?..........?..........?..........?..........
>AY101348?..........?..........?..........?..........?..........
101 150
NC_001463(gag)?TTCGACTCAG?AGGGGAGCAC?TTGACAGAAG?GAAATTGTTT?ATGGTGCCTT
>AY101347?..........?..........?..........?..........?..........
>AY101348?..........?..........?..........?..........?..........
151 200
NC_001463(gag)?AAAACATTAG?ATTACATGTT?TGAGGACCAT?AAAGAGGAAC?CTTGGACAAA
>AY101347?..........?..........?..........?..........?..........
>AY101348?..........?..........?..........?..........?..........
201 250
NC_001463(gag)?AGTAAAATTT?AGGACAATAT?GGCAGAAGGT?GAAGAATCTA?ACTCCTGAGG
>AY101347?..........?..........?..........?..........?..........
>AY101348?..........?..........?..........?..........?..........
251 300
NC_001463(gag)?AGAGTAACAA?AAAAGACTTT?ATGTCTTTGC?AGGCCACATT?AGCGGGTCTA
>AY101347?..........?..........?..........?..........?..........
>AY101348?..........?..........?..........?..........?..........
301 350
NC_001463(gag)?ATGTGTTGCC?AAATGGGGAT?GAGACCTGAG?ACATTGCAAG?ATGCAATGGC
>AY101347 ..........?..........?..........?..........?..........
>AY101348 ..........?..........?..........?..........?..........
351 400
NC_001463(gag) TACAGTAATC?ATGAAAGATG?GGTTACTGGA?ACAAGAGGAA?AAGAAGGAAG
>AY101347 ..........?..........?..........?..........?..........
>AY101348 ..........?..........?..........?..........?..........
401 450
NC_001463(gag) ACAAAAGAGA?AAAGGAAGAG?AGTGTCTTCC?CAATAGTAGT?GCAAGCAGCA
>AY101347 ..........?..........?..........?..........?..........
>AY101348 ..........?..........?..........?..........?..........
451 500
NC_001463(gag) GGAGGGAGAA?GCTGGAAAGC?AGTAGATTCT?GTAATGTTCC?AGCAACTGCA
>AY101347 ..........?.......AGC?AGTAGATTCT?GTAATGTTCC?AGCAACTGCA
>AY101348 ..........?.......AGC?CGTAGATTCT?GTAATGTTCC?AGCAGCTGCA
501 550
NC_001463(gag) AACAGTAGCA?ATGCAGCATG?GCCTCGTGTC?TGAGGACTTT?GAAAGGCAGT
>AY101347 AACAGTAGCA?ATGCAGCATG?GACTCGTGTA?TGAAGACTTT?GAAAGGCTGT
>AY101348 AACAGTAGCA?ATGCAGCATG?GCCTCGTGTC?AGAGGACTTT?GAAAGGCTTC
551 600
NC_001463(gag) TGGCATATTA?TGCTACTACC?TGGACAAGTA?AAGACATACT?AGAAGTATTG
>AY101347 CGGCATATTA?TGCTACTACC?TGGACAAGTA?AAGATATACT?GGAAGTATTG
>AY101348 CAGCATATCA?TGCTACTACC?TGGGCAAGTA?AAGATATCTT?AGAAGTACTG
601 650
NC_001463(gag) GCCATGATGC?CTGGAAATAG?AGCTCAAAAG?GAGTTAATTC?AAGGGAAATT
>AY101347 GCCATGATGC?CTGGGAATAG?AGCTCAAAAA?GAATTAATTC?AAGGAAAATT
>AY101348 GCCATGATGC?CTGGAAATAG?AGCTCAAAAA?GAGTTAATTC?AAGGGAAATT
651 700
NC_001463(gag) AAATGAAGAA?GCAGAAAGGT?GGAGAAGGAA?TAATCCACCA?CCTCCAGCAG
>AY101347 AAATGAAGAA?GCAGAAAGGT?GGAGAAGGAA?TAATCCACCA?CCTCAAGCAG
>AY101348 AAATGAAGAA?GCAGAGAGGT?GGAGAAGGAA?TAATCCACCA?CCTCCAGCAG
701 750
NC_001463(gag) GAGGAGGATT?AACAGTGGAT?CAAATTATGG?GGGTAGGACA?AACAAATCAA
>AY101347 GCGGAGGATT?AACAGTGGAT?CAAATTATGG?GGGTAGGACA?AACAAATCAA
>AY101348 GAGGAGGGTT?AACAGTGGAT?CAAATTATGG?GAGTAGGACA?AACAAATCAG
751 800
NC_001463(gag) GCAGCAGCAC?AAGCTAACAT?GGATCAGGCA?AGGCAAATAT?GCCTGCAATG
>AY101347 GCAGCTGCAC?AAGCTAACAT?GGATCAGGCA?AGACAAATAT?GCCTGCAATG
>AY101348 GCAGCGGCAC?AAGCAAACAT?GGATCAGGCA?AGACAAATAT?GCCTACAATG
801 850
NC_001463(gag) GGTAATAAAT?GCATTAAGAG?CAGTAAGACA?TATGGCGCAC?AGGCCAGGGA
>AY101347 GGTAATATCA?GCCTTAAGAG?CAGTGAGACA?TATGTCTCAT?AAACCAGGGA
>AY101348 GGTGATATCA?GCACTAAGAG?CAGTAAGGCA?TATGGCTCAC?AAGCCAGGGA
851 900
NC_001463(gag) ATCCAATGCT?AGTAAAGCAA?AAAACGAATG?AGCCATATGA?AGATTTTGCA
>AY101347 ATCCGCTGCT?AGTAAAGCAA?AAAACAAATG?AGTCATATGA?AGATTTTGCA
>AY101348 ATCCAATGTT?AGTAAAGCAA?AAAGCAAATG?AGCCATATGA?AGAATTTGCA
901 950
NC_001463(gag) GCAAGACTGC?TAGAAGCAAT?AGATGCAGAG?CCAGTTACAC?AGCCTATAAA
>AY101347 GCAAGACTGC?TAGAAGCAAT?AGATGCAGAG?CCAGTAGCAC?ATCCTATAAA
>AY101348 GCAAGGCTGC?TGGAAGCAAT?AGATGCCGAG?CCAGTTAATC?AGCCCATAAA
951 1000
NC_001463(gag)?AGATTATCTA?AAGCTAACAC?TATCTTATAC?AAATGCATCA?GCAGATTGTC
>AY101347?AGAATACTTA?AAGTTAACAC?TATCTTATAC?GAATGCATCA?TCAGATTGTC
>AY101348?AGAATATCTA?AAACTAACGT?TGTCTTATAC?GAATGCATCC?TCAGATTGTC
1001 1050
NC_001463(gag)?AGAAGCAAAT?GGATAGAACA?CTAGGACAAA?GAGTACAACA?AGCTAGTGTA
>AY101347?AAAAGCAAAT?GGATAGAATG?CTGGAATCAA?GAGTACAACA?AGCTAGTGTA
>AY101348?AGAAGCAAAT?GGATAGAACA?CTAGGACAAA?GAGTCAAACA?AGCTAGTGTA
1051 1100
NC_001463(gag)?GAAGAAAAAA?TGCAAGCATG?TAGAGATGTG?GGATCAGAAG?GGTTCAAAAT
>AY101347?GAACAAAAAA?..........?..........?..........?..........
>AY101348?GAACAAAAAA?..........?..........?..........?..........
1101 1150
NC_001463(gag)?GCAATTGTTA?GCACAAGCAT?TAAGGCCAGG?AAAAGGAAAA?GGGAATGGAC
>AY101347?..........?..........?..........?..........?..........
>AY101348?..........?..........?..........?..........?..........
1151 1200
NC_001463(gag)?AGCCACAAAG?GTGTTACAAC?TGTGGAAAAC?CGGGACATCA?AGCAAGGCAA
>AY101347?..........?..........?..........?..........?..........
>AY101348?..........?..........?..........?..........?..........
1201 1250
NC_001463(gag)?TGTAGACAAG?GAATCATATG?TCACAACTGT?GGAAAGAGAG?GACATATGCA
>AY101347?..........?..........?..........?..........?..........
>AY101348?..........?..........?..........?..........?..........
1251 1300
NC_001463(gag)?AAAAGAATGC?AGAGGAAAGA?GAGACATAAG?GGGAAAACAG?CAGGGAAACG
>AY101347?..........?..........?..........?..........?..........
>AY101348?..........?..........?..........?..........?..........
1301 1347
NC_001463(gag)?GGAGGAGGGG?GATACGTGTG?GTGCCGTCCG?CTCCTCCTAT?GGAATAA
>AY101347?..........?..........?..........?..........?.......
>AY101348?..........?..........?..........?..........?.......
Table 13
PileUp
MSF：720 Type：N Check：3690 ..
Name：NC_001463(gag720bp)?(SEQ?ID?NO：53)Len：720?Check：5792?Weight：0
Name：>L78446?(SEQ?ID?NO：54) Len：720 Check：272 Weight：0
Name：>L78447?(SEQ?ID?NO：55) Len：720 Check：1999 Weight：0
Name：>L78450?(SEQ?ID?NO：56) Len：720 Check：9633 Weight：0
Name：>L78451?(SEQ?ID?NO：57) Len：720 Check：5177 Weight：0
Name：>L78453?(SEQ?ID?NO：58) Len：720 Check：817 Weight：0
//
1 50
NC_001463(gag720bp)?ATGGTGAGTC?TAGATAGAGA?CATGGCGAGG?CAAGTCTCCG?GGGGGAAAAG
>L78446?..........?..........?..........?..........?..........
>L78447?..........?..........?..........?..........?..........
>L78450?..........?..........?..........?..........?..........
>L78451?..........?..........?..........?..........?..........
>L78453?..........?..........?..........?..........?..........
51 100
NC_001463(gag720bp)?AGATTATCCT?GAGCTCGAAA?AATGTATCAA?GCATGCATGC?AAGATAAAAG
>L78446?..........?..........?..........?..........?..........
>L78447?..........?..........?..........?..........?..........
>L78450?..........?..........?..........?..........?..........
>L78451?..........?..........?..........?..........?..........
>L78453?..........?..........?..........?..........?..........
101 150
NC_001463(gag720bp)?TTCGACTCAG?AGGGGAGCAC?TTGACAGAAG?GAAATTGTTT?ATGGTGCCTT
>L78446?..........?..........?..........?..........?..........
>L78447?..........?..........?..........?..........?..........
>L78450?..........?..........?..........?..........?..........
>L78451?..........?..........?..........?..........?..........
>L78453?..........?..........?..........?..........?..........
151 200
NC_001463(gag720bp)?AAAACATTAG?ATTACATGTT?TGAGGACCAT?AAAGAGGAAC?CTTGGACAAA
>L78446?..........?..........?..........?..........?..........
>L78447?..........?..........?..........?..........?..........
>L78450?..........?..........?..........?..........?..........
>L78451?..........?..........?..........?..........?..........
>L78453?..........?..........?..........?..........?..........
201 250
NC_001463(gag720bp)?AGTAAAATTT?AGGACAATAT?GGCAGAAGGT?GAAGAATCTA?ACTCCTGAGG
>L78446?..........?..........?..........?..........?..........
>L78447?..........?..........?..........?..........?..........
>L78450?..........?..........?..........?..........?..........
>L78451?..........?..........?..........?..........?..........
>L78453?..........?..........?..........?..........?..........
251 300
NC_001463(gag720bp)?AGAGTAACAA?AAAAGACTTT?ATGTCTTTGC?AGGCCACATT?AGCGGGTCTA
>L78446?..........?..........?..........?..........?..........
>L78447?..........?..........?..........?..........?..........
>L78450?..........?..........?..........?..........?..........
>L78451?..........?..........?..........?..........?..........
>L78453?..........?..........?..........?..........?..........
301 350
NC_001463(gag720bp)?ATGTGTTGCC?AAATGGGGAT?GAGACCTGAG?ACATTGCAAG?ATGCAATGGC
>L78446?..........?..........?..........?..........?..........
>L78447?..........?..........?..........?..........?..........
>L78450?..........?..........?..........?..........?..........
>L78451?..........?..........?..........?..........?..........
>L78453?..........?..........?..........?..........?..........
351 400
NC_001463(gag720bp)?TACAGTAATC?ATGAAAGATG?GGTTACTGGA?ACAAGAGGAA?AAGAAGGAAG
>L78446?..........?..........?..........?..........?..........
>L78447?..........?..........?..........?..........?..........
>L78450?..........?..........?..........?..........?..........
>L78451?..........?..........?..........?..........?..........
>L78453?..........?..........?..........?..........?..........
401 450
NC_001463(gag720bp)?ACAAAAGAGA?AAAGGAAGAG?AGTGTCTTCC?CAATAGTAGT?GCAAGCAGCA
>L78446?..........?..........?..........?..........?..........
>L78447?..........?..........?..........?..........?..........
>L78450?..........?..........?..........?..........?..........
>L78451?..........?..........?..........?..........?..........
>L78453?..........?..........?..........?..........?..........
451 500
NC_001463(gag720bp)?GGAGGGAGAA?GCTGGAAAGC?AGTAGATTCT?GTAATGTTCC?AGCAACTGCA
>L78446?..........?..........?..........?..........?..........
>L78447?..........?..........?..........?..........?..........
>L78450?..........?..........?..........?..........?..........
>L78451?..........?..........?..........?..........?..........
>L78453?..........?..........?..........?..........?..........
501 550
NC_001463(gag720bp)?AACAGTAGCA?ATGCAGCATG?GCCTCGTGTC?TGAGGACTTT?GAAAGGCAGT
>L78446?..........?...CAGCATG?GCCTCGTGTC?CGAGGACTTT?GAAAGGCAGT
>L78447?..........?...CAGCATG?GAATAGTATC?AGAAGAGTTT?GAGAGGCAAC
>L78450?..........?...CAACATG?GGATAGTATC?AGAGGAATTT?GAGAGACAAA
>L78451?..........?...CAGCATG?GACTAGTATC?AGAAGAATTT?GAAAGGCAGC
>L78453?..........?...CAGCATG?GACTTGTGTC?CGAAGATTTT?GAGAGGCAAT
551 600
NC_001463(gag720bp)?TGGCATATTA?TGCTACTACC?TGGACAAGTA?AAGACATACT?AGAAGTATTG
>L78446?TGGCATATTA?TGCTACTACC?TGGACAAGTA?AGGACATATT?AGAAGTATTG
>L78447?TGTCTTATTA?TGCTACCACT?TGGACAAGCA?AGGATATCTT?AGAGGTACTA
>L78450?TGTCTTATTA?TGCTACCACA?TGGACAAGTA?AGGATATTTT?AGAAGTACTA
>L78451?TAGCATACTA?TGCCACAACG?TGGACAAGCA?AAGACATACT?AGAGGTGTTA
>L78453?TGGCATATTA?TGCTACAACC?TGGACTAGTG?AAGATATATT?AGAAGTATTG
601 650
NC_001463(gag720bp)?GCCATGATGC?CTGGAAATAG?AGCTCAAAAG?GAGTTAATTC?AAGGGADAAT
>L78446?GCCATGATGC?CAGGAAATAG?AGCTCAAAAG?GAGCTAATTC?AA........
>L78447?GCCATGATGC?CTGGCAATAG?AGCATTAAAA?GAGCTAATAC?AA........
>L78450?GCAATGATGC?CCGGGAACAG?AGCATTAAAG?GAGCTGATAC?AA........
>L78451?GCCATGATGC?CAGGGAATAG?AGCACAAAAA?GAACTAATAC?AA........
>L78453?GCTATGATGC?CTGGGAATAG?AGCACAGAAA?GAATTAATAC?AA........
651 700
NC_001463(gag720bp)?AAATGAAGAA?GCAGAAAGGT?GGAGAAGGAA?TAATCCACCA?CCTCCAGCAG
>L78446?..........?..........?..........?..........?..........
>L78447?..........?..........?..........?..........?..........
>L78450?..........?..........?..........?..........?..........
>L78451?..........?..........?..........?..........?..........
>L78453?..........?..........?..........?..........?..........
701 720
NC_001463(gag720bp)?GAGGAGGATT?AACAGTGGAT
>L78446?..........?..........
>L78447?..........?..........
>L78450?..........?..........
>L78451?..........?..........
>L78453?..........?..........
PileUp
MSF：1347 Type：N Check：6947 ..
Name：NC_001463(gag)?(SEQ?ID?NO：59)?Len：1347 Check：6959 Weight：0
Name：>L78446?(SEQ?ID?NO：60)?Len：1347 Check：6690 Weight：0
Name：>L78447?(SEQ?ID?NO：61)?Len：1347 Check：8417 Weight：0
Name：>L78450?(SEQ?ID?NO：62)?Len：1347 CheCk：6051 Weight：0
Name：>L78451?(SEQ?ID?NO：63)?Len：1347 Check：1595 Weight：0
Name：>L78453?(SEQ?ID?NO：64)?Len：1347 Check：7235 Weight：0
//
1 50
NC_001463(gag) ATGGTGAGTC?TAGATAGAGA?CATGGCGAGG?CAAGTCTCCG?GGGGGAAAAG
>L78446 ..........?..........?..........?..........?..........
>L78447 ..........?..........?..........?..........?..........
>L78450 ..........?..........?..........?..........?..........
>L78451 ..........?..........?..........?..........?..........
>L78453 ..........?..........?..........?..........?..........
51 100
NC_001463(gag) AGATTATCCT?GAGCTCGAAA?AATGTATCAA?GCATGCATGC?AAGATAAAAG
>L78446 ..........?..........?..........?..........?..........
>L78447 ..........?..........?..........?..........?..........
>L78450 ..........?..........?..........?..........?..........
>L78451 ..........?..........?..........?..........?..........
>L78453 ..........?..........?..........?..........?..........
101 150
NC_001463(gag) TTCGACTCAG?AGGGGAGCAC?TTGACAGAAG?GAAATTGTTT?ATGGTGCCTT
>L78446 ..........?..........?..........?..........?..........
>L78447 ..........?..........?..........?..........?..........
>L78450 ..........?..........?..........?..........?..........
>L78451 ..........?..........?..........?..........?..........
>L78453 ..........?..........?..........?..........?..........
151 200
NC_001463(gag) AAAACATTAG?ATTACATGTT?TGAGGACCAT?AAAGAGGAAC?CTTGGACAAA
>L78446 ..........?..........?..........?..........?..........
>L78447 ..........?..........?..........?..........?..........
>L78450 ..........?..........?..........?..........?..........
>L78451 ..........?..........?..........?..........?..........
>L78453 ..........?..........?..........?..........?..........
201 250
NC_001463(gag) AGTAAAATTT?AGGACAATAT?GGCAGAAGGT?GAAGAATCTA?ACTCCTGAGG
>L78446 ..........?..........?..........?..........?..........
>L78447 ..........?..........?..........?..........?..........
>L78450 ..........?..........?..........?..........?..........
>L78451 ..........?..........?..........?..........?..........
>L78453 ..........?..........?..........?..........?..........
251 300
NC_001463(gag) AGAGTAACAA?AAAAGACTTT?ATGTCTTTGC?AGGCCACATT?AGCGGGTCTA
>L78446 ..........?..........?..........?..........?..........
>L78447 ..........?..........?..........?..........?..........
>L78450 ..........?..........?..........?..........?..........
>L78451 ..........?..........?..........?..........?..........
>L78453 ..........?..........?..........?..........?..........
301 350
NC_001463(gag) ATGTGTTGCC?AAATGGGGAT?GAGACCTGAG?ACATTGCAAG?ATGCAATGGC
>L78446 ..........?..........?..........?..........?..........
>L78447 ..........?..........?..........?..........?..........
>L78450 ..........?..........?..........?..........?..........
>L78451 ..........?..........?..........?..........?..........
>L78453 ..........?..........?..........?..........?..........
351 400
NC_001463(gag) TACAGTAATC?ATGAAAGATG?GGTTACTGGA?ACAAGAGGAA?AAGAAGGAAG
>L78445 ..........?..........?..........?..........?..........
>L78447 ..........?..........?..........?..........?..........
>L78450 ..........?..........?..........?..........?..........
>L78451 ..........?..........?..........?..........?..........
>L78453 ..........?..........?..........?..........?..........
401 450
NC_001463(gag) ACAAAAGAGA?AAAGGAAGAG?AGTGTCTTCC?CAATAGTAGT?GCAAGCAGCA
>L78446 ..........?..........?..........?..........?..........
>L78447 ..........?..........?..........?..........?..........
>L78450 ..........?..........?..........?..........?..........
>L78451 ..........?..........?..........?..........?..........
>L78453 ..........?..........?..........?..........?..........
451 500
NC_001463(gag) GGAGGGAGAA?GCTGGAAAGC?AGTAGATTCT?GTAATGTTCC?AGCAACTGCA
>L78446 ..........?..........?..........?..........?..........
>L78447 ..........?..........?..........?..........?..........
>L78450 ..........?..........?..........?..........?..........
>L78451 ..........?..........?..........?..........?..........
>L78453 ..........?..........?..........?..........?..........
501 550
NC_001463(gag) AACAGTAGCA?ATGCAGCATG?GCCTCGTGTC?TGAGGACTTT?GAAAGGCAGT
>L78446 ..........?...CAGCATG?GCCTCGTGTC?CGAGGACTTT?GAAAGGCAGT
>L78447 ..........?...CAGCATG?GAATAGTATC?AGAAGAGTTT?GAGAGGCAAC
>L78450 ..........?...CAACATG?GGATAGTATC?AGAGGAATTT?GAGAGACAAA
>L78451 ..........?...CAGCATG?GACTAGTATC?AGAAGAATTT?GAAAGGCAGC
>L78453 ..........?...CAGCATG?GACTTGTGTC?CGAAGATTTT?GAGAGGCAAT
551 600
NC_001463(gag) TGGCATATTA?TGCTACTACC?TGGACAAGTA?AAGACATACT?AGAAGTATTG
>L78446 TGGCATATTA?TGCTACTACC?TGGACAAGTA?AGGACATATT?AGAAGTATTG
>L78447 TGTCTTATTA?TGCTACCACT?TGGACAAGCA?AGGATATCTT?AGAGGTACTA
>L78450 TGTCTTATTA?TGCTACCACA?TGGACAAGTA?AGGATATTTT?AGAAGTACTA
>L78451 TAGCATACTA?TGCCACAACG?TGGACAAGCA?AAGACATACT?AGAGGTGTTA
>L78453 TGGCATATTA?TGCTACAACC?TGGACTAGTG?AAGATATATT?AGAAGTATTG
601 650
NC_001463(gag) GCCATGATGC?CTGGAAATAG?AGCTCAAAAG?GAGTTAATTC?AAGGGAAATT
>L78446 GCCATGATGC?CAGGAAATAG?AGCTCAAAAG?GAGCTAATTC?AA........
>L78447 GCCATGATGC?CTGGCAATAG?AGCATTAAAA?GAGCTAATAC?AA........
>L78450 GCAATGATGC?CCGGGAACAG?AGCATTAAAG?GAGCTGATAC?AA........
>L78451 GCCATGATGC?CAGGGAATAG?AGCACAAAAA?GAACTAATAC?AA........
>L78453 GCTATGATGC?CTGGGAATAG?AGCACAGAAA?GAATTAATAC?AA........
651 700
NC_001463(gag) AAATGAAGAA?GCAGAAAGGT?GGAGAAGGAA?TAATCCACCA?CCTCCAGCAG
>L78446 ..........?..........?..........?..........?..........
>L78447 ..........?..........?..........?..........?..........
>L78450 ..........?..........?..........?..........?..........
>L78451 ..........?..........?..........?..........?..........
>L78453 ..........?..........?..........?..........?..........
701 750
NC_001463(gag) GAGGAGGATT?AACAGTGGAT?CAAATTATGG?GGGTAGGACA?AACAAATCAA
>L78446 ..........?..........?..........?..........?..........
>L78447 ..........?..........?..........?..........?..........
>L78450 ..........?..........?..........?..........?..........
>L78451 ..........?..........?..........?..........?..........
>L78453 ..........?..........?..........?..........?..........
751 800
NC_001463(gag)?GCAGCAGCAC?AAGCTAACAT?GGATCAGGCA?AGGCAAATAT?GCCTGCAATG
>L78446?..........?..........?..........?..........?..........
>L78447?..........?..........?..........?..........?..........
>L78450?..........?..........?..........?..........?..........
>L78451?..........?..........?..........?..........?..........
>L78453?..........?..........?..........?..........?..........
801 850
NC_001463(gag)?GGTAATAAAT?GCATTAAGAG?CAGTAAGACA?TATGGCGCAC?AGGCCAGGGA
>L78446?..........?..........?..........?..........?..........
>L78447?..........?..........?..........?..........?..........
>L78450?..........?..........?..........?..........?..........
>L78451?..........?..........?..........?..........?..........
>L78453?..........?..........?..........?..........?..........
851 900
NC_001463(gag)?ATCCAATGCT?AGTAAAGCAA?AAAACGAATG?AGCCATATGA?AGATTTTGCA
>L78446?..........?..........?..........?..........?..........
>L78447?..........?..........?..........?..........?..........
>L78450?..........?..........?..........?..........?..........
>L78451?..........?..........?..........?..........?..........
>L78453?..........?..........?..........?..........?..........
901 950
NC_001463(gag)?GCAAGACTGC?TAGAAGCAAT?AGATGCAGAG?CCAGTTACAC?AGCCTATAAA
>L78446?..........?..........?..........?..........?..........
>L78447?..........?..........?..........?..........?..........
>L78450?..........?..........?..........?..........?..........
>L78451?..........?..........?..........?..........?..........
>L78453?..........?..........?..........?..........?..........
951 1000
NC_001463(gag)?AGATTATCTA?AAGCTAACAC?TATCTTATAC?AAATGCATCA?GCAGATTGTC
>L78446?..........?..........?..........?..........?..........
>L78447?..........?..........?..........?..........?..........
>L78450?..........?..........?..........?..........?..........
>L78451?..........?..........?..........?..........?..........
>L78453?..........?..........?..........?..........?..........
1001 1050
NC_001463(gag)?AGAAGCAAAT?GGATAGAACA?CTAGGACAAA?GAGTACAACA?AGCTAGTGTA
>L78446?..........?..........?..........?..........?..........
>L78447?..........?..........?..........?..........?..........
>L78450?..........?..........?..........?..........?..........
>L78451?..........?..........?..........?..........?..........
>L78453?..........?..........?..........?..........?..........
1051 1100
NC_001463(gag)?GAAGAAAAAA?TGCAAGCATG?TAGAGATGTG?GGATCAGAAG?GGTTCAAAAT
>L78446?..........?..........?..........?..........?..........
>L78447?..........?..........?..........?..........?..........
>L78450?..........?..........?..........?..........?..........
>L78451?..........?..........?..........?..........?..........
>L78453?..........?..........?..........?..........?..........
1101 1150
NC_001463(gag)?GCAATTGTTA?GCACAAGCAT?TAAGGCCAGG?AAAAGGAAAA?GGGAATGGAC
>L78446?..........?..........?..........?..........?..........
>L78447?..........?..........?..........?..........?..........
>L78450?..........?..........?..........?..........?..........
>L78451?..........?..........?..........?..........?..........
>L78453?..........?..........?..........?..........?..........
1151 1200
NC_001463(gag) AGCCACAAAG?GTGTTACAAC?TGTGGAAAAC?CGGGACATCA?AGCAAGGCAA
>L78446 ..........?..........?..........?..........?..........
>L78447 ..........?..........?..........?..........?..........
>L78450 ..........?..........?..........?..........?..........
>L78451 ..........?..........?..........?..........?..........
>L78453 ..........?..........?..........?..........?..........
1201 1250
NC_001463(gag) TGTAGACAAG?GAATCATATG?TCACAACTGT?GGAAAGAGAG?GACATATGCA
>L78446 ..........?..........?..........?..........?..........
>L78447 ..........?..........?..........?..........?..........
>L78450 ..........?..........?..........?..........?..........
>L78451 ..........?..........?..........?..........?..........
>L78453 ..........?..........?..........?..........?..........
1251 1300
NC_001463(gag) AAAAGAATGC?AGAGGAAAGA?GAGACATAAG?GGGAAAACAG?CAGGGAAACG
>L78446 ..........?..........?..........?..........?..........
>L78447 ..........?..........?..........?..........?..........
>L78450 ..........?..........?..........?..........?..........
>L78451 ..........?..........?..........?..........?..........
>L78453 ..........?..........?..........?..........?..........
1301 1347
NC_001463(gag) GGAGGAGGGG?GATACGTGTG?GTGCCGTCCG?CTCCTCCTAT?GGAATAA
>L78446 ..........?..........?..........?..........?..........
>L78447 ..........?..........?..........?..........?..........
>L78450 ..........?..........?..........?..........?..........
>L78451 ..........?..........?..........?..........?..........
>L78453 ..........?..........?..........?..........?..........
Table 14
Gag sequence alignment table
NC_001463 (gag720bp) the vs.AF015181 positive: 41.0% consistence: 41.0%
NC_001463 (gag720bp) >AF015181
NC_001463(gag720bp) 100 41
>AF015181 100
NC_001463 (gag) the vs.AF015181 positive: 40.6% consistence: 40.6%
NC_001463 (gag) >AF015181
NC_001463(gag) 100 41
>AF015181 100
NC_001463 (gag720bp) vs.AF402664～8 positives: 91.1% consistence: 32.2%
NC_001463 (gag720bp) >AF402664 >AF402665 >AF402666 >AF402667 >AF402668
NC_001463(gag720b p) 100 43 44 43 33 43
>AF402664 100 99 96 80 98
>AF402665 100 97 80 99
>AF402666 100 81 96
>AF402667 100 80
>AF402668 100
NC_001463 (gag) vs.AF402664～8 positives: 49.1% consistence: 35.0%
NC_001463 (gag) >AF402664 >AF402665 >AF402666 >AF402667 >AF402668
NC_001463(gag) 100 45 46 44 36 45
>AF402664 100 99 98 89 99
>AF402665 100 98 89 99
>AF402666 100 90 98
>AF402667 100 89
>AF402668 100
NC_001463 (gag720bp) vs.AJ305040～2 positives: 80.5% consistence: 38.1%
NC_001463 (gag720bp) >AJ305040 >AJ305041 >AJ305042
NC_001463(gag720b p) 100 39 42 39
>AJ305040 100 93 99
>AJ305041 100 93
>AJ305042 100
NC_001463 (gag) vs.AJ305040～2 positives: 44.3% consistence: 38.8%
NC_101463 (gag) >AJ305040 >AJ305041 >AJ305042
NC_001463(gag) 100 41 41 40
>AJ305040 100 96 100
>AJ305041 100 96
>AJ305042 100
NC_01463 (ga720bp) the vs.AY047362 positive: 40.2% consistence: 40.2%
NC_001463 (gag720bp) >AY047362
NC_001463(gag720bp) 100 40
>AY047362 100
NC_001463 (gag) the vs.AY047362 positive: 35.7% consistence: 35.7%
NC_001463 (gag) >AY047362
NC_001463(gag) 100 36
>AY047362 100
NC_001463 (gag720bp) the vs.AY081139 positive: 40.0% consistence: 40.0%
NC_001463 (gag720bp) >AY081139
NC_001463(gag720bp) 100 40
>AY081139 100
NC_001463 (gag) the vs.AY081139 positive: 39.8% consistence: 39.8%
NC_001463 (gag) >AY081139
NC_001463(gag) 100 40
>AY081139 100
NC_001463 (gag720bp) vs.AY101347～8 positives: 78.1% consistence: 35.0%
NC_001463 (gag720bp) >AY101347 >AY101348
NC_001463(gag720bp) 100 40 36
>AY101347 100 91
>AY101348 100
NC_001463 (gag) vs.AY101347～8 positives: 43.9% consistence: 37.9%
NC_001463 (gag) >AY101347 >AY101348
NC_001463(gag) 100 41 40
>AY101347 100 95
>AY101348 100
NC_001463 (gag720bp) vs.L78446,7,50,51,53 positives: 17.6% consistence: 11.9%
NC_001463 (gag720bp) >L78446 >L78447 >L78450 >L78451 >L78453
NC_001463(gag720bp) 100 17 14 14 15 15
>L78446 100 96 96 97 97
>L78447 100 98 97 96
>L78450 100 96 96
>L78451 100 97
>L78453 100
NC_001463 (gag) vs.L78446,47,50,51,53 positives: 9.4% consistence: 6.4%
NC_001463 (gag) >L78446 >L78447 >L78450 >L78451 >L78453
NC_001463(gag) 100 9 7 7 8 8
>L78446 100 98 98 98 98
>L78447 100 99 98 98
>L78450 100 98 98
>L78451 100 98
>L78453 100
Table 15
NC_001463 (full genome) vs.AF322109 (full genome) positive: 68.2% consistence: 68.2%
NC_001463 ?AF322109
NC_001463
100 ?68
AF322109 ?100
NC_001463 (gag) vs.AF322109 (gag) positive: 73.1% consistence: 73.1%
NC_001463 (gag) AF322109 (gag)
NC_001463(gag) 100 73
AF322109(gag) 100
NC_001463 (5 ' LTR district) vs.AF322109 (5 ' LTR district) positive: 59.8% consistence: 59.8%
NC_001463 (5′) AF322109 (5′)
NC_001463(5′) 100 60
AF322109(5′) 100
NC_001463 (pol) vs.AF322109 (pol) positive: 74.9% consistence: 74.9%
NC_001463 (pol) AF322109 (Pol)
NC_001463(pol) 100 75
AF322109(pol) 100
NC_001463 (rev) vs.AF322109 (rev) positive: 48.3% consistence: 48.3%
NC_001463 (rev) AF322109 (rev)
NC_001463(rev) 100 48
AF322109(rev) 100
NC_001463 (vif) vs.AF322109 (vif) positive: 66.0% consistence: 66.0%
NC_001463 (vif) AF322109 (vif)
NC_001463(vif) 100 66
AF322109(vif) 100
Sequence table
<110>VECTORCORE?A?CO.，LTD
Jin Lianzhu
Jin Zhongbi
Li Xiujing
＜120〉based on the carrier system of CAEV
<160>79
<170>KopatentIn?1.71
<210>1
<211>9189
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>1
gagttctagg?agagtccctc?ctagtctctc?ctctccgagg?aggtaccgag?acctcaaaat 60
aaaggagtga?ttgccttact?gccgagtgga?gagtgattac?tgagcggccg?gtgtatcggg 120
agtcgtccct?taatctgtgc?aataccagag?cggctctcgc?agctggcgcc?caacgtgggg 180
cccgaggaga?agaaaagaaa?gcggccctga?gaactcggct?tctgaaaaag?aggaagagga 240
caagttgcta?tagcaacaag?agagaagaag?tagagcaaag?gtccagtggc?tcggaaaaag 300
aggaactgaa?acttcgggga?cgcctgaagg?agtaaggtaa?gtgactctgc?tgtacgcggg 360
gcgaggcaga?ggtttccttc?taaattgaaa?gagaagtgtt?gctgcgagag?gtcttggtgg 420
tcgagaatcc?tgtacaaaaa?aaaggaggga?tctcggtcag?gaccaggacc?cctgggagta 480
atacaacagc?aacaccgtaa?gaaaatccgc?catggtgagt?ctagatagag?acatggcgag 540
gcaagtctcc?ggggggaaaa?gagattatcc?tgagctcgaa?aaatgtatca?agcatgcatg 600
caagataaaa?gttcgactca?gaggggagca?cttgacagaa?ggaaattgtt?tatggtgcct 660
taaaacatta?gattacatgt?ttgaggacca?taaagaggaa?ccttggacaa?aagtaaaatt 720
taggacaata?tggcagaagg?tgaagaatct?aactcctgag?gagagtaaca?aaaaagactt 780
tatgtctttg?caggccacat?tagcgggtct?aatgtgttgc?caaatgggga?tgagacctga 840
gacattgcaa?gatgcaatgg?ctacagtaat?catgaaagat?gggttactgg?aacaagagga 900
aaagaaggaa?gacaaaagag?aaaaggaaga?gagtgtcttc?ccaatagtag?tgcaagcagc 960
aggagggaga?agctggaaag?cagtagattc?tgtaatgttc?cagcaactgc?aaacagtagc 1020
aatgcagcat?ggcctcgtgt?ctgaggactt?tgaaaggcag?ttggcatatt?atgctactac 1080
ctggacaagt?aaagacatac?tagaagtatt?ggccatgatg?cctggaaata?gagctcaaaa 1140
ggagttaatt?caagggaaat?taaatgaaga?agcagaaagg?tggagaagga?ataatccacc 1200
acctccagca?ggaggaggat?taacagtgga?tcaaattatg?ggggtaggac?aaacaaatca 1260
agcagcagca?caagctaaca?tggatcaggc?aaggcaaata?tgcctgcaat?gggtaataaa 1320
tgcattaaga?gcagtaagac?atatggcgca?caggccaggg?aatccaatgc?tagtaaagca 1380
aaaaacgaat?gagccatatg?aagattttgc?agcaagactg?ctagaagcaa?tagatgcaga 1440
gccagttaca?cagcctataa?aagattatct?aaagctaaca?ctatcttata?caaatgcatc 1500
agcagattgt?cagaagcaaa?tggatagaac?actaggacaa?agagtacaac?aagctagtgt 1560
agaagaaaaa?atgcaagcat?gtagagatgt?gggatcagaa?gggttcaaaa?tgcaattgtt 1620
agcacaagca?ttaaggccag?gaaaaggaaa?agggaatgga?cagccacaaa?ggtgttacaa 1680
ctgtggaaaa?ccgggacatc?aagcaaggca?atgtagacaa?ggaatcatat?gtcacaactg 1740
tggaaagaga?ggacatatgc?aaaaagaatg?cagaggaaag?agagacataa?ggggaaaaca 1800
gcagggaaac?gggaggaggg?ggatacgtgt?ggtgccgtcc?gctcctccta?tggaataact 1860
tcagcaccac?ctatggttca?ggtccgcata?ggttcccagc?agaggaactt?gttatttgat 1920
accggggcgg?accgaactat?agttagatgg?catgagggct?cgggaaaccc?agccggaagg 1980
ataaaactgc?aaggaatagg?aggaatagta?gaaggagaaa?aatggaataa?tgtagaatta 2040
gaatataaag?gagaaacaag?aaagggaaca?atagtagtgt?taccacaaag?tccagtagaa 2100
gtattaggac?gagataacat?ggcccgattt?ggaataaaga?taataatggc?aaatttagag 2160
gaaaaaagaa?tcccaattac?aaaagtaaaa?ttgaaagagg?gatgtacggg?tccacatgtc 2220
ccacaatggc?cattaacaga?agagaaatta?aaaggtctaa?cagaaatcat?agataaatta 2280
gtggaagaag?gaaaactagg?aaaggcaccc?ccacattgga?catgtaatac?tccaatcttt 2340
tgcataaaaa?agaaatcagg?gaagtggaga?atgttaatag?atttcagaga?attgaacaaa 2400
cagacagaag?atttaacaga?agcgcagtta?ggactcccgc?atccgggagg?actacaaaag 2460
aaaaaacatg?ttacaatatt?ggacatagga?gatgcatatt?ttactatacc?cctatatgaa 2520
ccatatcgag?agtacacatg?ttttactcta?ttaagtccta?ataatctagg?accatgtaaa 2580
agatactatt?ggaaagtgct?gccacaaggt?tggaaattga?gtccatctgt?atatcaattt 2640
actatgcagg?agatcttaga?ggattggata?cagcagcatc?cagaaattca?atttggcata 2700
tatatggatg?atatttacat?aggaagtgat?ttagaaatta?aaaagcatag?agaaatagtg 2760
aaagatttag?ccaattatat?tgcccaatat?ggattcactc?tgccagaaga?gaagagacaa 2820
aagggatatc?cagcaaaatg?gctaggattt?gaactacacc?cgcagacctg?gaaatttcag 2880
aagcatacat?tacctgaatt?aacaaaggga?acaataacat?taaataaatt?acagaaatta 2940
gtaggagaat?tagtatggag?acaatccata?attgggaaaa?gcattcctaa?cattctgaaa 3000
ttaatggaag?gagatagaga?attacaaagt?gaaagaaaaa?ttgaagaagt?acatgtgaaa 3060
gaatgggaag?catgtaggaa?aaaattagaa?gaaatggaag?gaaattatta?taataaagac 3120
aaagatgtct?atggacaatt?ggcttgggga?gacaaagcta?tagaatatat?agtgtatcag 3180
gagaaaggga?aaccattatg?ggtaaatgtg?gttcacaata?taaagaacct?aagcatcccg 3240
caacaggtta?ttaaagcagc?gcaaaaatta?acccaagaag?tcatcattag?gacaggaaaa 3300
ataccatgga?tattgttgcc?agggaaagaa?gaagattgga?gactagaatt?gcaattaggg 3360
aacatcacat?ggatgccaaa?attttggtcc?tgttatcgag?gacatacaag?atggagaaaa 3420
agaaatataa?tagaagaagt?agtagaaggg?cctacatatt?atacagatgg?aggaaaaaag 3480
aataaagtag?gaagtctagg?gttcatagta?tcaacagggg?aaaaatttag?aaagcatgaa 3540
gagggcacaa?accagcaact?agaattaaga?gccatagagg?aagctctaaa?acaagggcct 3600
caaacaatga?atttagtaac?agatagtaga?tatgcatttg?aatttttatt?aagaaattgg 3660
gatgaagaag?taataaagaa?tccaattcaa?gcaagaatta?tggaaattgc?ccacaagaaa 3720
gataggatag?gagtgcattg?ggtgccagga?cataaaggga?ttccccaaaa?tgaagaaata 3780
gacaaatata?tttcggaaat?atttcttgca?aaagaaggag?aaggaattct?cccaaaaaga 3840
gaagaggatg?cagggtatga?tttaatatgc?ccagaagagg?ttaccataga?gccaggacaa 3900
gtgaaatgca?tccccataga?gctaagatta?aatttaaaga?aatcacaatg?ggctatgatt 3960
gctacaaaaa?gcagcatggc?tgccaaagga?gtgttcacac?aaggaggaat?catagactca 4020
ggatatcagg?gacaaataca?ggtaataatg?tataatagca?ataaaatagc?agtagtcata 4080
ccccaaggga?gaaaatttgc?acaattaata?ttaatggata?aaaagcatgg?aaaattggaa 4140
ccctgggggg?aaagcagaaa?aacagaaagg?ggagaaaaag?gatttgggtc?tacaggaatg 4200
tattggatag?aaaatattcc?tctggcagag?gaagaccaca?caaaatggca?tcaagatgcc 4260
cgatcattgc?atctagaatt?tgaaattcca?agaacagcag?cagaagacat?agtaaatcaa 4320
tgtgaaatat?gcaaagaagc?gaggacacct?gcagtaatta?gaggcggaaa?caaaaggggg 4380
gtaaatcatt?ggcaagtgga?ttatacccat?tatgaaaata?tcatactatt?agtatgggta 4440
gaaacaaatt?caggactaat?atatgcagaa?aaagtaaaag?gagaatcagg?gcaagaattc 4500
agaataaaag?tgatgcattg?gtatgcatta?tttggtccag?agtcattgca?gtcagacaat 4560
ggacctgcat?ttgcagcaga?gcccacacag?ctgttaatgc?aatacctagg?agtaaaacac 4620
acaacaggca?taccttggaa?tccacagtct?caggctatag?tagaaagggc?acatcaacta 4680
ttgaaaagca?ctttaaagaa?gttccagcca?caatttgtcg?ctgtagaatc?agccatagca 4740
gcagccctag?tcgccataaa?tataaaaaga?aagggtgggc?tggggacaag?ccctatggat 4800
atttttatat?ataataaaga?acagaaaaga?ataaataata?aatataataa?aaattctcaa 4860
aaaattcaat?tctgttatta?cagaataagg?aaaagaggac?atcaggagag?tggaaaggac 4920
caacccaggt?actgtggaaa?ggggaaggag?ccaattgtgg?taaaggatat?agaaagtgaa 4980
aagtatttag?taatacctta?caaagatgca?aaattcatcc?cgccaccaac?aaaagaaaag 5040
gaataaaaaa?cctggaccag?aattaccctt?agcactatgg?atacatatag?cagaaagcat 5100
taatggggat?agctcatggt?acataacaat?gagactgcaa?cagatgatgt?ggggaaaaag 5160
aggaaataag?ttacaatata?agaatgaaga?cagggaatat?gaaaattggg?aaattacatc 5220
atggggatgg?aaaatgcacc?taaggagagt?gaaacaatgg?atacaagaca?acaggagagg 5280
aagcccatgg?cagtacaaag?taggaggaac?atggaaaagt?ataggagtgt?ggttcctgca 5340
agcaggagat?tacagaaagg?tagacaggca?cttctggtgg?gcatggagga?tactgatatg 5400
ttcctgcagg?aaagaaaagt?ttgatataag?agaatttatg?agaggaagac?atagatggga 5460
tttgtgcaaa?tcctgtgctc?aaggagaagt?agtaaagcat?actagaacaa?aaagtctgga 5520
aagactagta?ctgctacaga?tggtagaaca?gcatgtgttt?caagtattgc?cattgtggag 5580
agccaggaga?agtagtacaa?cagatttccc?atggtgcagg?gacacaacgg?gatacacgca 5640
tgcgtggtct?gtccaggagt?gctggttgat?ggaatatctc?ttagaggatg?agtgaagaac 5700
tgcctcaaag?aagggagaca?catccagaag?aacttgtaag?gaacgtacgg?gaaagagaaa 5760
gggatacatg?gcaatggaca?agcatcagag?tacctgcgga?aatactgcaa?agatggcttg 5820
ctatgcttag?gtcaggcaga?aatagaaaga?aagtgtatag?agaaatgcaa?aaatggatgt 5880
ggatacatcc?caaggcgcct?gtgattaggg?cctgtggatg?cagactatgt?aacccggggt 5940
ggggaacata?atcaagggaa?taataaatgc?aaataaatgt?aactaacaag?tagcaaaagt 6000
gtctgtgtta?gatggatgct?ggggccagat?acatgcgctt?aactgggaag?gaaaactggg 6060
ttgaagtaac?catggacgga?gagaaggaaa?ggaaaagaga?aggtttcact?gcgggacagc 6120
aaggtaagta?tcaaccccag?gtaagtaagc?aaatagggaa?cagaaatact?aacccatgct 6180
ttgcctataa?agggatattc?ctatggagga?tatcactaac?aatgtggata?ttgctaggga 6240
taaatatgtg?tgtcagtgca?gaggattaca?taacactaat?atcagatccc?tatgggttct 6300
cacccataaa?aaatgtgtct?ggggtaccag?tgacttgtgt?aacaaaagaa?ttcgcaaaat 6360
ggggatgtca?accactagga?gcgtaccctg?atccagaaat?agaatacaga?aatgtgagtc 6420
aggaagtagt?gaaagaagta?tatcaagaga?attggccatg?gaatacatat?cattggcctc 6480
tctggcaaat?ggagaatgtt?aggtactggt?taaaagaaaa?tatgcaagaa?aatcaacaga 6540
gaaaaaataa?tacaaaagag?ggtatagagg?aattattagc?aggaactata?aggggaagat 6600
tctgtgtacc?atacccattt?gccttgttaa?aatgcacaaa?gtggtgctgg?tatacagcgg 6660
ccataaacaa?cgagtcagga?aaagcaggaa?aaataaaaat?aaattgcaca?gaagcaagag 6720
cagtctcctg?tacagaggac?atgccattag?cctcaataca?aagagcatat?tgggatgaga 6780
aagacagaga?gagcatggcc?tttatgaata?tcaaagcatg?tgatagcaac?ctaaggtgtc 6840
agaaaagacc?tggagggtgt?atggaaggat?accctatccc?agtaggagca?gaaataatcc 6900
ctgaaagtat?gaaataccta?aggggagcaa?agagtcagta?tgggggaata?aaagataaga 6960
atggagaatt?aaaattacca?ttaacattaa?gagtgtgggt?aaaattagca?aatgtgtcag 7020
aatgggtaaa?tgggacaccc?ccggattggc?aagacagaat?taacggatcc?aaaggaataa 7080
atgggacgct?ctggggagag?cttaacagta?tgcatcacct?aggatttgcc?cttagccaga 7140
acggcaaatg?gtgtaactac?accggggaaa?taaaattagg?gcaagaaaca?ttccaatatc 7200
attacaagcc?aaactggaac?tgtaccggga?attggacgca?atatccggtg?tggcaagtga 7260
ttagaaacct?ggatatggtg?gaacatatga?caggagaatg?tgtgcagaga?ccacaaaggc 7320
acaatataac?agtaggaaat?ggaaccataa?cagggaattg?cagtacaaca?aactgggatg 7380
gatgtaattg?ctcacgatca?ggaaactacc?tatataacag?ctctgaggga?ggattgttat 7440
taattctgtg?cagacaaaac?agcaccctaa?caaggatcct?gggaacaaat?acaaattgga 7500
caactatgtg?gggaatatac?aaaaattgtt?caggatgcga?gaatgcaaca?ttagacaaca 7560
caggagaagg?aaccttagga?ggtgtagcta?ataagaactg?tagcttgcct?cataaaaatg 7620
agagcaacaa?gtggacttgt?gccccaagac?aaagagatgg?aaaaacagat?tcgctataca 7680
tagcaggagg?aaaaaagttt?tggacacgaa?ttaaggccca?attcagctgt?gaaagtaaca 7740
taggacaatt?agatggaatg?ttgcatcagc?aaatactatt?gcaaaaatat?caagtaatta 7800
aggtaagagc?ttatacatat?ggggtgatag?aaatgccaga?aaactatgca?aaaacaagaa 7860
tcataaacag?gaaaaaaaga?gaactcagcc?acaagaggaa?gaagagaggc?gttggcttgg 7920
tcattatgct?agttatcatg?gcaatagtag?ctgccgcagg?ggcttctctg?ggagtcgcaa 7980
acgcgattca?gcagtcttac?actaaggcag?ctgtccagac?ccttgctaat?gcaactgctg 8040
cacagcagga?tgtgttagag?gcaacctatg?ccatggtaca?gcatgtggct?aaaggcgtac 8100
gaatcttgga?agctcgagtg?gctcgagtgg?aagctatcac?agatagaata?atgctatacc 8160
aagaattgga?ttgttggcac?tatcatcaat?actgtataac?ctctacaaaa?acagaagtag 8220
caaaatatat?caattggacg?aggtttaagg?ataattgcac?atggcagcag?tgggagagag 8280
gattacaggg?gtatgataca?aacttaacaa?tactgttaaa?ggaatcagca?gcaatgacac 8340
aactagcaga?agagcaagca?aggaggatac?cagaagtatg?ggaaagttta?aaagacgtct 8400
ttgattggtc?aggatggttc?tcatggctaa?agtatattcc?tattatagta?gtaggattat 8460
taggatgcat?tctgataaga?gctgtgatat?gtgtatgtca?acctcttgtg?cagatataca 8520
gaactctaag?taccccgaca?taccaacggg?tcacagtcat?catggaaaca?agagcagacg 8580
tcgcaggaga?aaatcaggat?tttggcgatg?gcttagagga?atcagacaac?agcgaaacaa 8640
gcgaaagagt?gacagtacag?aaagcttgga?gccgtgcctg?ggagctttgg?cagaactcac 8700
cctggaagga?gccatggaaa?aggggcctgc?tgaggctgct?cgtccttccg?ctgacgatgg 8760
gaatctggat?aaatggatgg?cttggagaac?accacaaaaa?taaaaaaaga?aagggtgact 8820
gtgagacatg?ggctaaagag?gactaataac?aagctaggcc?aaattcctgt?aaatcacttg 8880
gggggttata?agaaaagcaa?gttcactatg?acaaagcaaa?atgtaaaggc?caaattcctg 8940
taaatcactt?ggggggttat?aagaaaagca?agttcactat?gacaaagcaa?aatgtaaccg 9000
caagtgctga?cagatgtaac?agctgacata?tcagctgatg?cttgctcatg?ctgacactgt 9060
agctctgagc?tgtatataag?gagaagcttg?ctgcttgcac?ttcagagttc?taggagagtc 9120
cctcctagtc?tctcctctcc?gaggaggtac?cgagacctca?aaataaagga?gtgattgcct 9180
tactgccga 9189
<210>2
<211>8919
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>2
gtgagtgctc?tgaggagctc?gaaggaaaga?gtcctcagcc?tctcctctcc?gaggagcttc 60
ggctcataat?aaaggagtgc?ttgcttcaac?agaactgagc?tggtcgtggt?tattatcggg 120
gaccgaagtc?ccgtgcaaca?ccggggcggt?tctcgcagct?ggcgcccaac?gtggggctcg 180
agtagcttga?gaagctcgac?tgagatctga?atccaagagc?gacatcagac?agcaagaaat 240
gagagtaatg?agaccgcgag?ctctgctgct?gtaaaaaaga?ggaagtagcg?ggttgccgag 300
gcaactgctc?agaagaacca?ggggaaaggg?cttccagcaa?cctcaaaaga?ggaaccgaga 360
cttcggggac?gcctgaagta?aggtaagtga?ctctgctgta?cgcggggcga?ggcataggag 420
atccttctat?tctaggaaga?gaagcgctgt?tctgggaggt?cttggcgacc?gagaatcttg 480
ttaaataagc?caggatctcg?atcaggacca?agacccctca?ggagagggta?tagacagcgt 540
ggtaagaaat?ccgccgtggt?gagtctagat?agagacatgg?tgaggcaggc?ctccggaagg 600
ggaaaggagt?accccgagct?aaaagaatgt?ctgaaaaagg?catgcaaaat?aaaagtaagg 660
gctggggggg?agcgcctgac?agaaggaaat?tgtctctggt?gtataaaaac?actagagtgt 720
atgtatgagg?attgtaggga?ggaaccttgg?accccagaaa?aatgtaaaca?attatggaaa 780
aagttgaagc?aggtagagcc?tgaggagagt?agcaaagcag?actataactc?gttaaaagca 840
accttggcgg?ggatagtctg?tgtgcaaatg?ggaatgcagc?ccgagacact?gcaggatgcg 900
atagcaacct?taaacatgag?agatgaagta?aaaggaaagg?aaaagccatc?agaagaaaag 960
aagggaatat?atcccatatt?agtgcaggca?ggaggaggaa?gagcatggag?agcggtagag 1020
cctgctacct?ttcagcagct?ccaaacagtg?gcaatgcagc?atggactagt?atcagaagaa 1080
tttgaaaggc?agctagcata?ctatgccacc?acatggacaa?gcaaggatat?cttagaagta 1140
ttagccatga?tgccaggaaa?tagagcgcaa?aaagaactaa?tacaaggaaa?gttaaatgag 1200
gaagcagaga?gatggagaag?gcagaatcca?caacctgcgg?gcgggttaac?cgtggatcag 1260
ataatggggg?taggacaaac?gaatcaggca?gcggcacagg?ctaatatgga?tcaagcaaga 1320
caaatatgcc?tacaatgggt?tataacagca?ataagaggag?ttaggcatat?ggcccataga 1380
ccaggaaatc?ccatgctggt?aagacaaaaa?ccaaatgaga?actatgaaga?gtttgccgca 1440
aggttgttag?aagcagtgga?tgcagaaccc?gttacccaac?ctataaaaga?atatttaaag 1500
gtaactctgt?cttacacaaa?tgcaaattcg?gaatgtcaaa?aacatatgga?cagagtgttg 1560
gggcaaagag?tacagcaggc?ctcaatagaa?gaaaaaatgc?aggcatgcag?ggacatcggg 1620
ggaacagcat?atcagatgca?gttgcttgca?caagccctcc?gtggcggaaa?agaagatggg 1680
aaaaaatctg?tagggaagtg?ttataactgt?ggaaggcccg?gacacagagc?aaaagaatgc 1740
agacaaggca?ttatatgtca?caactgtgga?aaaagagggc?atatacagaa?aaactgcaaa 1800
cagaaaagaa?gaaaggagca?gggaaacatg?aggagggggc?tacgtgtggt?gccgtccgca 1860
ccccctatgg?agtaacgcaa?gcaccactaa?tagttagggt?acaaataggg?aatcaggaga 1920
aacaattatt?atttgacaca?ggggcagata?aaacgatagt?aagaatgcat?gatggaacag 1980
ggattccaaa?cggaagaata?aaattacaag?ggataggagg?aatagtagaa?ggagaaaaat 2040
ggaataaagt?acccatgaca?tataagggag?aaacatcctg?cccaagcttg?gttgtgctaa 2100
gagatagccc?agtagaagta?ttgggaagag?ataacatgga?agcattcggc?gtaaccctaa 2160
taatggcaaa?tttagaagat?aagaaaattc?ccacaatacc?agtagaattg?aaagaaggat 2220
gtaaagggcc?acatgtgccc?cagtggccat?taacagcaga?gaaattacaa?ggactaacag 2280
gaatagtaga?aaaattacta?caggaaggaa?aattggcaga?ggccccagag?ggatggacgt 2340
ggaacacgcc?catcttctgc?ataaaaaaga?agtcaggaaa?atggagaatg?ttaatagatt 2400
ttagggaatt?aaataagcaa?acagcagatt?tagcagaagc?gcagctagga?ctgccacacc 2460
caggagggtt?gcaaaggaaa?aagaatgtaa?caattctgga?cataggagat?gcatatttca 2520
caattccctt?atacgagccc?tatcagaaat?atacatgctt?cacactccta?agtcctaaca 2580
atttgggacc?atgtaaaagg?tattattgga?aagtattacc?ccagggatgg?aaattgagcc 2640
cagctgtata?tcaattcacc?atgcaaaggt?tgttaaaagg?atggatacaa?cagcataaaa 2700
acatacaatt?tggaatatat?atggatgata?tctatattgg?aagtgatcta?acgatagccc 2760
aacataggaa?gataatagaa?gaattagcct?catttataga?acaatttggg?tttacattac 2820
cagaagataa?gagacaagag?ggctatccag?caaaatggct?aggattcgag?ctacatccag 2880
aaaaatggaa?atatcaaaag?cataaattgc?cggaattaca?agagggggta?ataaccctga 2940
acaaattaca?gaagatagta?ggggaattag?tgtggagaca?atccttgata?ggaaagagca 3000
tccccaatat?cataaaatta?atggaaggag?atcgcgcatt?acaaagtgaa?aggaaaatag 3060
aaagaataca?tgtacaagaa?tgggaagcat?gtcaaaagaa?attagatgaa?atggtaggaa 3120
attattacag?agaagaagaa?gatatctatg?gacaaataac?ttggggggat?aaggcaataa 3180
aatacatagt?attccaaagg?aaaggggaac?ccctatgggt?aaatgtagta?catgacataa 3240
aaaatttgag?tctcccacag?caagtgataa?aagcagcaca?gaaattaacc?caggaagtaa 3300
tcataagaac?aggaaaaatc?ccatggctgc?tactaccagg?aagagaagaa?gactggagat 3360
tagaactgca?ggtagggaac?atcacgtgga?tgccatcatt?ttggtcatgt?tatcgaggag 3420
cacccaagtg?gaaaagaagg?aacatagtgg?cagcagtggt?agatggaccg?acatattata 3480
cagatggggg?aaagaaaaac?gcacagggaa?gctttggctt?catctcccca?acaggagaaa 3540
agttcagaag?gcatgaagat?ggaactaatc?aggtattaga?attaagggca?atagaagatc 3600
catgtaaaca?aggacctgaa?agcatgaaca?ttgtaactga?cagcaggtat?gcttatgaat 3660
tcatgctccg?aaactgggat?gaacaggtca?taagaaaccc?cattcaggca?agaatcatgg 3720
cagaagtgca?caagaaaaag?caggtaggaa?tacactgggt?gccagggcat?aaaggaatac 3780
ctcagaatga?agagatagac?cagtacatat?cagaagtatt?cttagcacga?gaaggaacag 3840
ggatatgtga?aaaaaggaag?gaagatgctg?gatatgattt?attatgcccg?catgaggtaa 3900
tacttaaacc?ccaagaagta?aaacggatcc?caatagacct?aaaattaaaa?ttgaaagaaa 3960
agcaatgggc?catgataagt?gggaaaagta?gcgttgcagc?aaaaggaata?tttgtacaag 4020
gaggcataat?agattcaggg?tatcagggac?aagtacaagt?catcctatat?aatagtaata 4080
agatagaggt?caaaatacca?caaggcagga?aatttgccca?attaatatta?atgaacttac 4140
aacatgaaga?attagaagaa?tggggaaagg?aaagaaaaac?agaaagagga?acaaaaggat 4200
ttgggtctac?aggagcattt?tggatagaga?atattcccca?agcagaggaa?gaacattaca 4260
aatggcatca?agatgctaga?tctctgcagc?tagaattcaa?gatacctaga?gcagcagcag 4320
aagacattat?acagcactgt?gaggtatgtc?aagaaggcaa?acccgcagcg?atcacgagag 4380
ggggaaataa?aagaggaata?gatcattggc?aggtagacta?tacacattac?aaagaacaca 4440
taatattagt?atgggtagag?actaattcag?gattaatatt?tgcagagaaa?gtaaaaggag 4500
aatcaggaca?agaatttagg?atgcagacat?tgaaatggta?tgctttgttt?caaccaaaat 4560
cagtgcaatc?agataatggg?acagccttca?cagctgaggc?tacgcagcat?ctaatgaagt 4620
atttagggat?tcagcacact?acgggtattc?cgtggaaccc?ccagtcacaa?agtttagtag 4680
aaagagctca?tcaaacatta?aaacacatgt?tagaaaaatt?agaaccacaa?tttgtggccc 4740
tacagtctgc?catcgcagcc?actctagttg?cgctcaatat?aaaaagaaag?ggtggactag 4800
gggcaagccc?tatggatatt?tacatatata?ataaggagca?acaaagacaa?caagataata 4860
gtaataaatt?aattcagaaa?aaattttgtt?attacaggat?cagaaaaaga?ggccatccag 4920
gagagtggaa?cggcccaact?gaggtactgt?gggaagggga?aggagccata?gtagttaaag 4980
acaaagaaag?tgatagatat?ctagtcatcc?catataaaga?tgcaaaattt?attccgccac 5040
cgtcggaaca?gaagggatag?aagaataggt?ccagaattgc?ctttatcttt?atggacttat 5100
acagcataca?gcataaataa?agatcccgca?tggtatacaa?ccctaagact?gcagcaaatg 5160
atgtggcata?ggaggggaaa?taaattgaca?tatgtcaggg?aaaatgcaca?gtacgaggag 5220
tgggaaatga?cctcgtatga?gtggaggata?agaatgagaa?gggacaaaac?aaaaagtcat 5280
ccaagagggc?atacttcgcc?atggcaatat?cggagacagg?atggatggaa?ggatgtggga 5340
acgtggttcc?tacagccagg?ggactataga?aaggcggatc?agcagttctg?gttcgcttgg 5400
agaatagtgt?cgtgttcatg?taaaaaggaa?ggatttaaca?taagagaatt?tatgctaggt 5460
acccatagat?gggatttgtg?taagtcgtgt?tgccagggtg?aagtagtaaa?gagaacacaa 5520
ccctacacct?tgcaaaggct?cacgtggctt?aaattaacag?aagaccatgt?atttcaagta 5580
atgcccttgt?ggagagctcg?caaagggatt?accatagact?ttccctggtg?cagggacaca 5640
aaaggattcc?tggagccgtg?gacaacgcaa?gagtgttggc?aaatagagta?tcccttggag 5700
gatgagtgag?gaaaccccag?caggaagaga?accgactgca?gaggaaatat?ttgagcaaga 5760
agcagaaagt?tggaagagaa?caagcgtgcg?agtcccaaat?gacatattac?aaagatggct 5820
agcaatgctt?aggcaaagag?gaaatagaaa?gaaagtgctt?agggaaatgc?aaaaatgggc 5880
atggaggaat?cccacggcgc?gggtgattcg?gccgtgtgga?tgtcggctat?gtaaccccgg 5940
ctgggggagt?aattaatcat?aataaagcaa?attgtaacat?gctgtgtcag?gtgtcttgca 6000
ggaatggcgg?agataagaaa?agaagcaaag?gagccactaa?tccagggtaa?gtataaaaaa 6060
caggtaagta?gaataactat?agttatatta?ctaacagtaa?gagcagcact?aggagcagaa 6120
tacataacca?taatatcaga?cccatatggg?ttctctcccg?tgagaaatgt?gtcaggagta 6180
cctgtaactt?gtgtgacaaa?agaatttagt?aagtggggat?gtcagccaat?aggagcctac 6240
ccagacccag?acttagaata?cagaaatata?agtaaagaaa?tattagagga?agtatatcaa 6300
caagactggc?cgtggaatac?ttatcattgg?ccattatggc?aaatggataa?tgtagtacaa 6360
tgggcaaggc?aaaatttaca?ggataaccgc?aaggaaaaaa?gggacctggc?agacctatta 6420
gcaggaaaaa?taaggggaag?attctgtgta?ccctacccat?ttgcgctcct?ggagtgcatg 6480
gaatggtgct?ggtgggttaa?gaacactaat?gcaggggggt?atggagaagc?agacataaga 6540
ataaattgct?caagggcaag?agcagtgagc?tgcacaagtg?aaatgccctt?agcatcccta 6600
cagagggtat?attgggaaaa?ggaggaacga?aaaaacatgg?agaaaatgac?catcaaacct 6660
tgcaataaaa?atttggaatg?caagaacaga?aggggatgcg?cagaagggta?tccagtacct 6720
cccaaggcag?agttattccc?tccagcgttt?caggatttac?agccaaaagg?gtacgcatat 6780
ggggcactta?gagggaacag?caaatttcca?caaagagtgt?cgctaagaac?atgggtgaaa 6840
atagctaacc?tgacaggatg?ggaaaaagga?aagccagcag?aatggtggaa?taccagccaa 6900
caggttcatt?ggtttgatac?cacgccacaa?tatcatttag?gatatgtatt?atcccgagcg 6960
cctgagaaca?ggagttgtaa?tttcacaggg?gaaatacgaa?tagggcaaca?tcagtttgag 7020
tataattaca?ccctgacaaa?gaattgcaca?aaggagaagt?ggaaagagta?ccccatgtgg 7080
catgtctgga?ggcatttaga?tcaaaatgag?cacttatcta?gcatatgttt?caaaagaccg 7140
agaagaaatg?caacacaaat?agggaacagt?acactgcaag?ggcaatgtaa?tagaagtaat 7200
tggacaggat?gccactgcaa?tgagacaggg?ataaacacaa?catggagaat?aaatggcaca 7260
aagggagctt?atctcttaaa?tagcactaat?ggaaacatca?tggtcttgtt?atgctggaac 7320
acaacagtgg?caggggtata?tgagagtcag?ctaaagtgga?atgagagtct?taaagacgga 7380
gactatgggc?tctgttttaa?ttcaacaaac?aggaattgta?ctagaaatgg?agctcggcac 7440
tatgtaaaca?agagagtgat?aaaaaacgac?acagcagatc?ataattgtga?tagcagcata 7500
tcagcaatag?atggaatggt?acatcaacaa?atattactgc?aaaggtatca?agtaattaga 7560
gtaagagctt?acacatacgg?agtgattgat?atgccagaca?attatgagac?cctaccagga 7620
aggagaagga?gagatctcgc?aaaggccagg?aaaaagaggg?gcgtgggcct?ggtcatcatg 7680
ttagctatca?tggccatagt?ggctgctgca?ggagcatctc?tgggagtcgc?gaacgcgatt 7740
cagcagtcct?acaccaggga?cgctgtccag?actcttgcta?acgcgactgc?tgtgcaacag 7800
caggtgttag?aggcgtccta?tgccatgata?cagcatgtgg?ctaagggaat?acgcatcctt 7860
gaagcacgcg?tggcgagaat?ggaagttatg?atggatagaa?tgatgttata?tcaggaagta 7920
gactgctggc?attatcacca?atattgtgta?acctctacaa?gagcagacat?agtgaattac 7980
attaattgga?caaggtttaa?agataattgc?acatggcaag?agtgggaaag?ggagataagt 8040
gcgcatgaag?gaaacatcac?tatattactc?aaagaatcag?caaggataac?acaattagca 8100
caacaaaagg?tacaaagaat?accagatgtg?tggacagcac?taagggagtc?actaggatgg 8160
acacaatggc?tggcttggat?aaaatacctt?cccataatag?tagtagggat?attaggatgc 8220
ataatcataa?gaataatgtt?gtgtgtagta?caaccagttc?ttcagattta?cagaaccttg 8280
actcagacca?ggtatcaaca?agtcaacttg?gtgatggaga?cccgggtgca?actagaagaa 8340
gaagaagaag?aagacggaag?ggatggtgga?gatggctcag?agagatgcag?cgatcccgac 8400
aacaaaggaa?ttatgaacgc?ctggaggaga?gcttgggtga?cttggagaaa?ctcaccttgg 8460
cagaacacat?ggaagaatgt?ggtggtggcg?ccgttggtga?ttccgctgac?aatcagaatt 8520
tggctccttg?gagagaatgg?agagaacccc?taaaagaaaa?ataaaaaggg?tggactgtga 8580
ggactgtgag?gcctaggagc?gagatagaaa?cttataggcc?tctcttcccg?gaaagctaac 8640
tcactgtgag?aggaatagca?agtcacagtg?acactgctaa?ttgtacccgc?aaccctgaga 8700
tcatgcaaac?cacaatcctg?agattatgct?gacatgtgta?acagctgatg?cctcagctga 8760
tgcttgctca?tgctgacaat?gtaactagga?gctctatata?aacagagccc?tagagcttgc 8820
tacttcagag?tgctctgagg?agctcgaagg?aaagagtcct?cagcctctcc?tctccgagga 8880
gcttcggctc?ataataaagg?agtgcttgct?tcaacagaa 8919
<210>3
<211>720
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>3
atggtgagtc?tagatagaga?catggcgagg?caagtctccg?gggggaaaag?agattatcct 60
gagctcgaaa?aatgtatcaa?gcatgcatgc?aagataaaag?ttcgactcag?aggggagcac 120
ttgacagaag?gaaattgttt?atggtgcctt?aaaacattag?attacatgtt?tgaggaccat 180
aaagaggaac?cttggacaaa?agtaaaattt?aggacaatat?ggcagaaggt?gaagaatcta 240
actcctgagg?agagtaacaa?aaaagacttt?atgtctttgc?aggccacatt?agcgggtcta 300
atgtgttgcc?aaatggggat?gagacctgag?acattgcaag?atgcaatggc?tacagtaatc 360
atgaaagatg?ggttactgga?acaagaggaa?aagaaggaag?acaaaagaga?aaaggaagag 420
agtgtcttcc?caatagtagt?gcaagcagca?ggagggagaa?gctggaaagc?agtagattct 480
gtaatgttcc?agcaactgca?aacagtagca?atgcagcatg?gcctcgtgtc?tgaggacttt 540
gaaaggcagt?tggcatatta?tgctactacc?tggacaagta?aagacatact?agaagtattg 600
gccatgatgc?ctggaaatag?agctcaaaag?gagttaattc?aagggaaatt?aaatgaagaa 660
gcagaaaggt?ggagaaggaa?taatccacca?cctccagcag?gaggaggatt?aacagtggat 720
720
<210>4
<211>720
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>4
atggtgaggc?aggcctccgg?aaggggaaag?gagtaccccg?agctaaaaga?atgtctgaaa 60
aaggcatgca?aaataaaagt?aagggctggg?ggggagcgcc?tgacagaagg?aaattgtctc 120
tggtgtataa?aaacactaga?gtgtatgtat?gaggattgta?gggaggaacc?ttggacccca 180
gaaaaatgta?aacaattatg?gaaaaagttg?aagcaggtag?agcctgagga?gagtagcaaa 240
gcagactata?actcgttaaa?agcaaccttg?gcggggatag?tctgtgtgca?aatgggaatg 300
cagcccgaga?cactgcagga?tgcgatagca?accttaaaca?tgagagatga?agtaaaagga 360
aaggaaaagc?catcagaaga?aaagaaggga?atatatccca?tattagtgca?ggcaggagga 420
ggaagagcat?ggagagcggt?agagcctgct?acctttcagc?agctccaaac?agtggcaatg 480
cagcatggac?tagtatcaga?agaatttgaa?aggcagctag?catactatgc?caccacatgg 540
acaagcaagg?atatcttaga?agtattagcc?atgatgccag?gaaatagagc?gcaaaaagaa 600
ctaatacaag?gaaagttaaa?tgaggaagca?gagagatgga?gaaggcagaa?tccacaacct 660
gcgggcgggt?taaccgtgga?tcagataatg?ggggtaggac?aaacgaatca?ggcagcggca 720
720
<210>5
<211>1347
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>5
atggtgagtc?tagatagaga?catggcgagg?caagtctccg?gggggaaaag?agattatcct 60
gagctcgaaa?aatgtatcaa?gcatgcatgc?aagataaaag?ttcgactcag?aggggagcac 120
ttgacagaag?gaaattgttt?atggtgcctt?aaaacattag?attacatgtt?tgaggaccat 180
aaagaggaac?cttggacaaa?agtaaaattt?aggacaatat?ggcagaaggt?gaagaatcta 240
actcctgagg?agagtaacaa?aaaagacttt?atgtctttgc?aggccacatt?agcgggtcta 300
atgtgttgcc?aaatggggat?gagacctgag?acattgcaag?atgcaatggc?tacagtaatc 360
atgaaagatg?ggttactgga?acaagaggaa?aagaaggaag?acaaaagaga?aaaggaagag 420
agtgtcttcc?caatagtagt?gcaagcagca?ggagggagaa?gctggaaagc?agtagattct 480
gtaatgttcc?agcaactgca?aacagtagca?atgcagcatg?gcctcgtgtc?tgaggacttt 540
gaaaggcagt?tggcatatta?tgctactacc?tggacaagta?aagacatact?agaagtattg 600
gccatgatgc?ctggaaatag?agctcaaaag?gagttaattc?aagggaaatt?aaatgaagaa 660
gcagaaaggt?ggagaaggaa?taatccacca?cctccagcag?gaggaggatt?aacagtggat 720
caaattatgg?gggtaggaca?aacaaatcaa?gcagcagcac?aagctaacat?ggatcaggca 780
aggcaaatat?gcctgcaatg?ggtaataaat?gcattaagag?cagtaagaca?tatggcgcac 840
aggccaggga?atccaatgct?agtaaagcaa?aaaacgaatg?agccatatga?agattttgca 900
gcaagactgc?tagaagcaat?agatgcagag?ccagttacac?agcctataaa?agattatcta 960
aagctaacac?tatcttatac?aaatgcatca?gcagattgtc?agaagcaaat?ggatagaaca 1020
ctaggacaaa?gagtacaaca?agctagtgta?gaagaaaaaa?tgcaagcatg?tagagatgtg 1080
ggatcagaag?ggttcaaaat?gcaattgtta?gcacaagcat?taaggccagg?aaaaggaaaa 1140
gggaatggac?agccacaaag?gtgttacaac?tgtggaaaac?cgggacatca?agcaaggcaa 1200
tgtagacaag?gaatcatatg?tcacaactgt?ggaaagagag?gacatatgca?aaaagaatgc 1260
agaggaaaga?gagacataag?gggaaaacag?cagggaaacg?ggaggagggg?gatacgtgtg 1320
gtgccgtccg?ctcctcctat?ggaataa 1347
<210>6
<211>1299
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>6
atggtgaggc?aggcctccgg?aaggggaaag?gagtaccccg?agctaaaaga?atgtctgaaa 60
aaggcatgca?aaataaaagt?aagggctggg?ggggagcgcc?tgacagaagg?aaattgtctc 120
tggtgtataa?aaacactaga?gtgtatgtat?gaggattgta?gggaggaacc?ttggacccca 180
gaaaaatgta?aacaattatg?gaaaaagttg?aagcaggtag?agcctgagga?gagtagcaaa 240
gcagactata?actcgttaaa?agcaaccttg?gcggggatag?tctgtgtgca?aatgggaatg 300
cagcccgaga?cactgcagga?tgcgatagca?accttaaaca?tgagagatga?agtaaaagga 360
aaggaaaagc?catcagaaga?aaagaaggga?atatatccca?tattagtgca?ggcaggagga 420
ggaagagcat?ggagagcggt?agagcctgct?acctttcagc?agctccaaac?agtggcaatg 480
cagcatggac?tagtatcaga?agaatttgaa?aggcagctag?catactatgc?caccacatgg 540
acaagcaagg?atatcttaga?agtattagcc?atgatgccag?gaaatagagc?gcaaaaagaa 600
ctaatacaag?gaaagttaaa?tgaggaagca?gagagatgga?gaaggcagaa?tccacaacct 660
gcgggcgggt?taaccgtgga?tcagataatg?ggggtaggac?aaacgaatca?ggcagcggca 720
caggctaata?tggatcaagc?aagacaaata?tgcctacaat?gggttataac?agcaataaga 780
ggagttaggc?atatggccca?tagaccagga?aatcccatgc?tggtaagaca?aaaaccaaat 840
gagaactatg?aagagtttgc?cgcaaggttg?ttagaagcag?tggatgcaga?acccgttacc 900
caacctataa?aagaatattt?aaaggtaact?ctgtcttaca?caaatgcaaa?ttcggaatgt 960
caaaaacata?tggacagagt?gttggggcaa?agagtacagc?aggcctcaat?agaagaaaaa 1020
atgcaggcat?gcagggacat?cgggggaaca?gcatatcaga?tgcagttgct?tgcacaagcc 1080
ctccgtggcg?gaaaagaaga?tgggaaaaaa?tctgtaggga?agtgttataa?ctgtggaagg 1140
cccggacaca?gagcaaaaga?atgcagacaa?ggcattatat?gtcacaactg?tggaaaaaga 1200
gggcatatac?agaaaaactg?caaacagaaa?agaagaaagg?agcagggaaa?catgaggagg 1260
gggctacgtg?tggtgccgtc?cgcaccccct?atggagtaa 1299
<210>7
<211>511
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>7
gagttctagg?agagtccctc?ctagtctctc?ctctccgagg?aggtaccgag?acctcaaaat 60
aaaggagtga?ttgccttact?gccgagtgga?gagtgattac?tgagcggccg?gtgtatcggg 120
agtcgtccctta?atctgtgc?aataccagag?cggctctcgc?agctggcgcc?caacgtgggg 180
cccgaggaga?agaaaagaaa?gcggccctga?gaactcggct?tctgaaaaag?aggaagagga 240
caagttgcta?tagcaacaag?agagaagaag?tagagcaaag?gtccagtggc?tcggaaaaag 300
aggaactgaa?acttcgggga?cgcctgaagg?agtaaggtaa?gtgactctgc?tgtacgcggg 360
gcgaggcaga?ggtttccttc?taaattgaaa?gagaagtgtt?gctgcgagag?gtcttggtgg 420
tcgagaatcc?tgtacaaaaa?aaaggaggga?tctcggtcag?gaccaggacc?cctgggagta 480
atacaacagc?aacaccgtaa?gaaaatccgc?c 511
<210>8
<211>576
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>8
gtgagtgctc?tgaggagctc?gaaggaaaga?gtcctcagcc?tctcctctcc?gaggagcttc 60
ggctcataat?aaaggagtgc?ttgcttcaac?agaactgagc?tggtcgtggt?tattatcggg 120
gaccgaagtc?ccgtgcaaca?ccggggcggt?tctcgcagct?ggcgcccaac?gtggggctcg 180
agtagcttga?gaagctcgac?tgagatctga?atccaagagc?gacatcagac?agcaagaaat 240
gagagtaatg?agaccgcgag?ctctgctgct?gtaaaaaaga?ggaagtagcg?ggttgccgag 300
gcaactgctc?agaagaacca?ggggaaaggg?cttccagcaa?cctcaaaaga?ggaaccgaga 360
cttcggggac?gcctgaagta?aggtaagtga?ctctgctgta?cgcggggcga?ggcataggag 420
atccttctat?tctaggaaga?gaagcgctgt?tctgggaggt?cttggcgacc?gagaatcttg 480
ttaaataagc?caggatctcg?atcaggacca?agacccctca?ggagagggta?tagacagcgt 540
ggtaagaaat?ccgccgtggt?gagtctagat?agagac 576
<210>9
<211>3318
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>9
atgtcacaac?tgtggaaaga?gaggacatat?gcaaaaagaa?tgcagaggaa?agagagacat 60
aaggggaaaa?cagcagggaa?acgggaggag?ggggatacgt?gtggtgccgt?ccgctcctcc 120
tatggaataa?cttcagcacc?acctatggtt?caggtccgca?taggttccca?gcagaggaac 180
ttgttatttg?ataccggggc?ggaccgaact?atagttagat?ggcatgaggg?ctcgggaaac 240
ccagccggaa?ggataaaact?gcaaggaata?ggaggaatag?tagaaggaga?aaaatggaat 300
aatgtagaat?tagaatataa?aggagaaaca?agaaagggaa?caatagtagt?gttaccacaa 360
agtccagtag?aagtattagg?acgagataac?atggcccgat?ttggaataaa?gataataatg 420
gcaaatttag?aggaaaaaag?aatcccaatt?acaaaagtaa?aattgaaaga?gggatgtacg 480
ggtccacatg?tcccacaatg?gccattaaca?gaagagaaat?taaaaggtct?aacagaaatc 540
atagataaat?tagtggaaga?aggaaaacta?ggaaaggcac?ccccacattg?gacatgtaat 600
actccaatct?tttgcataaa?aaagaaatca?gggaagtgga?gaatgttaat?agatttcaga 660
gaattgaaca?aacagacaga?agatttaaca?gaagcgcagt?taggactccc?gcatccggga 720
ggactacaaa?agaaaaaaca?tgttacaata?ttggacatag?gagatgcata?ttttactata 780
cccctatatg?aaccatatcg?agagtacaca?tgttttactc?tattaagtcc?taataatcta 840
ggaccatgta?aaagatacta?ttggaaagtg?ctgccacaag?gttggaaatt?gagtccatct 900
gtatatcaat?ttactatgca?ggagatctta?gaggattgga?tacagcagca?tccagaaatt 960
caatttggca?tatatatgga?tgatatttac?ataggaagtg?atttagaaat?taaaaagcat 1020
agagaaatag?tgaaagattt?agccaattat?attgcccaat?atggattcac?tctgccagaa 1080
gagaagagac?aaaagggata?tccagcaaaa?tggctaggat?ttgaactaca?cccgcagacc 1140
tggaaatttc?agaagcatac?attacctgaa?ttaacaaagg?gaacaataac?attaaataaa 1200
ttacagaaat?tagtaggaga?attagtatgg?agacaatcca?taattgggaa?aagcattcct 1260
aacattctga?aattaatgga?aggagataga?gaattacaaa?gtgaaagaaa?aattgaagaa 1320
gtacatgtga?aagaatggga?agcatgtagg?aaaaaattag?aagaaatgga?aggaaattat 1380
tataataaag?acaaagatgt?ctatggacaa?ttggcttggg?gagacaaagc?tatagaatat 1440
atagtgtatc?aggagaaagg?gaaaccatta?tgggtaaatg?tggttcacaa?tataaagaac 1500
ctaagcatcc?cgcaacaggt?tattaaagca?gcgcaaaaat?taacccaaga?agtcatcatt 1560
aggacaggaa?aaataccatg?gatattgttg?ccagggaaag?aagaagattg?gagactagaa 1620
ttgcaattag?ggaacatcac?atggatgcca?aaattttggt?cctgttatcg?aggacataca 1680
agatggagaa?aaagaaatat?aatagaagaa?gtagtagaag?ggcctacata?ttatacagat 1740
ggaggaaaaa?agaataaagt?aggaagtcta?gggttcatag?tatcaacagg?ggaaaaattt 1800
agaaagcatg?aagagggcac?aaaccagcaa?ctagaattaa?gagccataga?ggaagctcta 1860
aaacaagggc?ctcaaacaat?gaatttagta?acagatagta?gatatgcatt?tgaattttta 1920
ttaagaaatt?gggatgaaga?agtaataaag?aatccaattc?aagcaagaat?tatggaaatt 1980
gcccacaaga?aagataggat?aggagtgcat?tgggtgccag?gacataaagg?gattccccaa 2040
aatgaagaaa?tagacaaata?tatttcggaa?atatttcttg?caaaagaagg?agaaggaatt 2100
ctcccaaaaa?gagaagagga?tgcagggtat?gatttaatat?gcccagaaga?ggttaccata 2160
gagccaggac?aagtgaaatg?catccccata?gagctaagat?taaatttaaa?gaaatcacaa 2220
tgggctatga?ttgctacaaa?aagcagcatg?gctgccaaag?gagtgttcac?acaaggagga 2280
atcatagact?caggatatca?gggacaaata?caggtaataa?tgtataatag?caataaaata 2340
gcagtagtca?taccccaagg?gagaaaattt?gcacaattaa?tattaatgga?taaaaagcat 2400
ggaaaattgg?aaccctgggg?ggaaagcaga?aaaacagaaa?ggggagaaaa?aggatttggg 2460
tctacaggaa?tgtattggat?agaaaatatt?cctctggcag?aggaagacca?cacaaaatgg 2520
catcaagatg?cccgatcatt?gcatctagaa?tttgaaattc?caagaacagc?agcagaagac 2580
atagtaaatc?aatgtgaaat?atgcaaagaa?gcgaggacac?ctgcagtaat?tagaggcgga 2640
aacaaaaggg?gggtaaatca?ttggcaagtg?gattataccc?attatgaaaa?tatcatacta 2700
ttagtatggg?tagaaacaaa?ttcaggacta?atatatgcag?aaaaagtaaa?aggagaatca 2760
gggcaagaat?tcagaataaa?agtgatgcat?tggtatgcat?tatttggtcc?agagtcattg 2820
cagtcagaca?atggacctgc?atttgcagca?gagcccacac?agctgttaat?gcaataccta 2880
ggagtaaaac?acacaacagg?cataccttgg?aatccacagt?ctcaggctat?agtagaaagg 2940
gcacatcaac?tattgaaaag?cactttaaag?aagttccagc?cacaatttgt?cgctgtagaa 3000
tcagccatag?cagcagccct?agtcgccata?aatataaaaa?gaaagggtgg?gctggggaca 3060
agccctatgg?atatttttat?atataataaa?gaacagaaaa?gaataaataa?taaatataat 3120
aaaaattctc?aaaaaattca?attctgttat?tacagaataa?ggaaaagagg?acatcaggag 3180
agtggaaagg?accaacccag?gtactgtgga?aaggggaagg?agccaattgt?ggtaaaggat 3240
atagaaagtg?aaaagtattt?agtaatacct?tacaaagatg?caaaattcat?cccgccacca 3300
acaaaagaaa?aggaataa 3318
<210>10
<211>3324
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>10
atgcagacaa?ggcattatat?gtcacaactg?tggaaaaaga?gggcatatac?agaaaaactg 60
caaacagaaa?agaagaaagg?agcagggaaa?catgaggagg?gggctacgtg?tggtgccgtc 120
cgcaccccct?atggagtaac?gcaagcacca?ctaatagtta?gggtacaaat?agggaatcag 180
gagaaacaat?tattatttga?cacaggggca?gataaaacga?tagtaagaat?gcatgatgga 240
acagggattc?caaacggaag?aataaaatta?caagggatag?gaggaatagt?agaaggagaa 300
aaatggaata?aagtacccat?gacatataag?ggagaaacat?cctgcccaag?cttggttgtg 360
ctaagagata?gcccagtaga?agtattggga?agagataaca?tggaagcatt?cggcgtaacc 420
ctaataatgg?caaatttaga?agataagaaa?attcccacaa?taccagtaga?attgaaagaa 480
ggatgtaaag?ggccacatgt?gccccagtgg?ccattaacag?cagagaaatt?acaaggacta 540
acaggaatag?tagaaaaatt?actacaggaa?ggaaaattgg?cagaggcccc?agagggatgg 600
acgtggaaca?cgcccatctt?ctgcataaaa?aagaagtcag?gaaaatggag?aatgttaata 660
gattttaggg?aattaaataa?gcaaacagca?gatttagcag?aagcgcagct?aggactgcca 720
cacccaggag?ggttgcaaag?gaaaaagaat?gtaacaattc?tggacatagg?agatgcatat 780
ttcacaattc?ccttatacga?gccctatcag?aaatatacat?gcttcacact?cctaagtcct 840
aacaatttgg?gaccatgtaa?aaggtattat?tggaaagtat?taccccaggg?atggaaattg 900
agcccagctg?tatatcaatt?caccatgcaa?aggttgttaa?aaggatggat?acaacagcat 960
aaaaacatac?aatttggaat?atatatggat?gatatctata?ttggaagtga?tctaacgata 1020
gcccaacata?ggaagataat?agaagaatta?gcctcattta?tagaacaatt?tgggtttaca 1080
ttaccagaag?ataagagaca?agagggctat?ccagcaaaat?ggctaggatt?cgagctacat 1140
ccagaaaaat?ggaaatatca?aaagcataaa?ttgccggaat?tacaagaggg?ggtaataacc 1200
ctgaacaaat?tacagaagat?agtaggggaa?ttagtgtgga?gacaatcctt?gataggaaag 1260
agcatcccca?atatcataaa?attaatggaa?ggagatcgcg?cattacaaag?tgaaaggaaa 1320
atagaaagaa?tacatgtaca?agaatgggaa?gcatgtcaaa?agaaattaga?tgaaatggta 1380
ggaaattatt?acagagaaga?agaagatatc?tatggacaaa?taacttgggg?ggataaggca 1440
ataaaataca?tagtattcca?aaggaaaggg?gaacccctat?gggtaaatgt?agtacatgac 1500
ataaaaaatt?tgagtctccc?acagcaagtg?ataaaagcag?cacagaaatt?aacccaggaa 1560
gtaatcataa?gaacaggaaa?aatcccatgg?ctgctactac?caggaagaga?agaagactgg 1620
agattagaac?tgcaggtagg?gaacatcacg?tggatgccat?cattttggtc?atgttatcga 1680
ggagcaccca?agtggaaaag?aaggaacata?gtggcagcag?tggtagatgg?accgacatat 1740
tatacagatg?ggggaaagaa?aaacgcacag?ggaagctttg?gcttcatctc?cccaacagga 1800
gaaaagttca?gaaggcatga?agatggaact?aatcaggtat?tagaattaag?ggcaatagaa 1860
gatccatgta?aacaaggacc?tgaaagcatg?aacattgtaa?ctgacagcag?gtatgcttat 1920
gaattcatgc?tccgaaactg?ggatgaacag?gtcataagaa?accccattca?ggcaagaatc 1980
atggcagaag?tgcacaagaa?aaagcaggta?ggaatacact?gggtgccagg?gcataaagga 2040
atacctcaga?atgaagagat?agaccagtac?atatcagaag?tattcttagc?acgagaagga 2100
acagggatat?gtgaaaaaag?gaaggaagat?gctggatatg?atttattatg?cccgcatgag 2160
gtaatactta?aaccccaaga?agtaaaacgg?atcccaatag?acctaaaatt?aaaattgaaa 2220
gaaaagcaat?gggccatgat?aagtgggaaa?agtagcgttg?cagcaaaagg?aatatttgta 2280
caaggaggca?taatagattc?agggtatcag?ggacaagtac?aagtcatcct?atataatagt 2340
aataagatag?aggtcaaaat?accacaaggc?aggaaatttg?cccaattaat?attaatgaac 2400
ttacaacatg?aagaattaga?agaatgggga?aaggaaagaa?aaacagaaag?aggaacaaaa 2460
ggatttgggt?ctacaggagc?attttggata?gagaatattc?cccaagcaga?ggaagaacat 2520
tacaaatggc?atcaagatgc?tagatctctg?cagctagaat?tcaagatacc?tagagcagca 2580
gcagaagaca?ttatacagca?ctgtgaggta?tgtcaagaag?gcaaacccgc?agcgatcacg 2640
agagggggaa?ataaaagagg?aatagatcat?tggcaggtag?actatacaca?ttacaaagaa 2700
cacataatat?tagtatgggt?agagactaat?tcaggattaa?tatttgcaga?gaaagtaaaa 2760
ggagaatcag?gacaagaatt?taggatgcag?acattgaaat?ggtatgcttt?gtttcaacca 2820
aaatcagtgc?aatcagataa?tgggacagcc?ttcacagctg?aggctacgca?gcatctaatg 2880
aagtatttag?ggattcagca?cactacgggt?attccgtgga?acccccagtc?acaaagttta 2940
gtagaaagag?ctcatcaaac?attaaaacac?atgttagaaa?aattagaacc?acaatttgtg 3000
gccctacagt?ctgccatcgc?agccactcta?gttgcgctca?atataaaaag?aaagggtgga 3060
ctaggggcaa?gccctatgga?tatttacata?tataataagg?agcaacaaag?acaacaagat 3120
aatagtaata?aattaattca?gaaaaaattt?tgttattaca?ggatcagaaa?aagaggccat 3180
ccaggagagt?ggaacggccc?aactgaggta?ctgtgggaag?gggaaggagc?catagtagtt 3240
aaagacaaag?aaagtgatag?atatctagtc?atcccatata?aagatgcaaa?atttattccg 3300
ccaccgtcgg?aacagaaggg?atag 3324
<210>11
<211>402
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>11
atggatgctg?gggccagata?catgcgctta?actgggaagg?aaaactgggt?tgaagtaacc 60
atggacggag?agaaggaaag?gaaaagagaa?ggtttcactg?cgggacagca?agatatacag 120
aactctaagt?accccgacat?accaacgggt?cacagtcatc?atggaaacaa?gagcagacgt 180
cgcaggagaa?aatcaggattt?tggcgatgg?cttagaggaa?tcagacaaca?gcgaaacaag 240
cgaaagagtg?acagtacaga?aagcttggag?ccgtgcctgg?gagctttggc?agaactcacc 300
ctggaaggag?ccatggaaaa?ggggcctgct?gaggctgctc?gtccttccgc?tgacgatggg 360
aatctggata?aatggatggc?ttggagaaca?ccacaaaaat?aa 402
<210>12
<211>321
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>12
atggcggaga?taagaaaaga?agcaaaggag?ccactaatcc?aggaccaggt?atcaacaagt 60
caacttggtg?atggagaccc?gggtgcaact?agaagaagaa?gaagaagaag?acggaaggga 120
tggtggagat?ggctcagaga?gatgcagcga?tcccgacaac?aaaggaatta?tgaacgcctg 180
gaggagagct?tgggtgactt?ggagaaactc?accttggcag?aacacatgga?agaatgtggt 240
ggtggcgccg?ttggtgattc?cgctgacaat?cagaatttgg?ctccttggag?agaatggaga 300
gaacccctaa?aagaaaaata?a 321
<210>13
<211>690
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>13
atgcaaaatt?catcccgcca?ccaacaaaag?aaaaggaata?aaaaacctgg?accagaatta 60
cccttagcac?tatggataca?tatagcagaa?agcattaatg?gggatagctc?atggtacata 120
acaatgagac?tgcaacagat?gatgtgggga?aaaagaggaa?ataagttaca?atataagaat 180
gaagacaggg?aatatgaaaa?ttgggaaatt?acatcatggg?gatggaaaat?gcacctaagg 240
agagtgaaac?aatggataca?agacaacagg?agaggaagcc?catggcagta?caaagtagga 300
ggaacatgga?aaagtatagg?agtgtggttc?ctgcaagcag?gagattacag?aaaggtagac 360
aggcacttct?ggtgggcatg?gaggatactg?atatgttcct?gcaggaaaga?aaagtttgat 420
ataagagaat?ttatgagagg?aagacataga?tgggatttgt?gcaaatcctg?tgctcaagga 480
gaagtagtaa?agcatactag?aacaaaaagt?ctggaaagac?tagtactgct?acagatggta 540
gaacagcatg?tgtttcaagt?attgccattg?tggagagcca?ggagaagtag?tacaacagat 600
ttcccatggt?gcagggacac?aacgggatac?acgcatgcgt?ggtctgtcca?ggagtgctgg 660
ttgatggaat?atctcttaga?ggatgagtga 690
<210>14
<211>490
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>14
atgcaaaatt?tattccgcca?ccgtcggaac?agaagggata?gaagaatagg?caaaaagtca 60
tccaagaggg?catacttcgc?catggcaata?tcggagacag?gatggatgga?aggatgtggg 120
aacgtggttc?ctacagccag?gggactatag?aaaggcggat?cagcagttct?ggttcgcttg 180
gagaatagtg?tcgtgttcat?gtaaaaagga?aggatttaac?ataagagaat?ttatgctagg 240
tacccataga?tgggatttgt?gtaagtcgtg?ttgccagggt?gaagtagtaa?agagaacaca 300
accctacacc?ttgcaaaggc?tcacgtggct?taaattaaca?gaagaccatg?tatttcaagt 360
aatgcccttg?tggagagctc?gcaaagggat?taccatagac?tttccctggt?gcagggacac 420
aaaaggattc?ctggagccgt?ggacaacgca?agagtgttgg?caaatagagt?atcccttgga 480
ggatgagtga 490
<210>15
<211>720
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>15
atggtgagtc?tagatagaga?catggcgagg?caagtctccg?gggggaaaag?agattatcct 60
gagctcgaaa?aatgtatcaa?gcatgcatgc?aagataaaag?ttcgactcag?aggggagcac 120
ttgacagaag?gaaattgttt?atggtgcctt?aaaacattag?attacatgtt?tgaggaccat 180
aaagaggaac?cttggacaaa?agtaaaattt?aggacaatat?ggcagaaggt?gaagaatcta 240
actcctgagg?agagtaacaa?aaaagacttt?atgtctttgc?aggccacatt?agcgggtcta 300
atgtgttgcc?aaatggggat?gagacctgag?acattgcaag?atgcaatggc?tacagtaatc 360
atgaaagatg?ggttactgga?acaagaggaa?aagaaggaag?acaaaagaga?aaaggaagag 420
agtgtcttcc?caatagtagt?gcaagcagca?ggagggagaa?gctggaaagc?agtagattct 480
gtaatgttcc?agcaactgca?aacagtagca?atgcagcatg?gcctcgtgtc?tgaggacttt 540
gaaaggcagt?tggcatatta?tgctactacc?tggacaagta?aagacatact?agaagtattg 600
gccatgatgc?ctggaaatag?agctcaaaag?gagttaattc?aagggaaatt?aaatgaagaa 660
gcagaaaggt?ggagaaggaa?taatccacca?cctccagcag?gaggaggatt?aacagtggat 720
720
<210>16
<211>591
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>16
gctgtagact?ctgtaatgtt?ccaacaaatg?caaacagtag?caatgcagca?tggcctcgtg 60
tccgaggatt?ttgaaagaca?gttagcatat?tatgctacta?cctggacaag?taaagacata 120
ctagaagtat?tggccatgat?gcctgggaat?agggctcaga?aagaacttat?tcaagggaaa 180
ttgaatgaag?aagcagacag?gtggagaagg?aacaatccac?caggaggatt?aacagtggat 240
caaattatgg?gggtaggaca?aacaaatcaa?gcagcagcac?aagctaacat?ggatcaggca 300
agacaaatat?gcctacaatg?ggtaataaac?gccttaagag?cagtaaggca?tatggctcat 360
aggccaggga?atccaatgct?agtaaagcaa?aaaacaaatg?agccatatga?agaatttgca 420
gcaagactgc?tagaagcaat?agatgcagaa?gcggttacac?agcccataaa?agagtatcta 480
aagctaacat?tatcctatac?aaatgcagcc?tcagattgtc?aaaagcaaat?ggagagagtg 540
ctaggacaaa?gagtacaaca?ggctagtgta?gaaaaaaaaa?tgcaagcatg?t 591
<210>17
<211>1347
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>17
atggtgagtc?tagatagaga?catggcgagg?caagtctccg?gggggaaaag?agattatcct 60
gagctcgaaa?aatgtatcaa?gcatgcatgc?aagataaaag?ttcgactcag?aggggagcac 120
ttgacagaag?gaaattgttt?atggtgcctt?aaaacattag?attacatgtt?tgaggaccat 180
aaagaggaac?cttggacaaa?agtaaaattt?aggacaatat?ggcagaaggt?gaagaatcta 240
actcctgagg?agagtaacaa?aaaagacttt?atgtctttgc?aggccacatt?agcgggtcta 300
atgtgttgcc?aaatggggat?gagacctgag?acattgcaag?atgcaatggc?tacagtaatc 360
atgaaagatg?ggttactgga?acaagaggaa?aagaaggaag?acaaaagaga?aaaggaagag 420
agtgtcttcc?caatagtagt?gcaagcagca?ggagggagaa?gctggaaagc?agtagattct 480
gtaatgttcc?agcaactgca?aacagtagca?atgcagcatg?gcctcgtgtc?tgaggacttt 540
gaaaggcagt?tggcatatta?tgctactacc?tggacaagta?aagacatact?agaagtattg 600
gccatgatgc?ctggaaatag?agctcaaaag?gagttaattc?aagggaaatt?aaatgaagaa 660
gcagaaaggt?ggagaaggaa?taatccacca?cctccagcag?gaggaggatt?aacagtggat 720
caaattatgg?gggtaggaca?aacaaatcaa?gcagcagcac?aagctaacat?ggatcaggca 780
aggcaaatat?gcctgcaatg?ggtaataaat?gcattaagag?cagtaagaca?tatggcgcac 840
aggccaggga?atccaatgct?agtaaagcaa?aaaacgaatg?agccatatga?agattttgca 900
gcaagactgc?tagaagcaat?agatgcagag?ccagttacac?agcctataaa?agattatcta 960
aagctaacac?tatcttatac?aaatgcatca?gcagattgtc?agaagcaaat?ggatagaaca 1020
ctaggacaaa?gagtacaaca?agctagtgta?gaagaaaaaa?tgcaagcatg?tagagatgtg 1080
ggatcagaag?ggttcaaaat?gcaattgtta?gcacaagcat?taaggccagg?aaaaggaaaa 1140
gggaatggac?agccacaaag?gtgttacaac?tgtggaaaac?cgggacatca?agcaaggcaa 1200
tgtagacaag?gaatcatatg?tcacaactgt?ggaaagagag?gacatatgca?aaaagaatgc 1260
agaggaaaga?gagacataag?gggaaaacag?cagggaaacg?ggaggagggg?gatacgtgtg 1320
gtgccgtccg?ctcctcctat?ggaataa 1347
<210>18
<211>591
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>18
gctgtagact?ctgtaatgtt?ccaacaaatg?caaacagtag?caatgcagca?tggcctcgtg 60
tccgaggatt?ttgaaagaca?gttagcatat?tatgctacta?cctggacaag?taaagacata 120
ctagaagtat?tggccatgat?gcctgggaat?agggctcaga?aagaacttat?tcaagggaaa 180
ttgaatgaag?aagcagacag?gtggagaagg?aacaatccac?caggaggatt?aacagtggat 240
caaattatgg?gggtaggaca?aacaaatcaa?gcagcagcac?aagctaacat?ggatcaggca 300
agacaaatat?gcctacaatg?ggtaataaac?gccttaagag?cagtaaggca?tatggctcat 360
aggccaggga?atccaatgct?agtaaagcaa?aaaacaaatg?agccatatga?agaatttgca 420
gcaagactgc?tagaagcaat?agatgcagaa?gcggttacac?agcccataaa?agagtatcta 480
aagctaacat?tatcctatac?aaatgcagcc?tcagattgtc?aaaagcaaat?ggagagagtg 540
ctaggacaaa?gagtacaaca?ggctagtgta?gaaaaaaaaa?tgcaagcatg?t 591
<210>19
<211>720
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>19
atggtgagtc?tagatagaga?catggcgagg?caagtctccg?gggggaaaag?agattatcct 60
gagctcgaaa?aatgtatcaa?gcatgcatgc?aagataaaag?ttcgactcag?aggggagcac 120
ttgacagaag?gaaattgttt?atggtgcctt?aaaacattag?attacatgtt?tgaggaccat 180
aaagaggaac?cttggacaaa?agtaaaattt?aggacaatat?ggcagaaggt?gaagaatcta 240
actcctgagg?agagtaacaa?aaaagacttt?atgtctttgc?aggccacatt?agcgggtcta 300
atgtgttgcc?aaatggggat?gagacctgag?acattgcaag?atgcaatggc?tacagtaatc 360
atgaaagatg?ggttactgga?acaagaggaa?aagaaggaag?acaaaagaga?aaaggaagag 420
agtgtcttcc?caatagtagt?gcaagcagca?ggagggagaa?gctggaaagc?agtagattct 480
gtaatgttcc?agcaactgca?aacagtagca?atgcagcatg?gcctcgtgtc?tgaggacttt 540
gaaaggcagt?tggcatatta?tgctactacc?tggacaagta?aagacatact?agaagtattg 600
gccatgatgc?ctggaaatag?agctcaaaag?gagttaattc?aagggaaatt?aaatgaagaa 660
gcagaaaggt?ggagaaggaa?taatccacca?cctccagcag?gaggaggatt?aacagtggat 720
720
<210>20
<211>662
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>20
tcaagcagca?ggagggagaa?gctggaaagc?agtagactca?gtgatgttcc?agcaactgca 60
aaatgtagca?atgcagcatg?gcctcgtgtc?cgaggatttt?gaaaggcagt?tagtatatta 120
tgctactacc?tggacaagta?aagatatatt?agaagtattg?gccatgatgc?ctggaaatag 180
agctcaaaaa?gagttaattc?aagggaaatt?gaatgaggaa?gcagaaaggt?ggagaagaaa 240
taatccacca?cctcaagcag?gcggaggatt?aacagtggat?caaattatgg?gggtaggaca 300
aacaaatcaa?gcagcggcac?aggctaacat?ggatcaggca?agacaaatat?gtctgcaatg 360
ggtaataaca?gcactaagag?cagtgagaca?tatggctcac?aaaccaggga?atccaatgct 420
agtaaagcaa?aaaacaaatg?agtcatatga?agattttgcc?gcaagactgc?tagaagcaat 480
agatgcagaa?ccagttacac?agcaaataaa?agaatattta?aagttaacat?tatcttacac 540
aaatgcatcc?tcagactgtc?agaaacagat?ggatagagta?ctaggacaga?gagtgcaaca 600
agctagtgtg?gaagaaaaaa?tgcaagcatg?cagagatgtg?ggatcagaag?gattcagaat 660
gc 662
<210>21
<211>662
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>21
gcaagcagca?ggagggagaa?gctggaaagc?agtagactca?gtgatgttcc?agcaactgca 60
aaatgtagca?atgcagcatg?gcctcgtgtc?cgaggatttt?gaaaggcagt?tagcatatta 120
tgctactacc?tggacaagta?aagatatatt?agaagtattg?gccatgatgc?ctggaaatag 180
agctcaaaaa?gagttaattc?aagggaaatt?gaatgaggaa?gcagaaaggt?ggagaaggaa 240
taatccacca?cctcaagcag?gcggaggatt?aacagtggat?caaattatgg?gggtaggaca 300
aacaaatcaa?gcagcggcac?aggctaacat?ggatcaggca?agacaaatat?gcctgcaatg 360
ggtaataaca?gcactaagag?cagtgagaca?tatggctcac?aaaccaggga?atccaatgct 420
agtaaagcaa?aagacaaatg?agtcatatga?agattttgcc?gcaagactgc?tagaagcaat 480
agatgcagaa?ccagttacac?agcaaataaa?agaatattta?aagttaacat?tatcttacac 540
aaatgcatcc?tcagactgtc?aaaaacaaat?ggatagaata?ctaggacaga?gagtgcaaca 600
agctagtgtg?gaagaaaaaa?tgcaagcatg?cagagatgtg?ggatcagaag?ggttcagaat 660
gc 662
<210>22
<211>651
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>22
gcaagcagca?ggagggagaa?gctggaaagc?agtagactca?gtgatgttcc?agcaactgca 60
aaatgtagca?atgcagcatg?gcctcgtgtc?cgaggatttt?gaaaggcagt?tggcatatta 120
tgctactacc?tggacaagta?aagatatatt?agaagtattg?gccatgatgc?ctggaaacag 180
agctcaaaaa?gagttaattc?aggggaaatt?gaataaggaa?gcagaaaggt?ggagaaggaa 240
taatccacca?cctcaagcac?aaggaggatt?aacagtggat?caaattatgg?gggtaggaca 300
aacaaatcag?gcagcggcac?aggctaacat?ggatcaggca?agacaaatat?gcctgcaatg 360
ggtaataaca?gcactaagag?cagtgagaca?tatggctcac?aaaccaggga?atccaatgct 420
agtaaagcaa?aagacaaatg?agtcatatga?agattttgcc?gcaagactgc?tagaagcaat 480
agatgcagaa?ccagttacac?agcaaataaa?agaatattta?aagttaacat?tatcttacac 540
aaatgcatcc?tcagactgtc?agaaacaaat?ggatagagta?ctaggacaga?gagtgcaaca 600
agctagtgtg?gaagaaaaaa?tgcaagcatg?cagagatgtg?ggatcagaag?g 651
<210>23
<211>520
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>23
gcaagcagca?ggagggagaa?gctggaaagc?agtagactca?gtgatgttcc?agcaactgca 60
aaatgtagca?atgcagcatg?gcctcgtgtc?cgaggatttt?gaaaggcagt?tagcatatta 120
tgctactacc?tggacaagta?aagatatatt?agaagtattg?gccatgatgc?ctggaaatag 180
agctcaaaaa?gagttaattc?aagggaaatt?gaatgaggaa?gcagaaaggt?ggagaaggaa 240
taatccacca?cctcaagcag?gcggaggatt?aacagtggat?caaattatgg?gggtaggaca 300
aacaaatcaa?gcagcggcac?aggctaacat?ggatcaggca?agacaaatat?gcctgcaatg 360
ggtaataaca?gcactaagag?cagtgagaca?tatggctcac?aaaccaggga?atccaatgct 420
agtaaagcaa?aagacaaatg?agtcatatga?agattttgcc?gcaagactgc?tagaagcaat 480
agatgcagaa?ccagttacac?agcaaataaa?gaatatttaa 520
<210>24
<211>663
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>24
gcaagcagca?ggagggagaa?gctggaaagc?agtagactca?gtgatgttcc?agcaactgca 60
aaatgtagca?atgcagcatg?gcctcgtgtc?cgaggatttt?gaaaggcagt?tagcatatta 120
tgctactacc?tggacaagta?aagatatatt?agaagtattg?gccatgatgc?ctggaaatag 180
agctcaaaaa?gagttaattc?aagggaaatt?gaatgaggaa?gcagaaaggt?ggagaaggaa 240
taatccacca?cctcaagcag?gcggaggatt?aacagtggat?caaattatgg?gggtaggaca 300
aacaaatcaa?gcagcggcac?aggctaacat?ggatcaggca?agacaaatat?gcctgcaatg 360
ggtaataaca?gcgactaaga?gcagtgagac?atatggctca?caaaccaggg?aatccaatgc 420
tagtaaagca?aaagacaaat?gagtcatatg?aaaaattttc?agcaagactc?ctagaagcaa 480
tagatgcaga?accagttaca?cagcctataa?aagaatattt?aaagttaaca?ttatcttaca 540
caaatgcatc?ctcagactgt?caaaaacaaa?tggatagagt?actaggacag?agagtgcaac 600
aagctagtgt?ggaagaaaaa?atgcaagcat?gcagagatgt?gggatcagaa?ggattcagaa 660
tgc 663
<210>25
<211>1347
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>25
atggtgagtc?tagatagaga?catggcgagg?caagtctccg?gggggaaaag?agattatcct 60
gagctcgaaa?aatgtatcaa?gcatgcatgc?aagataaaag?ttcgactcag?aggggagcac 120
ttgacagaag?gaaattgttt?atggtgcctt?aaaacattag?attacatgtt?tgaggaccat 180
aaagaggaac?cttggacaaa?agtaaaattt?aggacaatat?ggcagaaggt?gaagaatcta 240
actcctgagg?agagtaacaa?aaaagacttt?atgtctttgc?aggccacatt?agcgggtcta 300
atgtgttgcc?aaatggggat?gagacctgag?acattgcaag?atgcaatggc?tacagtaatc 360
atgaaagatg?ggttactgga?acaagaggaa?aagaaggaag?acaaaagaga?aaaggaagag 420
agtgtcttcc?caatagtagt?gcaagcagca?ggagggagaa?gctggaaagc?agtagattct 480
gtaatgttcc?agcaactgca?aacagtagca?atgcagcatg?gcctcgtgtc?tgaggacttt 540
gaaaggcagt?tggcatatta?tgctactacc?tggacaagta?aagacatact?agaagtattg 600
gccatgatgc?ctggaaatag?agctcaaaag?gagttaattc?aagggaaatt?aaatgaagaa 660
gcagaaaggt?ggagaaggaa?taatccacca?cctccagcag?gaggaggatt?aacagtggat 720
caaattatgg?gggtaggaca?aacaaatcaa?gcagcagcac?aagctaacat?ggatcaggca 780
aggcaaatat?gcctgcaatg?ggtaataaat?gcattaagag?cagtaagaca?tatggcgcac 840
aggccaggga?atccaatgct?agtaaagcaa?aaaacgaatg?agccatatga?agattttgca 900
gcaagactgc?tagaagcaat?agatgcagag?ccagttacac?agcctataaa?agattatcta 960
aagctaacac?tatcttatac?aaatgcatca?gcagattgtc?agaagcaaat?ggatagaaca 1020
ctaggacaaa?gagtacaaca?agctagtgta?gaagaaaaaa?tgcaagcatg?tagagatgtg 1080
ggatcagaag?ggttcaaaat?gcaattgtta?gcacaagcat?taaggccagg?aaaaggaaaa 1140
gggaatggac?agccacaaag?gtgttacaac?tgtggaaaac?cgggacatca?agcaaggcaa 1200
tgtagacaag?gaatcatatg?tcacaactgt?ggaaagagag?gacatatgca?aaaagaatgc 1260
agaggaaaga?gagacataag?gggaaaacag?cagggaaacg?ggaggagggg?gatacgtgtg 1320
gtgccgtccg?ctcctcctat?ggaataa 1347
<210>26
<211>662
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>26
tcaagcagca?ggagggagaa?gctggaaagc?agtagactca?gtgatgttcc?agcaactgca 60
aaatgtagca?atgcagcatg?gcctcgtgtc?cgaggatttt?gaaaggcagt?tagtatatta 120
tgctactacc?tggacaagta?aagatatatt?agaagtattg?gccatgatgc?ctggaaatag 180
agctcaaaaa?gagttaattc?aagggaaatt?gaatgaggaa?gcagaaaggt?ggagaagaaa 240
taatccacca?cctcaagcag?gcggaggatt?aacagtggat?caaattatgg?gggtaggaca 300
aacaaatcaa?gcagcggcac?aggctaacat?ggatcaggca?agacaaatat?gtctgcaatg 360
ggtaataaca?gcactaagag?cagtgagaca?tatggctcac?aaaccaggga?atccaatgct 420
agtaaagcaa?aaaacaaatg?agtcatatga?agattttgcc?gcaagactgc?tagaagcaat 480
agatgcagaa?ccagttacac?agcaaataaa?agaatattta?aagttaacat?tatcttacac 540
aaatgcatcc?tcagactgtc?agaaacagat?ggatagagta?ctaggacaga?gagtgcaaca 600
agctagtgtg?gaagaaaaaa?tgcaagcatg?cagagatgtg?ggatcagaag?gattcagaat 660
gc 662
<210>27
<211>662
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>27
gcaagcagca?ggagggagaa?gctggaaagc?agtagactca?gtgatgttcc?agcaactgca 60
aaatgtagca?atgcagcatg?gcctcgtgtc?cgaggatttt?gaaaggcagt?tagcatatta 120
tgctactacc?tggacaagta?aagatatatt?agaagtattg?gccatgatgc?ctggaaatag 180
agctcaaaaa?gagttaattc?aagggaaatt?gaatgaggaa?gcagaaaggt?ggagaaggaa 240
taatccacca?cctcaagcag?gcggaggatt?aacagtggat?caaattatgg?gggtaggaca 300
aacaaatcaa?gcagcggcac?aggctaacat?ggatcaggca?agacaaatat?gcctgcaatg 360
ggtaataaca?gcactaagag?cagtgagaca?tatggctcac?aaaccaggga?atccaatgct 420
agtaaagcaa?aagacaaatg?agtcatatga?agattttgcc?gcaagactgc?tagaagcaat 480
agatgcagaa?ccagttacac?agcaaataaa?agaatattta?aagttaacat?tatcttacac 540
aaatgcatcc?tcagactgtc?aaaaacaaat?ggatagaata?ctaggacaga?gagtgcaaca 600
agctagtgtg?gaagaaaaaa?tgcaagcatg?cagagatgtg?ggatcagaag?ggttcagaat 660
gc 662
<210>28
<211>651
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>28
gcaagcagca?ggagggagaa?gctggaaagc?agtagactca?gtgatgttcc?agcaactgca 60
aaatgtagca?atgcagcatg?gcctcgtgtc?cgaggatttt?gaaaggcagt?tggcatatta 120
tgctactacc?tggacaagta?aagatatatt?agaagtattg?gccatgatgc?ctggaaacag 180
agctcaaaaa?gagttaattc?aggggaaatt?gaataaggaa?gcagaaaggt?ggagaaggaa 240
taatccacca?cctcaagcac?aaggaggatt?aacagtggat?caaattatgg?gggtaggaca 300
aacaaatcag?gcagcggcac?aggctaacat?ggatcaggca?agacaaatat?gcctgcaatg 360
ggtaataaca?gcactaagag?cagtgagaca?tatggctcac?aaaccaggga?atccaatgct 420
agtaaagcaa?aagacaaatg?agtcatatga?agattttgcc?gcaagactgc?tagaagcaat 480
agatgcagaa?ccagttacac?agcaaataaa?agaatattta?aagttaacat?tatcttacac 540
aaatgcatcc?tcagactgtc?agaaacaaat?ggatagagta?ctaggacaga?gagtgcaaca 600
agctagtgtg?gaagaaaaaa?tgcaagcatg?cagagatgtg?ggatcagaag?g 651
<210>29
<211>520
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>29
gcaagcagca?ggagggagaa?gctggaaagc?agtagactca?gtgatgttcc?agcaactgca 60
aaatgtagca?atgcagcatg?gcctcgtgtc?cgaggatttt?gaaaggcagt?tagcatatta 120
tgctactacc?tggacaagta?aagatatatt?agaagtattg?gccatgatgc?ctggaaatag 180
agctcaaaaa?gagttaattc?aagggaaatt?gaatgaggaa?gcagaaaggt?ggagaaggaa 240
taatccacca?cctcaagcag?gcggaggatt?aacagtggat?caaattatgg?gggtaggaca 300
aacaaatcaa?gcagcggcac?aggctaacat?ggatcaggca?agacaaatat?gcctgcaatg 360
ggtaataaca?gcactaagag?cagtgagaca?tatggctcac?aaaccaggga?atccaatgct 420
agtaaagcaa?aagacaaatg?agtcatatga?agattttgcc?gcaagactgc?tagaagcaat 480
agatgcagaa?ccagttacac?agcaaataaa?gaatatttaa 520
<210>30
<211>662
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>30
gcaagcagca?ggagggagaa?gctggaaagc?agtagactca?gtgatgttcc?agcaactgca 60
aaatgtagca?atgcagcatg?gcctcgtgtc?cgaggatttt?gaaaggcagt?tagcatatta 120
tgctactacc?tggacaagta?aagatatatt?agaagtattg?gccatgatgc?ctggaaatag 180
agctcaaaaa?gagttaattc?aagggaaatt?gaatgaggaa?gcagaaaggt?ggagaaggaa 240
taatccacca?cctcaagcag?gcggaggatt?aacagtggat?caaattatgg?gggtaggaca 300
aacaaatcaa?gcagcggcac?aggctaacat?ggatcaggca?agacaaatat?gcctgcaatg 360
ggtaataaca?gcactaagag?cagtgagaca?tatggctcac?aaaccaggga?atccaatgct 420
agtaaagcaa?aagacaaatg?agtcatatga?aaaattttca?gcaagactcc?tagaagcaat 480
agatgcagaa?ccagttacac?agcctataaa?agaatattta?aagttaacat?tatcttacac 540
aaatgcatcc?tcagactgtc?aaaaacaaat?ggatagagta?ctaggacaga?gagtgcaaca 600
agctagtgtg?gaagaaaaaa?tgcaagcatg?cagagatgtg?ggatcagaag?gattcagaat 660
gc 662
<210>31
<211>720
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>31
atggtgagtc?tagatagaga?catggcgagg?caagtctccg?gggggaaaag?agattatcct 60
gagctcgaaa?aatgtatcaa?gcatgcatgc?aagataaaag?ttcgactcag?aggggagcac 120
ttgacagaag?gaaattgttt?atggtgcctt?aaaacattag?attacatgtt?tgaggaccat 180
aaagaggaac?cttggacaaa?agtaaaattt?aggacaatat?ggcagaaggt?gaagaatcta 240
actcctgagg?agagtaacaa?aaaagacttt?atgtctttgc?aggccacatt?agcgggtcta 300
atgtgttgcc?aaatggggat?gagacctgag?acattgcaag?atgcaatggc?tacagtaatc 360
atgaaagatg?ggttactgga?acaagaggaa?aagaaggaag?acaaaagaga?aaaggaagag 420
agtgtcttcc?caatagtagt?gcaagcagca?ggagggagaa?gctggaaagc?agtagattct 480
gtaatgttcc?agcaactgca?aacagtagca?atgcagcatg?gcctcgtgtc?tgaggacttt 540
gaaaggcagt?tggcatatta?tgctactacc?tggacaagta?aagacatact?agaagtattg 600
gccatgatgc?ctggaaatag?agctcaaaag?gagttaattc?aagggaaatt?aaatgaagaa 660
gcagaaaggt?ggagaaggaa?taatccacca?cctccagcag?gaggaggatt?aacagtggat 720
720
<210>32
<211>597
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>32
gcagtcgatg?ctgtaatgtt?ccagcaaatg?caaacagtag?ccatgcagca?tggtcttgtg 60
tctgaggact?ttgaaaggca?gttagcatat?tgtgctacta?cctggacaag?taaagatata 120
ttagaagtat?tggccatgat?gcctggaaat?agagctcaaa?aagagttaat?tcaaggaaaa 180
ttaaacgagg?aagcagaaag?gtggagaagg?aataatccac?cgcctccaca?aggaggggga 240
ttaacagtgg?atcaaattat?ggggatagga?caaacaaatc?aagcagcagc?acaagctaac 300
atggatcagg?caagacacat?atgcctgcaa?tgggtaataa?cagcattaag?agcagtaaga 360
catatggctc?acagaccagg?gaatccaatg?ctcgtaaaac?aaaaaacaaa?tgagccatat 420
gaagagtttg?cagcaaaact?attagaagca?atagatgcag?aaccagtaac?acagcccata 480
aaagactatc?taaagttaac?attatcttat?acaaatgcgt?cctcagactg?tcaaaagcaa 540
atggatagag?tgctgggaca?aagagtgcaa?caagctagtg?tagacgagaa?aatgcaa 597
<210>33
<211>597
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>33
gcagtagact?cagtaatgtt?ccagcaactg?caaacagtag?caatgcagca?tggcctcgtg 60
tccgaggatt?ttgaaaggca?gttggcatat?tatgctacta?cctggacgag?taaagacata 120
ctagaagtat?tggccatgat?gcctggaaac?agagctcaaa?aggagttaat?tcaagggaaa 180
ttaaatgaag?aggcagaaag?gtggagaaga?cataatccac?cccctccggc?gggaggagga 240
ttaacagtgg?atcaaattat?gggggtagga?caaacaaatc?aagcagcagc?acaagctaac 300
atggatcagg?caagacaaat?atgcctgcaa?tgggtaataa?cagcattaag?agcagtgagg 360
tatatgactc?acaaaccagg?gaatccaatg?ctagtaaaac?aaaaaacaaa?tgaagcatat 420
gaagagttta?cagcgagact?gctagaagca?atagatgcag?agccagtaac?acagcccaca 480
aaagaatatc?taaaactaac?attatcttat?acaaatgcat?cctcagactg?tcaaaagcaa 540
atggatagag?tactaggaca?aagagtgcaa?caagctagtg?tagaagaaaa?aatgcaa 597
<210>34
<211>597
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>34
gcagtcgatg?ctgtaatgtt?ccagcaaatg?caaacagtag?ccatgcagca?tggtcttgtg 60
tctgaggact?ttgaaaggca?gttagcatat?tatgctacta?cctggacaag?taaagatata 120
ttagaagtat?tggccatgat?gcctggaaat?agagctcaaa?aagagttaat?tcaaggaaaa 180
ttaaatgagg?aagcagaaag?gtggagaagg?aataatccac?cgcctccaca?gggaggggga 240
ttaacagtgg?atcaaattat?ggggatagga?caaacaaatc?aagcagcagc?acaagctaac 300
atggatcagg?caagacacat?atgcctgcaa?tgggtaataa?cagcattaag?agcagtaaga 360
catatggctc?acagaccagg?gaatccaatg?ctcgtaaaac?aaaaaacaaa?tgagccatat 420
gaagagtttg?cagcaaaact?attagaagca?atagatgcag?aaccagtaac?acagctcata 480
aaagactatc?taaagttaac?attatcttat?acaaatgcgt?cctcagactg?tcaaaagcaa 540
atggatagag?tgctgggaca?aagagtgcaa?caagctagtg?tagacgagaa?gatgcaa 597
<210>35
<211>1347
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>35
atggtgagtc?tagatagaga?catggcgagg?caagtctccg?gggggaaaag?agattatcct 60
gagctcgaaa?aatgtatcaa?gcatgcatgc?aagataaaag?ttcgactcag?aggggagcac 120
ttgacagaag?gaaattgttt?atggtgcctt?aaaacattag?attacatgtt?tgaggaccat 180
aaagaggaac?cttggacaaa?agtaaaattt?aggacaatat?ggcagaaggt?gaagaatcta 240
actcctgagg?agagtaacaa?aaaagacttt?atgtctttgc?aggccacatt?agcgggtcta 300
atgtgttgcc?aaatggggat?gagacctgag?acattgcaag?atgcaatggc?tacagtaatc 360
atgaaagatg?ggttactgga?acaagaggaa?aagaaggaag?acaaaagaga?aaaggaagag 420
agtgtcttcc?caatagtagt?gcaagcagca?ggagggagaa?gctggaaagc?agtagattct 480
gtaatgttcc?agcaactgca?aacagtagca?atgcagcatg?gcctcgtgtc?tgaggacttt 540
gaaaggcagt?tggcatatta?tgctactacc?tggacaagta?aagacatact?agaagtattg 600
gccatgatgc?ctggaaatag?agctcaaaag?gagttaattc?aagggaaatt?aaatgaagaa 660
gcagaaaggt?ggagaaggaa?taatccacca?cctccagcag?gaggaggatt?aacagtggat 720
caaattatgg?gggtaggaca?aacaaatcaa?gcagcagcac?aagctaacat?ggatcaggca 780
aggcaaatat?gcctgcaatg?ggtaataaat?gcattaagag?cagtaagaca?tatggcgcac 840
aggccaggga?atccaatgct?agtaaagcaa?aaaacgaatg?agccatatga?agattttgca 900
gcaagactgc?tagaagcaat?agatgcagag?ccagttacac?agcctataaa?agattatcta 960
aagctaacac?tatcttatac?aaatgcatca?gcagattgtc?agaagcaaat?ggatagaaca 1020
ctaggacaaa?gagtacaaca?agctagtgta?gaagaaaaaa?tgcaagcatg?tagagatgtg 1080
ggatcagaag?ggttcaaaat?gcaattgtta?gcacaagcat?taaggccagg?aaaaggaaaa 1140
gggaatggac?agccacaaag?gtgttacaac?tgtggaaaac?cgggacatca?agcaaggcaa 1200
tgtagacaag?gaatcatatg?tcacaactgt?ggaaagagag?gacatatgca?aaaagaatgc 1260
agaggaaaga?gagacataag?gggaaaacag?cagggaaacg?ggaggagggg?gatacgtgtg 1320
gtgccgtccg?ctcctcctat?ggaataa 1347
<210>36
<211>597
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>36
gcagtcgatg?ctgtaatgtt?ccagcaaatg?caaacagtag?ccatgcagca?tggtcttgtg 60
tctgaggact?ttgaaaggca?gttagcatat?tgtgctacta?cctggacaag?taaagatata 120
ttagaagtat?tggccatgat?gcctggaaat?agagctcaaa?aagagttaat?tcaaggaaaa 180
ttaaacgagg?aagcagaaag?gtggagaagg?aataatccac?cgcctccaca?aggaggggga 240
ttaacagtgg?atcaaattat?ggggatagga?caaacaaatc?aagcagcagc?acaagctaac 300
atggatcagg?caagacacat?atgcctgcaa?tgggtaataa?cagcattaag?agcagtaaga 360
catatggctc?acagaccagg?gaatccaatg?ctcgtaaaac?aaaaaacaaa?tgagccatat 420
gaagagtttg?cagcaaaact?attagaagca?atagatgcag?aaccagtaac?acagcccata 480
aaagactatc?taaagttaac?attatcttat?acaaatgcgt?cctcagactg?tcaaaagcaa 540
atggatagag?tgctgggaca?aagagtgcaa?caagctagtg?tagacgagaa?aatgcaa 597
<210>37
<211>597
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>37
gcagtagact?cagtaatgtt?ccagcaactg?caaacagtag?caatgcagca?tggcctcgtg 60
tccgaggatt?ttgaaaggca?gttggcatat?tatgctacta?cctggacgag?taaagacata 120
ctagaagtat?tggccatgat?gcctggaaac?agagctcaaa?aggagttaat?tcaagggaaa 180
ttaaatgaag?aggcagaaag?gtggagaaga?cataatccac?cccctccggc?gggaggagga 240
ttaacagtgg?atcaaattat?gggggtagga?caaacaaatc?aagcagcagc?acaagctaac 300
atggatcagg?caagacaaat?atgcctgcaa?tgggtaataa?cagcattaag?agcagtgagg 360
tatatgactc?acaaaccagg?gaatccaatg?ctagtaaaac?aaaaaacaaa?tgaagcatat 420
gaagagttta?cagcgagact?gctagaagca?atagatgcag?agccagtaac?acagcccaca 480
aaagaatatc?taaaactaac?attatcttat?acaaatgcat?cctcagactg?tcaaaagcaa 540
atggatagag?tactaggaca?aagagtgcaa?caagctagtg?tagaagaaaa?aatgcaa 597
<210>38
<211>597
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>38
gcagtcgatg?ctgtaatgtt?ccagcaaatg?caaacagtag?ccatgcagca?tggtcttgtg 60
tctgaggact?ttgaaaggca?gttagcatat?tatgctacta?cctggacaag?taaagatata 120
ttagaagtat?tggccatgat?gcctggaaat?agagctcaaa?aagagttaat?tcaaggaaaa 180
ttaaatgagg?aagcagaaag?gtggagaagg?aataatccac?cgcctccaca?gggaggggga 240
ttaacagtgg?atcaaattat?ggggatagga?caaacaaatc?aagcagcagc?acaagctaac 300
atggatcagg?caagacacat?atgcctgcaa?tgggtaataa?cagcattaag?agcagtaaga 360
catatggctc?acagaccagg?gaatccaatg?ctcgtaaaac?aaaaaacaaa?tgagccatat 420
gaagagtttg?cagcaaaact?attagaagca?atagatgcag?aaccagtaac?acagctcata 480
aaagactatc?taaagttaac?attatcttat?acaaatgcgt?cctcagactg?tcaaaagcaa 540
atggatagag?tgctgggaca?aagagtgcaa?caagctagtg?tagacgagaa?gatgcaa 597
<210>39
<211>720
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>39
atggtgagtc?tagatagaga?catggcgagg?caagtctccg?gggggaaaag?agattatcct 60
gagctcgaaa?aatgtatcaa?gcatgcatgc?aagataaaag?ttcgactcag?aggggagcac 120
ttgacagaag?gaaattgttt?atggtgcctt?aaaacattag?attacatgtt?tgaggaccat 180
aaagaggaac?cttggacaaa?agtaaaattt?aggacaatat?ggcagaaggt?gaagaatcta 240
actcctgagg?agagtaacaa?aaaagacttt?atgtctttgc?aggccacatt?agcgggtcta 300
atgtgttgcc?aaatggggat?gagacctgag?acattgcaag?atgcaatggc?tacagtaatc 360
atgaaagatg?ggttactgga?acaagaggaa?aagaaggaag?acaaaagaga?aaaggaagag 420
agtgtcttcc?caatagtagt?gcaagcagca?ggagggagaa?gctggaaagc?agtagattct 480
gtaatgttcc?agcaactgca?aacagtagca?atgcagcatg?gcctcgtgtc?tgaggacttt 540
gaaaggcagt?tggcatatta?tgctactacc?tggacaagta?aagacatact?agaagtattg 600
gccatgatgc?ctggaaatag?agctcaaaag?gagttaattc?aagggaaatt?aaatgaagaa 660
gcagaaaggt?ggagaaggaa?taatccacca?cctccagcag?gaggaggatt?aacagtggat 720
720
<210>40
<211>524
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>40
taaagatata?ttagaagtat?tggccatgat?gcctggaaat?agagctcaaa?aagagttaat 60
tcaagggaaa?ttgaatgaag?aagcagaaag?gtggagaagg?aataatccac?cacctcaagc 120
aggcggagga?ttaacagtgg?atcaaattat?gggggtagga?caaacaaatc?aagcagcggc 180
acaggctaac?atggatcagg?caagacaaat?atgcctgcaa?tgggtaataa?cagcactaag 240
agcagtgaga?catatggctc?acaaaccagg?gaatccgatg?ctagtaaagc?aaaaaacaaa 300
tgagtcatat?gaagattttg?ccgcaagact?gctagaagca?atagatgcag?aaccagttac 360
aaagcaaata?aaagaatatt?taaagttaac?attatcttac?acaaatgcat?cctcagactg 420
taagaaacag?atggatagag?tactaggaca?gagagtgcaa?caagctagtg?tggaagaaaa 480
aatgcaagca?tgcagagatg?tgggatcaga?aggattcaga?atgc 524
<210>41
<211>1347
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>41
atggtgagtc?tagatagaga?catggcgagg?caagtctccg?gggggaaaag?agattatcct 60
gagctcgaaa?aatgtatcaa?gcatgcatgc?aagataaaag?ttcgactcag?aggggagcac 120
ttgacagaag?gaaattgttt?atggtgcctt?aaaacattag?attacatgtt?tgaggaccat 180
aaagaggaac?cttggacaaa?agtaaaattt?aggacaatat?ggcagaaggt?gaagaatcta 240
actcctgagg?agagtaacaa?aaaagacttt?atgtctttgc?aggccacatt?agcgggtcta 300
atgtgttgcc?aaatggggat?gagacctgag?acattgcaag?atgcaatggc?tacagtaatc 360
atgaaagatg?ggttactgga?acaagaggaa?aagaaggaag?acaaaagaga?aaaggaagag 420
agtgtcttcc?caatagtagt?gcaagcagca?ggagggagaa?gctggaaagc?agtagattct 480
gtaatgttcc?agcaactgca?aacagtagca?atgcagcatg?gcctcgtgtc?tgaggacttt 540
gaaaggcagt?tggcatatta?tgctactacc?tggacaagta?aagacatact?agaagtattg 600
gccatgatgc?ctggaaatag?agctcaaaag?gagttaattc?aagggaaatt?aaatgaagaa 660
gcagaaaggt?ggagaaggaa?taatccacca?cctccagcag?gaggaggatt?aacagtggat 720
caaattatgg?gggtaggaca?aacaaatcaa?gcagcagcac?aagctaacat?ggatcaggca 780
aggcaaatat?gcctgcaatg?ggtaataaat?gcattaagag?cagtaagaca?tatggcgcac 840
aggccaggga?atccaatgct?agtaaagcaa?aaaacgaatg?agccatatga?agattttgca 900
gcaagactgc?tagaagcaat?agatgcagag?ccagttacac?agcctataaa?agattatcta 960
aagctaacac?tatcttatac?aaatgcatca?gcagattgtc?agaagcaaat?ggatagaaca 1020
ctaggacaaa?gagtacaaca?agctagtgta?gaagaaaaaa?tgcaagcatg?tagagatgtg 1080
ggatcagaag?ggttcaaaat?gcaattgtta?gcacaagcat?taaggccagg?aaaaggaaaa 1140
gggaatggac?agccacaaag?gtgttacaac?tgtggaaaac?cgggacatca?agcaaggcaa 1200
tgtagacaag?gaatcatatg?tcacaactgt?ggaaagagag?gacatatgca?aaaagaatgc 1260
agaggaaaga?gagacataag?gggaaaacag?cagggaaacg?ggaggagggg?gatacgtgtg 1320
gtgccgtccg?ctcctcctat?ggaataa 1347
<210>42
<211>524
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>42
taaagatata?ttagaagtat?tggccatgat?gcctggaaat?agagctcaaa?aagagttaat 60
tcaagggaaa?ttgaatgaag?aagcagaaag?gtggagaagg?aataatccac?cacctcaagc 120
aggcggagga?ttaacagtgg?atcaaattat?gggggtagga?caaacaaatc?aagcagcggc 180
acaggctaac?atggatcagg?caagacaaat?atgcctgcaa?tgggtaataa?cagcactaag 240
agcagtgaga?catatggctc?acaaaccagg?gaatccgatg?ctagtaaagc?aaaaaacaaa 300
tgagtcatat?gaagattttg?ccgcaagact?gctagaagca?atagatgcag?aaccagttac 360
aaagcaaata?aaagaatatt?taaagttaac?attatcttac?acaaatgcat?cctcagactg 420
taagaaacag?atggatagag?tactaggaca?gagagtgcaa?caagctagtg?tggaagaaaa 480
aatgcaagca?tgcagagatg?tgggatcaga?aggattcaga?atgc 524
<210>43
<211>720
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>43
atggtgagtc?tagatagaga?catggcgagg?caagtctccg?gggggaaaag?agattatcct 60
gagctcgaaa?aatgtatcaa?gcatgcatgc?aagataaaag?ttcgactcag?aggggagcac 120
ttgacagaag?gaaattgttt?atggtgcctt?aaaacattag?attacatgtt?tgaggaccat 180
aaagaggaac?cttggacaaa?agtaaaattt?aggacaatat?ggcagaaggt?gaagaatcta 240
actcctgagg?agagtaacaa?aaaagacttt?atgtctttgc?aggccacatt?agcgggtcta 300
atgtgttgcc?aaatggggat?gagacctgag?acattgcaag?atgcaatggc?tacagtaatc 360
atgaaagatg?ggttactgga?acaagaggaa?aagaaggaag?acaaaagaga?aaaggaagag 420
agtgtcttcc?caatagtagt?gcaagcagca?ggagggagaa?gctggaaagc?agtagattct 480
gtaatgttcc?agcaactgca?aacagtagca?atgcagcatg?gcctcgtgtc?tgaggacttt 540
gaaaggcagt?tggcatatta?tgctactacc?tggacaagta?aagacatact?agaagtattg 600
gccatgatgc?ctggaaatag?agctcaaaag?gagttaattc?aagggaaatt?aaatgaagaa 660
gcagaaaggt?ggagaaggaa?taatccacca?cctccagcag?gaggaggatt?aacagtggat 720
720
<210>44
<211>593
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>44
tgccgtagac?tctgtgatgt?tccaccagct?gcatacagta?gcaatgccgc?atggcctcgt 60
gtctgaggac?tttgaaaggc?agttggcata?ttatgctact?acctggacaa?gtaaagatat 120
actggaagta?ttggccatga?tgcctgggaa?tagagctcaa?aaagaattaa?ttcaaggaaa 180
attaaatgaa?gaagcagaaa?ggtggagaag?gaataatcca?ccacctcaag?caggcggagg 240
attaacagtg?gatcaaatta?tgggggtagg?acaaacaaat?caagcagctg?cacaagctaa 300
catggatcag?gcaagacaaa?tatgcctgca?atgggtaata?tcagccttaa?gagcagtgag 360
acatatgtct?cataaaccag?ggaatccgct?gctagtaaag?caaaaaacaa?atgagtcata 420
tgaagatttt?gcagctagac?tgctagaagc?aatagatcca?gccccagtag?cacatcctat 480
aaaagattat?ttaaagttaa?cactatctta?tacgaatgca?tcatcagatt?gtcaaaagca 540
aatgggtaga?atgctaggat?cgagagtcca?tcaagccagt?gtgggccaaa?aaa 593
<210>45
<211>1347
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>45
atggtgagtc?tagatagaga?catggcgagg?caagtctccg?gggggaaaag?agattatcct 60
gagctcgaaa?aatgtatcaa?gcatgcatgc?aagataaaag?ttcgactcag?aggggagcac 120
ttgacagaag?gaaattgttt?atggtgcctt?aaaacattag?attacatgtt?tgaggaccat 180
aaagaggaac?cttggacaaa?agtaaaattt?aggacaatat?ggcagaaggt?gaagaatcta 240
actcctgagg?agagtaacaa?aaaagacttt?atgtctttgc?aggccacatt?agcgggtcta 300
atgtgttgcc?aaatggggat?gagacctgag?acattgcaag?atgcaatggc?tacagtaatc 360
atgaaagatg?ggttactgga?acaagaggaa?aagaaggaag?acaaaagaga?aaaggaagag 420
agtgtcttcc?caatagtagt?gcaagcagca?ggagggagaa?gctggaaagc?agtagattct 480
gtaatgttcc?agcaactgca?aacagtagca?atgcagcatg?gcctcgtgtc?tgaggacttt 540
gaaaggcagt?tggcatatta?tgctactacc?tggacaagta?aagacatact?agaagtattg 600
gccatgatgc?ctggaaatag?agctcaaaag?gagttaattc?aagggaaatt?aaatgaagaa 660
gcagaaaggt?ggagaaggaa?taatccacca?cctccagcag?gaggaggatt?aacagtggat 720
caaattatgg?gggtaggaca?aacaaatcaa?gcagcagcac?aagctaacat?ggatcaggca 780
aggcaaatat?gcctgcaatg?ggtaataaat?gcattaagag?cagtaagaca?tatggcgcac 840
aggccaggga?atccaatgct?agtaaagcaa?aaaacgaatg?agccatatga?agattttgca 900
gcaagactgc?tagaagcaat?agatgcagag?ccagttacac?agcctataaa?agattatcta 960
aagctaacac?tatcttatac?aaatgcatca?gcagattgtc?agaagcaaat?ggatagaaca 1020
ctaggacaaa?gagtacaaca?agctagtgta?gaagaaaaaa?tgcaagcatg?tagagatgtg 1080
ggatcagaag?ggttcaaaat?gcaattgtta?gcacaagcat?taaggccagg?aaaaggaaaa 1140
gggaatggac?agccacaaag?gtgttacaac?tgtggaaaac?cgggacatca?agcaaggcaa 1200
tgtagacaag?gaatcatatg?tcacaactgt?ggaaagagag?gacatatgca?aaaagaatgc 1260
agaggaaaga?gagacataag?gggaaaacag?cagggaaacg?ggaggagggg?gatacgtgtg 1320
gtgccgtccg?ctcctcctat?ggaataa 1347
<210>46
<211>593
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>46
tgccgtagac?tctgtgatgt?tccaccagct?gcatacagta?gcaatgccgc?atggcctcgt 60
gtctgaggac?tttgaaaggc?agttggcata?ttatgctact?acctggacaa?gtaaagatat 120
actggaagta?ttggccatga?tgcctgggaa?tagagctcaa?aaagaattaa?ttcaaggaaa 180
attaaatgaa?gaagcagaaa?ggtggagaag?gaataatcca?ccacctcaag?caggcggagg 240
attaacagtg?gatcaaatta?tgggggtagg?acaaacaaat?caagcagctg?cacaagctaa 300
catggatcag?gcaagacaaa?tatgcctgca?atgggtaata?tcagccttaa?gagcagtgag 360
acatatgtct?cataaaccag?ggaatccgct?gctagtaaag?caaaaaacaa?atgagtcata 420
tgaagatttt?gcagctagac?tgctagaagc?aatagatcca?gccccagtag?cacatcctat 480
aaaagattat?ttaaagttaa?cactatctta?tacgaatgca?tcatcagatt?gtcaaaagca 540
aatgggtaga?atgctaggat?cgagagtcca?tcaagccagt?gtgggccaaa?aaa 593
<210>47
<211>720
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>47
atggtgagtc?tagatagaga?catggcgagg?caagtctccg?gggggaaaag?agattatcct 60
gagctcgaaa?aatgtatcaa?gcatgcatgc?aagataaaag?ttcgactcag?aggggagcac 120
ttgacagaag?gaaattgttt?atggtgcctt?aaaacattag?attacatgtt?tgaggaccat 180
aaagaggaac?cttggacaaa?agtaaaattt?aggacaatat?ggcagaaggt?gaagaatcta 240
actcctgagg?agagtaacaa?aaaagacttt?atgtctttgc?aggccacatt?agcgggtcta 300
atgtgttgcc?aaatggggat?gagacctgag?acattgcaag?atgcaatggc?tacagtaatc 360
atgaaagatg?ggttactgga?acaagaggaa?aagaaggaag?acaaaagaga?aaaggaagag 420
agtgtcttcc?caatagtagt?gcaagcagca?ggagggagaa?gctggaaagc?agtagattct 480
gtaatgttcc?agcaactgca?aacagtagca?atgcagcatg?gcctcgtgtc?tgaggacttt 540
gaaaggcagt?tggcatatta?tgctactacc?tggacaagta?aagacatact?agaagtattg 600
gccatgatgc?ctggaaatag?agctcaaaag?gagttaattc?aagggaaatt?aaatgaagaa 660
gcagaaaggt?ggagaaggaa?taatccacca?cctccagcag?gaggaggatt?aacagtggat 720
720
<210>48
<211>593
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>48
agcagtagat?tctgtaatgt?tccagcaact?gcaaacagta?gcaatgcagc?atggactcgt 60
gtatgaagac?tttgaaaggc?tgtcggcata?ttatgctact?acctggacaa?gtaaagatat 120
actggaagta?ttggccatga?tgcctgggaa?tagagctcaa?aaagaattaa?ttcaaggaaa 180
attaaatgaa?gaagcagaaa?ggtggagaag?gaataatcca?ccacctcaag?caggcggagg 240
attaacagtg?gatcaaatta?tgggggtagg?acaaacaaat?caagcagctg?cacaagctaa 300
catggatcag?gcaagacaaa?tatgcctgca?atgggtaata?tcagccttaa?gagcagtgag 360
acatatgtct?cataaaccag?ggaatccgct?gctagtaaag?caaaaaacaa?atgagtcata 420
tgaagatttt?gcagcaagac?tgctagaagc?aatagatgca?gagccagtag?cacatcctat 480
aaaagaatac?ttaaagttaa?cactatctta?tacgaatgca?tcatcagatt?gtcaaaagca 540
aatggataga?atgctggaat?caagagtaca?acaagctagt?gtagaacaaa?aaa 593
<210>49
<211>593
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>49
agccgtagat?tctgtaatgt?tccagcagct?gcaaacagta?gcaatgcagc?atggcctcgt 60
gtcagaggac?tttgaaaggc?ttccagcata?tcatgctact?acctgggcaa?gtaaagatat 120
cttagaagta?ctggccatga?tgcctggaaa?tagagctcaa?aaagagttaa?ttcaagggaa 180
attaaatgaa?gaagcagaga?ggtggagaag?gaataatcca?ccacctccag?caggaggagg 240
gttaacagtg?gatcaaatta?tgggagtagg?acaaacaaat?caggcagcgg?cacaagcaaa 300
catggatcag?gcaagacaaa?tatgcctaca?atgggtgata?tcagcactaa?gagcagtaag 360
gcatatggct?cacaagccag?ggaatccaat?gttagtaaag?caaaaagcaa?atgagccata 420
tgaagaattt?gcagcaaggc?tgctggaagc?aatagatgcc?gagccagtta?atcagcccat 480
aaaagaatat?ctaaaactaa?cgttgtctta?tacgaatgca?tcctcagatt?gtcagaagca 540
aatggataga?acactaggac?aaagagtcaa?acaagctagt?gtagaacaaa?aaa 593
<210>50
<211>1347
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>50
atggtgagtc?tagatagaga?catggcgagg?caagtctccg?gggggaaaag?agattatcct 60
gagctcgaaa?aatgtatcaa?gcatgcatgc?aagataaaag?ttcgactcag?aggggagcac 120
ttgacagaag?gaaattgttt?atggtgcctt?aaaacattag?attacatgtt?tgaggaccat 180
aaagaggaac?cttggacaaa?agtaaaattt?aggacaatat?ggcagaaggt?gaagaatcta 240
actcctgagg?agagtaacaa?aaaagacttt?atgtctttgc?aggccacatt?agcgggtcta 300
atgtgttgcc?aaatggggat?gagacctgag?acattgcaag?atgcaatggc?tacagtaatc 360
atgaaagatg?ggttactgga?acaagaggaa?aagaaggaag?acaaaagaga?aaaggaagag 420
agtgtcttcc?caatagtagt?gcaagcagca?ggagggagaa?gctggaaagc?agtagattct 480
gtaatgttcc?agcaactgca?aacagtagca?atgcagcatg?gcctcgtgtc?tgaggacttt 540
gaaaggcagt?tggcatatta?tgctactacc?tggacaagta?aagacatact?agaagtattg 600
gccatgatgc?ctggaaatag?agctcaaaag?gagttaattc?aagggaaatt?aaatgaagaa 660
gcagaaaggt?ggagaaggaa?taatccacca?cctccagcag?gaggaggatt?aacagtggat 720
caaattatgg?gggtaggaca?aacaaatcaa?gcagcagcac?aagctaacat?ggatcaggca 780
aggcaaatat?gcctgcaatg?ggtaataaat?gcattaagag?cagtaagaca?tatggcgcac 840
aggccaggga?atccaatgct?agtaaagcaa?aaaacgaatg?agccatatga?agattttgca 900
gcaagactgc?tagaagcaat?agatgcagag?ccagttacac?agcctataaa?agattatcta 960
aagctaacac?tatcttatac?aaatgcatca?gcagattgtc?agaagcaaat?ggatagaaca 1020
ctaggacaaa?gagtacaaca?agctagtgta?gaagaaaaaa?tgcaagcatg?tagagatgtg 1080
ggatcagaag?ggttcaaaat?gcaattgtta?gcacaagcat?taaggccagg?aaaaggaaaa 1140
gggaatggac?agccacaaag?gtgttacaac?tgtggaaaac?cgggacatca?agcaaggcaa 1200
tgtagacaag?gaatcatatg?tcacaactgt?ggaaagagag?gacatatgca?aaaagaatgc 1260
agaggaaaga?gagacataag?gggaaaacag?cagggaaacg?ggaggagggg?gatacgtgtg 1320
gtgccgtccg?ctcctcctat?ggaataa 1347
<210>51
<211>593
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>51
agcagtagat?tctgtaatgt?tccagcaact?gcaaacagta?gcaatgcagc?atggactcgt 60
gtatgaagac?tttgaaaggc?tgtcggcata?ttatgctact?acctggacaa?gtaaagatat 120
actggaagta?ttggccatga?tgcctgggaa?tagagctcaa?aaagaattaa?ttcaaggaaa 180
attaaatgaa?gaagcagaaa?ggtggagaag?gaataatcca?ccacctcaag?caggcggagg 240
attaacagtg?gatcaaatta?tgggggtagg?acaaacaaat?caagcagctg?cacaagctaa 300
catggatcag?gcaagacaaa?tatgcctgca?atgggtaata?tcagccttaa?gagcagtgag 360
acatatgtct?cataaaccag?ggaatccgct?gctagtaaag?caaaaaacaa?atgagtcata 420
tgaagatttt?gcagcaagac?tgctagaagc?aatagatgca?gagccagtag?cacatcctat 480
aaaagaatat?ctaaaactaa?cgttgtctta?tacgaatgca?tcctcagatt?gtcagaagca 540
aatggataga?acactaggac?aaagagtcaa?acaagctagt?gtagaacaaa?aaa 593
<210>52
<211>593
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>52
agccgtagat?tctgtaatgt?tccagcagct?gcaaacagta?gcaatgcagc?atggcctcgt 60
gtcagaggac?tttgaaaggc?ttccagcata?tcatgctact?acctgggcaa?gtaaagatat 120
cttagaagta?ctggccatga?tgcctggaaa?tagagctcaa?aaagagttaa?ttcaagggaa 180
attaaatgaa?gaagcagaga?ggtggagaag?gaataatcca?ccacctccag?caggaggagg 240
gttaacagtg?gatcaaatta?tgggagtagg?acaaacaaat?caggcagcgg?cacaagcaaa 300
catggatcag?gcaagacaaa?tatgcctaca?atgggtgata?tcagcactaa?gagcagtaag 360
gcatatggct?cacaagccag?ggaatccaat?gttagtaaag?caaaaagcaa?atgagccata 420
tgaagaattt?gcagcaaggc?tgctggaagc?aatagatgcc?gagccagtta?atcagcccat 480
aaaagaatat?ctaaaactaa?cgttgtctta?tacgaatgca?tcctcagatt?gtcagaagca 540
aatggataga?acactaggac?aaagagtcaa?acaagctagt?gtagaacaaa?aaa 593
<210>53
<211>720
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>53
atggtgagtc?tagatagaga?catggcgagg?caagtctccg?gggggaaaag?agattatcct 60
gagctcgaaa?aatgtatcaa?gcatgcatgc?aagataaaag?ttcgactcag?aggggagcac 120
ttgacagaag?gaaattgttt?atggtgcctt?attacatgtt?tgaggaccat?aaagaggaac 180
cttggacaaa?aaaacattag?agtaaaattt?aggacaatat?ggcagaaggt?gaagaatcta 240
actcctgagg?agagtaacaa?aaaagacttt?atgtctttgc?aggccacatt?agcgggtcta 300
atgtgttgcc?aaatggggat?gagacctgag?acattgcaag?atgcaatggc?tacagtaatc 360
atgaaagatg?ggttactgga?acaagaggaa?aagaaggaag?acaaaagaga?aaaggaagag 420
agtgtcttcc?caatagtagt?gcaagcagca?ggagggagaa?gctggaaagc?agtagattct 480
gtaatgttcc?agcaactgca?aacagtagca?atgcagcatg?gcctcgtgtc?tgaggacttt 540
gaaaggcagt?tggcatatta?tgctactacc?tggacaagta?aagacatact?agaagtattg 600
gccatgatgc?ctggaaatag?agctcaaaag?gagttaattc?aagggaaatt?aaatgaagaa 660
gcagaaaggt?ggagaaggaa?taatccacca?cctccagcag?gaggaggatt?aacagtggat 720
720
<210>54
<211>129
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>54
cagcatggcc?tcgtgtccga?ggactttgaa?aggcagttgg?catattatgc?tactacctgg 60
acaagtaagg?acatattaga?agtattggcc?atgatgccag?gaaatagagc?tcaaaaggag 120
ctaattcaa 129
<210>55
<211>129
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>55
cagcatggaa?tagtatcaga?agagtttgag?aggcaactgt?cttattatgc?taccacttgg 60
acaagcaagg?atatcttaga?ggtactagcc?atgatgcctg?gcaatagagc?attaaaagag 120
ctaatacaa 129
<210>56
<211>129
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>56
caacatggga?tagtatcaga?ggaatttgag?agacaaatgt?cttattatgc?taccacatgg 60
acaagtaagg?atattttaga?agtactagca?atgatgcccg?ggaacagagc?attaaaggag 120
ctgatacaa 129
<210>57
<211>129
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>57
cagcatggac?tagtatcaga?agaatttgaa?aggcagctag?catactatgc?cacaacgtgg 60
acaagcaaag?acatactaga?ggtgttagcc?atgatgccag?ggaatagagc?acaaaaagaa 120
ctaatacaa 129
<210>58
<211>129
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>58
cagcatggac?ttgtgtccga?agattttgag?aggcaattgg?catattatgc?tacaacctgg 60
actagtgaag?atatattaga?agtattggct?atgatgcctg?ggaatagagc?acagaaagaa 120
ttaatacaa 129
<210>59
<211>1347
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>59
atggtgagtc?tagatagaga?catggcgagg?caagtctccg?gggggaaaag?agattatcct 60
gagctcgaaa?aatgtatcaa?gcatgcatgc?aagataaaag?ttcgactcag?aggggagcac 120
ttgacagaag?gaaattgttt?atggtgcctt?aaaacattag?attacatgtt?tgaggaccat 180
aaagaggaac?cttggacaaa?agtaaaattt?aggacaatat?ggcagaaggt?gaagaatcta 240
actcctgagg?agagtaacaa?aaaagacttt?atgtctttgc?aggccacatt?agcgggtcta 300
atgtgttgcc?aaatggggat?gagacctgag?acattgcaag?atgcaatggc?tacagtaatc 360
atgaaagatg?ggttactgga?acaagaggaa?aagaaggaag?acaaaagaga?aaaggaagag 420
agtgtcttcc?caatagtagt?gcaagcagca?ggagggagaa?gctggaaagc?agtagattct 480
gtaatgttcc?agcaactgca?aacagtagca?atgcagcatg?gcctcgtgtc?tgaggacttt 540
gaaaggcagt?tggcatatta?tgctactacc?tggacaagta?aagacatact?agaagtattg 600
gccatgatgc?ctggaaatag?agctcaaaag?gagttaattc?aagggaaatt?aaatgaagaa 660
gcagaaaggt?ggagaaggaa?taatccacca?cctccagcag?gaggaggatt?aacagtggat 720
caaattatgg?gggtaggaca?aacaaatcaa?gcagcagcac?aagctaacat?ggatcaggca 780
aggcaaatat?gcctgcaatg?ggtaataaat?gcattaagag?cagtaagaca?tatggcgcac 840
aggccaggga?atccaatgct?agtaaagcaa?aaaacgaatg?agccatatga?agattttgca 900
gcaagactgc?tagaagcaat?agatgcagag?ccagttacac?agcctataaa?agattatcta 960
aagctaacac?tatcttatac?aaatgcatca?gcagattgtc?agaagcaaat?ggatagaaca 1020
ctaggacaaa?gagtacaaca?agctagtgta?gaagaaaaaa?tgcaagcatg?tagagatgtg 1080
ggatcagaag?ggttcaaaat?gcaattgtta?gcacaagcat?taaggccagg?aaaaggaaaa 1140
gggaatggac?agccacaaag?gtgttacaac?tgtggaaaac?cgggacatca?agcaaggcaa 1200
tgtagacaag?gaatcatatg?tcacaactgt?ggaaagagag?gacatatgca?aaaagaatgc 1260
agaggaaaga?gagacataag?gggaaaacag?cagggaaacg?ggaggagggg?gatacgtgtg 1320
gtgccgtccg?ctcctcctat?ggaataa 1347
<210>60
<211>129
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>60
cagcatggcc?tcgtgtccga?ggactttgaa?aggcagttgg?catattatgc?tactacctgg 60
acaagtaagg?acatattaga?agtattggcc?atgatgccag?gaaatagagc?tcaaaaggag 120
ctaattcaa 129
<210>61
<211>129
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>61
cagcatggaa?tagtatcaga?agagtttgag?aggcaactgt?cttattatgc?taccacttgg 60
acaagcaagg?atatcttaga?ggtactagcc?atgatgcctg?gcaatagagc?attaaaagag 120
ctaatacaa 129
<210>62
<211>129
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>62
caacatggga?tagtatcaga?ggaatttgag?agacaaatgt?cttattatgc?taccacatgg 60
acaagtaagg?atattttaga?agtactagca?atgatgcccg?ggaacagagc?attaaaggag 120
ctgatacaa 129
<210>63
<211>129
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>63
cagcatggac?tagtatcaga?agaatttgaa?aggcagctag?catactatgc?cacaacgtgg 60
acaagcaaag?acatactaga?ggtgttagcc?atgatgccag?ggaatagagc?acaaaaagaa 120
ctaatacaa 129
<210>64
<211>129
<212>DNA
＜213〉arthritis-Encephalitis virus
<400>64
cagcatggac?ttgtgtccga?agattttgag?aggcaattgg?catattatgc?tacaacctgg 60
actagtgaag?atatattaga?agtattggct?atgatgcctg?ggaatagagc?acagaaagaa 120
ttaatacaa 129
<210>65
<211>20
<212>DNA
＜213〉artificial sequence
<220>
＜223〉be used to make up the primer of dig-label probe
<400>65
ctggcgtaat?agcgaagagg 20
<210>66
<211>20
<212>DNA
＜213〉artificial sequence
＜220〉be used to make up the primer of dig-label probe
<223>Primer?used?to?construct?dig-labeled?probe
<400>66
aactcgccgc?acatctgaac 20
<210>67
<211>3911
<212>DNA
＜213〉artificial sequence
<220>
<223>pCAH/SINd0
<400>67
atccagcaca?gtggcggccg?ctagcacaaa?aataaaaaaa?gaaagggtgg?actgtgagac 60
atgggctaaa?gaggagcggc?cgctcgagtc?tagaactagt?ggatcagctt?tgctgcttgc 120
acttcagagt?tctaggagag?tccctcctag?tctctcctct?ccgaggaggt?accgagacct 180
caaaataaag?gagtgattgc?cttactgccg?agtggagagt?gattactgag?cggccggtgt 240
atcgggagtc?gtcccttaat?ctgtgcaata?ccagagcggc?tctcgcagcc?gacctcgagg 300
gggggcccta?ttctatagtg?tcacctaaat?gctagagctc?gctgatcagc?ctcgactgtg 360
ccttctagtt?gccagccatc?tgttgtttgc?ccctcccccg?tgccttcctt?gaccctggaa 420
ggtgccactc?ccactgtcct?ttcctaataa?aatgaggaaa?ttgcatcgca?ttgtctgagt 480
aggtgtcatt?ctattctggg?gggtggggtg?gggcaggaca?gcaaggggga?ggattgggaa 540
gacaatagca?ggcatgctgg?ggatgcggtg?ggctctatgg?cttctgaggc?ggaaagaacc 600
agtggcggta?atacggttat?ccacagaatc?aggggataac?gcaggaaaga?acatgtgagc 660
aaaaggccag?caaaaggcca?ggaaccgtaa?aaaggccgcg?ttgctggcgt?ttttccatag 720
gctccgcccc?cctgacgagc?atcacaaaaa?tcgacgctca?agtcagaggt?ggcgaaaccc 780
gacaggacta?taaagatacc?aggcgtttcc?ccctggaagc?tccctcgtgc?gctctcctgt 840
tccgaccctg?ccgcttaccg?gatacctgtc?cgcctttctc?ccttcgggaa?gcgtggcgct 900
ttctcatagc?tcacgctgta?ggtatctcag?ttcggtgtag?gtcgttcgct?ccaagctggg 960
ctgtgtgcac?gaaccccccg?ttcagcccga?ccgctgcgcc?ttatccggta?actatcgtct 1020
tgagtccaac?ccggtaagac?acgacttatc?gccactggca?gcagccactg?gtaacaggat 1080
tagcagagcg?aggtatgtag?gcggtgctac?agagttcttg?aagtggtggc?ctaactacgg 1140
ctacactaga?aggacagtat?ttggtatctg?cgctctgctg?aagccagtta?ccttcggaaa 1200
aagagttggt?agctcttgat?ccggcaaaca?aaccaccgct?ggtagcggtg?gtttttttgt 1260
ttgcaagcag?cagattacgc?gcagaaaaaa?aggatctcaa?gaagatcctt?tgatcttttc 1320
tacggggtct?gacgctcagt?ggaacgaaaa?ctcacgttaa?gggattttgg?tcatgagatt 1380
atcaaaaagg?atcttcacct?agatcctttt?aaattaaaaa?tgaagtttta?aatcaatcta 1440
aagtatatat?gagtaacctg?atcaggactc?ttccttttca?tgaacaataa?aactgtctgc 1500
ttacataaac?agtaatacaa?ggggtgttat?gagccatatt?caacgggaaa?cgtcttgctc 1560
taggccgcga?ttaaattcca?acatggatgc?tgatttatat?gggtataaat?gggctcgcga 1620
taatgtcggg?caatcaggtg?cgacaatcta?tcgattgtat?gggaagcccg?atgcgccaga 1680
gttgtttctg?aaacatggca?aaggtagcgt?tgccaatgat?gttacagatg?agatggtcag 1740
actaaactgg?ctgacggaat?ttatgcctct?tccgaccatc?aagcatttta?tccgtactcc 1800
tgatgatgca?tggttactca?ccactgcgat?ccccgggaaa?acagcattcc?aggtattaga 1860
agaatatcct?gattcaggtg?aaaatattgt?tgatgcgctg?gcagtgttcc?tgcgccggtt 1920
gcattcgatt?cctgtttgta?attgtccttt?taacagcgat?cgcgtatttc?gtctcgctca 1980
ggcgcaatca?cgaatgaata?acggtttggt?tgatgcgagt?gattttgatg?acgagcgtaa 2040
tggctggcct?gttgaacaag?tctggaaaga?aatgcataaa?cttttgccat?tctcaccgga 2100
ttcagtcgtc?actcatggtg?atttctcact?tgataacctt?atttttgacg?aggggaaatt 2160
aataggttgt?attgatgttg?gacgagtcgg?aatcgcagac?cgataccagg?atcttgccat 2220
cctatggaac?tgcctcggtg?agttttctcc?ttcattacag?aaacggcttt?ttcaaaaata 2280
tggtattgat?aatcctgata?tgaataaatt?gcagtttcat?ttgatgctcg?atgagttttt 2340
ctaagaattc?gcgcaattaa?ccctcactaa?agggaacaaa?agctgggtac?cgggcccgtt 2400
gacattgatt?attgactagt?tattaatagt?aatcaattac?ggggtcatta?gttcatagcc 2460
catatatgga?gttccgcgtt?acataactta?cggtaaatgg?cccgcctggc?tgaccgccca 2520
acgacccccg?cccattgacg?tcaataatga?cgtatgttcc?catagtaacg?ccaataggga 2580
ctttccattg?acgtcaatgg?gtggagtatt?tacggtaaac?tgcccacttg?gcagtacatc 2640
aagtgtatca?tatgccaagt?acgcccccta?ttgacgtcaa?tgacggtaaa?tggcccgcct 2700
ggcattatgc?ccagtacatg?accttatggg?actttcctac?ttggcagtac?atctacgtat 2760
tagtcatcgc?tattaccatg?gtgatgcggt?tttggcagta?catcaatggg?cgtggatagc 2820
ggtttgactc?acggggattt?ccaagtctcc?accccattga?cgtcaatggg?agtttgtttt 2880
ggcaccaaaa?tcaacgggac?tttccaaaat?gtcgtaacaa?ctccgcccca?ttgacgcaaa 2940
tgggcggtag?gcgtgtacgg?tgggaggtct?atataagcag?agctcaagct?tgctgcttgc 3000
acttcagagt?tctaggagag?tccctcctag?tctctcctct?ccgaggaggt?accgagacct 3060
caaaataaag?gagtgattgc?cttactgccg?agtggagagt?gattactgag?cggccggtgt 3120
atcgggagtc?gtcccttaat?ctgtgcaata?ccagagcggc?tctcgcagct?ggcgcccaac 3180
gtggggcccg?aggagaagaa?aagaaagcgg?ccctgagaac?tcggcttctg?aaaaagagga 3240
agaggacaag?ttgctatagc?aacaagagag?aagaagtaga?gcaaaggtcc?agtggctcgg 3300
aaaaagagga?actgaaactt?cggggacgcc?tgaaggagta?aggtaagtga?ctctgctgta 3360
cgcggggcga?ggcagaggtt?tccttctaaa?ttgaaagaga?agtgttgctg?cgagaggtct 3420
tggtggtcga?gaatcctgta?caaaaaaaag?gagggatctc?ggtcaggacc?aggacccctg 3480
ggagtaatac?aacagcaaca?ccgtaagaaa?atccgcctag?ggaattcgat?tctagaggtg 3540
atagaaatgc?cagaaaacta?tgcaaaaaca?agaatcataa?acaggaaaaa?aagagaactc 3600
agccacaaga?ggaagaagag?aggcgttggc?ttggtcatta?tgctagttat?catggcaata 3660
gtagctgccg?caggggcttc?tctgggagtc?gcaaacgcga?ttcagcagtc?ttacactaag 3720
gcagctgtcc?agacccttgc?taatgcaact?gctgcacagc?aggatgtgtt?agaggcaacc 3780
tatgccatgg?tacagcatgt?ggctaaaggc?gtacgaatct?tggaagctcg?agtggctcga 3840
gtggaagcta?tcacagatag?aataatgcta?taccaagaat?tggattgttg?gcactaggat 3900
ccatcgccac?c 3911
<210>68
<211>4238
<212>DNA
＜213〉artificial sequence
<220>
<223>pCAH/SINd1
<400>68
atccagcaca?gtggcggccg?ctagcacaaa?aataaaaaaa?gaaagggtgg?actgtgagac 60
atgggctaaa?gaggagcggc?cgctcgagtc?tagaactagt?ggatcagctt?tgctgcttgc 120
acttcagagt?tctaggagag?tccctcctag?tctctcctct?ccgaggaggt?accgagacct 180
caaaataaag?gagtgattgc?cttactgccg?agtggagagt?gattactgag?cggccggtgt 240
atcgggagtc?gtcccttaat?ctgtgcaata?ccagagcggc?tctcgcagcc?gacctcgagg 300
gggggcccta?ttctatagtg?tcacctaaat?gctagagctc?gctgatcagc?ctcgactgtg 360
ccttctagtt?gccagccatc?tgttgtttgc?ccctcccccg?tgccttcctt?gaccctggaa 420
ggtgccactc?ccactgtcct?ttcctaataa?aatgaggaaa?ttgcatcgca?ttgtctgagt 480
aggtgtcatt?ctattctggg?gggtggggtg?gggcaggaca?gcaaggggga?ggattgggaa 540
gacaatagca?ggcatgctgg?ggatgcggtg?ggctctatgg?cttctgaggc?ggaaagaacc 600
agtggcggta?atacggttat?ccacagaatc?aggggataac?gcaggaaaga?acatgtgagc 660
aaaaggccag?caaaaggcca?ggaaccgtaa?aaaggccgcg?ttgctggcgt?ttttccatag 720
gctccgcccc?cctgacgagc?atcacaaaaa?tcgacgctca?agtcagaggt?ggcgaaaccc 780
gacaggacta?taaagatacc?aggcgtttcc?ccctggaagc?tccctcgtgc?gctctcctgt 840
tccgaccctg?ccgcttaccg?gatacctgtc?cgcctttctc?ccttcgggaa?gcgtggcgct 900
ttctcatagc?tcacgctgta?ggtatctcag?ttcggtgtag?gtcgttcgct?ccaagctggg 960
ctgtgtgcac?gaaccccccg?ttcagcccga?ccgctgcgcc?ttatccggta?actatcgtct 1020
tgagtccaac?ccggtaagac?acgacttatc?gccactggca?gcagccactg?gtaacaggat 1080
tagcagagcg?aggtatgtag?gcggtgctac?agagttcttg?aagtggtggc?ctaactacgg 1140
ctacactaga?aggacagtat?ttggtatctg?cgctctgctg?aagccagtta?ccttcggaaa 1200
aagagttggt?agctcttgat?ccggcaaaca?aaccaccgct?ggtagcggtg?gtttttttgt 1260
ttgcaagcag?cagattacgc?gcagaaaaaa?aggatctcaa?gaagatcctt?tgatcttttc 1320
tacggggtct?gacgctcagt?ggaacgaaaa?ctcacgttaa?gggattttgg?tcatgagatt 1380
atcaaaaagg?atcttcacct?agatcctttt?aaattaaaaa?tgaagtttta?aatcaatcta 1440
aagtatatat?gagtaacctg?atcaggactc?ttccttttca?tgaacaataa?aactgtctgc 1500
ttacataaac?agtaatacaa?ggggtgttat?gagccatatt?caacgggaaa?cgtcttgctc 1560
taggccgcga?ttaaattcca?acatggatgc?tgatttatat?gggtataaat?gggctcgcga 1620
taatgtcggg?caatcaggtg?cgacaatcta?tcgattgtat?gggaagcccg?atgcgccaga 1680
gttgtttctg?aaacatggca?aaggtagcgt?tgccaatgat?gttacagatg?agatggtcag 1740
actaaactgg?ctgacggaat?ttatgcctct?tccgaccatc?aagcatttta?tccgtactcc 1800
tgatgatgca?tggttactca?ccactgcgat?ccccgggaaa?acagcattcc?aggtattaga 1860
agaatatcct?gattcaggtg?aaaatattgt?tgatgcgctg?gcagtgttcc?tgcgccggtt 1920
gcattcgatt?cctgtttgta?attgtccttt?taacagcgat?cgcgtatttc?gtctcgctca 1980
ggcgcaatca?cgaatgaata?acggtttggt?tgatgcgagt?gattttgatg?acgagcgtaa 2040
tggctggcct?gttgaacaag?tctggaaaga?aatgcataaa?cttttgccat?tctcaccgga 2100
ttcagtcgtc?actcatggtg?atttctcact?tgataacctt?atttttgacg?aggggaaatt 2160
aataggttgt?attgatgttg?gacgagtcgg?aatcgcagac?cgataccagg?atcttgccat 2220
cctatggaac?tgcctcggtg?agttttctcc?ttcattacag?aaacggcttt?ttcaaaaata 2280
tggtattgat?aatcctgata?tgaataaatt?gcagtttcat?ttgatgctcg?atgagttttt 2340
ctaagaattc?gcgcaattaa?ccctcactaa?agggaacaaa?agctgggtac?cgggcccgtt 2400
gacattgatt?attgactagt?tattaatagt?aatcaattac?ggggtcatta?gttcatagcc 2460
catatatgga?gttccgcgtt?acataactta?cggtaaatgg?cccgcctggc?tgaccgccca 2520
acgacccccg?cccattgacg?tcaataatga?cgtatgttcc?catagtaacg?ccaataggga 2580
ctttccattg?acgtcaatgg?gtggagtatt?tacggtaaac?tgcccacttg?gcagtacatc 2640
aagtgtatca?tatgccaagt?acgcccccta?ttgacgtcaa?tgacggtaaa?tggcccgcct 2700
ggcattatgc?ccagtacatg?accttatggg?actttcctac?ttggcagtac?atctacgtat 2760
tagtcatcgc?tattaccatg?gtgatgcggt?tttggcagta?catcaatggg?cgtggatagc 2820
ggtttgactc?acggggattt?ccaagtctcc?accccattga?cgtcaatggg?agtttgtttt 2880
ggcaccaaaa?tcaacgggac?tttccaaaat?gtcgtaacaa?ctccgcccca?ttgacgcaaa 2940
tgggcggtag?gcgtgtacgg?tgggaggtct?atataagcag?agctcaagct?tgctgcttgc 3000
acttcagagt?tctaggagag?tccctcctag?tctctcctct?ccgaggaggt?accgagacct 3060
caaaataaag?gagtgattgc?cttactgccg?agtggagagt?gattactgag?cggccggtgt 3120
atcgggagtc?gtcccttaat?ctgtgcaata?ccagagcggc?tctcgcagct?ggcgcccaac 3180
gtggggcccg?aggagaagaa?aagaaagcgg?ccctgagaac?tcggcttctg?aaaaagagga 3240
agaggacaag?ttgctatagc?aacaagagag?aagaagtaga?gcaaaggtcc?agtggctcgg 3300
aaaaagagga?actgaaactt?cggggacgcc?tgaaggagta?aggtaagtga?ctctgctgta 3360
cgcggggcga?ggcagaggtt?tccttctaaa?ttgaaagaga?agtgttgctg?cgagaggtct 3420
tggtggtcga?gaatcctgta?caaaaaaaag?gagggatctc?ggtcaggacc?aggacccctg 3480
ggagtaatac?aacagcaaca?ccgtaagaaa?atccgcctag?gtgagtctag?atagagacta 3540
ggcgaggcaa?gtctccgggg?ggaaaagaga?ttatcctgag?ctcgaaaaat?gtatcaagca 3600
tgcatgcaag?ataaaagttc?gactcagagg?ggagcacttg?acagaaggaa?attgtttatg 3660
gtgccttaaa?acattagatt?acatgtttga?ggaccataaa?gaggaacctt?ggacaaaagt 3720
aaaatttagg?acaatatggc?agaaggtgaa?gaatctaact?cctgaggaga?gtaacaaaaa 3780
agactttatg?tctttgcagg?ccacattagc?gggtctaatg?tgttgccaaa?tggggatgag 3840
acctgcagga?attcgattct?agaggtgata?gaaatgccag?aaaactatgc?aaaaacaaga 3900
atcataaaca?ggaaaaaaag?agaactcagc?cacaagagga?agaagagagg?cgttggcttg 3960
gtcattatgc?tagttatcat?ggcaatagta?gctgccgcag?gggcttctct?gggagtcgca 4020
aacgcgattc?agcagtctta?cactaaggca?gctgtccaga?cccttgctaa?tgcaactgct 4080
gcacagcagg?atgtgttaga?ggcaacctat?gccatggtac?agcatgtggc?taaaggcgta 4140
cgaatcttgg?aagctcgagt?ggctcgagtg?gaagctatca?cagatagaat?aatgctatac 4200
caagaattgg?attgttggca?ctaggatcca?tcgccacc 4238
<210>69
<211>4523
<212>DNA
＜213〉artificial sequence
<220>
<223>pCAH/SINd2
<400>69
atccagcaca?gtggcggccg?ctagcacaaa?aataaaaaaa?gaaagggtgg?actgtgagac 60
atgggctaaa?gaggagcggc?cgctcgagtc?tagaactagt?ggatcagctt?tgctgcttgc 120
acttcagagt?tctaggagag?tccctcctag?tctctcctct?ccgaggaggt?accgagacct 180
caaaataaag?gagtgattgc?cttactgccg?agtggagagt?gattactgag?cggccggtgt 240
atcgggagtc?gtcccttaat?ctgtgcaata?ccagagcggc?tctcgcagcc?gacctcgagg 300
gggggcccta?ttctatagtg?tcacctaaat?gctagagctc?gctgatcagc?ctcgactgtg 360
ccttctagtt?gccagccatc?tgttgtttgc?ccctcccccg?tgccttcctt?gaccctggaa 420
ggtgccactc?ccactgtcct?ttcctaataa?aatgaggaaa?ttgcatcgca?ttgtctgagt 480
aggtgtcatt?ctattctggg?gggtggggtg?gggcaggaca?gcaaggggga?ggattgggaa 540
gacaatagca?ggcatgctgg?ggatgcggtg?ggctctatgg?cttctgaggc?ggaaagaacc 600
agtggcggta?atacggttat?ccacagaatc?aggggataac?gcaggaaaga?acatgtgagc 660
aaaaggccag?caaaaggcca?ggaaccgtaa?aaaggccgcg?ttgctggcgt?ttttccatag 720
gctccgcccc?cctgacgagc?atcacaaaaa?tcgacgctca?agtcagaggt?ggcgaaaccc 780
gacaggacta?taaagatacc?aggcgtttcc?ccctggaagc?tccctcgtgc?gctctcctgt 840
tccgaccctg?ccgcttaccg?gatacctgtc?cgcctttctc?ccttcgggaa?gcgtggcgct 900
ttctcatagc?tcacgctgta?ggtatctcag?ttcggtgtag?gtcgttcgct?ccaagctggg 960
ctgtgtgcac?gaaccccccg?ttcagcccga?ccgctgcgcc?ttatccggta?actatcgtct 1020
tgagtccaac?ccggtaagac?acgacttatc?gccactggca?gcagccactg?gtaacaggat 1080
tagcagagcg?aggtatgtag?gcggtgctac?agagttcttg?aagtggtggc?ctaactacgg 1140
ctacactaga?aggacagtat?ttggtatctg?cgctctgctg?aagccagtta?ccttcggaaa 1200
aagagttggt?agctcttgat?ccggcaaaca?aaccaccgct?ggtagcggtg?gtttttttgt 1260
ttgcaagcag?cagattacgc?gcagaaaaaa?aggatctcaa?gaagatcctt?tgatcttttc 1320
tacggggtct?gacgctcagt?ggaacgaaaa?ctcacgttaa?gggattttgg?tcatgagatt 1380
atcaaaaagg?atcttcacct?agatcctttt?aaattaaaaa?tgaagtttta?aatcaatcta 1440
aagtatatat?gagtaacctg?atcaggactc?ttccttttca?tgaacaataa?aactgtctgc 1500
ttacataaac?agtaatacaa?ggggtgttat?gagccatatt?caacgggaaa?cgtcttgctc 1560
taggccgcga?ttaaattcca?acatggatgc?tgatttatat?gggtataaat?gggctcgcga 1620
taatgtcggg?caatcaggtg?cgacaatcta?tcgattgtat?gggaagcccg?atgcgccaga 1680
gttgtttctg?aaacatggca?aaggtagcgt?tgccaatgat?gttacagatg?agatggtcag 1740
actaaactgg?ctgacggaat?ttatgcctct?tccgaccatc?aagcatttta?tccgtactcc 1800
tgatgatgca?tggttactca?ccactgcgat?ccccgggaaa?acagcattcc?aggtattaga 1860
agaatatcct?gattcaggtg?aaaatattgt?tgatgcgctg?gcagtgttcc?tgcgccggtt 1920
gcattcgatt?cctgtttgta?attgtccttt?taacagcgat?cgcgtatttc?gtctcgctca 1980
ggcgcaatca?cgaatgaata?acggtttggt?tgatgcgagt?gattttgatg?acgagcgtaa 2040
tggctggcct?gttgaacaag?tctggaaaga?aatgcataaa?cttttgccat?tctcaccgga 2100
ttcagtcgtc?actcatggtg?atttctcact?tgataacctt?atttttgacg?aggggaaatt 2160
aataggttgt?attgatgttg?gacgagtcgg?aatcgcagac?cgataccagg?atcttgccat 2220
cctatggaac?tgcctcggtg?agttttctcc?ttcattacag?aaacggcttt?ttcaaaaata 2280
tggtattgat?aatcctgata?tgaataaatt?gcagtttcat?ttgatgctcg?atgagttttt 2340
ctaagaattc?gcgcaattaa?ccctcactaa?agggaacaaa?agctgggtac?cgggcccgtt 2400
gacattgatt?attgactagt?tattaatagt?aatcaattac?ggggtcatta?gttcatagcc 2460
catatatgga?gttccgcgtt?acataactta?cggtaaatgg?cccgcctggc?tgaccgccca 2520
acgacccccg?cccattgacg?tcaataatga?cgtatgttcc?catagtaacg?ccaataggga 2580
ctttccattg?acgtcaatgg?gtggagtatt?tacggtaaac?tgcccacttg?gcagtacatc 2640
aagtgtatca?tatgccaagt?acgcccccta?ttgacgtcaa?tgacggtaaa?tggcccgcct 2700
ggcattatgc?ccagtacatg?accttatggg?actttcctac?ttggcagtac?atctacgtat 2760
tagtcatcgc?tattaccatg?gtgatgcggt?tttggcagta?catcaatggg?cgtggatagc 2820
ggtttgactc?acggggattt?ccaagtctcc?accccattga?cgtcaatggg?agtttgtttt 2880
ggcaccaaaa?tcaacgggac?tttccaaaat?gtcgtaacaa?ctccgcccca?ttgacgcaaa 2940
tgggcggtag?gcgtgtacgg?tgggaggtct?atataagcag?agctcaagct?tgctgcttgc 3000
acttcagagt?tctaggagag?tccctcctag?tctctcctct?ccgaggaggt?accgagacct 3060
caaaataaag?gagtgattgc?cttactgccg?agtggagagt?gattactgag?cggccggtgt 3120
atcgggagtc?gtcccttaat?ctgtgcaata?ccagagcggc?tctcgcagct?ggcgcccaac 3180
gtggggcccg?aggagaagaa?aagaaagcgg?ccctgagaac?tcggcttctg?aaaaagagga 3240
agaggacaag?ttgctatagc?aacaagagag?aagaagtaga?gcaaaggtcc?agtggctcgg 3300
aaaaagagga?actgaaactt?cggggacgcc?tgaaggagta?aggtaagtga?ctctgctgta 3360
cgcggggcga?ggcagaggtt?tccttctaaa?ttgaaagaga?agtgttgctg?cgagaggtct 3420
tggtggtcga?gaatcctgta?caaaaaaaag?gagggatctc?ggtcaggacc?aggacccctg 3480
ggagtaatac?aacagcaaca?ccgtaagaaa?atccgcctag?gtgagtctag?atagagacta 3540
ggcgaggcaa?gtctccgggg?ggaaaagaga?ttatcctgag?ctcgaaaaat?gtatcaagca 3600
tgcatgcaag?ataaaagttc?gactcagagg?ggagcacttg?acagaaggaa?attgtttatg 3660
gtgccttaaa?acattagatt?acatgtttga?ggaccataaa?gaggaacctt?ggacaaaagt 3720
aaaatttagg?acaatatggc?agaaggtgaa?gaatctaact?cctgaggaga?gtaacaaaaa 3780
agactttatg?tctttgcagg?ccacattagc?gggtctaatg?tgttgccaaa?tggggatgag 3840
acctgagaca?ttgcaagatg?caatggctac?agtaatcatg?aaagatgggt?tactggaaca 3900
agaggaaaag?aaggaagaca?aaagagaaaa?ggaagagagt?gtcttcccaa?tagtagtgca 3960
agcagcagga?gggagaagct?ggaaagcagt?agattctgta?atgttccagc?aactgcaaac 4020
agtagcaatg?cagcatggcc?tcgtgtctga?ggactttgaa?aggcagttgg?catattatgc 4080
tactacctgg?acaagtaaag?acatactaga?agtattggcc?atgatgcctg?caggaattcg 4140
attctagagg?tgatagaaat?gccagaaaac?tatgcaaaaa?caagaatcat?aaacaggaaa 4200
aaaagagaac?tcagccacaa?gaggaagaag?agaggcgttg?gcttggtcat?tatgctagtt 4260
atcatggcaa?tagtagctgc?cgcaggggct?tctctgggag?tcgcaaacgc?gattcagcag 4320
tcttacacta?aggcagctgt?ccagaccctt?gctaatgcaa?ctgctgcaca?gcaggatgtg 4380
ttagaggcaa?cctatgccat?ggtacagcat?gtggctaaag?gcgtacgaat?cttggaagct 4440
cgagtggctc?gagtggaagc?tatcacagat?agaataatgc?tataccaaga?attggattgt 4500
tggcactagg?atccatcgcc?acc 4523
<210>70
<211>4819
<212>DNA
＜213〉artificial sequence
<220>
<223>pCAH/SINd3
<400>70
atccagcaca?gtggcggccg?ctagcacaaa?aataaaaaaa?gaaagggtgg?actgtgagac 60
atgggctaaa?gaggagcggc?cgctcgagtc?tagaactagt?ggatcagctt?tgctgcttgc 120
acttcagagt?tctaggagag?tccctcctag?tctctcctct?ccgaggaggt?accgagacct 180
caaaataaag?gagtgattgc?cttactgccg?agtggagagt?gattactgag?cggccggtgt 240
atcgggagtc?gtcccttaat?ctgtgcaata?ccagagcggc?tctcgcagcc?gacctcgagg 300
gggggcccta?ttctatagtg?tcacctaaat?gctagagctc?gctgatcagc?ctcgactgtg 360
ccttctagtt?gccagccatc?tgttgtttgc?ccctcccccg?tgccttcctt?gaccctggaa 420
ggtgccactc?ccactgtcct?ttcctaataa?aatgaggaaa?ttgcatcgca?ttgtctgagt 480
aggtgtcatt?ctattctggg?gggtggggtg?gggcaggaca?gcaaggggga?ggattgggaa 540
gacaatagca?ggcatgctgg?ggatgcggtg?ggctctatgg?cttctgaggc?ggaaagaacc 600
agtggcggta?atacggttat?ccacagaatc?aggggataac?gcaggaaaga?acatgtgagc 660
aaaaggccag?caaaaggcca?ggaaccgtaa?aaaggccgcg?ttgctggcgt?ttttccatag 720
gctccgcccc?cctgacgagc?atcacaaaaa?tcgacgctca?agtcagaggt?ggcgaaaccc 780
gacaggacta?taaagatacc?aggcgtttcc?ccctggaagc?tccctcgtgc?gctctcctgt 840
tccgaccctg?ccgcttaccg?gatacctgtc?cgcctttctc?ccttcgggaa?gcgtggcgct 900
ttctcatagc?tcacgctgta?ggtatctcag?ttcggtgtag?gtcgttcgct?ccaagctggg 960
ctgtgtgcac?gaaccccccg?ttcagcccga?ccgctgcgcc?ttatccggta?actatcgtct 1020
tgagtccaac?ccggtaagac?acgacttatc?gccactggca?gcagccactg?gtaacaggat 1080
tagcagagcg?aggtatgtag?gcggtgctac?agagttcttg?aagtggtggc?ctaactacgg 1140
ctacactaga?aggacagtat?ttggtatctg?cgctctgctg?aagccagtta?ccttcggaaa 1200
aagagttggt?agctcttgat?ccggcaaaca?aaccaccgct?ggtagcggtg?gtttttttgt 1260
ttgcaagcag?cagattacgc?gcagaaaaaa?aggatctcaa?gaagatcctt?tgatcttttc 1320
tacggggtct?gacgctcagt?ggaacgaaaa?ctcacgttaa?gggattttgg?tcatgagatt 1380
atcaaaaagg?atcttcacct?agatcctttt?aaattaaaaa?tgaagtttta?aatcaatcta 1440
aagtatatat?gagtaacctg?atcaggactc?ttccttttca?tgaacaataa?aactgtctgc 1500
ttacataaac?agtaatacaa?ggggtgttat?gagccatatt?caacgggaaa?cgtcttgctc 1560
taggccgcga?ttaaattcca?acatggatgc?tgatttatat?gggtataaat?gggctcgcga 1620
taatgtcggg?caatcaggtg?cgacaatcta?tcgattgtat?gggaagcccg?atgcgccaga 1680
gttgtttctg?aaacatggca?aaggtagcgt?tgccaatgat?gttacagatg?agatggtcag 1740
actaaactgg?ctgacggaat?ttatgcctct?tccgaccatc?aagcatttta?tccgtactcc 1800
tgatgatgca?tggttactca?ccactgcgat?ccccgggaaa?acagcattcc?aggtattaga 1860
agaatatcct?gattcaggtg?aaaatattgt?tgatgcgctg?gcagtgttcc?tgcgccggtt 1920
gcattcgatt?cctgtttgta?attgtccttt?taacagcgat?cgcgtatttc?gtctcgctca 1980
ggcgcaatca?cgaatgaata?acggtttggt?tgatgcgagt?gattttgatg?acgagcgtaa 2040
tggctggcct?gttgaacaag?tctggaaaga?aatgcataaa?cttttgccat?tctcaccgga 2100
ttcagtcgtc?actcatggtg?atttctcact?tgataacctt?atttttgacg?aggggaaatt 2160
aataggttgt?attgatgttg?gacgagtcgg?aatcgcagac?cgataccagg?atcttgccat 2220
cctatggaac?tgcctcggtg?agttttctcc?ttcattacag?aaacggcttt?ttcaaaaata 2280
tggtattgat?aatcctgata?tgaataaatt?gcagtttcat?ttgatgctcg?atgagttttt 2340
ctaagaattc?gcgcaattaa?ccctcactaa?agggaacaaa?agctgggtac?cgggcccgtt 2400
gacattgatt?attgactagt?tattaatagt?aatcaattac?ggggtcatta?gttcatagcc 2460
catatatgga?gttccgcgtt?acataactta?cggtaaatgg?cccgcctggc?tgaccgccca 2520
acgacccccg?cccattgacg?tcaataatga?cgtatgttcc?catagtaacg?ccaataggga 2580
ctttccattg?acgtcaatgg?gtggagtatt?tacggtaaac?tgcccacttg?gcagtacatc 2640
aagtgtatca?tatgccaagt?acgcccccta?ttgacgtcaa?tgacggtaaa?tggcccgcct 2700
ggcattatgc?ccagtacatg?accttatggg?actttcctac?ttggcagtac?atctacgtat 2760
tagtcatcgc?tattaccatg?gtgatgcggt?tttggcagta?catcaatggg?cgtggatagc 2820
ggtttgactc?acggggattt?ccaagtctcc?accccattga?cgtcaatggg?agtttgtttt 2880
ggcaccaaaa?tcaacgggac?tttccaaaat?gtcgtaacaa?ctccgcccca?ttgacgcaaa 2940
tgggcggtag?gcgtgtacgg?tgggaggtct?atataagcag?agctcaagct?tgctgcttgc 3000
acttcagagt?tctaggagag?tccctcctag?tctctcctct?ccgaggaggt?accgagacct 3060
caaaataaag?gagtgattgc?cttactgccg?agtggagagt?gattactgag?cggccggtgt 3120
atcgggagtc?gtcccttaat?ctgtgcaata?ccagagcggc?tctcgcagct?ggcgcccaac 3180
gtggggcccg?aggagaagaa?aagaaagcgg?ccctgagaac?tcggcttctg?aaaaagagga 3240
agaggacaag?ttgctatagc?aacaagagag?aagaagtaga?gcaaaggtcc?agtggctcgg 3300
aaaaagagga?actgaaactt?cggggacgcc?tgaaggagta?aggtaagtga?ctctgctgta 3360
cgcggggcga?ggcagaggtt?tccttctaaa?ttgaaagaga?agtgttgctg?cgagaggtct 3420
tggtggtcga?gaatcctgta?caaaaaaaag?gagggatctc?ggtcaggacc?aggacccctg 3480
ggagtaatac?aacagcaaca?ccgtaagaaa?atccgcctag?gtgagtctag?atagagacta 3540
ggcgaggcaa?gtctccgggg?ggaaaagaga?ttatcctgag?ctcgaaaaat?gtatcaagca 3600
tgcatgcaag?ataaaagttc?gactcagagg?ggagcacttg?acagaaggaa?attgtttatg 3660
gtgccttaaa?acattagatt?acatgtttga?ggaccataaa?gaggaacctt?ggacaaaagt 3720
aaaatttagg?acaatatggc?agaaggtgaa?gaatctaact?cctgaggaga?gtaacaaaaa 3780
agactttatg?tctttgcagg?ccacattagc?gggtctaatg?tgttgccaaa?tggggatgag 3840
acctgagaca?ttgcaagatg?caatggctac?agtaatcatg?aaagatgggt?tactggaaca 3900
agaggaaaag?aaggaagaca?aaagagaaaa?ggaagagagt?gtcttcccaa?tagtagtgca 3960
agcagcagga?gggagaagct?ggaaagcagt?agattctgta?atgttccagc?aactgcaaac 4020
agtagcaatg?cagcatggcc?tcgtgtctga?ggactttgaa?aggcagttgg?catattatgc 4080
tactacctgg?acaagtaaag?acatactaga?agtattggcc?atgatgcctg?gaaatagagc 4140
tcaaaaggag?ttaattcaag?ggaaattaaa?tgaagaagca?gaaaggtgga?gaaggaataa 4200
tccaccacct?ccagcaggag?gaggattaac?agtggatcaa?attatggggg?taggacaaac 4260
aaatcaagca?gcagcacaag?ctaacatgga?tcaggcaagg?caaatatgcc?tgcaatgggt 4320
aataaatgca?ttaagagcag?taagacatat?ggcgcacagg?ccagggaatc?caatgctagt?4380
aaagcaaaaa?acgaatgagc?catatgaaga?ttttgcagca?agactgcagg?aattcgattc?4440
tagaggtgat?agaaatgcca?gaaaactatg?caaaaacaag?aatcataaac?aggaaaaaaa?4500
gagaactcag?ccacaagagg?aagaagagag?gcgttggctt?ggtcattatg?ctagttatca?4560
tggcaatagt?agctgccgca?ggggcttctc?tgggagtcgc?aaacgcgatt?cagcagtctt?4620
acactaaggc?agctgtccag?acccttgcta?atgcaactgc?tgcacagcag?gatgtgttag?4680
aggcaaccta?tgccatggta?cagcatgtgg?ctaaaggcgt?acgaatcttg?gaagctcgag?4740
tggctcgagt?ggaagctatc?acagatagaa?taatgctata?ccaagaattg?gattgttggc?4800
actaggatcc?atcgccacc 4819
<210>71
<211>5112
<212>DNA
＜213〉artificial sequence
<220>
<223>pCAH/SINd4
<400>71
atccagcaca?gtggcggccg?ctagcacaaa?aataaaaaaa?gaaagggtgg?actgtgagac 60
atgggctaaa?gaggagcggc?cgctcgagtc?tagaactagt?ggatcagctt?tgctgcttgc 120
acttcagagt?tctaggagag?tccctcctag?tctctcctct?ccgaggaggt?accgagacct 180
caaaataaag?gagtgattgc?cttactgccg?agtggagagt?gattactgag?cggccggtgt 240
atcgggagtc?gtcccttaat?ctgtgcaata?ccagagcggc?tctcgcagcc?gacctcgagg 300
gggggcccta?ttctatagtg?tcacctaaat?gctagagctc?gctgatcagc?ctcgactgtg 360
ccttctagtt?gccagccatc?tgttgtttgc?ccctcccccg?tgccttcctt?gaccctggaa 420
ggtgccactc?ccactgtcct?ttcctaataa?aatgaggaaa?ttgcatcgca?ttgtctgagt 480
aggtgtcatt?ctattctggg?gggtggggtg?gggcaggaca?gcaaggggga?ggattgggaa 540
gacaatagca?ggcatgctgg?ggatgcggtg?ggctctatgg?cttctgaggc?ggaaagaacc 600
agtggcggta?atacggttat?ccacagaatc?aggggataac?gcaggaaaga?acatgtgagc 660
aaaaggccag?caaaaggcca?ggaaccgtaa?aaaggccgcg?ttgctggcgt?ttttccatag 720
gctccgcccc?cctgacgagc?atcacaaaaa?tcgacgctca?agtcagaggt?ggcgaaaccc 780
gacaggacta?taaagatacc?aggcgtttcc?ccctggaagc?tccctcgtgc?gctctcctgt 840
tccgaccctg?ccgcttaccg?gatacctgtc?cgcctttctc?ccttcgggaa?gcgtggcgct 900
ttctcatagc?tcacgctgta?ggtatctcag?ttcggtgtag?gtcgttcgct?ccaagctggg 960
ctgtgtgcac?gaaccccccg?ttcagcccga?ccgctgcgcc?ttatccggta?actatcgtct 1020
tgagtccaac?ccggtaagac?acgacttatc?gccactggca?gcagccactg?gtaacaggat 1080
tagcagagcg?aggtatgtag?gcggtgctac?agagttcttg?aagtggtggc?ctaactacgg 1140
ctacactaga?aggacagtat?ttggtatctg?cgctctgctg?aagccagtta?ccttcggaaa 1200
aagagttggt?agctcttgat?ccggcaaaca?aaccaccgct?ggtagcggtg?gtttttttgt 1260
ttgcaagcag?cagattacgc?gcagaaaaaa?aggatctcaa?gaagatcctt?tgatcttttc 1320
tacggggtct?gacgctcagt?ggaacgaaaa?ctcacgttaa?gggattttgg?tcatgagatt 1380
atcaaaaagg?atcttcacct?agatcctttt?aaattaaaaa?tgaagtttta?aatcaatcta 1440
aagtatatat?gagtaacctg?atcaggactc?ttccttttca?tgaacaataa?aactgtctgc 1500
ttacataaac?agtaatacaa?ggggtgttat?gagccatatt?caacgggaaa?cgtcttgctc 1560
taggccgcga?ttaaattcca?acatggatgc?tgatttatat?gggtataaat?gggctcgcga 1620
taatgtcggg?caatcaggtg?cgacaatcta?tcgattgtat?gggaagcccg?atgcgccaga 1680
gttgtttctg?aaacatggca?aaggtagcgt?tgccaatgat?gttacagatg?agatggtcag 1740
actaaactgg?ctgacggaat?ttatgcctct?tccgaccatc?aagcatttta?tccgtactcc 1800
tgatgatgca?tggttactca?ccactgcgat?ccccgggaaa?acagcattcc?aggtattaga 1860
agaatatcct?gattcaggtg?aaaatattgt?tgatgcgctg?gcagtgttcc?tgcgccggtt 1920
gcattcgatt?cctgtttgta?attgtccttt?taacagcgat?cgcgtatttc?gtctcgctca 1980
ggcgcaatca?cgaatgaata?acggtttggt?tgatgcgagt?gattttgatg?acgagcgtaa 2040
tggctggcct?gttgaacaag?tctggaaaga?aatgcataaa?cttttgccat?tctcaccgga 2100
ttcagtcgtc?actcatggtg?atttctcact?tgataacctt?atttttgacg?aggggaaatt 2160
aataggttgt?attgatgttg?gacgagtcgg?aatcgcagac?cgataccagg?atcttgccat 2220
cctatggaac?tgcctcggtg?agttttctcc?ttcattacag?aaacggcttt?ttcaaaaata 2280
tggtattgat?aatcctgata?tgaataaatt?gcagtttcat?ttgatgctcg?atgagttttt 2340
ctaagaattc?gcgcaattaa?ccctcactaa?agggaacaaa?agctgggtac?cgggcccgtt 2400
gacattgatt?attgactagt?tattaatagt?aatcaattac?ggggtcatta?gttcatagcc 2460
catatatgga?gttccgcgtt?acataactta?cggtaaatgg?cccgcctggc?tgaccgccca 2520
acgacccccg?cccattgacg?tcaataatga?cgtatgttcc?catagtaacg?ccaataggga 2580
ctttccattg?acgtcaatgg?gtggagtatt?tacggtaaac?tgcccacttg?gcagtacatc 2640
aagtgtatca?tatgccaagt?acgcccccta?ttgacgtcaa?tgacggtaaa?tggcccgcct 2700
ggcattatgc?ccagtacatg?accttatggg?actttcctac?ttggcagtac?atctacgtat 2760
tagtcatcgc?tattaccatg?gtgatgcggt?tttggcagta?catcaatggg?cgtggatagc 2820
ggtttgactc?acggggattt?ccaagtctcc?accccattga?cgtcaatggg?agtttgtttt 2880
ggcaccaaaa?tcaacgggac?tttccaaaat?gtcgtaacaa?ctccgcccca?ttgacgcaaa 2940
tgggcggtag?gcgtgtacgg?tgggaggtct?atataagcag?agctcaagct?tgctgcttgc 3000
acttcagagt?tctaggagag?tccctcctag?tctctcctct?ccgaggaggt?accgagacct 3060
caaaataaag?gagtgattgc?cttactgccg?agtggagagt?gattactgag?cggccggtgt 3120
atcgggagtc?gtcccttaat?ctgtgcaata?ccagagcggc?tctcgcagct?ggcgcccaac 3180
gtggggcccg?aggagaagaa?aagaaagcgg?ccctgagaac?tcggcttctg?aaaaagagga 3240
agaggacaag?ttgctatagc?aacaagagag?aagaagtaga?gcaaaggtcc?agtggctcgg 3300
aaaaagagga?actgaaactt?cggggacgcc?tgaaggagta?aggtaagtga?ctctgctgta 3360
cgcggggcga?ggcagaggtt?tccttctaaa?ttgaaagaga?agtgttgctg?cgagaggtct 3420
tggtggtcga?gaatcctgta?caaaaaaaag?gagggatctc?ggtcaggacc?aggacccctg 3480
ggagtaatac?aacagcaaca?ccgtaagaaa?atccgcctag?gtgagtctag?atagagacta 3540
ggcgaggcaa?gtctccgggg?ggaaaagaga?ttatcctgag?ctcgaaaaat?gtatcaagca 3600
tgcatgcaag?ataaaagttc?gactcagagg?ggagcacttg?acagaaggaa?attgtttatg 3660
gtgccttaaa?acattagatt?acatgtttga?ggaccataaa?gaggaacctt?ggacaaaagt 3720
aaaatttagg?acaatatggc?agaaggtgaa?gaatctaact?cctgaggaga?gtaacaaaaa 3780
agactttatg?tctttgcagg?ccacattagc?gggtctaatg?tgttgccaaa?tggggatgag 3840
acctgagaca?ttgcaagatg?caatggctac?agtaatcatg?aaagatgggt?tactggaaca 3900
agaggaaaag?aaggaagaca?aaagagaaaa?ggaagagagt?gtcttcccaa?tagtagtgca 3960
agcagcagga?gggagaagct?ggaaagcagt?agattctgta?atgttccagc?aactgcaaac 4020
agtagcaatg?cagcatggcc?tcgtgtctga?ggactttgaa?aggcagttgg?catattatgc 4080
tactacctgg?acaagtaaag?acatactaga?agtattggcc?atgatgcctg?gaaatagagc 4140
tcaaaaggag?ttaattcaag?ggaaattaaa?tgaagaagca?gaaaggtgga?gaaggaataa 4200
tccaccacct?ccagcaggag?gaggattaac?agtggatcaa?attatggggg?taggacaaac 4260
aaatcaagca?gcagcacaag?ctaacatgga?tcaggcaagg?caaatatgcc?tgcaatgggt 4320
aataaatgca?ttaagagcag?taagacatat?ggcgcacagg?ccagggaatc?caatgctagt 4380
aaagcaaaaa?acgaatgagc?catatgaaga?ttttgcagca?agactgctag?aagcaataga 4440
tgcagagcca?gttacacagc?ctataaaaga?ttatctaaag?ctaacactat?cttatacaaa 4500
tgcatcagca?gattgtcaga?agcaaatgga?tagaacacta?ggacaaagag?tacaacaagc 4560
tagtgtagaa?gaaaaaatgc?aagcatgtag?agatgtggga?tcagaagggt?tcaaaatgca 4620
attgttagca?caagcattaa?ggccaggaaa?aggaaaaggg?aatggacagc?cacaaaggtg 4680
ttacaactgt?ggaaaaccgg?gacatcaagc?aaggcactgc?aggaattcga?ttctagaggt 4740
gatagaaatg?ccagaaaact?atgcaaaaac?aagaatcata?aacaggaaaa?aaagagaact 4800
cagccacaag?aggaagaaga?gaggcgttgg?cttggtcatt?atgctagtta?tcatggcaat 4860
agtagctgcc?gcaggggctt?ctctgggagt?cgcaaacgcg?attcagcagt?cttacactaa 4920
ggcagctgtc?cagacccttg?ctaatgcaac?tgctgcacag?caggatgtgt?tagaggcaac 4980
ctatgccatg?gtacagcatg?tggctaaagg?cgtacgaatc?ttggaagctc?gagtggctcg 5040
agtggaagct?atcacagata?gaataatgct?ataccaagaa?ttggattgtt?ggcactagga 5100
tccatcgcca?cc 5112
<210>72
<211>7579
<212>DNA
＜213〉artificial sequence
<220>
<223>pMYKEF1/env
<220>
<221>misc_feature
<222>(994)
<223>n?is?a，c，g，or?t
<400>72
aacaggaaag?ttccattgga?gccaagtaca?ttgagtcaat?agggactttc?caatgggttt 60
tgcccagtac?ataaggtcaa?tgggaggtaa?gccaatgggt?ttttcccatt?actggcacgt 120
atactgagtc?attagggact?ttccaatggg?ttttgcccag?tacataaggt?caataggggt 180
gaatcaacag?gaaagtccca?ttggagccaa?gtacactgag?tcaataggga?ctttccattg 240
ggttttgccc?agtacaaaag?gtcaataggg?ggtgagtcaa?tgggtttttc?ccattattgg 300
cacgtacata?aggtcaatag?gggtgagtca?ttgggttttt?ccagccaatt?taattaaaac 360
gccatgtact?ttcccaccat?tgacgtcaat?gggctattga?aactaatgca?acgtgacctt 420
taaacggtac?tttcccatag?ctgattaatg?ggaaagtacc?gttctcgagc?caatacacgt 480
caatgggaag?tgaaagggca?gccaaaacgt?aacaccgccc?cggttttccc?tggaaattcc 540
atattggcac?gcattctatt?ggctgagctg?cgttcacgtg?ggtataagag?gcgcgaccag 600
cgtcggtacc?gtcgcagtct?tcggtctgac?caccgtagaa?cgcagagctc?ctcgctgcag 660
gcatgcaagc?ttggtaagtg?ccgtgtgtgg?ttcccgcggg?cctggcctct?ttacgggtta 720
tggcccttgc?gtgccttgaa?ttacttccac?gcccctggct?gcagtacgtg?attcttgatc 780
ccgagcttcg?ggttggaagt?gggtgggaga?gttcaaggcc?ttgcgcttaa?ggagcccctt 840
cgccttttgc?ttgagttgag?gcctggcctg?ggcgctgggg?ccgccgcgtg?caaatctggt 900
ggcaccttcg?cgcctgtctc?gctgctttcg?ataagtctct?agccatttaa?aatttttgat 960
gacctgctgc?gacgcttttt?ttctggcaag?atantcttgt?aaatgcgggc?caagatctgc 1020
acactggtat?ttcggttttt?ggggccgcgg?gcggctacgg?ggcccgtgcg?tcccagcgca 1080
catgttcggc?gaggaggggc?ctgcgagcgc?ggccaccgag?aatcggacgg?gggtagtctc 1140
aagctggccg?gcctgctctg?gtgcctggcc?tcgcgccgcc?gtgtatcgcc?ccgccctggg 1200
cggcaaggct?ggcccggtcg?gcaccagttg?cgtgagcgga?aagatggccg?cttcccggcc 1260
ctgctgcagg?gagctcaaaa?tggaggacgc?ggcgctcggg?agagcgggcg?ggtgagtcac 1320
ccacacaaag?gaaaagggcc?tttccgtcct?cagccgtcgc?ttcatgtgac?tccacggagt 1380
accgggcgcc?gtccaggcac?ctcgattagt?tctcgagctt?ttggagtacg?tcgtctttag 1440
gttgggggga?ggggttttat?gcgatggagt?ttccccacac?tgagtgggtg?gagactgaag 1500
ttaggccagc?ttggcacttg?atgtaattct?ccttggaatt?tgcccttttt?gagtttggat 1560
cttggttcat?tctcaagcct?cagacagtgg?ttcaaagttt?ttttcttcca?tttcagggat 1620
ccactagtaa?cggccgccag?tgtgctggaa?ttcgatcata?cctggtgttg?ctgactaccc 1680
cgaccgcggt?aaaagtcgat?ggtattgctg?cctgggtcca?tgcttctcac?ctcaaacctg 1740
caccaccttc?ggcaccagat?gagtcctggg?agctggaaaa?gactgatcat?cctcttaagc 1800
tgcgtattcg?gcggcggcgg?gacgagtctg?caaaataaga?acccccacca?gcccatgacc 1860
ctcacttggc?aggtactgtc?ccaaactgga?gacgttgtct?gggatacaaa?ggcagtccag 1920
cccccttgga?cttggtggcc?cacacttaaa?cctgatgtat?gtgccttggc?ggctagtctt 1980
gagtcctggg?atatcccggg?aaccgatgtc?tcgtcctcta?aacgagtcag?acctccggac 2040
tcagactata?ctgccgctta?taagcaaatc?acctggggag?ccatagggtg?cagctaccct 2100
cgggctagga?ctagaatggc?aagctctacc?ttctacgtat?gtccccggga?tggccggacc 2160
ctttcagaag?ctagaaggtg?cggggggcta?gaatccctat?actgtaaaga?atgggattgt 2220
gagaccacgg?ggaccggtta?ttggctatct?aaatcctcaa?aagacctcat?aactgtaaaa 2280
tgggaccaaa?atagcgaatg?gactcaaaaa?tttcaacagt?gtcaccagac?cggctggtgt 2340
aaccccctta?aaatagattt?cacagacaaa?ggaaaattat?ccaaggactg?gataacggga 2400
aaaacctggg?gattaagatt?ctatgtgtct?ggacatccag?gcgtacagtt?caccattcgc 2460
ttaaaaatca?ccaacatgcc?agctgtggca?gtaggtcctg?acctcgtcct?tgtggaacaa 2520
ggacctccta?gaacgtccct?cgctctccca?cctcctcttc?ccccaaggga?agcgccaccg 2580
ccatctctcc?ccgactctaa?ctccacagcc?ctggcgacta?gtgcacaaac?tcccacggtg 2640
agaaaaacaa?ttgttaccct?aaacactccg?cctcccacca?caggcgacag?actttttgat 2700
cttgtgcagg?gggccttcct?aaccttaaat?gctaccaacc?caggggccac?tgagtcttgc 2760
tggctttgtt?tggccatggg?ccccccttat?tatgaagcaa?tagcctcatc?aggagaggtc 2820
gcctactcca?ccgaccttga?ccggtgccgc?tgggggaccc?aaggaaagct?caccctcact 2880
gaggtctcag?gacacgggtt?gtgcatagga?aaggtgccct?ttacccatca?gcatctctgc 2940
aatcagaccc?tatccatcaa?ttcctccgga?gaccatcagt?atctgctccc?ctccaaccat 3000
agctggtggg?cttgcagcac?tggcctcacc?ccttgcctct?ccacctcagt?ttttaatcag 3060
actagagatt?tctgtatcca?ggtccagctg?attcctcgca?tctattacta?tcctgaagaa 3120
gttttgttac?aggcctatga?caattctcac?cccaggacta?aaagagaggc?tgtctcactt 3180
accctagctg?ttttactggg?gttgggaatc?acggcgggaa?taggtactgg?ttcaactgcc 3240
ttaattaaag?gacctataga?cctccagcaa?ggcctgacaa?gcctccagat?cgccatagat 3300
gctgacctcc?gggccctcca?agactcagtc?agcaagttag?aggactcact?gacttccctg 3360
tccgaggtag?tgctccaaaa?taggagaggc?cttgacttgc?tgtttctaaa?agaaggtggc 3420
ctctgtgcgg?ccctaaagga?agagtgctgt?ttttacatag?accactcagg?tgcagtacgg 3480
gactccatga?aaaaactcaa?agaaaaactg?gataaaagac?agttagagcg?ccagaaaagc 3540
caaaactggt?atgaaggatg?gttcaataac?tccccttggt?tcactaccct?gctatcaacc 3600
atcgctgggc?ccctattact?cctccttctg?ttgctcatcc?tcgggccatg?catcatcaat 3660
aagttagttc?aattcatcaa?tgataggata?agtgcagtta?aaattctggt?ccttagacaa 3720
aaatatcagg?ccctagagaa?cgaaggtaac?ctttaatttt?gctctaagat?tagagctatt 3780
cacaagagaa?atggggatca?ctagtgaatt?ctgcagatat?ccatcacact?ggcggccgct 3840
cgagcatgca?tctagagggc?cctattctat?agtgtcacct?aaatgctaga?gctcgctgat 3900
cagcctcgac?tgtgccttct?agttgccagc?catctgttgt?ttgcccctcc?cccgtgcctt 3950
ccttgaccct?ggaaggtgcc?actcccactg?tcctttccta?ataaaatgag?gaaattgcat 4020
cgcattgtct?gagtaggtgt?cattctattc?tggggggtgg?ggtggggcag?gacagcaagg 4080
gggaggattg?ggaagacaat?agcaggcatg?ctggggatgc?ggtgggctct?atggcttctg 4140
aggcggaaag?aaccagtggc?ggtaatacgg?ttatccacag?aatcagggga?taacgcagga 4200
aagaacatgt?gagcaaaagg?ccagcaaaag?gccaggaacc?gtaaaaaggc?cgcgttgctg 4260
gcgtttttcc?ataggctccg?cccccctgac?gagcatcaca?aaaatcgacg?ctcaagtcag 4320
aggtggcgaa?acccgacagg?actataaaga?taccaggcgt?ttccccctgg?aagctccctc 4380
gtgcgctctc?ctgttccgac?cctgccgctt?accggatacc?tgtccgcctt?tctcccttcg 4440
ggaagcgtgg?cgctttctca?tagctcacgc?tgtaggtatc?tcagttcggt?gtaggtcgtt 4500
cgctccaagc?tgggctgtgt?gcacgaaccc?cccgttcagc?ccgaccgctg?cgccttatcc 4560
ggtaactatc?gtcttgagtc?caacccggta?agacacgact?tatcgccact?ggcagcagcc 4620
actggtaaca?ggattagcag?agcgaggtat?gtaggcggtg?ctacagagtt?cttgaagtgg 4680
tggcctaact?acggctacac?tagaaggaca?gtatttggta?tctgcgctct?gctgaagcca 4740
gttaccttcg?gaaaaagagt?tggtagctct?tgatccggca?aacaaaccac?cgctggtagc 4800
ggtggttttt?ttgtttgcaa?gcagcagatt?acgcgcagaa?aaaaaggatc?tcaagaagat 4860
cctttgatct?tttctacggg?gtctgacgct?cagtggaacg?aaaactcacg?ttaagggatt 4920
ttggtcatga?gattatcaaa?aaggatcttc?acctagatcc?ttttaaatta?aaaatgaagt 4980
tttaaatcaa?tctaaagtat?atatgagtaa?cctgaggcta?tggcagggcc?tgccgccccg 5040
acgttggctg?cgagccctgg?gccttcaccc?gaacttgggg?ggtggggtgg?ggaaaaggaa 5100
gaaacgcggg?cgtattggcc?ccaatggggt?ctcggtgggg?tatcgacaga?gtgccagccc 5160
tgggaccgaa?ccccgcgttt?atgaacaaac?gacccaacac?cgtgcgtttt?attctgtctt 5220
tttattgccg?tcatagcgcg?ggttccttcc?ggtattgtct?ccttccgtgt?ttcagttagc 5280
ctccccctag?ggtgggcgaa?gaactccagc?atgagatccc?cgcgctggag?gatcatccag 5340
ccggcgtccc?ggaaaacgat?tccgaagccc?aacctttcat?agaaggcggc?ggtggaatcg 5400
aaatctcgtg?atggcaggtt?gggcgtcgct?tggtcggtca?tttcgaaccc?cagagtcccg 5460
ctcagaagaa?ctcgtcaaga?aggcgataga?aggcgatgcg?ctgcgaatcg?ggagcggcga 5520
taccgtaaag?cacgaggaag?cggtcagccc?attcgccgcc?aagctcttca?gcaatatcac 5580
gggtagccaa?cgctatgtcc?tgatagcggt?ccgccacacc?cagccggcca?cagtcgatga 5640
atccagaaaa?gcggccattt?tccaccatga?tattcggcaa?gcaggcatcg?ccatgggtca 5700
cgacgagatc?ctcgccgtcg?ggcatgctcg?ccttgagcct?ggcgaacagt?tcggctggcg 5760
cgagcccctg?atgctcttga?tcatcctgat?cgacaagacc?ggcttccatc?cgagtacgtg 5820
ctcgctcgat?gcgatgtttc?gcttggtggt?cgaatgggca?ggtagccgga?tcaagcgtat 5880
gcagccgccg?cattgcatca?gccatgatgg?atactttctc?ggcaggagca?aggtgagatg 5940
acaggagatc?ctgccccggc?acttcgccca?atagcagcca?gtcccttccc?gcttcagtga 6000
caacgtcgag?cacagctgcg?caaggaacgc?ccgtcgtggc?cagccacgat?agccgcgctg 6060
cctcgtcttg?cagttcattc?agggcaccgg?acaggtcggt?cttgacaaaa?agaaccgggc 6120
gcccctgcgc?tgacagccgg?aacacggcgg?catcagagca?gccgattgtc?tgttgtgccc 6180
agtcatagcc?gaatagcctc?tccacccaag?cggccggaga?acctgcgtgc?aatccatctt 5240
gttcaatcat?gcgaaacgat?cctcatcctg?tctcttgatc?gatctttgca?aaagcctagg 6300
cctccaaaaa?agcctcctca?ctacttctgg?aatagctcag?aggccgaggc?ggcctcggcc 6360
tctgcataaa?taaaaaaaat?tagtcagcca?tggggcggag?aatgggcgga?actgggcgga 6420
gttaggggcg?ggatgggcgg?agttaggggc?gggactatgg?ttgctgacta?attgagatgc 6480
atgctttgca?tacttctgcc?tgctggggag?cctggggact?ttccacacct?ggttgctgac 6540
taattgagat?gcatgctttg?catacttctg?cctgctgggg?agcctgggga?ctttccacac 6600
cctaactgac?acacattcca?cagctggttc?tttccgcctc?aggactcttc?ctttttcaat 6660
aaatcaatct?aaagtatata?tgagtaaact?tggtctgaca?gttaccaatg?cttaatcagt 6720
gaggcaccta?tctcagcgat?ctgtctattt?cgttcatcca?tagttgcctg?actccccgtc 6780
gtgtagataa?ctacgatacg?ggagggctta?ccatctggcc?ccagtgctgc?aatgataccg 6840
cgagacccac?gctcaccggc?tccagattta?tcagcaataa?accagccagc?cggaagggcc 6900
gagcgcagaa?gtggtcctgc?aactttatcc?gcctccatcc?agtctattaa?ttgttgccgg 6960
gaagctagag?taagtagttc?gccagttaat?agtttgcgca?acgttgttgc?cattgctaca 7020
ggcatcgtgg?tgtcacgctc?gtcgtttggt?atggcttcat?tcagctccgg?ttcccaacga 7080
tcaaggcgag?ttacatgatc?ccccatgttg?tgcaaaaaag?cggttagctc?cttcggtcct 7140
ccgatcgttg?tcagaagtaa?gttggccgca?gtgttatcac?tcatggttat?ggcagcactg 7200
cataattctc?ttactgtcat?gccatccgta?agatgctttt?ctgtgactgg?tgagtactca 7260
accaagtcat?tctgagaata?gtgtatgcgg?cgaccgagtt?gctcttgccc?ggcgtcaata 7320
cgggataata?ccgcgccaca?tagcagaact?ttaaaagtgc?tcatcattgg?aaaacgttct 7380
tcggggcgaa?aactctcaag?gatcttaccg?ctgttgagat?ccagttcgat?gtaacccact 7440
cgtgcaccca?actgatcttc?agcatctttt?actttcacca?gcgtttctgg?gtgagcaaaa 7500
acaggaaggc?aaaatgccgc?aaaaaaggga?ataagggcga?cacggaaatg?ttgaatactc 7560
atactcttcc?tttttcaat 7579
<210>73
<211>3566
<212>DNA
＜213〉artificial sequence
<220>
<223>pCAH/SINd
<400>73
atccagcaca?gtggcggccg?ctagcacaaa?aataaaaaaa?gaaagggtgg?actgtgagac 60
atgggctaaa?gaggagcggc?cgctcgagtc?tagaactagt?ggatcagctt?tgctgcttgc 120
acttcagagt?tctaggagag?tccctcctag?tctctcctct?ccgaggaggt?accgagacct 180
caaaataaag?gagtgattgc?cttactgccg?agtggagagt?gattactgag?cggccggtgt 240
atcgggagtc?gtcccttaat?ctgtgcaata?ccagagcggc?tctcgcagcc?gacctcgagg 300
gggggcccta?ttctatagtg?tcacctaaat?gctagagctc?gctgatcagc?ctcgactgtg 360
ccttctagtt?gccagccatc?tgttgtttgc?ccctcccccg?tgccttcctt?gaccctggaa 420
ggtgccactc?ccactgtcct?ttcctaataa?aatgaggaaa?ttgcatcgca?ttgtctgagt 480
aggtgtcatt?ctattctggg?gggtggggtg?gggcaggaca?gcaaggggga?ggattgggaa 540
gacaatagca?ggcatgctgg?ggatgcggtg?ggctctatgg?cttctgaggc?ggaaagaacc 600
agtggcggta?atacggttat?ccacagaatc?aggggataac?gcaggaaaga?acatgtgagc 660
aaaaggccag?caaaaggcca?ggaaccgtaa?aaaggccgcg?ttgctggcgt?ttttccatag 720
gctccgcccc?cctgacgagc?atcacaaaaa?tcgacgctca?agtcagaggt?ggcgaaaccc 780
gacaggacta?taaagatacc?aggcgtttcc?ccctggaagc?tccctcgtgc?gctctcctgt 840
tccgaccctg?ccgcttaccg?gatacctgtc?cgcctttctc?ccttcgggaa?gcgtggcgct 900
ttctcatagc?tcacgctgta?ggtatctcag?ttcggtgtag?gtcgttcgct?ccaagctggg 960
ctgtgtgcac?gaaccccccg?ttcagcccga?ccgctgcgcc?ttatccggta?actatcgtct 1020
tgagtccaac?ccggtaagac?acgacttatc?gccactggca?gcagccactg?gtaacaggat 1080
tagcagagcg?aggtatgtag?gcggtgctac?agagttcttg?aagtggtggc?ctaactacgg 1140
ctacactaga?aggacagtat?ttggtatctg?cgctctgctg?aagccagtta?ccttcggaaa 1200
aagagttggt?agctcttgat?ccggcaaaca?aaccaccgct?ggtagcggtg?gtttttttgt 1260
ttgcaagcag?cagattacgc?gcagaaaaaa?aggatctcaa?gaagatcctt?tgatcttttc 1320
tacggggtct?gacgctcagt?ggaacgaaaa?ctcacgttaa?gggattttgg?tcatgagatt 1380
atcaaaaagg?atcttcacct?agatcctttt?aaattaaaaa?tgaagtttta?aatcaatcta 1440
aagtatatat?gagtaacctg?atcaggactc?ttccttttca?tgaacaataa?aactgtctgc 1500
ttacataaac?agtaatacaa?ggggtgttat?gagccatatt?caacgggaaa?cgtcttgctc 1560
taggccgcga?ttaaattcca?acatggatgc?tgatttatat?gggtataaat?gggctcgcga 1620
taatgtcggg?caatcaggtg?cgacaatcta?tcgattgtat?gggaagcccg?atgcgccaga 1680
gttgtttctg?aaacatggca?aaggtagcgt?tgccaatgat?gttacagatg?agatggtcag 1740
actaaactgg?ctgacggaat?ttatgcctct?tccgaccatc?aagcatttta?tccgtactcc 1800
tgatgatgca?tggttactca?ccactgcgat?ccccgggaaa?acagcattcc?aggtattaga 1860
agaatatcct?gattcaggtg?aaaatattgt?tgatgcgctg?gcagtgttcc?tgcgccggtt 1920
gcattcgatt?cctgtttgta?attgtccttt?taacagcgat?cgcgtatttc?gtctcgctca 1980
ggcgcaatca?cgaatgaata?acggtttggt?tgatgcgagt?gattttgatg?acgagcgtaa 2040
tggctggcct?gttgaacaag?tctggaaaga?aatgcataaa?cttttgccat?tctcaccgga 2100
ttcagtcgtc?actcatggtg?atttctcact?tgataacctt?atttttgacg?aggggaaatt 2160
aataggttgt?attgatgttg?gacgagtcgg?aatcgcagac?cgataccagg?atcttgccat 2220
cctatggaac?tgcctcggtg?agttttctcc?ttcattacag?aaacggcttt?ttcaaaaata 2280
tggtattgat?aatcctgata?tgaataaatt?gcagtttcat?ttgatgctcg?atgagttttt 2340
ctaagaattc?gcgcaattaa?ccctcactaa?agggaacaaa?agctgggtac?cgggcccgtt 2400
gacattgatt?attgactagt?tattaatagt?aatcaattac?ggggtcatta?gttcatagcc 2460
catatatgga?gttccgcgtt?acataactta?cggtaaatgg?cccgcctggc?tgaccgccca 2520
acgacccccg?cccattgacg?tcaataatga?cgtatgttcc?catagtaacg?ccaataggga 2580
ctttccattg?acgtcaatgg?gtggagtatt?tacggtaaac?tgcccacttg?gcagtacatc 2640
aagtgtatca?tatgccaagt?acgcccccta?ttgacgtcaa?tgacggtaaa?tggcccgcct 2700
ggcattatgc?ccagtacatg?accttatggg?actttcctac?ttggcagtac?atctacgtat 2760
tagtcatcgc?tattaccatg?gtgatgcggt?tttggcagta?catcaatggg?cgtggatagc 2820
ggtttgactc?acggggattt?ccaagtctcc?accccattga?cgtcaatggg?agtttgtttt 2880
ggcaccaaaa?tcaacgggac?tttccaaaat?gtcgtaacaa?ctccgcccca?ttgacgcaaa 2940
tgggcggtag?gcgtgtacgg?tgggaggtct?atataagcag?agctcaagct?ttgctgcttg 3000
cacttcagag?ttctaggaga?gtccctccta?gtctctcctc?tccgaggagg?taccgagacc 3060
tcaaaataaa?ggagtgattg?ccttactgcc?gagtggagag?tgattactga?gcggccggtg 3120
tatcgggagt?cgtcccttaa?tctgtgcaat?accagagcgg?ctctcgcagc?tggcgggaat 3180
tcgattctag?aggtgataga?aatgccagaa?aactatgcaa?aaacaagaat?cataaacagg 3240
aaaaaaagag?aactcagcca?caagaggaag?aagagaggcg?ttggcttggt?cattatgcta 3300
gttatcatgg?caatagtagc?tgccgcaggg?gcttctctgg?gagtcgcaaa?cgcgattcag 3360
cagtcttaca?ctaaggcagc?tgtccagacc?cttgctaatg?caactgctgc?acagcaggat 3420
gtgttagagg?caacctatgc?catggtacag?catgtggcta?aaggcgtacg?aatcttggaa 3480
gctcgagtgg?ctcgagtgga?agctatcaca?gatagaataa?tgctatacca?agaattggat 3540
tgttggcact?aggatccatc?gccacc 3566
<210>74
<211>7623
<212>DNA
＜213〉artificial sequence
<220>
<223>pHGVSV-G
<400>74
gcgcgcgttg?acattgatta?ttgactagtt?attaatagta?atcaattacg?gggtcattag 60
ttcatagccc?atatatggag?ttccgcgtta?cataacttac?ggtaaatggc?ccgcctggct 120
gaccgcccaa?cgacccccgc?ccattgacgt?caataatgac?gtatgttccc?atagtaacgc 180
caatagggac?tttccattga?cgtcaatggg?tggactattt?acggtaaact?gcccacttgg 240
cagtacatca?agtgtatcat?atgccaagta?cgccccctat?tgacgtcaat?gacggtaaat 300
ggcccgcctg?gcattatgcc?cagtacatga?ccttatggga?ctttcctact?tggcagtaca 360
tctacgtatt?agtcatcgct?attaccatgg?tgatgcggtt?ttggcagtac?atcaatgggc 420
gtggatagcg?gtttgactca?cggggatttc?caagtctcca?ccccattgac?gtcaatggga 480
gtttgttttg?gcaccaaaat?caacgggact?ttccaaaatg?tcgtaacaac?tccgccccat 540
tgacgcaaat?gggcggtagg?cgtgtacggt?gggaggtcta?tataagcaga?gctctctggc 600
taactagaga?acccactgct?tactggctta?tcgaaattaa?tacgactcac?tatagggaga 660
cccaagcttg?gtaccgagct?cggatccact?agtaacggcc?gccagtgtgc?tggaattcga 720
tgatcctgag?aacttcaggg?tgagtctatg?ggacccttga?tgttttcttt?ccccttcttt 780
tctatggtta?agttcatgtc?ataggaaggg?gagaagtaac?agggtacagt?ttagaatggg 840
aaacagacga?atgattgcat?cagtgtggaa?gtctcaggat?cgttttagtt?tcttttattt 900
gctgttcata?acaattgttt?tcttttgttt?aattcttgct?ttcttttttt?ttcttctccg 960
caatttttac?tattatactt?aatgccttaa?cattgtgtat?aacaaaagga?aatatctctg 1020
agatacatta?agtaacttaa?aaaaaaactt?tacacagtct?gcctagtaca?ttactatttg 1080
gaatatatgt?gtgcttattt?gcatattcat?aatctcccta?ctttattttc?ttttattttt 1140
aattgataca?taatcattat?acatatttat?gggttaaagt?gtaatgtttt?aatatgtgta 1200
cacatattga?ccaaatcagg?gtaattttgc?atttgtaatt?ttaaaaaatg?ctttcttctt 1260
ttaatatact?tttttgttta?tcttatttct?aatactttcc?ctaatctctt?tctttcaggg 1320
caataatgat?acaatgtatc?atgcctcttt?gcaccattct?aaagaataac?agtgataatt 1380
tctgggttaa?ggcaatagca?atatttctgc?atataaatat?ttctgcatat?aaattgtaac 1440
tgatgtaaga?ggtttcatat?tgctaatagc?agctacaatc?cagctaccat?taggcccttt 1500
tgctaatcat?gttcatacct?cttatcttcc?tcccacagct?cctgggcaac?gtgctggtct 1560
gtgtgctggc?ccatcacttt?ggcaaagaat?cactagtgaa?ttctgcagat?atccatcaca 1620
ctggcggccg?ctcgaggaat?tctgacacta?tgaagtgcct?tttgtactta?gcctttttat 1680
tcattggggt?gaattgcaag?ttcaccatag?tttttccaca?caaccaaaaa?ggaaactgga 1740
aaaatgttcc?ttctaattac?cattattgcc?cgtcaagctc?agatttaaat?tggcataatg 1800
acttaatagg?cacagcctta?caagtcaaaa?tgcccaagag?tcacaaggct?attcaagcag 1860
acggttggat?gtgtcatgct?tccaaatggg?tcactacttg?tgatttccgc?tggtatggac 1920
cgaagtatat?aacacattcc?atccgatcct?tcactccatc?tgtagaacaa?tgcaaggaaa 1980
gcattgaaca?aacgaaacaa?ggaacttggc?tgaatccagg?cttccctcct?caaagttgtg 2040
gatatgcaac?tgtgacggat?gccgaagcag?tgattgtcca?ggtgactcct?caccatgtgc 2100
tggttgatga?atacacagga?gaatgggttg?attcacagtt?catcaacgga?aaatgcagca 2160
attacatatg?ccccactgtc?cataactcta?caacctggca?ttctgactat?aaggtcaaag 2220
ggctatgtga?ttctaacctc?atttccatgg?acatcacctt?cttctcagag?gacggagagc 2280
tatcatccct?gggaaaggag?ggcacagggt?tcagaagtaa?ctactttgct?tatgaaactg 2340
gaggcaaggc?ctgcaaaatg?caatactgca?agcattgggg?agtcagactc?ccatcaggtg 2400
tctggttcga?gatggctgat?aaggatctct?ttgctgcagc?cagattccct?gaatgcccag 2460
aagggtcaag?tatctctgct?ccatctcaga?cctcagtgga?tgtaagtcta?attcaggacg 2520
ttgagaggat?cttggattat?tccctctgcc?aagaaacctg?gagcaaaatc?agagcgggtc 2580
ttccaatctc?tccagtggat?ctcagctatc?ttgctcctaa?aaacccagga?accggtcctg 2640
ctttcaccat?aatcaatggt?accctaaaat?actttgagac?cagatacatc?agagtcgata 2700
ttgctgctcc?aatcctctca?agaatggtcg?gaatgatcag?tggaactacc?acagaaaggg 2760
aactgtggga?tgactgggca?ccatatgaag?acgtggaaat?tggacccaat?ggagttctga 2820
ggaccagttc?aggatataag?tttcctttat?acatgattgg?acatggtatg?ttggactccg 2880
atcttcatct?tagctcaaag?gctcaggtgt?tcgaacatcc?tcacattcaa?gacgctgctt 2940
cgcaacttcc?tgatgatgag?agtttatttt?ttggtgatac?tgggctatcc?aaaaatccaa 3000
tcgagcttgt?agaaggttgg?ttcagtagtt?ggaaaagctc?tattgcctct?tttttcttta 3060
tcatagggtt?aatcattgga?ctattcttgg?ttctccgagt?tggtatccat?ctttgcatta 3120
aattaaagca?caccaagaaa?agacagattt?atacagacat?agagatgaac?cgacttggaa 3180
agtaactcaa?atcctgcaca?acagattctt?catgtttgga?ccaaatcaac?ttgtgatacc 3240
atgctcaaag?aggcctcaat?tatatttgag?tttttaattt?ttatgaaaaa?aaaaaaaaaa 3300
aacggaattc?ctcgagcatg?catctagagg?gccctattct?atagtgtcac?ctaaatgcta 3360
gagctcgctg?atcagcctcg?actgtgcctt?ctagttgcca?gccatctgtt?gtttgcccct 3420
cccccgtgcc?ttccttgacc?ctggaaggtg?ccactcccac?tgtcctttcc?taataaaatg 3480
aggaaattgc?atcgcattgt?ctgagtaggt?gtcattctat?tctggggggt?ggggtggggc 3540
aggacagcaa?gggggaggat?tgggaagaca?atagcaggca?tgctggggat?gcggtgggct 3600
ctatggcttc?tgaggcggaa?agaaccagtg?gcggtaatac?ggttatccac?agaatcaggg 3660
gataacgcag?gaaagaacat?gtgagcaaaa?ggccagcaaa?aggccaggaa?ccgtaaaaag 3720
gccgcgttgc?tggcgttttt?ccataggctc?cgcccccctg?acgagcatca?caaaaatcga 3780
cgctcaagtc?agaggtggcg?aaacccgaca?ggactataaa?gataccaggc?gtttccccct 3840
ggaagctccc?tcgtgcgctc?tcctgttccg?accctgccgc?ttaccggata?cctgtccgcc 3900
tttctccctt?cgggaagcgt?ggcgctttct?catagctcac?gctgtaggta?tctcagttcg 3960
gtgtaggtcg?ttcgctccaa?gctgggctgt?gtgcacgaac?cccccgttca?gcccgaccgc 4020
tgcgccttat?ccggtaacta?tcgtcttgag?tccaacccgg?taagacacga?cttatcgcca 4080
ctggcagcag?ccactggtaa?caggattagc?agagcgaggt?atgtaggcgg?tgctacagag 4140
ttcttgaagt?ggtggcctaa?ctacggctac?actagaagga?cagtatttgg?tatctgcgct 4200
ctgctgaagc?cagttacctt?cggaaaaaga?gttggtagct?cttgatccgg?caaacaaacc 4260
accgctggta?gcggtggttt?ttttgtttgc?aagcagcaga?ttacgcgcag?aaaaaaagga 4320
tctcaagaag?atcctttgat?cttttctacg?gggtctgacg?ctcagtggaa?cgaaaactca 4380
cgttaaggga?ttttggtcat?gagattatca?aaaaggatct?tcacctagat?ccttttaaat 4440
taaaaatgaa?gttttaaatc?aatctaaagt?atatatgagt?aacctgaggc?tatggcaggg 4500
cctgccgccc?cgacgttggc?tgcgagccct?gggccttcac?ccgaacttgg?ggggtggggt 4560
ggggaaaagg?aagaaacgcg?ggcgtattgg?ccccaatggg?gtctcggtgg?ggtatcgaca 4620
gagtgccagc?cctgggaccg?aaccccgcgt?ttatgaacaa?acgacccaac?accgtgcgtt 4680
ttattctgtc?tttttattgc?cgtcatagcg?cgggttcctt?ccggtattgt?ctccttccgt 4740
gtttcagtta?gcctccccct?agggtgggcg?aagaactcca?gcatgagatc?cccgcgctgg 4800
aggatcatcc?agccggcgtc?ccggaaaacg?attccgaagc?ccaacctttc?atagaaggcg 4860
gcggtggaat?cgaaatctcg?tgatggcagg?ttgggcgtcg?cttggtcggt?catttcgaac 4920
cccagagtcc?cgctcagaag?aactcgtcaa?gaaggcgata?gaaggcgatg?cgctgcgaat 4980
cgggagcggc?gataccgtaa?agcacgagga?agcggtcagc?ccattcgccg?ccaagctctt 5040
cagcaatatc?acgggtagcc?aacgctatgt?cctgatagcg?gtccgccaca?cccagccggc 5100
cacagtcgat?gaatccagaa?aagcggccat?tttccaccat?gatattcggc?aagcaggcat 5160
cgccatgggt?cacgacgaga?tcctcgccgt?cgggcatgct?cgccttgagc?ctggcgaaca 5220
gttcggctgg?cgcgagcccc?tgatgctctt?gatcatcctg?atcgacaaga?ccggcttcca 5280
tccgagtacg?tgctcgctcg?atgcgatgtt?tcgcttggtg?gtcgaatggg?caggtagccg 5340
gatcaagcgt?atgcagccgc?cgcattgcat?cagccatgat?ggatactttc?tcggcaggag 5400
caaggtgaga?tgacaggaga?tcctgccccg?gcacttcgcc?caatagcagc?cagtcccttc 5460
ccgcttcagt?gacaacgtcg?agcacagctg?cgcaaggaac?gcccgtcgtg?gccagccacg 5520
atagccgcgc?tgcctcgtct?tgcagttcat?tcagggcacc?ggacaggtcg?gtcttgacaa 5580
aaagaaccgg?gcgcccctgc?gctgacagcc?ggaacacggc?ggcatcagag?cagccgattg 5640
tctgttgtgc?ccagtcatag?ccgaatagcc?tctccaccca?agcggccgga?gaacctgcgt 5700
gcaatccatc?ttgttcaatc?atgcgaaacg?atcctcatcc?tgtctcttga?tcgatctttg 5760
caaaagccta?ggcctccaaa?aaagcctcct?cactacttct?ggaatagctc?agaggccgag 5820
gcggcctcgg?cctctgcata?aataaaaaaa?attagtcagc?catggggcgg?agaatgggcg 5880
gaactgggcg?gagttagggg?cgggatgggc?ggagttaggg?gcgggactat?ggttgctgac 5940
taattgagat?gcatgctttg?catacttctg?cctgctgggg?agcctgggga?ctttccacac 6000
ctggttgctg?actaattgag?atgcatgctt?tgcatacttc?tgcctgctgg?ggagcctggg 6060
gactttccac?accctaactg?acacacattc?cacagctggt?tctttccgcc?tcaggactct 6120
tcctttttca?ataaatcaat?ctaaagtata?tatgagtaaa?cttggtctga?cagttaccaa 6180
tgcttaatca?gtgaggcacc?tatctcagcg?atctgtctat?ttcgttcatc?catagttgcc 6240
tgactccccg?tcgtgtagat?aactacgata?cgggagggct?taccatctgg?ccccagtgct 6300
gcaatgatac?cgcgagaccc?acgctcaccg?gctccagatt?tatcagcaat?aaaccagcca 6360
gccggaaggg?ccgagcgcag?aagtggtcct?gcaactttat?ccgcctccat?ccagtctatt 6420
aattgttgcc?gggaagctag?agtaagtagt?tcgccagtta?atagtttgcg?caacgttgtt 6480
gccattgcta?caggcatcgt?ggtgtcacgc?tcgtcgtttg?gtatggcttc?attcagctcc 6540
ggttcccaac?gatcaaggcg?agttacatga?tcccccatgt?tgtgcaaaaa?agcggttagc 6600
tccttcggtc?ctccgatcgt?tgtcagaagt?aagttggccg?cagtgttatc?actcatggtt 6660
atggcagcac?tgcataattc?tcttactgtc?atgccatccg?taagatgctt?ttctgtgact 6720
ggtgagtact?caaccaagtc?attctgagaa?tagtgtatgc?ggcgaccgag?ttgctcttgc 6780
ccggcgtcaa?tacgggataa?taccgcgcca?catagcagaa?ctttaaaagt?gctcatcatt 6840
ggaaaacgtt?cttcggggcg?aaaactctca?aggatcttac?cgctgttgag?atccagttcg 6900
atgtaaccca?ctcgtgcacc?caactgatct?tcagcatctt?ttactttcac?cagcgtttct 6960
gggtgagcaa?aaacaggaag?gcaaaatgcc?gcaaaaaagg?gaataagggc?gacacggaaa 7020
tgttgaatac?tcatactctt?cctttttcaa?tattattgaa?gcatttatca?gggttattgt 7080
ctcatgagcg?gatacatatt?tgaatgtatt?tagaaaaata?aacaaatagg?ggttccgcgc 7140
acatttcccc?gaaaagtgcc?acctgacgcg?ccctgtagcg?gcgcattaag?cgcggcgggt 7200
gtggtggtta?cgcgcagcgt?gaccgctaca?cttgccagcg?ccctagcgcc?cgctcctttc 7260
gctttcttcc?cttcctttct?cgccacgttc?gccggctttc?cccgtcaagc?tctaaatcgg 7320
gggctccctt?tagggttccg?atttagtgct?ttacggcacc?tcgaccccaa?aaaacttgat 7380
tagggtgatg?gttcacgtag?tgggccatcg?ccctgataga?cggtttttcg?ccctttgacg 7440
ttggagtcca?cgttctttaa?tagtggactc?ttgttccaaa?ctggaacaac?actcaaccct 7500
atctcggtct?attcttttga?tttataaggg?attttgccga?tttcggccta?ttggttaaaa 7560
aatgagctga?tttaacaaaa?atttaacgcg?aattttaaca?aaatattaac?gcttacaatt 7620
tac 7623
<210>75
<211>5419
<212>DNA
＜213〉artificial sequence
<220>
<223>pHYK/rev
<400>75
gcgcgcgttg?acattgatta?ttgactagtt?attaatagta?atcaattacg?gggtcattag 60
ttcatagccc?atatatggag?ttccgcgtta?cataacttac?ggtaaatggc?ccgcctggct 120
gaccgcccaa?cgacccccgc?ccattgacgt?caataatgac?gtatgttccc?atagtaacgc 180
caatagggac?tttccattga?cgtcaatggg?tggactattt?acggtaaact?gcccacttgg 240
cagtacatca?agtgtatcat?atgccaagta?cgccccctat?tgacgtcaat?gacggtaaat 300
ggcccgcctg?gcattatgcc?cagtacatga?ccttatggga?ctttcctact?tggcagtaca 360
tctacgtatt?agtcatcgct?attaccatgg?tgatgcggtt?ttggcagtac?atcaatgggc 420
gtggatagcg?gtttgactca?cggggatttc?caagtctcca?ccccattgac?gtcaatggga 480
gtttgttttg?gcaccaaaat?caacgggact?ttccaaaatg?tcgtaacaac?tccgccccat 540
tgacgcaaat?gggcggtagg?cgtgtacggt?gggaggtcta?tataagcaga?gctctctggc 600
taactagaga?acccactgct?tactggctta?tcgaaattaa?tacgactcac?tatagggaga 660
cccaagctta?gatggatgct?ggggccagat?acatgcgctt?aactgggaag?gaaaactggg 720
ttgaagtaac?catggacgga?gagaaggaaa?ggaaaagaga?aggtttcact?gcgggacagc 780
aagatataca?gaactctaag?taccccgaca?taccaacggg?tcacagtcat?catggaaaca 840
agagcagacg?tcgcaggaga?aaatcaggat?tttggcgatg?gcttagagga?atcagacaac 900
agcgaaacaa?gcgaaagagt?gacagtacag?aaagcttgga?gccgtgcctg?ggagctttgg 960
cagaactcac?cctggaagga?gccatggaaa?aggggcctgc?tgaggctgct?cgtccttccg 1020
ctgacgatgg?gaatctggat?aaatggatgg?cttggagaac?accacaaaaa?taagaattct 1080
gcagatatcc?atcacactgg?cggccgctcg?agcatgcatc?tagagggccc?tattctatag 1140
tgtcacctaa?atgctagagc?tcgctgatca?gcctcgactg?tgccttctag?ttgccagcca 1200
tctgttgttt?gcccctcccc?cgtgccttcc?ttgaccctgg?aaggtgccac?tcccactgtc 1260
ctttcctaat?aaaatgagga?aattgcatcg?cattgtctga?gtaggtgtca?ttctattctg 1320
gggggtgggg?tggggcagga?cagcaagggg?gaggattggg?aagacaatag?caggcatgct 1380
ggggatgcgg?tgggctctat?ggcttctgag?gcggaaagaa?ccagtggcgg?taatacggtt 1440
atccacagaa?tcaggggata?acgcaggaaa?gaacatgtga?gcaaaaggcc?agcaaaaggc 1500
caggaaccgt?aaaaaggccg?cgttgctggc?gtttttccat?aggctccgcc?cccctgacga 1560
gcatcacaaa?aatcgacgct?caagtcagag?gtggcgaaac?ccgacaggac?tataaagata 1620
ccaggcgttt?ccccctggaa?gctccctcgt?gcgctctcct?gttccgaccc?tgccgcttac 1680
cggatacctg?tccgcctttc?tcccttcggg?aagcgtggcg?ctttctcata?gctcacgctg 1740
taggtatctc?agttcggtgt?aggtcgttcg?ctccaagctg?ggctgtgtgc?acgaaccccc 1800
cgttcagccc?gaccgctgcg?ccttatccgg?taactatcgt?cttgagtcca?acccggtaag 1860
acacgactta?tcgccactgg?cagcagccac?tggtaacagg?attagcagag?cgaggtatgt 1920
aggcggtgct?acagagttct?tgaagtggtg?gcctaactac?ggctacacta?gaaggacagt 1980
atttggtatc?tgcgctctgc?tgaagccagt?taccttcgga?aaaagagttg?gtagctcttg 2040
atccggcaaa?caaaccaccg?ctggtagcgg?tggttttttt?gtttgcaagc?agcagattac 2100
gcgcagaaaa?aaaggatctc?aagaagatcc?tttgatcttt?tctacggggt?ctgacgctca 2160
gtggaacgaa?aactcacgtt?aagggatttt?ggtcatgaga?ttatcaaaaa?ggatcttcac 2220
ctagatcctt?ttaaattaaa?aatgaagttt?taaatcaatc?taaagtatat?atgagtaacc 2280
tgaggctatg?gcagggcctg?ccgccccgac?gttggctgcg?agccctgggc?cttcacccga 2340
acttgggggg?tggggtgggg?aaaaggaaga?aacgcgggcg?tattggcccc?aatggggtct 2400
cggtggggta?tcgacagagt?gccagccctg?ggaccgaacc?ccgcgtttat?gaacaaacga 2460
cccaacaccg?tgcgttttat?tctgtctttt?tattgccgtc?atagcgcggg?ttccttccgg 2520
tattgtctcc?ttccgtgttt?cagttagcct?ccccctaggg?tgggcgaaga?actccagcat 2580
gagatccccg?cgctggagga?tcatccagcc?ggcgtcccgg?aaaacgattc?cgaagcccaa 2640
cctttcatag?aaggcggcgg?tggaatcgaa?atctcgtgat?ggcaggttgg?gcgtcgcttg 2700
gtcggtcatt?tcgaacccca?gagtcccgct?cagaagaact?cgtcaagaag?gcgatagaag 2760
gcgatgcgct?gcgaatcggg?agcggcgata?ccgtaaagca?cgaggaagcg?gtcagcccat 2820
tcgccgccaa?gctcttcagc?aatatcacgg?gtagccaacg?ctatgtcctg?atagcggtcc 2880
gccacaccca?gccggccaca?gtcgatgaat?ccagaaaagc?ggccattttc?caccatgata 2940
ttcggcaagc?aggcatcgcc?atgggtcacg?acgagatcct?cgccgtcggg?catgctcgcc 3000
ttgagcctgg?cgaacagttc?ggctggcgcg?agcccctgat?gctcttgatc?atcctgatcg 3060
acaagaccgg?cttccatccg?agtacgtgct?cgctcgatgc?gatgtttcgc?ttggtggtcg 3120
aatgggcagg?tagccggatc?aagcgtatgc?agccgccgca?ttgcatcagc?catgatggat 3180
actttctcgg?caggagcaag?gtgagatgac?aggagatcct?gccccggcac?ttcgcccaat 3240
agcagccagt?cccttcccgc?ttcagtgaca?acgtcgagca?cagctgcgca?aggaacgccc 3300
gtcgtggcca?gccacgatag?ccgcgctgcc?tcgtcttgca?gttcattcag?ggcaccggac 3360
aggtcggtct?tgacaaaaag?aaccgggcgc?ccctgcgctg?acagccggaa?cacggcggca 3420
tcagagcagc?cgattgtctg?ttgtgcccag?tcatagccga?atagcctctc?cacccaagcg 3480
gccggagaac?ctgcgtgcaa?tccatcttgt?tcaatcatgc?gaaacgatcc?tcatcctgtc 3540
tcttgatcga?tctttgcaaa?agcctaggcc?tccaaaaaag?cctcctcact?acttctggaa 3600
tagctcagag?gccgaggcgg?cctcggcctc?tgcataaata?aaaaaaatta?gtcagccatg 3660
gggcggagaa?tgggcggaac?tgggcggagt?taggggcggg?atgggcggag?ttaggggcgg 3720
gactatggtt?gctgactaat?tgagatgcat?gctttgcata?cttctgcctg?ctggggagcc 3780
tggggacttt?ccacacctgg?ttgctgacta?attgagatgc?atgctttgca?tacttctgcc 3840
tgctggggag?cctggggact?ttccacaccc?taactgacac?acattccaca?gctggttctt 3900
tccgcctcag?gactcttcct?ttttcaataa?atcaatctaa?agtatatatg?agtaaacttg 3960
gtctgacagt?taccaatgct?taatcagtga?ggcacctatc?tcagcgatct?gtctatttcg 4020
ttcatccata?gttgcctgac?tccccgtcgt?gtagataact?acgatacggg?agggcttacc 4080
atctggcccc?agtgctgcaa?tgataccgcg?agacccacgc?tcaccggctc?cagatttatc 4140
agcaataaac?cagccagccg?gaagggccga?gcgcagaagt?ggtcctgcaa?ctttatccgc 4200
ctccatccag?tctattaatt?gttgccggga?agctagagta?agtagttcgc?cagttaatag 4260
tttgcgcaac?gttgttgcca?ttgctacagg?catcgtggtg?tcacgctcgt?cgtttggtat 4320
ggcttcattc?agctccggtt?cccaacgatc?aaggcgagtt?acatgatccc?ccatgttgtg 4380
caaaaaagcg?gttagctcct?tcggtcctcc?gatcgttgtc?agaagtaagt?tggccgcagt 4440
gttatcactc?atggttatgg?cagcactgca?taattctctt?actgtcatgc?catccgtaag 4500
atgcttttct?gtgactggtg?agtactcaac?caagtcattc?tgagaatagt?gtatgcggcg 456a
accgagttgc?tcttgcccgg?cgtcaatacg?ggataatacc?gcgccacata?gcagaacttt 4620
aaaagtgctc?atcattggaa?aacgttcttc?ggggcgaaaa?ctctcaagga?tcttaccgct 4680
gttgagatcc?agttcgatgt?aacccactcg?tgcacccaac?tgatcttcag?catcttttac 4740
tttcaccagc?gtttctgggt?gagcaaaaac?aggaaggcaa?aatgccgcaa?aaaagggaat 4800
aagggcgaca?cggaaatgtt?gaatactcat?actcttcctt?tttcaatatt?attgaagcat 4860
ttatcagggt?tattgtctca?tgagcggata?catatttgaa?tgtatttaga?aaaataaaca 4920
aataggggtt?ccgcgcacat?ttccccgaaa?agtgccacct?gacgcgccct?gtagcggcgc 4980
attaagcgcg?gcgggtgtgg?tggttacgcg?cagcgtgacc?gctacacttg?ccagcgccct 5040
agcgcccgct?cctttcgctt?tcttcccttc?ctttctcgcc?acgttcgccg?gctttccccg 5100
tcaagctcta?aatcgggggc?tccctttagg?gttccgattt?agtgctttac?ggcacctcga 5160
ccccaaaaaa?cttgattagg?gtgatggttc?acgtagtggg?ccatcgccct?gatagacggt 5220
ttttcgccct?ttgacgttgg?agtccacgtt?ctttaatagt?ggactcttgt?tccaaactgg 5280
aacaacactc?aaccctatct?cggtctattc?ttttgattta?taagggattt?tgccgatttc 5340
ggcctattgg?ttaaaaaatg?agctgattta?acaaaaattt?aacgcgaatt?ttaacaaaat 5400
attaacgctt?acaatttac 5419
<210>76
<211>5729
<212>DNA
＜213〉artificial sequence
<220>
<223>pHYK/vif
<400>76
gcgcgcgttg?acattgatta?ttgactagtt?attaatagta?atcaattacg?gggtcattag 60
ttcatagccc?atatatggag?ttccgcgtta?cataacttac?ggtaaatggc?ccgcctggct 120
gaccgcccaa?cgacccccgc?ccattgacgt?caataatgac?gtatgttccc?atagtaacgc 180
caatagggac?tttccattga?cgtcaatggg?tggactattt?acggtaaact?gcccacttgg 240
cagtacatca?agtgtatcat?atgccaagta?cgccccctat?tgacgtcaat?gacggtaaat 300
ggcccgcctg?gcattatgcc?cagtacatga?ccttatggga?ctttcctact?tggcagtaca 360
tctacgtatt?agtcatcgct?attaccatgg?tgatgcggtt?ttggcagtac?atcaatgggc 420
gtggatagcg?gtttgactca?cggggatttc?caagtctcca?ccccattgac?gtcaatggga 480
gtttgttttg?gcaccaaaat?caacgggact?ttccaaaatg?tcgtaacaac?tccgccccat 540
tgacgcaaat?gggcggtagg?cgtgtacggt?gggaggtcta?tataagcaga?gctctctggc 600
taactagaga?acccactgct?tactggctta?tcgaaattaa?tacgactcac?tatagggaga 660
cccaagcttg?gtaccgagct?cggatccgcg?atgcaaaatt?catcccgcca?ccaacaaaag 720
aaaaggaata?aaaaacctgg?accagaatta?cccttagcac?tatggatcca?tatagcagaa 780
agcattaatg?gggatagctc?atggtacata?acaatgagac?tgcaacagat?gatgtgggga 840
aaaagaggaa?ataagttaca?atataagaat?gaagacaggg?aatatgaaaa?ttgggaaatt 900
acatcatggg?gatggaaaat?gcacctaagg?agagtgaaac?aatggataca?agacaacagg 960
agaggaagcc?catggcagta?caaagtagga?ggaacatgga?aaagtatagg?agtgtggttc 1020
ctgcaagcag?gagattacag?aaaggtagac?aggcacttct?ggtgggcatg?gaggatactg 1080
atatgttcct?gcaggaaaga?aaagtttgat?ataagagaat?ttatgagagg?aagacataga 1140
tgggatttgt?gcaaatcctg?tgctcaagga?gaagtagtaa?agcatactag?aacaaaaagt 1200
ctggaaagac?tagtactgct?acagatggta?gaacagcatg?tgtttcaagt?attgccattg 1260
tggagagcca?ggagaagtag?tacaacagat?ttcccatggt?gcagggacac?aacgggatac 1320
acgcatgcgt?ggtctgtcca?ggagtgctgg?ttgatggaat?atctcttaga?ggatgagtga 1380
ccggaattct?gcagatatcc?atcacactgg?cggccgctcg?agcatgcatc?tagagggccc 1440
tattctatag?tgtcacctaa?atgctagagc?tcgctgatca?gcctcgactg?tgccttctag 1500
ttgccagcca?tctgttgttt?gcccctcccc?cgtgccttcc?ttgaccctgg?aaggtgccac 1560
tcccactgtc?ctttcctaat?aaaatgagga?aattgcatcg?cattgtctga?gtaggtgtca 1620
ttctattctg?gggggtgggg?tggggcagga?cagcaagggg?gaggattggg?aagacaatag 1680
caggcatgct?ggggatgcgg?tgggctctat?ggcttctgag?gcggaaagaa?ccagtggcgg 1740
taatacggtt?atccacagaa?tcaggggata?acgcaggaaa?gaacatgtga?gcaaaaggcc 1800
agcaaaaggc?caggaaccgt?aaaaaggccg?cgttgctggc?gtttttccat?aggctccgcc 1860
cccctgacga?gcatcacaaa?aatcgacgct?caagtcagag?gtggcgaaac?ccgacaggac 1920
tataaagata?ccaggcgttt?ccccctggaa?gctccctcgt?gcgctctcct?gttccgaccc 1980
tgccgcttac?cggatacctg?tccgcctttc?tcccttcggg?aagcgtggcg?ctttctcata 2040
gctcacgctg?taggtatctc?agttcggtgt?aggtcgttcg?ctccaagctg?ggctgtgtgc 2100
acgaaccccc?cgttcagccc?gaccgctgcg?ccttatccgg?taactatcgt?cttgagtcca 2160
acccggtaag?acacgactta?tcgccactgg?cagcagccac?tggtaacagg?attagcagag 2220
cgaggtatgt?aggcggtgct?acagagttct?tgaagtggtg?gcctaactac?ggctacacta 2280
gaaggacagt?atttggtatc?tgcgctctgc?tgaagccagt?taccttcgga?aaaagagttg 2340
gtagctcttg?atccggcaaa?caaaccaccg?ctggtagcgg?tggttttttt?gtttgcaagc 2400
agcagattac?gcgcagaaaa?aaaggatctc?aagaagatcc?tttgatcttt?tctacggggt 2460
ctgacgctca?gtggaacgaa?aactcacgtt?aagggatttt?ggtcatgaga?ttatcaaaaa 2520
ggatcttcac?ctagatcctt?ttaaattaaa?aatgaagttt?taaatcaatc?taaagtatat 2580
atgagtaacc?tgaggctatg?gcagggcctg?ccgccccgac?gttggctgcg?agccctgggc 2640
cttcacccga?acttgggggg?tggggtgggg?aaaaggaaga?aacgcgggcg?tattggcccc 2700
aatggggtct?cggtggggta?tcgacagagt?gccagccctg?ggaccgaacc?ccgcgtttat 2760
gaacaaacga?cccaacaccg?tgcgttttat?tctgtctttt?tattgccgtc?atagcgcggg 2820
ttccttccgg?tattgtctcc?ttccgtgttt?cagttagcct?ccccctaggg?tgggcgaaga 2880
actccagcat?gagatccccg?cgctggagga?tcatccagcc?ggcgtcccgg?aaaacgattc 2940
cgaagcccaa?cctttcatag?aaggcggcgg?tggaatcgaa?atctcgtgat?ggcaggttgg 3000
gcgtcgcttg?gtcggtcatt?tcgaacccca?gagtcccgct?cagaagaact?cgtcaagaag 3060
gcgatagaag?gcgatgcgct?gcgaatcggg?agcggcgata?ccgtaaagca?cgaggaagcg 3120
gtcagcccat?tcgccgccaa?gctcttcagc?aatatcacgg?gtagccaacg?ctatgtcctg 3180
atagcggtcc?gccacaccca?gccggccaca?gtcgatgaat?ccagaaaagc?ggccattttc 3240
caccatgata?ttcggcaagc?aggcatcgcc?atgggtcacg?acgagatcct?cgccgtcggg 3300
catgctcgcc?ttgagcctgg?cgaacagttc?ggctggcgcg?agcccctgat?gctcttgatc 3360
atcctgatcg?acaagaccgg?cttccatccg?agtacgtgct?cgctcgatgc?gatgtttcgc 3420
ttggtggtcg?aatgggcagg?tagccggatc?aagcgtatgc?agccgccgca?ttgcatcagc 3480
catgatggat?actttctcgg?caggagcaag?gtgagatgac?aggagatcct?gccccggcac 3540
ttcgcccaat?agcagccagt?cccttcccgc?ttcagtgaca?acgtcgagca?cagctgcgca 3600
aggaacgccc?gtcgtggcca?gccacgatag?ccgcgctgcc?tcgtcttgca?gttcattcag 3660
ggcaccggac?aggtcggtct?tgacaaaaag?aaccgggcgc?ccctgcgctg?acagccggaa 3720
cacggcggca?tcagagcagc?cgattgtctg?ttgtgcccag?tcatagccga?atagcctctc 3780
cacccaagcg?gccggagaac?ctgcgtgcaa?tccatcttgt?tcaatcatgc?gaaacgatcc 3840
tcatcctgtc?tcttgatcga?tctttgcaaa?agcctaggcc?tccaaaaaag?cctcctcact 3900
acttctggaa?tagctcagag?gccgaggcgg?cctcggcctc?tgcataaata?aaaaaaatta 3960
gtcagccatg?gggcggagaa?tgggcggaac?tgggcggagt?taggggcggg?atgggcggag 4020
ttaggggcgg?gactatggtt?gctgactaat?tgagatgcat?gctttgcata?ctcctgcctg 4080
ctggggagcc?tggggacttt?ccacacctgg?ttgctgacta?attgagatgc?atgctttgca 4140
tacttctgcc?tgctggggag?cctggggact?ttccacaccc?taactgacac?acattccaca 4200
gctggttctt?tccgcctcag?gactcttcct?ttttcaataa?atcaatctaa?agtatatatg 4260
agtaaacttg?gtctgacagt?taccaatgct?taatcagtga?ggcacctatc?tcagcgatct 4320
gtctatttcg?ttcatccata?gttgcctgac?tccccgtcgt?gtagataact?acgatacggg 4380
agggcttacc?atctggcccc?agtgctgcaa?tgataccgcg?agacccacgc?tcaccggctc 4440
cagatttatc?agcaataaac?cagccagccg?gaagggccga?gcgcagaagt?ggtcctgcaa 4500
ctttatccgc?ctccatccag?tctattaatt?gttgccggga?agctagagta?agtagttcgc 4560
cagttaatag?tttgcgcaac?gttgttgcca?ttgctacagg?catcgtggtg?tcacgctcgt 4620
cgtttggtat?ggcttcattc?agctccggtt?cccaacgatc?aaggcgagtt?acatgatccc 4680
ccatgttgtg?caaaaaagcg?gttagctcct?tcggtcctcc?gatcgttgtc?agaagtaagt 4740
tggccgcagt?gttatcactc?atggttatgg?cagcactgca?taattctctt?actgtcatgc 4800
catccgtaag?atgcttttct?gtgactggtg?agtactcaac?caagtcattc?tgagaatagt 4860
gtatgcggcg?accgagttgc?tcttgcccgg?cgtcaatacg?ggataatacc?gcgccacata 4920
gcagaacttt?aaaagtgctc?atcattggaa?aacgttcttc?ggggcgaaaa?ctctcaagga 4980
tcttaccgct?gttgagatcc?agttcgatgt?aacccactcg?tgcacccaac?tgatcttcag 5040
catcttttac?tttcaccagc?gtttctgggt?gagcaaaaac?aggaaggcaa?aatgccgcaa 5100
aaaagggaat?aagggcgaca?cggaaatgtt?gaatactcat?actcttcctt?tttcaatatt 5160
attgaagcat?ttatcagggt?tattgtctca?tgagcggata?catatttgaa?tgtatttaga 5220
aaaataaaca?aataggggtt?ccgcgcacat?ttccccgaaa?agtgccacct?gacgcgccct 5280
gtagcggcgc?attaagcgcg?gcgggtgtgg?tggttacgcg?cagcgtgacc?gctacacttg 5340
ccagcgccct?agcgcccgct?cctttcgctt?tcttcccttc?ctttctcgcc?acgttcgccg 5400
gctttccccg?tcaagctcta?aatcgggggc?tccctttagg?gttccgattt?agtgctttac 5460
ggcacctcga?ccccaaaaaa?cttgattagg?gtgatggttc?acgtagtggg?ccatcgccct 5520
gatagacggt?ttttcgccct?ttgacgttgg?agtccacgtt?ctttaatagt?ggactcttgt 5580
tccaaactgg?aacaacactc?aaccctatct?cggtctattc?ttttgattta?taagggattt 5640
tgccgatttc?ggcctattgg?ttaaaaaatg?agctgattta?acaaaaattt?aacgcgaatt 5700
ttaacaaaat?attaacgctt?acaatttac 5729
<210>77
<211>9446
<212>DNA
＜213〉artificial sequence
<220>
<223>pMGP/RRE
<400>77
aacaggaaag?ttccattgga?gccaagtaca?ttgagtcaat?agggactttc?caatgggttt 60
tgcccagtac?ataaggtcaa?tgggaggtaa?gccaatgggt?ttttcccatt?actggcacgt 120
atactgagtc?attagggact?ttccaatggg?ttttgcccag?tacataaggt?caataggggt 180
gaatcaacag?gaaagtccca?ttggagccaa?gtacactgag?tcaataggga?ctttccattg 240
ggttttgccc?agtacaaaag?gtcaataggg?ggtgagtcaa?tgggtttttc?ccattattgg 300
cacgtacata?aggtcaatag?gggtgagtca?ttgggttttt?ccagccaatt?taattaaaac 360
gccatgtact?ttcccaccat?tgacgtcaat?gggctattga?aactaatgca?acgtgacctt 420
taaacggtac?tttcccatag?ctgattaatg?ggaaagtacc?gttctcgagc?caatacacgt 480
caatgggaag?tgaaagggca?gccaaaacgt?aacaccgccc?cggttttccc?tggaaattcc 540
atattggcac?gcattctatt?ggctgagctg?cgttcacgtg?ggtataagag?gcgcgaccag 600
cgtcggtacc?gtcgcagtct?tcggtctgac?caccgtagaa?cgcagagctc?ctcgctgcag 660
gcaagcttgg?taccgagctc?ggatcccggg?gaggtaccaa?aatccgccat?ggtgagtcta 720
gatagagaca?tggcgaggca?agtctccggg?gggaaaagag?attatcctga?gctcgaaaaa 780
tgtatcaagc?atgcatgcaa?gataaaagtt?cgactcagag?gggagcactt?gacagaagga 840
aattgtttat?ggtgccttaa?aacattagat?tacatgtttg?aggaccataa?agaggaacct 900
tggacaaaag?taaaatttag?gacaatatgg?cagaaggtga?agaatctaac?tcctgaggag 960
agtaacaaaa?aagactttat?gtctttgcag?gccacattag?cgggtctaat?gtgttgccaa 1020
atggggatga?gacctgagac?attgcaagat?gcaatggcta?cagtaatcat?gaaagatggg 1080
ttactggaac?aagaggaaaa?gaaggaagac?aaaagagaaa?aggaagagag?tgtcttccca 1140
atagtagtgc?aagcagcagg?agggagaagc?tggaaagcag?tagattctgt?aatgttccag 1200
caactgcaaa?cagtagcaat?gcagcatggc?ctcgtgtctg?aggactttga?aaggcagttg 1260
gcatattatg?ctactacctg?gacaagtaaa?gacatactag?aagtattggc?catgatgcct 1320
ggaaatagag?ctcaaaagga?gttaattcaa?gggaaattaa?atgaagaagc?agaaaggtgg 1380
agaaggaata?atccaccacc?tccagcagga?ggaggattaa?cagtggatca?aattatgggg 1440
gtaggacaaa?caaatcaagc?agcagcacaa?gctaacatgg?atcaggcaag?gcaaatatgc 1500
ctgcaatggg?taataaatgc?attaagagca?gtaagacata?tggcgcacag?gccagggaat 1560
ccaatgctag?taaagcaaaa?aacgaatgag?ccatatgaag?attttgcagc?aagactgcta 1620
gaagcaatag?atgcagagcc?agttacacag?cctataaaag?attatctaaa?gctaacacta 1680
tcttatacaa?atgcatcagc?agattgtcag?aagcaaatgg?atagaacact?aggacaaaga 1740
gtacaacaag?ctagtgtaga?agaaaaaatg?caagcatgta?gagatgtggg?atcagaaggg 1800
ttcaaaatgc?aattgttagc?acaagcatta?aggccaggaa?aaggaaaagg?gaatggacag 1860
ccacaaaggt?gttacaactg?tggaaaaccg?ggacatcaag?caaggcaatg?tagacaagga 1920
atcatatgtc?acaactgtgg?aaagagagga?catatgcaaa?aagaatgcag?aggaaagaga 1980
gacataaggg?gaaaacagca?gggaaacggg?aggaggggga?tacgtgtggt?gccgtccgct 2040
cctcctatgg?aataacttca?gcaccaccta?tggttcaggt?ccgcataggt?tcccagcaga 2100
ggaacttgtt?atttgatacc?ggggcggacc?gaactatagt?tagatggcat?gagggctcgg 2160
gaaacccagc?cggaaggata?aaactgcaag?gaataggagg?aatagtagaa?ggagaaaaat 2220
ggaataatgt?agaattagaa?tataaaggag?aaacaagaaa?gggaacaata?gtagtgttac 2280
cacaaagtcc?agtagaagta?ttaggacgag?ataacatggc?ccgatttgga?ataaagataa 2340
taatggcaaa?tttagaggaa?aaaagaatcc?caattacaaa?agtaaaattg?aaagagggat 2400
gtacgggtcc?acatgtccca?caatggccat?taacagaaga?gaaattaaaa?ggtctaacag 2460
aaatcataga?taaattagtg?gaagaaggaa?aactaggaaa?ggcaccccca?cattggacat 2520
gtaatactcc?aatcttttgc?ataaaaaaga?aatcagggaa?gtggagaatg?ttaatagatt 2580
tcagagaatt?gaacaaacag?acagaagatt?taacagaagc?gcagttagga?ctcccgcatc 2640
cgggaggact?acaaaagaaa?aaacatgtta?caatattgga?cataggagat?gcatatttta 2700
ctatacccct?atatgaacca?tatcgagagt?acacatgttt?tactctatta?agtcctaata 2760
atctaggacc?atgtaaaaga?tactattgga?aagtgctgcc?acaaggttgg?aaattgagtc 2820
catctgtata?tcaatttact?atgcaggaga?tcttagagga?ttggatacag?cagcatccag 2880
aaattcaatt?tggcatatat?atggatgata?tttacatagg?aagtgattta?gaaattaaaa 2940
agcatagaga?aatagtgaaa?gatttagcca?attatattgc?ccaatatgga?ttcactctgc 3000
cagaagagaa?gagacaaaag?ggatatccag?caaaatggct?aggatttgaa?ctacacccgc 3060
agacctggaa?atttcagaag?catacattac?ctgaattaac?aaagggaaca?ataacattaa 3120
ataaattaca?gaaattagta?ggagaattag?tatggagaca?atccataatt?gggaaaagca 3180
ttcctaacat?tctgaaatta?atggaaggag?atagagaatt?acaaagtgaa?agaaaaattg 3240
aagaagtaca?tgtgaaagaa?tgggaagcat?gtaggaaaaa?attagaagaa?atggaaggaa 3300
attattataa?taaagacaaa?gatgtctatg?gacaattggc?ttggggagac?aaagctatag 3360
aatatatagt?gtatcaggag?aaagggaaac?cattatgggt?aaatgtggtt?cacaatataa 3420
agaacctaag?catcccgcaa?caggttatta?aagcagcgca?aaaattaacc?caagaagtca 3480
tcattaggac?aggaaaaata?ccatggatat?tgttgccagg?gaaagaagaa?gattggagac 3540
tagaattgca?attagggaac?atcacatgga?tgccaaaatt?ttggtcctgt?tatcgaggac 3600
atacaagatg?gagaaaaaga?aatataatag?aagaagtagt?agaagggcct?acatattata 3660
cagatggagg?aaaaaagaat?aaagtaggaa?gtctagggtt?catagtatca?acaggggaaa 3720
aatttagaaa?gcatgaagag?ggcacaaacc?agcaactaga?attaagagcc?atagaggaag 3780
ctctaaaaca?agggcctcaa?acaatgaatt?tagtaacaga?tagtagatat?gcatttgaat 3840
ttttattaag?aaattgggat?gaagaagtaa?taaagaatcc?aattcaagca?agaattatgg 3900
aaattgccca?caagaaagat?aggataggag?tgcattgggt?gccaggacat?aaagggattc 3960
cccaaaatga?agaaatagac?aaatatattt?cggaaatatt?tcttgcaaaa?gaaggagaag 4020
gaattctccc?aaaaagagaa?gaggatgcag?ggtatgattt?aatatgccca?gaagaggtta 4080
ccatagagcc?aggacaagtg?aaatgcatcc?ccatagagct?aagattaaat?ttaaagaaat 4140
cacaatgggc?tatgattgct?acaaaaagca?gcatggctgc?caaaggagtg?ttcacacaag 4200
gaggaatcat?agactcagga?tatcagggac?aaatacaggt?aataatgtat?aatagcaata 4260
aaatagcagt?agtcataccc?caagggagaa?aatttgcaca?attaatatta?atggataaaa 4320
agcatgaaaa?attggaaccc?tggggggaaa?gcagaaaaac?agaaagggga?gaaaaaggat 4380
ttgggtctac?aggaatgtat?tggatagaaa?atattcctct?ggcagaggaa?gaccacacaa 4440
aatggcatca?agatgcccga?tcattgcatc?tagaatttga?aattccaaga?acagcagcag 4500
aagacatagt?aaatcaatgt?gaaatatgca?aagaaggcag?gacacctgca?gtaattagag 4560
gcggaaacaa?aaggggggta?gatcattggc?aagtggatta?tacccattat?gaaaatatca 4620
tactattagt?atgggtagaa?acaaattcag?gactaatata?tgcagaaaaa?gtaaaaggag 4680
aatcagggca?agaattcaga?ataaaagtga?tgcaatggta?tgcattattt?ggtccagagt 4740
cattgcagtc?agacaatgga?cctgcatttg?cagcagagcc?cacacagctg?ttaatgcaat 4800
acctaggagt?aaaacacaca?acaggcatac?cttggaatcc?acagtctcag?gctatagtag 4860
aaagggcaca?tcaactattg?aaaagcactt?taaagaagtt?ccagccacaa?tttgtcgctg 4920
tagaatcagc?catagcagca?gccctagtcg?ccataaatat?aaaaagaaag?ggtgggctgg 4980
ggacaagccc?tatggatatt?tttatatata?ataaagaaca?gaaaagaata?aataataaat 5040
ataataaaaa?ttctcaaaaa?attcaattct?gttattacag?aataaggaaa?agaggacatc 5100
caggagagtg?gaaaggacca?acccaggtac?tgtggaaagg?ggaaggagca?attgtggtaa 5160
aggatataga?aagtgaaaag?tatttagtaa?taccttacaa?agatgcaaaa?ttcatcccgc 5220
caccaacaaa?agaaaaggaa?taaaaaacct?ggaccagaat?tacccttagc?actatggatc 5280
cactagtaac?ggccgccagt?gtgctggaat?tctgcagata?tccatcacac?tggcggccgg 5340
gctgcaggaa?ttcgatagaa?aagatatcaa?aaacaagaat?cataaacagg?aaaaaaagag 5400
aactcagcca?caagaggaag?aagagaggcg?ttggcttggt?cattatgcta?gttatcatgg 5460
caatagtagc?tgccgcaggg?gcttctctgg?gagtcgcaaa?cgcgattcag?cagtcttaca 5520
ctaaggcagc?tgtccagacc?cttgctaatg?caactgctgc?acagcaggat?gtgttagagg 5580
caacctatgc?catggtacag?catgtggcta?aaggcgtacg?aatcttggaa?gctcgagtgg 5640
ctcgagtgga?agctatcaca?gatagaatag?cggccgccca?tcaagcttat?cgataccgtc 5700
ggccgctcga?gcatgcatct?agagggccct?attctatagt?gtcacctaaa?tgctagagct 5760
cgctgatcag?cctcgactgt?gccttctagt?tgccagccat?ctgttgtttg?cccctccccc 5820
gtgccttcct?tgaccctgga?aggtgccact?cccactgtcc?tttcctaata?aaatgaggaa 5880
attgcatcgc?attgtctgag?taggtgtcat?tctattctgg?ggggtggggt?ggggcaggac 5940
agcaaggggg?aggattggga?agacaatagc?aggcatgctg?gggatgcggt?gggctctatg 6000
gcttctgagg?cggaaagaac?cagtggcggt?aatacggtta?tccacagaat?caggggataa 6060
cgcaggaaag?aacatgtgag?caaaaggcca?gcaaaaggcc?aggaaccgta?aaaaggccgc 6120
gttgctggcg?tttttccata?ggctccgccc?ccctgacgag?catcacaaaa?atcgacgctc 6180
aagtcagagg?tggcgaaacc?cgacaggact?ataaagatac?caggcgtttc?cccctggaag 6240
ctccctcgtg?cgctctcctg?ttccgaccct?gccgcttacc?ggatacctgt?ccgcctttct 6300
cccttcggga?agcgtggcgc?tttctcatag?ctcacgctgt?aggtatctca?gttcggtgta 6360
ggtcgttcgc?tccaagctgg?gctgtgtgca?cgaacccccc?gttcagcccg?accgctgcgc 6420
cttatccggt?aactatcgtc?ttgagtccaa?cccggtaaga?cacgacttat?cgccactggc 6480
agcagccact?ggtaacagga?ttagcagagc?gaggtatgta?ggcggtgcta?cagagttctt 6540
gaagtggtgg?cctaactacg?gctacactag?aaggacagta?tttggtatct?gcgctctgct 6600
gaagccagtt?accttcggaa?aaagagttgg?tagctcttga?tccggcaaac?aaaccaccgc 6660
tggtagcggt?ggtttttttg?tttgcaagca?gcagattacg?cgcagaaaaa?aaggatctca 6720
agaagatcct?ttgatctttt?ctacggggtc?tgacgctcag?tggaacgaaa?actcacgtta 6780
agggattttg?gtcatgagat?tatcaaaaag?gatcttcacc?tagatccttt?taaattaaaa 6840
atgaagtttt?aaatcaatct?aaagtatata?tgagtaacct?9aggctatgg?cagggcctgc 6900
cgccccgacg?ttggctgcga?gccctgggcc?ttcacccgaa?cttggggggt?ggggtgggga 6960
aaaggaagaa?acgcgggcgt?attggcccca?atggggtctc?ggtggggtat?cgacagagtg 7020
ccagccctgg?gaccgaaccc?cgcgtttatg?aacaaacgac?ccaacaccgt?gcgttttatt 7080
ctgtcttttt?attgccgtca?tagcgcgggt?tccttccggt?attgtctcct?tccgtgtttc 7140
agttagcctc?cccctagggt?gggcgaagaa?ctccagcatg?agatccccgc?gctggaggat 7200
catccagccg?gcgtcccgga?aaacgattcc?gaagcccaac?ctttcataga?aggcggcggt 7260
ggaatcgaaa?tctcgtgatg?gcaggttggg?cgtcgcttgg?tcggtcattt?cgaaccccag 7320
agtcccgctc?agaagaactc?gtcaagaagg?cgatagaagg?cgatgcgctg?cgaatcggga 7380
gcggcgatac?cgtaaagcac?gaggaagcgg?tcagcccatt?cgccgccaag?ctcttcagca 7440
atatcacggg?tagccaacgc?tatgtcctga?tagcggtccg?ccacacccag?ccggccacag 7500
tcgatgaatc?cagaaaagcg?gccattttcc?accatgatat?tcggcaagca?ggcatcgcca 7560
tgggtcacga?cgagatcctc?gccgtcgggc?atgctcgcct?tgagcctggc?gaacagttcg 7620
gctggcgcga?gcccctgatg?ctcttgatca?tcctgatcga?caagaccggc?ttccatccga 7680
gtacgtgctc?gctcgatgcg?atgtttcgct?tggtggtcga?atgggcaggt?agccggatca 7740
agcgtatgca?gccgccgcat?tgcatcagcc?atgatggata?ctttctcggc?aggagcaagg 7800
tgagatgaca?ggagatcctg?ccccggcact?tcgcccaata?gcagccagtc?ccttcccgct 7860
tcagtgacaa?cgtcgagcac?agctgcgcaa?ggaacgcccg?tcgtggccag?ccacgatagc 7920
cgcgctgcct?cgtcttgcag?ttcattcagg?gcaccggaca?ggtcggtctt?gacaaaaaga 7980
accgggcgcc?cctgcgctga?cagccggaac?acggcggcat?cagagcagcc?gattgtctgt 8040
tgtgcccagt?catagccgaa?tagcctctcc?acccaagcgg?ccggagaacc?tgcgtgcaat 8100
ccatcttgtt?caatcatgcg?aaacgatcct?catcctgtct?cttgatcgat?ctttgcaaaa 8160
gcctaggcct?ccaaaaaagc?ctcctcacta?cttctggaat?agctcagagg?ccgaggcggc 8220
ctcggcctct?gcataaataa?aaaaaattag?tcagccatgg?ggcggagaat?gggcggaact 8280
gggcggagtt?aggggcggga?tgggcggagt?taggggcggg?actatggttg?ctgactaatt 8340
gagatgcatg?ctttgcatac?ttctgcctgc?tggggagcct?ggggactttc?cacacctggt 8400
tgctgactaa?ttgagatgca?tgctttgcat?acttctgcct?gctggggagc?ctggggactt 8460
tccacaccct?aactgacaca?cattccacag?ctggttcttt?ccgcctcagg?actcttcctt 8520
tttcaataaa?tcaatctaaa?gtatatatga?gtaaacttgg?tctgacagtt?accaatgctt 8580
aatcagtgag?gcacctatct?cagcgatctg?tctatttcgt?tcatccatag?ttgcctgact 8640
ccccgtcgtg?tagataacta?cgatacggga?gggcttacca?tctggcccca?gtgctgcaat 8700
gataccgcga?gacccacgct?caccggctcc?agatttatca?gcaataaacc?agccagccgg 8760
aagggccgag?cgcagaagtg?gtcctgcaac?tttatccgcc?tccatccagt?ctattaattg 8820
ttgccgggaa?gctagagtaa?gtagttcgcc?agttaatagt?ttgcgcaacg?ttgttgccat 8880
tgctacaggc?atcgtggtgt?cacgctcgtc?gtttggtatg?gcttcattca?gctccggttc 8940
ccaacgatca?aggcgagtta?catgatcccc?catgttgtgc?aaaaaagcgg?ttagctcctt 9000
cggtcctccg?atcgttgtca?gaagtaagtt?ggccgcagtg?ttatcactca?tggttatggc 9060
agcactgcat?aattctctta?ctgtcatgcc?atccgtaaga?tgcttttctg?tgactggtga 9120
gtactcaacc?aagtcattct?gagaatagtg?tatgcggcga?ccgagttgct?cttgcccggc 9180
gtcaatacgg?gataataccg?cgccacatag?cagaacttta?aaagtgctca?tcattggaaa 9240
acgttcttcg?gggcgaaaac?tctcaaggat?cttaccgctg?ttgagatcca?gttcgatgta 9300
acccactcgt?gcacccaact?gatcttcagc?atcttttact?ttcaccagcg?tttctgggtg 9360
agcaaaaaca?ggaaggcaaa?atgccgcaaa?aaagggaata?agggcgacac?ggaaatgttg 9420
aatactcata?ctcttccttt?ttcaat 9446
<210>78
<211>7856
<212>DNA
＜213〉artificial sequence
<220>
<223>pCAH/SINd60/hlacZ
<400>78
gttgacattg?attattgact?agttattaat?agtaatcaat?tacggggtca?ttagttcata 60
gcccatatat?ggagttccgc?gttacataac?ttacggtaaa?tggcccgcct?ggctgaccgc 120
ccaacgaccc?ccgcccattg?acgtcaataa?tgacgtatgt?tcccatagta?acgccaatag 180
ggactttcca?ttgacgtcaa?tgggtggagt?atttacggta?aactgcccac?ttggcagtac 240
atcaagtgta?tcatatgcca?agtacgcccc?ctattgacgt?caatgacggt?aaatggcccg 300
cctggcatta?tgcccagtac?atgaccttat?gggactttcc?tacttggcag?tacatctacg 360
tattagtcat?cgctattacc?atggtgatgc?ggttttggca?gtacatcaat?gggcgtggat 420
agcggtttga?ctcacgggga?tttccaagtc?tccaccccat?tgacgtcaat?gggagtttgt 480
tttggcacca?aaatcaacgg?gactttccaa?aatgtcgtaa?caactccgcc?ccattgacgc 540
aaatgggcgg?taggcgtgta?cggtgggagg?tctatataag?cagagctcaa?gcttgctgct 600
tgcacttcag?agttctagga?gagtccctcc?tagtctctcc?tctccgagga?ggtaccgaga 660
cctcaaaata?aaggagtgat?tgccttactg?ccgagtggag?agtgattact?gagcggccgg 720
tgtatcggga?gtcgtccctt?aatctgtgca?ataccagagc?ggctctcgca?gctggcgccc 780
aacgtggggc?ccgaggagaa?gaaaagaaag?cggccctgag?aactcggctt?ctgaaaaaga 840
ggaagaggac?aagttgctat?agcaacaaga?gagaagaagt?agagcaaagg?tccagtggct 900
cggaaaaaga?ggaactgaaa?cttcggggac?gcctgaagga?gtaaggtaag?tgactctgct 960
gtacgcgggg?cgaggcagag?gtttccttct?aaattgaaag?agaagtgttg?ctgcgagagg 1020
tcttggtggt?cgagaatcct?gtacaaaaaa?aaggagggat?ctcggtcagg?accaggaccc 1080
ctgggagtaa?tacaacagca?acaccgtaag?aaaatccgcc?taggtgagtc?tagatagaga 1140
ctaggcgagg?caagtctccg?gggggaaaag?agattatcct?gcaggaattc?gattctagag 1200
gtgatagaaa?tgccagaaaa?ctatgcaaaa?acaagaatca?taaacaggaa?aaaaagagaa 1260
ctcagccaca?agaggaagaa?gagaggcgtt?ggcttggtca?ttatgctagt?tatcatggca 1320
atagtagctg?ccgcaggggc?ttctctggga?gtcgcaaacg?cgattcagca?gtcttacact 1380
aaggcagctg?tccagaccct?tgctaatgca?actgctgcac?agcaggatgt?gttagaggca 1440
acctatgcca?tggtacagca?tgtggctaaa?ggcgtacgaa?tcttggaagc?tcgagtggct 1500
cgagtggaag?ctatcacaga?tagaataatg?ctataccaag?aattggattg?ttggcactag 1560
gatccatcag?ccaccattaa?cgcttacaat?ttacgcgcgc?gttgacattg?attattgact 1620
agttattaat?agtaatcaat?tacggggtca?ttagttcata?gcccatatat?ggagttccgc 1680
gttacataac?ttacggtaaa?tggcccgcct?ggctgaccgc?ccaacgaccc?ccgcccattg 1740
acgtcaataa?tgacgtatgt?tcccatagta?acgccaatag?ggactttcca?ttgacgtcaa 1800
tgggtggact?atttacggta?aactgcccac?ttggcagtac?atcaagtgta?tcatatgcca 1860
agtacgcccc?ctattgacgt?caatgacggt?aaatggcccg?cctggcatta?tgcccagtac 1920
atgaccttat?gggactttcc?tacttggcag?tacatctacg?tattagtcat?cgctattacc 1980
atggtgatgc?ggttttggca?gtacatcaat?gggcgtggat?agcggtttga?ctcacgggga 2040
tttccaagtc?tccaccccat?tgacgtcaat?gggagtttgt?tttggcacca?aaatcaacgg 2100
gactttccaa?aatgtcgtaa?caactccgcc?ccattgacgc?aaatgggcgg?taggcgtgta 2160
cggtgggagg?tctatataag?cagagctctc?tggctaacta?gagaacccac?tgcttactgg 2220
cttatcgaaa?ttaatacgac?tcactatagg?gagacccaag?ctgcttacca?tggggggttc 2280
tcatcatcat?catcatcatg?gtatggctag?catgactggt?ggacagcaaa?tgggtcggga 2340
tctgtacgac?gatgacgata?aggtacctaa?ggatcagctt?ggagttgatc?ccgtcgtttt 2400
acaacgtcgt?gactgggaaa?accctggcgt?tacccaactt?aatcgccttg?cagcacatcc 2460
ccctttcgcc?agctggcgta?atagcgaaga?ggcccgcacc?gatcgccctt?cccaacagtt 2520
gcgcagcctg?aatggcgaat?ggcgctttgc?ctggtttccg?gcaccagaag?cggtgccgga 2580
aagctggctg?gagtgcgatc?ttcctgaggc?cgatactgtc?gtcgtcccct?caaactggca 2640
gatgcacggt?tacgatgcgc?ccatctacac?caacgtaacc?tatcccatta?cggtcaatcc 2700
gccgtttgtt?cccacggaga?atccgacggg?ttgttactcg?ctcacattta?atgttgatga 2760
aagctggcta?caggaaggcc?agacgcgaat?tatttttgat?ggcgttaact?cggcgtttca 2820
tctgtggtgc?aacgggcgct?gggtcggtta?cggccaggac?agtcgtttgc?cgtctgaatt 2880
tgacctgagc?gcatttttac?gcgccggaga?aaaccgcctc?gcggtgatgg?tgctgcgttg 2940
gagtgacggc?agttatctgg?aagatcagga?tatgtggcgg?atgagcggca?ttttccgtga 3000
cgtctcgttg?ctgcataaac?cgactacaca?aatcagcgat?ttccatgttg?ccactcgctt 3060
taatgatgat?ttcagccgcg?ctgtactgga?ggctgaagtt?cagatgtgcg?gcgagttgcg 3120
tgactaccta?cgggtaacag?tttctttatg?gcagggtgaa?acgcaggtcg?ccagcggcac 3180
cgcgcctttc?ggcggtgaaa?ttatcgatga?gcgtggtggt?tatgccgatc?gcgtcacact 3240
acgtctgaac?gtcgaaaacc?cgaaactgtg?gagcgccgaa?atcccgaatc?tctatcgtgc 3300
ggtggttgaa?ctgcacaccg?ccgacggcac?gctgattgaa?gcagaagcct?gcgatgtcgg 3360
tttccgcgag?gtgcggattg?aaaatggtct?gctgctgctg?aacggcaagc?cgttgctgat 3420
tcgaggcgtt?aaccgtcacg?agcatcatcc?tctgcatggt?caggtcatgg?atgagcagac 3480
gatggtgcag?gatatcctgc?tgatgaagca?gaacaacttt?aacgccgtgc?gctgttcgca 3540
ttatccgaac?catccgctgt?ggtacacgct?gtgcgaccgc?tacggcctgt?atgtggtgga 3600
tgaagccaat?attgaaaccc?acggcatggt?gccaatgaat?cgtctgaccg?atgatccgcg 3660
ctggctaccg?gcgatgagcg?aacgcgtaac?gcgaatggtg?cagcgcgatc?gtaatcaccc 3720
gagtgtgatc?atctggtcgc?tggggaatga?atcaggccac?ggcgctaatc?acgacgcgct 3780
gtatcgctgg?atcaaatctg?tcgatccttc?ccgcccggtg?cagtatgaag?gcggcggagc 3840
cgacaccacg?gccaccgata?ttatttgccc?gatgtacgcg?cgcgtggatg?aagaccagcc 3900
cttcccggct?gtgccgaaat?ggtccatcaa?aaaatggctt?tcgctacctg?gagagacgcg 3960
cccgctgatc?ctttgcgaat?acgcccacgc?gatgggtaac?agtcttggcg?gtttcgctaa 4020
atactggcag?gcgtttcgtc?agtatccccg?tttacagggc?ggcttcgtct?gggactgggt 4080
ggatcagtcg?ctgattaaat?atgatgaaaa?cggcaacccg?tggtcggctt?acggcggtga 4140
ttttggcgat?acgccgaacg?atcgccagtt?ctgtatgaac?ggtctggtct?ttgccgaccg 4200
cacgccgcat?ccagcgctga?cggaagcaaa?acaccagcag?cagtttttcc?agttccgttt 4260
atccgggcaa?accatcgaag?tgaccagcga?atacctgttc?cgtcatagcg?ataacgagct 4320
cctgcactgg?atggtggcgc?tggatggtaa?gccgctggca?agcggtgaag?tgcctctgga 4380
tgtcgctcca?caaggtaaac?agttgattga?actgcctgaa?ctaccgcagc?cggagagcgc 4440
cgggcaactc?tggctcacag?tacgcgtagt?gcaaccgaac?gcgaccgcat?ggtcagaagc 4500
cgggcacatc?agcgcctggc?agcagtggcg?tctggcggaa?aacctcagtg?tgacgctccc 4560
cgccgcgtcc?cacgccatcc?cgcatctgac?caccagcgaa?atggattttt?gcatcgagct 4620
gggtaataag?cgttggcaat?ttaaccgcca?gtcaggcttt?ctttcacaga?tgtggattgg 4680
cgataaaaaa?caactgctga?cgccgctgcg?cgatcagttc?acccgtgcac?cgctggataa 4740
cgacattggc?gtaagtgaag?cgacccgcat?tgaccctaac?gcctgggtcg?aacgctggaa 4800
ggcggcgggc?cattaccagg?ccgaagcagc?gttgttgcag?tgcacggcag?atacacttgc 4860
tgatgcggtg?ctgattacga?ccgctcacgc?gtggcagcat?caggggaaaa?ccttatttat 4920
cagccggaaa?acctaccgga?ttgatggtag?tggtcaaatg?gcgattaccg?ttgatgttga 4980
agtggcgagc?gatacaccgc?atccggcgcg?gattggcctg?aactgccagc?tggcgcaggt 5040
agcagagcgg?gtaaactggc?tcggattagg?gccgcaagaa?aactatcccg?accgccttac 5100
tgccgcctgt?tttgaccgct?gggatctgcc?attgtcagac?atgtataccc?cgtacgtctt 5160
cccgagcgaa?aacggtctgc?gctgcgggac?gcgcgaattg?aattatggcc?cacaccagtg 5220
gcgcggcgac?ttccagttca?acatcagccg?ctacagtcaa?cagcaactga?tggaaaccag 5280
ccatcgccat?ctgctgcacg?cggaagaagg?cacatggctg?aatatcgacg?gtttccatat 5340
ggggattggt?ggcgacgact?cctggagccc?gtcagtatcg?gcggaattac?agctgagcgc 5400
cggtcgctac?cattaccagt?tggtctggtg?tcaaaaataa?taaagccgaa?ttctgcagat 5460
atccagcaca?gtggcggccg?ctagcacaaa?aataaaaaaa?gaaagggtga?ctgtgagaca 5520
tgggctaaag?aggagcggcc?gctcgagtct?agaactagtg?gatcagcttt?gctgcttgca 5580
cttcagagtt?ctaggagagt?ccctcctagt?ctctcctctc?cgaggaggta?ccgagacctc 5640
aaaataaagg?agtgattgcc?ttactgccga?gtggagagtg?attactgagc?ggccggtgta 5700
tcgggagtcg?tcccttaatc?tgtgcaatac?cagagcggct?ctcgcagccg?acctcgaggg 5760
ggggccctat?tctatagtgt?cacctaaatg?ctagagctcg?ctgatcagcc?tcgactgtgc 5820
cttctagttg?ccagccatct?gttgtttgcc?cctcccccgt?gccttccttg?accctggaag 5880
gtgccactcc?cactgtcctt?tcctaataaa?atgaggaaat?tgcatcgcat?tgtctgagta 5940
ggtgtcattc?tattctgggg?ggtggggtgg?ggcaggacag?caagggggag?gattgggaag 6000
acaatagcag?gcatgctggg?gatgcggtgg?gctctatggc?ttctgaggcg?gaaagaacca 6060
gtggcggtaa?tacggttatc?cacagaatca?ggggataacg?caggaaagaa?catgtgagca 6120
aaaggccagc?aaaaggccag?gaaccgtaaa?aaggccgcgt?tgctggcgtt?tttccatagg 6180
ctccgccccc?ctgacgagca?tcacaaaaat?cgacgctcaa?gtcagaggtg?gcgaaacccg 6240
acaggactat?aaagatacca?ggcgtttccc?cctggaagct?ccctcgtgcg?ctctcctgtt 6300
ccgaccctgc?cgcttaccgg?atacctgtcc?gcctttctcc?cttcgggaag?cgtggcgctt 6360
tctcatagct?cacgctgtag?gtatctcagt?tcggtgtagg?tcgttcgctc?caagctgggc 6420
tgtgtgcacg?aaccccccgt?tcagcccgac?cgctgcgcct?tatccggtaa?ctatcgtctt 6480
gagtccaacc?cggtaagaca?cgacttatcg?ccactggcag?cagccactgg?taacaggatt 6540
agcagagcga?ggtatgtagg?cggtgctaca?gagttcttga?agtggtggcc?taactacggc 6600
tacactagaa?ggacagtatt?tggtatctgc?gctctgctga?agccagttac?cttcggaaaa 6660
agagttggta?gctcttgatc?cggcaaacaa?accaccgctg?gtagcggtgg?tttttttgtt 6720
tgcaagcagc?agattacgcg?cagaaaaaaa?ggatctcaag?aagatccttt?gatcttttct 6780
acggggtctg?acgctcagtg?gaacgaaaac?tcacgttaag?ggattttggt?catgagatta 6840
tcaaaaagga?tcttcaccta?gatcctttta?aattaaaaat?gaagttttaa?atcaatctaa 6900
agtatatatg?agtaacctga?tcaggactct?tccttttcat?gaacaataaa?actgtctgct 6960
tacataaaca?gtaatacaag?gggtgttatg?agccatattc?aacgggaaac?gtcttgctct 7020
aggccgcgat?taaattccaa?catggatgct?gatttatatg?ggtataaatg?ggctcgcgat 7080
aatgtcgggc?aatcaggtgc?gacaatctat?cgattgtatg?ggaagcccga?tgcgccagag 7140
ttgtttctga?aacatggcaa?aggtagcgtt?gccaatgatg?ttacagatga?gatggtcaga 7200
ctaaactggc?tgacggaatt?tatgcctctt?ccgaccatca?agcattttat?ccgtactcct 7260
gatgatgcat?ggttactcac?cactgcgatc?cccgggaaaa?cagcattcca?ggtattagaa 7320
gaatatcctg?attcaggtga?aaatattgtt?gatgcgctgg?cagtgttcct?gcgccggttg 7380
cattcgattc?ctgtttgtaa?ttgtcctttt?aacagcgatc?gcgtatttcg?tctcgctcag 7440
gcgcaatcac?gaatgaataa?cggtttggtt?gatgcgagtg?attttgatga?cgagcgtaat 7500
ggctggcctg?ttgaacaagt?ctggaaagaa?atgcataaac?ttttgccatt?ctcaccggat 7560
tcagtcgtca?ctcatggtga?tttctcactt?gataacctta?tttttgacga?ggggaaatta 7620
ataggttgta?ttgatgttgg?acgagtcgga?atcgcagacc?gataccagga?tcttgccatc 7680
ctatggaact?gcctcggtga?gttttctcct?tcattacaga?aacggctttt?tcaaaaatat 7740
ggtattgata?atcctgatat?gaataaattg?cagtttcatt?tgatgctcga?tgagtttttc 7800
taagaattcg?cgcaattaac?cctcactaaa?gggaacaaaa?gctgggtacc?gggccc 7856
<210>79
<211>8127
<212>DNA
＜213〉artificial sequence
<220>
<223>pCAH/SINd1/hlacZ
<400>79
gttgacattg?attattgact?agttattaat?agtaatcaat?tacggggtca?ttagttcata 60
gcccatatat?ggagttccgc?gttacataac?ttacggtaaa?tggcccgcct?ggctgaccgc 120
ccaacgaccc?ccgcccattg?acgtcaataa?tgacgtatgt?tcctatagta?acgccaatag 180
ggactttcca?ttgacgtcaa?tgggtggagt?atttacggta?aactgcccac?ttggcagtac 240
atcaagtgta?tcatatgcca?agtacgcccc?ctattgacgt?caatgacggt?aaatggcccg 300
cctggcatta?tgcccagtac?atgaccttat?gggactttcc?tacttggcag?tacatctacg 360
tattagtcat?cgctattacc?atggtgatgc?ggttttggca?gtacatcaat?gggcgtggat 420
agcggtttga?ctcacgggga?tttccaagtc?tccaccccat?tgacgtcaat?gggagtttgt 480
tttggcacca?aaatcaacgg?gactttccaa?aatgtcgtaa?caactccgcc?ccattgacgc 540
aaatgggcgg?taggcgtgta?cggtgggagg?tctatataag?cagagctcaa?gcttgctgct 600
tgcacttcag?agttctagga?gagtccctcc?tagtctctcc?tctccgagga?ggtaccgaga 660
cctcaaaata?aaggagtgat?tgccttactg?ccgagtggag?agtgattact?gagcggccgg 720
tgtatcggga?gtcgtccctt?aatctgtgca?ataccagagc?ggctctcgca?gctggcgccc 780
aacgtggggc?ccgaggagaa?gaaaagaaag?cggccctgag?aactcggctt?ctgaaaaaga 840
ggaagaggac?aagttgctat?agcaacaaga?gagaagaagt?agagcaaagg?tccagtggct 900
cggaaaaaga?ggaactgaaa?cttcggggac?gcctgaagga?gtaaggtaag?tgactctgct 960
gtacgcgggg?cgaggcagag?gtttccttct?aaattgaaag?agaagtgttg?ctgcgagagg 1020
tcttggtggt?cgagaatcct?gtacaaaaaa?aaggagggat?ctcggtcagg?accaggaccc 1080
ctgggagtaa?tacaacagca?acaccgtaag?aaaatccgcc?taggtgagtc?tagatagaga 1140
ctaggcgagg?caagtctccg?gggggaaaag?agattatcct?gagctcgaaa?aatgtatcaa 1200
gcatgcatgc?aagataaaag?ttcgactcag?aggggagcac?ttgacagaag?gaaattgttt 1260
atggtgcctt?aaaacattag?attacatgtt?tgaggaccat?aaagaggaac?cttggacaaa 1320
agtaaaattt?aggacaatat?ggcagaaggt?gaagaatcta?actcctgagg?agagtaacaa 1380
aaaagacttt?atgtctttgc?aggccacatt?agcgggtcta?atgtgttgcc?aaatggggat 1440
gagaccgggc?tgcaggaatt?cgattctaga?ggtgatagaa?atgccagaaa?actatgcaaa 1500
aacaagaatc?ataaacagga?aaaaaagaga?actcagccac?aagaggaaga?agagaggcgt 1560
tggcttggtc?attatgctag?ttatcatggc?aatagtagct?gccgcagggg?cttctctggg 1620
agtcgcaaac?gcgattcagc?agtcttacac?taaggcagct?gtccagaccc?ttgctaatgc 1680
aactgctgca?cagcaggatg?tgttagaggc?aacctatgcc?atggtacagc?atgtggctaa 1740
aggcgtacga?atcttggaag?ctcgagtggc?tcgagtggaa?gctatcacag?atagaataat 1800
gctataccaa?gaattggatt?gttggcacta?ggatccatca?gccaccatta?acgcttacaa 1860
tttacgcgcg?cgttgacatt?gattattgac?tagttattaa?tagtaatcaa?ttacggggtc 1920
attagttcat?agcccatata?tggagttccg?cgttacataa?cttacggtaa?atggcccgcc 1980
tggctgaccg?cccaacgacc?cccgcccatt?gacgtcaata?atgacgtatg?ttcccatagt 2040
aacgccaata?gggactttcc?attgacgtca?atgggtggac?tatttacggt?aaactgccca 2100
cttggcagta?catcaagtgt?atcatatgcc?aagtacgccc?cctattgacg?tcaatgacgg 2160
taaatggccc?gcctggcatt?atgcccagta?catgacctta?tgggactttc?ctacttggca 2220
gtacatctac?gtattagtca?tcgctattac?catggtgatg?cggttttggc?agtacatcaa 2280
tgggcgtgga?tagcggtttg?actcacgggg?atttccaagt?ctccacccca?ttgacgtcaa 2340
tgggagtttg?ttttggcacc?aaaatcaacg?ggactttcca?aaatgtcgta?acaactccgc 2400
cccattgacg?caaatgggcg?gtaggcgtgt?acggtgggag?gtctatataa?gcagagctct 2460
ctggctaact?agagaaccca?ctgcttactg?gcttatcgaa?attaatacga?ctcactatag 2520
ggagacccaa?gctttaagct?taccatgggg?ggttctcatc?atcatcatca?tcatggtatg 2580
gcatgactgg?tggacagcaa?atgggtcggg?atctgtacga?cgatgacgat?aaggtaccta 2640
aggatcagct?tggagttgat?cccgtcgttt?tacaacgtcg?tgactgggaa?aaccctggcg 2700
ttacccaact?taatcgcctt?gcagcacatc?cccctttcgc?cagctggcgt?aatagcgaag 2760
aggcccgcac?cgatcgccct?tcccaacagt?tgcgcagcct?gaatggcgaa?tggcgctttg 2820
cctggtttcc?ggcaccagaa?gcggtgccgg?aaagctggct?ggagtgcgat?cttcctgagg 2880
ccgatactgt?cgtcgtcccc?tcaaactggc?agatgcacgg?ttacgatgcg?cccatctaca 2940
ccaacgtaac?ctatcccatt?acggtcaatc?cgccgtttgt?tcccacggag?aatccgacgg 3000
gttgttactc?gctcacattt?aatgttgatg?aaagctggct?acaggaaggc?cagacgcgaa 3060
ttatttttga?tggcgttaac?tcggcgtttc?atctgtggtg?caacgggcgc?tgggtcggtt 3120
acggccagga?cagtcgtttg?ccgtctgaat?ttgacctgag?cgcattttta?cgcgccggag 3180
aaaaccgcct?cgcggtgatg?gtgctgcgtt?ggagtgacgg?cagttatctg?gaagatcagg 3240
atatgtggcg?gatgagcggc?attttccgtg?acgtctcgtt?gctgcataaa?ccgactacac 3300
aaatcagcga?tttccatgtt?gccactcgct?ttaatgatga?tttcagccgc?gctgtactgg 3360
aggctgaagt?tcagatgtgc?ggcgagttgc?gtgactacct?acgggtaaca?gtttctttat 3420
ggcagggtga?aacgcaggtc?gccagcggca?ccgcgccttt?cggcggtgaa?attatcgatg 3480
agcgtggtgg?ttatgccgat?cgcgtcacac?tacgtctgaa?cgtcgaaaac?ccgaaactgt 3540
ggagcgccga?aatcccgaat?ctctatcgtg?cggtggttga?actgcacacc?gccgacggca 3600
cgctgattga?agcagaagcc?tgcgatgtcg?gtttccgcga?ggtgcggatt?gaaaatggtc 3660
tgctgctgct?gaacggcaag?ccgttgctga?ttcgaggcgt?taaccgtcac?gagcatcatc 3720
ctctgcatgg?tcaggtcatg?gatgagcaga?cgatggtgca?ggatatcctg?ctgatgaagc 3780
agaacaactt?taacgccgtg?cgctgttcgc?attatccgaa?ccatccgctg?tggtacacgc 3840
tgtgcgaccg?ctacggcctg?tatgtggtgg?atgaagccaa?tattgaaacc?cacggcatgg 3900
tgccaatgaa?tcgtctgacc?gatgatccgc?gctggctacc?ggcgatgagc?gaacgcgtaa 3960
cgcgaatggt?gcagcgcgat?cgtaatcacc?cgagtgtgat?catctggtcg?ctggggaatg 4020
aatcaggcca?cggcgctaat?cacgacgcgc?tgtatcgctg?gatcaaatct?gtcgatcctt 4080
cccgcccggt?gcagtatgaa?ggcggcggag?ccgacaccac?ggccaccgat?attatttgcc 4140
cgatgtacgc?gcgcgtggat?gaagaccagc?ccttcccggc?tgtgccgaaa?tggtccatca 4200
aaaaatggct?ttcgctacct?ggagagacgc?gcccgctgat?cctttgcgaa?tacgcccacg 4260
cgatgggtaa?cagtcttggc?ggtttcgcta?aatactggca?ggcgtttcgt?cagtatcccc 4320
gtttacaggg?cggcttcgtc?tgggactggg?tggatcagtc?gctgattaaa?tatgatgaaa 4380
acggcaaccc?gtggtcggct?tacggcggtg?attttggcga?tacgccgaac?gatcgccagt 4440
tctgtatgaa?cggtctggtc?tttgccgacc?gcacgccgca?tccagcgctg?acggaagcaa 4500
aacaccagca?gcagtttttc?cagttccgtt?tatccgggca?aaccatcgaa?gtgaccagcg 4560
aatacctgtt?ccgtcatagc?gataacgagc?tcctgcactg?gatggtggcg?ctggatggta 4620
agccgctggc?aagcggtgaa?gtgcctctgg?atgtcgctcc?acaaggtaaa?cagttgattg 4680
aactgcctga?actaccgcag?ccggagagcg?ccgggcaact?ctggctcaca?gtacgcgtag 4740
tgcaaccgaa?cgcgaccgca?tggtcagaag?ccgggcacat?cagcgcctgg?cagcagtggc 4800
gtctggcgga?aaacctcagt?gtgacgctcc?ccgccgcgtc?ccacgccatc?ccgcatctga 4860
ccaccagcga?aatggatttt?tgcatcgagc?tgggtaataa?gcgttggcaa?tttaaccgcc 4920
agtcaggctt?tctttcacag?atgtggattg?gcgataaaaa?acaactgctg?acgccgctgc 4980
gcgatcagtt?cacccgtgca?ccgctggata?acgacattgg?cgtaagtgaa?gcgacccgca 5040
ttgaccctaa?cgcctgggtc?gaacgctgga?aggcggcggg?ccattaccag?gccgaagcag 5100
cgttgttgca?gtgcacggca?gatacacttg?ctgatgcggt?gctgattacg?accgctcacg 5160
cgtggcagca?tcaggggaaa?accttattta?tcagccggaa?aacctaccgg?attgatggta 5220
gtggtcaaat?ggcgattacc?gttgatgttg?aagtggcgag?cgatacaccg?catccggcgc 5280
ggattggcct?gaactgccag?ctggcgcagg?tagcagagcg?ggtaaactgg?ctcggattag 5340
ggccgcaaga?aaactatccc?gaccgcctta?ctgccgcctg?ttttgaccgc?tgggatctgc 5400
cattgtcaga?catgtatacc?ccgtacgtct?tcccgagcga?aaacggtctg?cgctgcggga 5460
cgcgcgaatt?gaattatggc?ccacaccagt?ggcgcggcga?cttccagttc?aacatcagcc 5520
gctacagtca?acagcaactg?atggaaacca?gccatcgcca?tctgctgcac?gcggaagaag 5580
gcacatggct?gaatatcgac?ggtttccata?tggggattgg?tggcgacgac?tcctggagcc 5640
cgtcagtatc?ggcggaatta?cagctgagcg?ccggtcgcta?ccattaccag?ttggtctggt 5700
gtcaaaaata?ataaagccga?attctgcaga?tatccagcac?agtggcggcc?gctagcacaa 5760
aaataaaaaa?agaaagggtg?actgtgagac?atgggctaaa?gaggagcggc?cgctcgagtc 5820
tagaactagt?ggatcagctt?tgctgcttgc?acttcagagt?tctaggagag?tccctcctag 5880
tctctcctct?ccgaggaggt?accgagacct?caaaataaag?gagtgattgc?cttactgccg 5940
agtggagagt?gattactgag?cggccggtgt?atcgggagtc?gtcccttaat?ctgtgcaata 6000
ccagagcggc?tctcgcagcc?gacctcgagg?gggggcccta?ttctatagtg?tcacctaaat 6060
gctagagctc?gctgatcagc?ctcgactgtg?ccttctagtt?gccagccatc?tgttgtttgc 6120
ccctcccccg?tgccttcctt?gaccctggaa?ggtgccactc?ccactgtcct?ttcctaataa 6180
aatgaggaaa?ttgcatcgca?ttgtctgagt?aggtgtcatt?ctattctggg?gggtggggtg 6240
gggcaggaca?gcaaggggga?ggattgggaa?gacaatagca?ggcatgctgg?ggatgcggtg 6300
ggctctatgg?cttctgaggc?ggaaagaacc?agtggcggta?atacggttat?ccacagaatc 6360
aggggataac?gcaggaaaga?acatgtgagc?aaaaggccag?caaaaggcca?ggaaccgtaa 6420
aaaggccgcg?ttgctggcgt?ttttccatag?gctccgcccc?cctgacgagc?atcacaaaaa 6480
tcgacgctca?agtcagaggt?ggcgaaaccc?gacaggacta?taaagatacc?aggcgtttcc 6540
ccctggaagc?tccctcgtgc?gctctcctgt?tccgaccctg?ccgcttaccg?gatacctgtc 6600
cgcctttctc?ccttcgggaa?gcgtggcgct?ttctcatagc?tcacgctgta?ggtatctcag 6660
ttcggtgtag?gtcgttcgct?ccaagctggg?ctgtgtgcac?gaaccccccg?ttcagcccga 6720
ccgctgcgcc?ttatccggta?actatcgtct?tgagtccaac?ccggtaagac?acgacttatc 6780
gccactggca?gcagccactg?gtaacaggat?tagcagagcg?aggtatgtag?gcggtgctac 6840
agagttcttg?aagtggtggc?ctaactacgg?ctacactaga?aggacagtat?ttggtatctg 6900
cgctctgctg?aagccagtta?ccttcggaaa?aagagttggt?agctcttgat?ccggcaaaca 6960
aaccaccgct?ggtagcggtg?gtttttttgt?ttgcaagcag?cagattacgc?gcagaaaaaa 7020
aggatctcaa?gaagatcctt?tgatcttttc?tacggggtct?gacgctcagt?ggaacgaaaa 7080
ctcacgttaa?gggattttgg?tcatgagatt?atcaaaaagg?atcttcacct?agatcctttt 7140
aaattaaaaa?tgaagtttta?aatcaatcta?aagtatatat?gagtaacctg?atcaggactc 7200
ttccttttca?tgaacaataa?aactgtctgc?ttacataaac?agtaatacaa?ggggtgttat 7260
gagccatatt?caacgggaaa?cgtcttgctc?taggccgcga?ttaaattcca?acatggatgc 7320
tgatttatat?gggtataaat?gggctcgcga?taatgtcggg?caatcaggtg?cgacaatcta 7380
tcgattgtat?gggaagcccg?atgcgccaga?gttgtttctg?aaacatggca?aaggtagcgt 7440
tgccaatgat?gttacagatg?agatggtcag?actaaactgg?ctgacggaat?ttatgcctct 7500
tccgaccatc?aagcatttta?tccgtactcc?tgatgatgca?tggttactca?ccactgcgat 7560
ccccgggaaa?acagcattcc?aggtattaga?agaatatcct?gattcaggtg?aaaatattgt 7620
tgatgcgctg?gcagtgttcc?tgcgccggtt?gcattcgatt?cctgtttgta?attgtccttt 7680
taacagcgat?cgcgtatttc?gtctcgctca?ggcgcaatca?cgaatgaata?acggtttggt 7740
tgatgcgagt?gattttgatg?acgagcgtaa?tggctggcct?gttgaacaag?tctggaaaga 7800
aatgcataaa?cttttgccat?tctcaccgga?ttcagtcgtc?actcatggtg?atttctcact 7860
tgataacctt?atttttgacg?aggggaaatt?aataggttgt?attgatgttg?gacgagtcgg 7920
aatcgcagac?cgataccagg?atcttgccat?cctatggaac?tgcctcggtg?agttttctcc 7980
ttcattacag?aaacggcttt?ttcaaaaata?tggtattgat?aatcctgata?tgaataaatt 8040
gcagtttcat?ttgatgctcg?atgagttttt?ctaagaattc?gcgcaattaa?ccctcactaa 8100
agggaacaaa?agctgggtac?cgggccc 8127

Claims (78)

1. transfer vector, it comprises:
(a) arthritis-Encephalitis virus (CAEV) packaging sequence, it is made up of following substantially: (i) non-translational region between CAEV5 ' LTR and the CAEVgag-encoding sequence and the Nucleotide 1 of CAEV gag-encoding sequence that (ii) is connected to described non-translational region 3 ' end are to X, and wherein X is less than 613; And
(b) the required cis-acting elements of polyadenylic acidization, rna transport, reverse transcription and integration, it is connected with described packaging sequence operability.
2. the transfer vector of claim 1, wherein X is selected from 60,100,125,150,175,200,225,250,275,300,325,350,375,400,425,450,475,500,525,550,575 and 600.
3. the transfer vector of claim 1, wherein X is selected from:
(a) X is greater than 25 and less than 600,
(b) X is greater than 25 and less than 500,
(c) X is greater than 25 and less than 400,
(d) X is greater than 25 and less than 300,
(e) X is greater than 25 and less than 200,
(f) X is greater than 50 and less than 600,
(g) X is greater than 50 and less than 500,
(h) X is greater than 50 and less than 400,
(i) X is greater than 50 and less than 300,
(j) X is greater than 50 and less than 200,
(k) X is greater than 75 and less than 600,
(l) X is greater than 75 and less than 500,
(m) X is greater than 75 and less than 400,
(n) X is greater than 75 and less than 300,
(o) X is greater than 75 and less than 200,
(p) X is greater than 100 and less than 600,
(q) X is greater than 100 and less than 500,
(r) X is greater than 100 and less than 400,
(s) X is greater than 100 and less than 300,
(t) X is greater than 100 and less than 200,
(u) X is greater than 125 and less than 600,
(v) X is greater than 125 and less than 500,
(w) X is greater than 125 and less than 400,
(x) X is greater than 125 and less than 300,
(y) X is greater than 125 and less than 200,
(z) X is greater than 150 and less than 600,
(aa) X is greater than 150 and less than 500,
(bb) X is greater than 150 and less than 400,
(cc) X is greater than 150 and less than 300,
(dd) X is greater than 150 and less than 200,
(ee) X is greater than 200 and less than 600,
(ff) X is greater than 200 and less than 500,
(gg) X is greater than 200 and less than 400,
(hh) X is greater than 200 and less than 300,
(ii) X is greater than 200 and less than 200,
(jj) X is greater than 250 and less than 600,
(kk) X is greater than 250 and less than 500,
(ll) X greater than 250 and less than 400 and
(mm) X is greater than 250 and less than 300.
4. the transfer vector of claim 1, wherein X is greater than 40 and less than 613.
5. the transfer vector of claim 1, wherein X is greater than 57 and less than 613.
6. the transfer vector of claim 1, wherein X is about 327.
7. the transfer vector of claim 1, wherein the initiator codon of gag-encoding sequence is suddenlyd change to prevent the proteic translation of gag.
8. the transfer vector of claim 7, wherein said initiator codon is mutated into TAG.
9. the transfer vector of claim 7, the ATG codon that wherein is positioned at the gag-encoding sequence at X base pair place, initiator codon ATG downstream is suddenlyd change to prevent the proteic translation of gag, and wherein X is less than 30.
10. the transfer vector of claim 9, wherein X is about 21.
11. the transfer vector of claim 1, it also comprises the RRE district.
12. the transfer vector of claim 1, it also comprises CAEV3 ' LTR, and wherein the U3 district is deleted.
13. the transfer vector of claim 1, it also comprises allogeneic promoter.
14. the transfer vector of claim 13, wherein allogeneic promoter is the main immediate early promoter (HCMV MIEP) of human cytomegalic inclusion disease virus.
15. the transfer vector of claim 1, wherein said carrier have the pCAH/SINd1 structure shown in Fig. 3 C.
16. the transfer vector of claim 1, it also comprises transcribes box.
17. the transfer vector of claim 16, the wherein said box of transcribing comprises the polynucleotide interested that operability is connected to allogeneic promoter.
18. carrier system, it comprises the transfer vector and the package carrier system of claim 1, wherein said package carrier system comprises: contain first polynucleotide of CAEV gag-pol-encoding sequence and RRE, and the mat woven of fine bamboo strips two polynucleotides that contain the peplos encoding sequence.
19. also comprising, the carrier system of claim 18, wherein said transfer vector transcribe box.
20. the carrier system of claim 19, the wherein said box of transcribing comprises the polynucleotide interested that operability is connected to allogeneic promoter.
21. the carrier system of claim 18, wherein said peplos-encoding sequence right and wrong CAEV coating-encoding sequence.
22. the carrier system of claim 21, wherein said non-CAEV coating-encoding sequence is VSV-G-encoding sequence or GaL-V encoding sequence.
23. the carrier system of claim 22, wherein said coating-encoding sequence are the VSV-G-encoding sequences.
24. the carrier system of claim 22, wherein said coating-encoding sequence are the GaL-V encoding sequences.
25. the carrier system of claim 18, wherein said carrier system also comprise the 3rd polymerized nucleoside acid sequence that contains the rev-encoding sequence.
26. the carrier system of claim 18 or 25, wherein said carrier system also comprise the 4th polymerized nucleoside acid sequence that contains the vif-encoding sequence.
27. also comprising the allos that operability is connected to described CAEV gag-pol-encoding sequence, claim 18,25 or 26 each carrier systems, wherein said first polynucleotide regulate sequence.
28. also comprising the allos that operability is connected to described peplos-encoding sequence, claim 18,21 or 22 each carrier systems, wherein said second polynucleotide regulate sequence.
29. the carrier system of claim 18, wherein said first polynucleotide also comprises the allos adjusting sequence that operability is connected to described CAEV gag-pol-encoding sequence, and second polynucleotide also comprises the allos adjusting sequence that operability is connected to described peplos-encoding sequence.
30. the carrier system of claim 25, wherein said the 3rd polynucleotide also comprise the allos adjusting sequence that operability is connected to described rev-encoding sequence.
31. the carrier system of claim 26, wherein said the 4th polynucleotide also comprise the allos adjusting sequence that operability is connected to described vif-encoding sequence.
32. claim 27,28,29,30 or 31 carrier system, it is promotor that wherein said allos is regulated sequence.
33. the carrier system of claim 18, wherein said package carrier system disappearance has the CAEV packaging sequence of function.
34. the carrier system of claim 18, the CAEV genome 5 ' end between wherein said package carrier system's disappearance donor splicing site and the gag initiator codon.
35. the carrier system of claim 18, it comprises first carrier that contains first polynucleotide and second carrier that contains second polynucleotide.
36. the carrier system of claim 25, it comprises first carrier that contains described first polynucleotide, the 3rd carrier that contains second carrier of described second polynucleotide and contain described the 3rd polynucleotide.
37. the carrier system of claim 26, the 4th carrier that it comprises first carrier that contains described first polynucleotide, second carrier that contains described second polynucleotide, contains the 3rd carrier of described the 3rd polynucleotide and contain described the 4th polynucleotide.
38. the carrier system of claim 25, it comprises first carrier that contains described first polynucleotide and described the 3rd polynucleotide and second carrier that contains described second polynucleotide.
39. the carrier system of claim 26, it comprises first carrier that contains described first polynucleotide and described the 4th polynucleotide and second carrier that contains described second polynucleotide.
40. the carrier system of claim 32, wherein said CAEV gag-pol-encoding sequence operability is connected to MCMV MIEP promotor.
41. the carrier system of claim 40, wherein said first carrier has the pMGP/RRE structure shown in Fig. 2 A.
Be connected to the VSV-G-encoding sequence of HCMV MIEP promotor 42. the carrier system of claim 35, wherein said peplos-encoding sequence are operability, and wherein said second carrier also comprises the beta-globin intron.
43. the carrier system of claim 42, wherein said second carrier has the pHGVSV-G structure shown in Fig. 6 A.
Be connected to the GaLV-encoding sequence of MCMV MIEP promotor 44. the carrier system of claim 35, wherein said peplos-encoding sequence are operability, and wherein said second carrier also comprises eucaryon elongation factor-1 alpha intron.
45. the carrier system of claim 44, wherein said second carrier has the pMYKEF-1/env structure shown in Fig. 6 B.
46. the carrier system of claim 36, wherein said the 3rd carrier has pHYK/rev structure shown in Figure 5.
47. the carrier system of claim 37, wherein said the 4th carrier has pHYK/vif structure shown in Figure 4.
48. produce the method based on the carrier granule of CAEV, it comprises:
(a) the carrier system transfectional cell of usefulness claim 20;
(b) hatch described cell under the lentiviral vectors particulate condition of be fit to producing based on CAEV, wherein carrier granule has infectious and transduction ability and be replication defect type; With
(c) reclaim described carrier granule.
49. the carrier granule that the method for claim 48 is produced.
50. comprise the carrier granule of claim 49 and the composition of mediator.
51. comprise the medicine box of each carrier system among the claim 18-47.
52. polypeptide delivery is gone into the method for mammalian cell, and it comprises makes described mammalian cell contact with the carrier granule of claim 49.
53. the method for claim 52, wherein said cell separated from Mammals before the contact of cell and carrier granule.
54. the method for claim 52, wherein said mammalian cell is a somatoblast.
55. the method for claim 52, wherein said mammalian cell is a Unseparated Cell.
56. the method for claim 52, wherein said mammalian cell are the CD34+ stem cells.
57. polypeptide delivery is gone into vertebrate method, and it comprises carrier granule from claim 49 to described vertebrates that use.
58. comprise the medicine box of each transfer vector among the claim 1-17.
59. each carrier system among the claim 18-47, it also comprises the cell that contains described first polynucleotide.
60. each carrier system in the claim 28,30,32,36 or 38, it also comprises and contains described first and the cell of the 3rd polynucleotide.
61. each carrier system among claim 26-28, the 31-32,37,39,41,43 or 45, it also comprises and contains described first and the cell of the 4th polynucleotide.
62. each carrier system among claim 26-28, the 31-32,37,39,41,43 or 45, it also comprises the cell that contains described first, third and fourth polynucleotide.
63. each carrier system among claim 18-24,26,29, the 33-35,40,42,44 or 46, it also comprises the cell that contains described first and second polynucleotides.
64. each carrier system among the claim 25-28,30,32,36 or 38, it also comprises the cell that contains described first, second and the 3rd polynucleotide.
65. each carrier system among claim 26-28, the 31-32,37,39,41,43 or 45, it also comprises the cell that contains described first, second and the 4th polynucleotide.
66. each carrier system among claim 26-28, the 31-32,37,39,41,43 or 45, it also comprises and contains described first, second, third and the cell of the 4th polynucleotide.
67. each carrier system among the claim 35-47, it also comprises the cell that contains described first and second carriers.
68. each carrier system among the claim 36-46, it also comprises the cell that contains described first, second and the 3rd carrier.
69. each carrier system among the claim 36-47, it also comprises and contains described first, second, third and the cell of the 4th carrier.
70. each carrier system among the claim 35-47, it also comprises the cell that contains described first carrier.
71. produce the method based on the carrier granule of CAEV, it comprises:
(a) preparation comprises the cell of CAEV gag-pol-encoding sequence and RRE;
(b) the described cell of transfer vector transfection of usefulness claim 17;
(c) hatch described cell under the lentiviral vectors particulate condition of be fit to producing based on CAEV, wherein carrier granule has infectious and transduction ability and be replication defect type; With
(d) reclaim described carrier granule.
72. contain the carrier of CAEV packaging sequence, wherein said CAEV packaging sequence is made up of following substantially: (a) non-translational region between CAEV 5 ' LTR and the CAEV gag-encoding sequence, and the Nucleotide 1 of CAEV gag-encoding sequence that (b) is connected to described non-translational region 3 ' end is to X, and wherein X is less than 613.
73. the transfer vector of claim 15, wherein said carrier and SEQ ID NO:68 have at least 70% consistence.
74. the carrier system of claim 41, wherein said first carrier and SEQ ID NO:77 have at least 70% consistence.
75. the carrier system of claim 43, wherein said second carrier and SEQ ID NO:74 have at least 70% consistence.
76. the carrier system of claim 45, wherein said second carrier and SEQ ID NO:72 have at least 70% consistence.
77. the carrier system of claim 46, wherein said the 3rd carrier and SEQ ID NO:75 have at least 70% consistence.
78. the carrier system of claim 47, wherein said the 4th carrier and SEQ ID NO:76 have at least 70% consistence.
CNA2004800439386A 2004-09-07 2004-09-07 CAEV-based vector system Pending CN101014710A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/KR2004/002274 WO2006028302A1 (en) 2004-09-07 2004-09-07 Caev-based vector systems

Publications (1)

Publication Number Publication Date
CN101014710A true CN101014710A (en) 2007-08-08

Family

ID=36036565

Family Applications (1)

Application Number Title Priority Date Filing Date
CNA2004800439386A Pending CN101014710A (en) 2004-09-07 2004-09-07 CAEV-based vector system

Country Status (4)

Country Link
EP (1) EP1799830A4 (en)
JP (1) JP2008512110A (en)
CN (1) CN101014710A (en)
WO (1) WO2006028302A1 (en)

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2328491A1 (en) * 1998-05-22 1999-12-02 Oxford Biomedica (Uk) Limited Retroviral delivery system
KR20020008406A (en) * 1999-05-21 2002-01-30 찰스 굿맨 Method for selecting improved vectors

Also Published As

Publication number Publication date
WO2006028302A1 (en) 2006-03-16
JP2008512110A (en) 2008-04-24
EP1799830A4 (en) 2009-05-13
EP1799830A1 (en) 2007-06-27

Similar Documents

Publication Publication Date Title
AU2020260485B2 (en) Gene therapies for lysosomal disorders
AU2020260476B2 (en) Gene therapies for lysosomal disorders
KR20230091894A (en) Systems, methods, and compositions for site-specific genetic engineering using programmable addition via site-specific targeting elements (PASTE)
AU2016317936A1 (en) Recombinant vectors comprising 2A peptide
KR20200078512A (en) Gene therapy for lysosomal disorders
KR20210150486A (en) Gene therapy for lysosomal disorders
KR20230066360A (en) Gene Therapy for Neurodegenerative Disorders
AU2019325609A1 (en) Vector production in serum free media
KR20220002910A (en) Triple helix terminator for efficient RNA trans-splicing
KR20230010231A (en) Vectors and methods for in vivo transduction
KR20070114761A (en) Remedy for disease associated with apoptotic degeneration in ocular cell tissue with the use of siv-pedf vector
KR20200107990A (en) Constructs containing nerve survival factors and uses thereof
CN101160139A (en) Therapeutic agent for disease with apoptotic degeneration in eye tissue cell containing PEDF and FGF2
KR100884214B1 (en) Caev-based vector systems
KR20230117327A (en) An expression vector comprising a soluble alkaline phosphatase construct and a polynucleotide encoding the soluble alkaline phosphatase construct.
CN101014710A (en) CAEV-based vector system
CN114645066B (en) Nucleic acid construct for gene therapy of AIDS
KR20210150487A (en) Gene Therapy for Lysosomal Disorders
CN113677800A (en) Recombinant vector comprising a gene for a binding domain and a secretable peptide
TW202233830A (en) Compositions and methods for the treatment of cancer using next generation engineered t cell therapy
AU2022337765A1 (en) Gene sequence construct for gene therapy of human immunodeficiency virus infection
AU2022338817A1 (en) Gene sequence construct for gene therapy for hiv infection

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C02 Deemed withdrawal of patent application after publication (patent law 2001)
WD01 Invention patent application deemed withdrawn after publication

Open date: 20070808