CN101003787A - Degradation bacteria capable of degrading lasting rudimental herbicide of clomazone in high efficiency, and cultivation method - Google Patents
Degradation bacteria capable of degrading lasting rudimental herbicide of clomazone in high efficiency, and cultivation method Download PDFInfo
- Publication number
- CN101003787A CN101003787A CN 200710071616 CN200710071616A CN101003787A CN 101003787 A CN101003787 A CN 101003787A CN 200710071616 CN200710071616 CN 200710071616 CN 200710071616 A CN200710071616 A CN 200710071616A CN 101003787 A CN101003787 A CN 101003787A
- Authority
- CN
- China
- Prior art keywords
- clomazone
- herbicide
- degrading
- high efficiency
- degradation bacteria
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Abstract
This invention provides to a method for culturing degradation bacteria of long-residual herbicide clomazone. The method comprises: uniformly mixing soil samples with long-term application of long-residual herbicide clomazone, weighing 10 g, adding into 200 mg/L clomazone-rich culture solution 50 Ml, culturing in a shaking table of 120 rpm at 30 deg.C for 2 months, adding fresh culture solution 10 mL per week, adding clomazone concentration for each time (200, 400, 800, 1600, 2000, 3000, 4000 and 5000 mg/L, respectively), culturing for 1 week after the clomazone concentration in the culture solution is up to 5000 mg/L, coating on the inorganic salt culture medium containing 200 mg/L clomazone, picking up single colony, streaking for purifying, and storing purified bacteria. The bacteria can degrade long-residual herbicide clomazone, thus can be used for biological repairing of polluted soil.
Description
(1) technical field
What the present invention relates to is a kind of biotechnological formulation, and the present invention also relates to a kind of is the preparation method of thing preparation, the invention still further relates to a kind of application method of biotechnological formulation.
(2) background technology
The use of chemical pesticide has promoted the development of agricultural modernization greatly, has retrieved the loss that causes because of reasons such as crop smotherings in a large number for agriculture production, has played crucial effects in the modernization that advances agriculture production.But agricultural chemicals, must exert an influence to the ecosystem after they enter the ecosystem as the environment foreign matter.
High reactivity, long residual effect weedicide, as G-30027, green sulphur is grand, chlorimuronethyl, imazethapyr, ethametsulfuron clomazone etc., obtained in China the Northeast in recent years using widely, immeasurable effect has been played in the increasing both production and income of agricultural, their longevities of residure in soil reach 2-3, and use continuously in soil and can accumulate, this makes it in crop rotation, to late stubble sensitive crop (vegetables, paddy rice, beet, rape etc.) easily cause serious poisoning, cause heavy losses to agriculture production, even total crop failure, this fact has had a strong impact on China particularly adjustment and the expanding economy of Heilongjiang Province's agricultural planting structure, and pesticide residue directly have influence on the production of green food and the competitive power that agricultural-food enter the world market.In addition, lasting rudimental herbicide brings very big difficulty and challenge to work of conceding the land to forestry, and the residual surviving rate that seriously reduces seeds of medicament has caused huge waste and loss, has seriously hindered the realization of the ecological target of China.
The approach that solves lasting rudimental herbicide harm mainly contains: 1. develop the weedicide new variety to replace existing long residual herbicide, but it is because not enough to the degree of understanding of target enzyme, develop the pesticide new variety multi-billion dollar of will injecting capital into, so difficulty is very big in addition.2. turn to the seed selection of antiweed genetically modified crops kind, seek, find and isolate resistant gene, and then resistant gene is imported crop.Commercializations such as resistance glyphosate soybean, cotton, corn, wheat, rape and beet are only arranged at present, and about genetically modified product and food very big dispute in addition; And need to drop into a large amount of reasearch funds and manpower, experience long research process, far away apart from practical application.3. safe in utilization dose, this has and relates to being invested in the environment of another chemical substance, can cause secondary pollution problem.4. utilize the poisoning of endogenous plant growth regulator (Plant hormones regulators,gibberellins, indolylacetic acid, study on brassinolide and Kang Kai etc.) relieving herbicide, but can not play the effect that purifies soil and 5. utilize the microbiological deterioration that comes from soil, be only the real effective way that can eliminate the lasting rudimental herbicide pollution.Microbial source is in nature, back to nature again, efficient, safety, non-secondary pollution.Therefore, biological degradation is a main path of removing organic pollutant in the environment, is the main process of biological restoration, also is the practicable effective way that solves lasting rudimental herbicide harm at present.
(3) summary of the invention
But the object of the present invention is to provide a kind of degradation bacteria of degrading lasting rudimental herbicide of clomazone in high efficiency, the present invention also aims to provide a kind of cultural method of degradation bacteria.
The object of the present invention is achieved like this:
But the degradation bacteria of degrading lasting rudimental herbicide of clomazone in high efficiency provided by the invention is classification called after Achromobacter xylosoxidans wood sugar oxidation subspecies (Achromobacter xylosoxidans ss xylosoxidans); Have following characteristic: optimum growth temperature is 30 ℃, and optimal pH is 6.0-9.0, can be the sole carbon source growth Yi clomazone, and viable count just can reach and surpass 100,000,000/gram (milliliter) under common culture condition.
But the degradation bacteria of degrading lasting rudimental herbicide of clomazone in high efficiency of the present invention is to adopt such method to cultivate:
1, soil sample: the soil that picks up from the long-term application long residual herbicide;
2, the enrichment of thalline with separate
The pedotheque of long-term application long residual herbicide is mixed, take by weighing 10g and join and contain 200mgL
-1In the 50ml enrichment culture liquid of clomazone, place 30 ℃, 120rmin
-1Shaking table cultivate, add the 10ml fresh medium later on weekly, increase the concentration of clomazone at every turn, make that its concentration is respectively 200,400,800,1600,2000,3000,4000,5000mgL
-1, the enrichment culture time was respectively 2 months, and nutrient solution Zhong clomazone concentration reaches 5000mgL
-1After, adaptability is cultivated a week, with plate dilution method it is coated on and contains 200mgL
-1On the minimal medium of clomazone, picking list bacterium colony, the line purifying is preserved the single bacterium colony behind the purifying.
Composition and the proportioning such as the table 1 of described substratum (liquid):
The composition and the proportioning of table 1 substratum (liquid)
| The substratum title | Medium component |
| Enrichment culture liquid minimal medium | Extractum carnis: 3g, peptone: 5g, NaCl:5g, K 2HPO 4: 5g, distilled water: 1000ml, pH:7.0-7.2 KH 2PO 4：1.70g，K 2HPO 4：5.17g，(NH 4) 2SO 4: 2.6g, water: 1000ml., salts solution: 10ml, the PH:7.0-7.2 salts solution constitutes: MgSO 4：19.5g，MnSO 4：5g，FeSO 4：5g， CaCl 2: 0.3g, water: 1000ml |
But the evaluation of the degradation bacteria of efficient degradation long residual herbicide clomazone provided by the invention
But the morphological specificity of the degradation bacteria bacterial strain of 1 degrading lasting rudimental herbicide of clomazone in high efficiency
W
2Bacterial strain is rule in beef-protein medium, place 30 ℃ of constant incubators to cultivate 24h after, observe colony characteristics and be: smooth surface, moistening, protruding, the little spination in edge, planar growth, opaque, the substratum dark yellow.
But the degradation bacteria of degrading lasting rudimental herbicide of clomazone in high efficiency is seeded in the test tube that the beef extract-peptone nutrient solution is housed, after placing 30 ℃ of constant incubators to cultivate 24h, observe the nutrient solution status discovery, nutrient solution is cloudy state, there is not precipitation, the surface sterile film.
But the physiological and biochemical property of the degradation bacteria bacterial strain of 2 degrading lasting rudimental herbicide of clomazone in high efficiency
But the degradation bacteria bacterial strain of degrading lasting rudimental herbicide of clomazone in high efficiency is shaft-like, Gram-negative, and the catalase positive, oxidase negative can extensively utilize amino acid, organic acid, and concrete feature sees Table 2.
Table 2W
2The physiological and biochemical property of bacterial strain
| Project | The result | Project | The result |
| Gramstaining | Negative | Formic acid | + |
| Cell shape | Shaft-like | The D-galactonolactone | + |
| Catalase | + | The D-galacturonic acid | - |
| Oxydase | - | Propanedioic acid | +W |
| The a-cyclodextrin | - | Propionic acid | + |
| Dextrin | - | Succinic Acid | + |
| Glycogen | +W | Bromosuccinic acid | + |
| Tween40 | + | Succinamic acid | + |
| Tween80 | + | The D-L-Ala | + |
| N-acetylgalactosamine | - | The L-aspartic acid | + |
| The N-acetylglucosamine | - | L-L-glutamic acid | + |
| Ribitol | - | The glycyl aspartic acid | + |
| L-arabinose | + | Glycyl L-glutamic acid | + |
| The D-arabitol | - | The L-Histidine | + |
| The D-cellobiose | - | Oxyproline | - |
| The i-tetrahydroxybutane | - | The L-leucine | + |
| D-fructose | - | The L-ornithine | + |
| L-fructose | - | The L-phenylalanine | + |
| The D-semi-lactosi | - | The L-proline(Pro) | + |
| Gentiobiose | - | The D-Serine | - |
| A-D-glucose | + | The L-Serine | + |
| Maltose | - | The L-Threonine | +W |
| Methyl glucoside | - | Inosine | - |
| The D-raffinose | - | The urea glycosides | - |
| The D-rhamnosyl | - | Thymidine | - |
| The D-sorbyl alcohol | - | Phenyl-ethyl amine | + |
| Sucrose | - | Putrescine | - |
| Xylitol | - | The 2-monoethanolamine | - |
| Pyruvic Acid Methyl ester | + | 2, the 3-butyleneglycol | -W |
| Succinic Acid-methyl esters | + | Glycerine | - |
| Acetate | + | The D-glucose-1-phosphate | - |
| Citric acid | + | The D-Robison ester | - |
But according to physio-biochemical characteristics combining form feature the most at last the degradation bacteria bacterial strain of degrading lasting rudimental herbicide of clomazone in high efficiency range Achromobacter xylosoxidans wood sugar oxidation subspecies (Achromobacter xylosoxidansss xylosoxidans).
But the purposes of the degradation bacteria of efficient degradation long residual herbicide clomazone provided by the invention:
This bacterial strain degradable long residual herbicide clomazone can be used for being subjected to the biological restoration of this material Contaminated soil.
But the degradation bacteria W2 bacterial strain of 1 degrading lasting rudimental herbicide of clomazone in high efficiency is right in sterile soil
200 μ gkg
-1The Degradation of De clomazone:
But the degradation bacteria W2 bacterial strain of degrading lasting rudimental herbicide of clomazone in high efficiency is cultured to OD earlier in the beef extract-peptone liquid medium
650, inoculate 5mlkg respectively at=0.4 o'clock
-1And 20mlkg
-1Bacterial strain to content of dispersion be 200 μ gkg
-1In the soil of De clomazone, keeping the humidity of soil is 60% of field capacity, degraded situation such as Fig. 1 of results of regular determination clomazone.As can be seen from Figure 1, W
2Bacterial strain has had fast residual De clomazone in the soil after through about 20 days environmental adaptation and has utilized, and by the 90th day, W is arranged
2The processing of bacterial strain will be compared according to the 70% De clomazones nearly of degrading more.
But the degradation bacteria W2 bacterial strain of 2 degrading lasting rudimental herbicide of clomazone in high efficiency in sterile soil to 200 μ gkg
-1The Degradation of De clomazone:
But the degradation bacteria W2 bacterial strain of degrading lasting rudimental herbicide of clomazone in high efficiency is cultured to OD earlier in the beef extract-peptone liquid medium
650, inoculate 5mlkg respectively at=0.4 o'clock
-1And 20mlkg
-1Bacterial strain to content of dispersion be 200 μ gkg
-1In the soil of De clomazone, keeping the humidity of soil is 40% of field capacity, degraded situation such as Fig. 2 of results of regular determination clomazone.As can be seen from Figure 2, but the degradation bacteria bacterial strain of degrading lasting rudimental herbicide of clomazone in high efficiency is under 40% soil humidity of field capacity, the time that the condition that conforms needs is long slightly, utilize the time of clomazone to appear between the 30th day to the 70th day fast, during by the 90th day, the residual rate of soil Zhong clomazone also only is left about 20%.
But the degradation bacteria W2 bacterial strain of 3 degrading lasting rudimental herbicide of clomazone in high efficiency in natural soils to 500 μ gkg
-1The Degradation of De clomazone:
But the degradation bacteria W2 bacterial strain of degrading lasting rudimental herbicide of clomazone in high efficiency is cultured to OD earlier in the beef extract-peptone liquid medium
650, inoculate 5mlkg respectively at=0.4 o'clock
-1And 20mlkg
-1Bacterial strain to content of dispersion be 500 μ gkg
-1In the soil of De clomazone, keeping the humidity of soil is 60% of field capacity, degraded situation such as Fig. 3 of results of regular determination clomazone.As can be seen from Figure 3, the clomazone can be degraded slowly under field conditions (factors), during by the 90th day, degradation rate nearly reaches 40%, and but the speed general performance of degrading under the situation of the degradation bacteria bacterial strain that degrading lasting rudimental herbicide of clomazone in high efficiency is arranged the trend of quickening, and during by the 90th day, the residual rate of clomazone also only is left about 10% in the soil.
(4) description of drawings
But Fig. 1 be the degradation bacteria W2 bacterial strain of degrading lasting rudimental herbicide of clomazone in high efficiency in field capacity 60% sterile soil to 200 μ gkg
-1The degraded situation of De clomazone;
But Fig. 2 be the degradation bacteria W2 bacterial strain of degrading lasting rudimental herbicide of clomazone in high efficiency in field capacity 40% sterile soil to 200 μ gkg
-1The degraded situation of De clomazone;
But Fig. 3 be the degradation bacteria W2 bacterial strain of degrading lasting rudimental herbicide of clomazone in high efficiency in field capacity 60% natural soils to 500 μ gkg
-1The degraded situation of De clomazone.
(5) embodiment
For example the present invention is done in more detail below and describes:
1, soil sample: the soil that picks up from long-term application long residual herbicide in the Harbin City, Heilongjiang Province city Northeast Agricultural University experiment practice base;
2, the enrichment of thalline with separate
The pedotheque of long-term application long residual herbicide is mixed, take by weighing 10g and join and contain 200mgL
-1In the 50ml enrichment culture liquid of clomazone, place 30 ℃, 120rmin
-1Shaking table cultivate, add the 10ml fresh medium later on weekly, increase the concentration of clomazone at every turn, make that its concentration is respectively 200,400,800,1600,2000,3000,4000,5000mgL
-1, the enrichment culture time was respectively 2 months, and nutrient solution Zhong clomazone concentration reaches 5000mgL
-1After, adaptability is cultivated a week, with plate dilution method it is coated on and contains 200mgL
-1On the minimal medium of clomazone, picking list bacterium colony, the line purifying is preserved the single bacterium colony behind the purifying.
Composition and the proportioning such as the table 1 of described substratum (liquid):
The composition and the proportioning of table 1 substratum (liquid)
| The substratum title | Medium component |
| Enrichment culture liquid minimal medium | Extractum carnis: 3g, peptone: 5g, NaCl:5g, K 2HPO 4: 5g, distilled water: 1000ml, pH:7.0-7.2 KH 2PO 4：1.70g，K 2HPO 4：5.17g，(NH 4) 2SO 4: 2.6g, water: 1000ml., salts solution: 10ml, the PH:7.0-7.2 salts solution constitutes: MgSO 4：19.5g，MnSO 4：5g，FeSO 4：5g， CaCl 2: 0.3g, water: 1000ml |
Claims (3)
- But 1, a kind of degradation bacteria of degrading lasting rudimental herbicide of clomazone in high efficiency is characterized in that: it is classification called after Achromobacter xylosoxidans wood sugar oxidation subspecies (Achromobacter xylosoxidans ssxylosoxidans); Have following characteristic: optimum growth temperature is 30 ℃, and optimal pH is 6.0-9.0, can be the sole carbon source growth Yi clomazone, and viable count just can reach and surpass 100,000,000/gram or milliliter under common culture condition.
- But 2, a kind of cultural method of degradation bacteria of degrading lasting rudimental herbicide of clomazone in high efficiency is characterized in that:(1) soil sample: the soil that picks up from the long-term application long residual herbicide;(2) enrichment of thalline with separateThe pedotheque of long-term application long residual herbicide is mixed, take by weighing 10g and join and contain 200mgL -1In the 50ml enrichment culture liquid of clomazone, place 30 ℃, 120rmin -1Shaking table cultivate, add the 10ml fresh medium later on weekly, increase the concentration of clomazone at every turn, make that its concentration is respectively 200,400,800,1600,2000,3000,4000,5000mgL -1, the enrichment culture time was respectively 2 months, and nutrient solution Zhong clomazone concentration reaches 5000mgL -1After, adaptability is cultivated a week, with plate dilution method it is coated on and contains 200mgL -1On the minimal medium of clomazone, picking list bacterium colony, the line purifying is preserved the single bacterium colony behind the purifying.
- But 3, the cultural method of the degradation bacteria of degrading lasting rudimental herbicide of clomazone in high efficiency according to claim 2 is characterized in that: the composition of described substratum or liquid and proportioning are:
The substratum title Medium component Enrichment culture liquid minimal medium Extractum carnis: 3g, peptone: 5g, NaCl:5g, K 2HPO 4: 5g, distilled water: 1000ml, pH:7.0-7.2 KH 2PO 4：1.70g，K 2HPO 4：5.17g，(NH 4) 2SO 4: 2.6g, water: 1000ml., salts solution: 10ml, the PH:7.0-7.2 salts solution constitutes: MgSO 4：19.5g，MnSO 4：5g，FeSO 4：5g， CaCl 2: 0.3g, water: 1000ml
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200710071616 CN101003787A (en) | 2007-01-09 | 2007-01-09 | Degradation bacteria capable of degrading lasting rudimental herbicide of clomazone in high efficiency, and cultivation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200710071616 CN101003787A (en) | 2007-01-09 | 2007-01-09 | Degradation bacteria capable of degrading lasting rudimental herbicide of clomazone in high efficiency, and cultivation method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101003787A true CN101003787A (en) | 2007-07-25 |
Family
ID=38703167
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200710071616 Pending CN101003787A (en) | 2007-01-09 | 2007-01-09 | Degradation bacteria capable of degrading lasting rudimental herbicide of clomazone in high efficiency, and cultivation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101003787A (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101463337B (en) * | 2009-01-06 | 2011-01-05 | 东北农业大学 | A strain of Achromobacter xylosoxidans and use thereof for depredating clomazone |
| CN102417887A (en) * | 2011-09-08 | 2012-04-18 | 武汉科技大学 | Achromobacter xylosoxidans and application thereof to degradation of isoquinoline |
| CN103122332A (en) * | 2013-02-27 | 2013-05-29 | 中蓝连海设计研究院 | Achromobacter xylosoxidans subsp.xylosoxidans LH-N25 and heterotrophic nitrification and aerobic denitrification microorganism bactericide and preparation method and use thereof |
| CN103179862A (en) * | 2010-10-25 | 2013-06-26 | 马罗内生物创新公司 | Chromobacterium bioactive compositions and metabolites |
| CN104845902A (en) * | 2015-02-09 | 2015-08-19 | 暨南大学 | Application of Achromobacter sp. MT-H in degradation of di-2-ethyl hexyl phthalate (DEHP) |
| CN104845901A (en) * | 2015-02-09 | 2015-08-19 | 暨南大学 | Method for degrading di-2-ethyl hexyl phthalate (DEHP) by using Achromobacter sp. |
| CN109758713A (en) * | 2019-01-26 | 2019-05-17 | 吉林省农业科学院 | A kind of biological degradation method of sulfonylurea herbicide |
-
2007
- 2007-01-09 CN CN 200710071616 patent/CN101003787A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101463337B (en) * | 2009-01-06 | 2011-01-05 | 东北农业大学 | A strain of Achromobacter xylosoxidans and use thereof for depredating clomazone |
| CN103179862A (en) * | 2010-10-25 | 2013-06-26 | 马罗内生物创新公司 | Chromobacterium bioactive compositions and metabolites |
| CN103179862B (en) * | 2010-10-25 | 2015-09-16 | 马罗内生物创新公司 | Look bacillus biological activity and metabolite |
| CN102417887A (en) * | 2011-09-08 | 2012-04-18 | 武汉科技大学 | Achromobacter xylosoxidans and application thereof to degradation of isoquinoline |
| CN103122332A (en) * | 2013-02-27 | 2013-05-29 | 中蓝连海设计研究院 | Achromobacter xylosoxidans subsp.xylosoxidans LH-N25 and heterotrophic nitrification and aerobic denitrification microorganism bactericide and preparation method and use thereof |
| CN104845902A (en) * | 2015-02-09 | 2015-08-19 | 暨南大学 | Application of Achromobacter sp. MT-H in degradation of di-2-ethyl hexyl phthalate (DEHP) |
| CN104845901A (en) * | 2015-02-09 | 2015-08-19 | 暨南大学 | Method for degrading di-2-ethyl hexyl phthalate (DEHP) by using Achromobacter sp. |
| CN104845901B (en) * | 2015-02-09 | 2018-06-05 | 暨南大学 | A kind of method using achromobacter degradation diisooctyl phthalate |
| CN109758713A (en) * | 2019-01-26 | 2019-05-17 | 吉林省农业科学院 | A kind of biological degradation method of sulfonylurea herbicide |
| CN109758713B (en) * | 2019-01-26 | 2021-02-05 | 吉林省农业科学院 | Biodegradation method of sulfonylurea herbicide |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101003787A (en) | Degradation bacteria capable of degrading lasting rudimental herbicide of clomazone in high efficiency, and cultivation method | |
| Bedi et al. | Characterization of Rhizobium isolated from root nodules of Trifolium alexandrinum | |
| CN101463337B (en) | A strain of Achromobacter xylosoxidans and use thereof for depredating clomazone | |
| CN102827794B (en) | Pseudomonas mediterranea strain and application thereof | |
| CN101948782B (en) | Rhodopseudomonas spheroides strain and liquid inoculant thereof as well as preparation method and application thereof | |
| CN116144529B (en) | Rice saxophone OOR3-1 strain and application thereof | |
| CN104628429B (en) | A kind of preparation method of photosynthetic bacterial fertilizer | |
| CN116478870A (en) | Maltophilous oligotrophic single spore fungus OLR3-17 strain and application thereof | |
| CN101935627B (en) | Bromoxynil octanoate degrading bacteria and bacterial agent prepared from same | |
| Kuo et al. | Isoxazolin-5-ones and amino acids in root exudates of pea and sweet pea seedlings | |
| CN101463338B (en) | Bacillus subtilis and use thereof for degrading imazethapyr | |
| CN107628894A (en) | Composite bacteria agent increase soil fertility and its preparation method and application | |
| CN112522139B (en) | Achromobacter and application thereof in degradation of p-toluenesulfonic acid and quizalofop-p-ethyl | |
| CN100596305C (en) | Atrazine herbicide degradation bacterium preparation making method | |
| CN111533586B (en) | Chicken manure bio-organic fertilizer and preparation method thereof | |
| Kumar et al. | Isolation and characterization of drought tolerant bacteria from rice phyllosphere | |
| CN100371436C (en) | Bacterium for degrading chlorpyrifos pesticide residue and produced bacterium formulation | |
| CN101270346B (en) | Butachlor degradation bacterium and application thereof | |
| Cheruel et al. | Amino acid uptake into cultivated mesophyll cells from Asparagus officinalis L | |
| CN101693883B (en) | Microbial agent for degrading phoxim pesticide and preparation method thereof | |
| Emtsev et al. | Protective effect of Klebsiella bacteria on lawn grasses under conditions of soil salinization | |
| CN101457213B (en) | Marinococcus and its application in degrading imazethapyr | |
| CN108753629B (en) | Rhizopus oryzae with salt tolerance and application thereof | |
| RU2751487C1 (en) | Method for producing liquid nutrition medium and method for obtaining liguid microbiological agent based on strains mixture of spore-forming bacteria-antagonists of phytopathogenic fungi of g. fusarium | |
| CN111154668B (en) | Pseudomonas chlorous pyrolysis strain and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |