CN100595270C - Microorganism capable of hydrolyzing glycosyl to hydroxyl and application thereof - Google Patents
Microorganism capable of hydrolyzing glycosyl to hydroxyl and application thereof Download PDFInfo
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- CN100595270C CN100595270C CN200610046296A CN200610046296A CN100595270C CN 100595270 C CN100595270 C CN 100595270C CN 200610046296 A CN200610046296 A CN 200610046296A CN 200610046296 A CN200610046296 A CN 200610046296A CN 100595270 C CN100595270 C CN 100595270C
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- glycosyl
- wood sugar
- taxol
- deacetylate
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Abstract
The present invention relates to a method of hydrolyzing glycosyl to hydroxyl using enzyme, more specificly a microbe for hydrolyzing glycosyl to hydroxyl and its uses. The above-mentioned microbe isLeifsonia shinshuensis DICP 16,CCTCC No.M 206026.Strain DICP 16 or its fermented hydrolase contact taxane containing C-7 glycosyl and hydrolyzing C-7 glycosyl,the position being converted to hydroxy.One or several glycosyl especially C-7 xylosy being hydrolyzed to become a hydroxy. The resulting compound may be pharmaceutically active taxane such as paclitaxel or its analog, and also be intermediate with pharmaceutical activity. The invention relates to a method for hydrolyzing glycosyl to hydroxy, more specificly a microorganism for hydrolyzing glycosyl to hydroxy and its uses. The said microorganism is Leifsonia shinshuensis DICP 16, CCTCC No.M 206026.
Description
Technical field
The present invention relates to the method that the glycosyl enzymic hydrolysis is a hydroxyl, specifically a kind ofly change C-7 glycosyl Taxan into the microorganism of C-7 hydroxyl Taxan and the lytic enzyme of generation thereof, one or more glycosyls, especially the C-7 xylosyl of Taxan, be hydrolyzed under the microbial process of enzyme or product enzyme, glycosyl changes hydroxyl into.The gained compound can be Taxan such as taxol and the paclitaxel analogs with pharmacological activity, also can be the useful as intermediates that is used to prepare this type of tool pharmacologically active Taxan.
Background technology
Taxan is a class diterpene compound, is one of the most effective anticarcinogen in more than 10 year in the past, at pharmacy field significant application value is arranged.For example, taxol (Taxo1) is a kind of Taxan with following structure,
Wherein Ph is a phenyl, and Ac is an ethanoyl, and Bz is a benzoyl.
Taxane compounds such as taxol extensively are present in stem, leaf and the bark of Chinese yew genus plants, from the Japanese yew plant, separated at present obtain 300 surplus kind of Taxan diterpene-kind compound (J.Nat.Prod.1999,62,1448-1472).But the content of taxol is very low in the taxus extract, only is 0.0001～0.069%, average 0.015%; And coexistent impurity is of a great variety, and it is big directly to extract difficulty, and yield is low.Every extraction produce 1 kilogram of taxol need 3000 Cheng Shu of felling.And 1 kilogram of taxol only can satisfy 500 patients' medication.Therefore worldwide just launching unprecedented multidisciplinary, multi-level broad research around taxol resources problem.
Ramulus et folium taxi cuspidatae is considered to the renewable resources of at present tool utility value.Contain multiple bearing taxanes in its branches and leaves extract and have the mother nucleus structure identical with taxol, these compounds can be converted into semi-synthetic intermediate such as the baccatin III and the 10-deacetylate baccatin III (10-DAB III) of taxol by biology and chemical process.In numerous Chinese yew genus plants, extensively there is the Taxan of C-7 xylosyl, it is to the utilization of limited Taxan resources effective that this compounds is converted into the C-7 oxy-compound that can be utilized by the biological or chemical means.
Hanson RL etc. find Moraxella sp. (Mohs bacillus), Bacillus macerans (soaking numb genus bacillus), Bacillus circulans (Bacillus circulans) and Micrococcus sp. (micrococci) can produce the xylobiase (Biotechnol.Appl.Biochem.1997 that transforms 7-wood sugar Taxan, 26,153-158; European patent 95300135.1), wherein the conversion capability of Mohs bacillus is stronger, and 62.5mg Mohs bacillus can be converted into 10-deacetylate taxol with 0.5mg7-wood sugar-10-deacetylate taxol fully in 7 hours.Do not see other relevant report.
Summary of the invention
The object of the present invention is to provide a kind of can be the microorganism and the application thereof of hydroxyl with glycosyl hydrolase, can be with the Taxan of C-7 hydroxyl from the taxanes preparation of band C-7 glycosyl (especially xylosyl).The present invention uses the Taxan and the hydrolysis C-7 glycosyl of the enzyme at least a C-7 of the containing glycosyl of contact (especially xylosyl) of a kind of microorganism or this microorganisms, makes this position become hydroxyl.This method can prepare the Taxan of at least a C-7 of containing hydroxyl.
For achieving the above object, the technical solution used in the present invention is:
A kind of can be the microorganism of hydroxyl with glycosyl hydrolase, and described microorganism is Leifsohiashinshuensis DICP 16, CCTCC No.M 206026.
The lytic enzyme that the microorganism Leifsonia shinshuensis sp. bacterial strain DICP 16 that described biology is pure or its fermentation produce contacts at least a Taxan and the hydrolysis C-7 glycosyl that contains C-7 glycosyl (especially xylosyl), makes this position become hydroxyl.
Molecular structure with a kind of Taxan of method provided by the invention preparation is as follows:
R
1Be hydroxyl or acyloxy, especially work as R
1When having the structure of molecule I II;
R
2Be acyloxy or hydroxyl;
R
3And R
4Be independently hydrogen, alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkenyl, aromatic base or heterocyclic radical.
The molecular structure of a kind of substrate of enzyme contact is in the method provided by the invention:
R
1, R
2, R
3And R
4Implication with above-mentioned, " sugar " is meant the glycosyl of Direct Bonding in the C-7 position, the lytic enzyme that produces with bacterial strain DICP 16 or its fermentation can hydrolysis C-7 glycosyl, makes this position become hydroxyl.
Method for hydrolysis provided by the invention has been considered all steric configurations of the chiral centre of the compound with molecular structure I and II, and these steric isomers or be hydrolyzed separately perhaps mix and are hydrolyzed with other steric isomer.
The practical value of method provided by the invention is: Taxan is a class diterpene compound, at pharmacy field significant application value is arranged.The Taxan that extracts from plant is the mixture of various Taxans normally, and wherein one or more Taxans have the C-7 glycosyl, and only the Taxan (as taxol) of row band C-7 hydroxyl is only the product of finally wanting.The C-7 xylosyl of one or more C-7 glycosyl, especially Taxans is hydrolyzed under the lytic enzyme effect of bacterial strain DICP 16 or its fermentation generation, and glycosyl becomes the footpath base; The gained compound can be Taxan such as taxol and the paclitaxel analogs with pharmacological activity, also can be the useful as intermediates that is used to prepare this type of tool pharmacologically active Taxan.
The feasible steric configuration that is hydrolyzed the C-7 group of Taxan of method provided by the invention preferentially remaines in the product.The substituent absolute steric configuration of C-7 is identical with the absolute steric configuration of taxol C-7 hydroxyl.
Method provided by the invention is very little very effective when being used for from the taxanes preparation band C-7 hydroxyl Taxan of band C-7 glycosyl (especially xylosyl).Can a kind of taxane compounds of hydrolysis with method provided by the invention, also can melanges sequential property or the different taxane compounds of hydrolysis simultaneously.The Taxan mixture that is extracted by vegetable material is with method hydrolysis provided by the invention, and effect is fine.This mixture is used for the parent material of industrial preparation, wherein not only contains the Taxan of one or more band C-7 glycosyls (especially xylosyl), also contains the Taxan with other C-7 group such as hydroxyl.
Embodiment
1, parent material
The used parent material of method provided by the invention can be any Taxan that can carry out the band C-7 glycosyl of said hydrolyzed reaction.The Taxan of band C-7 xylosyl can be that nature forms; also chemical conversion gained; as 7-wood sugar taxol; 7-wood sugar-10-deacetylate taxol; 7-xylose baccatin III, 7-wood sugar-10-deacetylate baccatin III, 7-wood sugar Cephalomannine; 7-wood sugar-10-deacetylate Cephalomannine, the 7-wood sugar of 7-wood sugar taxol C or 7-wood sugar-10-deacetylate taxol C and chemical conversion gained-10-deacetylate baccatin III.The above-mentioned Taxan that forms naturally can come the cell cultures of self-produced Taxan plant or/and extract self-produced Taxan plant, especially Chinese yew genus plants such as southerm yew, taxusyunnanensis, the China Ramulus et folium taxi cuspidatae, european yew (berry Ramulus et folium taxi cuspidatae), ground hemlock, Xizang Taxus chinensis, Japan Ramulus et folium taxi cuspidatae (taxus chinensis in northeast), Pacific Ocean yewtree, Taxus x media and Himalaya Ramulus et folium taxi cuspidatae etc.The available plant tissue comprises tree root, needle, bark and sapling etc.
2. enzyme and microorganism
Enzyme that uses in the method for hydrolysis of the present invention or microorganism can be describe below any can katalaze enzyme the enzyme or the microorganism of hydrolysis reaction.These enzymes or microbial material no matter its source or purity all can be used under unbound state, perhaps are fixed on it on upholder with physical adsorption or method for entrapping and use.The microorganism Leifsonia shinshuensis that biology is pure is the microorganism of newfound product xylobiase.The mutant of this microorganism, for example through chemistry, (as the uv-radiation) of physics or biological method (as Protocols in Molecular Biology) transform to be used for the mutant strain of hydrolysis reaction, also in limit of consideration of the present invention.
Related Leifsonia shinshuensis DICP 16 among the present invention is deposited in Chinese typical culture collection center on March 20th, 2006, and preserving number is CCTCC No.M 206026.
The feature of Leifsonia shinshuensis: the gram-positive bacillus, do not form spore, aerobic growth on multiple substratum is grown to 0.3-0.4 * 2.5-3.0mm through cultivation in two days, the bacterium colony smooth surface, white colony is placed at 30 ℃ long-term and is transferred light brown to.Bacterium can grow in the time of 42 ℃, and is not long in the time of 45 ℃.Main menaquinone is MK-11 and MK-12 in the mycetocyte film.The G+C content of bacterium genomic dna is 71mol%.Peptone matrix-, bacitracin-, Ao Putuoqin-, hemicellulase+, urea+, TCC+, wood sugar-.The Ramulus et folium taxi cuspidatae pedotheque that separation obtains since Dalian Chemical Physics Research Institute.
The enzyme that the present invention uses is a lytic enzyme, especially glycanase or Glycosylase.These enzymes of production by biological provided by the invention, the method for available extraction and purifying is separated them.Microorganism provided by the invention can be with the form of complete wet cell, and perhaps with freeze-drying, the form of spraying drying or heat drying perhaps is applied to hydrolysis reaction with the form after handling through broken extracting.The form of these microorganisms after the genetic engineering improvement is also in limit of consideration of the present invention.Host cell can be any cell, as intestinal bacteria, changes a gene or one group of gene of microorganism provided by the invention over to host, makes it express a required enzyme or a plurality of enzyme of catalytic hydrolysis reaction.
Method for hydrolysis provided by the invention can carry out (two stage fermentations and hydrolysis) behind microbial fermentation, also can carry out (single phase original position fermentation and hydrolysis) with fermentation simultaneously.
Medium pH is between 4-7, and culture temperature is between 28-37 ℃.Hydrolysis reaction carried out 1-72 hour, reached maximum until the purpose product production.During hydrolysis reaction, pH remains on≤and 7.PH 4-7 is effective.
The present invention uses a kind of aqueous phase liquid as the hydrolysis reaction medium, and organic liquid or organic/water two-phase liquid also can be used.The present invention uses the Taxan of band C-7 position glycosyl of 0.0025-2.5% (w/v) as parent material.
Surpass 90% (comparing the per-cent of C-7 position hydrolysate) with method productive rate provided by the invention with the Taxan of initial band C-7 position glycosyl.Optionally reach the hydrolysis of C-7 position, promptly obtained product has only the hydrolysis of C-7 position, does not have the hydrolysis of other position.
3. separate
The product of band provided by the invention C-7 position hydroxyl can separated purifying, for example with extracting distillation, the method for crystallization and column chromatography.
4. use
Taxan is a class diterpene compound, and its structure is as above-mentioned.13 Taxan such as taxols that contain a side chain have pharmacologically active, are good anticarcinogens, can treat mammary cancer, ovarian cancer, colorectal carcinoma, lung cancer, melanoma and leukemia etc.
Compound that method for hydrolysis provided by the invention obtains such as taxol are extremely useful medicines, and can be used as intermediate and prepare 13 taxaneses that contain a side chain with pharmacologically active.With the compound of this law preparation such as hydroxyl of C-13 bit strip of baccatin III or 10-deacetylate baccatin III, can with midbody compound such as beta-lactam coupling, to obtain taxanes such as the taxol or the analogue of band C-13 position acyloxy side chain.Compound with the band C-7 position hydroxyl of this law preparation can modified coupling for use in above-mentioned C-13 position acyloxy side chain.For example, other the locational one or more hydroxyls beyond the C-13 position are protected before coupling, remove protection afterwards.
5. purpose compound
Method for hydrolysis of the present invention is fit to the Taxan that hydrolysis has molecular structure II very much, can provide the respective compound with molecular structure I through enzymic hydrolysis.In molecular structure I and II, R
2Be preferably hydroxyl or R
5-C (O)-O-especially works as R
5During for alkyl such as methyl; R
3Be preferably alkyl such as methyl; R
4Be preferably aromatic base such as phenyl; R
1Be preferably the group of hydroxyl or molecular structure III:
R wherein
6And R
7Be alkyl independently, alkenyl, alkynyl, cycloalkyl, cycloalkenyl, aromatic base, heterocycle or heterocyclic oxy group; R
8Be hydrogen, carbalkoxy or hydroxy-protective group.
Taxan with molecular structure II is: 7-wood sugar taxol; 7-wood sugar-10-deacetylate taxol; 7-wood pool baccatin III; 7-wood sugar-10-deacetylate baccatin III; 7-wood sugar Cephalomannine; 7-wood sugar-10-deacetylate Cephalomannine, 7-wood sugar taxol C and 7-wood sugar-10-deacetylate taxol C etc.Taxan with molecular structure I is: taxol, 10-deacetylate taxol, baccatin III, 10-deacetylate baccatin III, Cephalomannine, 10-deacetylate Cephalomannine, taxol C and 10-deacetylate taxol C etc.
The present invention is described further in conjunction with specific embodiments:
Embodiment 1: the screening of xylosidase
Prepare agar plate: 1% xylan, 0.05% yeast extract, 0.05% Tryptones, 1.5% agar.With 40 ml waters suspension, 0.2 gram pedotheque or rotten wooden sample, 1: 200 dilution suspension of each dull and stereotyped coating 50 microlitre, 50 microlitres, 0.25 mg/ml 7-wood sugar-10-deacetylate taxol.Hatch a week in room temperature (22 ℃), on the bacterium colony that grows, drip 1mM 4-methyl umbrella shape base-β-D-xyloside.71 under ultraviolet lamp fluorescigenic bacterium colony chosen, hatched 3-6 days in 28 ℃.
Shake-flask culture base composition: 1% xylan, 0.2% yeast extract, 0.2% Tryptones, 0.1%K
2HPO
4, 0.1%KH
2PO
4At 28 ℃, cultivate under the 175rpm condition.Add the 1 milligram of 7-wood sugar-10-deacetylate taxol or the 7-wood sugar-10-deacetylate baccatin III that are dissolved in 0.2 ml methanol two days later in 10 milliliters of nutrient solutions, continue to cultivate two days.
Another kind method is to cultivate one day earlier, cultivates three days after adding substrate again.
Centrifugation cell is resuspended in it in methyl alcohol, carries out TLC and HPLC and analyzes.In 71 bacterium colonies, 23 the product spot occurs in TLC.In these 23 positive bacterium colonies, the 10-deacetylate taxol of 4 generations (or 10-deacetylate baccatin III) amount is more, occurs tangible product peak in HPLC.The production concentration that is obtained by bacterium Leifsonia shinshuensis is the highest, and this bacterium is used for following examples after drawing the plate purifying once more.
The hydrolysis of embodiment 2:7-wood sugar-10-deacetylate taxol
Put into 1 liter of 1% birch xylan in 4 liters of Erlenmeyer culturing bottles, 0.2% yeast extract, 0.2% Tryptones, O.1%KH
2PO
4And 0.1%K
2HPO
4, pH 7.20 milliliters of bacterium liquid are inoculated in this substratum, 30 ℃ of shake-flask culture 3 days.Centrifugal collecting cell with 50 milliliters of 50mM potassium phosphate buffers (pH 7) washings, is resuspended in this damping fluid after centrifugal.In 1.5 ml cells suspensions, add 1mg 7-wood sugar-10-deacetylate taxol (being dissolved in 0.2 ml methanol and 0.3 ml water).100rpm, 30 ℃ of mixings 21 hours.Add 2 ml methanol termination reactions, carry out HPLC and analyze.No 7-wood sugar-10-deacetylate taxol is residual in the reaction solution, and produces 0.4 mg/ml 10-deacetylate taxol (productive rate 100%).This routine reaction process is as follows:
The hydrolysis of embodiment 3:7-wood sugar-taxol
In 1.5 ml cells suspensions (preparation method is with example 3), add 0.5mg 7-wood sugar-taxol (being dissolved in 0.2 ml methanol and 0.3 ml water).100rpm, 30 ℃ of mixings 21 hours.Add 2 ml methanol termination reactions, carry out HPLC and analyze.Residual 0.01 mg/ml 7-wood sugar-taxol in the reaction solution, and produce 0.17 mg/ml taxol (productive rate 77%).
The HPLC method
Post: Kromasil ODS (4.6 * 200mm, 5 μ m)
Moving phase: 60% methyl alcohol/40% water
Flow velocity: 1 ml/min
Column temperature: room temperature
Detect wavelength: 227nm
The hydrolysis of embodiment 4:7-wood sugar-baccatin III
Bacterium is in 30 ℃ of shake-flask culture, substratum: 2% glycerine, 0.2% yeast extract, 0.2% Tryptones, 0.1%KH
2PO
4, 0.1%K
2HPO
41 milliliter of bacterium liquid is inoculated in 100 milliliters of substratum.Taking out 15 milliliters after 4 days is inoculated in 1 liter of substratum (4 liters of Erlenmeyer culturing bottles).3 big back centrifugal collecting cells are with 50mM potassium phosphate buffer (pH 7) washing, freezing preservation behind the recentrifuge.
2 milliliters of solution that contain 0.2mg 7-wood sugar-baccatin III add 1 milliliter of above-mentioned bacterium liquid (0.24 gram wet cell weight), 100rpm mixing 24 hours.Reaction solution is with 6 milliliters of CH
2Cl
2Extracting.Be dissolved in again after the extract drying and carry out the HPLC analysis in the methyl alcohol.Detect baccatin III (productive rate 102% calculates with initial 7-wood sugar-taxol concentration).
The hydrolysis of embodiment 5:7-wood sugar-10-deacetylate baccatin III
The cell preparation method is with embodiment 4.1 milliliter of solution that contains 1mg 7-wood sugar-10-deacetylate baccatin III adds 1 milliliter of bacterium liquid (0.24 gram wet cell weight), 100rpm mixing 24 hours.Reaction solution is with 6 milliliters of CH
2Cl
2Extracting.Be dissolved in again after the extract drying and carry out the HPLC analysis in the methyl alcohol.Detect 10-deacetylate baccatin III (productive rate 100% calculates with initial 7-wood sugar-10-deacetylate baccatin III concentration).
The hydrolysis of embodiment 6:7-wood sugar-10-deacetylate Cephalomannine
The cell preparation method is with embodiment 4.2 milliliters of solution that contain 0.2mg 7-wood sugar-10-deacetylate Cephalomannine add 1 milliliter of bacterium liquid (0.24 gram wet cell weight), 100rpm mixing 24 hours.Reaction solution is with 6 milliliters of CH
2Cl
2Extracting.Be dissolved in again after the extract drying and carry out the HPLC analysis in the methyl alcohol.Detect 10-deacetylate Cephalomannine (productive rate 97% calculates with initial 7-wood sugar-10-deacetylate Cephalomannine concentration).
Claims (2)
1. taxanes xyloside desaccharification microorganism, it is characterized in that: described microorganism is Leifsoniashinshuensis DICP 16, CCTCC No.M 206026.
2. described application of microorganism of claim 1 is characterized in that: the lytic enzyme that described microorganism Leifsonia shinshuensis.DICP 16 or its fermentation produce, and contact contains the Taxan and the hydrolysis C-7 glycosyl of C-7 glycosyl, makes this position become hydroxyl; The Taxan that described contact contains the C-7 glycosyl is a 7-wood sugar taxol; 7-wood sugar-10-deacetylate taxol; 7-xylose baccatin III; 7-wood sugar-10-deacetylate baccatin III; 7-wood sugar Cephalomannine; 7-wood sugar-10-deacetylate Cephalomannine, the 7-wood sugar of 7-wood sugar taxol C or 7-wood sugar-10-deacetylate taxol C and chemical conversion gained-10-deacetylate baccatin III.
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| CN102296053B (en) | 2010-06-25 | 2015-03-11 | 中国医学科学院药物研究所 | 7-xylosyltaxonoglycosyl hydrolase, nucleotide sequence of gene thereof, and application thereof |
| CN102363612B (en) * | 2011-11-03 | 2013-06-19 | 赵华 | Method for extracting taxol simply, conveniently and efficiently |
| CN103864729A (en) * | 2014-03-03 | 2014-06-18 | 无锡紫熙生物科技有限公司 | Preparation method of 10-deacetylbaccatin III |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1209059A (en) * | 1995-12-21 | 1999-02-24 | 基因实验室技术有限公司 | Taxane compositions and method for producing same |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1209059A (en) * | 1995-12-21 | 1999-02-24 | 基因实验室技术有限公司 | Taxane compositions and method for producing same |
Non-Patent Citations (2)
| Title |
|---|
| 紫杉烷类化合物的研究进展. 王春霖，曹聪梅，史清文等.河北医药，第27卷第9期. 2005 * |
| 红豆杉中紫杉烷类化合物制备10-去乙酰基巴卡亭Ⅲ. 杨陶，李明，陈建民.复旦学报(自然科学版)，第43卷第6期. 2004 * |
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