CN100570353C - Two-channel self calibrating multiple parameters rapid whole blood biochemistry analyzing sensor - Google Patents
Two-channel self calibrating multiple parameters rapid whole blood biochemistry analyzing sensor Download PDFInfo
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- CN100570353C CN100570353C CNB2007100697805A CN200710069780A CN100570353C CN 100570353 C CN100570353 C CN 100570353C CN B2007100697805 A CNB2007100697805 A CN B2007100697805A CN 200710069780 A CN200710069780 A CN 200710069780A CN 100570353 C CN100570353 C CN 100570353C
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Abstract
The invention discloses a kind of two-channel self calibrating multiple parameters rapid whole blood biochemistry analyzing sensor, comprise the upper strata hydrophilic coating film, lower floor's sensor substrate and demarcation liquid bag, lower floor's sensor substrate is provided with demarcates liquid passage and test fluid passage, each passage is by the injection region, miniflow raceway groove and waste liquid recovery pond constitute, symmetric offset spread has some to blood chemistry in the two miniflow raceway grooves, blood electrolyte and vim and vigour potential electrode, constitute respectively and demarcate electrod-array and array of measure electrodes, the biological chemical reagent of reaction takes place to measure in the participation that is solidified with identical type on two working electrodes of every pair of electrode, fixing different types of enzyme or electrolyte and the vim and vigour sensitive materials of between every pair of electrode.The present invention utilize same batch make and closely adjacent electrode have height consistance and simple channel structure and solved on-site proving problem during rapid whole blood is analyzed, need not additional electromechanical structure, improve measuring speed greatly, reduce testing cost.
Description
Technical field
The present invention relates to a kind of biology sensor, especially relate to a kind of two-channel self calibrating multiple parameters rapid whole blood biochemistry analyzing sensor.
Background technology
The clinical biochemical check is the indispensable means of blood component analyzing.The check of tradition clinical biochemical is carried out on the large-scale Biochemical Analyzer of hospital, need to detect the pollution-free transmission of sample, carry out via professional operating personnel, consuming time and expensive, the trend that can't adapt to domestic medicine also can't satisfy the demand of " time is life " occasions such as operation, emergency treatment, open-air rescue.POCT (Point of CareTesting-POCT) can allow the doctor make the clinical judgment of full blast in the shortest time, brings revolutionary progress to medical treatment.At present, the POCT project mainly concentrates in blood biochemical, blood electrolyte, vim and vigour and the blood clotting velocity analysis.POCT can carry out in emergency ward, operating room, outpatient service and the patient bedside of hospital; Occasions such as clinic that also can be outside hospital, clinic, ambulance, the scene of the accident, the scene of donating blood, family, recruitment point are carried out.Can shorten round of visits, patient is in time made diagnosis and treats, prevents that acute sb.'s illness took a turn for the worse, help medical first aid, also can test oneself at home, disease is monitored by patient.
Be applied among the POCT, biochemistry detection technological means commonly used has two kinds of optics and galvanochemistry.Because electrochemical techniques, are more suitable for bedside and detect this purpose than the end, advantage such as simple to operate from required equipment simple relatively (need not complicated optical system), cost.On the technology trends, electrochemical method just is used widely.For example, aspect the test of blood sugar bedside, because the development of electrochemical measuring technique, traditional optical detecting method is substituted by electrochemical measuring technique basically at present.Have at the main POCT product of American market at present: i-STAT hand-held vim and vigour/pH+ blomelicalbloodgasandelectrolrteanalyzers that i-STAT company produces, the portable vim and vigour blomelicalbloodgasandelectrolrteanalyzers of IRMA 2000 types etc.Though the electrochemical sensing technology has been passed through years of researches and development, it still has a certain distance with clinical requirement on stability.The most practicable means that address this problem are " on-site provings ".But in document and the patent report fluid of demarcating liquid and tested blood is controlled many more complicated at present, need control elements such as pump, valve, for example the i-STAT hand-held vim and vigour/pH+ blomelicalbloodgasandelectrolrteanalyzers of i-STAT company production.
Summary of the invention
The invention provides a kind of with low cost, need not additional electromechanical structure in demarcating at the scene, at the two-channel self calibrating multiple parameters rapid whole blood analysis sensor array of basis and important detection content the most in the check of clinical biochemicals such as blood biochemical/electrolyte and vim and vigour.
A kind of two-channel self calibrating multiple parameters rapid whole blood biochemistry analyzing sensor, comprise the upper strata hydrophilic coating film, lower floor's sensor substrate, be located at the demarcation liquid bag between upper strata hydrophilic coating film and the lower floor's sensor substrate, described lower floor sensor substrate is provided with demarcates liquid passage and test fluid passage, each passage is by the injection region, miniflow raceway groove and waste liquid recovery pond constitute, symmetric offset spread has some to blood chemistry in the two miniflow raceway grooves, blood electrolyte and vim and vigour potential electrode, constitute respectively and demarcate electrod-array and array of measure electrodes, demarcate electrod-array and array of measure electrodes and form sensor array, the biological chemical reagent of reaction takes place to measure in the participation that is solidified with identical type on two working electrodes of every pair of electrode, fixing different types of enzyme or electrolyte and the vim and vigour sensitive materials of between every pair of electrode.
Described demarcation liquid passage and test fluid passage are respectively applied for the demarcation and the measurement of sensor array.
Described miniflow raceway groove inwall is all through hydrophilic treatment.
Described upper strata hydrophilic coating film and lower floor sensor substrate cooperation place are provided with the inlet of corresponding liquid passage to be measured.
Array of measure electrodes comprises several glucose and other enzyme electrodes, sensitive electrodes such as potassium, calcium, sodium, the quick electrode of hydrogen plasma and blood oxygen, carbon dioxide.
Demarcate electrod-array and comprise some glucose corresponding and other enzyme electrodes, sensitive electrodes such as potassium, calcium, sodium, the quick electrode of hydrogen plasma and blood oxygen, carbon dioxide with array of measure electrodes.
The present invention is solidified several different enzyme reagent, electrolyte and dissolved gas sensitive materials on the different electrode of same substrate, combine vim and vigour, electrolyte and biochemical substances quick test, be a typical POCT equipment, do not need new blood carried out pre-service such as centrifuging, just can be simultaneously to pH in the blood, pCO
2, PO
2, Na
+, K
+, Ca
++, the fast detecting that multiple parameters such as glucose carry out, duty, blood acid-base balance and ion concentration, the metabolism situation of reflection patient breathing system, content.The present invention utilizes siphon principle to suck liquid to be measured by inlet, discharge the demarcation liquid of demarcating in the liquid bag simultaneously, utilize in the same production batch, the height consistance of adjacent electrode and simple channel structure solved the on-site proving problem during rapid whole blood is analyzed, the electromechanical structure that need not to add (as motor, pump etc.).
Description of drawings
Fig. 1 is an one-piece construction synoptic diagram of the present invention.
Wherein, the sensor substrate 2-of 1-lower floor upper strata hydrophilic coating film 3-conductive electrode joint 4-demarcates liquid bag and storage pool 5-demarcation liquid miniflow raceway groove 6-array of measure electrodes 7-demarcation electrod-array 8-test fluid suction inlet 9-test whole blood sample miniflow raceway groove 10-electrode cable 11-test fluid recovery pond 12-demarcation liquid recovery pond
Embodiment
As shown in Figure 1, a kind of two-channel self calibrating multiple parameters rapid whole blood biochemistry analyzing sensor, comprise upper strata hydrophilic coating film 2, lower floor's sensor substrate 1, be located at the demarcation liquid bag 4 between upper strata hydrophilic coating film 2 and the lower floor's sensor substrate 1, lower floor's sensor substrate 1 is provided with demarcates liquid passage and test fluid passage, demarcates the liquid passage and is made of demarcation liquid injection region 4, miniflow raceway groove 5, demarcation liquid waste liquid recovery pond 12.The test fluid passage is made of liquid to be measured injection region 8, miniflow raceway groove 9, liquid waste liquid recovery pond 11 to be measured.
Symmetric offset spread has some to blood chemistry, blood electrolyte and vim and vigour potential electrode in the two miniflow raceway grooves 5,9, constitute respectively and demarcate electrod-array 7 and array of measure electrodes 6, the biological chemical reagent of reaction takes place to measure in the participation that is solidified with identical type on two working electrodes of every pair of electrode, fixing different types of enzyme or electrolyte and the vim and vigour sensitive materials of between every pair of electrode.
Array of measure electrodes 6 comprises several glucose and other enzyme electrodes, sensitive electrodes such as potassium, calcium, sodium, the quick electrode of hydrogen plasma and blood oxygen, carbon dioxide.
Demarcate electrod-array 7 and comprise some glucose corresponding and other enzyme electrodes, sensitive electrodes such as potassium, calcium, sodium, the quick electrode of hydrogen plasma and blood oxygen, carbon dioxide with array of measure electrodes.
During test, when sensor inserts corresponding measurement instrument, demarcating liquid bag 4 is fixed on after the extruding of mechanical protrusions on the instrument broken, the demarcation liquid that the discharges miniflow raceway groove 5 of flowing through, on demarcation electrod-array 7, react, the electric signal that is produced with known each constituent concentration comparison of demarcation liquid, correction measurement instrument parameter, reaches the effect of calibration sensor by the measurement instrument record.
When liquid to be measured injects test fluid suction inlet 8, at the dirty electrod-array after tested 6 of the capillary action of miniflow raceway groove 9, on test electrode array 6, react, carry out the test of each parameter.
Owing to demarcating 6 same batches of making and closely adjacent of electrod-array 7 and test electrode array, therefore with test electrode on a pair of and demarcation electrode performance basically identical.Flow through respectively at short notice when the demarcation liquid of liquid to be measured and known each concentration parameter and to demarcate electrod-array 7 and test electrode array 6, finish the on-site proving data acquisition and the in-site measurement data acquisition of sensor; The on-site proving data are used to calibrate the in-site measurement data, to eliminate measurement environment and storage time influence, the raising measurement accuracy to measuring accuracy; At last, liquid to be measured and demarcation liquid flow to liquid waste liquid recovery pond 11 to be measured respectively and demarcate liquid waste liquid recovery pond 12.
The present invention utilize same batch make and closely the consistance of adjacent electrode and binary channels micro-fluidic structure solved on-site proving problem during rapid whole blood is analyzed, do not need the electromechanical structure that adds.Adopt this design design and the manufacturing cost of facilitating chip greatly.
The last subtegulum of rapid whole blood analyzing and testing chip can be by polycarbonate, and high molecular polymerization materials such as poly-ethyl ester are made, and these materials are non-conductive, and are pliable and tough, and certain rigidity and intensity are arranged.Enzyme reagent and electron transfer mediator are solidificated in electrode surface formation biological elements, and different chemical reactions can take place with these enzyme electrodes respectively in the blood in the groove, thereby can measure the concentration of the compositions such as various ions, vim and vigour and glucose in the blood.
Claims (3)
1. two-channel self calibrating multiple parameters rapid whole blood biochemistry analyzing sensor, comprise the upper strata hydrophilic coating film, lower floor's sensor substrate, be located at the demarcation liquid bag between upper strata hydrophilic coating film and the lower floor's sensor substrate, it is characterized in that: described lower floor sensor substrate is provided with demarcates liquid passage and test fluid passage, each passage is by the injection region, miniflow raceway groove and waste liquid recovery pond constitute, symmetric offset spread has some to blood chemistry in the two miniflow raceway grooves, blood electrolyte and vim and vigour potential electrode, constitute respectively and demarcate electrod-array and array of measure electrodes, demarcate electrod-array and array of measure electrodes and form sensor array, the biological chemical reagent of reaction takes place to measure in the participation that is solidified with identical type on two working electrodes of every pair of electrode, fixing different types of enzyme or electrolyte and the vim and vigour sensitive materials of between every pair of electrode;
Wherein, described upper strata hydrophilic coating film and lower floor sensor substrate cooperation place are provided with the inlet of corresponding liquid passage to be measured.
2. sensor as claimed in claim 1 is characterized in that: described demarcation liquid passage and test fluid passage are respectively applied for the demarcation and the measurement of sensor array.
3. sensor as claimed in claim 1 is characterized in that: described miniflow raceway groove inwall is all through hydrophilic treatment.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2007100697805A CN100570353C (en) | 2007-06-29 | 2007-06-29 | Two-channel self calibrating multiple parameters rapid whole blood biochemistry analyzing sensor |
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|---|---|---|---|
| CNB2007100697805A CN100570353C (en) | 2007-06-29 | 2007-06-29 | Two-channel self calibrating multiple parameters rapid whole blood biochemistry analyzing sensor |
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| CN101082600A CN101082600A (en) | 2007-12-05 |
| CN100570353C true CN100570353C (en) | 2009-12-16 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108732211A (en) * | 2017-04-18 | 2018-11-02 | 中山大学 | SOLUTION PROPERTIES sensor and its manufacturing method |
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| CN103822944A (en) * | 2014-02-12 | 2014-05-28 | 绿谷（集团）有限公司 | Real-time on-line detection method and device of rapid biological signals |
| CN104820003B (en) * | 2015-05-13 | 2017-06-16 | 江苏大学 | A kind of papery microfluidic system and method for Detecting Pesticide |
| CN105241930A (en) * | 2015-09-24 | 2016-01-13 | 浙江大学 | Multiparameter all-solid-state blood analysis sensor |
| CN105353018A (en) * | 2015-10-23 | 2016-02-24 | 吴国清 | Multi-term ion biochemical analysis dry plate and preparation method thereof |
| DE102017208461A1 (en) | 2017-05-18 | 2018-11-22 | Diabetes.Online Ag | Multianalytmessung |
| CN108508074A (en) * | 2018-04-29 | 2018-09-07 | 浙江大学山东工业技术研究院 | A kind of anti-interference dual-port screen printing electrode |
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| CN217156402U (en) * | 2022-04-14 | 2022-08-09 | 深圳可孚生物科技有限公司 | Electrochemical sensor based on associated sensor |
| CN115406946B (en) * | 2022-08-16 | 2023-06-30 | 山东卓越生物技术股份有限公司 | PO (potential of Hydrogen) of disposable instant detection card and sensor chip 2 Electrode preparation method |
| CN116539701B (en) * | 2023-07-07 | 2023-09-15 | 北京几何科技有限公司 | Reagent card for urine detection and detection method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108732211A (en) * | 2017-04-18 | 2018-11-02 | 中山大学 | SOLUTION PROPERTIES sensor and its manufacturing method |
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