CN100567458C - A kind of method of utilizing lipase-catalyzed biodiesel synthesis - Google Patents
A kind of method of utilizing lipase-catalyzed biodiesel synthesis Download PDFInfo
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- CN100567458C CN100567458C CNB2006100179233A CN200610017923A CN100567458C CN 100567458 C CN100567458 C CN 100567458C CN B2006100179233 A CNB2006100179233 A CN B2006100179233A CN 200610017923 A CN200610017923 A CN 200610017923A CN 100567458 C CN100567458 C CN 100567458C
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- Prior art keywords
- lipase
- grease
- methyl alcohol
- reacts
- biodiesel synthesis
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P30/00—Technologies relating to oil refining and petrochemical industry
- Y02P30/20—Technologies relating to oil refining and petrochemical industry using bio-feedstock
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- Liquid Carbonaceous Fuels (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
The present invention relates to a kind of method of utilizing lipase-catalyzed biodiesel synthesis, add methyl alcohol in grease, the mol ratio of grease and methyl alcohol is 1: 1-2: 1; Reacting by heating to temperature is 45-55 ℃, adds lipase, and the lipase consumption is the 2.5%-8% of grease weight, reacts 6-12h under the agitation condition of 50-200rpm; Adding is 0.5 with the grease mol ratio then: 1-0.75: 1 methyl alcohol reacts 6-10h again, adds the remaining methyl alcohol of reaction at last and reacts 6-10h again; Then reaction solution is extracted out and left standstill 3-6h, isolate the raw glycerine of lower floor, the clear liquid on upper strata is extracted, extract pure biofuel.The present invention is not with an organic solvent; Alcohol oil remains at 1: 1-2: between 1, be best alcohol oil rate; Reaction conditions gentleness, mixing speed are not high, and required conversion unit is simple, and discharging is convenient, and the back is extracted easy; The lipase consumption is less, can reuse, and cleans the organic solvent of lipase and can reuse environmentally safe; Reaction times is short, the transformation efficiency height.
Description
Technical field
The present invention relates to a kind of method of biodiesel synthesis, relate in particular to a kind of method of utilizing lipase-catalyzed biodiesel synthesis.
Background technology
At present, the production of biofuel adopts chemical catalyst catalysis grease synthetic mostly, and the required temperature of this method is higher, the alcohol amount is excessive greatly, aftertreatment is difficult, environment is caused great harm.And about the method for lipase-catalyzed biodiesel synthesis, substantially all be under the situation that organic solvent exists, to carry out, and the use of organic solvent has increased cost on the one hand, has increased isolating difficulty on the other hand.And study and generally on breadboard shaking table, finish, seldom mention for bigger experimental installation.Because methyl alcohol has toxicity to lipase, the investigator who has adopts other acyl acceptor as reactant, adopts this kind method then to increase the complicacy of reaction; And the alcohol oil rate that Many researchers adopts is 1: 1, adds the alcohol reaction three times that carry out equivalent, and the reaction times is very long.
Summary of the invention
The object of the present invention is to provide a kind of method of utilizing lipase-catalyzed biodiesel synthesis,, reduce separating difficulty to reduce production costs.
To achieve these goals, technical program of the present invention lies in adopting a kind of method of utilizing lipase-catalyzed biodiesel synthesis, add methyl alcohol in grease, the mol ratio of grease and methyl alcohol is 1: 1-2: 1; Reacting by heating to temperature is 45-55 ℃, adds lipase, and the lipase consumption is the 2.5%-8% of grease weight, reacts 6-12h under the agitation condition of 50-200rpm; Adding is 0.5 with the grease mol ratio then: 1-0.75: 1 methyl alcohol reacts 6-10h again, adds the remaining methyl alcohol of reaction at last and reacts 6-10h again; Then reaction solution is extracted out and left standstill 3-6h, isolate the raw glycerine of lower floor, the clear liquid on upper strata is extracted, extract pure biofuel.
Described lipase is Novo435.
Described grease is a Vegetable oil lipoprotein.
Described Vegetable oil lipoprotein can be rapeseed oil, Oleum Verniciae fordii.
Present method adopts whipping appts, more than the 500ml, adopts periodical operation in batches, and raw materials used oil once all adds, and the present invention is not with an organic solvent; Alcohol oil remains at 1: 1-2: between 1, be best alcohol oil rate; Reaction conditions gentleness, mixing speed are not high, and required conversion unit is simple, and discharging is convenient, and the back is extracted easy; The lipase consumption is less, can reuse, and cleans the organic solvent of lipase and can reuse environmentally safe; Reaction times is short, the transformation efficiency height; Be very easy to carry out industry and amplify, the experiment of doing is finished in the above stirred reactor of 500ml.Lipase in present method can use 10-20 to criticize continuously, utilizes organic solvent to clean then, and the lipase after the cleaning then uses, and catalytic effect is constant.
Embodiment
Embodiment 1
The method of utilizing lipase-catalyzed biodiesel synthesis of present embodiment adds methyl alcohol in rapeseed oil, the mol ratio of grease and methyl alcohol is 1.5: 1; Reacting by heating to temperature is 50 ℃, adds Novo435 lipase, and Novo435 lipase consumption is 5% of a grease weight, reacts 6-12h under the agitation condition of 100rpm; Add then with the grease mol ratio be that 0.75: 1 methyl alcohol reacts 6h again, add the remaining methyl alcohol of reaction at last and react 6h again; Then reaction solution is extracted out and left standstill 3h, isolate the raw glycerine of lower floor, the clear liquid on upper strata is extracted, extract pure biofuel.Wherein, ester exchange rate reaches 95%.
Embodiment 2
The method of utilizing lipase-catalyzed biodiesel synthesis of present embodiment adds methyl alcohol in Oleum Verniciae fordii, the mol ratio of grease and methyl alcohol is 2: 1; Reacting by heating to temperature is 45 ℃, adds Novo435 lipase, and Novo435 lipase consumption is 8% of a grease weight, reacts 12h under the agitation condition of 100rpm; Add remaining methyl alcohol then, react 10h again; Then reaction solution is extracted out and left standstill 6h, isolate the raw glycerine of lower floor, the clear liquid on upper strata is extracted, extract pure biofuel.Wherein, ester exchange rate reaches 90%.
It should be noted last that: above embodiment is the unrestricted technical scheme of the present invention in order to explanation only, although the present invention is had been described in detail with reference to the foregoing description, those of ordinary skill in the art is to be understood that: still can make amendment or be equal to replacement the present invention, and not breaking away from any modification or partial replacement of the spirit and scope of the present invention, it all should be encompassed in the middle of the claim scope of the present invention.
Claims (4)
1, a kind of method of utilizing lipase-catalyzed biodiesel synthesis is characterized in that: add methyl alcohol in grease, the mol ratio of grease and methyl alcohol is 1: 1-2: 1; Reacting by heating to temperature is 45-55 ℃, adds lipase, and the lipase consumption is the 2.5%-8% of grease weight, reacts 6-12h under the agitation condition of 50-200rpm; Adding is 0.5 with the grease mol ratio then: 1-0.75: 1 methyl alcohol reacts 6-10h again, adds the remaining methyl alcohol of reaction at last and reacts 6-10h again; Then reaction solution is extracted out and left standstill 3-6h, isolate the raw glycerine of lower floor, the clear liquid on upper strata is extracted, extract pure biofuel.
2, the method for utilizing lipase-catalyzed biodiesel synthesis according to claim 1 is characterized in that: described lipase is Novo435.
3, the method for utilizing lipase-catalyzed biodiesel synthesis according to claim 1 is characterized in that: described grease is a Vegetable oil lipoprotein.
4, the method for utilizing lipase-catalyzed biodiesel synthesis according to claim 3 is characterized in that: described Vegetable oil lipoprotein is rapeseed oil or Oleum Verniciae fordii.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100179233A CN100567458C (en) | 2006-06-08 | 2006-06-08 | A kind of method of utilizing lipase-catalyzed biodiesel synthesis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100179233A CN100567458C (en) | 2006-06-08 | 2006-06-08 | A kind of method of utilizing lipase-catalyzed biodiesel synthesis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101085927A CN101085927A (en) | 2007-12-12 |
| CN100567458C true CN100567458C (en) | 2009-12-09 |
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| Application Number | Title | Priority Date | Filing Date |
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| CNB2006100179233A Expired - Fee Related CN100567458C (en) | 2006-06-08 | 2006-06-08 | A kind of method of utilizing lipase-catalyzed biodiesel synthesis |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010079500A2 (en) * | 2008-02-28 | 2010-07-15 | Reliance Life Sciences Pvt. Ltd | Aerobic production of 1,3-propanediol from crude glycerol from biodiesel process. |
| CN101418226B (en) * | 2008-12-01 | 2012-01-25 | 南京工业大学 | Method for preparing biodiesel by using N-heterocyclic carbine carbondioxide adducts as catalyst |
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2006
- 2006-06-08 CN CNB2006100179233A patent/CN100567458C/en not_active Expired - Fee Related
Non-Patent Citations (4)
| Title |
|---|
| 生物柴油的研究与进展. 徐桂转等.华中农业大学学报，第24卷第6期. 2005 |
| 生物柴油的研究与进展. 徐桂转等.华中农业大学学报，第24卷第6期. 2005 * |
| 脂肪酶催化合成生物柴油的研究. 邓利等.生物工程学报，第19卷第1期. 2003 |
| 脂肪酶催化合成生物柴油的研究. 邓利等.生物工程学报，第19卷第1期. 2003 * |
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| Publication number | Publication date |
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| CN101085927A (en) | 2007-12-12 |
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Granted publication date: 20091209 Termination date: 20100608 |