CN100531713C - Ginseng and aconite high concentration injection liquid for treating qi vacuity and yang desertion and its preparation method - Google Patents

Abstract

A Chinese medicine in the form of high-concentration injection for treating the deficiency of qi and yang is prepared from ginseng and prepared aconite root through extracting their liquid extracts, purifying, vacuum concentrating by 1-5 times, proportionally mixing, regulating pH to 5-7, loading in bottle and sterilizing. Its advantages are controllable quality and sure curative effect.

Description

A kind of attached high concentration injection of ginseng that deficiency of vital energy sun takes off and preparation method thereof for the treatment of

Invention field

The present invention relates to a kind of pharmaceutical preparation and preparation method thereof, particularly relate to and treat pharmaceutical preparation that deficiency of vital energy sun takes off and preparation method thereof.

Background of invention

Prior art discloses 'Shenfu ', and said preparation is that raw material feeds intake with the decoction pieces.

Summary of the invention

The object of the invention is to provide a kind of 'Shenfu ' that deficiency of vital energy sun takes off for the treatment of; The object of the invention also is to provide a kind of preparation method for the treatment of the 'Shenfu ' that deficiency of vital energy sun takes off.

The present invention seeks to be achieved through the following technical solutions:

One, preparation of the present invention is made by following raw material:

Radix Ginseng extract 1.5-18 weight portion, Radix Aconiti Lateralis Preparata extract 1.5-18 weight portion.

The above-mentioned raw materials optimum ratio is: Radix Ginseng extract 4.5-7.5 weight portion, Radix Aconiti Lateralis Preparata extract 9-15 weight portion.The above-mentioned raw materials optimum ratio is: Radix Ginseng extract 4-12 weight portion, Radix Aconiti Lateralis Preparata extract 4-12 weight portion.

Radix Ginseng extract of the present invention can be but be not limited to the alcohol extraction supersound extraction and cross the refining thing of post, water and carry that supersound extraction is crossed the refining thing of post, the refining thing of post is crossed in alcohol extraction or water was carried the refining thing of post.

Radix Aconiti Lateralis Preparata extract of the present invention can be but be not limited to the alcohol extraction supersound extraction and cross the refining thing of post, water and carry that supersound extraction is crossed the refining thing of post, the refining thing of post is crossed in alcohol extraction or water was carried the refining thing of post.

Above-mentioned refining thing can adopt membrane separation technique further refining, and the model of used film can be: FLT-Z (II)---FLT-VL; FLT-Z (II)---FLT-VG---FLT-VAB---FLT-VAC; FLT-VL.

Preparation of the present invention can add adjuvant and make clinical acceptable forms such as tablet, capsule, oral liquid, drop pill, spray, granule, injection, powder pin, freeze-dried powder, infusion solutions, high concentration injection, injection tablet, slow-releasing microcapsule, rapid release drop pill; Described adjuvant comprises solvent, disintegrating agent, correctives, antiseptic, coloring agent, binding agent, lubricant, substrate etc.

Two, the preparation method of Radix Ginseng extract, Radix Aconiti Lateralis Preparata extract can be but is not limited in the following method any one in the preparation of the present invention:

1, get the people and participate in 60～80% ethanol, supersound extraction 3～4 times, ultrasonic power is 250～800W, and each 20～45 minutes, the solvent consumption was 4～6 times of medical material amount, and merge extractive liquid, reclaims ethanol to there not being the ethanol flavor, gets the Radix Ginseng extractive solution; Get Radix Aconiti Lateralis Preparata and add 60～80% ethanol, supersound extraction 3～4 times, ultrasonic power is 250～800W, and each 20～45 minutes, the solvent consumption was 4～6 times of medical material amount, and merge extractive liquid, gets the Radix Aconiti Lateralis Preparata extracting solution; Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is passed through macroporous resin respectively, first ethanol elution with 4～6 times of water gagings or 20% following concentration, eluent is abandoned or adopted, reuse adds 1～10 times of eluting of 60～90% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution respectively.

2, getting the people and participate in 60～80% ethanol, is the supersound extraction 3～4 times of 250～800W with ultrasonic power, and each 20～45 minutes, the solvent consumption was 4～6 times of medical material amount, merge extractive liquid,, the Radix Ginseng extractive solution; Getting Radix Aconiti Lateralis Preparata adds water or adds that to be adjusted to pH with concentrated hydrochloric acid be 2～3 sour water, with ultrasonic power is the supersound extraction 3～4 times of 250～800W, each 20～45 minutes, the solvent consumption is 4～6 times of medical material amount, merge extractive liquid,, being concentrated into 50～60 ℃ of relative densities is 1.1～1.3, add ethanol and make concentration of alcohol reach 70%, cold preservation 24～36 hours filters, reclaim ethanol to there not being the alcohol flavor, add ethanol and make concentration of alcohol reach 80%, cold preservation 24～36 hours filters to clarification, reclaim ethanol to there not being the ethanol flavor, get the Radix Aconiti Lateralis Preparata extracting solution; Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is passed through macroporous resin respectively, first ethanol elution with 4～6 times of water gagings or 20% following concentration, eluent is abandoned or adopted, reuse adds 1～10 times of eluting of 60～90% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the attached extract that gives of getting of purification Radix Aconiti Lateralis Preparata extracting solution.

3, getting the people and participate in 60～80% ethanol, is the supersound extraction 3～4 times of 250～800W with ultrasonic power, and each 20～45 minutes, the solvent consumption was 4～6 times of medical material amount, merge extractive liquid,, the Radix Ginseng extractive solution.Get Radix Aconiti Lateralis Preparata and add 60～80% ethanol, reflux, extract, 3-4 times, each 1～2 hour, the solvent consumption was 4～6 times of medical material amount, merge extractive liquid, gets the Radix Aconiti Lateralis Preparata extracting solution.Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is passed through macroporous resin respectively, first ethanol elution with 4～6 times of water gagings or 20% following concentration, eluent is abandoned or adopted, reuse adds 1～10 times of eluting of 60～90% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution.

4, getting the people and participate in 60～80% ethanol, is the supersound extraction 3～4 times of 250～800W with ultrasonic power, and each 20～45 minutes, the solvent consumption was 4～6 times of medical material amount, merge extractive liquid,, the Radix Ginseng extractive solution.Get Radix Aconiti Lateralis Preparata and add water or concentrated hydrochloric acid to regulate pH be 2～3 sour water, decoct and extract each 1～2 hour 3～4 times, the solvent consumption is 4～6 times of medical material amount, merge extractive liquid,, and being concentrated into 50～60 ℃ of relative densities is 1.1～1.3, add ethanol and make concentration of alcohol reach 70%, cold preservation 24～36 hours filters, reclaim ethanol to there not being the alcohol flavor, add ethanol and make concentration of alcohol reach 80%, cold preservation 24～36 hours filters to clarification, reclaim ethanol to there not being the ethanol flavor, get the Radix Aconiti Lateralis Preparata extracting solution; Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is passed through macroporous resin respectively, first ethanol elution with 4～6 times of water gagings or 20% following concentration, eluent is abandoned or adopted, reuse adds 1～10 times of eluting of 60～90% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution.

5, getting the people and participate in water, is the supersound extraction 3～4 times of 250～800W with ultrasonic power, and each 20～45 minutes, the solvent consumption was 4～6 times of medical material amount.Merge extractive liquid,, being concentrated into 50～60 ℃ of relative densities is 1.1～1.3, adds ethanol and makes concentration of alcohol reach 75%, cold preservation 10～48 hours, filter, reclaim ethanol, add ethanol and make concentration of alcohol reach 85% to there not being the alcohol flavor, cold preservation 24～36 hours filters to clarification, gets the Radix Ginseng extractive solution.Getting Radix Aconiti Lateralis Preparata and add 60～80% ethanol, is the supersound extraction 3～4 times of 250～800W with ultrasonic power, each 20～45 minutes, and the solvent consumption? be 4～6 times of the medical material amount, merge extractive liquid, gets the Radix Aconiti Lateralis Preparata extracting solution.Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is passed through macroporous resin respectively, first ethanol elution with 4～6 times of water gagings or 20% following concentration, eluent is abandoned or adopted, reuse adds 1～10 times of eluting of 60～90% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution.

6, getting the people and participate in water, is the supersound extraction 3～4 times of 250～800W with ultrasonic power, and each 20～45 minutes, the solvent consumption was 4～6 times of medical material amount.Merge extractive liquid,, being concentrated into 50～60 ℃ of relative densities is 1.1～1.3, adds ethanol and makes concentration of alcohol reach 75%, cold preservation 10～48 hours, filter, reclaim ethanol, add ethanol and make concentration of alcohol reach 85% to there not being the alcohol flavor, cold preservation 24～36 hours filters to clarification, gets the Radix Ginseng extractive solution.Getting Radix Aconiti Lateralis Preparata adds water or concentrated hydrochloric acid to regulate pH is 2～3 sour water, with ultrasonic power is the supersound extraction 3～4 times of 250～800W, each 20～45 minutes, the solvent consumption is 4～6 times of medical material amount, merge extractive liquid,, being concentrated into 50～60 ℃ of relative densities is 1.1～1.3, adding ethanol makes concentration of alcohol reach 70%, cold preservation 24～36 hours filters, and reclaims ethanol to there not being the alcohol flavor, adding ethanol makes concentration of alcohol reach 80%, cold preservation 24～36 hours filters to clarification, reclaims ethanol to there not being the ethanol flavor, get the Radix Aconiti Lateralis Preparata extracting solution with above-mentioned Radix Ginseng extractive solution, the Radix Aconiti Lateralis Preparata extracting solution filters respectively, filtrate is respectively by macroporous resin, and with the ethanol elution of 4～6 times of water gagings or 20% following concentration, eluent is abandoned or adopted earlier, reuse adds 1～10 times of eluting of 60～90% ethanol, the collection eluent, decompression recycling ethanol gets the purification Radix Ginseng extractive solution to there not being the alcohol flavor, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution.

7, getting the people and participate in water, is the supersound extraction 3～4 times of 250～800W with ultrasonic power, and each 20～45 minutes, the solvent consumption was 4～6 times of medical material amount.Merge extractive liquid,, being concentrated into 50～60 ℃ of relative densities is 1.1～1.3, adds ethanol and makes concentration of alcohol reach 75%, cold preservation 10～48 hours, filter, reclaim ethanol, add ethanol and make concentration of alcohol reach 85% to there not being the alcohol flavor, cold preservation 24～36 hours filters to clarification, gets the Radix Ginseng extractive solution.Get Radix Aconiti Lateralis Preparata and add 60～80% ethanol, reflux, extract, 3-4 times, each 1～2 hour, the solvent consumption was 4～6 times of medical material amount, merge extractive liquid, gets the Radix Aconiti Lateralis Preparata extracting solution.Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is passed through macroporous resin respectively, first ethanol elution with 4～6 times of water gagings or 20% following concentration, eluent is abandoned or adopted, reuse adds 1～10 times of eluting of 60～90% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution.

8, getting the people and participate in water, is the supersound extraction 3～4 times of 250～800W with ultrasonic power, and each 20～45 minutes, the solvent consumption was 4～6 times of medical material amount.Merge extractive liquid,, being concentrated into 50～60 ℃ of relative densities is 1.1～1.3, adds ethanol and makes concentration of alcohol reach 75%, cold preservation 10～48 hours, filter, reclaim ethanol, add ethanol and make concentration of alcohol reach 85% to there not being the alcohol flavor, cold preservation 24～36 hours filters to clarification, gets the Radix Ginseng extractive solution.Getting Radix Aconiti Lateralis Preparata adds water or concentrated hydrochloric acid to regulate pH is 2～3 sour water, decoct and extract 3～4 times, each 1～2 hour, the solvent consumption is 4～6 times of medical material amount, merge extractive liquid,, being concentrated into 50～60 ℃ of relative densities is 1.1～1.3, adding ethanol makes concentration of alcohol reach 70%, cold preservation 24～36 hours filters, and reclaims ethanol to there not being the alcohol flavor, adding ethanol makes concentration of alcohol reach 80%, cold preservation 24～36 hours filters to clarification, reclaims ethanol to there not being the ethanol flavor, get the Radix Aconiti Lateralis Preparata extracting solution with above-mentioned Radix Ginseng extractive solution, the Radix Aconiti Lateralis Preparata extracting solution filters respectively, filtrate is respectively by macroporous resin, and with the ethanol elution of 4～6 times of water gagings or 20% following concentration, eluent is abandoned or adopted earlier, reuse adds 1～10 times of eluting of 60～90% ethanol, the collection eluent, decompression recycling ethanol gets the purification Radix Ginseng extractive solution to there not being the alcohol flavor, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution.

9, get Radix Ginseng water extraction 2-3 times, each 1-2 hours, the solvent consumption was 4～6 times of medical material amount.Merge extractive liquid,, being concentrated into 50～60 ℃ of relative densities is 1.1～1.3, add ethanol and make concentration of alcohol reach 75%, cold preservation 10～48 hours, filtration adds ethanol and makes concentration of alcohol reach 85%, and cold preservation 24～36 hours filters to clarification, gets the Radix Ginseng extractive solution.Getting Radix Aconiti Lateralis Preparata and add 60～80% ethanol, is the supersound extraction 3～4 times of 250～800W with ultrasonic power, and each 20～45 minutes, the solvent consumption was 4～6 times of medical material amount, merge extractive liquid,, the Radix Aconiti Lateralis Preparata extracting solution.Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is passed through macroporous resin respectively, first ethanol elution with 4～6 times of water gagings or 20% following concentration, eluent is abandoned or adopted, reuse adds 1～10 times of eluting of 60～90% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution.

10, get Radix Ginseng water extraction 2-3 times, each 1-2 hours, the solvent consumption was 4～6 times of medical material amount.Merge extractive liquid,, being concentrated into 50～60 ℃ of relative densities is 1.1～1.3, add ethanol and make concentration of alcohol reach 75%, cold preservation 10～48 hours, filtration adds ethanol and makes concentration of alcohol reach 85%, and cold preservation 24～36 hours filters to clarification, gets the Radix Ginseng extractive solution.Getting Radix Aconiti Lateralis Preparata adds water or concentrated hydrochloric acid to regulate pH is 2～3 sour water, with ultrasonic power is the supersound extraction 3～4 times of 250～800W, each 20～45 minutes, the solvent consumption is 4～6 times of medical material amount, merge extractive liquid,, being concentrated into 50～60 ℃ of relative densities is 1.1～1.3, add ethanol and make concentration of alcohol reach 70%, cold preservation 24～36 hours filters, reclaim ethanol to there not being the alcohol flavor, add ethanol and make concentration of alcohol reach 80%, cold preservation 24～36 hours filters to clarification, reclaim ethanol to there not being the ethanol flavor, get the Radix Aconiti Lateralis Preparata extracting solution; Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is passed through macroporous resin respectively, first ethanol elution with 4～6 times of water gagings or 20% following concentration, eluent is abandoned or adopted, reuse adds 1～10 times of eluting of 60～90% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution.

11, get Radix Ginseng water extraction 2-3 times, each 1-2 hours, the solvent consumption was 4～6 times of medical material amount.Merge extractive liquid,, being concentrated into 50～60 ℃ of relative densities is 1.1～1.3, add ethanol and make concentration of alcohol reach 75%, cold preservation 10～48 hours, filtration adds ethanol and makes concentration of alcohol reach 85%, and cold preservation 24～36 hours filters to clarification, gets the Radix Ginseng extractive solution.Get Radix Aconiti Lateralis Preparata and add 60～80% ethanol, reflux, extract, 3-4 times, each 1～2 hour, the solvent consumption was 4～6 times of medical material amount, merge extractive liquid, gets the Radix Aconiti Lateralis Preparata extracting solution.Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is passed through macroporous resin respectively, first ethanol elution with 4～6 times of water gagings or 20% following concentration, eluent is abandoned or adopted, reuse adds 1～10 times of eluting of 60～90% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution.

12, get Radix Ginseng water extraction 2-3 times, each 1-2 hours, the solvent consumption was 4～6 times of medical material amount.Merge extractive liquid,, being concentrated into 50～60 ℃ of relative densities is 1.1～1.3, add ethanol and make concentration of alcohol reach 75%, cold preservation 10～48 hours, filtration adds ethanol and makes concentration of alcohol reach 85%, and cold preservation 24～36 hours filters to clarification, gets the Radix Ginseng extractive solution.Get Radix Aconiti Lateralis Preparata and add water or concentrated hydrochloric acid to regulate pH be 2～3 sour water, decoct and extract each 1～2 hour 3～4 times, the solvent consumption is 4～6 times of medical material amount, merge extractive liquid,, and being concentrated into 50～60 ℃ of relative densities is 1.1～1.3, add ethanol and make concentration of alcohol reach 70%, cold preservation 24～36 hours filters, reclaim ethanol to there not being the alcohol flavor, add ethanol and make concentration of alcohol reach 80%, cold preservation 24～36 hours filters to clarification, reclaim ethanol to there not being the ethanol flavor, get the Radix Aconiti Lateralis Preparata extracting solution; Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is passed through macroporous resin respectively, first ethanol elution with 4～6 times of water gagings or 20% following concentration, eluent is abandoned or adopted, reuse adds 1～10 times of eluting of 60～90% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution.

13, get the people and participate in 60～80% ethanol, reflux, extract, 3～4 times, each 1～2 hour, the solvent consumption was 4～6 times of medical material amount, merge extractive liquid, gets the Radix Ginseng extractive solution.Getting Radix Aconiti Lateralis Preparata and add 60～80% ethanol, is the supersound extraction 3～4 times of 250～800W with ultrasonic power, each 20～45 minutes, and the solvent consumption? be 4～6 times of the medical material amount, merge extractive liquid, gets the Radix Aconiti Lateralis Preparata extracting solution.Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is passed through macroporous resin respectively, first ethanol elution with 4～6 times of water gagings or 20% following concentration, eluent is abandoned or adopted, reuse adds 1～10 times of eluting of 60～90% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution.

14, get the people and participate in 60～80% ethanol, reflux, extract, 3～4 times, each 1～2 hour, the solvent consumption was 4～6 times of medical material amount, merge extractive liquid, gets the Radix Ginseng extractive solution.Getting Radix Aconiti Lateralis Preparata adds water or concentrated hydrochloric acid to regulate pH is 2～3 sour water, with ultrasonic power is the supersound extraction 3～4 times of 250～800W, each 20～45 minutes, the solvent consumption is 4～6 times of medical material amount, merge extractive liquid,, being concentrated into 50～60 ℃ of relative densities is 1.1～1.3, add ethanol and make concentration of alcohol reach 70%, cold preservation 24～36 hours filters, reclaim ethanol to there not being the alcohol flavor, add ethanol and make concentration of alcohol reach 80%, cold preservation 24～36 hours filters to clarification, reclaim ethanol to there not being the ethanol flavor, get the Radix Aconiti Lateralis Preparata extracting solution; Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is passed through macroporous resin respectively, first ethanol elution with 4～6 times of water gagings or 20% following concentration, eluent is abandoned or adopted, reuse adds 1～10 times of eluting of 60～90% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution.

15, get the people and participate in 60～80% ethanol, reflux, extract, 3～4 times, each 1～2 hour, the solvent consumption was 4～6 times of medical material amount, merge extractive liquid, gets the Radix Ginseng extractive solution.Get Radix Aconiti Lateralis Preparata and add water or concentrated hydrochloric acid to regulate pH be 2～3 sour water, decoct and extract each 1～2 hour 3～4 times, the solvent consumption is 4～6 times of medical material amount, merge extractive liquid,, and being concentrated into 50～60 ℃ of relative densities is 1.1～1.3, add ethanol and make concentration of alcohol reach 70%, cold preservation 24～36 hours filters, reclaim ethanol to there not being the alcohol flavor, add ethanol and make concentration of alcohol reach 80%, cold preservation 24～36 hours filters to clarification, reclaim ethanol to there not being the ethanol flavor, get the Radix Aconiti Lateralis Preparata extracting solution; Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is passed through macroporous resin respectively, first ethanol elution with 4～6 times of water gagings or 20% following concentration, eluent is abandoned or adopted, reuse adds 1～10 times of eluting of 60～90% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution.

Above-mentioned macroporous resin column model can be D101, D201, D601, HP-20 etc.

Three, the preparation method of Radix Ginseng, Radix Aconiti Lateralis Preparata extract described in the preparation of the present invention is preferred but be not limited in the following method any one:

1, get the people and participate in 80% ethanol, supersound extraction 4 times, ultrasonic power is 400W, is respectively 30,30,25,25 minutes, and the solvent consumption is 6 times of medical material amount, and merge extractive liquid, reclaims ethanol to there not being the ethanol flavor, gets the Radix Ginseng extractive solution.Get Radix Aconiti Lateralis Preparata and add 80% ethanol, supersound extraction 4 times, ultrasonic power is 400W, is respectively 30,30,25,25 minutes, and the solvent consumption is 6 times of medical material amount, and merge extractive liquid, gets the Radix Aconiti Lateralis Preparata extracting solution.Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is respectively the macroporous resin of D101 by model, earlier with 4 times of water gaging eluting, eluent is abandoned or adopted, reuse adds 5 times of eluting of 65% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution.

2, get the people and participate in 60% ethanol, supersound extraction 3 times, ultrasonic power 500W was respectively 30,30,25 minutes, and the solvent consumption is 4 times of medical material amount, and merge extractive liquid, gets the Radix Ginseng extractive solution.Get Radix Aconiti Lateralis Preparata and add concentrated hydrochloric acid to regulate pH be 2～3 sour water, supersound extraction 3 times, ultrasonic power is 500W, be respectively 30,30,25 minutes, the solvent consumption is 4～6 times of medical material amount, merge extractive liquid,, being concentrated into 60 ℃ of relative densities is 1.25, adds ethanol and makes concentration of alcohol reach 70%, cold preservation 24 hours, filter, reclaim ethanol, add ethanol and make concentration of alcohol reach 80% to there not being the alcohol flavor, cold preservation 24 hours, filtration is reclaimed ethanol to there being the ethanol flavor to clarification, gets the Radix Aconiti Lateralis Preparata extracting solution; Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is respectively the macroporous resin of D601 by model, earlier with 5 times of water gaging eluting, eluent is abandoned or adopted, reuse adds 10 times of eluting of 60% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution.

3, get the people and participate in 70% ethanol, supersound extraction 3 times, ultrasonic power is 500W, is respectively 30,25,25 minutes, and the solvent consumption is 5 times of medical material amount, and merge extractive liquid, gets the Radix Ginseng extractive solution.Get Radix Aconiti Lateralis Preparata and add 70% ethanol, reflux, extract, 4 times was respectively 2,2,1,1 hours, and the solvent consumption is 5 times of medical material amount, and merge extractive liquid, gets the Radix Aconiti Lateralis Preparata extracting solution.Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is the D601 macroporous resin by model respectively, measure 12% ethanol elution with 4 times earlier, eluent is abandoned or adopted, reuse adds 6 times of eluting of 75% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution.

4, get the people and participate in 75% ethanol, supersound extraction 3 times, ultrasonic power is 600W, is respectively 30,25,25 minutes, and the solvent consumption is 6 times of medical material amount, and merge extractive liquid, gets the Radix Ginseng extractive solution.Getting Radix Aconiti Lateralis Preparata, to add pH be 2 the sour water of regulating with concentrated hydrochloric acid, decocts and extract 3 times, was respectively 2,1,1 hours, the solvent consumption is 4.5 times of medical material amount, merge extractive liquid,, and being concentrated into 50 ℃ of relative densities is 1.1, add ethanol and make concentration of alcohol reach 70%, cold preservation 26 hours filters, reclaim ethanol to there not being the alcohol flavor, add ethanol and make concentration of alcohol reach 80%, cold preservation 30 hours filters to clarification, reclaim ethanol to there not being the ethanol flavor, get the Radix Aconiti Lateralis Preparata extracting solution; Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is the HP-20 macroporous resin by model respectively, earlier with 5 times of water gaging eluting, eluent is abandoned or adopted, reuse adds 5 times of eluting of 70% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution.

5, get the people and participate in water, supersound extraction 3 times, ultrasonic power is 700W, is respectively 35,30,20 minutes, the solvent consumption is 5 times of medical material amount.Merge extractive liquid,, being concentrated into 60 ℃ of relative densities is 1.2, add ethanol and make concentration of alcohol reach 75%, cold preservation 24 hours, filtration is reclaimed ethanol and is not distinguished the flavor of to there being alcohol, adds ethanol and makes concentration of alcohol reach 85%, and cold preservation 28 hours filters to clarification, gets the Radix Ginseng extractive solution.Get Radix Aconiti Lateralis Preparata and add 80% ethanol, supersound extraction 3 times, ultrasonic power is 800W, is respectively 25,20,20 minutes, and the solvent consumption is 6 times of medical material amount, and merge extractive liquid, gets the Radix Aconiti Lateralis Preparata extracting solution.Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is the D101 macroporous resin by model respectively, first ethanol elution with 4～6 times of water gagings or 20% following concentration, eluent is abandoned or adopted, reuse adds 2 times of eluting of 85% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution.

6, get the people and participate in water, supersound extraction 3 times, ultrasonic power is 800W, is respectively 30,30,20 minutes, the solvent consumption is 5 times of medical material amount.Merge extractive liquid,, being concentrated into 60 ℃ of relative densities is 1.3, add ethanol and make concentration of alcohol reach 75%, cold preservation 20 hours, filtration is reclaimed ethanol and is not distinguished the flavor of to there being alcohol, adds ethanol and makes concentration of alcohol reach 85%, and cold preservation 30 hours filters to clarification, gets the Radix Ginseng extractive solution.Get Radix Aconiti Lateralis Preparata and add water, supersound extraction 3 times, ultrasonic power is 800W, is respectively 30,30,20 minutes, the solvent consumption was 6 times of medical material amount, merge extractive liquid,, being concentrated into 50 ℃ of relative densities is 1.1, adds ethanol and makes concentration of alcohol reach 70%, cold preservation 30 hours, filter, reclaim ethanol to there not being the alcohol flavor, add ethanol and make concentration of alcohol reach 80%, cold preservation 30 hours, filter to clarification, reclaim ethanol to there not being the ethanol flavor, get the Radix Aconiti Lateralis Preparata extracting solution above-mentioned Radix Ginseng extractive solution, the Radix Aconiti Lateralis Preparata extracting solution filters respectively, and filtrate is the D601 macroporous resin by model respectively, measure 18% ethanol elution with 4 times earlier, eluent is abandoned or adopted, and reuse adds 6 times of eluting of 70% ethanol, collects eluent, decompression recycling ethanol gets the purification Radix Ginseng extractive solution to there not being the alcohol flavor, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution.

7, get the people and participate in water, supersound extraction 3 times, ultrasonic power is 700W, is respectively 30,30,20 minutes, the solvent consumption is 6 times of medical material amount.Merge extractive liquid,, being concentrated into 55 ℃ of relative densities is 1.15, add ethanol and make concentration of alcohol reach 75%, cold preservation 18 hours, filtration is reclaimed ethanol and is not distinguished the flavor of to there being alcohol, adds ethanol and makes concentration of alcohol reach 85%, and cold preservation 30 hours filters to clarification, gets the Radix Ginseng extractive solution.Get Radix Aconiti Lateralis Preparata and add 80% ethanol, reflux, extract, 3 times was respectively 2,1,1 hours, and the solvent consumption is 6 times of medical material amount, and merge extractive liquid, gets the Radix Aconiti Lateralis Preparata extracting solution.Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is the D201 macroporous resin by model respectively, measure 10% ethanol elution with 5 times earlier, eluent is abandoned or adopted, reuse adds 3 times of eluting of 85% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution.

8, get the people and participate in water, supersound extraction 4 times, ultrasonic power is 300W, is respectively 40,40,30,20 minutes, the solvent consumption is 6 times of medical material amount.Merge extractive liquid,, being concentrated into 60 ℃ of relative densities is 1.2, add ethanol and make concentration of alcohol reach 75%, cold preservation 20 hours, filtration is reclaimed ethanol and is not distinguished the flavor of to there being alcohol, adds ethanol and makes concentration of alcohol reach 85%, and cold preservation 30 hours filters to clarification, gets the Radix Ginseng extractive solution.Get Radix Aconiti Lateralis Preparata and add that to adopt concentrated hydrochloric acid to be adjusted to pH be 3 sour water, decoct and extract 3 times, be respectively 2,2,1 hour, the solvent consumption is 4 times of medical material amount, merge extractive liquid,, and being concentrated into 55 ℃ of relative densities is 1.3, adding ethanol makes concentration of alcohol reach 70%, cold preservation 24 hours filters, and reclaims ethanol to there not being the alcohol flavor, adding ethanol makes concentration of alcohol reach 80%, cold preservation 32 hours filters to clarification, reclaims ethanol to there not being the ethanol flavor, get the Radix Aconiti Lateralis Preparata extracting solution with above-mentioned Radix Ginseng extractive solution, the Radix Aconiti Lateralis Preparata extracting solution filters respectively, filtrate is respectively the macroporous resin of D601 by model, and with 6 times of water gaging eluting, eluent is abandoned or adopted earlier, reuse adds 2 times of eluting of 85% ethanol, the collection eluent, decompression recycling ethanol gets the purification Radix Ginseng extractive solution to there not being the alcohol flavor, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution.

9, get Radix Ginseng water extraction 3 times, each 2 hours, the solvent consumption was 4 times of medical material amount.Merge extractive liquid,, being concentrated into 60 ℃ of relative densities is 1.3, add ethanol and make concentration of alcohol reach 75%, cold preservation 19 hours, filtration adds ethanol and makes concentration of alcohol reach 85%, and cold preservation 28 hours filters to clarification, gets the Radix Ginseng extractive solution.Get Radix Aconiti Lateralis Preparata and add 80% ethanol, supersound extraction 3 times, ultrasonic power is 700W, is respectively 30,30,20 minutes, and the solvent consumption is 5.5 times of medical material amount, and merge extractive liquid, gets the Radix Aconiti Lateralis Preparata extracting solution.Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is the D601 macroporous resin by model respectively, earlier with 6 times of water gaging eluting, eluent is abandoned or adopted, reuse adds 8 times of eluting of 65% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution.

10, get Radix Ginseng water extraction 2 times, each 2 hours, the solvent consumption was 5 times of medical material amount.Merge extractive liquid,, being concentrated into 55 ℃ of relative densities is 1.25, add ethanol and make concentration of alcohol reach 75%, cold preservation 17 hours, filtration adds ethanol and makes concentration of alcohol reach 85%, and cold preservation 34 hours filters to clarification, gets the Radix Ginseng extractive solution.Get Radix Aconiti Lateralis Preparata and add water, supersound extraction 3 times, ultrasonic power is 750W, each 30,25,20 minutes, the solvent consumption was 6 times of medical material amount, merge extractive liquid,, being concentrated into 55 ℃ of relative densities is 1.15, adds ethanol and makes concentration of alcohol reach 70%, cold preservation 36 hours, filter, reclaim ethanol, add ethanol and make concentration of alcohol reach 80% to there not being the alcohol flavor, cold preservation 30 hours, filtration is reclaimed ethanol to there being the ethanol flavor to clarification, gets the Radix Aconiti Lateralis Preparata extracting solution; Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is the D601 macroporous resin by model respectively, measure 12% ethanol elution with 4 times earlier, eluent is abandoned or adopted, reuse adds 4 times of eluting of 75% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution.

11, get Radix Ginseng water extraction 3 times, be respectively 2,2,1 hours, the solvent consumption is 6 times of medical material amount.Merge extractive liquid,, being concentrated into 60 ℃ of relative densities is 1.1, add ethanol and make concentration of alcohol reach 75%, cold preservation 28 hours, filtration adds ethanol and makes concentration of alcohol reach 85%, and cold preservation 30 hours filters to clarification, gets the Radix Ginseng extractive solution.Get Radix Aconiti Lateralis Preparata and add 75% ethanol, reflux, extract, 3 times, each 1.5 hours, the solvent consumption was 5 times of medical material amount, merge extractive liquid, gets the Radix Aconiti Lateralis Preparata extracting solution.Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is the D101 macroporous resin by model respectively, first ethanol elution with 4～6 times of water gagings or 20% following concentration, eluent is abandoned or adopted, reuse adds 1～10 times of eluting of 60～90% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution.

12, get Radix Ginseng water extraction 3 times, be respectively 2,2,1 hours, the solvent consumption is 6 times of medical material amount.Merge extractive liquid,, being concentrated into 50 ℃ of relative densities is 1.15, add ethanol and make concentration of alcohol reach 75%, cold preservation 35 hours, filtration adds ethanol and makes concentration of alcohol reach 85%, and cold preservation 26 hours filters to clarification, gets the Radix Ginseng extractive solution.Get Radix Aconiti Lateralis Preparata and add the sour water that adopts concentrated hydrochloric acid to be adjusted to pH=3, decoct and extract 3 times, be respectively 2,1,1 hours, the solvent consumption is 6 times of medical material amount, merge extractive liquid,, and being concentrated into 60 ℃ of relative densities is 1.3, add ethanol and make concentration of alcohol reach 70%, cold preservation 30 hours filters, reclaim ethanol to there not being the alcohol flavor, add ethanol and make concentration of alcohol reach 80%, cold preservation 32 hours filters to clarification, reclaim ethanol to there not being the ethanol flavor, get the Radix Aconiti Lateralis Preparata extracting solution; Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is the HP-20 macroporous resin by model respectively, earlier with 5 times of water gaging eluting, eluent is abandoned or adopted, reuse adds 4 times of eluting of 70% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution.

13, get the people and participate in 60% ethanol, reflux, extract, 4 times, each 1.5 hours, the solvent consumption was 6 times of medical material amount, merge extractive liquid, gets the Radix Ginseng extractive solution.Get Radix Aconiti Lateralis Preparata and add 75% ethanol, supersound extraction 3 times, ultrasonic power is 600W, is respectively 40,30,30 minutes, and the solvent consumption is 6 times of medical material amount, and merge extractive liquid, gets the Radix Aconiti Lateralis Preparata extracting solution.Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is the D201 macroporous resin by model respectively, earlier with 6 times of water gaging eluting, eluent is abandoned or adopted, reuse adds 5 times of eluting of 85% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution.

14, get the people and participate in 80% ethanol, reflux, extract, 3 times was respectively 2,1,1 hours, and the solvent consumption is 4 times of medical material amount, and merge extractive liquid, gets the Radix Ginseng extractive solution.Get Radix Aconiti Lateralis Preparata and add water, supersound extraction 3 times, ultrasonic power is 800W, be respectively 35,30,20 minutes, the solvent consumption is 6 times of medical material amount, merge extractive liquid,, being concentrated into 60 ℃ of relative densities is 1.3, adds ethanol and makes concentration of alcohol reach 70%, cold preservation 24 hours, filter, reclaim ethanol, add ethanol and make concentration of alcohol reach 80% to there not being the alcohol flavor, cold preservation 26 hours, filtration is reclaimed ethanol to there being the ethanol flavor to clarification, gets the Radix Aconiti Lateralis Preparata extracting solution; Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is the D601 macroporous resin by model respectively, measure 15% ethanol elution with 6 times earlier, eluent is abandoned or adopted, reuse adds 9 times of eluting of 65% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution.

15, get the people and participate in 65% ethanol, reflux, extract, 4 times, each 2 hours, the solvent consumption was 6 times of medical material amount, merge extractive liquid, gets the Radix Ginseng extractive solution.Get Radix Aconiti Lateralis Preparata and add the sour water that adopts concentrated hydrochloric acid to be adjusted to pH=2.5, decoct and extract each 1.5 hours 3 times, the solvent consumption is 5 times of medical material amount, merge extractive liquid,, and being concentrated into 60 ℃ of relative densities is 1.25, add ethanol and make concentration of alcohol reach 70%, cold preservation 26 hours filters, reclaim ethanol to there not being the alcohol flavor, add ethanol and make concentration of alcohol reach 80%, cold preservation 34 hours filters to clarification, reclaim ethanol to there not being the ethanol flavor, get the Radix Aconiti Lateralis Preparata extracting solution; Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is the HP-20 macroporous resin by model respectively, earlier with 6 times of ethanol elutions of measuring 18% following concentration, eluent is abandoned or adopted, reuse adds 7 times of eluting of 75% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution.

Described Radix Ginseng of preparation of the present invention or Radix Aconiti Lateralis Preparata extract are the Radix Ginseng of the purification that obtains according to said method or Radix Ginseng extract or the Radix Aconiti Lateralis Preparata extract powder that the Radix Aconiti Lateralis Preparata extracting solution is made by lyophilization or spray drying, the relative density of medicine liquid of spraying is 1.1～1.2 (60～70 ℃), and containing amount of solid is 30～60%; 130～180 ℃ of drying tower inlet temperature, leaving air temp is 90～110 ℃ at the bottom of the tower.Thickness is no more than 10～20mm in the cryodesiccated container, and 12～14 hours freezing time (30～-35 ℃), taking out container, to put into freezer dryer drying time be 20～26 hours.'Shenfu ' of the present invention can select to use the direct preparation of extracting solution of method for preparing according to the needs of dosage form, also can make clinical corresponding preparation by selective extraction thing powder.

Four, preparation of the present invention is except that being raw material directly feeds intake from extract, can also be the raw material preparation that directly feeds intake by raw medicinal material, is that raw material directly feeds intake and prepares the method for joining attached extracting solution and be by raw medicinal material:

1, get Radix Ginseng 50-200 weight portion, Radix Aconiti Lateralis Preparata 100-400 weight portion, add 60～80% ethanol extraction 2～3 times, each ethanol consumption is 4～8 times of medical material amount, extracts 1～2 hour at every turn, and merge extractive liquid, reclaims ethanol to there not being the alcohol flavor, promptly gets and joins attached extracting solution; The attached extracting solution of above-mentioned ginseng is filtered, filtrate is passed through macroporous resin, first ethanol elution with 4～6 times of water gagings or 20% following concentration, eluent is abandoned or adopted, reuse adds 1～10 times of eluting of 50～80% ethanol, collects eluent, and decompression recycling ethanol is to there not being the alcohol flavor, get the purification ginseng and conform to extract, must join after the drying to conform to and carry thing.

2, get Radix Ginseng 50-200 weight portion, Radix Aconiti Lateralis Preparata 100-400 weight portion, supersound extraction 1～3 time, ultrasonic power is 250～800W, each 20～45 minutes, the ethanol consumption was 4～6 times of medical material amount.Merge extractive liquid,, merge extractive liquid, reclaims ethanol to there not being the alcohol flavor, promptly gets and joins attached extracting solution.The attached extracting solution of above-mentioned ginseng is filtered, and filtrate is passed through macroporous resin, first ethanol elution with 4～6 times of water gagings or 20% following concentration, eluent is abandoned or adopted, and reuse adds 1～10 times of eluting of 50～80% ethanol, collects eluent, decompression recycling ethanol gets the purification ginseng and conforms to extract to there not being the alcohol flavor.

Described macroporous resin column model can be D101, D201, D601, HP-20 etc.The attached extracting solution of described ginseng can directly be made liquid preparation, also can make fixedly preparation after drying.

Five, preparation of the present invention is that directly the feed intake method for optimizing of the attached extracting solution of preparation ginseng of raw material is by raw medicinal material:

L, get Radix Ginseng 60-150 weight portion, Radix Aconiti Lateralis Preparata 120-280 weight portion, add 70% ethanol extraction 3 times, each ethanol consumption is 4,6,8 times of medical material amount, extracts 2/1/1 hour at every turn, and merge extractive liquid, reclaims ethanol to there not being the alcohol flavor, promptly gets and joins attached extracting solution.The attached extracting solution of above-mentioned ginseng is filtered, and filtrate is the D101 macroporous resin by model, and with the ethanol elution of 20% following concentration, eluent is abandoned or adopted earlier, and reuse adds 4 times of eluting of 60% ethanol, and collection eluent, decompression recycling ethanol get the purification ginseng and conform to extract to there not being the alcohol flavor.

2, get Radix Ginseng 60-150 weight portion, Radix Aconiti Lateralis Preparata 120-280 weight portion, supersound extraction 2 times, ultrasonic power is 500W, each 30 minutes, the solvent consumption was 5 times of medical material amount.Merge extractive liquid, reclaims ethanol to there not being the alcohol flavor, promptly gets and joins attached extracting solution.The attached extracting solution of above-mentioned ginseng is filtered, and filtrate is the D201 macroporous resin by model, abandons or adopts with 5 times of water gaging eluents earlier, and reuse adds 5 times of eluting of 65% ethanol, and collection eluent, decompression recycling ethanol get the purification ginseng and conform to extract to there not being the alcohol flavor.

Six, preparation of the present invention can be prepared into following dosage form:

1, joins attached drop pill

Press the extract material proportional quantity, with Radix Ginseng extract, Radix Aconiti Lateralis Preparata extract is pulverized, and mix homogeneously is stand-by; Press medicated powder: substrate 1:1-2 with substrate PEG6000 or PEG4000 fusion after, it is even to add medicament mixed, 60-90 ℃ of insulations added in the methyl-silicone oil with 20-70/minutes, collected drop pill, absorbed liquid coolant with filter paper, made drop pill.

2, join attached capsule:

Press the extract material proportional quantity with Radix Ginseng extract, Radix Aconiti Lateralis Preparata extract is pulverized mix homogeneously, adds 1-3 times of adjuvants, makes capsule.

3, join attached granule

Press the extract material proportional quantity with Radix Ginseng extract, the Radix Aconiti Lateralis Preparata extract mix homogeneously adds the adjuvant of 2-5 times of amounts of suitable medicine, makes soft material with the ethanol of 60-90% concentration, makes granule.

4, join attached spray

It is stand-by with Radix Ginseng extract and Radix Aconiti Lateralis Preparata extract mix homogeneously to press the extract material proportional quantity, add 10～20% tween 80 alcoholic solution, 30～300 parts by volume, add 1,2-propylene glycol 100～500 parts by volume, mixing, ethanol are settled to 200～1000 parts by volume g/ml, are sub-packed in the aerosol pressure bottle by 20-100ml (specification), in every bottle, be pressed into dichlorodifluoromethane 1～10 weight portion behind the gland, make spray.

5, join attached oral liquid

Press the extract material proportional quantity with Radix Ginseng extract and Radix Aconiti Lateralis Preparata extract mix homogeneously, adding 0.1-0.3 times of amount of stevioside (g/ml) adds the injection water again and makes oral liquid.

6, SHENFU ZHUSHEYE (common)

Press the extract material proportional quantity with Radix Ginseng extract and Radix Aconiti Lateralis Preparata extract (liquid) mix homogeneously, add 0.2% tween 80, add water for injection to 1000 parts by volume (g/ml), regulate pH to 5～7 with sodium hydroxide, filter embedding, sterilization promptly gets SHENFU ZHUSHEYE.

7, join attached freeze-dried powder

Thickness is no more than 10～20mm in the cryodesiccated container, and 12～14 hours freezing time (30～-35 ℃), taking out container, to put into freezer dryer drying time be 20～26 hours.

8, ginseng attached powder pin (common)

Above-mentioned purification ginseng is conformed to extract or purification Radix Ginseng extractive solution, and purification Radix Aconiti Lateralis Preparata extracting solution adopts the attached injectable powder of spray drying preparation ginseng: the relative density of medicine liquid of spraying is 1.1～1.2 (60～70 ℃), and containing amount of solid is 30～60%; 130～180 ℃ of drying tower inlet temperature, leaving air temp is 90～110 ℃ at the bottom of the tower.

Or the attached injectable powder of lyophilization preparation ginseng: thickness is no more than 10～20mm in the exsiccant container, and 12～14 hours freezing time (30～-35 ℃), taking out container, to put into freezer dryer drying time be 20～26 hours.Lyophilization is prepared into Radix Ginseng extract powder, Radix Aconiti Lateralis Preparata extract powder, and after the mixing, packing under the aseptic condition promptly gets and joins attached injectable powder.

9, join attached infusion solutions

Press the extract material proportional quantity with Radix Ginseng extract and Radix Aconiti Lateralis Preparata extract mix homogeneously, add water for injection to dissolving, add tween 80 again, add water for injection to 1500-2600 parts by volume (g/ml), regulate pH to 5～7, filter with sodium hydroxide, adding NaCL accent etc. oozes, embedding, sterilization promptly gets and joins attached infusion solutions.

10, the attached high concentration injection of ginseng

Above-mentioned purification ginseng is conformed to extract be concentrated into 1-5 times of the former degree of depth; Or purification Radix Ginseng extractive solution and purification Radix Aconiti Lateralis Preparata extracting solution be concentrated into 1～5 times of original content respectively, merges; Transfer PH5-7 fill, sterilization promptly gets the high concentration SHENFU ZHUSHEYE.Method for concentration is: vacuum concentration, vacuum are 0.08～0.9MPa, 40～80 ℃ of temperature.The SHENFU ZHUSHEYE of making is 1～5 times of SHENFU ZHUSHEYE concentration commonly used, contains the crude drug amount that is equivalent in promptly every ml high concentration injection: Radix Ginseng 0.1～0.5g, Radix Aconiti Lateralis Preparata 0.2～1g.

11, ginseng aconite injection tablet

(1) above-mentioned purification ginseng is conformed to extract and adopt spray drying or the attached fine powder of lyophilization preparation ginseng.

(2) adopt spray drying or lyophilization to be prepared into Radix Ginseng extract powder, Radix Aconiti Lateralis Preparata extract powder respectively above-mentioned purification Radix Ginseng extractive solution, purification Radix Aconiti Lateralis Preparata extracting solution; Radix Ginseng extract powder, Radix Aconiti Lateralis Preparata extract powder mixes.

After above fine powder all can be added suitable adjuvant (mannitol or lactose, or the two mixes with certain proportion) respectively, the aseptic condition lower sheeting promptly got and faces with the preceding ginseng aconite injection tablet of preparation as required.Sheet heavily is 0.15～0.5g.

The weight proportion of each component is during the preparation tablet: ginseng conforms to carries fine powder: mannitol 1:0.2～2

The mixture of Radix Ginseng extract powder and Radix Aconiti Lateralis Preparata extract powder: mannitol=1:0.2～2

Mixed accessories be mannitol: lactose=1:0.1～1

The people participates in the Radix Aconiti Lateralis Preparata extracting solution and merges the attached fine powder of ginseng that spray drying or lyophilization preparation are adopted in the back: mannitol=1:0.2～2

The people participates in the Radix Aconiti Lateralis Preparata extracting solution and merges the attached fine powder of ginseng that spray drying or lyophilization preparation are adopted in the back: lactose=1:0.2～2

The people participates in the Radix Aconiti Lateralis Preparata extracting solution and merges the attached fine powder of ginseng that spray drying or lyophilization preparation are adopted in the back: mixed accessories=1:0.2～2.

12. tablet

Press the extract material proportional quantity with Radix Ginseng extract and Radix Aconiti Lateralis Preparata extract mix homogeneously, add an amount of magnesium stearate, mix, tabletting, bag film-coat.

13. rapid release drop pill

Press the extract material proportional quantity with Radix Ginseng extract and Radix Aconiti Lateralis Preparata extract mix homogeneously, join in the substrate that is made into the ratio of 3-5:1-2 by Polyethylene Glycol (PEC) 6000 and lecithin (PC), extract mixtures and substrate ratio are 1:2-4, it is liquid to be heated to 60-90 ℃ of one-tenth, splashes into 20-70/minutes speed and is cooled to ball 20-100mg/ ball in the liquid Paraffin.

14, skeleton type sustained release preparation

Skeleton generally is divided into: insoluble (as ethyl cellulose＜EC 〉, polyethylene, polypropylene, polysiloxanes, ethylene-vinyl acetate copolymer, polymethyl methacrylates etc.) the waxiness skeleton is (as fat, Wax, stearic acid, stearyl alcohol, glyceryl monostearate, Brazil wax, octadecanols etc.) hydrophilic gel is (as CMC, CMC-Na, MC, PVA, HEC, SCMC, sodium alginate, pectin, alginate, agar, hydroxypropyl emthylcellulose＜HPMC 〉, chitosan, chitin, galactomannan etc.)

(1) insoluble matrix tablet:

Directly will join attached extract and insoluble framework material (ethyl cellulose, polymethyl methacrylate) mix homogeneously, compacting in flakes.

(2) waxiness matrix tablet:

Directly will join attached extract and framework material (stearic acid, stearyl alcohol, glyceryl monostearate, Brazil wax) and pulverize, mix homogeneously is granulated dry tablet forming afterwards after making soft material with an amount of ethanol.

To join attached extract and wax material (stearic acid, stearyl alcohol, glyceryl monostearate, Brazil wax) puts in the blender, heating makes into the pastille skeleton particle, or with adding after the fusion of low melting point waxiness in the blender in the medicated powder of preheating, stir and form medicine-containing particle, compacting is in flakes.

(3) hydrogel matrix tablet:

To join attached extract and mix with it, and granulate after making soft material with small amount of ethanol, dry back adds fluidizer (octadecane fumaric acid), tabletting.

Attached extract and hydrophilic gel (1% sodium alginate soln, 4%～9% HPMC, 0.65%～11.8% chitosan soln, PVA) be will join and dry granulation, granulate, tabletting mixed.

(4) Entogastric lingering sheet

Material: hydrophilic colloid PHMC, HPC, HEC, MC, EC, SCMC, PVP and PVA use in conjunction formulation method:

To join attached extract and pulverize, and with 2%HPMC aqueous solution system soft material, sieve, 40 ℃ of dry granulate add tabletting behind the magnesium stearate mixing.

To join attached extract and SCMC and pulverize, sieve, mix direct powder compression.

Join attached extract and be binding agent with 5%EC alcoholic solution (swell in 95% medicinal alcohol in), the system soft material sieves, and drying adds lubricant, sieves.Mixing, tabletting.

To join attached extract and mix, granulate with starch slurry with PVP, PVA, drying, granulate adds small amount of N aHCO ₃, after the magnesium stearate, compacting is in flakes.

15, sustained release coating preparation

Coating membrane mostly is cellulose acetate, ethyl cellulose (EC) and methacrylic acid copolymer.

Antiplastering aid is Pulvis Talci, magnesium stearate, silicon dioxide, titanium dioxide etc., and consumption is 1～3% of a coating solution volume.

(1) film controlled release small pieces

Coating material mostly is the sustained release coating material such as the silicone elastomer of cellulose acetate, ethyl cellulose (EC) and some no permeance properties.

Label is made granule for the attached extract of ginseng with gelatin or 5%SCMC slurry, is pressed into the small pieces of diameter 3～5mm, with fluidisation nebulization coating or fluidized bed coating coating, the coated pellets of some is packed in the hard capsule.

(2) microporous membrane coated tablet

Coating material: water-soluble substanceses such as the material of pore-forming agents such as cellulose acetate, ethyl cellulose, ethylene-acetate ethylene copolymer, polyacrylic resin such as PEG class, PVP, PVA, sodium lauryl sulphate, sugar and salt also can add some water-insoluble powder such as Pulvis Talci, silicon dioxide etc.

Ginseng attached extract and conventional adjuvant are pressed into the label that diameter is 11mm, and coating is promptly to the coating pan with label.

(3) pilule

A. film controlled piller: membrane material is: hydrophilic film clothing, insoluble film-coat (polyacrylic resin class, cellulose acetate, ethyl cellulose etc.), microporous membrane coating (ethyl cellulose, cellulose acetate, polydimethylsiloxane etc.)

B. matrix type piller: framework material: the derivant of glyceryl monostearate, stearic acid, stearyl alcohol, castor oil hydrogenated, Cera Flava, Brazil wax and ethyl cellulose, cellulose acetate butyrate, polyethylene-acetate ethylene copolymer and polymethacrylates, microcrystalline Cellulose, hydroxypropyl cellulose etc.

Adjuvant: can add 10%～15% lactose

To join attached extract and adjuvant mix homogeneously, the solution that adds entry or PVP, PVA, HPMC, SCMC etc. is as coagulating agent or binding agent, powder made have certain plastic moistening uniform material, makes piller with extruding-round as a ball one-tenth ball method, drying, promptly.

Extract of the present invention is joined attached each extract by the suitable approximately raw medicinal herbs 14-50 of the every weight portion of amount of solid weight portion because of extracting method difference and the rate of extract difference; The contained extract amount of each preparation consumption per day can be by quite total crude drug amount 20-40 gram.The present invention's experiment is carried out pharmacodynamic study with variant 'Shenfu ' respectively, each oral formulations of the present invention and ejection preparation be contrived experiment according to a conventional method all, each Pharmacodynamic test of active extract result shows: each preparation medicine of the present invention all has treatment myocardial ischemia, arrhythmia, rise blood pressure, the non-special resistance of enhancing body, improves effect such as cardiac function.The used experimental example of this description is example with the injection type, and following experimental example only is used to further specify the present invention, but can not be as any limitation of the invention.

The influence of the hemodynamic parameter of 1 pair of myocardial ischemia dog of experimental example

1, experiment material and method:

1.1 experimental drug: pentobarbital sodium, chemical pure, chemical plant packing product is known in import packing, last sea-run, lot number 921019,25g/ is bottled.

1.2 laboratory animal: the hybrid dog is buied after Huaxi Medical Univ's Experimental Animal Center fed for 1 week by market, and is used for experiment when not having disease on inspection.

1.3 experimental apparatus: Japan's product eight road physiology monitors (RM-6000, NIHONKOHDEN), Japanese electrogenesis magnetic flowmeter (NIHONKOHDEN), pressure transducer (P231D, Gould, LIS), respirator (KTH-2 type, Shaoxing three or five instrucment and meter plant's products).

1.4 test methods and procedures: 12 male dogs, 11-13kg is divided into two groups at random, 6 every group.First group of matched group gives normal saline 0.5ml/kg in the experiment, join attached group for second group, gives SHENFU ZHUSHEYE 0.5ml/kg in the experiment.The capable general anesthesia of intraperitoneal injection pentobarbital 30ml/kg, endotracheal intubation connects the capable mechanical ventilation of respirator.Connect cardiac diagnosis lead and write down cardiac output in animal foot.The 4th intercostal space is opened breast and is exposed heart in the left side, separates root of ascending aorta, and Motionless electromagnetic effusion meter control head (016mm) record cardiac output.Insert cardiac catheter in left ventricular apex portion, by pressure transducer record ventricular pressure and left constant pressure differential (dp/dt).Separate left femoral artery, intubate recording blood pressure, electrocardiogram, cardiac output, left constant pressure and (dp/dt) monitor by eight road physiology monitors records and fluorescent screen at last, it is worth as the control value before the ligation coronary artery.

In crown moving fat anterior descending branch 1/2 place ligation, cause myocardial ischemia.After the ligation 5 minutes, the record These parameters.Then, by the left femoral vein administration.Administration is write down These parameters in the time of 30 minutes once more.

Use VO ₂=(heart rate * mean pressure) ^1/2Calculating myocardium oxygen consumption: use formula: TPR (the cloudy power of periphery)=MAP (mean pressure)/CO (cardiac output), the TPR of computation cycles.With formula MW (per minute work done)=(MAP-6) * CO * 13.6/1000, calculate cardiac work.

1.5 statistical result: each each parameter value of organizing is added up into average and standard deviation: the t check analysis statistical significance of the difference between group: organize before and after the interior ligation and the difference of medication paired t-test analytical statistics meaning.

Table 1: SHENFU ZHUSHEYE is to the cardiac muscle effect of myocardial ischemia dog, oxygen consumption and hemodynamic influence

The grouped record heart rate SBP DBP MAP LVP CO dp/dt TPR VO2 MW n=10 time, (beats/min), (mmHg), (mmHg), (mmHg), (mmHg) L/min mmHg/s, (mmHg.min/L) teats.mmHg/min, (Kg.m)

158±7.4 134±4.9 93±5.2 108±5.1 311±5.4 0.99±0.05 2778±139 109±6.7 131±6..7 1.45±0.08 5 172±4.4* 102±12.6* 69±3.8* 80±8.5 98±12.5* 0.70±0.03* 1360±3538* 114±5.5 117±7.5* 0.70±0.06* 5 173±6.0 97±11.7 64±4.1* 75±8.2 94±11.6 0.67±0.04* 1308±348 112±5.8 114±5.9* 0.63±0.05* 159±6.3 133±5.8 95±3.6 107±4.7 132±4.5 0.98±0.05 2830±179 108±3.9 130±5.6 1.38±0.09 5 173±4.3* 104±7.6 69±5.8* 81±6.2* 102±10.3 0.68±0.04* 1175±307* 119±4.8 118±3.7* 0.69±0.06* 5 151±2.7#a 121±7.6*#a 76±2.2*#a 91±3.5*#a 19±7.4*#a 0.83±0.05*#a 2030±223*#a 110±3.2# 117±4.1* 0.959±0.07*#a

2, result

Experimental result is summarized in the table 1.From expression formula as seen, after giving SHENFU ZHUSHEYE treatment and handling, the cardiac function of myocardial ischemia animal be improved significantly, show that mainly blood pressure gos up, cardiac output increases, the work done of heart per minute improves; And matched group gives normal saline preceding no significant difference.

Experimental example 2 SHENFU ZHUSHEYE are to ARR influence

1, experiment material and method

1.1 experimental drug: pentobarbital sodium, chemical pure, chemical plant packing product is known in import packing, last sea-run, lot number 921019,25g/ is bottled.Aconitine, German E.Merck company produces and produces, every bottle of 250mg dress.Propranolol, Changzhou the 4th pharmaceutical Co. Ltd produces and produces.

1.2 laboratory animal: the hybrid male dogs, buy use after Huaxi Medical Univ's Experimental Animal Center fed for 1 week by market.The SD rat is provided by Huaxi Medical Univ's Experimental Animal Center breeding nursing.

1.3 experimental apparatus: Japan's product eight road physiology monitors (RM-6000, NIHONKOHDEN), respirator (KTH-2 type, Shaoxing three or five instrucment and meter plant's products).

1.4 test methods and procedures:

Modelling of myocardial ischemia arrhythmia and drug effect:

12 dogs, 10-13kg is divided into two groups at random, and first group is matched group, gives normal saline 0.5ml/kg in the experiment, joins attached group for second group, gives SHENFU ZHUSHEYE 0.5ml/kg in the experiment.The capable general anesthesia of intraperitoneal injection pentobarbital 30ml/kg.Fixedly animal is on operating-table, and endotracheal intubation connects respirator pedestrian worker and breathes.Separate left strand artery and vein, tremulous pulse is inserted the monitoring blood pressure in tremulous pulse, and venous cannulation and slow dropping liquid (8-10/minutes of normal saline) keep vein unimpeded, use during in order to administration.Fixedly ECG electrode is in the contrast as ligation coronary artery medicine of animal foot and recording ecg.Open breast in left breast the 4th intercostal space, expose heart, before left coronary artery, fall the 1/2 place ligation coronary artery of branch then, cause myocardial ischemia to change.

After the ligation 1 minute, when promptly the electrocardio of animal changes, write down the contrast of 5 minutes electrocardiograms before as administration, then by above-mentioned dosage intravenous administration, administration in the time of 30 minutes recording ecg make electrocardiogram after the medicine administration.Add up the variation of heart rate respectively, per 5 minutes premature beat occurrence numbers, indoor tachycardia etc., the variation medication paired t-test of group before and after the innerlich anwenden analyzed the statistical significance of its difference, the difference t check analysis statistical significance between group.

The effect of medicine arrhythmia and SHENFU ZHUSHEYE

20 SD rats, 260-300g is divided into two groups at random, and first group gives normal saline 0.5ml/kg, and second group gives SHENFU ZHUSHEYE 0.5ml/kg.Intraperitoneal injection pentobarbital (30ml/kg) anesthetized rat fixedly animal separates the left femoral vein intubate and is used for infused drug on laboratory table, be used for infused drug at the tail venous cannulation, is used for the infusion aconitine at tail vein insert head sword-shaped needle.After all were ready, first group of intravenous pushed away normal saline 0.5ml/kg, the second treated animal intravenous injection SHENFU ZHUSHEYE 0.5ml/kg.After the administration 5 minutes, with moving pump morning from tail vein 5ug/kg/min constant speed injection aconitine, till heart beating stops jumping.Eight road physiology monitors record and fluorescent screen monitor the different Electrocardiographic variation of II, write down ventricular premature contraction (VE) to occur the aconitine consumption when ventricular tachycardia rate (VT), fibrillation aroused in interest (VF) and cardiac arrest (CA).

The black No.1 alkali consumption of VE, VT, VF and CA takes place in each group of statistical analysis when the No.1 alkali of venous transfusion crow, the aconitine consumption difference size between each group is carried out statistical disposition with t-check.

The effect of medicine bradycardia and SHENFU ZHUSHEYE: 12 dogs, 10-13kg is divided into two groups at random, and first group is matched group, gives normal saline 0.5ml/kg in the experiment, joins attached group for second group, gives SHENFU ZHUSHEYE 0.5ml/kg in the experiment.Intraperitoneal injection pentobarbital (30ml/kg) anesthetized dog.Fixedly animal separates left femoral vein and intubate and is used for infused drug on operating-table.After ready, write down the animal electrocardiograms as normal control with eight road physiology.After finishing, by duodenum, propranolol 120mg/kg, after 30 minutes, the opening entry electrocardiogram is as the preceding contrast of treatment.Give propranolol in the time of 35 minutes, the opening entry electrocardiogram is as the treatment processing costs.

Each treated animal of statistical analysis is given the forward and backward changes in heart rate of propranolol, and treat the changes in heart rate after the reason, on the same group in the forward and backward and treatment of administration when handling heart rate difference analyze its statistical significance with paired t-test, its statistical significance of group difference t check analysis.

2 experimental results

2.1 SHENFU ZHUSHEYE is to the ARR effect of myocardial ischemia

SHENFU ZHUSHEYE includes VE and VT to the arrhythmia that myocardial ischemia causes, improves significantly, and experimental result shows and is summarized in the table 1.Electrocardio by dog behind the visible ligation coronary artery of table 1 changes, and by having VE and VT in the past VE and VT does not take place, give SHENFU ZHUSHEYE after, VE and VT obviously descend than matched group.

Table 1 SHENFU ZHUSHEYE lacks the influence (X ± S) of sedan-chair arrhythmia to dog cardiac muscle

Group detects heart rate premature beat ventricular tachycardia (n=6) time (inferior/5 minute) (inferior/5 minute) (inferior/5 minute)
	Front 157.5 ± 7.4 0.6 ± 0.7 0.0 ± 0.0 control groups of ischemic { front 158.0 ± 6.0 0.8 ± 0.9 0.0 ± 0.0 Shenfu groups of ischemic 3 minutes 171.8 ± 4.4 38.0 ± 4.5 35.2 ± 4.8 ischemics 5 minutes 172.0 ± 6.0 41.2 ± 4.3 39.6 ± 5.2 ischemics { 5 fens 151.7 ± 2.7#a, 11.2 ± 2.7*#a of ischemic 3 minutes 172.8 ± 4.3 39.3 ± 5.2 37.2 ± 3.9 ischemics, 9.1 ± 2.5*#a

* before P＜0.05 and the ischemia on the same group relatively, #P＜0.05 and contrast with time value relatively, aP＜0.05 and group ischemia 3 minutes are relatively.

Be also shown in by table 1, give SHENFU ZHUSHEYE treatment and make the heart rate due to the ischemia accelerate to be reduced effectively.

2.2 SHENFU ZHUSHEYE is to the arrhythogenic influence of aconitine

After giving animal infusion aconitine, VE, VT, VF have appearred in animal, and further CA appears in infusion aconitine animal, but the animal that has given SHENFU ZHUSHEYE in advance the above-mentioned ARR time occurs than normal saline group evening, be that the aconitine consumption is big than matched group, see Table 2.

Table 2 SHENFU ZHUSHEYE is to the influence of rat ventricular due to the aconitine (X ± S)

Group aconitine consumption (ug/kg) is VE VT VF CA (n=6)
	Matched group 33 ± 2.3 40 ± 3.6 51 ± attached group of 38+4.1 47 ± 5.3 58 ± 5.8 103 ± 7.5 of 4.4 94 ± 6.9 ginsengs

* compare with matched group P＜0.05

2.3 SHENFU ZHUSHEYE is analysed influence to dog bradycardia due to the propranolol

Animal heart rate after giving propranolol is slack-off significantly, is slowed to for more than 160 time more than 90 time by normal per minute, sees Table 3.After giving ginseng aconite injection, the heart rate that slows down has quickening, though fail to return to normal level.

Table 3 ginseng is attached to the influence of the delaying (X ± S) that move of the dog heart due to the propranolol

Group gives HR (n=6) after the reagent (inferior/minute) (inferior/minute) (inferior/minute) for behind the propranolol HR for before the propranolol HR
	Matched group 158 ± 17.5 93 ± 8.2*, 95 ± 8.8* joins attached group 161 ± 19.1 96 ± 7.3*, 126 ± 15.1*#a

* P＜0.05 with give propranolol on the same group before HR relatively, #P＜0.05 with give propranolol on the same group after HR relatively, P＜0.05 and the matched group HR same period are relatively

By this result of study, show that SHENFU ZHUSHEYE in the arrhythmia that the treatment myocardial ischemia causes, comprises that ventricular premature contraction, ventricular tachycardia and heart rate quickening etc. have good therapeutic effect; For medicine, as aconitine etc., arrhythmia that causes such as ventricular premature contraction and ventricular tachycardia have certain antagonism in addition; The bradycardia that causes for propranolol has some improvement.Prove also that by a systematic study SHENFU ZHUSHEYE endeavoured the slow dual regulation that has for tachycardia and the heart.Therefore SHENFU ZHUSHEYE can be widely used in common clinically arrhythmia, and cardiac function decline is accompanied with cardiovascular disease such as arrhythmia.

The influence of 3 pairs of different pathological animal patterns of experimental example blood pressure

1 material and method

1.1 experimental drug: pentobarbital sodium, chemical pure, chemical plant packing product is known in import packing, last sea-run, lot number 921019,25g/ is bottled.Aconitine, German E.Merck company produces and produces, every bottle of 25mg dress.

1.2 laboratory animal: the hybrid male dogs, buy and use after Huaxi Medical Univ's Experimental Animal Center is fed a week by market.The SD rat is provided by Huaxi Medical Univ's Experimental Animal Center.

1.3 experimental apparatus: the eight road physiology monitors that Japanese photoelectricity company produces (RM-6000, NIHONKOHDEN), pressure transducer (P23ID, Gouid, CA, USA).Respirator (KTH-2 type, Shaoxing three or five instrument and meter factories are produced).

1.4 experimental procedure and method

SHENFU ZHUSHEYE is to the influence of constriction ventral aorta rat blood pressure.

20 male SD rats, 260-300g is divided into two groups at random, 10 every group.Opening abdomen and expose aorta under the sterilization aseptic condition, is standard constriction ventral aorta with No. 6 needle points.When postoperative is fed 15 days, separate left femoral artery intubate recording blood pressure.After finishing, first group is intraperitoneal injection normal saline 0.4ml/kg, every day secondary, the second treated animal intraperitoneal injection SHENFU ZHUSHEYE 0.4ml/kg, every day secondary.When continuing 15 days by this administration, intraperitoneal injection pentobarbital sodium anesthetized animal separates right femoral artery and intubate record arteriotony.

SHENFU ZHUSHEYE is to the influence of normal rat blood pressure

20 male SD rats, 260-300g is divided into two groups at random, 10 every group.First group is intraperitoneal injection normal saline 0.4ml/kg, every day secondary, the second treated animal intraperitoneal injection SHENFU ZHUSHEYE 0.4ml/kg, every day secondary.When continuing 15 days by this administration, intraperitoneal injection pentobarbital sodium 30ml/kg anesthetized animal separates left femoral artery and intubate record arteriotony.

SHENFU ZHUSHEYE is to the influence of the blood pressure of myocardial ischemia dog

12 male dogs, 11---13kg is divided into two groups, 6 every group at random.Intraperitoneal injection pentobarbital sodium 30ml/kg anesthetized animal, fixedly animal is on operating-table, and the circulation of qi promoting cannula connects the capable mechanical ventilation of respirator.Separate left femoral artery and vein, the former causes the preceding contrast of curing myocardial ischemia processing with the pressure transducer place of linking ligation arteria coronaria by arterial cannulation.Then, injecting normal saline 0.5ml/kg in the first treated animal femoral vein, the second treated animal intraperitoneal injection SHENFU ZHUSHEYE 0.5ml/kg.Administration is in the time of 30 minutes, recording blood pressure.

1.5 statistical disposition

Each organizes blood pressure, and systolic pressure and diastolic pressure are added up and be average and standard deviation; Difference between group its statistical significance of t-check analysis.

2 experimental results

2.1 join the influence of attached blood pressure to Hypertensive Rats

Experimental result is summarized in the table 1, by table as seen, be slightly larger than clinical consumption SHENFU ZHUSHEYE and handle after the Hypertensive Rats and the matched group that gives normal saline there is no the difference of significance; The equal value height of the diastolic pressure of attached group of matched group and ginseng when feeding 30 days, but zero difference between group than 15 days.

Table 1 SHENFU ZHUSHEYE is to the influence of the blood pressure of Hypertensive Rats (X ± S)

Grouping SBO (mmHg) DBP (mmHg) (n=10) 15-30 days 15-30 days
	Matched group 176 ± 15.9 185 ± 16.2 129 ± 9.6 148 ± 14.2 ginsengs attached group 172 ± 16.3 188 ± 18.6 127 ± 10.3 152 ± 16.7

2.2 SHENFU ZHUSHEYE is to the influence of normal rat blood pressure

Give normal rat continuous injection ginseng aconite injection after emerging January the blood pressure of rat do not have the significance difference, see Table 2.

Table 2 SHENFU ZHUSHEYE is to the influence of normal rat blood pressure (X ± S)

Grouping (n=10) SBO (mmHg) DBP (mmHg)
	Matched group 151 ± 12.8 116 ± 10.1 ginsengs attached group 154 ± 13.4 112 ± 11.7

2.3 SHENFU ZHUSHEYE is to the influence of myocardial ischemia dog blood pressure

Behind the coronary artery of ligation dog, the blood pressure of dog obviously descends, and sees Table 3.After giving ginseng aconite injection, the blood pressure of dog significantly gos up than matched group, sees Table 3

Table 3 SHENFU ZHUSHEYE is to the influence of the blood pressure of myocardial ischemia dog (X ± S)

Grouping SBO (mmHg) DBP (mmHg) is (n=6) before the ligation after the ligation after the administration before the ligation after the ligation after the administration
	Matched group 134 ± 4.9 102 ± 12.6 97 ± 11.7* 93 ± 5.2 69 ± attached group 133 ± 5.9 104 ± 7.6* of 3.8 64 ± 4.9 ginsengs 121 ± 4.7*#a 94 ± 3.8 68 ± 5.8* 76 ± 2.2*#a

Show by this experimental result, influence shows that mainly blood pressure falls when the normal level situation to SHENFU ZHUSHEYE to blood pressure regulating, thereby descending as myocardial contraction due to the myocardial ischemia causes this class cardiovascular disease that blood pressure reduces, and it mainly regulates effect is that blood pressure is gone up.

The non-special resistance effect of experimental example 4 enhancing body

1 material

1.1 trial drug

The ginsenoside, pale yellow powder, self-control, content 98% (calculating) with the panoxadiol.

1.2 animal

Kunming kind white mice body weight 18-22g, all except that special circumstances were indicated in literary composition, female, male half and half animal was all adopted in all tests.SD rat body weight 150-180g, male and female half and half.All animals provide by Sichuan Province's antibiotics industrial research institute animal center, the laboratory animal quality certification number: the real moving Guan Zhidi in river No. 85 (mice) and real moving No. 92, the Guan Zhidi (rat) in river.

1.3 it is commercially available that reagent isoproterenol, aspirin are.

2 methods and result

2.1 anti-refrigeration test

70 of mices, male and female half and half, divide 7 groups at random, iv medicine or normal saline every day 1 time, continuous 5 days, heavy h behind the last medicine, respectively each group mice is put wire mesh cage and is built in the refrigerator-freezer, refrigerator-freezer temperature-6 ℃ is observed the mice active situation, stop to be decided to be mouse diing time more than 1 minute with the mouse breathing activity, the results are shown in Table 1.

Table 1 ginseng attached liquid is to the influence of freezing induced mice death (X ± S)

Freezing death time of dosage Mus number postpones death time P value medicine (/Kg) (only) (h) (%)
	Physiological saline 10ml 10 32.6 ± 7.4 Shenfu liquid 5ml 10 44.3 ± 5.9 35.9＜0.01 10ml 10 46.2 ± 6.1 42.7＜0.01 20ml 10 52.5 ± 7.9 61.0＜0.01 ginseng wheat liquid 10ml, 10 44.2 ± 7.6 35.6＜0.05 ginsenoside 200mg (ig) 10 46.6 ± 6.5 42.9＜0.01

By table 1 as seen, the ginseng attached liquid has significant protective effect to freezing induced mice death, and Rhizoma Zingiberis Recens liquid iV and ginsenoside ig also have obvious effect, but action intensity is like a little less than.

2.2 high temperature resistance test

Mice and grouping administration are all with 2.1, and 1h behind the last medicine puts mice in people's wire mesh cage, inserts in the baking oven again; Temperature is 550 ± 1 ℃ in two baking ovens, observes to be found in that mice is movable in the thermal environment significantly increases, jump, mouth, the nose flow liquid, crouch down then or lie on one's side, dead condition sees Table 2.

Table 2 ginseng attached liquid is to the influence of high temperature induced mice death (X ± S)

The dosage Mus is counted the high temperature death time and postpones death time P value medicine (/Kg) (only) (h) (%)
	Physiological saline 10ml 10 15.2 ± 3.7 Shenfu liquid 5ml 10 17.3 ± 6.5 13.8〉0.05 10ml 10 21.4 ± 2.5 40.8＜0.05 20ml 10 22.2 ± 2.8 46.1＜0.05 ginseng wheat liquid 10ml, 10 24.2 ± 3.6 60.5＜0.01 ginsenoside 200mg (ig) 10 22.7 ± 3.2 49.3＜0.05

By table 2 as seen, death still has certain protective role to the ginseng attached liquid to the high temperature induced mice, and Rhizoma Zingiberis Recens liquid also has protective effect, but effect is strong slightly.

2.3 influence to the mice hypoxia-bearing capability

Mice, grouping and administration are all with 1h behind the 2.1 last medicines, and also lid is tight in the 250ml wide-mouth port grinding bottle of Sheng sodica calx in one by one mice being inserted, and observe the time of mice because of anoxia asphyxia death, the results are shown in Table 3.

Table 3 ginseng attached liquid is to the influence of mice normal pressure hypoxia-bearing capability (X ± S)

The dosage Mus count hypoxia death time P value medicine (/Kg) (only) (h)
	Physiological saline 10ml 10 22.7 ± 4.2 Shenfu liquid 5ml 10 27.8 ± 4.6〉0.05 10ml 10 30.9 ± 4.8＜0.05 20ml 10 30.2 ± 3.2＜0.05 ginseng wheat liquid 10ml 10 31.1 ± 3.5＜0.01 ginsenoside 200mg (ig) 10 33.9 ± 4.6＜0.01

By table 3 as seen, the ginseng attached liquid can significantly strengthen mice normal pressure hypoxia-bearing capability, and Rhizoma Zingiberis Recens liquid and ginsenoside also have remarkable protective effect.

2.4 the influence of mice hypoxia-bearing capability when isoproterenol is loaded

Operation such as mice, grouping and administration all with the worker with 2.3, but after myrrh, respectively organized ip in mice isoproterenol 20mg/kg in 45 minutes, the 15min rear is packed mice into airtight in the port grinding bottle again, the results are shown in Table 4.

The influence of mice hypoxia-bearing capability under table 4 ginseng attached liquid is loaded to isoproterenol (X ± S)

The dosage Mus count mouse diing time P value medicine (/Kg) (only) (h)
	Physiological saline 10ml 10 14.32 ± 2.15 Shenfu liquid 5ml 10 19.27 ± 2.25＜0.05 10ml 10 24.35 ± 4.69＜0.01 20ml 10 28.65 ± 7.26＜0.01 ginseng wheat liquid 10ml 10 28.26 ± 4.82＜0.01 ginsenoside 200mg (ig) 10 27.61 ± 5.53＜0.01

By table 4 as seen, to strengthen the mice of cardiac muscle load, the ginseng attached liquid still has better protective effect to the injection isoproterenol, and the ginseng attached liquid also has remarkable protection effect, but effect is only strong slightly.

2.5 influence to the mouth breathing of mice broken end

Mice, grouping and administration be with 2.1,1h behind the last medicine, and every at the appointed time Mus is cut off cervical region with profit under cranium, and record is cut off the time that the cervical region mice carries out mouth breathing, the results are shown in Table 5.

Table 5 ginseng attached liquid is to the influence of mice anoxia enduring tolerance (X ± S)

The dosage Mus count mouse diing time P value medicine (/Kg) (only) (h)
	Physiological saline 10ml 10 2.15 ± 0.22 Shenfu liquid 5ml 10 3.32 ± 0.55＜0.05 10ml 10 3.65 ± 0.35＜0.01 20ml 10 3.88 ± 0.44＜0.01 ginseng wheat liquid 10ml 10 3.44 ± 0.28＜0.05 ginsenoside 200mg (ig) 10 3.68 ± 0.35＜0.01

By table 5 as seen, for big cerebral anoxia, ginseng attached liquid and Rhizoma Zingiberis Recens liquid also all have obvious protective effect.

2.6 influence to HAC induced mice writhing response

60 male and female half and half of mice are divided into 6 groups at random, and every day, iv medicine or normal saline were 1 time, and continuous 5 days, 1h behind the last medicine, every Mus ip0.7%HAC0.2ml observes the number of times that writhing response takes place for mice in 20 minutes, the results are shown in Table 6.

Table 6 ginseng attached liquid is to the influence of HAC induced mice writhing response (X ± S)

Dosage Mus number is turned round body number of times suppression ratio P value medicine (/Kg) (only) (h) (%)
	Physiological saline 10ml 10 36.4 ± 7.2 Shenfu liquid 5ml 10 30.6 ± 4.5 15.93〉0.05 10ml 10 22.7 ± 6.2 37.64＜0.05 20ml 10 18.8 ± 5.1 48.35＜0.01 ginseng wheat liquid 10ml, 10 33.9 ± 6.5 6.87〉0.05 ginsenoside 200mg (ig) 10 14.4 ± 3.2 60.44＜0.01

By table 6 as seen, the ginseng attached liquid has obvious inhibitory action to HAC induced mice writhing response, and prompting has certain analgesic activity. but the ginseng attached liquid does not see that HAC induced mice writhing response has a significant effect.

2.7 the influence of the induced mice ear inflammation of xylol

60 of mices, divide 6 groups at random, difference iv medicine or normal saline, production-supply-marketing every day 1, continuous 4 days, 1h behind the last medicine, with the right Mus ear of special syringe needle both sides coating dimethylbenzene 0.02ml, mice is put to death behind the 1h, cut left and right sides auricle, pressing maneuver claims auricle heavily to manage, and as Mus swelling degree, the results are shown in Table 7. with the difference (mg) of auris dextra one left ear weight

The influence of table 7 ginseng attached liquid xylol induced mice ear inflammation (X ± S)

Dosage Mus number is turned round body number of times suppression ratio P value medicine (/Kg) (only) (h) (%)
	Physiological saline 10ml 10 16.3 ± 3.6 Shenfu liquid 5ml 10 12.2 ± 4.1 25.15＜0.05 10ml 10 10.4 ± 2.8 36.20＜0.05 20ml 10 9.6 ± 3.1 41.10＜0.01 ginseng wheat liquid 10ml, 10 12.0 ± 2.5 26.38＜0.05 ginsenoside 200mg (ig) 10 8.2 ± 1.8 49.70＜0.01

By table 7 as seen, Mus otitis disease has obvious inhibitory action due to the ginseng attached liquid xylol, but action intensity is little.

2.8 influence to Mus otitis disease due to the adrenalectomy mice dimethylbenzene

70 male and female half and half of mice are divided 7 groups at random, difference iv medicine show or normal saline, and every day 1 time, continuous 4 days, tested the 3rd day, except that the 1st, 5 group, all the other 2-4 and 6,7 groups are all excised bilateral adrenal gland, silk suture from the back otch under the ether light anaesthesia.1h behind the last medicine causes inflammation with dimethylbenzene to two of mouse right ears with 2.7 tests, measures Mus ear swelling degree behind the 1h, the results are shown in Table 8.

Table 8 ginseng attached liquid is to the influence of Mus ear swelling due to the adrenalectomy mice dimethylbenzene (x ± s)

Group drug dose Mus count normal mice adrenalectomy Mus ear weightening finish P value (/Kg) (only) (h) (%) and 1 group compare and 2 groups of ratios
	1 physiological saline 10ml, 10 √ 14.2 ± 2.8 2 physiological saline 10ml, 10 √ 18.2 ± 6.2＜0.05 3 Shenfu liquid 5ml, 10 √ 14.1 ± 3.3〉0.05 4 Shenfu liquid 10ml, 10 √ 12.2 ± 3.1〉0.05 5 Shenfu liquid 20ml, 10 √ 9.8 ± 2.5＜0.05＜0.05 6 ginseng wheat liquid 10ml, 10 √ 10.1 ± 3.1＜0.05＜0.05 7 aspirin 200mg (ig), 10 √ 9.3 ± 3.1＜0.05＜0.05

n＝10

By table 8 as seen, attain under scorching the stimulation inflammatory reaction of adrenalectomy mice more normally for strong at equality strength.The ginseng attached liquid has remarkable inhibitory action to the dimethylbenzene inflammation of normal mouse, weakens but the adrenalectomy mice then acted on, and shows that the above-mentioned agency part of joining attached liquid depends on adrenal complete existence.

Experimental example 5 Antishock functions

1 material

1.1 trial drug

Dexamethasone sodium phosphate injection, 5mg/ml props up, and Xiangfan, Hubei pharmaceutcal corporation, Ltd of Hang Seng produces, lot number: 990206.

1.2 animal

Kunming kind white mice body weight 18-22g, all except that special circumstances were indicated in literary composition, female, male half and half animal was all adopted in all tests.SD rat body weight 150-180g, male and female half and half.All animals provide by Huaxi Medical Univ's Experimental Animal Center.The laboratory animal quality certification number: the real moving Guan Zhidi in river No. 67 (mice) and real moving No. 70, the Guan Zhidi (rat) in river.

1.3 reagent

Escherichia coli (O ₁₁₁B ₄) endotoxin, Sigma produces; D-galactosamine, chemistry teaching and research room of Medical University Of Chongqing provides; Actinomycin D.

2 methods and result

2.1 influence to the endotoxin shock death

120 of mices are divided into 6 groups at random, and iv administration every day 1 time, continuous 5 days, drug withdrawal 24h attacked with escherichia coli endotoxin iV, and dosage 25mg/kg observes the mice shock death situation behind the endotoxin that gives, and the results are shown in Table 1.

Table 1 SHENFU ZHUSHEYE is to the influence of endotoxin induced mice shock death

The dead Mus of drug dose test Mus number count the P value (/kg) (only) (min)
	Physiological saline 20ml 20 18 Shenfu liquid 5ml 20 12＜0.05 Shenfu liquid 10ml 20 10＜0.05 Shenfu liquid 20ml 20 4＜0.01 ginseng wheat liquid 10ml 20 8＜0.05 dexamethasone 10mg 20 1＜0.01

By table 1 as seen, the death of the internal toxin attacks induced mice of ginseng attached liquid has significant protective effect.

2.2 influence to D-galactosamine sensitization mice endotoxin shock death

140 of mices are divided 7 groups at random, and every day, difference iv medicine or normal saline were 1 time, continuous 5 days.10h behind medicine on the 4th, except that the normal control group, the equal ipD of all the other 6 groups of mices-galactosamine 600mg/kg, 1h behind the medicine on the 5th, each organizes the equal iv escherichia coli endotoxin of little oxygen 0.2mg/kg, observes the dead mouse situation, the results are shown in Table 2.

Table 2 ginseng attached liquid is to the influence of D-galactosamine sensitization mice endotoxin shock death

The dead Mus of drug dose D-galactosamine test Mus number count the P value (/kg) (only) (min)
	Physiological saline 10ml-20 0＜0.01 physiological saline 10ml 600 20 19 Shenfu liquid 5ml 600 20 8＜0.05 Shenfu liquid 10ml 600 20 7＜0.01 Shenfu liquid 20ml 600 20 2＜0.01 ginseng wheat liquid 10ml 600 20 6＜0.01 dexamethasone 10mg 600 20 8＜0.01

By table 2 as seen, the sensitivity of D-internal toxin attacks of galactosamine hepatic injury mice greatly improves, and the endotoxin of 200ug/kg is several to cause whole dead mouses.The ginseng attached liquid has good protective action to the interior coprime shock death of hepatic injury mice due to the galactosamine, can reduce the shock death percentage rate significantly, and be certain dose-effect relationship.

2.3 influence to the death of lambda line rhzomorph D damage mice endotoxin shock

140 male and female half and half of mice are divided 7 groups at random, every day iv medicine or normal saline, continuous 5 days of every day 1 time, 1h behind the last medicine is except that the normal control group, every Mus lumbar injection 0.5mg/kg actinomycin D and 0.5mg/kg escherichia coli endotoxin mixed liquor are observed the dead mouse situation, the results are shown in Table 3.

Table 3 ginseng attached liquid is to the influence of actinomycin D damage mice endotoxin shock death

The dead Mus of drug dose actinomycin D endotoxin test Mus number count the P value (/kg) (mg/kg) (mg/kg) (only) (min)
	Physiological saline 10ml---20 0＜0.01 physiological saline 10ml, 0.5 0.5 20 20 Shenfu liquid 5ml, 0.5 0.5 20 18〉0.05 Shenfu liquid 10ml, 0.5 0.5 20 14〉0.05 Shenfu liquid 20ml, 0.5 0.5 20 8＜0.01 ginseng wheat liquid 10ml, 0.5 0.5 20 9＜0.01 dexamethasone 10mg 0.5 0.5 20 2＜0.01

By table 3 as seen, actinomycetes D damage mice greatly increases endotoxic sensitivity, and the endotoxin of 0.5mg/kg can cause and all be tried dead mouse.The ginseng attached liquid also has significant protective effect to the endotoxin shock death of actinomycin D enhanced sensitivity mice.

2.4 influence to the plain shock death of adrenalectomy

140 of mices are divided 7 groups at random, and iv gives medicine or normal saline, production-supply-marketing every day 1, continuous 5 days, 1h behind administration medicine on the 3rd, each Mus ip pentobarbital sodium 35mg/kg anaesthetizes, extract the bilateral adrenal gland from the back otch, the adrenal gland is not extractd in the only capable sham-operation of normal control group.1h behind the last medicine, each Mus iv escherichia coli endotoxin 0.2mg/kg, dead mouse situation in the 48h the results are shown in Table 4 behind the observation counteracting toxic substances.

Table 4 ginseng attached liquid is to the influence of adrenalectomy mice endotoxin shock death

Drug dose extract the adrenal gland test the dead Mus of Mus number count the P value (/kg) (only) (min)
	Physiological saline 10ml-20 0＜0.01 physiological saline 10ml √ 20 14 Shenfu liquid 5ml √ 20 6＜0.05 Shenfu liquid 10ml √ 20 3＜0.01 Shenfu liquid 20ml √ 20 4＜0.01 ginseng wheat liquid 10ml √ 20 5＜0.01 dexamethasone 10mg √ 20 0＜0.01

As can be seen from Table 4, mice is being raising to endotoxic sensitivity behind the adrenalectomize, and the escherichia coli endotoxin of 0.2mg/kg can cause half mice shock death.The ginseng attached liquid also has significant protective effect to adrenalectomy mice shock death.

2.5 influence to rat shock death

60 of rats, male and female half and half are divided 6 groups at random, difference iv medicine or normal saline, every day 1 time, continuous 5 days, 1h behind the last medicine, the equal iv escherichia coli endotoxin of each Mus 20mg/kg observes the rat shock situation, and record rats death number the results are shown in Table 5.

Table 5 ginseng attached liquid to rat shock death influence (X ± S)

The dosage Mus count the death time dead Mus count medicine (/Kg) (only) (h)
	Physiological saline 10ml 10 12.14 ± 3.61 10 Shenfu liquid 5ml, 10 18.24 ± 4.22 8 10ml 10 24.52 ± 7.86** 6* 20ml, 10 38.55 ± 11.78** 2** ginseng wheat liquid 10ml, 10 22.17 ± 4.86** 6* dexamethasone 10mg, 10-0**

Compare * P＜0.05, * * P＜0.01 with the normal saline group

Endotoxin is a main cause of infective shock, other shock, closing folder property shock etc. as hemorrhagic shock, traumatic shock, superior mesenteric artery also all has the endotoxin factor to participate in, and the above-mentioned often shock of serious endotoxemia is to the key factor of irreversible deterioration.This paper studies the Antishock function of understanding the ginseng attached liquid; the result shows that the ginseng attached liquid has good protective action to endotoxin shock, no matter be to the intact animal, or the sensitization animal; the ginseng attached liquid all has good result, shows clinical main treatment of multiple shock conduct or the auxiliary treatment of can be used for of ginseng attached liquid.

Following embodiment is used to further specify the present invention and all can realizes above-mentioned pharmacological effect.

Embodiment 1Join attached drop pill

Radix Ginseng 140g, Radix Aconiti Lateralis Preparata 280g add 70% ethanol extraction 3 times, and each ethanol consumption is 8/6/4 times of medical material amount, extract 2/1/1 hour at every turn, and merge extractive liquid, reclaims ethanol to there not being the alcohol flavor, promptly gets and joins attached extracting solution.The attached extracting solution of above-mentioned ginseng is filtered, filtrate is by macroporous resin (model is D101), and with the ethanol elution of 20% following concentration, eluent is abandoned or adopted earlier, reuse adds 4 times of eluting of 60% ethanol, the collection eluent, decompression recycling ethanol gets purification and joins attached extracting solution to there not being the alcohol flavor, spray drying, adopt membrane separation technique further refining, the model made from extra care used film is FLT-Z (II)---FLT-VL, gets Radix Ginseng, Radix Aconiti Lateralis Preparata closes carries thing 12.6g, press medicated powder: substrate 1: 1 is with after the substrate PEG6000 fusion, it is even to add medicament mixed, amounts to 31.5g, 75 ℃ of insulations, add in the methyl-silicone oil with 50 droplets/minute, collect drop pill, absorb liquid coolant, make drop pill with filter paper.Totally 720 balls, the heavy 35mg of every ball, consumption per day 48 balls.One time 24 ball, 2 times/day.

Embodiment 2Join attached capsule:

Radix Ginseng 150g, Radix Aconiti Lateralis Preparata 300g add 70% ethanol extraction 3 times, and each ethanol consumption is 8/6/4 times of medical material amount, extract 2/1/1 hour at every turn, and merge extractive liquid, reclaims ethanol to there not being the alcohol flavor, promptly gets and joins attached extracting solution.The attached extracting solution of above-mentioned ginseng is filtered, filtrate is by macroporous resin (model is D101), first ethanol elution with 20% following concentration, eluent is abandoned or adopted, reuse adds 4 times of eluting of 60% ethanol, collects eluent, and decompression recycling ethanol is to there not being the alcohol flavor, get purification and join attached extracting solution, spray drying adopts membrane separation technique further refining, and the model made from extra care used film is FLT-Z (II)---FLT-VG---FLT-VAB---FLT-VAC, Radix Ginseng, Radix Aconiti Lateralis Preparata close and carry thing 18g, add the dextrin of 15.75g, make 135 of capsules, each 3, day obeys 3 times, the 0.25g/ grain.

Embodiment 3Join attached granule

Radix Ginseng 130g, Radix Aconiti Lateralis Preparata 200g add 70% ethanol extraction 3 times, and each ethanol consumption is 8/6/4 times of medical material amount, extract 2/1/1 hour at every turn, and merge extractive liquid, reclaims ethanol to there not being the alcohol flavor, promptly gets and joins attached extracting solution.The attached extracting solution of above-mentioned ginseng is filtered, filtrate is by macroporous resin (model is D101), first ethanol elution with 20% following concentration, eluent is abandoned or adopted, reuse adds 4 times of eluting of 60% ethanol, collects eluent, and decompression recycling ethanol is to there not being the alcohol flavor, get purification and join attached extracting solution, spray drying adopts membrane separation technique further refining, and the model made from extra care used film is FLT-VL, Radix Ginseng, Radix Aconiti Lateralis Preparata close and carry thing 13.2g, add the dextrin of 3 times of amounts of suitable medicine, make soft material, make granule 53g with the ethanol of 60% concentration, each 5g, day clothes 1 time.

Embodiment 4Join attached spray

Radix Ginseng 120g, Radix Aconiti Lateralis Preparata 260g add 70% ethanol extraction 3 times, and each ethanol consumption is 8/6/4 times of medical material amount, extract 2/1/1 hour at every turn, and merge extractive liquid, reclaims ethanol to there not being the alcohol flavor, promptly gets and joins attached extracting solution.The attached extracting solution of above-mentioned ginseng is filtered, and filtrate is by macroporous resin (model is D101), first ethanol elution with 20% following concentration, eluent is abandoned or adopted, reuse adds 4 times of eluting of 60% ethanol, collects eluent, and decompression recycling ethanol is to there not being the alcohol flavor, get purification and join attached extracting solution, spray drying adopts membrane separation technique further refining, and the model made from extra care used film is FLT-VL, Radix Ginseng, Radix Aconiti Lateralis Preparata close and carry thing 15.2g, Radix Ginseng, Radix Aconiti Lateralis Preparata are closed to carry thing fine powder mix homogeneously stand-by; Add the 20% glycerol 150ml that joins, dissolve moisturizing to 300ml, mixing, 50ml or 20ml or 30ml/ bottle are sub-packed in the aerosol pressure bottle, are pressed into dichlorodifluoromethane 2g behind the gland in every bottle, make spray, daily 6-8ml.

Embodiment 5Join attached oral liquid

Radix Ginseng 150g, Radix Aconiti Lateralis Preparata 290g add 70% ethanol extraction 3 times, and each ethanol consumption is 8/6/4 times of medical material amount, extract 2/1/1 hour at every turn, and merge extractive liquid, reclaims ethanol to there not being the alcohol flavor, promptly gets and joins attached extracting solution.The attached extracting solution of above-mentioned ginseng is filtered, filtrate is by macroporous resin (model is D101), and with the ethanol elution of 20% following concentration, eluent is abandoned or adopted earlier, reuse adds 4 times of eluting of 60% ethanol, the collection eluent, decompression recycling ethanol gets purification and joins attached extracting solution to there not being the alcohol flavor, lyophilization, Radix Ginseng, Radix Aconiti Lateralis Preparata close and carry thing 17.6g, add the stevioside of 0.03g, reprocessing skill water is made the 280ml oral liquid.

Embodiment 6SHENFU ZHUSHEYE

Radix Ginseng 110g, Radix Aconiti Lateralis Preparata 220g add 70% ethanol extraction 3 times, and each ethanol consumption is 8/6/4 times of medical material amount, extract 2/1/1 hour at every turn, and merge extractive liquid, reclaims ethanol to there not being the alcohol flavor, promptly gets and joins attached extracting solution.The attached extracting solution of above-mentioned ginseng is filtered, and filtrate is by macroporous resin (model is D101), first ethanol elution with 20% following concentration, eluent is abandoned or adopted, and reuse adds 4 times of eluting of 60% ethanol, collects eluent, decompression recycling ethanol gets purification and joins attached extracting solution, spray drying to there not being the alcohol flavor, Radix Ginseng, Radix Aconiti Lateralis Preparata close and carry thing 13.2g, add 0.2% tween 80, add water for injection to 1100ml, regulate pH to 6 with sodium hydroxide, filter embedding, sterilization promptly gets SHENFU ZHUSHEYE.

Embodiment 7Join attached freeze-dried powder

Radix Ginseng 110g, Radix Aconiti Lateralis Preparata 220g add 70% ethanol extraction 3 times, and each ethanol consumption is 8/6/4 times of medical material amount, extract 2/1/1 hour at every turn, and merge extractive liquid, reclaims ethanol to there not being the alcohol flavor, promptly gets and joins attached extracting solution.The attached extracting solution of above-mentioned ginseng is filtered, filtrate is by macroporous resin (model is D101), first ethanol elution with 20% following concentration, eluent is abandoned or adopted, reuse adds 4 times of eluting of 60% ethanol, collect eluent, decompression recycling ethanol gets purification and joins attached extracting solution to there not being the alcohol flavor, carries out lyophilization, thickness is no more than 15mm in exsiccant container,-32 ℃ of freezings 13 hours are taken out container and were put into freezer dryer dry 24 hours, are prepared into Radix Ginseng, Radix Aconiti Lateralis Preparata extract powder, packing under the aseptic condition promptly gets Shenfu freeze driet powder injection 0.2g/ bottle.

Embodiment 8Join attached powder pin

Radix Ginseng 110g, Radix Aconiti Lateralis Preparata 220g add 70% ethanol extraction 3 times, and each ethanol consumption is 8/6/4 times of medical material amount, extract 2/1/1 hour at every turn, and merge extractive liquid, reclaims ethanol to there not being the alcohol flavor, promptly gets and joins attached extracting solution.The attached extracting solution of above-mentioned ginseng is filtered, filtrate is by macroporous resin (model is D101), with the ethanol elution of 20% following concentration, eluent is abandoned or adopted earlier, and reuse adds 4 times of eluting of 60% ethanol, collect eluent, decompression recycling ethanol gets purification and joins attached extracting solution to there not being the alcohol flavor, carries out spray drying, 160 ℃ of drying tower inlet temperature, leaving air temp is 100 ℃ at the bottom of the tower.The relative density of medicine liquid of spraying is 1.15 (65 ℃), and containing amount of solid is 45%, is prepared into the ginseng, prepared aconite root extract powder, and packing under aseptic condition promptly gets and joins attached injectable powder 0.2g/ bottle.

Embodiment 9Join attached infusion solutions

Radix Ginseng 110g, Radix Aconiti Lateralis Preparata 220g add 70% ethanol extraction 3 times, and each ethanol consumption is 8/6/4 times of medical material amount, extract 2/1/1 hour at every turn, and merge extractive liquid, reclaims ethanol to there not being the alcohol flavor, promptly gets and joins attached extracting solution.The attached extracting solution of above-mentioned ginseng is filtered, filtrate is by macroporous resin (model is D101), with the ethanol elution of 20% following concentration, eluent is abandoned or adopted earlier, and reuse adds 4 times of eluting of 60% ethanol, collect eluent, decompression recycling ethanol gets purification and joins attached extracting solution, lyophilization to there not being the alcohol flavor, Radix Ginseng, Radix Aconiti Lateralis Preparata close and carry thing 13.2g, the tween 80 of adding 0.2% is added water for injection to 2750ml, regulates pH to 6 with sodium hydroxide, filter, add NaCL accent etc. and ooze, embedding, sterilization, promptly get and join attached infusion solutions, 250ml/ days.

Embodiment 10Join attached high concentration injection

Radix Ginseng 110g, Radix Aconiti Lateralis Preparata 220g add 70% ethanol extraction 3 times, and each ethanol consumption is 8/6/4 times of medical material amount, extract 2/1/1 hour at every turn, and merge extractive liquid, reclaims ethanol to there not being the alcohol flavor, promptly gets and joins attached extracting solution.The attached extracting solution of above-mentioned ginseng is filtered, and filtrate is by macroporous resin (model is D101), first ethanol elution with 20% following concentration, eluent is abandoned or adopted, and reuse adds 4 times of eluting of 60% ethanol, collects eluent, decompression recycling ethanol gets purification and joins attached extracting solution, vacuum concentration to there not being the alcohol flavor, vacuum is 0.75MPa, and 60 ℃ of 4 times of being concentrated into original content of temperature are merged into 225ml, fill, sterilization promptly gets the high concentration SHENFU ZHUSHEYE, 25ml/ days.

Embodiment 11The ginseng aconite injection tablet

Radix Ginseng 110g, Radix Aconiti Lateralis Preparata 220g add 70% ethanol extraction 3 times, and each ethanol consumption is 8/6/4 times of medical material amount, extract 2/1/1 hour at every turn, and merge extractive liquid, reclaims ethanol to there not being the alcohol flavor, promptly gets and joins attached extracting solution.The attached extracting solution of above-mentioned ginseng is filtered, and filtrate is by macroporous resin (model is D101), first ethanol elution with 20% following concentration, eluent is abandoned or adopted, reuse adds 4 times of eluting of 60% ethanol, collects eluent, and decompression recycling ethanol is to there not being the alcohol flavor, get purification and join attached extracting solution, lyophilization, Radix Ginseng, Radix Aconiti Lateralis Preparata close and carry thing 13.2g, add mannitol 13.2g, the aseptic condition lower sheeting promptly gets and faces with the preceding ginseng aconite injection tablet of preparation as required.Sheet heavily is the 0.3g/ sheet, 8 slices/day.

Embodiment 12Join attached slow-releasing microcapsule

Radix Ginseng 110g, Radix Aconiti Lateralis Preparata 220g add 70% ethanol extraction 3 times, and each ethanol consumption is 8/6/4 times of medical material amount, extract 2/1/1 hour at every turn, and merge extractive liquid, reclaims ethanol to there not being the alcohol flavor, promptly gets and joins attached extracting solution.The attached extracting solution of above-mentioned ginseng is filtered, filtrate is by macroporous resin (model is D101), and with the ethanol elution of 20% following concentration, eluent is abandoned or adopted earlier, reuse adds 4 times of eluting of 60% ethanol, the collection eluent, decompression recycling ethanol gets purification and joins attached extracting solution to there not being the alcohol flavor, lyophilization, Radix Ginseng, Radix Aconiti Lateralis Preparata close and carry thing 13.2g, add 1 times of amount 10% ethyl cellulose ethanol liquid and 1 times of amount magnesium stearate is mixed, make microcapsule with compressed air by spray congealing.

Embodiment 13Join attached granule

Radix Ginseng 110g, Radix Aconiti Lateralis Preparata 220g add 70% ethanol extraction 3 times, and each ethanol consumption is 8/6/4 times of medical material amount, extract 2/1/1 hour at every turn, and merge extractive liquid, reclaims ethanol to there not being the alcohol flavor, promptly gets and joins attached extracting solution.The attached extracting solution of above-mentioned ginseng is filtered, and filtrate is by macroporous resin (model is D101), first ethanol elution with 20% following concentration, eluent is abandoned or adopted, reuse adds 4 times of eluting of 60% ethanol, collects eluent, and decompression recycling ethanol is to there not being the alcohol flavor, get purification and join attached extracting solution, lyophilization, Radix Ginseng, Radix Aconiti Lateralis Preparata close and carry thing 13.2g, add the dextrin of 2 times of amounts of suitable medicine, ethanol with 60% concentration is made soft material, makes granule.

Embodiment 14Join attached drop pill

Get Radix Ginseng 140g, Radix Aconiti Lateralis Preparata 120g, supersound extraction (ultrasonic power is 500W) 2 times, each 30 minutes, the solvent consumption was 5 times of medical material amount.Merge extractive liquid, reclaims ethanol to there not being the alcohol flavor, promptly gets and joins attached extracting solution.The attached extracting solution of above-mentioned ginseng is filtered, filtrate is by macroporous resin (model is D201), abandon or adopt with 5 times of water gaging eluents earlier, reuse adds 5 times of eluting of 65% ethanol, the collection eluent, decompression recycling ethanol gets purification and joins attached extracting solution to there not being the alcohol flavor, spray drying, Radix Ginseng, Radix Aconiti Lateralis Preparata close and carry thing 7.8g, press medicated powder: substrate 1:1 with substrate PEG6000 fusion after, the adding medicament mixed is even, amount to 19.5g, 75 ℃ of insulations add in the methyl-silicone oil collection drop pill with 50 droplets/minute, absorb liquid coolant with filter paper, make drop pill.Totally 446 balls, the heavy 35mg of every ball, consumption per day 30 balls.One time 15 ball, 2 times/day.

Embodiment 15Join attached capsule

Get Radix Ginseng 150g, Radix Aconiti Lateralis Preparata 100g, supersound extraction (ultrasonic power is 500W) 2 times, each 30 minutes, the solvent consumption was 5 times of medical material amount.Merge extractive liquid, reclaims ethanol to there not being the alcohol flavor, promptly gets and joins attached extracting solution.The attached extracting solution of above-mentioned ginseng is filtered, and filtrate is abandoned or adopted with 5 times of water gaging eluents earlier by macroporous resin (model is D201), and reuse adds 5 times of eluting of 65% ethanol, the collection eluent, and decompression recycling ethanol gets purification and joins attached extracting solution to there not being the alcohol flavor.Spray drying adopts membrane separation technique further refining, and the model of refining used film is FLT-VL, Radix Ginseng, Radix Aconiti Lateralis Preparata close and carry thing 10g, add the dextrin of 15.75g, make 145 of capsules, each 3, day clothes 3 times, 0.25g/ grain.

Embodiment 16Join attached granule

Get Radix Ginseng 130g, Radix Aconiti Lateralis Preparata 110g, supersound extraction (ultrasonic power is 500W) 2 times, each 30 minutes, the solvent consumption was 5 times of medical material amount.Merge extractive liquid, reclaims ethanol to there not being the alcohol flavor, promptly gets and joins attached extracting solution.The attached extracting solution of above-mentioned ginseng is filtered, and filtrate is abandoned or adopted with 5 times of water gaging eluents earlier by macroporous resin (model is D201), and reuse adds 5 times of eluting of 65% ethanol, the collection eluent, and decompression recycling ethanol gets purification and joins attached extracting solution to there not being the alcohol flavor.Spray drying adopts membrane separation technique further refining, and the model made from extra care used film is FLT-Z (II)---FLT-VL, Radix Ginseng, Radix Aconiti Lateralis Preparata close and carry thing 9.6g, add the dextrin of 3 times of amounts of suitable medicine, make soft material with the ethanol of 60% concentration, make granule 38g, each 5g, day clothes 1 time.

Embodiment 17Join attached spray

Get Radix Ginseng 120g, Radix Aconiti Lateralis Preparata 180g, supersound extraction (ultrasonic power is 500W) 2 times, each 30 minutes, the solvent consumption was 5 times of medical material amount.Merge extractive liquid, reclaims ethanol to there not being the alcohol flavor, promptly gets and joins attached extracting solution.The attached extracting solution of above-mentioned ginseng is filtered, filtrate is abandoned or adopted with 5 times of water gaging eluents earlier by macroporous resin (model is D201), and reuse adds 5 times of eluting of 65% ethanol, collect eluent, decompression recycling ethanol gets purification and joins attached extracting solution, spray drying to there not being the alcohol flavor, adopt membrane separation technique further refining, the model of refining used film is FLT-Z (II)---FLT-VL, Radix Ginseng, Radix Aconiti Lateralis Preparata close and carry thing 12g, Radix Ginseng, Radix Aconiti Lateralis Preparata are closed to carry thing fine powder mix homogeneously stand-by; Add the 20% glycerol 150ml that joins, the dissolving moisturizing is to the 300ml mixing, and 50ml or 20ml or 30ml/ bottle are sub-packed in the aerosol pressure bottle, are pressed into dichlorodifluoromethane 2g behind the gland in every bottle, make spray, daily 6-8ml.

Embodiment 18Join attached oral liquid

Get Radix Ginseng 150g, Radix Aconiti Lateralis Preparata 100g, supersound extraction (ultrasonic power is 500W) 2 times, each 30 minutes, the solvent consumption was 5 times of medical material amount.Merge extractive liquid, reclaims ethanol to there not being the alcohol flavor, promptly gets and joins attached extracting solution.The attached extracting solution of above-mentioned ginseng is filtered, filtrate is by macroporous resin (model is D201), abandon or adopt with 5 times of water gaging eluents earlier, reuse adds 5 times of eluting of 65% ethanol, collect eluent, decompression recycling ethanol is to there not being the alcohol flavor, get purification and join attached extracting solution, lyophilization, adopt membrane separation technique further refining, the model of refining used film is FLT-Z (II)---FLT-VG---FLT-VAB---FLT-VAC, Radix Ginseng, Radix Aconiti Lateralis Preparata close and carry thing 17.6g, the stevioside that adds 0.03g adds the injection water again and makes the 166ml oral liquid, 20ml/ days.

Embodiment 19SHENFU ZHUSHEYE

Get Radix Ginseng 110g, Radix Aconiti Lateralis Preparata 100g, supersound extraction (ultrasonic power is 500W) 2 times, each 30 minutes, the solvent consumption was 5 times of medical material amount.Merge extractive liquid, reclaims ethanol to there not being the alcohol flavor, promptly gets and joins attached extracting solution.The attached extracting solution of above-mentioned ginseng is filtered, and filtrate is abandoned or adopted with 5 times of water gaging eluents earlier by macroporous resin (model is D201), reuse adds 5 times of eluting of 65% ethanol, collects eluent, and decompression recycling ethanol is to there not being the alcohol flavor, purification be joined attached extracting solution, spray drying, Radix Ginseng, Radix Aconiti Lateralis Preparata close and carry thing 8.4g, the tween 80 of adding 0.2% is added water for injection to 700ml, regulates pH to 6 with sodium hydroxide, filter, embedding, sterilization promptly gets SHENFU ZHUSHEYE.

Embodiment 20Join attached freeze-dried powder

Get Radix Ginseng 110g, Radix Aconiti Lateralis Preparata 100g, supersound extraction (ultrasonic power is 500W) 2 times, each 30 minutes, the solvent consumption was 5 times of medical material amount.Merge extractive liquid, reclaims ethanol to there not being the alcohol flavor, promptly gets and joins attached extracting solution.The attached extracting solution of above-mentioned ginseng is filtered, filtrate is by macroporous resin (model is D201), abandon or adopt with 5 times of water gaging eluents earlier, reuse adds 5 times of eluting of 65% ethanol, collect eluent, decompression recycling ethanol gets purification and joins attached extracting solution to there not being the alcohol flavor, carries out lyophilization, thickness is no more than 15mm in exsiccant container,-32 ℃ of freezings 13 hours are taken out container and were put into freezer dryer dry 24 hours, are prepared into Radix Ginseng, Radix Aconiti Lateralis Preparata extracts the stimulating food powder, packing under the aseptic condition, promptly get Shenfu freeze driet powder injection, the 3-6 bottle/time, once a day.

Embodiment 21Join attached powder pin

Get Radix Ginseng 110g, Radix Aconiti Lateralis Preparata 100g, supersound extraction (ultrasonic power is 500W) 2 times, each 30 minutes, the solvent consumption was 5 times of medical material amount.Merge extractive liquid, reclaims ethanol to there not being the alcohol flavor, promptly gets and joins attached extracting solution.The attached extracting solution of above-mentioned ginseng is filtered, filtrate is by macroporous resin (model is D201), abandon or adopt with 5 times of water gaging eluents earlier, reuse adds 5 times of eluting of 65% ethanol, collects eluent, and decompression recycling ethanol is to there not being the alcohol flavor, get purification and join attached extracting solution, carry out spray drying, 160 ℃ of drying tower inlet temperature, leaving air temp is 100 ℃ at the bottom of the tower.The relative density of medicine liquid of spraying is 1.15 (65 ℃), and containing amount of solid is 45%, is prepared into the ginseng, prepared aconite root extract powder, and packing under aseptic condition promptly gets and joins attached injectable powder, the 3-6 bottle/time, 1 time/day.

Embodiment 22Join attached infusion solutions

Get Radix Ginseng 110g, Radix Aconiti Lateralis Preparata 100g, supersound extraction (ultrasonic power is 500W) 2 times, each 30 minutes, the solvent consumption was 5 times of medical material amount.Merge extractive liquid, reclaims ethanol to there not being the alcohol flavor, promptly gets and joins attached extracting solution.The attached extracting solution of above-mentioned ginseng is filtered, and filtrate is abandoned or adopted with 5 times of water gaging eluents earlier by macroporous resin (model is D201), and reuse adds 5 times of eluting of 65% ethanol, the collection eluent, and decompression recycling ethanol gets purification and joins attached extracting solution to there not being the alcohol flavor., lyophilization, Radix Ginseng, Radix Aconiti Lateralis Preparata close and carry thing 8.4g, add 0.2% tween 80, add water for injection to 1925ml, regulate pH to 6 with sodium hydroxide, filter, add NaCL and transfer to wait and ooze, embedding, sterilization promptly gets and joins attached infusion solutions, 250ml/ days, 1 time/day.

Embodiment 23Join attached high concentration injection

Get Radix Ginseng 110g, Radix Aconiti Lateralis Preparata 100g, supersound extraction (ultrasonic power is 500W) 2 times, each 30 minutes, the solvent consumption was 5 times of medical material amount.Merge extractive liquid, reclaims ethanol to there not being the alcohol flavor, promptly gets and joins attached extracting solution.The attached extracting solution of above-mentioned ginseng is filtered, and filtrate is abandoned or adopted with 5 times of water gaging eluents earlier by macroporous resin (model is D201), reuse adds 5 times of eluting of 65% ethanol, collects eluent, and decompression recycling ethanol is to there not being the alcohol flavor, get purification and join attached extracting solution, vacuum concentration, vacuum is 0.75MPa, 60 ℃ of 4 times of being concentrated into original content of temperature, merge fill, sterilization, promptly get high concentration SHENFU ZHUSHEYE 175ml, 25ml/ days.

Embodiment 24The ginseng aconite injection tablet

Get Radix Ginseng 110g, Radix Aconiti Lateralis Preparata 100g, supersound extraction (ultrasonic power is 500W) 2 times, each 30 minutes, the solvent consumption was 5 times of medical material amount.Merge extractive liquid, reclaims ethanol to there not being the alcohol flavor, promptly gets and joins attached extracting solution.The attached extracting solution of above-mentioned ginseng is filtered, and filtrate is abandoned or adopted with 5 times of water gaging eluents earlier by macroporous resin (model is D201), reuse adds 5 times of eluting of 65% ethanol, collects eluent, and decompression recycling ethanol is to there not being the alcohol flavor, get purification and join attached extracting solution, lyophilization, Radix Ginseng, Radix Aconiti Lateralis Preparata close and carry thing 8.4g, add mannitol 8.4g, the aseptic condition lower sheeting, promptly get and face with the preceding ginseng aconite injection tablet of preparation as required, sheet heavily is 0.3g, 8 slices/day.

Embodiment 25Join attached slow-releasing microcapsule

Get Radix Ginseng 110g, Radix Aconiti Lateralis Preparata 100g, supersound extraction (ultrasonic power is 500W) 2 times, each 30 minutes, the solvent consumption was 5 times of medical material amount.Merge extractive liquid, reclaims ethanol to there not being the alcohol flavor, promptly gets and joins attached extracting solution.The attached extracting solution of above-mentioned ginseng is filtered, filtrate is by macroporous resin (model is D201), abandon or adopt with 5 times of water gaging eluents earlier, reuse adds 5 times of eluting of 65% ethanol, collects eluent, decompression recycling ethanol is to there not being the alcohol flavor, purification be joined attached extracting solution, lyophilization, Radix Ginseng, Radix Aconiti Lateralis Preparata close and carry thing 8.4g, add 1 times of amount 10% ethyl cellulose ethanol liquid and the mixing of 1 times of amount magnesium stearate, make microcapsule by spray congealing with compressed air.

Embodiment 26The agent of ginseng Radix Aconiti Lateralis Preparata

Get Radix Ginseng 110g, Radix Aconiti Lateralis Preparata 100g, supersound extraction (ultrasonic power is 500W) 2 times, each 30 minutes, the solvent consumption was 5 times of medical material amount.Merge extractive liquid, reclaims ethanol to there not being the alcohol flavor, promptly gets and joins attached extracting solution.The attached extracting solution of above-mentioned ginseng is filtered, and filtrate is abandoned or adopted with 5 times of water gaging eluents earlier by macroporous resin (model is D201), reuse adds 5 times of eluting of 65% ethanol, collects eluent, and decompression recycling ethanol is to there not being the alcohol flavor, get purification and join attached extracting solution, lyophilization gets Radix Ginseng, Radix Aconiti Lateralis Preparata extract 12g, adds an amount of magnesium stearate, mix, tabletting to 120 slice, the bag film-coat must be joined the Radix Aconiti Lateralis Preparata agent.

Embodiment 27SHENFU ZHUSHEYE

Get Radix Ginseng 100g and add 80% ethanol, supersound extraction (ultrasonic power is 400W) 4 times was respectively 30,30,25,25 minutes, and the solvent consumption is 6 times of medical material amount, and merge extractive liquid, reclaims ethanol to there not being the ethanol flavor, gets the Radix Ginseng extractive solution.Get Radix Aconiti Lateralis Preparata 200g and add 80% ethanol, supersound extraction (ultrasonic power is 400W) 4 times was respectively 30,30,25,25 minutes, and the solvent consumption is 6 times of medical material amount, and merge extractive liquid, gets the Radix Aconiti Lateralis Preparata extracting solution.Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is respectively by macroporous resin (model is D101), earlier with 4 times of water gaging eluting, eluent is abandoned or adopted, reuse adds 5 times of eluting of 65% ethanol, the collection eluent, decompression recycling ethanol gets purification Radix Ginseng extractive solution, purification Radix Aconiti Lateralis Preparata extracting solution to there not being the alcohol flavor.Spray drying, adopt membrane separation technique further refining, the model made from extra care used film is FLT-Z (II)---FLT-VG---FLT-VAB---FLT-VAC, gets Radix Ginseng extract 3g, Radix Aconiti Lateralis Preparata extract 6g, Radix Ginseng extract, Radix Aconiti Lateralis Preparata extract powder mixes is even, the tween 80 of adding 0.2% is added water for injection to 1000ml, regulates pH to 6 with sodium hydroxide, filter, embedding, sterilization promptly gets SHENFU ZHUSHEYE.

Embodiment 28Join attached freeze-dried powder

Get Radix Ginseng 80g and add 60% ethanol, supersound extraction (ultrasonic power 500W) 3 times was respectively 30,30,25 minutes, and the solvent consumption is 4 times of medical material amount, and merge extractive liquid, gets the Radix Ginseng extractive solution.Get the sour water (adopting concentrated hydrochloric acid to regulate) that Radix Aconiti Lateralis Preparata 160g adds pH2～3, supersound extraction (ultrasonic power is 500W) 3 times was respectively 30,30,25 minutes, the solvent consumption is 4～6 times of medical material amount, merge extractive liquid,, and being concentrated into 60 ℃ of relative densities is 1.25, add ethanol and make concentration of alcohol reach 70%, cold preservation 24 hours filters, reclaim ethanol to there not being the alcohol flavor, add ethanol and make concentration of alcohol reach 80%, cold preservation 24 hours filters to clarification, reclaim ethanol to there not being the ethanol flavor, get the Radix Aconiti Lateralis Preparata extracting solution; Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, (model is D601 to filtrate by macroporous resin respectively,), earlier with 5 times of water gaging eluting, eluent is abandoned or adopted, and reuse adds 10 times of eluting of 60% ethanol, collects eluent, decompression recycling ethanol gets purification Radix Ginseng extractive solution, purification Radix Aconiti Lateralis Preparata extracting solution to there not being the alcohol flavor.Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution mix homogeneously are carried out lyophilization, thickness is no more than 15mm in exsiccant container,-32 ℃ of freezings 13 hours, take out container and put into freezer dryer dry 24 hours, be prepared into Radix Ginseng extract powder 4.5g, Radix Aconiti Lateralis Preparata extract powder 6g, packing under the aseptic condition promptly gets Shenfu freeze driet powder injection.

Embodiment 29Join attached powder pin

Get Radix Ginseng 70g and add 70% ethanol, supersound extraction (ultrasonic power is 500W) 3 times was respectively 30,25,25 minutes, and the solvent consumption is 5 times of medical material amount, and merge extractive liquid, gets the Radix Ginseng extractive solution.Get Radix Aconiti Lateralis Preparata 140g and add 70% ethanol, reflux, extract, 4 times was respectively 2,2,1,1 hours, and the solvent consumption is 5 times of medical material amount, and merge extractive liquid, gets the Radix Aconiti Lateralis Preparata extracting solution.Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is respectively by macroporous resin (model is D601), measure 12% ethanol elution with 4 times earlier, eluent is abandoned or adopted, reuse adds 6 times of eluting of 75% ethanol, the collection eluent, decompression recycling ethanol gets purification Radix Ginseng extractive solution, purification Radix Aconiti Lateralis Preparata extracting solution to there not being the alcohol flavor.Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution mix homogeneously are carried out spray drying, 160 ℃ of drying tower inlet temperature, leaving air temp is 100 ℃ at the bottom of the tower.The relative density of medicine liquid of spraying is 1.15 (65 ℃), and containing amount of solid is 45%, is prepared into Radix Ginseng extract powder 3g, Radix Aconiti Lateralis Preparata extract powder 4g, and packing under aseptic condition promptly gets and joins attached injectable powder, the 0.2g/ bottle.

Embodiment 30Join attached infusion solutions

Get Radix Ginseng 100g and add 75% ethanol, supersound extraction (ultrasonic power is 600W) 3 times was respectively 30,25,25 minutes, and the solvent consumption is 6 times of medical material amount, and merge extractive liquid, gets the Radix Ginseng extractive solution.Get the sour water (adopting concentrated hydrochloric acid to regulate) that Radix Aconiti Lateralis Preparata 180g adds pH2, decoct and extract 3 times, be respectively 2,1,1 hours, the solvent consumption is 4.5 times of medical material amount, merge extractive liquid,, and being concentrated into 50 ℃ of relative densities is 1.1, add ethanol and make concentration of alcohol reach 70%, cold preservation 26 hours filters, reclaim ethanol to there not being the alcohol flavor, add ethanol and make concentration of alcohol reach 80%, cold preservation 30 hours filters to clarification, reclaim ethanol to there not being the ethanol flavor, get the Radix Aconiti Lateralis Preparata extracting solution; Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is respectively by macroporous resin (model is HP-20), earlier with 5 times of water gaging eluting, eluent is abandoned or adopted, reuse adds 5 times of eluting of 70% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get the extraction of purification Radix Ginseng, purification Radix Aconiti Lateralis Preparata extracting solution.With Radix Ginseng extractive solution, the lyophilization of Radix Aconiti Lateralis Preparata extracting solution mix homogeneously, adopt membrane separation technique further refining, the model made from extra care used film is FLT-Z (II)---FLT-VL, get Radix Ginseng extract 4g, Radix Aconiti Lateralis Preparata extract 10g, the tween 80 of adding 0.2% is added water for injection to 2500ml, regulates pH to 6 with sodium hydroxide, filter, add NaCL accent etc. and ooze, embedding, sterilization, promptly get and join attached infusion solutions, 250ml/ days.

Embodiment 31Join attached high concentration injection

Get Radix Ginseng 160g and add water, supersound extraction (ultrasonic power is 700W) 3 times was respectively 35,30,20 minutes, and the solvent consumption is 5 times of medical material amount.Merge extractive liquid,, being concentrated into 60 ℃ of relative densities is 1.2, add ethanol and make concentration of alcohol reach 75%, cold preservation 24 hours, filtration is reclaimed ethanol and is not distinguished the flavor of to there being alcohol, adds ethanol and makes concentration of alcohol reach 85%, and cold preservation 28 hours filters to clarification, gets the Radix Ginseng extractive solution.Get Radix Aconiti Lateralis Preparata 180g and add 80% ethanol, supersound extraction (ultrasonic power is 800W) 3 times was respectively 25,20,20 minutes, and the solvent consumption is 6 times of medical material amount, and merge extractive liquid, gets the Radix Aconiti Lateralis Preparata extracting solution.Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is respectively by macroporous resin (model is D101), first ethanol elution with 4～6 times of water gagings or 20% following concentration, eluent is abandoned or adopted, reuse adds 2 times of eluting of 85% ethanol, collection eluent, decompression recycling ethanol get purification Radix Ginseng extractive solution (quite Radix Ginseng extract powder 10g), purification Radix Aconiti Lateralis Preparata extracting solution (quite Radix Aconiti Lateralis Preparata extract powder 12g) to there not being the alcohol flavor.Vacuum concentration, vacuum are 0.75MPa, and 60 ℃ of 4 times of being concentrated into original content of temperature merge, fill, and sterilization promptly got the high concentration SHENFU ZHUSHEYE 225m1.25ml/ days.

Embodiment 32The ginseng aconite injection tablet

Get Radix Ginseng 60g and add water, supersound extraction (ultrasonic power is 800W) 3 times was respectively 30,30,20 minutes, and the solvent consumption is 5 times of medical material amount.Merge extractive liquid,, being concentrated into 60 ℃ of relative densities is 1.3, add ethanol and make concentration of alcohol reach 75%, cold preservation 20 hours, filtration is reclaimed ethanol and is not distinguished the flavor of to there being alcohol, adds ethanol and makes concentration of alcohol reach 85%, and cold preservation 30 hours filters to clarification, gets the Radix Ginseng extractive solution.Get Radix Aconiti Lateralis Preparata 120g and add water, supersound extraction (ultrasonic power is 800W) 3 times, be respectively 30,30,20 minutes, the solvent consumption is 6 times of medical material amount, merge extractive liquid,, and being concentrated into 50 ℃ of relative densities is 1.1, adding ethanol makes concentration of alcohol reach 70%, cold preservation 30 hours filters, and reclaims ethanol to there not being the alcohol flavor, adding ethanol makes concentration of alcohol reach 80%, cold preservation 30 hours filters to clarification, reclaims ethanol to there not being the ethanol flavor, get the Radix Aconiti Lateralis Preparata extracting solution with above-mentioned Radix Ginseng extractive solution, the Radix Aconiti Lateralis Preparata extracting solution filters respectively, filtrate is respectively by macroporous resin (model is D601), and with the ethanol elution of 4 times of amounts 18%, eluent is abandoned or adopted earlier, reuse adds 6 times of eluting of 70% ethanol, the collection eluent, decompression recycling ethanol gets the purification Radix Ginseng extractive solution to there not being the alcohol flavor, purification Radix Aconiti Lateralis Preparata extracting solution.Spray drying, adopt membrane separation technique further refining, the model of refining used film is FLT-VL, Radix Ginseng extract 3g, Radix Aconiti Lateralis Preparata extract 6g, Radix Ginseng extract, Radix Aconiti Lateralis Preparata extract powder mixes is even, add lactose 9g, the aseptic condition lower sheeting promptly gets 48 in the tablet of ginseng aconite injection that faces with preceding preparation as required, and sheet heavily is 0.3g, one time 8,1 time/day.

Embodiment 33Join attached slow-releasing microcapsule

Get Radix Ginseng 55g and add water, supersound extraction (ultrasonic power is 700W) 3 times was respectively 30,30,20 minutes, and the solvent consumption is 6 times of medical material amount.Merge extractive liquid,, being concentrated into 55 ℃ of relative densities is 1.15, add ethanol and make concentration of alcohol reach 75%, cold preservation 18 hours, filtration is reclaimed ethanol and is not distinguished the flavor of to there being alcohol, adds ethanol and makes concentration of alcohol reach 85%, and cold preservation 30 hours filters to clarification, gets the Radix Ginseng extractive solution.Get Radix Aconiti Lateralis Preparata 110g and add 80% ethanol, reflux, extract, 3 times was respectively 2,1,1 hours, and the solvent consumption is 6 times of medical material amount, and merge extractive liquid, gets the Radix Aconiti Lateralis Preparata extracting solution.With above-mentioned Radix Ginseng extractive solution, the Radix Aconiti Lateralis Preparata extracting solution filters respectively, filtrate is respectively by macroporous resin (model is D201), measure 10% ethanol elution with 5 times earlier, eluent is abandoned or adopted, reuse adds 3 times of eluting of 85% ethanol, collect eluent, decompression recycling ethanol is to there not being the alcohol flavor, get the purification Radix Ginseng extractive solution, purification Radix Aconiti Lateralis Preparata extracting solution, spray drying, adopt membrane separation technique further refining, the model made from extra care used film is FLT-Z (II)---FLT-VL, gets Radix Ginseng extract 2.75g, Radix Aconiti Lateralis Preparata extract 5.5g is with Radix Ginseng extract, the Radix Aconiti Lateralis Preparata extract powder mixes is even, add 1 times of amount 10% ethyl cellulose ethanol liquid and the mixing of 1 times of amount magnesium stearate, make microcapsule by spray congealing with compressed air.

Embodiment 34Join attached capsule

Get Radix Ginseng 65g and add water, supersound extraction (ultrasonic power is 300W) 4 times was respectively 40,40,30,20 minutes, and the solvent consumption is 6 times of medical material amount.Merge extractive liquid,, being concentrated into 60 ℃ of relative densities is 1.2, add ethanol and make concentration of alcohol reach 75%, cold preservation 20 hours, filtration is reclaimed ethanol and is not distinguished the flavor of to there being alcohol, adds ethanol and makes concentration of alcohol reach 85%, and cold preservation 30 hours filters to clarification, gets the Radix Ginseng extractive solution.Get the sour water (adopting concentrated hydrochloric acid to regulate) that Radix Aconiti Lateralis Preparata 130g adds pH3, decoct and extract 3 times, be respectively 2,2,1 hour, the solvent consumption is 4 times of medical material amount, merge extractive liquid,, being concentrated into 55 ℃ of relative densities is 1.3, adds ethanol and makes concentration of alcohol reach 70%, cold preservation 24 hours, filter, reclaim ethanol, add ethanol and make concentration of alcohol reach 80% to there not being the alcohol flavor, cold preservation 32 hours, filtration is reclaimed ethanol to there being the ethanol flavor to clarification, gets the Radix Aconiti Lateralis Preparata extracting solution with above-mentioned Radix Ginseng extractive solution, the Radix Aconiti Lateralis Preparata extracting solution filters respectively, filtrate is respectively by macroporous resin (model is D601), with 6 times of water gaging eluting, eluent is abandoned or adopted earlier, and reuse adds 2 times of eluting of 85% ethanol, collect eluent, decompression recycling ethanol gets the purification Radix Ginseng extractive solution to there not being the alcohol flavor, purification Radix Aconiti Lateralis Preparata extracting solution, spray drying get Radix Ginseng extract powder 3.9g, Radix Aconiti Lateralis Preparata extract powder 7.8g, add the dextrin of 15.75g, make capsule.

Embodiment 35Join attached freeze-dried powder

Get Radix Ginseng 100g water extraction 3 times, each 2 hours, the solvent consumption was 4 times of medical material amount.Merge extractive liquid,, being concentrated into 60 ℃ of relative densities is 1.3, add ethanol and make concentration of alcohol reach 75%, cold preservation 19 hours, filtration adds ethanol and makes concentration of alcohol reach 85%, and cold preservation 28 hours filters to clarification, gets the Radix Ginseng extractive solution.Get Radix Aconiti Lateralis Preparata 100g and add 80% ethanol, supersound extraction (ultrasonic power is 700W) 3 times was respectively 30,30,20 minutes, and the solvent consumption is 5.5 times of medical material amount, and merge extractive liquid, gets the Radix Aconiti Lateralis Preparata extracting solution.Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is respectively by macroporous resin (model is D601), earlier with 6 times of water gaging eluting, eluent is abandoned or adopted, reuse adds 8 times of eluting of 65% ethanol, the collection eluent, decompression recycling ethanol gets purification Radix Ginseng extractive solution, purification Radix Aconiti Lateralis Preparata extracting solution to there not being the alcohol flavor.Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution mix homogeneously are carried out lyophilization, thickness is no more than 15mm in exsiccant container,-32 ℃ of freezings 13 hours, take out container and put into freezer dryer dry 24 hours, be prepared into Radix Ginseng extract powder 5g, Radix Aconiti Lateralis Preparata extract powder 4g, packing under the aseptic condition promptly gets Shenfu freeze driet powder injection.

Embodiment 36Join attached powder pin

Get Radix Ginseng 70g water extraction 2 times, each 2 hours, the solvent consumption was 5 times of medical material amount.Merge extractive liquid,, being concentrated into 55 ℃ of relative densities is 1.25, add ethanol and make concentration of alcohol reach 75%, cold preservation 17 hours, filtration adds ethanol and makes concentration of alcohol reach 85%, and cold preservation 34 hours filters to clarification, gets the Radix Ginseng extractive solution.Get Radix Aconiti Lateralis Preparata 100g and add water, supersound extraction (ultrasonic power is 750W) 3 times, each 30,25,20 minutes, the solvent consumption is 6 times of medical material amount, merge extractive liquid,, and being concentrated into 55 ℃ of relative densities is 1.15, add ethanol and make concentration of alcohol reach 70%, cold preservation 36 hours filters, reclaim ethanol to there not being the alcohol flavor, add ethanol and make concentration of alcohol reach 80%, cold preservation 30 hours filters to clarification, reclaim ethanol to there not being the ethanol flavor, get the Radix Aconiti Lateralis Preparata extracting solution; Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is respectively by macroporous resin (model is D601), measure 12% ethanol elution with 4 times earlier, eluent is abandoned or adopted, reuse adds 4 times of eluting of 75% ethanol, the collection eluent, decompression recycling ethanol gets purification Radix Ginseng extractive solution, purification Radix Aconiti Lateralis Preparata extracting solution to there not being the alcohol flavor.Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution mix homogeneously are carried out spray drying, 160 ℃ of drying tower inlet temperature, leaving air temp is 100 ℃ at the bottom of the tower.The relative density of medicine liquid of spraying is 1.15 (65 ℃), and containing amount of solid is 45%, is prepared into Radix Ginseng extract powder 3.5g, Radix Aconiti Lateralis Preparata extract powder 5g, and packing under aseptic condition promptly gets and joins attached injectable powder.

Embodiment 37Join attached infusion solutions

Get Radix Ginseng 80g water extraction 3 times, be respectively 2,2,1 hours, the solvent consumption is 6 times of medical material amount.Merge extractive liquid,, being concentrated into 60 ℃ of relative densities is 1.1, add ethanol and make concentration of alcohol reach 75%, cold preservation 28 hours, filtration adds ethanol and makes concentration of alcohol reach 85%, and cold preservation 30 hours filters to clarification, gets the Radix Ginseng extractive solution.Get Radix Aconiti Lateralis Preparata 160g and add 75% ethanol, reflux, extract, 3 times, each 1.5 hours, the solvent consumption was 5 times of medical material amount, merge extractive liquid, gets the Radix Aconiti Lateralis Preparata extracting solution.Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is respectively by macroporous resin (model is D101), first ethanol elution with 4～6 times of water gagings or 20% following concentration, eluent is abandoned or adopted, reuse adds 1～10 times of eluting of 60～90% ethanol, the collection eluent, decompression recycling ethanol gets purification Radix Ginseng extractive solution, purification Radix Aconiti Lateralis Preparata extracting solution to there not being the alcohol flavor.With Radix Ginseng extractive solution, the lyophilization of Radix Aconiti Lateralis Preparata extracting solution mix homogeneously, get Radix Ginseng extract powder 4g, Radix Aconiti Lateralis Preparata extract 8g, add 0.2% tween 80, add water for injection to 2000ml, regulate pH to 6 with sodium hydroxide, filter, add NaCL accent etc. and ooze, embedding, sterilization promptly gets and joins attached infusion solutions.

Embodiment 38Join attached high concentration injection

Get Radix Ginseng 150g water extraction 3 times, be respectively 2,2,1 hours, the solvent consumption is 6 times of medical material amount.Merge extractive liquid,, being concentrated into 50 ℃ of relative densities is 1.15, add ethanol and make concentration of alcohol reach 75%, cold preservation 35 hours, filtration adds ethanol and makes concentration of alcohol reach 85%, and cold preservation 26 hours filters to clarification, gets the Radix Ginseng extractive solution.Get the sour water (adopting concentrated hydrochloric acid to regulate) that Radix Aconiti Lateralis Preparata 100g adds pH=3, decoct and extract 3 times, be respectively 2,1,1 hours, the solvent consumption is 6 times of medical material amount, merge extractive liquid,, and being concentrated into 60 ℃ of relative densities is 1.3, add ethanol and make concentration of alcohol reach 70%, cold preservation 30 hours filters, reclaim ethanol to there not being the alcohol flavor, add ethanol and make concentration of alcohol reach 80%, cold preservation 32 hours filters to clarification, reclaim ethanol to there not being the ethanol flavor, get the Radix Aconiti Lateralis Preparata extracting solution; Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is respectively by macroporous resin (model is HP-20), earlier with 5 times of water gaging eluting, eluent is abandoned or adopted, reuse adds 4 times of eluting of 70% ethanol, collection eluent, decompression recycling ethanol get purification Radix Ginseng extractive solution (quite Radix Ginseng is carried thing powder 10g), purification Radix Aconiti Lateralis Preparata extracting solution (quite Radix Aconiti Lateralis Preparata is carried thing powder 2.5g) to there not being the alcohol flavor.Vacuum concentration, vacuum are 0.75MPa, and 60 ℃ of 4 times of being concentrated into original content of temperature merge, fill, and sterilization promptly gets the high concentration SHENFU ZHUSHEYE.

Embodiment 39The ginseng aconite injection tablet

Get Radix Ginseng 60g and add 60% ethanol, reflux, extract, 4 times, each 1.5 hours, the solvent consumption was 6 times of medical material amount, merge extractive liquid, gets the Radix Ginseng extractive solution.Get Radix Aconiti Lateralis Preparata 250g and add 75% ethanol, supersound extraction (ultrasonic power is 600W) 3 times was respectively 40,30,30 minutes, and the solvent consumption is 6 times of medical material amount, and merge extractive liquid, gets the Radix Aconiti Lateralis Preparata extracting solution.Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is respectively by macroporous resin (model is D201), earlier with 6 times of water gaging eluting, eluent is abandoned or adopted, reuse adds 5 times of eluting of 85% ethanol, the collection eluent, decompression recycling ethanol gets purification Radix Ginseng extractive solution, purification Radix Aconiti Lateralis Preparata extracting solution to there not being the alcohol flavor.Spray drying, Radix Ginseng extract 2.4g, Radix Aconiti Lateralis Preparata extract 17g, Radix Ginseng extract, Radix Aconiti Lateralis Preparata extract powder mixes is even, add mannitol 8.4g, the aseptic condition lower sheeting promptly gets and faces the ginseng aconite injection tablet of preparing as required with preceding, sheet heavily is 0.3g.

Embodiment 40Join attached slow-releasing microcapsule

Get Radix Ginseng 55g and add 80% ethanol, reflux, extract, 3 times was respectively 2,1,1 hours, and the solvent consumption is 4 times of medical material amount, and merge extractive liquid, gets the Radix Ginseng extractive solution.Get Radix Aconiti Lateralis Preparata 120g and add water, supersound extraction (ultrasonic power is 800W) 3 times was respectively 35,30,20 minutes, the solvent consumption is 6 times of medical material amount, merge extractive liquid,, and being concentrated into 60 ℃ of relative densities is 1.3, add ethanol and make concentration of alcohol reach 70%, cold preservation 24 hours filters, reclaim ethanol to there not being the alcohol flavor, add ethanol and make concentration of alcohol reach 80%, cold preservation 26 hours filters to clarification, reclaim ethanol to there not being the ethanol flavor, get the Radix Aconiti Lateralis Preparata extracting solution; Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is respectively by macroporous resin (model is D601), measure 15% ethanol elution with 6 times earlier, eluent is abandoned or adopted, reuse adds 9 times of eluting of 65% ethanol, the collection eluent, decompression recycling ethanol gets purification Radix Ginseng extractive solution, purification Radix Aconiti Lateralis Preparata extracting solution to there not being the alcohol flavor.Spray drying, Radix Ginseng extract 2.75g, Radix Aconiti Lateralis Preparata extract 6g, Radix Ginseng extract, Radix Aconiti Lateralis Preparata extract powder mixes is even, be heated to 50 as suspension or O/W type emulsion among the 40g/L gelatin solution 100ml, add acetic acid and transfer to PH=6, add flocculating agent Na ₂SO ₄Solution must condense capsule, adds diluent and gets the sedimentation capsule, is chilled to below 15 ℃ to add formalin, transfers to PH8～9 with NaOH solution and must solidify capsule, washes with water to the formaldehydeless microcapsule that gets.

Embodiment 41Join attached rapid release drop pill

Get Radix Ginseng 65g and add 65% ethanol, reflux, extract, 4 times, each 2 hours, the solvent consumption was 3 times of medical material amount, merge extractive liquid, gets the Radix Ginseng extractive solution.Get the sour water (adopting concentrated hydrochloric acid to regulate) that Radix Aconiti Lateralis Preparata 150g adds pH=2.5, decoct and extract each 1.5 hours 2 times, the solvent consumption is 5 times of medical material amount, merge extractive liquid,, and being concentrated into 60 ℃ of relative densities is 1.25, add ethanol and make concentration of alcohol reach 70%, cold preservation 26 hours filters, reclaim ethanol to there not being the alcohol flavor, add ethanol and make concentration of alcohol reach 80%, cold preservation 34 hours filters to clarification, reclaim ethanol to there not being the ethanol flavor, get the Radix Aconiti Lateralis Preparata extracting solution; Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is respectively by macroporous resin (model is HP-20), earlier with 6 times of ethanol elutions of measuring 18% following concentration, eluent is abandoned or adopted, reuse adds 7 times of eluting of 75% ethanol, collects eluent, and decompression recycling ethanol is to there not being the alcohol flavor, be concentrated into proportion 1.06, get purification Radix Ginseng extractive solution, purification Radix Aconiti Lateralis Preparata extracting solution.Spray drying, get Radix Ginseng extract 3.25g, Radix Aconiti Lateralis Preparata extract 7.5g, Radix Ginseng extract, Radix Aconiti Lateralis Preparata extract powder mixes is even, join in the substrate that 32.25g is made into the ratio of 4:1 by Polyethylene Glycol (PEC) 6000 and lecithin (PC), it is liquid to be heated to 90 ℃ of one-tenth, splash into 50 droplets/minute speed and to be cooled to ball in the liquid Paraffin, the 40mg/ ball is made 1500 balls.

Embodiment 42Capsule

Radix Ginseng alcohol extraction supersound extraction is crossed the refining thing 50g of post, and Radix Aconiti Lateralis Preparata water is put forward supersound extraction and crossed the refining thing 20g of post, makes 420 of capsules, and each 3,3 times/day.

Embodiment 43Tablet

The refining thing 12g of post is crossed in the Radix Ginseng alcohol extraction, and Radix Aconiti Lateralis Preparata water is put forward supersound extraction and crossed the refining thing 16g of post, makes 280 in tablet, and each 3,3 times/day.

Embodiment 44Granule

Radix Ginseng water is put forward supersound extraction and is crossed the refining thing 14g of post, and the refining thing 5g of post is crossed in the Radix Aconiti Lateralis Preparata alcohol extraction, makes granule 500 grams, and 10g/ time, 2 times/day.

Embodiment 45Drop pill

Radix Ginseng alcohol extraction supersound extraction is crossed the refining thing 4g of post, and Radix Aconiti Lateralis Preparata alcohol extraction supersound extraction is crossed the refining thing 8g of post, and often regulation becomes drop pill 720 balls, the heavy 35mg of every ball, consumption per day 48 balls.One time 24 ball, 2 times/day.

Claims (11)

1. join the preparation method of attached high concentration injection formulation, it is characterized in that this high concentration injection formulation made by following method: Radix Ginseng extract 1.5-18 weight portions, Radix Aconiti Lateralis Preparata extract 1.5-18 weight portions, with purification Radix Ginseng extractive solution and purification Radix Aconiti Lateralis Preparata extracting solution difference vacuum concentration, vacuum is 0.08-0.9MPa, 40-80 ℃ of temperature, be concentrated into 1-5 times of original content, be 1-5 times of SHENFU ZHUSHEYE concentration commonly used, be the crude drug amount that is equivalent to that contains in every ml high concentration injection: Radix Ginseng 0.1-0.5g, Radix Aconiti Lateralis Preparata 0.2-1g merge concentrated solution; Transfer PH=5-7 fill, sterilization promptly gets the high concentration SHENFU ZHUSHEYE.

2. the preparation method of the attached high concentration injection formulation of ginseng as claimed in claim 1 is characterized in that this high concentration injection formulation raw material is: Radix Ginseng extract 4-12 weight portions, Radix Aconiti Lateralis Preparata extract 4-12 weight portions.

3. the preparation method of the attached high concentration injection formulation of ginseng as claimed in claim 1 is characterized in that this high concentration injection formulation raw material is: Radix Ginseng extract 4.5-7.5 weight portions, Radix Aconiti Lateralis Preparata extract 9-15 weight portions.

4. the preparation method of the attached high concentration injection formulation of ginseng as claimed in claim 1, it is characterized in that this joins attached high concentration injection formulation and made by following method: Radix Ginseng extract 4 weight portions, Radix Aconiti Lateralis Preparata extract 8 weight portions, vacuum concentration, vacuum are 0.75MPa, 60 ℃ of 4 times of being concentrated into original content of temperature, merge, fill, the high concentration injection formulation is made in sterilization.

5. as the preparation method of the attached high concentration injection formulation of each described ginseng of claim 1-4, it is characterized in that this raw material Radix Ginseng extract of joining attached high concentration injection formulation is that the alcohol extraction supersound extraction is crossed the refining thing of post, water and put forward supersound extraction and cross the refining thing of post, alcohol extraction and cross the refining thing of post or water and carried in the refining thing of post any one; Radix Aconiti Lateralis Preparata extract is that the alcohol extraction supersound extraction is crossed the refining thing of post, water and put forward supersound extraction and cross the refining thing of post, alcohol extraction and cross the refining thing of post or water and carried in the refining thing of post any one.

6. the preparation method of the attached high concentration injection formulation of ginseng as claimed in claim 5 is characterized in that the preparation method of raw material Radix Ginseng extract and Radix Aconiti Lateralis Preparata extract is prepared by following any one method:

A, get the people and participate in 60-80% ethanol, supersound extraction 3-4 times, ultrasonic power is 250-800W, each 20-45 minutes, the solvent consumption was 4-6 times of medical material amount, merge extractive liquid, reclaims ethanol to there being the ethanol flavor, the Radix Ginseng extractive solution; Get Radix Aconiti Lateralis Preparata and add 60-80% ethanol, supersound extraction 3-4 times, ultrasonic power is 250-800W, and each 20-45 minutes, the solvent consumption was 4-6 times of medical material amount, and merge extractive liquid, gets the Radix Aconiti Lateralis Preparata extracting solution; Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is passed through macroporous resin respectively, first ethanol elution with 4-6 times of water gagings or 20% following concentration, eluent is abandoned or adopted, reuse adds 1-10 times of eluting of 60-90% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution respectively;

B, getting the people and participate in 60-80% ethanol, is the supersound extraction 3-4 times of 250-800W with ultrasonic power, and each 20-45 minutes, the solvent consumption was 4-6 times of medical material amount, merge extractive liquid,, the Radix Ginseng extractive solution; Getting Radix Aconiti Lateralis Preparata adds water or adds that to be adjusted to pH with concentrated hydrochloric acid be 2-3 sour water, with ultrasonic power is the supersound extraction 3-4 times of 250-800W, each 20-45 minutes, the solvent consumption is 4-6 times of medical material amount, merge extractive liquid,, being concentrated into 50-60 ℃ of relative densities is 1.1-1.3, add ethanol and make concentration of alcohol reach 70%, cold preservation 24-36 hours filters, reclaim ethanol to there not being the alcohol flavor, add ethanol and make concentration of alcohol reach 80%, cold preservation 24-36 hours filters to clarification, reclaim ethanol to there not being the ethanol flavor, get the Radix Aconiti Lateralis Preparata extracting solution; Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is passed through macroporous resin respectively, first ethanol elution with 4-6 times of water gagings or 20% following concentration, eluent is abandoned or adopted, reuse adds 1-10 times of eluting of 60-90% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the attached extract that gives of getting of purification Radix Aconiti Lateralis Preparata extracting solution;

C, getting the people and participate in 60-80% ethanol, is the supersound extraction 3-4 times of 250-800W with ultrasonic power, and each 20-45 minutes, the solvent consumption was 4-6 times of medical material amount, merge extractive liquid,, the Radix Ginseng extractive solution; Get Radix Aconiti Lateralis Preparata and add 60-80% ethanol, reflux, extract, 3-4 times, each 1-2 hours, the solvent consumption was 4-6 times of medical material amount, merge extractive liquid, gets the Radix Aconiti Lateralis Preparata extracting solution; Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is passed through macroporous resin respectively, first ethanol elution with 4-6 times of water gagings or 20% following concentration, eluent is abandoned or adopted, reuse adds 1-10 times of eluting of 60-90% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution;

D, getting the people and participate in 60-80% ethanol, is the supersound extraction 3-4 times of 250-800W with ultrasonic power, and each 20-45 minutes, the solvent consumption was 4-6 times of medical material amount, merge extractive liquid,, the Radix Ginseng extractive solution; Get Radix Aconiti Lateralis Preparata and add water or concentrated hydrochloric acid to regulate pH be 2-3 sour water, decoct and extract each 1-2 hours 3-4 times, the solvent consumption is 4-6 times of medical material amount, merge extractive liquid,, and being concentrated into 50-60 ℃ of relative densities is 1.1-1.3, add ethanol and make concentration of alcohol reach 70%, cold preservation 24-36 hours filters, reclaim ethanol to there not being the alcohol flavor, add ethanol and make concentration of alcohol reach 80%, cold preservation 24-36 hours filters to clarification, reclaim ethanol to there not being the ethanol flavor, get the Radix Aconiti Lateralis Preparata extracting solution; Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is passed through macroporous resin respectively, first ethanol elution with 4-6 times of water gagings or 20% following concentration, eluent is abandoned or adopted, reuse adds 1-10 times of eluting of 60-90% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution;

E, getting the people and participate in water, is the supersound extraction 3-4 times of 250-800W with ultrasonic power, and each 20-45 minutes, the solvent consumption was 4-6 times of medical material amount; Merge extractive liquid,, being concentrated into 50-60 ℃ of relative densities is 1.1-1.3, adds ethanol and makes concentration of alcohol reach 75%, cold preservation 10-48 hours, filter, reclaim ethanol, add ethanol and make concentration of alcohol reach 85% to there not being the alcohol flavor, cold preservation 24-36 hours filters to clarification, gets the Radix Ginseng extractive solution; Getting Radix Aconiti Lateralis Preparata and add 60-80% ethanol, is the supersound extraction 3-4 times of 250-800W with ultrasonic power, and each 20-45 minutes, the solvent consumption was 4-6 times of medical material amount, merge extractive liquid,, the Radix Aconiti Lateralis Preparata extracting solution; Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is passed through macroporous resin respectively, first ethanol elution with 4-6 times of water gagings or 20% following concentration, eluent is abandoned or adopted, reuse adds 1-10 times of eluting of 60-90% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution;

F, getting the people and participate in water, is the supersound extraction 3-4 times of 250-800W with ultrasonic power, and each 20-45 minutes, the solvent consumption was 4-6 times of medical material amount; Merge extractive liquid,, being concentrated into 50-60 ℃ of relative densities is 1.1-1.3, adds ethanol and makes concentration of alcohol reach 75%, cold preservation 10-48 hours, filter, reclaim ethanol, add ethanol and make concentration of alcohol reach 85% to there not being the alcohol flavor, cold preservation 24-36 hours filters to clarification, gets the Radix Ginseng extractive solution; Getting Radix Aconiti Lateralis Preparata adds water or concentrated hydrochloric acid to regulate pH is 2-3 sour water, with ultrasonic power is the supersound extraction 3-4 times of 250-800W, each 20-45 minutes, the solvent consumption is 4-6 times of medical material amount, merge extractive liquid,, being concentrated into 50-60 ℃ of relative densities is 1.1-1.3, adding ethanol makes concentration of alcohol reach 70%, cold preservation 24-36 hours filters, and reclaims ethanol to there not being the alcohol flavor, adding ethanol makes concentration of alcohol reach 80%, cold preservation 24-36 hours filters to clarification, reclaims ethanol to there not being the ethanol flavor, get the Radix Aconiti Lateralis Preparata extracting solution with above-mentioned Radix Ginseng extractive solution, the Radix Aconiti Lateralis Preparata extracting solution filters respectively, filtrate is respectively by macroporous resin, and with the ethanol elution of 4-6 times of water gagings or 20% following concentration, eluent is abandoned or adopted earlier, reuse adds 1-10 times of eluting of 60-90% ethanol, the collection eluent, decompression recycling ethanol gets the purification Radix Ginseng extractive solution to there not being the alcohol flavor, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution;

G, getting the people and participate in water, is the supersound extraction 3-4 times of 250-800W with ultrasonic power, and each 20-45 minutes, the solvent consumption was 4-6 times of medical material amount; Merge extractive liquid,, being concentrated into 50-60 ℃ of relative densities is 1.1-1.3, adds ethanol and makes concentration of alcohol reach 75%, cold preservation 10-48 hours, filter, reclaim ethanol, add ethanol and make concentration of alcohol reach 85% to there not being the alcohol flavor, cold preservation 24-36 hours filters to clarification, gets the Radix Ginseng extractive solution; Get Radix Aconiti Lateralis Preparata and add 60-80% ethanol, reflux, extract, 3-4 times, each 1-2 hours, the solvent consumption was 4-6 times of medical material amount, merge extractive liquid, gets the Radix Aconiti Lateralis Preparata extracting solution; Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is passed through macroporous resin respectively, first ethanol elution with 4-6 times of water gagings or 20% following concentration, eluent is abandoned or adopted, reuse adds 1-10 times of eluting of 60-90% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution;

H, getting the people and participate in water, is the supersound extraction 3-4 times of 250-800W with ultrasonic power, and each 20-45 minutes, the solvent consumption was 4-6 times of medical material amount; Merge extractive liquid,, being concentrated into 50-60 ℃ of relative densities is 1.1-1.3, adds ethanol and makes concentration of alcohol reach 75%, cold preservation 10-48 hours, filter, reclaim ethanol, add ethanol and make concentration of alcohol reach 85% to there not being the alcohol flavor, cold preservation 24-36 hours filters to clarification, gets the Radix Ginseng extractive solution; Getting Radix Aconiti Lateralis Preparata adds water or concentrated hydrochloric acid to regulate pH is 2-3 sour water, decoct and extract 3-4 times, each 1-2 hours, the solvent consumption is 4-6 times of medical material amount, merge extractive liquid,, being concentrated into 50-60 ℃ of relative densities is 1.1-1.3, adding ethanol makes concentration of alcohol reach 70%, cold preservation 24-36 hours filters, and reclaims ethanol to there not being the alcohol flavor, adding ethanol makes concentration of alcohol reach 80%, cold preservation 24-36 hours filters to clarification, reclaims ethanol to there not being the ethanol flavor, get the Radix Aconiti Lateralis Preparata extracting solution with above-mentioned Radix Ginseng extractive solution, the Radix Aconiti Lateralis Preparata extracting solution filters respectively, filtrate is respectively by macroporous resin, and with the ethanol elution of 4-6 times of water gagings or 20% following concentration, eluent is abandoned or adopted earlier, reuse adds 1-10 times of eluting of 60-90% ethanol, the collection eluent, decompression recycling ethanol gets the purification Radix Ginseng extractive solution to there not being the alcohol flavor, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution;

I, get Radix Ginseng with water extraction 2-3 times, each 1-2 hours, the solvent consumption was 4-6 times of medical material amount; Merge extractive liquid,, being concentrated into 50-60 ℃ of relative densities is 1.1-1.3, adds ethanol and makes concentration of alcohol reach 75%, and cold preservation 10-48 hours filters, and adds ethanol and makes concentration of alcohol reach 85%, and cold preservation 24-36 hours filters to clarification, must go into to join extracting solution; Getting Radix Aconiti Lateralis Preparata and add 60-80% ethanol, is the supersound extraction 3-4 times of 250-800W with ultrasonic power, and each 20-45 minutes, the solvent consumption was 4-6 times of medical material amount, merge extractive liquid,, the Radix Aconiti Lateralis Preparata extracting solution; Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is passed through macroporous resin respectively, first ethanol elution with 4-6 times of water gagings or 20% following concentration, eluent is abandoned or adopted, reuse adds 1-10 times of eluting of 60-90% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution;

J, get Radix Ginseng with water extraction 2-3 times, each 1-2 hours, the solvent consumption was 4-6 times of medical material amount; Merge extractive liquid,, being concentrated into 50-60 ℃ of relative densities is 1.1-1.3, add ethanol and make concentration of alcohol reach 75%, cold preservation 10-48 hours, filtration adds ethanol and makes concentration of alcohol reach 85%, and cold preservation 24-36 hours filters to clarification, gets the Radix Ginseng extractive solution; Getting Radix Aconiti Lateralis Preparata adds water or concentrated hydrochloric acid to regulate pH is 2-3 sour water, with ultrasonic power is the supersound extraction 3-4 times of 250-800W, each 20-45 minutes, the solvent consumption is 4-6 times of medical material amount, merge extractive liquid,, being concentrated into 50-60 ℃ of relative densities is 1.1-1.3, add ethanol and make concentration of alcohol reach 70%, cold preservation 24-36 hours filters, reclaim ethanol to there not being the alcohol flavor, add ethanol and make concentration of alcohol reach 80%, cold preservation 24-36 hours filters to clarification, reclaim ethanol to there not being the ethanol flavor, get the Radix Aconiti Lateralis Preparata extracting solution; Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is passed through macroporous resin respectively, first ethanol elution with 4-6 times of water gagings or 20% following concentration, eluent is abandoned or adopted, reuse adds 1-10 times of eluting of 60-90% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution;

K, get Radix Ginseng with water extraction 2-3 times, each 1-2 hours, the solvent consumption was 4-6 times of medical material amount; Merge extractive liquid,, being concentrated into 50-60 ℃ of relative densities is 1.1-1.3, add ethanol and make concentration of alcohol reach 75%, cold preservation 10-48 hours, filtration adds ethanol and makes concentration of alcohol reach 85%, and cold preservation 24-36 hours filters to clarification, gets the Radix Ginseng extractive solution; Get Radix Aconiti Lateralis Preparata and add 60-80% ethanol, reflux, extract, 3-4 times, each 1-2 hours, the solvent consumption was 4-6 times of medical material amount, merge extractive liquid, gets the Radix Aconiti Lateralis Preparata extracting solution; Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is passed through macroporous resin respectively, first ethanol elution with 4-6 times of water gagings or 20% following concentration, eluent is abandoned or adopted, reuse adds 1-10 times of eluting of 60-90% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution;

L, get Radix Ginseng with water extraction 2-3 times, each 1-2 hours, the solvent consumption was 4-6 times of medical material amount; Merge extractive liquid,, being concentrated into 50-60 ℃ of relative densities is 1.1-1.3, add ethanol and make concentration of alcohol reach 75%, cold preservation 10-48 hours, filtration adds ethanol and makes concentration of alcohol reach 85%, and cold preservation 24-36 hours filters to clarification, gets the Radix Ginseng extractive solution; Get Radix Aconiti Lateralis Preparata and add water or concentrated hydrochloric acid to regulate pH be 2-3 sour water, decoct and extract each 1-2 hours 3-4 times, the solvent consumption is 4-6 times of medical material amount, merge extractive liquid,, and being concentrated into 50-60 ℃ of relative densities is 1.1-1.3, add ethanol and make concentration of alcohol reach 70%, cold preservation 24-36 hours filters, reclaim ethanol to there not being the alcohol flavor, add ethanol and make concentration of alcohol reach 80%, cold preservation 24-36 hours filters to clarification, reclaim ethanol to there not being the ethanol flavor, get the Radix Aconiti Lateralis Preparata extracting solution; Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is passed through macroporous resin respectively, first ethanol elution with 4-6 times of water gagings or 20% following concentration, eluent is abandoned or adopted, reuse adds 1-10 times of eluting of 60-90% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution;

M, get the people and participate in 60-80% ethanol, reflux, extract, 3-4 times, each 1-2 hours, the solvent consumption was 4-6 times of medical material amount, merge extractive liquid,, the Radix Ginseng extractive solution; Getting Radix Aconiti Lateralis Preparata and add 60-80% ethanol, is the supersound extraction 3-4 times of 250-800W with ultrasonic power, each 20-45 minutes, and the solvent consumption? be 4-6 times of the medical material amount, merge extractive liquid, gets the Radix Aconiti Lateralis Preparata extracting solution; Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is passed through macroporous resin respectively, first ethanol elution with 4-6 times of water gagings or 20% following concentration, eluent is abandoned or adopted, reuse adds 1-10 times of eluting of 60-90% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution;

N, get the people and participate in 60-80% ethanol, reflux, extract, 3-4 times, each 1-2 hours, the solvent consumption was 4-6 times of medical material amount, merge extractive liquid,, the Radix Ginseng extractive solution; Getting Radix Aconiti Lateralis Preparata adds water or concentrated hydrochloric acid to regulate pH is 2-3 sour water, with ultrasonic power is the supersound extraction 3-4 times of 250-800W, each 20-45 minutes, the solvent consumption is 4-6 times of medical material amount, merge extractive liquid,, being concentrated into 50-60 ℃ of relative densities is 1.1-1.3, add ethanol and make concentration of alcohol reach 70%, cold preservation 24-36 hours filters, reclaim ethanol to there not being the alcohol flavor, add ethanol and make concentration of alcohol reach 80%, cold preservation 24-36 hours filters to clarification, reclaim ethanol to there not being the ethanol flavor, get the Radix Aconiti Lateralis Preparata extracting solution; Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is passed through macroporous resin respectively, first ethanol elution with 4-6 times of water gagings or 20% following concentration, eluent is abandoned or adopted, reuse adds 1-10 times of eluting of 60-90% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution;

0, get the people and participate in 60-80% ethanol, reflux, extract, 3-4 times, each 1-2 hours, the solvent consumption was 4-6 times of medical material amount, merge extractive liquid, gets the Radix Ginseng extractive solution; Get Radix Aconiti Lateralis Preparata and add water or concentrated hydrochloric acid to regulate pH be 2-3 sour water, decoct and extract each 1-2 hours 3-4 times, the solvent consumption is 4-6 times of medical material amount, merge extractive liquid,, and being concentrated into 50-60 ℃ of relative densities is 1.1-1.3, add ethanol and make concentration of alcohol reach 70%, cold preservation 24-36 hours filters, reclaim ethanol to there not being the alcohol flavor, add ethanol and make concentration of alcohol reach 80%, cold preservation 24-36 hours filters to clarification, reclaim ethanol to there not being the ethanol flavor, get the Radix Aconiti Lateralis Preparata extracting solution; Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is passed through macroporous resin respectively, first ethanol elution with 4-6 times of water gagings or 20% following concentration, eluent is abandoned or adopted, reuse adds 1-10 times of eluting of 60-90% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution.

7. the preparation method of the attached high concentration injection formulation of ginseng as claimed in claim 6 is characterized in that described macroporous resin column model is D101, D201, D601, any one among the HP-20.

8. the preparation method of the attached high concentration injection formulation of ginseng as claimed in claim 7 is characterized in that the preparation method of raw material Radix Ginseng extract and Radix Aconiti Lateralis Preparata extract is prepared by following any one method:

A, get the people and participate in 80% ethanol, supersound extraction 4 times, ultrasonic power is 400W, is respectively 30,30,25,25 minutes, the solvent consumption is 6 times of medical material amount, merge extractive liquid, reclaims ethanol to there being the ethanol flavor, the Radix Ginseng extractive solution; Get Radix Aconiti Lateralis Preparata and add 80% ethanol, supersound extraction 4 times, ultrasonic power is 400W, is respectively 30,30,25,25 minutes, and the solvent consumption is 6 times of medical material amount, and merge extractive liquid, gets the Radix Aconiti Lateralis Preparata extracting solution; Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is respectively the macroporous resin of D101 by model, earlier with 4 times of water gaging eluting, eluent is abandoned or adopted, reuse adds 5 times of eluting of 65% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution;

B, get the people and participate in 60% ethanol, supersound extraction 3 times, ultrasonic power 500W was respectively 30,30,25 minutes, the solvent consumption is 4 times of medical material amount, merge extractive liquid,, the Radix Ginseng extractive solution; Get Radix Aconiti Lateralis Preparata and add concentrated hydrochloric acid to regulate pH be 2-3 sour water, supersound extraction 3 times, ultrasonic power is 500W, be respectively 30,30,25 minutes, the solvent consumption is 4-6 times of medical material amount, merge extractive liquid,, being concentrated into 60 ℃ of relative densities is 1.25, adds ethanol and makes concentration of alcohol reach 70%, cold preservation 24 hours, filter, reclaim ethanol, add ethanol and make concentration of alcohol reach 80% to there not being the alcohol flavor, cold preservation 24 hours, filtration is reclaimed ethanol to there being the ethanol flavor to clarification, gets the Radix Aconiti Lateralis Preparata extracting solution; Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is respectively the macroporous resin of D601 by model, earlier with 5 times of water gaging eluting, eluent is abandoned or adopted, reuse adds 10 times of eluting of 60% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution;

C, get the people and participate in 70% ethanol, supersound extraction 3 times, ultrasonic power is 500W, is respectively 30,25,25 minutes, the solvent consumption is 5 times of medical material amount, merge extractive liquid,, the Radix Ginseng extractive solution; Get Radix Aconiti Lateralis Preparata and add 70% ethanol, reflux, extract, 4 times was respectively 2,2,1,1 hours, and the solvent consumption is 5 times of medical material amount, and merge extractive liquid, gets the Radix Aconiti Lateralis Preparata extracting solution; Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is the D601 macroporous resin by model respectively, measure 12% ethanol elution with 4 times earlier, eluent is abandoned or adopted, reuse adds 6 times of eluting of 75% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution;

D, get the people and participate in 75% ethanol, supersound extraction 3 times, ultrasonic power is 600W, is respectively 30,25,25 minutes, the solvent consumption is 6 times of medical material amount, merge extractive liquid,, the Radix Ginseng extractive solution; Getting Radix Aconiti Lateralis Preparata, to add pH be 2 the sour water of regulating with concentrated hydrochloric acid, decocts and extract 3 times, was respectively 2,1,1 hours, the solvent consumption is 4.5 times of medical material amount, merge extractive liquid,, and being concentrated into 50 ℃ of relative densities is 1.1, add ethanol and make concentration of alcohol reach 70%, cold preservation 26 hours filters, reclaim ethanol to there not being the alcohol flavor, add ethanol and make concentration of alcohol reach 80%, cold preservation 30 hours filters to clarification, reclaim ethanol to there not being the ethanol flavor, get the Radix Aconiti Lateralis Preparata extracting solution; Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is HP-20 macroporous resin by model respectively, earlier with 5 times of water gaging eluting, eluent is abandoned or adopted, reuse adds 5 times of eluting of 70% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution;

E, get the people and participate in water, supersound extraction 3 times, ultrasonic power is 700W, is respectively 35,30,20 minutes, the solvent consumption is 5 times of medical material amount; Merge extractive liquid,, being concentrated into 60 ℃ of relative densities is 1.2, add ethanol and make concentration of alcohol reach 75%, cold preservation 24 hours, filtration is reclaimed ethanol and is not distinguished the flavor of to there being alcohol, adds ethanol and makes concentration of alcohol reach 85%, and cold preservation 28 hours filters to clarification, gets the Radix Ginseng extractive solution; Get Radix Aconiti Lateralis Preparata and add 80% ethanol, supersound extraction 3 times, ultrasonic power is 800W, is respectively 25,20,20 minutes, and the solvent consumption is 6 times of medical material amount, and merge extractive liquid, gets the Radix Aconiti Lateralis Preparata extracting solution; Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is the D101 macroporous resin by model respectively, first ethanol elution with 4-6 times of water gagings or 20% following concentration, eluent is abandoned or adopted, reuse adds 2 times of eluting of 85% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution;

F, get the people and participate in water, supersound extraction 3 times, ultrasonic power is 800W, is respectively 30,30,20 minutes, the solvent consumption is 5 times of medical material amount; Merge extractive liquid,, being concentrated into 60 ℃ of relative densities is 1.3, add ethanol and make concentration of alcohol reach 75%, cold preservation 20 hours, filtration is reclaimed ethanol and is not distinguished the flavor of to there being alcohol, adds ethanol and makes concentration of alcohol reach 85%, and cold preservation 30 hours filters to clarification, gets the Radix Ginseng extractive solution; Get Radix Aconiti Lateralis Preparata and add water, supersound extraction 3 times, ultrasonic power is 800W, is respectively 30,30,20 minutes, the solvent consumption was 6 times of medical material amount, merge extractive liquid,, being concentrated into 50 ℃ of relative densities is 1.1, adds ethanol and makes concentration of alcohol reach 70%, cold preservation 30 hours, filter, reclaim ethanol to there not being the alcohol flavor, add ethanol and make concentration of alcohol reach 80%, cold preservation 30 hours, filter to clarification, reclaim ethanol to there not being the ethanol flavor, get the Radix Aconiti Lateralis Preparata extracting solution above-mentioned Radix Ginseng extractive solution, the Radix Aconiti Lateralis Preparata extracting solution filters respectively, and filtrate is the D601 macroporous resin by model respectively, measure 18% ethanol elution with 4 times earlier, eluent is abandoned or adopted, and reuse adds 6 times of eluting of 70% ethanol, collects eluent, decompression recycling ethanol gets the purification Radix Ginseng extractive solution to there not being the alcohol flavor, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution;

G, get the people and participate in water, supersound extraction 3 times, ultrasonic power is 700W, is respectively 30,30,20 minutes, the solvent consumption is 6 times of medical material amount; Merge extractive liquid,, being concentrated into 55 ℃ of relative densities is 1.15, add ethanol and make concentration of alcohol reach 75%, cold preservation 18 hours, filtration is reclaimed ethanol and is not distinguished the flavor of to there being alcohol, adds ethanol and makes concentration of alcohol reach 85%, and cold preservation 30 hours filters to clarification, gets the Radix Ginseng extractive solution; Get Radix Aconiti Lateralis Preparata and add 80% ethanol, reflux, extract, 3 times was respectively 2,1,1 hours, and the solvent consumption is 6 times of medical material amount, and merge extractive liquid, gets the Radix Aconiti Lateralis Preparata extracting solution; Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is the D201 macroporous resin by model respectively, measure 10% ethanol elution with 5 times earlier, eluent is abandoned or adopted, reuse adds 3 times of eluting of 85% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution;

H, get the people and participate in water, supersound extraction 4 times, ultrasonic power is 300W, is respectively 40,40,30,20 minutes, the solvent consumption is 6 times of medical material amount; Merge extractive liquid,, being concentrated into 60 ℃ of relative densities is 1.2, add ethanol and make concentration of alcohol reach 75%, cold preservation 20 hours, filtration is reclaimed ethanol and is not distinguished the flavor of to there being alcohol, adds ethanol and makes concentration of alcohol reach 85%, and cold preservation 30 hours filters to clarification, gets the Radix Ginseng extractive solution; Get Radix Aconiti Lateralis Preparata and add that to adopt concentrated hydrochloric acid to be adjusted to pH be 3 sour water, decoct and extract 3 times, be respectively 2,2,1 hour, the solvent consumption is 4 times of medical material amount, merge extractive liquid,, and being concentrated into 55 ℃ of relative densities is 1.3, adding ethanol makes concentration of alcohol reach 70%, cold preservation 24 hours filters, and reclaims ethanol to there not being the alcohol flavor, adding ethanol makes concentration of alcohol reach 80%, cold preservation 32 hours filters to clarification, reclaims ethanol to there not being the ethanol flavor, get the Radix Aconiti Lateralis Preparata extracting solution with above-mentioned Radix Ginseng extractive solution, the Radix Aconiti Lateralis Preparata extracting solution filters respectively, filtrate is respectively the macroporous resin of D601 by model, and with 6 times of water gaging eluting, eluent is abandoned or adopted earlier, reuse adds 2 times of eluting of 85% ethanol, the collection eluent, decompression recycling ethanol gets the purification Radix Ginseng extractive solution to there not being the alcohol flavor, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution;

I, get Radix Ginseng with water extraction 3 times, each 2 hours, the solvent consumption was 4 times of medical material amount; Merge extractive liquid,, being concentrated into 60 ℃ of relative densities is 1.3, add ethanol and make concentration of alcohol reach 75%, cold preservation 19 hours, filtration adds ethanol and makes concentration of alcohol reach 85%, and cold preservation 28 hours filters to clarification, gets the Radix Ginseng extractive solution; Get Radix Aconiti Lateralis Preparata and add 80% ethanol, supersound extraction 3 times, ultrasonic power is 700W, is respectively 30,30,20 minutes, and the solvent consumption is 5.5 times of medical material amount, and merge extractive liquid, gets the Radix Aconiti Lateralis Preparata extracting solution; Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is the D601 macroporous resin by model respectively, earlier with 6 times of water gaging eluting, eluent is abandoned or adopted, reuse adds 8 times of eluting of 65% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution;

J, get Radix Ginseng with water extraction 2 times, each 2 hours, the solvent consumption was 5 times of medical material amount; Merge extractive liquid,, being concentrated into 55 ℃ of relative densities is 1.25, add ethanol and make concentration of alcohol reach 75%, cold preservation 17 hours, filtration adds ethanol and makes concentration of alcohol reach 85%, and cold preservation 34 hours filters to clarification, gets the Radix Ginseng extractive solution; Get Radix Aconiti Lateralis Preparata and add water, supersound extraction 3 times, ultrasonic power is 750W, each 30,25,20 minutes, the solvent consumption was 6 times of medical material amount, merge extractive liquid,, being concentrated into 55 ℃ of relative densities is 1.15, adds ethanol and makes concentration of alcohol reach 70%, cold preservation 36 hours, filter, reclaim ethanol, add ethanol and make concentration of alcohol reach 80% to there not being the alcohol flavor, cold preservation 30 hours, filtration is reclaimed ethanol to there being the ethanol flavor to clarification, gets the Radix Aconiti Lateralis Preparata extracting solution; Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is the D601 macroporous resin by model respectively, measure 12% ethanol elution with 4 times earlier, eluent is abandoned or adopted, reuse adds 4 times of eluting of 75% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution;

K, get Radix Ginseng with water extraction 3 times, be respectively 2,2,1 hours, the solvent consumption is 6 times of medical material amount; Merge extractive liquid,, being concentrated into 60 ℃ of relative densities is 1.1, add ethanol and make concentration of alcohol reach 75%, cold preservation 28 hours, filtration adds ethanol and makes concentration of alcohol reach 85%, and cold preservation 30 hours filters to clarification, gets the Radix Ginseng extractive solution; Get Radix Aconiti Lateralis Preparata and add 75% ethanol, reflux, extract, 3 times, each 1.5 hours, the solvent consumption was 5 times of medical material amount, merge extractive liquid, gets the Radix Aconiti Lateralis Preparata extracting solution; Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is the D101 macroporous resin by model respectively, first ethanol elution with 4-6 times of water gagings or 20% following concentration, eluent is abandoned or adopted, reuse adds 1-10 times of eluting of 60-90% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution;

L, get Radix Ginseng with water extraction 3 times, be respectively 2,2,1 hours, the solvent consumption is 6 times of medical material amount; Merge extractive liquid,, being concentrated into 50 ℃ of relative densities is 1.15, add ethanol and make concentration of alcohol reach 75%, cold preservation 35 hours, filtration adds ethanol and makes concentration of alcohol reach 85%, and cold preservation 26 hours filters to clarification, gets the Radix Ginseng extractive solution; Get Radix Aconiti Lateralis Preparata and add the sour water that adopts concentrated hydrochloric acid to be adjusted to pH=3, decoct and extract 3 times, be respectively 2,1,1 hours, the solvent consumption is 6 times of medical material amount, merge extractive liquid,, and being concentrated into 60 ℃ of relative densities is 1.3, add ethanol and make concentration of alcohol reach 70%, cold preservation 30 hours filters, reclaim ethanol to there not being the alcohol flavor, add ethanol and make concentration of alcohol reach 80%, cold preservation 32 hours filters to clarification, reclaim ethanol to there not being the ethanol flavor, get the Radix Aconiti Lateralis Preparata extracting solution; Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is HP-20 macroporous resin by model respectively, earlier with 5 times of water gaging eluting, eluent is abandoned or adopted, reuse adds 4 times of eluting of 70% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution;

M, get the people and participate in 60% ethanol, reflux, extract, 4 times, each 1.5 hours, the solvent consumption was 6 times of medical material amount, merge extractive liquid,, the Radix Ginseng extractive solution; Get Radix Aconiti Lateralis Preparata and add 75% ethanol, supersound extraction 3 times, ultrasonic power is 600W, is respectively 40,30,30 minutes, and the solvent consumption is 6 times of medical material amount, and merge extractive liquid, gets the Radix Aconiti Lateralis Preparata extracting solution; Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is the D201 macroporous resin by model respectively, earlier with 6 times of water gaging eluting, eluent is abandoned or adopted, reuse adds 5 times of eluting of 85% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution;

N, get the people and participate in 80% ethanol, reflux, extract, 3 times was respectively 2,1,1 hours, and the solvent consumption is 4 times of medical material amount, merge extractive liquid,, the Radix Ginseng extractive solution; Get Radix Aconiti Lateralis Preparata and add water, supersound extraction 3 times, ultrasonic power is 800W, be respectively 35,30,20 minutes, the solvent consumption is 6 times of medical material amount, merge extractive liquid,, being concentrated into 60 ℃ of relative densities is 1.3, adds ethanol and makes concentration of alcohol reach 70%, cold preservation 24 hours, filter, reclaim ethanol, add ethanol and make concentration of alcohol reach 80% to there not being the alcohol flavor, cold preservation 26 hours, filtration is reclaimed ethanol to there being the ethanol flavor to clarification, gets the Radix Aconiti Lateralis Preparata extracting solution; Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is the D601 macroporous resin by model respectively, measure 15% ethanol elution with 6 times earlier, eluent is abandoned or adopted, reuse adds 9 times of eluting of 65% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution;

O, get the people and participate in 65% ethanol, reflux, extract, 4 times, each 2 hours, the solvent consumption was 6 times of medical material amount, merge extractive liquid,, the Radix Ginseng extractive solution; Get Radix Aconiti Lateralis Preparata and add the sour water that adopts concentrated hydrochloric acid to be adjusted to pH=2.5, decoct and extract each 1.5 hours 3 times, the solvent consumption is 5 times of medical material amount, merge extractive liquid,, and being concentrated into 60 ℃ of relative densities is 1.25, add ethanol and make concentration of alcohol reach 70%, cold preservation 26 hours filters, reclaim ethanol to there not being the alcohol flavor, add ethanol and make concentration of alcohol reach 80%, cold preservation 34 hours filters to clarification, reclaim ethanol to there not being the ethanol flavor, get the Radix Aconiti Lateralis Preparata extracting solution; Above-mentioned Radix Ginseng extractive solution, Radix Aconiti Lateralis Preparata extracting solution are filtered respectively, filtrate is HP-20 macroporous resin by model respectively, earlier with 6 times of ethanol elutions of measuring 18% following concentration, eluent is abandoned or adopted, reuse adds 7 times of eluting of 75% ethanol, collection eluent, decompression recycling ethanol extremely do not have the alcohol flavor, get purification Radix Ginseng extractive solution, dry Radix Ginseng or the Radix Aconiti Lateralis Preparata extract of getting of purification Radix Aconiti Lateralis Preparata extracting solution.

9. as the preparation method of the attached high concentration injection formulation of the described ginseng of one of claim 6-8, it is characterized in that the medicinal liquid of spraying in the spray drying is 1.1-1.2 60-70 ℃ of following relative densities, containing amount of solid is 30-60%; 130-180 ℃ of drying tower inlet temperature, leaving air temp is 90-110 ℃ at the bottom of the tower.

10. as the preparation method of the attached high concentration injection formulation of the described ginseng of one of claim 6-8, it is characterized in that in the lyophilization that thickness is no more than 10-20mm in the cryodesiccated container,-30--35 ℃ of 12-14 hours freezing times, taking out container, to put into freezer dryer drying time be 20-26 hours.

11. preparation method as the attached high concentration injection formulation of the described ginseng of one of claim 6-8, it is characterized in that the Radix Ginseng extract that makes or Radix Aconiti Lateralis Preparata extract adopt membrane separation technique further refining, any that the model of used film is FLT-Z (II)--FLT-VL, FLT-Z (II)--among-FLT-VG---FLT-VAB---FLT-VAC, the FLT-VL.