CN100494346C - Application of anti-chlorine strain V430 - Google Patents
Application of anti-chlorine strain V430 Download PDFInfo
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- CN100494346C CN100494346C CNB2006100181322A CN200610018132A CN100494346C CN 100494346 C CN100494346 C CN 100494346C CN B2006100181322 A CNB2006100181322 A CN B2006100181322A CN 200610018132 A CN200610018132 A CN 200610018132A CN 100494346 C CN100494346 C CN 100494346C
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Abstract
The present invention relates to the application of chlorine tolerant microbe, and is especially the application of Staphylococcus saprophyticusV430 CCTCC No.M205145 as a kind of chlorine tolerant microbe for treating waste water with high chlorine ion concentration. The treating method includes sorting Staphylococcus saprophyticusV430 single colony, culturing in enlarging culture medium for 20-24 hr, inoculating the cultured Staphylococcus saprophyticusV430in certain amount into waste water with high chlorine ion concentration, and constant temperature anaerobic culturing and reaction at 25-45 deg.c and pH 7.0-8.0 for 3-5 days. The present invention has high COD eliminating capacity and can eliminate COD in 50-60 % from waste water in 5 days.
Description
Technical field
The present invention relates to a kind of anti-chlorine application of microorganism.
Background technology
The high salinity organic wastewater with difficult degradation thereby has become the worldwide technological puzzle of environmental protection water treatment field as waste water such as oil production, chemical industry, pharmacy.And salt acid system turmeric saponin factory effluent is representative in high density saliferous hardly degraded organic substance waste water.On the one hand, this class waste water complicated component, the organism of difficult degradation is many, and the biodegradability of waste water is poor; On the other hand, the salts contg height in the waste water, chlorine ion concentration is big, and chlorine ion concentration can reach 20000mg/L in its composite waste.At present, salt-containing organic wastewater adopts abiotic method and biological method, abiotic method is owing to its processing costs height, treatment effect are bad, enterprise can't accept, biochemical process is the at first method in the water technology always, the main two sections contact oxidation methods of A-B that adopt, traditional activated sludge process, the SBR method, biological filter, anaerobic filter etc., it is waste water below 3% that but above-mentioned biological process can only be handled saltiness mostly, and high-salt wastewater (saltiness 5%, even 20%) is difficult to handle, therefore need special microorganism.In recent years, reported that abroad relevant seed selection salt tolerant (NaCl) bacterium handles the report of high salt organic waste water, and to this class high-enriched organics content of saponin waste water, high salt (CaCl2), contain the complicated waste water of hard-degraded substance (saponin), also belong to the weak field of research so far.
Summary of the invention
The object of the present invention is to provide the application of a chlorine-resisting strain V430.
Anti-chlorine strain V 430 provided by the present invention, be Staphylococcus saprophyticus (Staphylococcus saprophyticus) V430 CCTCC NO.M205145, be preserved in Chinese typical culture collection center (being called for short CCTCC) on December 9th, 2005, CCTCC address: Hubei Wuhan-Wuhan University, preserving number: CCTCC NO.M205145.
The application of one chlorine-resisting strain V430 is characterized in that a staphylococcus saprophyticus (Staphylococcus saprophyticus) the V430 CCTCC NO.M205145 that is applied as of described bacterial strain is applied to contain in the processing of high-concentration chlorine ion wastewater.
The treatment process that a described staphylococcus saprophyticus (Staphylococcus saprophyticus) V430 is applied to contain high-concentration chlorine ion wastewater is: the single bacterium colony of picking Staphylococcus saprophyticus (Staphylococcus saprophyticus) V430, in the enlarged culturing base, cultivated 20-24 hour, by Staphylococcus saprophyticus (Staphylococcussaprophyticus) V430 after cultivating: the volume ratio that contains high-concentration chlorine ion wastewater (as mixing saponin waste water) is (1-4): 50 get Staphylococcus saprophyticus (Staphylococcus saprophyticus) V430 is inoculated in and contains in the high-concentration chlorine ion wastewater (as mixing saponin waste water), 25-45 ℃ of constant temperature anaerobism cultivated, the pH value is 7.0-8.0, reacts 3-5 days.
Described enlarged culturing base is: peptone 10g; Extractum carnis 5g; Distilled water 1000ml; CaCl
218.0g/L, pH=7.5.
The present invention screens a strain has the certain growth ability under the high-concentration chlorine ion condition new bacterial strain from the saponin waste water pool bottom sludge.One chlorine-resisting strain V430 has the ability of COD in the good removal saponin waste water, can remove the COD 50-60% in the waste water within 5 days.
Description of drawings
Fig. 1-1a is a strain V 430 bacterial strain Electronic Speculum picture
Fig. 1-1b is a strain V 430 bacterial strain Electronic Speculum picture
Fig. 1-1c is a strain V 430 bacterial strain Electronic Speculum picture
Fig. 1-2 is strain V 430 growing state figure on the SC substratum of the different chlorine ion concentrations of liquid
Fig. 1-the 3rd, and strain V 430 pcr amplification agarose electrophoresis figure (1: nuclear DNA, 2: amplified production, M:Marker)
Fig. 1-4a is the following degraded efficiency diagram of strain V 430 under high chloride ion, high COD concentration
Fig. 1-4b is the following degraded efficiency diagram of strain V 430 under high chloride ion, high COD concentration
Fig. 1-4c is the following degraded efficiency diagram of strain V 430 under high chloride ion, high COD concentration
Fig. 1-4d is the following degraded efficiency diagram of strain V 430 under high chloride ion, high COD concentration
Anti-chlorine strain V 430, be Staphylococcus saprophyticus (Staphylococcus saprophyticus) V430 CCTCCNO.M205145, be preserved in Chinese typical culture collection center (being called for short CCTCC) on December 9th, 2005, CCTCC address: Hubei Wuhan-Wuhan University, preserving number: CCTCC NO.M205145.
Embodiment
One, the screening method of a chlorine-resisting strain V430:
(1), material is prepared:
1, bacterium source and experiment waste water:
(1) bacterium source: the active sludge in the biochemical system of processing saponin waste water;
(2) experiment waste water (promptly mixing saponin waste water): this experiment waste water is taken from the female Home Co., Ltd in Shiyan City side, Hubei Province diosgenin wastewater; After interior electrolysis and CaO allotment, specific targets are: pH=7.5-8.2, COD=4000-17000mg/L, Cl
-=8000-30000mg/L, NH
4-N 140-300mg/L.
2, substratum:
Separate (fully) substratum: peptone 10g; Extractum carnis 5g; Sodium-chlor; 5g; Distilled water 1000ml.
Screening culture medium: MgSO47H
2O, 0.2g/L, KH
2PO
40.5g/L, K
2HPO
41.5g/L, NH
4Cl 0.5/L, CaCl
2135g/L, the 1ml trace element solution dissolves in 1L saponin waste water (COD concentration is 1000mg/L), pH=7.5;
Trace element solution: FeCl
36H
2O 2g, CoCl
22g, MnCl
20.5g, CuCl
20.03g, ZnCl
20.05g, NiCl
2.6H
2O 0.05g, EDTA 1g, pH=7.0.
Enlarged culturing base: peptone 10g; Extractum carnis 5g; Sodium-chlor 5g; Distilled water 1000ml; CaCl
218.0g/L.
SC substratum: peptone 10g; Extractum carnis 5g; Sodium-chlor 5g; Distilled water 1000ml; CaCl
2Add-on is 12.6g-140g; Work as CaCl
2When add-on is 12.6g, [Cl-]=10000mg/L in the SC substratum.
Slant medium: peptone 10g; Extractum carnis 5g; Sodium-chlor; 5g; Potassium primary phosphate 2g; Ammonium chloride 1g; Agar 20g; Distilled water 1000ml; CaCl
218g/L, pH=7.5.
Above substratum is standby after respectively at 121 ℃ of autoclaving 30min.
3, laboratory apparatus and equipment:
State China SHA-B constant temperature oscillator,
SHH150G illumination bio-incubator,
Horizontal pressure steam sterilizer,
WMX-1 type microwave seal is cleared up the COD tacheometer
The 722S spectrophotometer,
Opticmicroscope,
PH meter,
Bechtop,
Air dry oven,
Ultramicrotome,
The H-7000FA of Hitachi projection microscope,
PTC200 type PCR instrument,
Electrophoresis apparatus and electrophoresis chamber,
Gel ultraviolet visualizer etc.
(2), the separation of bacterial strain and screening:
1, zoogleic fragmentation:
Get the anaerobic activated sludge in the 2g saponin waste water treatment system, add in the triangular flask of the 250ml that the 100ml sterilized water is housed that sterilization is good in advance, and adding weight is the trisodium phosphate of sterilized water 0.01%, the shaking table vibration, zoogloea is smashed, got mud mixture, standby.
2, the separation of anti-chlorine ion dominant bacteria:
Utilize the above-mentioned mud mixture of aseptic pipette, extract 0.5ml, add isolation medium 4.5ml, 35 ℃ of anaerobism constant temperature culture 5-7 days, treat that the test tube substratum becomes muddy, illustrate that bacterium grows, dull and stereotyped then dilution coating, picking has single bacterium of notable difference on colony characteristics, until obtaining single bacterial strain, and preserve in the slant medium ramp.
3, the screening of anti-chlorine ion dominant bacteria:
The above-mentioned isolated single bacterium colony of picking is inoculated in respectively in the screening culture medium, 35 ℃ of anaerobism constant temperature culture 5-7 days, and the muddy situation of observing bacteria suspension becomes the muddy high chlorine resistant ionic bacterial strain that filters out that is.Through screening, obtain the bacterial strain that a strain is numbered strain V 430 at last, i.e. anti-chlorine strain V 430.Anti-chlorine strain V 430 growing state on the liquid SC substratum that adds the different concns chlorion (is aided with [Cl simultaneously shown in Fig. 1-2
-OD during]=3000mg/L
600nmBe control value).
Two, the microorganism strains of anti-chlorine is identified:
1, to the evaluation of the stronger strain V 430 of chlorine-resistant property:
The stronger strain V 430 of chlorine-resistant property is carried out the evaluation of Physiology and biochemistry and the evaluation of 16S rDNA molecule, determined the kind of chlorine resisting strain from molecular level.
16S rDNA sequential analysis is mainly according to following steps:
1. the extraction of bacterium nuclear DNA,
1) choose single colony inoculation overnight incubation in LB liquid pipe with autoclaved toothpick, get 1.5ml bacterium liquid in the Eppendorf pipe, 8, the centrifugal 5min of 000rpm room temperature thoroughly removes supernatant.
2) add STE solution 1.5ml washing once, the centrifugal supernatant of abandoning adds 0.6mlTE solution again, resuspended bacterium.
3) add the N,O-Diacetylmuramidase of 30ul10mg/ml, 37 ℃ of water-bath 45min,
4) add the SDS of 65 μ l 10%, the Proteinase K 3 μ l of 20mg/ml, 50 ℃ of water-baths 2 hours, clear to solution becomes.
5) add equivalance body phenol chloroform extracting three times, till can't see egg white layer.
6) NaAc (pH5.2) of the 3M of adding 1/10 volume in supernatant liquor.
7) add isopyknic Virahol, deposit D NA.Twine with glass stick and to choose the DNA filament, once with 70% washing with alcohol, natural airing, and DNA is dissolved in the 100 μ l TE solution.
8) adding final concentration is the RNase of 50 μ g/ml, and 37 ℃, 30min removes RNA, and-20 ℃ of preservations are standby.
2. the pcr amplification of 16S rDNA gene
P1: forward primer 5 ' AGAGTTTGATCCTGGCTCAG3 '
P2: reverse primer 5 ' GGTTACCTTGTTACGACTT3 '
In 50 μ L reaction volumes, add 1 μ L template DNA (0.1 μ g), 0.5 μ L P1 and P2 (final concentration is 0.5 μ M), 1 μ LdNTP (every kind of NTP0.2mM), 0.5 μ LTaq polysaccharase (2U) and 5 μ L, 10 * PCR damping fluid.The pcr amplification condition is: 94 ℃ of pre-sex change 5min; At 94 ℃ of sex change 30s, 61-65 ℃ of annealing 30s, 72 ℃ are extended 1min, circulate 30 times; Last 72 ℃ are extended 10min eventually.
3. the recovery of PCR product
After the PCR product carried out electrophoresis with 1% sepharose, under ultraviolet lamp, cut and contain desire and reclaim segmental gel, put into the 1.5ml centrifuge tube and add 2 times of volume TE, 65 ℃ of water-baths add the extracting of equal-volume water-saturated phenol and get the upper strata water after once centrifugal and use that phenol-chloroform-the primary isoamyl alcohol extracting once again after 10 minutes, reset and add 10mol/L ammonium acetate and 2 times of volume dehydrated alcohol precipitations of 0.1 times of volume in the collection, centrifugal, wash precipitation once with 70% ethanol, be dissolved in an amount of sterilization distilled water after air-dry.
4. the complete sequence determination of 16S rDNA and analysis
P-f：CACGACGTTGTAAAACGACAGTTTGATCCTGGCTC；
P-r：GGATAACAATTTCACACAGGAAGGAGGTGATCCAGCC。
Add 1 μ L template DNA (＜0.1 μ g), 30 circulations of pcr amplification (94 ℃ of 1min, 55 ℃ of 30s, 72 ℃ of 2min).Adopt dyestuff terminator termination reaction in the ABI PRISM sequencing kit.Then, on Applied Biosystem 373A dna sequencing instrument, check order.The 16S rDNA sequence that records adopts BLAST software and the comparative analysis of GenBank database, finally determines the kind of this bacterium from molecular level.
2,16S rDNA sequencing:
The present invention adopts the method for 16S rDNA sequencing and analysis is carried out the evaluation of molecular level to bacterium.Nuclear DNA with bacterium is a template, is primer with the universal primer of the pcr amplification of 16S rDNA gene, carries out pcr amplification, (detecting) with 1% agarose gel electrophoresis, and as Figure 1-3.After the PCR product is purified, measure its complete sequence.
＜110〉China Geological Univ. Wuhan
＜120〉gene of anti-chlorine strain V 430
<160>1423
<210>1
<211>1423bp
<212>DNA
＜213〉Staphylococcus saprophyticus (Staphylococcus saprophyticus)
<220>
<221>misc_feature
<222>
<400>1
GTTCCATGTGGGTAACCTACCTATAAGACTGTTCTAACTCCGGGATACCGGGGCTAATGC 120
CGGATGGCATTAGAACCGGTTGCCCGGAGTGTGAAAGATGGTTTTGCTATCTCTTATACG 180
TGGACCCGCGCCGTATGAGGTAGTTGGTAAGGTAATGGCTTACCAAGGCAACGATACGTA 240
GCCGACCTGAGAGGGTGATCGGCCACACTGGAACTGAGACACGGTCCAACTCTCTACGGG 300
AGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGACGGAGCAACGCCGCGTGAG 360
TGATGAAGGGTTTCGGCTCGTAAAACTCTGTTATTAGGGAAGAACAAACGTGTAAGTAAC 420
TGTGCACGTCTTGACGGTACCTAATCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGC 480
GGTAATACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGTAGGCGG 540
TTTCTTAAGTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGA 600
GACTTGAGTGCAGAAGAGGAAAGTGGAATTCCATGTGTAGCGGTGAAATGCGCAGAGATA 660
TGGAGGAACACCAGTGGCGAAAGCGACTTTCTGGTCTGTAACTGACGCTGATGTGCGAAA 720
GCGTGGGGATCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTA 780
AGTGTTAGGGGGTTTCCGCCCCTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGG 840
GAGTACGACCGCAAGGTTGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAG 900
CATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAAATCTTGACATCCTTTGACCA 960
CTCTAGAGATAGAGTTTTCCCCTTCGGGGGACAAAGTGACAGGTGGTGCATGGTTGTCGT 1020
CAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTAAACTTAGT 1080
TGCCAGCATTTAGTTGGGCACTCTAGGTTGACTGCCGGTGACAAACCGGAGGAAGGTGGG 1140
TACAAAGGGCAGCTAAACCGCGAGGTCATGCAAATCCCATAAAGTTGTTCTCAGTTCGGA 1260
TTGTAGTCTGCAACTCGACTACATGAAGCTGGAATCGCTAGTAATCGTAGATCAGCATGC 1320
TACGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCACGAGAGTTTGTAA 1380
CACCCGAAGCCGGTGGAGTAACCATTAATGGAGCTAGCCGTCG 1423
Three, the colonial morphology of anti-chlorine strain V 430, physiology and biochemical character see Table 1.Cellular form is seen Fig. 1-1a, Fig. 1-1b, Fig. 1-1c.
Table 1:
The colony morphology characteristic of strain V 430 and main physiological characteristic
Nomenclature in the table: "+": the bacterial strain more than 90% is positive, "-": the bacterial strain more than 90% is negative
Four, strain V 430 is new bacterial strain:
The 16S rDNA gene order of strain V 430 and the sequence in the international GenBank database are carried out online homology find that relatively the homology of a plurality of bacterial strains of bacterium V430 and Staphylococcus saprophyticus (Staphylococcus saprophyticus) is up to more than the 98-99%.The Physiology and biochemistry and the Molecular Identification result of comprehensive strain V 430, can determine that bacterium V430 is Staphylococcus saprophyticus (Staphylococcus saprophyticus), strain V 430 called after Staphylococcus saprophyticus (Staphylococcussaprophyticus) V430 (being anti-chlorine strain V 430), consult pertinent data, do not have still that Staphylococcus saprophyticus belongs to organic wastewater with difficult degradation thereby under the relevant high-concentration chlorine ion condition and the report of its anti-chlorine capability study.Staphylococcus saprophyticus (Staphylococcussaprophyticus) V430 was new bacterial strain, had been preserved in Chinese typical culture collection center (being called for short CCTCC), preserving number: CCTCC NO.M205145 on December 9th, 2005.
The strain V 430 that the present invention separates, filters out has the function of better degrading high concentration chlorion difficult degradation saponin waste water.This has just widened people to the applied research thinking of Staphylococcus saprophyticus (Staphylococcus saprophyticus) in its function aspects, and provide useful bacterium source and technology for degraded contains the high-concentration chlorine ion saponin waste water, have stronger actual application value.
Five, the application of anti-chlorine strain V 430:
Application example 1:
1. in the enlarged culturing base, anti-chlorine strain V 430 is carried out enlarged culturing, get saponin composite waste ([Cl-]=8000-30000/L, COD=4000-17000mg/L) 50ml adds respectively in the Erlenmeyer flask of 250ml, the bacteria suspension 1ml that adds bacterial strain respectively, 35 ℃, pH value=7.0-7.5, anaerobic reaction 5 days calculates the removal situation of bacterial strain to the COD of waste water, the degradation factors experiment is except the factor of being investigated, and all the other are all with above condition.The results are shown in Figure 1-4a, Fig. 1-4b, Fig. 1-4c, Fig. 1-4d.
2. the factorial experiments of anti-chlorine strain V 430 degraded saponin waste water:
9864.2,14980,21061,24780,30080mg/L 1) in order to investigate bacterial strain under different chlorine ion concentrations,, in waste water, add CaCl2 and regulate chlorine ion concentration and be respectively: the removal effect of COD.Anti-chlorine strain V 430 removal effect result such as Fig. 1-4a.
From Fig. 1-4a as can be seen, when [Cl-]=9864.2mg/L, reaction times is 3 days, strain V 430 only is 39.31% to the clearance of COD, but rising along with chlorine ion concentration in the waste water, strain V 430 increases the clearance of COD, when chlorine ion concentration in the waste water reaches 2.5 ten thousand mg/L, strain V 430 can reach 64.38% to the clearance of COD, and strain V 430 increases and changes little to the clearance of COD along with the continuation of chlorine ion concentration in the waste water, when chlorine ion concentration in the waste water during up to 30,000 mg/L, strain V 430 still remains on 60% to the clearance of COD.
2) in order to investigate bacterial strain (6610.4,7906.4,11209.6,13420.0,16196.2mg/L) under different COD concentration,, in waste water, add CaCl to the removal effect of COD
2Regulate chlorine ion concentration and be respectively 20000mg/L.Anti-chlorine strain V 430 removal effect result such as Fig. 1-4b.By experiment as can be known: as [Cl-]=20000mg/L in the waste water, when COD concentration reaches 10,000 mg/L, strain V 430 is better to the clearance of COD, can reach 62.37%.
3) from Fig. 1-4c and 1-4d as can be seen, strain V 430 increases afterwards earlier with the increase of pH the clearance of COD and descends, and its highest clearance reaches 62% when pH is 7.5-8.Waste water after the centrifugal treating, when finding that pH is 7-8, the wet thallus amount is 9,10,11 o'clock apparently higher than pH, as seen during in neutrality, helps the growth of bacterial strain at pH; When inoculum size changed between 1-4ml, strain V 430 did not have considerable change to the clearance of COD, was 60% substantially, and when inoculum size was 1ml, its clearance was best.This is the certain nutritive substance of growth needs because of bacterial strain, and when bacteria suspension was 1ml, its material that needs was met easily.
Described enlarged culturing base is: peptone 10g; Extractum carnis 5g; Distilled water 1000ml; CaCl
218.0g/L, pH=7.5.
Application example 2:
The treatment process that one staphylococcus saprophyticus (Staphylococcus saprophyticus) V430 is applied to contain high-concentration chlorine ion wastewater is: the single bacterium colony of picking Staphylococcus saprophyticus (Staphylococcus saprophyticus) V430, in the enlarged culturing base, cultivated 20 hours, by Staphylococcus saprophyticus (Staphylococcus saprophyticus) V430 after cultivating: the volume ratio that contains high-concentration chlorine ion wastewater (as mixing saponin waste water) is that 1:50 gets Staphylococcus saprophyticus (Staphylococcus saprophyticus) V430 and is inoculated in and contains in the high-concentration chlorine ion wastewater (as mixing saponin waste water), 25 ℃ of constant temperature anaerobism are cultivated, the pH value is 7.0, reacts 5 days.
Described enlarged culturing base is: peptone 10g; Extractum carnis 5g; Distilled water 1000ml; CaCl
218.0g/L, pH=7.5.
Application example 3:
The treatment process that one staphylococcus saprophyticus (Staphylococcus saprophyticus) V430 is applied to contain high-concentration chlorine ion wastewater is: the single bacterium colony of picking Staphylococcus saprophyticus (Staphylococcus saprophyticus) V430, in the enlarged culturing base, cultivated 24 hours, by Staphylococcus saprophyticus (Staphylococcus saprophyticus) V430 after cultivating: the volume ratio that contains high-concentration chlorine ion wastewater (as mixing saponin waste water) is that 4:50 gets Staphylococcus saprophyticus (Staphylococcus saprophyticus) V430 and is inoculated in and contains in the high-concentration chlorine ion wastewater (as mixing saponin waste water), 45 ℃ of constant temperature anaerobism are cultivated, the pH value is 8.0, reacts 3 days.
Described enlarged culturing base is: peptone 10g; Extractum carnis 5g; Distilled water 1000ml; CaCl
218.0g/L, pH=7.5.
＜110〉China Geological Univ. Wuhan
＜120〉application of anti-chlorine strain V 430
<160>1423
<210>1
<211>1423bp
<212>DNA
＜213〉Staphylococcus saprophyticus (Staphylococcus saprophyticus)
<220>
<221>misc_feature
<222>
<400>1
GTTCCATGTGGGTAACCTACCTATAAGACTGTTCTAACTCCGGGATACCGGGGCTAATGC 120
CGGATGGCATTAGAACCGGTTGCCCGGAGTGTGAAAGATGGTTTTGCTATCTCTTATACG 180
TGGACCCGCGCCGTATGAGGTAGTTGGTAAGGTAATGGCTTACCAAGGCAACGATACGTA 240
GCCGACCTGAGAGGGTGATCGGCCACACTGGAACTGAGACACGGTCCAACTCTCTACGGG 300
AGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGACGGAGCAACGCCGCGTGAG 360
TGATGAAGGGTTTCGGCTCGTAAAACTCTGTTATTAGGGAAGAACAAACGTGTAAGTAAC 420
TGTGCACGTCTTGACGGTACCTAATCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGC 480
GGTAATACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGTAGGCGG 540
TTTCTTAAGTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGA 600
GACTTGAGTGCAGAAGAGGAAAGTGGAATTCCATGTGTAGCGGTGAAATGCGCAGAGATA 660
TGGAGGAACACCAGTGGCGAAAGCGACTTTCTGGTCTGTAACTGACGCTGATGTGCGAAA 720
GCGTGGGGATCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTA 780
AGTGTTAGGGGGTTTCCGCCCCTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGG 840
GAGTACGACCGCAAGGTTGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAG 900
CATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAAATCTTGACATCCTTTGACCA 960
CTCTAGAGATAGAGTTTTCCCCTTCGGGGGACAAAGTGACAGGTGGTGCATGGTTGTCGT 1020
CAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTAAACTTAGT 1080
TGCCAGCATTTAGTTGGGCACTCTAGGTTGACTGCCGGTGACAAACCGGAGGAAGGTGGG 1140
TACAAAGGGCAGCTAAACCGCGAGGTCATGCAAATCCCATAAAGTTGTTCTCAGTTCGGA 1260
TTGTAGTCTGCAACTCGACTACATGAAGCTGGAATCGCTAGTAATCGTAGATCAGCATGC 1320
TACGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCACGAGAGTTTGTAA 1380
CACCCGAAGCCGGTGGAGTAACCATTAATGGAGCTAGCCGTCG 1423
Claims (3)
1. the application of a chlorine-resisting strain V430 is characterized in that a staphylococcus saprophyticus (Staphylococcus saprophyticus) the V430 CCTCC NO.M205145 that is applied as of described bacterial strain is applied to contain in the processing of high-concentration chlorine ion wastewater.
2. the application of a chlorine-resisting strain V430 according to claim 1, it is characterized in that: the treatment process that a described staphylococcus saprophyticus (Staphylococcus saprophyticus) V430 is applied to contain high-concentration chlorine ion wastewater is: the single bacterium colony of picking Staphylococcus saprophyticus (Staphylococcus saprophyticus) V430, in the enlarged culturing base, cultivated 20-24 hour, by Staphylococcus saprophyticus (Staphylococcus saprophyticus) V430 after cultivating: the volume ratio that contains high-concentration chlorine ion wastewater is (1-4): 50 get Staphylococcus saprophyticus (Staphylococcus saprophyticus) V430 is inoculated in and contains in the high-concentration chlorine ion wastewater, 25-45 ℃ of constant temperature anaerobism cultivated, the pH value is 7.0-8.0, reacts 5-7 days.
3. the application of a chlorine-resisting strain V430 according to claim 2 is characterized in that: described enlarged culturing base is: peptone 10g; Extractum carnis 5g; Distilled water 1000ml; CaCl
218.0g/L, pH=7.5.
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Non-Patent Citations (2)
| Title |
|---|
| 3株油脂化工废水降解菌的分离鉴定. 黄钧等.应用与环境生物学报，第5卷. 1999 |
| 3株油脂化工废水降解菌的分离鉴定. 黄钧等.应用与环境生物学报，第5卷. 1999 * |
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