CN100448976C - Method of preparing beer local flavor vinegar - Google Patents
Method of preparing beer local flavor vinegar Download PDFInfo
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- CN100448976C CN100448976C CNB2007100135333A CN200710013533A CN100448976C CN 100448976 C CN100448976 C CN 100448976C CN B2007100135333 A CNB2007100135333 A CN B2007100135333A CN 200710013533 A CN200710013533 A CN 200710013533A CN 100448976 C CN100448976 C CN 100448976C
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- beer
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Abstract
The invention discloses a preparing method of beer local flavor vinegar in brewing technical domain, which comprises the following steps: boiling waste beer; yeasting acetic acid mixed bacteria; clarifying; adding into clarificant; switching in scent yeast seed liquid in fermentation tank; proceeding flavor matter yeast; switching in acetic acid bacteria seed liquid; proceeding acetous fermentation; getting ripe fermented glutinour rice; adding into bentonite clay; stirring; stewing; depositing; fetching supernatant fluid; filtering with diatom earth filter; getting the clarifying beer local flavor vinegar. This invention resolves the problem of opacity and back-opacity, which can increase the scent composition of beer vinegar.
Description
Technical field
The present invention relates to a kind of preparation method of beer local flavor vinegar, belong to technology and brewing technology.
Background technology
In the brewing process of beer, can produce a large amount of yeast slurry by products, for 100,000 tons of production-scale beer enterprises of an annual output, its waste yeast mud can reach 2000 tons.Wherein more to the zymic development and use, mainly be used as feed, extract ATP, gsh, nucleic acid etc., and remaining waste beer is utilized not, all directly is discharged into and goes in the environment, has both wasted resource, has polluted environment again.These beer contain a large amount of yeast, and yeast autolysis discharged a large amount of indissoluble materials, are difficult to filtered and recycled, and we are referred to as " waste beer liquid " these beer.These " waste beer liquid " tastes are not good enough, are difficult to filter, and can influence the integral product quality even be blended in the finished beer after filtering yet.CN1201825 (98102602.8) discloses a kind of preparation technology who adopts beer yeast slurry to produce bright vinegar, comprises beer yeast slurry in the beer raffinate that washing precipitation obtains, and adds the acetate bacterial classification, acidifying is decomposed, and adds ferment-fermented then, sterilization adds flavor additives useful again, is mixed.The vinegar clarity of this method preparation is bad and exist and return muddy problem, and taste dullness, insufficient fragrance.It is the method that raw material is produced edible aromatic vinegar and acetic acid type beverage with beer inferior (being the beer that abnomal condition is produced) that CN1069765 (91105747.1) discloses a kind of, do not relate to clarifying problem equally, and its aromatic vinegar taste still adopts traditional allotment, with the method realization of flavour passage.
Summary of the invention
At the deficiencies in the prior art, the purpose of this invention is to provide a kind of preparation method of beer local flavor vinegar, utilize waste beer to produce clarifying beer vinegar, both avoided the wasting of resources, reduced environmental pollution again.
Another object of the present invention provides a kind of local flavor vinegar product of above-mentioned prepared.
Though the waste beer in the brewing process of beer can not be drunk as qualified beer, but still contain multiple nutritional components such as rich in protein, VITAMIN, mineral substance, alcohol, these nutritive ingredients are fit to acetic bacteria growth fermentation just, it is the most suitable substratum of acetic bacteria growth, with the waste beer is raw material, by aerobic fermentation the ethanol of about 4.0% (w/w) in the waste beer is converted into acetic acid through acetic fermentation, produces alegar, both avoided the wasting of resources, and had and reduced environmental pollution.
Technical scheme of the present invention is as follows:
A kind of preparation method of beer local flavor vinegar comprises that waste beer liquid boils, the acetic acid mixed fungus fermentation, and clarifying treatment, concrete steps are as follows:
1. waste beer liquid boils
Beer yeast slurry is centrifugal, obtains supernatant liquor and boils under 0.05～0.15Mpa pressure in boiling pot, boil beginning 10～15min after, add finings, addition is 0.005%～0.015% mass percent, boiling time is 20～70 minutes.Staticly settle then; Remove precipitation, liquid is standby.Beer yeast slurry centrifugal rotational speed 2000-5000r/min.
2. acetic fermentation
Waste beer liquid after step 1 is handled is cooled to 28～35 ℃, moves into fermentor tank.At first insert aroma-producing yeast seed liquor 1%～5% (volume percent), carry out the fragrance matter fermentation, after 20～45 hours, insert the acetic bacteria seed liquid of 6%～10% (volume percent), carry out acetic fermentation.The liquid amount of fermentor tank is 60%～80%, and leavening temperature is 28～35 ℃, and ventilating ratio is 1: 0.1～1: 0.3 (volume ratio), and tank pressure is 0.01～0.05Mpa, ferments to alcohol residual volume 0.1%～0.5%, stops fermentation.
3. clarifying treatment
In the karusen of step 2 fermenting-ripening, add the bentonite of 0.05%～0.3% (mass percent) or the mixture of bentonite and polyvinylpyrrolidone, stirred 1～2 hour, staticly settled then 4～6 hours, and got supernatant liquor and utilize diatomite filter to filter, get clarifying beer local flavor vinegar.
The preferred red promise B of finings in the above-mentioned steps 1.Belgium aginomoto Ou Mingke company produces.
After boiling end in the above-mentioned steps 1, waste beer liquid enters settling bath with tangential direction, staticly settles 20～40 minutes, gets supernatant liquor, as the acetic fermentation substratum.
The preferred geotrichum candidum of the aroma-producing yeast of above-mentioned steps 2.
Aroma-producing yeast seed liquor in the above-mentioned steps 2 and acetic bacteria seed liquid all can be by prior art for preparing.The invention provides the following bacterial classification method that spreads cultivation;
A. acetic acid bacteria strain spreads cultivation
(1) first order seed: acetic bacteria seed substratum is placed the 250ml triangular flask, 121 ℃ of sterilization 15min, be cooled to 70 ℃, the dehydrated alcohol that adds 2%-5% (volume percent) inserts AS1.41 acetic bacteria slant culture bacterial classification, inoculum size 3-5%, 28-32 ℃ of shaking table cultivated, used the sodium hydroxide titration in per 12 hours, acidity rises to 1.5%-2%, gets first order seed.
(2) beer liquid that will boil after-filtration places the 10L fermentor tank, and liquid amount is 60%-80%, and sterilization 15min is cooled to 28-32 ℃, inserts the first order seed of 10% (volume percent), 30 ℃ of ventilating fermentations, to acidity 1.5%-2%, secondary seed.Same quadrat method, spread cultivation three grades of seeds.
B. aroma-producing yeast spreads cultivation
Respectively at packing 100mL wheat juice in the 250mL triangular flask, 121 ℃ of sterilization 15min insert the aroma-producing yeast slant strains, inoculum size 3-5%, 28 ℃ of shaking tables are cultivated, and cultivate 30h, get first order seed, spread cultivation successively, to secondary, three grades of seeds are the aroma-producing yeast seed liquor, and are stand-by.
Technical characterstic of the present invention and excellent results are as follows:
1, utilizes waste beer to produce clarifying beer vinegar, both made full use of grain resource, reduced environmental pollution again.
2, the present invention adopts cost-effective method, has solved the muddy problem of returning of alegar.At first, can adsorb high molecular weight protein, alegar has been played clarification by boiling, add finings.Secondly, after fermentation, add bentonite in the acetic fermentation wine with dregs, strengthened filter effect, increased filtration efficiency.Further solve alegar muddiness, return muddy problem.
3, the realization of flavour ingredient: liquid state fermentation vinegar exists flavour ingredient single, the shortcoming of taste dullness, and the present invention has increased the flavour ingredient of alegar by inserting aroma-producing yeast and acetic bacteria mixed fermentation, has improved the alegar taste by fermentation process.
Embodiment
Embodiment 1: bacterial classification spreads cultivation
A. acetic acid bacteria strain spreads cultivation
Seed culture medium: glucose 10g, yeast extract paste 10g, KH
2PO
43g, MgSO
42g, dehydrated alcohol 40ml (the sterilization back adds), 1000 milliliters of distilled water.
First order seed: acetic bacteria seed substratum is placed the 250ml triangular flask, 121 ℃ of sterilization 15min, be cooled to 70 ℃, the dehydrated alcohol that adds 4% (volume percent), insert AS1.41 acetic bacteria slant culture bacterial classification, 30 ℃ of shaking tables are cultivated, and use the sodium hydroxide titration in per 12 hours, acidity rises to 2%, gets first order seed.
Secondary seed: the beer liquid that will boil after-filtration places the 10L fermentor tank, and dress liquid coefficient is 70%, sterilization 15min, and cooling, the first order seed of access 10% (volume percent), 30 ℃ of ventilating fermentations to acidity 2%, get secondary seed.Same quadrat method, spread cultivation three grades of seeds of acetic bacteria.
B. the aroma-producing yeast geotrichum candidum spreads cultivation
Respectively at packing 100mL wheat juice in the 250mL triangular flask, 121 ℃ of sterilization 15min insert aroma-producing yeast geotrichum candidum slant strains, and 28 ℃ of shaking tables are cultivated, and cultivate 30h, get first order seed.Spreading cultivation successively,, stand-by to secondary, three grades of seeds by 10% (volume percent) inoculation.
Embodiment 2: the preparation of beer local flavor vinegar
1, beer yeast slurry is centrifugal, rotating speed 4000r/min gets supernatant liquor, boils, and adds the red promise B of finings (Belgian aginomoto Ou Mingke company produce) 0.01% (mass percent), adds boiling beginning back 10min.Under 0.08Mpa pressure, boil 30min, boil end after, waste beer liquid enters settling bath with tangential direction, staticly settles 20～40 minutes, gets supernatant liquor, as the acetic fermentation substratum.
2, fermentation
Waste beer liquid after boiling moves into self-priming acetic fermentation jar after cooling off, at first insert 5% (volume percent) aroma-producing yeast geotrichum candidum seed liquor (embodiment 1) to increase the fragrance of alegar, ferment after 24 hours, insert three grades of seeds of acetic bacteria (embodiment 1) of 10% (volume percent), carry out acetic fermentation, liquid amount is 70%, 30-32 ℃ of control leavening temperature, ventilating fermentation, control suitable ventilating ratio, the volume ratio of air flow and fermented liq is 1: 0.15, tank pressure 0.02Mpa, and fermentation 56h is to alcohol 0.2% (volume percent) fermentation termination.
3, the filtration of fermentation liquid
Add bentonite in the acetic fermentation wine with dregs that fermentation finishes,, alegar is clarified with residual macro-molecular protein in the absorption mash.In the karusen after acetic fermentation finishes, add the bentonite of 0.1%wt, stirred 1 hour, staticly settled then 4 hours.Utilize diatomite filter to filter then, get clarifying beer local flavor vinegar.
Embodiment 3: as described in embodiment 2, different is
1, with embodiment 1
2, fermentation
Waste beer liquid after boiling moves into self-priming acetic fermentation jar after cooling off, insert 3% (volume percent) aroma-producing yeast geotrichum candidum seed liquor and carry out the fragrance matter fermentation, ferment after 30 hours, insert three grades of seeds of acetic bacteria of 8% (volume percent), carry out acetic fermentation, liquid amount is 80%, 30 ℃ of control leavening temperatures, ventilating fermentation, control suitable ventilating ratio, the volume ratio of air flow and fermented liq is 1: 0.15, tank pressure 0.03Mpa, and fermentation 56h is to alcohol 0.2% (volume percent) fermentation termination.
3, the filtration of fermentation liquid
The mixture (bentonite and PVPP weight ratio are 2: 1) that adds bentonite and polyvinylpyrrolidone (PVPP) in the acetic fermentation wine with dregs that fermentation finishes, the total amount that adds the mixture of bentonite and polyvinylpyrrolidone is the 0.15%wt of fermentation liquid, stirred 2 hours, staticly settled then 5 hours.Utilize diatomite filter to filter then, get clarifying beer local flavor vinegar.
Claims (5)
1, a kind of preparation method of beer local flavor vinegar comprises that waste beer liquid boils, the acetic acid mixed fungus fermentation, and clarifying treatment, concrete steps are as follows:
(1) beer yeast slurry is centrifugal, obtains supernatant liquor and boils under 0.05～0.15Mpa pressure in boiling pot, boil beginning 10～15min after, add finings, addition is 0.005%～0.015% mass percent, boiling time is 20～70 minutes; Staticly settle then; Remove precipitation, liquid is standby;
(2) the waste beer liquid after step (1) is handled is cooled to 28～35 ℃, moves into fermentor tank; At first insert geotrichum candidum seed liquor 1%～5% volume percent, carry out the fragrance matter fermentation, after 20～45 hours, insert the acetic bacteria seed liquid of 6%～10% volume percent, carry out acetic fermentation; The liquid amount of fermentor tank is 60%～80%, and leavening temperature is 28～35 ℃, and ventilating ratio is 1: 0.1～1: 0.3 volume ratio, and tank pressure is 0.01～0.05Mpa, ferments to alcohol residual volume 0.1%～0.5%, stops fermentation;
(3) in the karusen of step (2) fermenting-ripening, add the bentonite of 0.05%～0.3% mass percent or the mixture of bentonite and polyvinylpyrrolidone, stirred 1～2 hour, staticly settled then 4～6 hours, get supernatant liquor and utilize diatomite filter to filter, get clarifying beer local flavor vinegar.
2, the preparation method of beer local flavor vinegar as claimed in claim 1 is characterized in that the finings in the described step (1) is red promise B.
3, the preparation method of beer local flavor vinegar as claimed in claim 1, after it is characterized in that boiling end in the described step (1), waste beer liquid enters settling bath with tangential direction, staticly settles 20～40 minutes, gets supernatant liquor, as the acetic fermentation substratum.
4, the preparation method of beer local flavor vinegar as claimed in claim 1 is characterized in that in the described step (2) that the geotrichum candidum seed liquor bacterial classification method that spreads cultivation is as follows:
Respectively at packing 100mL wheat juice in the 250mL triangular flask, 121 ℃ of sterilization 15min insert the geotrichum candidum slant strains, inoculum size 3-5%, and 28 ℃ of shaking tables are cultivated, and cultivate 30h, get first order seed, spread cultivation successively, and to secondary, three grades of seeds promptly get the geotrichum candidum seed liquor.
5, the preparation method of beer local flavor vinegar as claimed in claim 1 is characterized in that in the described step (2) that the acetic bacteria seed fluid strain method that spreads cultivation is as follows:
(1) acetic bacteria seed substratum is placed the 250ml triangular flask, 121 ℃ of sterilization 15min, be cooled to 70 ℃, the dehydrated alcohol that adds the 2%-5% volume percent inserts AS1.41 acetic bacteria slant culture bacterial classification, inoculum size 3-5%, 28-32 ℃ of shaking table cultivated, used the sodium hydroxide titration in per 12 hours, acidity rises to 1.5%-2%, gets first order seed;
(2) beer liquid that will boil after-filtration places the 10L fermentor tank, and liquid amount is 60%-80%, and sterilization 15min is cooled to 28-32 ℃, inserts the first order seed of 10% volume percent, 30 ℃ of ventilating fermentations, to acidity 1.5%-2%, secondary seed; Same quadrat method, spread cultivation three grades of seeds.
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2007100135333A CN100448976C (en) | 2007-02-09 | 2007-02-09 | Method of preparing beer local flavor vinegar |
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|---|---|---|---|
| CNB2007100135333A CN100448976C (en) | 2007-02-09 | 2007-02-09 | Method of preparing beer local flavor vinegar |
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| CN101016509A CN101016509A (en) | 2007-08-15 |
| CN100448976C true CN100448976C (en) | 2009-01-07 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103320302A (en) * | 2013-05-27 | 2013-09-25 | 灵武市果业开发有限责任公司 | Multi-strain fermentation technology of red date vinegar |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101993800B (en) * | 2009-08-24 | 2013-04-03 | 广州珠江啤酒股份有限公司 | Method for producing turbid beer or turbid white beer |
| CN103695286B (en) * | 2014-01-03 | 2014-12-03 | 南京飞马食品有限公司 | Method for producing beer vinegar |
| CN105802827B (en) * | 2016-05-30 | 2018-10-26 | 山西大学 | A method of producing Hawthorn Vinegar using hawthorn liquid glucose |
| CN106350430A (en) * | 2016-09-14 | 2017-01-25 | 元壮河北生物科技有限公司 | Beer vinegar and preparation method thereof |
| RU2663002C1 (en) * | 2017-12-21 | 2018-07-31 | Федеральное государственное бюджетное научное учреждение "Федеральный научный центр пищевых систем им. В.М. Горбатова" РАН | Method for producing food vinegar |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1069765A (en) * | 1991-08-16 | 1993-03-10 | 辽宁师范大学 | Waste beer is produced the method for edible aromatic vinegar and acetic acid type beverage |
| CN1201825A (en) * | 1998-06-16 | 1998-12-16 | 王金鸣 | Process for producing vineger by using beer yeast mud and product thereby |
-
2007
- 2007-02-09 CN CNB2007100135333A patent/CN100448976C/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1069765A (en) * | 1991-08-16 | 1993-03-10 | 辽宁师范大学 | Waste beer is produced the method for edible aromatic vinegar and acetic acid type beverage |
| CN1201825A (en) * | 1998-06-16 | 1998-12-16 | 王金鸣 | Process for producing vineger by using beer yeast mud and product thereby |
Non-Patent Citations (2)
| Title |
|---|
| 利用酒头、酒尾研制啤酒营养白醋. 朱奇，石海英，陈彦等.酿酒科技，第1期. 2004 |
| 利用酒头、酒尾研制啤酒营养白醋. 朱奇，石海英，陈彦等.酿酒科技，第1期. 2004 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103320302A (en) * | 2013-05-27 | 2013-09-25 | 灵武市果业开发有限责任公司 | Multi-strain fermentation technology of red date vinegar |
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