CN100448476C - Quality control method of Xiaoer Xishi Tablet for improving children appetite - Google Patents
Quality control method of Xiaoer Xishi Tablet for improving children appetite Download PDFInfo
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- CN100448476C CN100448476C CNB2003101115625A CN200310111562A CN100448476C CN 100448476 C CN100448476 C CN 100448476C CN B2003101115625 A CNB2003101115625 A CN B2003101115625A CN 200310111562 A CN200310111562 A CN 200310111562A CN 100448476 C CN100448476 C CN 100448476C
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- 238000003908 quality control method Methods 0.000 title claims description 20
- 230000036528 appetite Effects 0.000 title abstract 4
- 235000019789 appetite Nutrition 0.000 title abstract 4
- 239000000463 material Substances 0.000 claims abstract description 41
- WCGUUGGRBIKTOS-GPOJBZKASA-N (3beta)-3-hydroxyurs-12-en-28-oic acid Chemical compound C1C[C@H](O)C(C)(C)[C@@H]2CC[C@@]3(C)[C@]4(C)CC[C@@]5(C(O)=O)CC[C@@H](C)[C@H](C)[C@H]5C4=CC[C@@H]3[C@]21C WCGUUGGRBIKTOS-GPOJBZKASA-N 0.000 claims abstract description 32
- 229940096998 ursolic acid Drugs 0.000 claims abstract description 32
- PLSAJKYPRJGMHO-UHFFFAOYSA-N ursolic acid Natural products CC1CCC2(CCC3(C)C(C=CC4C5(C)CCC(O)C(C)(C)C5CCC34C)C2C1C)C(=O)O PLSAJKYPRJGMHO-UHFFFAOYSA-N 0.000 claims abstract description 32
- 238000000034 method Methods 0.000 claims abstract description 23
- 238000004809 thin layer chromatography Methods 0.000 claims abstract description 19
- 238000001514 detection method Methods 0.000 claims abstract description 12
- 238000004811 liquid chromatography Methods 0.000 claims abstract description 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 81
- 238000012360 testing method Methods 0.000 claims description 57
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 54
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- 239000003814 drug Substances 0.000 claims description 44
- 238000002360 preparation method Methods 0.000 claims description 42
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 34
- 229940079593 drug Drugs 0.000 claims description 26
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 24
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 24
- 239000003208 petroleum Substances 0.000 claims description 24
- 239000013558 reference substance Substances 0.000 claims description 24
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 claims description 18
- 239000000047 product Substances 0.000 claims description 17
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 16
- 238000000605 extraction Methods 0.000 claims description 16
- 239000000706 filtrate Substances 0.000 claims description 16
- 239000007788 liquid Substances 0.000 claims description 14
- 238000002156 mixing Methods 0.000 claims description 12
- 238000005303 weighing Methods 0.000 claims description 10
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims description 8
- 201000006549 dyspepsia Diseases 0.000 claims description 8
- KXUYYFNHOZRVKP-UHFFFAOYSA-N ethanol;4-hydroxy-3-methoxybenzaldehyde Chemical compound CCO.COC1=CC(C=O)=CC=C1O KXUYYFNHOZRVKP-UHFFFAOYSA-N 0.000 claims description 8
- 239000000945 filler Substances 0.000 claims description 8
- YTJSFYQNRXLOIC-UHFFFAOYSA-N octadecylsilane Chemical compound CCCCCCCCCCCCCCCCCC[SiH3] YTJSFYQNRXLOIC-UHFFFAOYSA-N 0.000 claims description 8
- 238000010992 reflux Methods 0.000 claims description 8
- 239000000741 silica gel Substances 0.000 claims description 8
- 229910002027 silica gel Inorganic materials 0.000 claims description 8
- 239000000377 silicon dioxide Substances 0.000 claims description 8
- 239000002904 solvent Substances 0.000 claims description 8
- 239000007921 spray Substances 0.000 claims description 8
- 239000001117 sulphuric acid Substances 0.000 claims description 8
- 235000011149 sulphuric acid Nutrition 0.000 claims description 8
- MWOOGOJBHIARFG-UHFFFAOYSA-N vanillin Chemical compound COC1=CC(C=O)=CC=C1O MWOOGOJBHIARFG-UHFFFAOYSA-N 0.000 claims description 8
- FGQOOHJZONJGDT-UHFFFAOYSA-N vanillin Natural products COC1=CC(O)=CC(C=O)=C1 FGQOOHJZONJGDT-UHFFFAOYSA-N 0.000 claims description 8
- 235000012141 vanillin Nutrition 0.000 claims description 8
- 230000004580 weight loss Effects 0.000 claims description 8
- 239000010271 massa medicata fermentata Substances 0.000 claims description 7
- 230000005180 public health Effects 0.000 claims description 6
- 239000002994 raw material Substances 0.000 claims description 6
- 238000009472 formulation Methods 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
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- 238000003556 assay Methods 0.000 claims description 3
- SRCZQMGIVIYBBJ-UHFFFAOYSA-N ethoxyethane;ethyl acetate Chemical compound CCOCC.CCOC(C)=O SRCZQMGIVIYBBJ-UHFFFAOYSA-N 0.000 claims 2
- 230000001737 promoting effect Effects 0.000 abstract description 5
- 241000132012 Atractylodes Species 0.000 abstract 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 7
- 238000005516 engineering process Methods 0.000 description 6
- 239000000796 flavoring agent Substances 0.000 description 5
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- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 239000008187 granular material Substances 0.000 description 4
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- 239000000843 powder Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 210000002784 stomach Anatomy 0.000 description 3
- 235000017159 Crataegus pinnatifida Nutrition 0.000 description 2
- 241000657480 Crataegus pinnatifida Species 0.000 description 2
- PBCJIPOGFJYBJE-UHFFFAOYSA-N acetonitrile;hydrate Chemical compound O.CC#N PBCJIPOGFJYBJE-UHFFFAOYSA-N 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- PFTAWBLQPZVEMU-ZFWWWQNUSA-N (+)-epicatechin Natural products C1([C@@H]2OC3=CC(O)=CC(O)=C3C[C@@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-ZFWWWQNUSA-N 0.000 description 1
- PFTAWBLQPZVEMU-UKRRQHHQSA-N (-)-epicatechin Chemical compound C1([C@H]2OC3=CC(O)=CC(O)=C3C[C@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-UKRRQHHQSA-N 0.000 description 1
- 241000092665 Atractylodes macrocephala Species 0.000 description 1
- 235000008495 Chrysanthemum leucanthemum Nutrition 0.000 description 1
- 244000192528 Chrysanthemum parthenium Species 0.000 description 1
- 235000000604 Chrysanthemum parthenium Nutrition 0.000 description 1
- 244000183685 Citrus aurantium Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- OVSQVDMCBVZWGM-SJWGPRHPSA-N Hyperin Natural products O[C@H]1[C@H](O)[C@@H](O)[C@@H](CO)O[C@H]1OC1=C(C=2C=C(O)C(O)=CC=2)OC2=CC(O)=CC(O)=C2C1=O OVSQVDMCBVZWGM-SJWGPRHPSA-N 0.000 description 1
- FVQOMEDMFUMIMO-UHFFFAOYSA-N Hyperosid Natural products OC1C(O)C(O)C(CO)OC1OC1C(=O)C2=C(O)C=C(O)C=C2OC1C1=CC=C(O)C(O)=C1 FVQOMEDMFUMIMO-UHFFFAOYSA-N 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- MDZKJHQSJHYOHJ-UHFFFAOYSA-N crataegolic acid Natural products C1C(O)C(O)C(C)(C)C2CCC3(C)C4(C)CCC5(C(O)=O)CCC(C)(C)CC5C4=CCC3C21C MDZKJHQSJHYOHJ-UHFFFAOYSA-N 0.000 description 1
- 206010061428 decreased appetite Diseases 0.000 description 1
- 230000000741 diarrhetic effect Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 239000002934 diuretic Substances 0.000 description 1
- 230000001882 diuretic effect Effects 0.000 description 1
- LPTRNLNOHUVQMS-UHFFFAOYSA-N epicatechin Natural products Cc1cc(O)cc2OC(C(O)Cc12)c1ccc(O)c(O)c1 LPTRNLNOHUVQMS-UHFFFAOYSA-N 0.000 description 1
- 235000012734 epicatechin Nutrition 0.000 description 1
- 235000008384 feverfew Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- MDZKJHQSJHYOHJ-LLICELPBSA-N maslinic acid Chemical compound C1[C@@H](O)[C@H](O)C(C)(C)[C@@H]2CC[C@@]3(C)[C@]4(C)CC[C@@]5(C(O)=O)CCC(C)(C)C[C@H]5C4=CC[C@@H]3[C@]21C MDZKJHQSJHYOHJ-LLICELPBSA-N 0.000 description 1
- 239000012567 medical material Substances 0.000 description 1
- OJURWUUOVGOHJZ-UHFFFAOYSA-N methyl 2-[(2-acetyloxyphenyl)methyl-[2-[(2-acetyloxyphenyl)methyl-(2-methoxy-2-oxoethyl)amino]ethyl]amino]acetate Chemical compound C=1C=CC=C(OC(C)=O)C=1CN(CC(=O)OC)CCN(CC(=O)OC)CC1=CC=CC=C1OC(C)=O OJURWUUOVGOHJZ-UHFFFAOYSA-N 0.000 description 1
- 150000004702 methyl esters Chemical class 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 239000008194 pharmaceutical composition Substances 0.000 description 1
- OVSQVDMCBVZWGM-DTGCRPNFSA-N quercetin 3-O-beta-D-galactopyranoside Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1OC1=C(C=2C=C(O)C(O)=CC=2)OC2=CC(O)=CC(O)=C2C1=O OVSQVDMCBVZWGM-DTGCRPNFSA-N 0.000 description 1
- BBFYUPYFXSSMNV-UHFFFAOYSA-N quercetin-7-o-galactoside Natural products OC1C(O)C(O)C(CO)OC1OC1=CC(O)=C2C(=O)C(O)=C(C=3C=C(O)C(O)=CC=3)OC2=C1 BBFYUPYFXSSMNV-UHFFFAOYSA-N 0.000 description 1
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- 239000000126 substance Substances 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- -1 trimethyl Chemical compound 0.000 description 1
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- Medicines Containing Plant Substances (AREA)
- Medicinal Preparation (AREA)
Abstract
The present invention relates to a method for controlling the quality of appetite promoting tablets for children, which is characterized in that the ursolic acid content of the appetite promoting tablets for children is determined by high efficiency liquid chromatography, and white atractylodes rhizome which is Chinese medicinal material is discriminated by thin layer chromatography. The method ensures the precision and the advancement of quality detection standards so as to effectively control the quality of the appetite promoting tablets for children.
Description
Technical field
The present invention relates to the method for quality control of treatment pediatric disease medicine among second WS3-B-0197-90 of a kind of " Drug Standard of Ministry of Public Health of the Peoples Republic of China " (Chinese traditional patent formulation preparation), be specifically related to a kind of method of quality control of the treatment pediatric disease medicine of making by Chinese crude drug Massa Medicata Fermentata, Fructus Aurantii, the Rhizoma Atractylodis Macrocephalae, Fructus Crataegi, Fructus Oryzae Germinatus, Fructus Hordei Germinatus, particularly relate to the treatment infantile simple dyspepsia, the diarrheal XIAOER XISHI PIAN method of quality control that inappetence and dyspepsia cause.
Background technology
Treatment pediatric disease medicine XIAOER XISHI PIAN among second WS3-B-0197-90 of a kind of " Drug Standard of Ministry of Public Health of the Peoples Republic of China " (Chinese traditional patent formulation preparation), this pharmaceutical formulation is made up of Chinese crude drug Massa Medicata Fermentata (stir-fry) 77g, Fructus Aurantii (parched) 39g, the Rhizoma Atractylodis Macrocephalae (stir-fry) 39g, Fructus Crataegi 59g, Fructus Oryzae Germinatus (stir-fry) 192g, Fructus Hordei Germinatus (stir-fry) 192g, and above-mentioned raw materials is made 1000 altogether; Main flavour of a drug in the Fructus Crataegi side of being, dry mature fruit for rosaceous plant Fructus Pyri Pashiae Crataegus pinnatifida Bge.Var.majorN.E.Br. or Fructus Crataegi Crataegus pinnatifida Bge., has promoting digestion and invigorating the stomach, the effect of circulation of qi promoting dissipating blood stasis mainly contains chemical constituents such as Crataegolic acid, epicatechin, hyperin, citric acid and methyl ester, dimethyl ester, trimethyl, ursolic acid; The Rhizoma Atractylodis Macrocephalae is the dry rhizome of feverfew Rhizoma Atractylodis Macrocephalae Atractylodes macrocephala Koidz., has the effect of invigorating the spleen and benefiting QI, dampness diuretic; Fructus Aurantii is rutaceae Citrus aurantium Linn. and the dry last mature fruit of carrying the training mutation thereof, and bitter in the mouth, suffering, acid, temperature have regulating the flow of QI to ease the stomach, and the stagnant relieving distension of row is used for the breast side of body stagnation of QI, distension pain, the effect of accumulation of food in the stomach and intes tine due to indigestion.Second WS3-B-0197-90 of " Drug Standard of Ministry of Public Health of the Peoples Republic of China " (Chinese traditional patent formulation preparation) issued the XIAOER XISHI PIAN quality control standard, but in the existing XIAOER XISHI PIAN method of quality control that Ministry of Public Health is issued, has only microscopical identification, limitation is very big, the quality of restive XIAOER XISHI PIAN; The present invention overcomes the deficiencies in the prior art, improve the quality control standard of XIAOER XISHI PIAN, set up assay index and detection method thereof in the preparation, increase the wherein thin layer chromatography qualitative identification method of Rhizoma Atractylodis Macrocephalae medical material, guaranteed this compound preparation higher quality standard level.
Summary of the invention
The objective of the invention is to overcome deficiency of the prior art, formulate new XIAOER XISHI PIAN method of quality control, utilize content of ursolic acid in this medicine of high effective liquid chromatography for measuring; Differentiate this medicine Chinese crude drug Rhizoma Atractylodis Macrocephalae with thin layer chromatography, guaranteed the accuracy and the advance of quality inspection standard, can control the quality of XIAOER XISHI PIAN effectively.
The present invention is achieved by following technical proposals:
The quality control detection method of XIAOER XISHI PIAN, this method can be undertaken by following determination step:
1, measuring content of ursolic acid with high performance liquid chromatography (appendix VID of Chinese Pharmacopoeia version in 2000) measures and standard:
A. chromatographic condition: with octadecylsilane chemically bonded silica is filler; (70-90: 30-10) be mobile phase, the detection wavelength is 210 ± 2nm to methanol 0.1% phosphoric acid solution.
B. the preparation of reference substance solution: precision is got and is claimed that the ursolic acid reference substance is an amount of, adds in methanol or ethanol or the acetonitrile any and makes the solution that every 1ml contains 6--60 μ g;
C. the preparation of need testing solution: get the content under this product content uniformity item, mixing, porphyrize, get 0.5-6g, the accurate title, decide, and accurate any 25-100ml that adds in methanol or ethanol or the acetonitrile claims to decide weight, reflux 1 hour, put coldly, claim to decide weight again, supply with methanol or ethanol or acetonitrile and subtract weight loss, filter, promptly.
D. algoscopy: precision is measured reference substance liquid and each 5-20 μ l of need testing solution respectively, injects chromatograph of liquid, measures, promptly.Every (heavy 0.3g) XIAOER XISHI PIAN contains Fructus Crataegi in ursolic acid, must not be less than 0.10mg.
2. differentiate this medicine Chinese crude drug Rhizoma Atractylodis Macrocephalae with thin layer chromatography:
A. the preparation of control medicinal material solution: take by weighing Rhizoma Atractylodis Macrocephalae control medicinal material 0.25-1g, add petroleum ether (60-90 ℃) 20-100ml, supersound extraction 15-60min filters, and filtrate evaporate to dryness, residue add ethyl acetate 0.5ml makes dissolving, makes control medicinal material solution.
B. the preparation of need testing solution: get this product 8-28 sheet, every heavy 0.3 gram grinds, and adds petroleum ether (60-90 ℃) 20-100ml, and supersound extraction 15-60min filters, and filtrate evaporate to dryness, residue add ethyl acetate 0.5ml makes dissolving, as need testing solution.
C. test according to thin layer chromatography (appendix VIB of Chinese Pharmacopoeia version in 2000), get above-mentioned Rhizoma Atractylodis Macrocephalae control medicinal material solution 5-20 μ l, need testing solution 5-20 μ l, put respectively on same silica gel g thin-layer plate, with petroleum ether (60-90 ℃)-ethyl acetate (50: 1) is developing solvent, launch, take out, dry, spray (is got vanillin 1g and sulphuric acid 10ml with the vanillin ethanol solution of sulfuric acid, add ethanol to 100ml), in 105 ℃ of bakings about 5-15 minute, in the test sample chromatograph, with control medicinal material solution chromatograph relevant position on, show the speckle of same color.
Optimized technical scheme of the present invention can be:
The quality control detection method that XIAOER XISHI PIAN is new, this method also can be undertaken by following determination step:
1, measuring content of ursolic acid with high performance liquid chromatography (appendix VID of Chinese Pharmacopoeia version in 2000) measures and standard:
A. chromatographic condition: with octadecylsilane chemically bonded silica is filler; Methanol 0.1% phosphoric acid solution (87: 13) is a mobile phase, and the detection wavelength is 210nm.
B. the preparation of reference substance solution: precision is got and is claimed that the ursolic acid reference substance is an amount of, adds methanol and makes the solution that every 1ml contains 25 μ g, promptly.
C. the preparation of need testing solution: get the content under this product content uniformity item, mixing, porphyrize is got 1.5g, accurate claims surely, and the accurate methanol 25ml that adds claims decide weight, and reflux 1 hour is put coldly, claims to decide weight again, supply with methanol to subtract weight loss, filtration, promptly.
D. algoscopy: precision is measured reference substance liquid and each 20 μ l of need testing solution respectively, injects chromatograph of liquid, measures, promptly.Every (heavy 0.3g) XIAOER XISHI PIAN contains Fructus Crataegi in ursolic acid, must not be less than 0.10mg.
2. differentiate this medicine Chinese crude drug Rhizoma Atractylodis Macrocephalae with thin layer chromatography:
A. the preparation of control medicinal material solution: take by weighing Rhizoma Atractylodis Macrocephalae control medicinal material 0.5g, add petroleum ether (60-90 ℃) 40ml, supersound extraction 30min filters, and filtrate evaporate to dryness, residue add ethyl acetate 0.5ml makes dissolving, makes control medicinal material solution.
B. the preparation of need testing solution: get 18 of this product, every heavy 0.3 gram grinds, and adds petroleum ether (60-90 ℃) 40ml, and supersound extraction 30min filters, and filtrate evaporate to dryness, residue add ethyl acetate 0.5ml makes dissolving, as need testing solution.
C. test according to thin layer chromatography (appendix VIB of Chinese Pharmacopoeia version in 2000), get above-mentioned Rhizoma Atractylodis Macrocephalae control medicinal material solution 10 μ l, need testing solution 15 μ l, put respectively on same silica gel g thin-layer plate, with petroleum ether (60-90 ℃)-ethyl acetate (50: 1) is developing solvent, launch, take out, dry, spray (is got vanillin 1g and sulphuric acid 10ml with the vanillin ethanol solution of sulfuric acid, add ethanol to 100ml), in about 10 minutes of 105 ℃ of bakings, in the test sample chromatograph, with control medicinal material solution chromatograph relevant position on, show the speckle of same color.
The preparation method of treatment pediatric disease medicine XIAOER XISHI PIAN of the present invention is to make every sheet weight 0.3g by the preparation method among second WS3-B-0197-90 of " Drug Standard of Ministry of Public Health of the Peoples Republic of China " (Chinese traditional patent formulation preparation).
The TCL that the invention provides the Rhizoma Atractylodis Macrocephalae differentiates that having selected the higher ursolic acid of content is testing index, has set up the HPLC content assaying method of XIAOER XISHI PIAN; Through repeated trials repeatedly, confirmation method is easy, the result is accurate, can be used as the XIAOER XISHI PIAN quality control and investigates the index of the stability of technology.
The specific embodiment
Embodiment 1: get Chinese crude drug Massa Medicata Fermentata (stir-fry) 77g, Fructus Aurantii (parched) 39g, the Rhizoma Atractylodis Macrocephalae (stir-fry) 39g, Fructus Crataegi 59g, Fructus Oryzae Germinatus (stir-fry) 192g, Fructus Hordei Germinatus (stir-fry) 192g, above-mentioned raw materials Fructus Oryzae Germinatus, Fructus Hordei Germinatus decoct with water 2 times, and each 30 minutes, collecting decoction, filter, be condensed into the thick paste shape, all the other four flavors are ground into fine powder, with above-mentioned thick paste mixing, add refined honey 115g, make granule, be pressed into 1000, every sheet weight 0.3g:
1, measuring content of ursolic acid with high performance liquid chromatography (appendix VID of Chinese Pharmacopoeia version in 2000) measures and standard:
A. chromatographic condition: with octadecylsilane chemically bonded silica is filler; Methanol 0.1% phosphoric acid solution (87: 13) is a mobile phase, and the detection wavelength is 210nm.
B. the preparation of reference substance solution: precision is got and is claimed that the ursolic acid reference substance is an amount of, adds methanol and makes the solution that every 1ml contains 25 μ g, promptly.
C. the preparation of need testing solution: get the content under this product content uniformity item, mixing, porphyrize is got 1.5g, accurate claims surely, and the accurate methanol 25ml that adds claims decide weight, and reflux 1 hour is put coldly, claims to decide weight again, supply with methanol to subtract weight loss, filtration, promptly.
D. algoscopy: precision is measured reference substance liquid and each 20 μ l of need testing solution respectively, injects chromatograph of liquid, measures, promptly.Every (heavy 0.3g) XIAOER XISHI PIAN contains Fructus Crataegi in ursolic acid, must not be less than 0.10mg.
2. differentiate this medicine Chinese crude drug Rhizoma Atractylodis Macrocephalae with thin layer chromatography:
A. the preparation of control medicinal material solution: take by weighing Rhizoma Atractylodis Macrocephalae control medicinal material 0.5g, add petroleum ether (60-90 ℃) 40ml, supersound extraction 30min filters, and filtrate evaporate to dryness, residue add ethyl acetate 0.5ml makes dissolving, makes control medicinal material solution.
B. the preparation of need testing solution: get 18 of this product, every heavy 0.3 gram grinds, and adds petroleum ether (60-90 ℃) 40ml, and supersound extraction 30min filters, and filtrate evaporate to dryness, residue add ethyl acetate 0.5ml makes dissolving, as need testing solution.
C. test according to thin layer chromatography (appendix VIB of Chinese Pharmacopoeia version in 2000), get above-mentioned Rhizoma Atractylodis Macrocephalae control medicinal material solution 10 μ l, need testing solution 15 μ l, put respectively on same silica gel g thin-layer plate, with petroleum ether (60-90 ℃)-ethyl acetate (50: 1) is developing solvent, launch, take out, dry, spray (is got vanillin 1g and sulphuric acid 10ml with the vanillin ethanol solution of sulfuric acid, add ethanol to 100ml), in about 10 minutes of 105 ℃ of bakings, in the test sample chromatograph, with control medicinal material solution chromatograph relevant position on, show the speckle of same color.
With the index of said method as the stability of this drug quality control and investigation technology.
Embodiment 2: get Chinese crude drug Massa Medicata Fermentata (stir-fry) 77g, Fructus Aurantii (parched) 39g, the Rhizoma Atractylodis Macrocephalae (stir-fry) 39g, Fructus Crataegi 59g, Fructus Oryzae Germinatus (stir-fry) 192g, Fructus Hordei Germinatus (stir-fry) 192g, above-mentioned raw materials Fructus Oryzae Germinatus, Fructus Hordei Germinatus decoct with water 2 times, and each 30 minutes, collecting decoction, filter, be condensed into the thick paste shape, all the other four flavors are ground into fine powder, with above-mentioned thick paste mixing, add refined honey 115g, make granule, be pressed into 1000, every sheet weight 0.3g:
1, measuring content of ursolic acid with high performance liquid chromatography (appendix VID of Chinese Pharmacopoeia version in 2000) measures and standard:
A. chromatographic condition: with octadecylsilane chemically bonded silica is filler; Methanol 0.1% phosphoric acid solution (80: 18) is a mobile phase, and the detection wavelength is 210nm.
B. the preparation of reference substance solution: precision is got and is claimed that the ursolic acid reference substance is an amount of, adds aqueous methanol and makes the solution that every 1ml contains 35 μ g, promptly.
C. the preparation of need testing solution: get the content under this product content uniformity item, mixing, porphyrize is got 2g, and accurate the title, decide, the accurate aqueous methanol 35ml that adds claims to decide weight, and reflux 1 hour is put cold, weight decided in title again, supplies with methanol to subtract weight loss, filters, promptly.
D. algoscopy: precision is measured reference substance liquid and each 18 μ l of need testing solution respectively, injects chromatograph of liquid, measures, promptly.Every (heavy 0.3g) XIAOER XISHI PIAN contains Fructus Crataegi in ursolic acid, must not be less than 0.10mg.
2. differentiate this medicine Chinese crude drug Rhizoma Atractylodis Macrocephalae with thin layer chromatography:
A. the preparation of control medicinal material solution: take by weighing Rhizoma Atractylodis Macrocephalae control medicinal material 0.8g, add petroleum ether (60-90 ℃) 45ml, supersound extraction 40min filters, and filtrate evaporate to dryness, residue add ethyl acetate 0.5ml makes dissolving, makes control medicinal material solution.
B. the preparation of need testing solution: get 20 of this product, every heavy 0.3 gram grinds, and adds petroleum ether (60-90 ℃) 45ml, and supersound extraction 40min filters, and filtrate evaporate to dryness, residue add ethyl acetate 0.5ml makes dissolving, as need testing solution.
C. test according to thin layer chromatography (appendix VIB of Chinese Pharmacopoeia version in 2000), get above-mentioned Rhizoma Atractylodis Macrocephalae control medicinal material solution 10 μ l, need testing solution 15 μ l, put respectively on same silica gel g thin-layer plate, with petroleum ether (60-90 ℃)-ethyl acetate (50: 1) is developing solvent, launch, take out, dry, spray (is got vanillin 1g and sulphuric acid 10ml with the vanillin ethanol solution of sulfuric acid, add ethanol to 100ml), in about 12 minutes of 105 ℃ of bakings, in the test sample chromatograph, with control medicinal material solution chromatograph relevant position on, show the speckle of same color.
With the index of said method as the stability of this drug quality control and investigation technology.
Embodiment 3: get Chinese crude drug Massa Medicata Fermentata (stir-fry) 77g, Fructus Aurantii (parched) 39g, the Rhizoma Atractylodis Macrocephalae (stir-fry) 39g, Fructus Crataegi 59g, Fructus Oryzae Germinatus (stir-fry) 192g, Fructus Hordei Germinatus (stir-fry) 192g, above-mentioned raw materials Fructus Oryzae Germinatus, Fructus Hordei Germinatus decoct with water 2 times, and each 30 minutes, collecting decoction, filter, be condensed into the thick paste shape, all the other four flavors are ground into fine powder, with above-mentioned thick paste mixing, add refined honey 115g, make granule, be pressed into 1000, every sheet weight 0.3g:
1, measuring content of ursolic acid with high performance liquid chromatography (appendix VID of Chinese Pharmacopoeia version in 2000) measures and standard:
A. chromatographic condition: with octadecylsilane chemically bonded silica is filler; Methanol 0.1% phosphoric acid solution (85: 15) is a mobile phase, and the detection wavelength is 210nm.
B. the preparation of reference substance solution: precision is got and is claimed that the ursolic acid reference substance is an amount of, adds ethanol and makes the solution that every 1ml contains 25 μ g, promptly.
C. the preparation of need testing solution: get the content under this product content uniformity item, mixing, porphyrize is got 1.5g, accurate claims surely, and the accurate ethanol 25ml that adds claims decide weight, and reflux 1 hour is put coldly, claims to decide weight again, supply with methanol to subtract weight loss, filtration, promptly.
D. algoscopy: precision is measured reference substance liquid and each 15 μ l of need testing solution respectively, injects chromatograph of liquid, measures, promptly.Every (heavy 0.3g) XIAOER XISHI PIAN contains Fructus Crataegi in ursolic acid, must not be less than 0.10mg.
2. differentiate this medicine Chinese crude drug Rhizoma Atractylodis Macrocephalae with thin layer chromatography:
A. the preparation of control medicinal material solution: take by weighing Rhizoma Atractylodis Macrocephalae control medicinal material 1g, add petroleum ether (60-90 ℃) 80ml, supersound extraction 50min filters, and filtrate evaporate to dryness, residue add ethyl acetate 0.5ml makes dissolving, makes control medicinal material solution.
B. the preparation of need testing solution: get 25 of this product, every heavy 0.3 gram grinds, and adds petroleum ether (60-90 ℃) 80ml, and supersound extraction 50min filters, and filtrate evaporate to dryness, residue add ethyl acetate 0.5ml makes dissolving, as need testing solution.
C. test according to thin layer chromatography (appendix VIB of Chinese Pharmacopoeia version in 2000), get above-mentioned Rhizoma Atractylodis Macrocephalae control medicinal material solution 20 μ l, need testing solution 20 μ l, put respectively on same silica gel g thin-layer plate, with petroleum ether (60-90 ℃)-ethyl acetate (50: 1) is developing solvent, launch, take out, dry, spray (is got vanillin 1g and sulphuric acid 10ml with the vanillin ethanol solution of sulfuric acid, add ethanol to 100ml), in about 15 minutes of 105 ℃ of bakings, in the test sample chromatograph, with control medicinal material solution chromatograph relevant position on, show the speckle of same color.
With the index of said method as the stability of this drug quality control and investigation technology.
Embodiment 4: get Chinese crude drug Massa Medicata Fermentata (stir-fry) 77g, Fructus Aurantii (parched) 39g, the Rhizoma Atractylodis Macrocephalae (stir-fry) 39g, Fructus Crataegi 59g, Fructus Oryzae Germinatus (stir-fry) 192g, Fructus Hordei Germinatus (stir-fry) 192g, above-mentioned raw materials Fructus Oryzae Germinatus, Fructus Hordei Germinatus decoct with water 2 times, and each 30 minutes, collecting decoction, filter, be condensed into the thick paste shape, all the other four flavors are ground into fine powder, with above-mentioned thick paste mixing, add refined honey 115g, make granule, be pressed into 1000, every sheet weight 0.3g:
1, measuring content of ursolic acid with high performance liquid chromatography (appendix VID of Chinese Pharmacopoeia version in 2000) measures and standard:
A. chromatographic condition: with octadecylsilane chemically bonded silica is filler; Methanol 0.1% phosphoric acid solution (85: 15) is a mobile phase, and the detection wavelength is 210nm.
B. the preparation of reference substance solution: precision is got and is claimed that the ursolic acid reference substance is an amount of, adds to contain water-acetonitrile and make the solution that every 1ml contains 25 μ g, promptly.
C. the preparation of need testing solution: get the content under this product content uniformity item, mixing, porphyrize is got 5g, and accurate the title, decide, contain water-acetonitrile 25ml accurate the adding, claims to decide weight, and reflux 1 hour is put cold, weight decided in title again, supplies with methanol to subtract weight loss, filters, promptly.
D. algoscopy: precision is measured reference substance liquid and each 20 μ l of need testing solution respectively, injects chromatograph of liquid, measures, promptly.Every (heavy 0.3g) XIAOER XISHI PIAN contains Fructus Crataegi in ursolic acid, must not be less than 0.10mg.
2. differentiate this medicine Chinese crude drug Rhizoma Atractylodis Macrocephalae with thin layer chromatography:
A. the preparation of control medicinal material solution: take by weighing Rhizoma Atractylodis Macrocephalae control medicinal material 1g, add petroleum ether (60-90 ℃) 80ml, supersound extraction 50min filters, and filtrate evaporate to dryness, residue add ethyl acetate 0.5ml makes dissolving, makes control medicinal material solution.
B. the preparation of need testing solution: get 25 of this product, every heavy 0.3 gram grinds, and adds petroleum ether (60-90 ℃) 80ml, and supersound extraction 50min filters, and filtrate evaporate to dryness, residue add ethyl acetate 0.5ml makes dissolving, as need testing solution.
C. test according to thin layer chromatography (appendix VIB of Chinese Pharmacopoeia version in 2000), get above-mentioned Rhizoma Atractylodis Macrocephalae control medicinal material solution 20 μ l, need testing solution 20 μ l, put respectively on same silica gel g thin-layer plate, with petroleum ether (60-90 ℃)-ethyl acetate (50: 1) is developing solvent, launch, take out, dry, spray (is got vanillin 1g and sulphuric acid 10ml with the vanillin ethanol solution of sulfuric acid, add ethanol to 100ml), in about 15 minutes of 105 ℃ of bakings, in the test sample chromatograph, with control medicinal material solution chromatograph relevant position on, show the speckle of same color.
With the index of said method as the stability of this drug quality control and investigation technology.
Claims (4)
1. method of quality control for the treatment of the Chinese medicinal tablet of infantile dyspepsia, this Chinese patent medicine is made up of Chinese crude drug stir-fry Massa Medicata Fermentata 77g, Fructus Aurantii (parched) 39g, Rhizoma Atractylodis Macrocephalae (parched) 39g, Fructus Crataegi 59g, stir-fry Fructus Oryzae Germinatus 192g, Fructus Hordei Germinatus (parched) 192g, above-mentioned raw materials is made 1000 altogether, it is characterized in that this method comprises the following steps:
(1), with content of ursolic acid in this medicine of high effective liquid chromatography for measuring:
A. chromatographic condition: with octadecylsilane chemically bonded silica is filler; Proportioning is the methanol of 70-90: 30-10--0.1% phosphoric acid solution is a mobile phase, and the detection wavelength is 210 ± 2nm;
B. the preparation of reference substance solution: it is an amount of that precision takes by weighing the ursolic acid reference substance, adds in methanol or ethanol or the acetonitrile any and make the solution that every 1ml contains 6--60 μ g;
C. the preparation of need testing solution: get the content under this product content uniformity item, mixing, porphyrize, get 0.5--6g, the accurate title, decide, and accurate any 25--100ml that adds in methanol or ethanol or the acetonitrile claims to decide weight, reflux 1 hour, put coldly, claim to decide weight again, supply with methanol or ethanol or acetonitrile and subtract weight loss, filter, promptly;
D. content of ursolic acid assay method: precision is measured reference substance solution and each 5--20 μ l of need testing solution respectively, injects chromatograph of liquid, measures content of ursolic acid;
E. every contains Fructus Crataegi with ursolic acid C
30H
48O
3Meter must not be lower than 0.10mg.
(2), this medicine Chinese crude drug Rhizoma Atractylodis Macrocephalae is differentiated with thin layer chromatography
A. the preparation of control medicinal material solution: take by weighing Rhizoma Atractylodis Macrocephalae control medicinal material 0.25-1g, add 60-90 ℃ petroleum ether 20-100ml, supersound extraction 15-60min filters, and filtrate evaporate to dryness, residue add ethyl acetate 0.5ml makes dissolving, makes control medicinal material solution;
B. the preparation of need testing solution: get this product 8-28 sheet, grind, add 60-90 ℃ petroleum ether 20-100ml, supersound extraction 15-60min filters, and filtrate evaporate to dryness, residue add ethyl acetate 0.5ml makes dissolving, as need testing solution;
C. test according to thin layer chromatography, get above-mentioned Rhizoma Atractylodis Macrocephalae control medicinal material solution 5--20 μ l, need testing solution 5--20 μ l, put respectively on same silica gel g thin-layer plate, with petroleum ether-ethyl acetate of 60-90 ℃ is that developing solvent launches, the two ratio is 50: 1, take out, dry, spray is got vanillin 1g and sulphuric acid 10ml with the vanillin ethanol solution of sulfuric acid, adds ethanol to 100ml, in 105 ℃ of bakings about 5-15 minute, in the test sample chromatograph, with control medicinal material solution chromatograph relevant position on, show the speckle of same color.
2. a kind of method of quality control for the treatment of the Chinese medicinal tablet of infantile dyspepsia according to claim 1 is characterized in that this method comprises the following steps:
(1), with content of ursolic acid in this medicine of high effective liquid chromatography for measuring:
A. chromatographic condition: with octadecylsilane chemically bonded silica is filler; Proportioning is that 87: 13 methanol-0.1% phosphoric acid solution is a mobile phase, and the detection wavelength is 210nm;
B. the preparation of reference substance solution: it is an amount of that precision takes by weighing the ursolic acid reference substance, adds methanol and make the solution that every 1ml contains 25 μ g;
C. the preparation of need testing solution: get the content under this product content uniformity item, mixing, porphyrize is got 1.5g, accurate claims surely, and the accurate methanol 25ml that adds claims decide weight, and reflux 1 hour is put coldly, claims to decide weight again, supply with methanol to subtract weight loss, filtration, promptly;
D. content of ursolic acid assay method: precision is measured reference substance solution and each 20 μ l of need testing solution respectively, injects chromatograph of liquid, measures content of ursolic acid, with control this product quality;
E. every contains Fructus Crataegi with ursolic acid C
30H
48O
3Meter must not be lower than 0.10mg.
(2), this medicine Chinese crude drug Rhizoma Atractylodis Macrocephalae is differentiated with thin layer chromatography
A. the preparation of control medicinal material solution: take by weighing Rhizoma Atractylodis Macrocephalae control medicinal material 0.5g, add 60-90 ℃ petroleum ether 40ml, supersound extraction 30min filters, and filtrate evaporate to dryness, residue add ethyl acetate 0.5ml makes dissolving, makes control medicinal material solution;
B. the preparation of need testing solution: get 18 of this product, grind, add 60-90 ℃ petroleum ether 40ml, supersound extraction 30min filters, and filtrate evaporate to dryness, residue add ethyl acetate 0.5ml makes dissolving, as need testing solution;
C. according to the thin layer chromatography test, get above-mentioned Rhizoma Atractylodis Macrocephalae control medicinal material solution 10 μ l, need testing solution 15 μ l, put respectively on same silica gel g thin-layer plate, with petroleum ether-ethyl acetate of 60-90 ℃ is developing solvent, and the two ratio is 50: 1, launches, take out, dry, spray is with the vanillin ethanol solution of sulfuric acid, get vanillin 1g and sulphuric acid 10ml, add ethanol to 100ml, in about 10 minutes of 105 ℃ of bakings, in the test sample chromatograph, with control medicinal material solution chromatograph relevant position on, show the speckle of same color.
3. a kind of method of quality control for the treatment of the Chinese medicinal tablet of infantile dyspepsia according to claim 1 is characterized in that: selected wavelength 210 ± 2nm is for measuring wavelength.
4. a kind of method of quality control for the treatment of the Chinese medicinal tablet of infantile dyspepsia according to claim 1 is characterized in that: this medicine is a tablet of making every weight 0.3g by the preparation method among second WS3-B-0197-90 of " Drug Standard of Ministry of Public Health of the Peoples Republic of China " Chinese traditional patent formulation preparation.
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| CN109725100B (en) * | 2019-01-08 | 2020-12-25 | 南京海昌中药集团有限公司 | Method for detecting fingerprint spectrum of hawthorn granules |
| CN110596105A (en) * | 2019-09-18 | 2019-12-20 | 贵州医科大学 | Quality standard detection method of wheat malt medicinal material |
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| 中华人民共和国卫生部药品标准中药成方制剂第二册. 中华人和共和国卫生部药典委员会，27. 1991 |
| 中华人民共和国卫生部药品标准中药成方制剂第二册. 中华人和共和国卫生部药典委员会，27. 1991 * |
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