CN100445747C - Method for testing biomolecule based on colloid crystal - Google Patents
Method for testing biomolecule based on colloid crystal Download PDFInfo
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- CN100445747C CN100445747C CNB2005100407377A CN200510040737A CN100445747C CN 100445747 C CN100445747 C CN 100445747C CN B2005100407377 A CNB2005100407377 A CN B2005100407377A CN 200510040737 A CN200510040737 A CN 200510040737A CN 100445747 C CN100445747 C CN 100445747C
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Abstract
The present invention relates to a method for detecting biomacromolecules based on colloidal crystals, which is a simple, convenient and high-flux method for detecting biological samples. A colloidal crystal film is used as a flowing carrier in the process of detecting biomolecules; the method comprises the following steps: step 1, a photonic crystal and pattern combined encoding film is prepared; step 2, nucleic acid or protein probe molecules are fixed; step 3, target molecules are hybridized; step four, the target molecules are identified. An optical fiber spectrometer is used for determining a reflection spectrum, a microscope is used for observing the pattern of the photonic crystal and pattern combined encoding film, and then the combination of the reflectance spectrum band of the colloidal crystal and the photoetched pattern is used as a code; therefore, the biomolecular kinds are identified, and the existence of the target molecules is determined according to fluorescence signals on a target molecule (5). The present invention can be widely used for the technical fields of clinical diagnosis and detection, drug screening, microbial sample detection in environments, animal mating, plant seed breeding, etc.
Description
Technical field
The present invention is used for method to a kind of high throughput testing simply and easily of detecting in the biological sample.Can be widely used in clinical diagnosis and detection, drug screening, fields such as the detection of microbiological specimens in the environment, legal medical expert identify, customs's agricultural product are imported and exported inspection and quarantine, gene order and functional analysis, microorganism is fractal, animals and plants breeding.
Background technology
Along with the arrival of genome epoch and genome times afterwards comprehensively, the detection technique of high-throughout genetic test and protein becomes the basic means and the instrument of this life science fundamental research day by day.The parallel detection of high flux biomolecule can be found new functional gene and protein group expeditiously.Be widely used in the clone of new functional gene, in the research of expression and protein group, and in exploitation new medicine, clinical detection and the animal and plant quarantine, all had wide practical use.At present in the detection of high flux biomacromolecule, it is main that what rely on is be the position encoded array type biochip of carrier dependence and be the flowing carrier liquid-phase chip technology with the fluorescent microsphere with glass sheet etc., but all there is certain defective in these two kinds of technology, though the biochip technology encoding amount is big, but need expensive point sample system, and hybridization speed is slow; And be the liquid-phase chip technology of carrier, thereby owing to adopted dynamic hybridization technique greatly to improve hybridization speed with the fluorescent microsphere.But because technical problem, encoding amount waits to improve.We have proposed with the colloidal crystal colloidal crystal as flowing carrier as macromolecular identify label in the biological detection in this patent, thereby are expected to make the problems referred to above to be resolved.
Colloidal crystal is a kind of of photonic crystal. thereby the periodic arrangement of spheroidal particle can form the propagation therein of optical band gap structure control light in the colloidal crystal. in colloidal crystal, having with the light of optical band gap same frequency and can't be propagated. these incident lights will be reflected by colloidal crystal. on reflectance spectrum, the optical band gap of colloidal crystal shows as a very strong reflection peak. and the position of reflection peak changes with optical band gap. with respect to the colloidal crystal with ordered 3 D structure of other kind, it is easy that colloidal crystal has preparation, advantage such as film forming in the large tracts of land scope easily. we will utilize this optical characteristics of colloidal crystal to realize coding to flowing carrier. and ultimate principle is as follows: at first modify different types of probe molecule on different colloidal crystal carriers, because the position of optical band gap changes with the size of the spheroidal particle of forming colloidal crystal, therefore the kind of fixing biomolecule can be determined by measuring the optical band gap position on any colloidal crystal carrier.When detecting, the colloidal crystal carrier of probe molecule modified is mixed with the detection liquid that contains the target molecule that fluorescence labeling crosses, after probe molecule and target molecule reaction, the kind of probe molecule is determined by the reflectance spectrum of colloidal crystal carrier, and the existence of target molecule and otherwise definite by fluorescence spectrum, colorimetric, chemiluminescence etc.
Bar codes technique is widely used in people's the production and life, bar code is encoded as biomolecule, make the coding of biomolecule make simple, detection mode is directly perceived and convenient, reduced the detection cost, caused many related science workers' attention. in addition, utilize the shape of carrier also can come biomolecule is encoded.
The present invention combines colloidal crystal film coding and bar codes technique or carrier pattern, enlarges the quantity of colloidal crystal coding.
Summary of the invention
Technical matters: the purpose of this invention is to provide a kind of biomacromolecule detection method based on colloidal crystal, when detecting, can determine the kind of probe according to the feature reflectance spectrum of colloidal crystal and bar code or pattern, whether according to the existence that has or not to determine target molecule of hybridization signal.It is fast that speed is hybridized in the present invention, with low cost, is a kind of biological detecting method of high flux simply fast.
Technical scheme: the biomacromolecule detection method based on colloidal crystal of the present invention uses the colloidal crystal film as code carrier in the testing process of biomolecule, and it detects and divides following several steps:
The first step, the making of colloidal crystal and pattern combined coding film: be assembled with the colloidal crystal film after the photoetching on the bottom dimethyl silicone polymer film, form certain pattern on the colloidal crystal film after the photoetching, then at the last top layer dimethyl silicone polymer film that is coated with;
Second step: nucleic acid or protein probe molecule fixing: probe is under the coupled action of silane reagent, with the lower surface of bottom dimethyl silicone polymer film or with top layer dimethyl silicone polymer film upper surface generation covalent coupling, thereby realize of the coupling of colloid epitaxial as flowing carrier and probe molecule;
The 3rd step: the hybridization of target molecule: after nucleic acid or protein sample are handled through purifying or amplification etc., indicate marks such as fluorescence, enzyme, then with the probe molecule hybridization that is fixed on the colloidal crystal film after, the specificity combination takes place, target molecule is combined on the probe molecule;
The 4th step, the identification of target molecule: utilize fiber spectrometer to determine reflectance spectrum, utilize microscope to observe the pattern of film, then with the combination of the reflection bands of a spectrum of colloidal crystal and the pattern after the photoetching as coding, thereby the kind of identification biomolecule, the existence of determining target molecule according to the fluorescence signal on the target molecule whether.The method of colloidal crystal and pattern combined coding is colloidal crystal coding and symbol or shape hybrid coding.The preparation method of colloidal crystal film is: assembling colloidal crystal film carries out photoetching then on the bottom dimethyl silicone polymer film, be coated with top layer dimethyl silicone polymer film on the colloidal crystal film after the photoetching, bottom dimethyl silicone polymer film or top layer dimethyl silicone polymer film are replaced by polystyrene, polymethylmethacrylate, again to bottom dimethyl silicone polymer film or the top layer dimethyl silicone polymer film do not replaced to carry out the surface hydrophilic processing.
When assembling the colloidal crystal film on the bottom dimethyl silicone polymer film, the colloidal crystal film is the polystyrene colloid crystal of different-diameter, or the polymethylmethacrylate colloidal crystal, or the silicon dioxide colloid crystal.
The fixing means of nucleic acid or protein probe molecule is: use earlier radiation grafting, adopt UV ozone or Cement Composite Treated by Plasma technology to handle, utilize silylating reagent to carry out conjugated biological molecules then.
It is that UV ozone produces hydrophilic hydroxyl that bottom dimethyl silicone polymer film surface hydrophilic is handled; Or uv photo initiated grafting, ultraviolet rays causes Raolical polymerizable; Or oxygen plasma treatment, i.e. oxygen ionization in plasma generator produces oxygen anion, ozone, and oxygen radicals etc. can make PDMS surface methyl be converted into hydroxyl, can react initiating agent initiation grafting reaction after the silanization with silylating reagent.
The present invention writes bar code, numeral, letter or other symbols that can distinguish mutually on the colloidal crystal film, or the colloidal crystal film is cut into difformity.Utilize the reflectance spectrum of colloidal crystal and various symbol or shape that flowing carrier is carried out hybrid coding.The big molecular probe of fixed biologically on carrier then is with target material and big molecular hyridization.After the wash-out, utilize the feature reflectance spectrum of mirror based fiber optica spectrophotometer colloidal crystal film, thereby utilize microscope to discern pattern coding identification bioprobe molecule.Detect the signal hybridize on the bioprobe (for example fluorescence signal, chemiluminescent signal, electrochemical signals etc.) then, whether according to the existence that has or not to determine target molecule of hybridization signal.
Fixing and the hybridization of the preparation of code carrier and probe:
(1) preparation: by 10: 1: 10 mass ratioes the preceding aggressiveness of PDMS (dimethyl silicone polymer), hardening agent, normal hexane are mixed, be poured on substrate of glass or the silicon base hot setting.Bottom PDMS surface after the curing carry out hydrophilicity-imparting treatment (UV ozone is handled, with the PDMS surface-CH3 changes into-the OH base, utilize then the silane reagent of band unsaturated link and surface have-hydrolysis reaction takes place, carries out graft reaction again in the PDMS of OH base; Or directly initiation grafting reaction under ultraviolet light; Oxygen plasma treatment generation-OH yl).Utilize solvent evaporation method or vertical czochralski method self assembly on the PDMS film colloidal crystal [PS (polystyrene) colloidal crystal, PMMA colloidal crystal, silicon dioxide colloid crystal etc.] of a certain particle diameter, form the colloidal crystal film.Utilize solvent evaporation method or vertical czochralski method self assembly on the PDMS film, form the colloidal crystal film.The colloidal crystal film is carried out laser ablation, carve different bar codes, other symbols or be carved into different shapes.Then in 5: 1: 5 ratio with PDMS before aggressiveness, hardening agent, normal hexane mix, be poured on the colloidal crystal film, standing over night then with its curing, forms top layer PDMS film.
(2) probe is fixing: the fixing means of double-deck colloidal crystal film is as follows: scheme 1: UV ozone or oxygen plasma treatment PDMS film, APTES (triethoxy aminopropyl silane) and PDMS film reaction, by glutaraldehyde with amido modified nucleic acid probe or antigen, antibodies on the PDMS film.Scheme 2: uv photo initiated grafting acrylic acid or third rare cyanogen (becoming carboxyl after the cyan-hydrolysis), utilize after the NHS activated carboxyl, and then will be connected with the colloidal crystal film with amino probe molecule as coupling agent with EDC (carbon two imido).
(3) hybridization of target molecule: through purify, amplification back and target molecule that identifiable marker arranged be fixed on different films on the different probe molecular mixing after, the concussion acceleration is hybridized.
(4) detect. utilize reflecting spectrograph to detect the reflectance spectrum of colloidal crystal film, utilize microscope to observe figure, both are in conjunction with the kind of determining to determine probe molecule.Utilize fluorescent microscope or spectrophotometric to keep the score not detect the target molecule of fluorescence labeling or chemiluminescent labeling, then whether according to the existence that has or not to determine target molecule of signal.
Beneficial effect: the present invention utilizes colloidal crystal that biomolecule is encoded, thus the dynamic hybridization reaction can realize that target molecule detects the time, and this method is simple.
1, the alternative fluorescent microsphere of the capable sign indicating number of bar or other symbols and shape and colloidal crystal hybrid coding film is encoded and has been reduced the cost of manufacture of coding material, and has simplified detecting instrument, has reduced the detection cost.
2. the hybrid coding film of colloidal crystal and figure coding is compared to fluorescent microsphere coding stable in properties.
3. hybrid coding film, up to ten thousand kinds of biomolecule of codified in theory, hundreds of biomolecule and fluorescent microsphere can only be encoded at present.
4. a kind of as flowing carrier of hybrid coding film compares with planar chip, can accelerate hybridization reaction speed, thereby improve the efficient that detects.
Description of drawings
Fig. 1 be pattern coding with the preparation flow figure of colloidal crystal hybrid coding film and and probe stationary and hybridization after structural drawing.
Fig. 2 is the synoptic diagram of several forms of pattern coding and colloidal crystal bar code combined coding.
Above figure has bottom PDMS 1, colloidal crystal film 2, top layer PDMS film 3, probe 4, target molecule 5.
Embodiment
Fig. 1 be pattern coding with the preparation flow figure of colloidal crystal hybrid coding film and and probe stationary and hybridization after structural drawing.The first step is the perfusion of bottom PDMS 1 on glass sheet or silicon chip, after the curing, strip down from glass sheet, second step was the hydrophilicity-imparting treatment of bottom PDMS1, the 3rd step was a self-organized colloidal crystal film on the bottom PDMS1 of hydrophiling, the 4th step was that photoetching forms the colloidal crystal film 2 with specific morphology, the 5th step was the formation of top layer PDMS film 3, the 6th step was to be target molecules 5 and the hybridization of probe molecule in 4, the seven steps of upper surface stationary probe of the lower surface of bottom PDMS film 1 and top layer PDMS film 3.
Fig. 2 is several forms of pattern coding and colloidal crystal bar code combined coding.The pattern coding can be a bar code, arabic numeral, English alphabet, various figures and difform film.The pattern coding can be a bar code with colloidal crystal bar code combined coding, arabic numeral, English alphabet, the combination of any and colloidal crystal in various figures and difform film coding also can be the combination in any between two kinds of forms or the various ways and the coding of colloidal crystal in the pattern coding.
The pattern coding is seen Fig. 1 with the fixing of film preparation of colloidal crystal hybrid coding and probe with hybridization.
(I) preparation: in 10: 1: 10 ratios the preceding aggressiveness of PDMS (dimethyl silicone polymer), hardening agent, normal hexane are mixed, be poured on substrate of glass or the silicon base hot setting.Hydrophilicity-imparting treatment is carried out on bottom PDMS film 1 surface after the curing, and (UV ozone is handled, and utilizes the silane reagent of band unsaturated link to handle then, carries out graft reaction again; Or directly initiation grafting reaction under ultraviolet light; Oxygen plasma treatment).Colloidal crystal (PS (polystyrene) colloidal crystal with a certain particle diameter, PMMA (polymethylmethacrylate) colloidal crystal, silicon dioxide colloid crystal etc.) utilize solvent evaporation method or vertical czochralski method self assembly on the PDMS film, form the colloidal crystal film, the colloidal crystal film is carried out laser ablation, carve different bar codes, symbol or shape form encoding of graphs 2.Then in 5: 1: 5 ratio with PDMS before aggressiveness, hardening agent, normal hexane mix, be poured on the colloidal crystal film, standing over night then with its curing, forms top layer PDMS film 3.
(II) probe is fixing: the fixing means of double-deck colloidal crystal film is as follows: UV ozone or Cement Composite Treated by Plasma PDMS film, APTES and PDMS film reaction are combined in probe 4 (amido modified nucleic acid or antigen, antibody) on the upper surface of the lower surface of bottom PDMS film 1 and top layer PDMS film 3 by glutaraldehyde.
(III) hybridization of target molecule: through purify, the amplification back and the target molecule 5 of identifiable marker is arranged and different probe molecule 4 on being fixed on different films mixes after, the concussion acceleration is hybridized.
(IV) detect. utilize reflecting spectrograph to detect the reflectance spectrum of colloidal crystal film, utilize microscope to observe figure, both are in conjunction with the kind of determining to determine probe molecule.Utilize fluorescent microscope or spectrophotometric to keep the score not detect the target molecule of fluorescence labeling or chemiluminescent labeling, then whether according to the existence that has or not to determine target molecule of signal.
Claims (6)
1. the biomacromolecule detection method based on colloidal crystal is characterized in that in the testing process of biomolecule, uses the colloidal crystal film as code carrier, and it detects and divides following several steps:
The first step, the making of colloidal crystal and pattern combined coding film: be assembled with the colloidal crystal film (2) after the photoetching on the bottom dimethyl silicone polymer film (1), colloidal crystal film (2) after the photoetching is gone up and is formed certain pattern, then at the last top layer dimethyl silicone polymer film (3) that is coated with;
Second step: nucleic acid or protein probe molecule fixing: probe (4) is under the coupled action of silane reagent, with the lower surface of bottom dimethyl silicone polymer film (1) or with top layer dimethyl silicone polymer film (3) upper surface generation covalent coupling, thereby realize of the coupling of colloid epitaxial as flowing carrier and probe molecule;
The 3rd step: the hybridization of target molecule: after nucleic acid or protein sample are handled through purifying or amplification etc., indicate marks such as fluorescence, enzyme, then with the probe molecule hybridization that is fixed on the colloidal crystal film after, the specificity combination takes place, target molecule (5) is combined on the probe molecule (4);
The 4th step, the identification of target molecule: utilize fiber spectrometer to determine reflectance spectrum, utilize microscope to observe the pattern of film, then with the combination of the reflection bands of a spectrum of colloidal crystal and the pattern after the photoetching as coding, thereby the kind of identification biomolecule, the existence of determining target molecule according to the fluorescence signal on the target molecule (5) whether.
2, the biomacromolecule detection method based on colloidal crystal as claimed in claim 1, the method that it is characterized in that colloidal crystal and pattern combined coding are colloidal crystal coding and symbol or shape hybrid coding.
3, biomacromolecule detection method based on colloidal crystal as claimed in claim 1, it is characterized in that, the preparation method of colloidal crystal film is: bottom dimethyl silicone polymer film (1) is gone up assembling colloidal crystal film (2) and is carried out photoetching then, be coated with top layer dimethyl silicone polymer film (3) on the colloidal crystal film (2) after the photoetching, bottom dimethyl silicone polymer film (1) or top layer dimethyl silicone polymer film (3) are by polystyrene, polymethylmethacrylate is replaced, again to bottom dimethyl silicone polymer film (1) or the top layer dimethyl silicone polymer film (3) do not replaced to carry out the surface hydrophilic processing.
4, the biomacromolecule detection method based on colloidal crystal as claimed in claim 1, it is characterized in that, when bottom dimethyl silicone polymer film (1) is gone up assembling colloidal crystal film (2), colloidal crystal film (2) is the polystyrene colloid crystal of different-diameter, or polymethylmethacrylate colloidal crystal, or silicon dioxide colloid crystal.
5, the biomacromolecule detection method based on colloidal crystal as claimed in claim 1, the fixing means that it is characterized in that nucleic acid or protein probe molecule is: use earlier radiation grafting, adopt UV ozone or Cement Composite Treated by Plasma technology to handle, utilize silylating reagent to carry out conjugated biological molecules then.
6, the biomacromolecule detection method based on colloidal crystal as claimed in claim 3 is characterized in that it is that UV ozone produces hydrophilic hydroxyl that bottom dimethyl silicone polymer film (1) surface hydrophilic is handled; Or uv photo initiated grafting, ultraviolet rays causes Raolical polymerizable; Or oxygen plasma treatment, polymer surfaces produces hydrophilic hydroxyl after the plasma treatment, can react initiating agent initiation grafting reaction after the silanization with silylating reagent.
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| CN101339135B (en) * | 2008-08-18 | 2011-07-27 | 中国科学院化学研究所 | Method for promoting biological detection sensitivity by photon crystal |
| CN101762571B (en) * | 2009-12-30 | 2013-07-10 | 东南大学 | Flow-type gas sensor array and preparation method thereof |
| WO2018035670A1 (en) * | 2016-08-22 | 2018-03-01 | 苏州偲聚生物材料有限公司 | Solid-phase carrier capable of improving detection sensitivity, and detection component |
| CN108444960B (en) * | 2018-02-09 | 2020-04-10 | 清华大学 | Fluorescence correlation spectrum detection method |
| CN111929991A (en) * | 2020-08-10 | 2020-11-13 | 大连理工大学 | Method for rapidly preparing colloidal photonic crystal array chip by utilizing laser direct writing |
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| WO2003098183A2 (en) * | 2002-05-14 | 2003-11-27 | Arryx, Inc. | Broad spectrum optically addressed sensor |
| CN1512179A (en) * | 2002-12-30 | 2004-07-14 | 中国科学院化学研究所 | Particle formwork and particle assembling and transferring method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2003098183A2 (en) * | 2002-05-14 | 2003-11-27 | Arryx, Inc. | Broad spectrum optically addressed sensor |
| CN1512179A (en) * | 2002-12-30 | 2004-07-14 | 中国科学院化学研究所 | Particle formwork and particle assembling and transferring method |
Non-Patent Citations (4)
| Title |
|---|
| 二氧化硅胶体晶体的制备及其光子带隙特性. 快素兰，章俞之，胡行方.无机材料学报，第17卷第1期. 2002 |
| 二氧化硅胶体晶体的制备及其光子带隙特性. 快素兰，章俞之，胡行方.无机材料学报，第17卷第1期. 2002 * |
| 胶体晶体研究进展. 丛海林，曹维孝.化学通报，第2期. 2005 |
| 胶体晶体研究进展. 丛海林，曹维孝.化学通报，第2期. 2005 * |
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