CN100434413C - Method for separating and purifying mandelic acid using wealk alkali anion exchange agent - Google Patents
Method for separating and purifying mandelic acid using wealk alkali anion exchange agent Download PDFInfo
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- CN100434413C CN100434413C CNB2006101352041A CN200610135204A CN100434413C CN 100434413 C CN100434413 C CN 100434413C CN B2006101352041 A CNB2006101352041 A CN B2006101352041A CN 200610135204 A CN200610135204 A CN 200610135204A CN 100434413 C CN100434413 C CN 100434413C
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- amygdalic acid
- acid
- amygdalic
- resin
- anion exchanger
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Abstract
The present invention provides process of separating and purifying mandelic acid with weak alkaline anion exchanger. The process includes the steps of: saturation adsorbing solution of mandelic acid with weak alkaline anion exchanger, water washing to eliminate residual liquid with unexchanged ion, eluting mandelic acid component with eluent solution in certain concentration, decompressing the eluted liquid to recover solvent, vacuum concentrating, and drying to obtain crude mandelic acid product. The process is simple, high in extracting efficiency, and low in production cost.
Description
Technical field
The present invention relates to a kind of separation purification method of chiral drug, more specifically relate to a kind of method that adopts weak base anion exchanger to prepare chiral drugs mandelic acid.
Background technology
Chiral drug is meant the compound medicine of the single enantiomer of pharmacologically active effect.(mandelic acid is a kind of important birth control chiral drug MA) to amygdalic acid, has spermicide and the double effects of the trichomonad of going out, lists Chinese science and technology portion " the Seventh Five-Year Plan ", ministry of Health of China " eight or five " and Fujian Province " 15 " tackling of key scientific and technical problems problem in.Amygdalic acid still is the intermediate feed of many important chiral drugs, with the amygdalic acid be precursor can synthetic vessel expansion cyclome almond ester, urinary tract infections antiphlogistic drug hexamine mandelate, medicine [Chen Jianfeng etc. such as Hydrobenzole and antispasmodic Benzyl Amygdalate put drops in one's eyes, the physicochemical property of chiral drugs mandelic acid is measured, University of Fuzhou's journal, 2005,33 (3): 395~399].
Because amygdalic acid is a chiral molecules, and two kinds of configurations of S-melic acid and R-melic acid are arranged.The amygdalic acid of single configuration is a very important chiral intermediate in the asymmetric catalysis synthesis, is widely used in the asymmetric synthesis of optically pure amino acid, angiotensin converting enzyme inhibitor, coenzyme A.The amygdalic acid of single configuration (or mandelic acid derivatives) institute synthetic medicine is compared with racemic amygdalic acid (or mandelic acid derivatives), and drug effect obviously improves, and toxic side effect significantly descends.
At present, the amygdalic acid of selling on market has S-melic acid, R-melic acid or amygdalic acid raceme etc.The amygdalic acid demand is approximately with average annual 10% speed increment on the world market, and Chinese amygdalic acid consumption was about 250 tons [Zhang Nan, chipal compounds amygdalic acid DEVELOPMENT PROSPECT is wide, Chinese chemical industry newspaper, 2002-2-21] in 2000.Therefore, the preparation method of research amygdalic acid has certain directive significance and vast market prospect.
Ion exchange technique is to utilize the strong and weak difference of ion-exchanger and various ionic reactive force, optionally adsorbs or discharges specific ion, thereby reach the purpose of removing impurity, enrichment or purification of target biochemical substances.Ion-exchanger commonly used has two classes: hydrophobic structure ion-exchanger and hydrophilic-structure ion-exchanger.The former is usually said ion exchange resin, be raw material mainly with materials such as vinylbenzene, vinylformic acid or phenolic aldehyde, through synthetic solid-state polymer compound is the hydrophobicity skeleton, has the physical strength height, the water-swellable rate is low, characteristics such as exchange capacity is big, small-molecule substances such as organic acid, microbiotic should use the ion exchange resin of hydrophobic structure to separate.
Ion exchange resin is a kind of general soda acid and organic solvent of being insoluble to, also fused solid-state polymer compound not, and good stability not only, but and have a multi-functional base of ion-exchange.It is by insoluble three-dimensional space mesh skeleton, be connected functional group on the skeleton and functional group with the modular constructions such as exchangable ion of opposite charges form.From the acid-basicity viewpoint, ion exchange resin can be considered multi-functional macromolecular multi-component acid (H
+) or macromolecular multi-component alkali (OH
-).
Active ion in the commutative functional group of ion exchange resin determines the salient features of this resin, and the degree of ionization of functional group has determined the acid or alkaline power of resin.Press the functional group branch, usually resin is divided into strongly-acid, weakly acidic cation-exchange resin and four big classes such as strong basicity, weak base anion-exchange resin, four big class ion exchange resin performances relatively see Table 1.
Table 1 four big class ion exchange resin performances relatively
The salient features of ion exchange resin has: degree of crosslinking, rate of expansion, water content, ion-exchange speed, exchange capacity and exchange selectivity etc.Usually, influence the Ion-exchange Selectivity factor potential of hydrogen of ion hydration radius, ionic valency and solution etc. is arranged.Avidity between ion and the resin is big more, just easy being adsorbed more; Under low concentration aqueous solution and normal temperature condition, the ionic valency is high more, just easy being adsorbed more; In the exchange of mineral ion, the easy more absorption of ion that hydrated radius is less.Various monovalent ions are as follows to the avidity size ordering of resin: when adopting basic resin, and OH
-<F
-<HCO
3 -<Cl
-<HSO
3 -<Br
-<NO
3 -<I
-<ClO
4 -When adopting weakly base resin, F
-<HCO
3 -<Cl
-<HSO
3 -<Br
-<NO
3 -<I
-<ClO
4 -<OH
-
At present, rosin products is of a great variety in the world wide, and sorting technique also has nothing in common with each other.The main weak base anion-exchange resin commodity of domestic production circulation then mainly are weak base anion-exchange resin products such as polystyrene, acrylic acid series, phenolic aldehyde system, epoxy system, vinylpyridine system, urea aldehyde system and vinyl chloride according to the skeleton structure of resin; Brand according to resin is divided, import brand has the Amberlite IR series of Rhom and Hass (as Amberlite IR-4B, Amberlite IRA-45, Amberlite IRA-93, Amberlite IRA-94), domestic brand has the D315 of East China University of Science, D345 and 335 weak base anion-exchange resin products, 300 series that Chemical Plant of Nankai Univ. and Shanghai Resin Factory produce are [as 331 (trade(brand)names 701), 303 * 2 (trade(brand)names 704), D311 (trade(brand)name 703), D301 (trade(brand)name 710,370) and D302 (trade(brand)name 390)] etc. the weak base anion-exchange resin product.
Because have carboxylic acid in the amygdalic acid molecule, its dissociation constant pKa value is 5.28, is the slightly acidic organic acid.According to the ion-exchange theory, under acidic conditions, amygdalic acid mainly exists with molecularity, can select for use macroporous adsorbent resin to carry out enriching and purifying, but under neutrality or alkaline condition, amygdalic acid then is to exist with the negatively charged ion state, should adopt ion exchange technique to separate amygdalic acid.
Adopting the main foundation of ion exchange technique separation and purification amygdalic acid is that ion exchange resin is different to the avidity of amygdalic acid and impurity.Ion exchange resin depends primarily on the influence of other impurity in the functional group characteristics of physico-chemical property, resin of amygdalic acid and the solution to the avidity of amygdalic acid.Because amygdalic acid is the slightly acidic organic acid, under neutrality or alkaline condition, is to exist with the negatively charged ion state, according to the ion-exchange theory, can select for use anionite-exchange resin to carry out separation and Extraction.
Owing to except that mandelate ion, also have Cl in the amygdalic acid fermented liquid
-, SO
4 2-, PO
4 3-Deng negatively charged ion, and amphotericeledrolyte such as the amino acid that under neutrality or alkaline condition, exists, protein with the negatively charged ion state, when selecting resin to carry out separation and purification, can take 2 strategies: 1. select mineral ion and foreign protein avidity stronger, and the resin more weak to amygdalic acid avidity, impurity is attracted on the resin when upper prop, and amygdalic acid flows out in a large number, play the purpose that amygdalic acid and impurity separate.2. or on the contrary, selection is stronger to amygdalic acid avidity, and to more weak resins of impurity avidity such as mineral ion, amino acid and protein, amygdalic acid is adsorbed on the resin in a large number, mode by desorption then is with certain density eluent wash-out amygdalic acid component.1. plant strategy and compare with the, 2. the plant the high efficiency separation that strategy not only can reach amygdalic acid and impurity, and the amygdalic acid component is had tangible enrichment concentrated effect.
When adopting weak base anion exchanger separation and purification amygdalic acid, various mineral ions are as follows to the avidity size ordering of resin: MA
-<F
-<Cl
-<HSO
3 -<Br
-<NO
3 -<I
-<ClO
4 -<OH
-<SO
4 2-<pO
4 3-, therefore, can adopt and contain F
-, Cl
-, Br
-, I
-, OH
-, SO
4 2-, PO
4 3-Eluent Deng mineral ion carries out wash-out, and this eluent can be acidity, neutrality or basic solution, preferentially selects for use certain density HCl to carry out wash-out.
Summary of the invention
The object of the present invention is to provide a kind of method that adopts weak base anion exchanger separation and purification amygdalic acid, not only equipment is simple for this method, good separating effect, product yield height, and resin long service life, production cost are low.
The method of employing weak base anion exchanger separation and purification amygdalic acid of the present invention is achieved in that the solution that contains amygdalic acid, be adsorbed to saturated with weak base anion exchanger, the debris of ion-exchange does not take place in the water flush away, use certain density eluent wash-out amygdalic acid component again, elutriant through decompression and solvent recovery, vacuum concentration, be dried to the amygdalic acid crude product.
The present invention preferentially select for use HCl be the advantage of eluent be mainly following some:
1. compare with other halogen acid such as HF, HBr, HI, HCl is cheap and easy to get, can obviously reduce the production cost of amygdalic acid as eluent.
2. because amygdalic acid can only be dissociated into univalent anion at most, amygdalic acid and eluent are to wait mole to exchange, as employing H
2SO
4, H
3PO
4When being eluent,, cause H easily though also can reach the purpose of wash-out amygdalic acid component Deng polyvalent mineral acid
+Excessive release and accumulation, be unfavorable for the accurate judgement of wash-out terminal point.
3. when adopting weak base anion exchanger separation and purification amygdalic acid, waiting under the volumetric molar concentration condition, each mineral ion is as follows to the avidity size ordering of resin: MA
-<F
-<Cl
-<HSO
3 -<Br
-<NO
-<I
-<ClO
4 -<OH
-<SO
4 2-<PO
4 3-, adopt to contain Cl
-Acidic solution can reach the purpose of wash-out amygdalic acid component.
4. neutrality or the basic solution that contains other mineral ion with employing is that eluent is compared, and adopting HCl solution is that eluent not only can reach the purpose of wash-out amygdalic acid component, and helps adopting simultaneously elutriant pH value rapid drawdown and acid AgNO
3The foundation of the sedimentary variation characteristic of AgCl as the wash-out endpoint appears in indicator.
Preparation method's of the present invention major advantage is: made full use of weak base anion exchanger to the avidity of targeted activity material amygdalic acid and difference to the avidity of impurity such as protein, polysaccharide, amino acid, pigment, inorganic salt, and certain density eluent solution different to the elutive power that is adsorbed on amygdalic acid and impurity such as protein, amino acid, inorganic salt on the ion exchange resin, really reached the high efficiency separation of amygdalic acid and impurity.
Embodiment
The present invention adopts the method for weak base anion exchanger separation and purification amygdalic acid, its concrete steps are: the solution that contains amygdalic acid, under pH value 5.7~6.8 conditions, be adsorbed to saturated with weak base anion exchanger, go not take place the debris of ion-exchange earlier with the washing that is no less than 1 times of amount of resin (V/V), use the eluent of 0.25~0.75mol/L concentration of 4~9 times of amount of resin (V/V) again, with 1~3 times of amount of resin/hour flow velocity (V/V) wash-out amygdalic acid component, elutriant-0.06~-0.095MPa, under 50~80 ℃ of conditions, through decompression and solvent recovery, vacuum concentration, be dried to the amygdalic acid crude product.
Wherein, the solution of used amygdalic acid can be the mandelic acid extract that derives from plant extract, derive from biosynthetic amygdalic acid fermented liquid, derive from enzyme catalysis or fractionation the amygdalic acid reaction solution, derive from the amygdalic acid reaction solution of chemosynthesis or fractionation one or more.
Amygdalic acid can be one or more in S-melic acid, R-melic acid or the racemize amygdalic acid.
The chemical constitution of weak base anion exchanger skeleton is one or more in polystyrene, polyacrylic acid or the phenolic aldehyde, preferentially selects polystyrene or polyacrylic acid for use.
It is OH that the negatively charged ion of eluent is formed
-, F
-, Cl
-, Br
-, I
-, SO
4 2-, PO
4 3-Deng in the mineral ion one or more, eluent can be one or more in acid, neutrality or the basic solution, preferentially selects HCl for use.
The physical and chemical parameter measuring method of prepared product of the present invention is as follows:
(1) amygdalic acid Determination on content: adopt high performance liquid chromatography.Condition determination: Agilent 1100 type high performance liquid chromatographs (DAD diode-array detector), Waters Nova-Pak C
18Chromatographic column (Φ 4.6 * 250mm, 5 μ m), moving phase is 50mmol/L phosphate buffered saline buffer (pH6.8): methyl alcohol=9: 1 (V/V), flow velocity 1.0ml/min, 30 ℃ of column temperatures, sample size 20 μ L detect wavelength 220nm.(available from Sigma company) is contrast with the amygdalic acid raceme.
(2) mensuration of the optical antipode content of amygdalic acid (e.e value): adopt capillary electrophoresis.Deposition condition: neutral molten silicon capillary column (I.D.75 μ m, useful length 50cm), damping fluid is the 100mmol/L Tris phosphoric acid solution (pH 7.6) that contains the 150g/L hydroxypropyl-beta-cyclodextrin, separation voltage 20kV, separate 20 ℃ of column temperatures, sample introduction pressure 2.76 * 10
3Pa, sample injection time 8sec, ultraviolet detection wavelength 214nm is contrast with R-amygdalic acid and S-amygdalic acid standard substance (available from Sigma company).Standard substance are prepared (Milli.-QII type ultrapure water system) with ultrapure water, and concentration is 0.75mmol/L.Kapillary pre-treatment: use ultrapure water (13.78 * 10 respectively before each the use
4Pa) drip washing 3min, 0.1mol/L NaOH (6.89 * 10
4Pa) drip washing 2min, ultrapure water drip washing 3min.Use ultrapure water and each drip washing 2min of damping fluid before each sample introduction.The calculation formula of enantiomeric excess value: e.e.=([R]-[S])/([R]+[S]) * 100%, wherein: [R] and [S] is the content (mmol/L) of R type and S type amygdalic acid.
(3) determination of polysaccharide adopts the phenolsulfuric acid method, is contrast with glucose or D-semi-lactosi.Protein content determination adopts FoLin-phenol method, is contrast with the bSA.Inorganic ion content is measured and is adopted kit measurement, wherein SO
4 2-Mensuration adopt the bariumchloride precipitator method, Cl
-Measure and adopt the Silver Nitrate precipitator method, Ca
2+And Mg
2+Mensuration adopt methyl thymol blue complexometry.
The drying means of prepared product of the present invention is as follows:
(1) the spraying drying condition is: feeding liquid concentration 10~20 degree Beaume (60 ℃), 160~250 ℃ of PG-5 type spray-drier inlet temperatures, 60~110 ℃ of temperature outs, centrifugal head operating pressure 1.6~3.0kgf/cm
2
(2) lyophilize condition is: drying temperature-10~-60 ℃, 35~70 ℃ of sublimation temperatures, pressure 0.05~0.18mbar, time of drying 20~40h.
(3) vacuum-drying condition is: 45~75 ℃ of drying temperatures, pressure-0.06~-0.095MPa, time of drying 15~50h.
Preparation method's of the present invention embodiment is presented below:
Embodiment 1
The pH value is 5.78, R, S-amygdalic acid concentration is the fermented liquid of 98.6mmol/L, adopt Amberlite IRA-45 anionite-exchange resin to be adsorbed to saturated, earlier with water (V/V) the flush away polar impurity that is no less than 1 times of amount of resin, use the 0.75mol/L HCl solution (V/V) of 4 times of amount of resin again, with 2 times of amount of resin/hour flow velocity (V/V) wash-out amygdalic acid component, elutriant-0.06~-0.095MPa, 50~80 ℃ of conditions under, become the amygdalic acid crude product through decompression and solvent recovery, vacuum concentration, vacuum-drying.After measured, the rate of recovery 86.7% of amygdalic acid, the purity of amygdalic acid are 55.1%.
Embodiment 2
R-amygdalic acid concentration is the peach leaf extracting solution of 123.6mmol/L, behind hydrochloric acid or sodium hydroxide solution adjust pH to 6.82, adopt Amberlite IRA-93 anionite-exchange resin to be adsorbed to saturated, earlier with water (V/V) the flush away polar impurity that is no less than 1 times of amount of resin, use the 0.6mol/L HCl solution (V/V) of 8 times of amount of resin again, with 3 times of amount of resin/hour flow velocity (V/V) wash-out amygdalic acid component, elutriant-0.06~-0.095MPa, 50~80 ℃ of conditions under, become the amygdalic acid crude product through decompression and solvent recovery, vacuum concentration, vacuum-drying.After measured, the rate of recovery 90.6% of amygdalic acid, the purity of amygdalic acid are 54.3%.
Embodiment 3
S-amygdalic acid concentration is the almond eyeball enzyme hydrolyzate of 213.8mmol/L, behind hydrochloric acid or sodium hydroxide solution adjust pH to 6.59, adopt Amberlite IR-4B anionite-exchange resin to be adsorbed to saturated, earlier with water (V/V) the flush away polar impurity that is no less than 1 times of amount of resin, use the 0.25mol/L HCl solution (V/V) of 9 times of amount of resin again, with 1 times of amount of resin/hour flow velocity (V/V) wash-out amygdalic acid component, elutriant-0.06~-0.095MPa, 50~80 ℃ of conditions under, become the amygdalic acid crude product through decompression and solvent recovery, vacuum concentration, vacuum-drying.After measured, the rate of recovery 86.5% of amygdalic acid, the purity of amygdalic acid are 54.8%.
Embodiment 4
The pH value is 6.53, R-amygdalic acid concentration is the fermented liquid of 125.7mmol/L, and it is saturated to adopt D315 anionite-exchange resin to be adsorbed to, and earlier with water (V/V) the flush away polar impurity that is no less than 1 times of amount of resin, uses the 0.4mol/L NH of 8 times of amount of resin again
4Cl solution (V/V), with 3 times of amount of resin/hour flow velocity (V/V) wash-out amygdalic acid component, elutriant-0.06~-0.095MPa, 50~80 ℃ of conditions under, through decompression and solvent recovery, vacuum concentration, be spray dried to the amygdalic acid crude product.After measured, the rate of recovery 85.6% of amygdalic acid, the purity of amygdalic acid are 56.7%.
Embodiment 5
S-amygdalic acid concentration is the peach leaf extracting solution of 163.5mmol/L, behind hydrochloric acid or sodium hydroxide solution adjust pH to 5.74, it is saturated to adopt D345 anionite-exchange resin to be adsorbed to, and earlier with water (V/V) the flush away polar impurity that is no less than 1 times of amount of resin, uses the 0.75mol/L NH of 7 times of amount of resin again
4Cl solution (V/V), with 1 times of amount of resin/hour flow velocity (V/V) wash-out amygdalic acid component, elutriant-0.06~-0.095MPa, 50~80 ℃ of conditions under, through decompression and solvent recovery, vacuum concentration, be spray dried to the amygdalic acid crude product.After measured, the rate of recovery 86.3% of amygdalic acid, the purity of amygdalic acid are 57.0%.
Embodiment 6
R, S-amygdalic acid concentration is the almond eyeball enzyme hydrolyzate of 189.4mmol/L, behind hydrochloric acid or sodium hydroxide solution adjust pH to 6.83, it is saturated to adopt 335 anionite-exchange resin to be adsorbed to, with water (V/V) the flush away polar impurity that is no less than 1 times of amount of resin, use the 0.25mol/L NH of 9 times of amount of resin more earlier
4Cl solution (V/V), with 2 times of amount of resin/hour flow velocity (V/V) wash-out amygdalic acid component, elutriant-0.06~-0.095MPa, 50~80 ℃ of conditions under, through decompression and solvent recovery, vacuum concentration, be spray dried to the amygdalic acid crude product.After measured, the rate of recovery 83.4% of amygdalic acid, the purity of amygdalic acid are 52.6%.
Embodiment 7
The pH value is 6.12, S-amygdalic acid concentration is the fermented liquid of 156.3mmol/L, behind sodium hydroxide solution adjust pH to 6.87, it is saturated to adopt 701 anionite-exchange resin to be adsorbed to, earlier with water (V/V) the flush away polar impurity that is no less than 1 times of amount of resin, use the 0.25mol/L NaOH solution (V/V) of 9 times of amount of resin again, with 1 times of amount of resin/hour flow velocity (V/V) wash-out amygdalic acid component, elutriant-0.06~-0.095MPa, 50~80 ℃ of conditions under, become the amygdalic acid crude product through decompression and solvent recovery, vacuum concentration, lyophilize.After measured, the rate of recovery 85.9% of amygdalic acid, the purity of amygdalic acid are 55.7%.
Embodiment 8
R, S-amygdalic acid concentration is the peach leaf extracting solution of 99.8mmol/L, behind hydrochloric acid or sodium hydroxide solution adjust pH to 6.53, it is saturated to adopt 704 anionite-exchange resin to be adsorbed to, earlier with water (V/V) the flush away polar impurity that is no less than 1 times of amount of resin, use the 0.5mol/L NaOH solution (V/V) of 8 times of amount of resin again, with 2 times of amount of resin/hour flow velocity (V/V) wash-out amygdalic acid component, elutriant-0.06~-0.095MPa, 50~80 ℃ of conditions under, become the amygdalic acid crude product through decompression and solvent recovery, vacuum concentration, lyophilize.After measured, the rate of recovery 87.6% of amygdalic acid, the purity of amygdalic acid are 53.3%.
Embodiment 9
R-amygdalic acid concentration is the almond eyeball enzyme hydrolyzate of 175.6mmol/L, behind hydrochloric acid or sodium hydroxide solution adjust pH to 5.75, it is saturated to adopt 710 anionite-exchange resin to be adsorbed to, earlier with water (V/V) the flush away polar impurity that is no less than 1 times of amount of resin, use the 0.75mol/L NaOH solution (V/V) of 4 times of amount of resin again, with 3 times of amount of resin/hour flow velocity (V/V) wash-out amygdalic acid component, elutriant-0.06~-0.095MPa, 50~80 ℃ of conditions under, become the amygdalic acid crude product through decompression and solvent recovery, vacuum concentration, lyophilize.After measured, the rate of recovery 88.6% of amygdalic acid, the purity of amygdalic acid are 52.0%.
Above embodiment is intended to further describe for example the present invention, rather than limits the present invention by any way.
The present invention is novel, and technology is simple, the extraction efficiency height, and production cost is low, has bigger dissemination.
Claims (4)
1. method that adopts weak base anion exchanger separation and purification amygdalic acid, it is characterized in that: the solution that contains amygdalic acid, be adsorbed to saturated with weak base anion exchanger, the debris of ion-exchange does not take place in the water flush away, use certain density eluent wash-out amygdalic acid component again, elutriant through decompression and solvent recovery, vacuum concentration, be dried to the amygdalic acid crude product; Concrete step is: the solution that contains amygdalic acid, under pH value 5.7~6.8 conditions, be adsorbed to saturated with weak base anion exchanger, go not take place the debris of ion-exchange earlier with the washing that is no less than 1 times of amount of resin (V/V), use the eluent of 0.25~0.75mol/L concentration of 4~9 times of amount of resin (V/V) again, with 1~3 times of amount of resin/hour flow velocity (V/V) wash-out amygdalic acid component, elutriant-0.06~-0.095MPa, 50~80 ℃ of conditions under, through decompression and solvent recovery, vacuum concentration, be dried to the amygdalic acid crude product; It is OH that the negatively charged ion of described eluent is formed
-, F
-, Cl
-, Br
-, I
-In the mineral ion one or more.
2. the method for employing weak base anion exchanger separation and purification amygdalic acid according to claim 1 is characterized in that: the chemical constitution of described weak base anion exchanger skeleton is one or more in polystyrene, polyacrylic acid or the phenolic aldehyde.
3. the method for employing weak base anion exchanger separation and purification amygdalic acid according to claim 1 is characterized in that: described amygdalic acid is one or more in S-melic acid, R-melic acid or the racemize amygdalic acid.
4. the method for employing weak base anion exchanger separation and purification amygdalic acid according to claim 1 is characterized in that: the described material that contains amygdalic acid is the mandelic acid extract that derives from plant extract, derive from biosynthetic amygdalic acid fermented liquid, derive from enzyme catalysis or fractionation the amygdalic acid reaction solution, derive from the amygdalic acid reaction solution of chemosynthesis or fractionation one or more.
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