CN100425250C - Isatis root extract and its preparation method - Google Patents
Isatis root extract and its preparation method Download PDFInfo
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- CN100425250C CN100425250C CNB2005100127677A CN200510012767A CN100425250C CN 100425250 C CN100425250 C CN 100425250C CN B2005100127677 A CNB2005100127677 A CN B2005100127677A CN 200510012767 A CN200510012767 A CN 200510012767A CN 100425250 C CN100425250 C CN 100425250C
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Abstract
The present invention relates to a traditional Chinese medicine extract product, more specifically to an extract of isatis roots. The present invention solves the problems of no concentration of various hydrotropic and fat-soluble effective ingredients, incomprehensive extraction position and low transfer rate of the effective ingredients in the extract of the isatis roots. The extract of the isatis roots is made by the following preparation steps: isatis root decoction pieces are taken, ethanol solution with the density of 50 to 85% is added and is placed in a proper container, and the amount of the ethanol solution is 5 to 15 times the amount of the isatis root decoction pieces; the proper container is sealed and soaked in a cold way, the isatis root decoction pieces are heated, reflowed, extracted and filtered, alcohol is recovered in the mode of decompression, and medicinal liquid is obtained; the vacuum degree is controlled to be from 20 to 90kPa, and a thick paste is obtained by the decompression and the concentration of the medicinal liquid; the thick paste is dried at the temperature of 50 to 80DEGC, and a product is obtained. The extract of the isatis roots of the present invention has the obvious action of fever removal, antiinflammation, analgesia, virus resistance, etc. and is obviously superior to the medicinal effects of isatis root granules, isatis root tea, etc. which are collected and recorded in 'China Pharmacopoeia' published in 2005, the distribution of the effective ingredients of the isatis roots is comprehensively considered in the present invention, and the effective ingredients in a hydrotropic position and a fat-soluble position are extracted to the great extent.
Description
Technical field
The present invention relates to a kind of Chinese medicine extract, specifically is a kind of Radix Isatidis extract and preparation method thereof.
Background technology
Radix Isatidis is one of China's traditional heat-clearing and detoxifying herb commonly used for many years, in state-owned cultivation widely, the consecutive numbers version is formally recorded in the country-level drug standard of China (" Chinese pharmacopoeia version in 2005, a p142).
It has antiviral, antiendotoxin, analgesic, analgesia, antiinflammatory, blood circulation promoting and blood stasis dispelling, anticancer effect.The above-mentioned effect of Radix Isatidis is mainly from its contained water-soluble amino acid, polysaccharide and fat-soluble indirubin, effective ingredient such as indigo.The main production of Radix Isatidis preparation is that the Radix Isatidis water is decocted at present, the decoction liquor that merges is concentrated, and then adding ethanol, leave standstill, get supernatant, concentrate, dry, clear paste, in clear paste, add suitable adjuvant, make different Radix Isatidis preparations, as isatis root tea and Radix Isatidis granule (" p487 of Chinese pharmacopoeia version in 2005).The problem that this kind technology exists be obviously cause the fat-soluble effective ingredient indirubin of Radix Isatidis, it is indigo etc. that to put forward rate extremely low.Application number is 03110306 to disclose the patent of invention of a kind of Radix Isatidis extract composition and method of making the same and purposes, the Radix Isatidis extract compositions that obtains with the described method of this patent.Wherein the total alkali extractive content of Radix Isatidis is a 50-90% weight, and the content of Beilstein (a kind of alkaloid) in total composition is 10-30% weight.”
Because of the above patented method is to be purpose with the enrichment total alkaloids, then must cause losing in a large number of aminoacid, polysaccharide, organic acid, sterols and other effective ingredient; Run counter to the composing principle of Chinese medicine four gas, the five tastes, lifting to a certain extent; Beilstein has improperly as the quality detecting index of Radix Isatidis total alkaloids also more, because of quality detecting index should be selected the corresponding to main effective ingredient with this Chinese medicine card marquis in principle, and does not see clearly report about the pharmacological action of Beilstein; " it is complicated that the Radix Isatidis preparation technology recorded of Chinese pharmacopoeia shows, and with China most herbal pharmaceutical enterprise existence conditions certain distance arranged still, if this method of use certainly will bring investment bigger to pharmacy corporation, the higher problem of cost than current edition for this method; Long in the production cycle, under the high condition of cost, the result that obtains, it is the drug effect of medicine, only " be better than general Radix Isatidis decoction pieces ", and do not show whether be better than current edition " Radix Isatidis preparation that Chinese pharmacopoeia is recorded is as the drug effect of indigowoad root granules or isatis root tea.
Summary of the invention
The present invention does not concentrate in order to solve water solublity in the blue root extract that exists in the prior art and fat-soluble multiple active ingredient, and extract part is not comprehensively and the low problem of the active ingredient rate of transform and a kind of Radix Isatidis extract and preparation method thereof is provided.
The present invention is realized by following technical scheme, a kind of Radix Isatidis extract, and it is to be made by following preparation steps:
Radix Isatidis extract of the present invention is only compared for the contained a part of alkaloid of Radix Isatidis with extract that the described extraction process of publication application documents obtains, considered the distribution of effective ingredient of isatis root, the effective ingredient with water solublity and fat-soluble two positions proposes to greatest extent comprehensively.Simultaneously, because operating procedure of the present invention is simple, needn't use complicated operations such as chromatographic column, macroporous resin adsorption post, recrystallization, purification step that loss amount is bigger, and technology is fit to the production equipment present situation of China herbal pharmaceutical enterprise very much, help large-scale promotion application, improve the people ' s health level.
This extract comprises water solublity arginine, proline, glutamic acid, γ-An Jidingsuan, valine, tyrosine, leucine and fat-soluble indirubin and indigo.The blue root extract toxicity that the present invention obtains is low, few side effects, high dose use no obvious adverse reaction.
The beneficial effect of the extracting method of Radix Isatidis extract of the present invention, to checking indirubin, indigo with TLC (thin layer chromatography) method by the Radix Isatidis granule of Chinese Pharmacopoeia decoction alcohol precipitation method preparation and the Radix Isatidis extract matter of utilizing method of the present invention to prepare, (immobile phase is the 0.3-0.5%CMC-Na silica gel g thin-layer plate, when developing solvent is toluene-chloroform-acetone (5: 4: 1), can be to indirubin, indigoly carry out sxemiquantitative and detect.) shown in accompanying drawing 1 (in conjunction with coloured picture), utilize Radix Isatidis extract indirubin (pink speckle), the indigo fat-soluble component contents such as (light blue speckles) of method preparation of the present invention high at least 20 times than prior art.
To the Radix Isatidis granule by Chinese Pharmacopoeia decoction alcohol precipitation method preparation with utilize Radix Isatidis extract matter of the present invention to detect total amino acids content with the TLC method, (immobile phase is the 0.3-0.5%CMC-Na silica gel g thin-layer plate, during n-butyl alcohol-glacial acetic acid-water (19: 5: 5), multiple water-soluble amino acid such as arginine are carried out sxemiquantitative detect.) as shown in Figure 2, point 4 is the Radix Isatidis granule of Chinese Pharmacopoeia decoction alcohol precipitation method preparation, point 7 is for utilizing Radix Isatidis extract matter of the present invention.The extraction ratio of total amino acids water soluble ingredient is much higher than prior art.The experiment concrete outcome is as shown in the table:
The different extraction processes of table 1 are to the influence of the indirubin and the arginine rate of transform
| Extraction process | The indirubin rate of transform (%) | The L-arginine rate of transform (%) |
| Decoction alcohol precipitation method | 3.5 | 62.3 |
| Method of the present invention | 70.4 | 66.7 |
| This patent method of merceration not | 50.6 | 45.8 |
Under the prerequisite of having determined optimum extraction process, investigated of the influence of different extraction process methods again to the indirubin in the Radix Isatidis and the arginine rate of transform.Having investigated existing pharmacopeia indigowoad root granules preparation technology is the extract that decoction alcohol precipitation method obtains, and extraction method of the present invention obtains extract and without the indirubin and the arginic rate of transform in the method extract of the present invention of merceration.
That Radix Isatidis extract of the present invention has is analgesic significantly, antiinflammatory, analgesia, antivirus action etc., obviously is better than " the drug effect of the indigowoad root granules that records of Chinese pharmacopoeia version in 2005, isatis root tea etc.
A. choose the Radix Isatidis decoction pieces, add 50～85% alcoholic solution of 5～15 times of weight of decoction pieces, place suitable vessel, airtight, merceration;
B. heating and refluxing extraction;
C. filter, decompression recycling ethanol gets medicinal liquid;
D. medicinal liquid is evaporated to and obtains thick paste, obtain Radix Isatidis extract of the present invention.
Can also be further with thick paste in 50～80 ℃ of dryings, obtain Radix Isatidis extract of the present invention, so that preserve or transportation.
The practical range of the best of the present invention is: the temperature of described step a merceration is 10～30 ℃, and the time is 1～24 hour, preferred 70% ethanol, 12～24 hours merceration time.The operation of described step b repeats 1～4 time, and each heating and refluxing extraction time was controlled at 0.5～5 hour, and the concentration of alcohol after the merging is 50～70%; Preferred repetition 1～3 time, each 0.5～2 hour.Step c heating and temperature control is at 50～80 ℃.Vacuum degree control is at 20～90kPa; The relative density that the thick paste that described steps d obtains records in the time of 50 ℃ is 1.25-1.35.
Thick paste further obtains product under the condition of drying under reduced pressure, vacuum degree control is in 20～90kPa operation, and drying can be passed through water bath method, and then oven dry.
A kind of preparation method of Radix Isatidis extract, it comprises following preparation steps:
A. choose the Radix Isatidis decoction pieces, add 50～85% alcoholic solution of 5～15 times of weight of decoction pieces, place container, airtight, merceration; The temperature of merceration is 10～30 ℃, and the time is 1～24 hour, preferred 70% ethanol, 12～24 hours merceration time;
B. the container heating and refluxing extraction is obtained the alcoholic solution of medicine; Operation repeats 1～4 time, and each heating and refluxing extraction time was controlled at 0.5～5 hour.Preferred repetition 1～3 time, each 0.5～2 hour;
C. filter,, get the aqueous solution of medicine extracting solution heating recovery ethanol; Heating and temperature control is at 50～80 ℃, and vacuum degree control is at 20～90kPa;
D. medicinal liquid is evaporated to and obtains thick paste; The relative density that the thick paste that obtains records in the time of 50 ℃ is 1.25-1.35;
Promptly obtain Radix Isatidis extract of the present invention.
Can also further thick paste be obtained dried Radix Isatidis extract under 50～80 ℃ of exsiccant conditions, also can be dry under reduced pressure, vacuum degree control is at 20～90kPa.Drying can be passed through water bath method, and then oven dry.
In the process of airtight to the Radix Isatidis decoction pieces in the process of extracting, merceration, alcoholic solution can the deep enough inside that is penetrated into the Radix Isatidis tissue, thereby shortened the time of hot extraction, can reduce such as the destruction to indirubin, pharmaceutically active such as indigo; Fully the effective ingredient of two kinds of opposed polarities in the Radix Isatidis at utmost can also be transferred in the alcoholic solution simultaneously owing to dissolved.It mainly is the activity of the extraction of substance in order to protect with vacuum degree control at 20～90kPa decompression recycling ethanol and concentrated medicament that the heating and refluxing extraction temperature is controlled at 50～80 ℃, avoids high temperature to the extract structural damage.
The zoopery result is as follows:
One, the refrigeration function of rabbit fever models:
1.1 animal:
Rabbit, 2.0-2.5kg (Mountain Western Medicine S University's Experimental Animal Center provides)
1.2 equipment:
DT-ITB type electronic thermometer, Shanghai Medical Instrument and Meter Factory, amount system Shanghai word 0003591
1.3 medicine and reagent:
The extract of this prepared
The aspirin sheet, pharmaceutical factory of Mountain Western Medicine S University, lot number: 20041102
The Typhoid-Paratyphoid tetravaccine is provided by Taiyuan City's epidemic prevention station
Radix Isatidis granule, Huakang Pharmaceutical Co Ltd, Shanxi produces, lot number: 20041201
2. experimental technique:
The Typhoid-Paratyphoid tetravaccine causes rabbit fever models: choose 60 of rabbit, male and female half and half are divided into 6 groups at random, 10 every group.Establish distilled water blank group respectively, aspirin 0.2g/kg positive controls, Radix Isatidis granule (crude drug) 8.0g/kg positive controls, extract (crude drug) 2.0g/kg, 4.0g/kg, a 8.0g/kg3 dosage group.During test rabbit put in the cage quiet back and surveys 3 body temperature (30min at interval), with the average of 3 body temperature as normal body temperature, by auricular vein injection Typhoid-Paratyphoid tetravaccine 1.0ml/kg, disposable immediately gavage above dosage be subjected to reagent liquid or contrast liquid.Capacity is 5.0ml/kg, surveys 1 anus temperature every 1h then, surveys altogether 5 times, and the relatively variation of body temperature after the 6 treated animal administrations the results are shown in Table 2
This technology of table 2 extract causes the refrigeration function (n=10) of rabbit fever models to the Typhoid-Paratyphoid tetravaccine
Annotate: compare with the blank group,
*P＜0.05
The result shows that rabbit is behind intravenous injection Typhoid-Paratyphoid tetravaccine, and 1h body temperature obviously raises, the 2h peaking, and later body temperature slowly descends, and recovers behind the 5h near normal.From whole process, the performance of aspirin refrigeration function is very fast, and 1h just has obvious suppression to body temperature rise after the modeling.The extract of this prepared also has tangible refrigeration function to the pyrogenicity rabbit, and shows certain dose-dependence, and the high dose group refrigeration function is particularly outstanding, although produce effects speed is slower than aspirin, antipyretic is longer action time.From showing and can see, the extract of this prepared is compared with the Radix Isatidis granule of traditional handicraft preparation, and refrigeration function obviously strengthens.
Two, the influence of xylol induced mice auricle edema
72 of male mice in kunming, body weight 18-22g (Mountain Western Medicine S University's Experimental Animal Center provides)
Medicine and reagent:
The extract of this prepared
Aspirin tablet, pharmaceutical factory of Mountain Western Medicine S University, lot number: 20041102
Radix Isatidis granule, Huakang Pharmaceutical Co Ltd, Shanxi produces, lot number: 20041201
Dimethylbenzene, Tianjin benchmark chemical reagent company limited, lot number: 20040806
72 of mices are divided into 6 groups at random, press gastric infusion of dosage shown in the table 3 or normal saline respectively, every day 1 time, continuous 7d is behind the last administration 1h, in the front and back of each Mus auris dextra two sided coatings 0.02ml caused by dimethylbenzene xylene inflammation, put to death animal behind the 4h, cut ears, get auricle with internal diameter 4mm card punch in the ears same area, precision is weighed, with left and right sides auricle weight difference value representation swelling degree, and calculate inhibitory rate of intumesce with following formula:
This technology of table 3 extract is to the influence of mice ear (X ± S)
Annotate: compare with the normal saline group,
*P＜0.05,
*P＜0.01
The result shows that the Radix Isatidis extract of this prepared can suppress the mice ear due to the dimethylbenzene.Compare with the blank group, Radix Isatidis extract has presented the inhibitory action of dose dependent to ease auricle swelling, and three dosage groups all have significant difference, and its suppression ratio reaches 25.52,43.93 and 54.49% respectively.The Radix Isatidis granule group has also obviously reduced auricle swelling degree, and suppression ratio is 23.20%, but still is lower than the low dose group of the extract of this prepared.
Three, the influence of PARA FORMALDEHYDE PRILLS(91,95) pain test
60 of male mice in kunming, male and female half and half, body weight 18-22g (Mountain Western Medicine S University's Experimental Animal Center provides)
Medicine and reagent:
The extract of this prepared
Aspirin tablet, pharmaceutical factory of Mountain Western Medicine S University, lot number: 20041102
Radix Isatidis granule, Huakang Pharmaceutical Co Ltd, Shanxi produces, lot number: 20041201
Formaldehyde, Tianjin benchmark chemical reagent company limited, lot number: 20030905
60 of mices are divided into 6 groups at random, and every group each 10, male and female are passed through gastric infusion or normal saline respectively for half and half, 6 group.Dosage is respectively: aspirin 0.2g/kg, indigowoad root granules (crude drug) 8.0g/kg, the extract of this prepared (crude drug) 2.0g/kg, 4.0g/kg, 8.0g/kg, every day 1 time, successive administration 5d.30min after the last administration, at mice left side vola subcutaneous injection 2% formaldehyde 20 μ l, the time (s) of licking metapedes with mice between the 0min-5min of injection back serve as fast pain index, and the time (s) of licking the vola with mice between the 20min-30min of injection back is the slow pain index, and calculating pain suppression ratio (%).
The influence that this technology of table 4 extract is tested mice formaldehyde pain (n=10, X ± S)
Annotate: compare with the blank group,
*P＜0.05,
*P＜0.01
The result shows that this technology extract does not obviously act on the fast pain due to the mice formaldehyde, but the slow pain due to the PARA FORMALDEHYDE PRILLS(91,95) has the obvious suppression effect, and becomes dose-dependence.Analgesic activity also obviously is better than the Radix Isatidis granule preparation during low dosage, and is consistent with the aspirin analgesic activity during high dose.
Four, to the dead protective effect of mice influenza infection
The extract of this prepared
Aciclovir injection, Shanxi Yabao Pharmaceutical Co., Ltd., lot number 20040301
Radix Isatidis granule, Huakang Pharmaceutical Co Ltd, Shanxi produces, lot number: 20041201
70 of Kunming mouses, male and female half and half, body weight 18-22g is provided by Mountain Western Medicine S University's Experimental Animal Center.
Virus, influenza A virus (A
1Prevent/97-53H in/capital
1N
1) Mountain Western Medicine S University's microbiology and the preservation of immunology teaching and research room.
Mice is divided into 7 groups (10 every group) at random: first group: the normal control group; Second group: the virus control group; The 3rd group: aciclovir injection group (100mg/kg); The 4th group: Radix Isatidis granule group (8g/kg); The 5th group: extract low dose group (2g/kg); The 6th group: dosage group (4g/kg) in the extract; The 7th group: extract high dose group (8g/kg).
All under the slight anesthesia of ether, intranasal vaccination influenza A virus 0.03ml/, its dosage is equivalent to 2-5LD to mice
50The chick embryo allantoic liquid that contains influenza virus.Each group of this technology extract, Radix Isatidis granule group and aciclovir injection are in viral infection clysmata administration in preceding 2 hours 1 time, and later 2 times/day, 0.2ml/ time, administration is 5 days altogether.Virus control group and normal control group give the equivalent distilled water by the same method and substitute.
Mouse invasion and death toll are observed and write down to continuous the observation 15 days day by day behind viral infection, calculates mortality rate, dead protective rate, average life day and prolong vital rates.
This extract of table 5 is to the dead protective effect of mice influenza infection
| Group | Number of animals (only) | Death toll (only) | Mortality rate (%) | Dead protective rate (%) | Average life day (my god) | Prolong vital rates (%) |
| 1 | 10 | 0 | 0 ** | 14.00±0.00 ** | ||
| 2 | 10 | 9 | 90 | 6.50±2.28 | ||
| 3 | 10 | 3 | 30 * | 66.7 | 12.25±4.22 ** | 88.46 |
| 4 | 10 | 8 | 70 | 22.2 | 9.16±3.30 * | 40.92 |
| 5 | 10 | 6 | 60 | 33.3 | 9.70±2.10 * | 49.23 |
| 6 | 10 | 5 | 50 | 44.4 | 10.25±4.20 ** | 57.69 |
| 7 | 10 | 4 | 40 | 55.6 | 11.04±5.61 ** | 69.85 |
Annotate: with the 2nd group of comparison,
*Be P＜0.05,
*Be P＜0.01
The extract of this prepared causes that to the mice influenza virus the average life of dead protection and prolongation animal daily has clear meaning.Its this technology extract has the death of infective virus animal protection and an average life day prolongation aspect remarkable effect is all arranged, and this effect and dosage are proportionate.Test shows that aciclovir injection is the strongest aspect the effect of resisiting influenza virus, the Radix Isatidis granule preparation that the effect of this extract is obviously strong and traditional handicraft prepares.
Description of drawings
Fig. 1 is indirubin, indigo TLC chromatogram
1 and 5 is the Radix Isatidis granule according to the decoction alcohol precipitation method preparation of Chinese Pharmacopoeia
2 and 6 is extract of the present invention
3 is that indigo reference substance 4 is the indirubin reference substance
Fig. 2 is a total amino acids TLC chromatogram
1 is that leucine 2 is glutamic acid for tyrosine 7 for extract 8 of the present invention for proline 6 for the Radix Isatidis granule 5 that the decoction alcohol precipitation method according to Chinese Pharmacopoeia prepares for arginine 4 for valine 3
The specific embodiment
Embodiment 1:
A kind of Radix Isatidis extract, it is to be made by following preparation steps:
Get Radix Isatidis decoction pieces 50 grams, place extraction pot, add 70% alcoholic solution of 8 times of weight of medical material, kept 10 ℃ of dippings of alcoholic solution 24 hours, heating keeps reflux, extract, 3 times to extraction pot, each 0.5 hour, merge the ethanol extraction drug solns, remove residue with vertical and high-speed centrifuge branch, forward in the distilling column, keep 70 ℃ by the vacuum in the evacuation control distillation column under to the distillation of ethanol extraction drug solns and reclaim ethanol, extremely the drug solns that extracts does not have till the alcohol flavor, be not steam most of moisture to there being alcoholic acid drug solns decompression under the 90kPa in vacuum again, to thick paste, can obtain product with 70 ℃ of drying with water bath concentrated solutions.Extraction pot and distilling column are the conventional utensil that sell and that pharmaceuticals industry is used of the routine on the market.The Radix Isatidis decoction pieces means that getting Radix Isatidis removes impurity, cleans, and runs through, and cuts sheet, the product after big guns such as drying are processed.
The extract that the present invention obtains can prepare medicine, as: add sweeting agent, flavoring agent, coloring agent and antiseptic, the granule for preparing oral administration according to the pharmaceutical factory common process, granule contains nontoxic acceptable correctives and the excipient that is applicable to the preparation granule, as: sucrose or sweeting agent, Herba Menthae, Borneolum Syntheticum etc.; Dextrin, starch etc.
Embodiment 2:
A kind of Radix Isatidis extract, it is to be made by following preparation steps:
Get Radix Isatidis decoction pieces 200 grams, place extraction pot, add 60% alcoholic solution of 5 times of weight of medical material, kept 20 ℃ of dippings of alcoholic solution 12 hours, heating keeps 75 ℃ of reflux, extract, 2 times to extraction pot, each 1 hour, merge the ethanol extraction drug solns, remove residue with vertical and high-speed centrifuge branch, forward in the distilling column, vacuum by evacuation control distillation column keeps 70 ℃ to the distillation of ethanol extraction drug solns and reclaim ethanol, do not have the alcohol flavor to the drug solns that extracts till, be not steam most of moisture to there being alcoholic acid drug solns decompression under the 70kPa in vacuum again, with 60 ℃ of drying with water bath concentrated solutions to thick paste, its relative density of surveying under 50 ℃ of situations is 1.30, and thick paste can obtain product in 75 ℃ of dryings.The Radix Isatidis decoction pieces means that getting Radix Isatidis removes impurity, cleans, and runs through, and cuts sheet, the product after big guns such as drying are processed.
The extract that the present invention obtains can prepare medicine, as add sweeting agent, flavoring agent, coloring agent and antiseptic, the tablet of preparation oral administration, tablet contains the active component of the nontoxic acceptable mixed with excipients that is applicable to the preparation tablet, as: inert diluent, calcium carbonate, sodium carbonate, lactose, calcium phosphate or sodium phosphate; Granulating agent and disintegrating agent are as alginic acid; Binding agent, as: starch, gelatin or arabic gum; Lubricant, as: magnesium stearate, stearic acid or Pulvis Talci.Tablet can not have sugar-coat or uses the known technology coating, delays disintegration and absorption in gastrointestinal tract, thereby continuous action is provided in a long time, can use the time-delay material, as glyceryl monostearate or distearin.
Embodiment 3:
A kind of Radix Isatidis extract, it is to be made by following preparation steps:
Get 1 kilogram of Radix Isatidis decoction pieces, place extraction pot, add 85% alcoholic solution of 15 times of weight of medical material, kept 30 ℃ of dippings of alcoholic solution 12 hours, heating keeps 60 ℃ of reflux, extract, 4 times to extraction pot, each 0.5 hour, merge the ethanol extraction drug solns, remove residue with vertical and high-speed centrifuge branch, forward in the distilling column, vacuum by evacuation control distillation column keeps 70 ℃ to distillation of ethanol extraction drug solns and recovery ethanol, till not having the alcohol flavor to the drug solns that extracts, be not steam most of moisture to there being alcoholic acid drug solns decompression under the 60kPa in vacuum again, with 50 ℃ of drying with water bath concentrated solutions to thick paste, its relative density of surveying under 50 ℃ of situations is 1.35, and thick paste is described extract.The Radix Isatidis decoction pieces means that getting Radix Isatidis removes impurity, cleans, and runs through, and cuts sheet, the product after big guns such as drying are processed.
The extract that the present invention obtains can prepare the medicine of oral liquid dosage form, as: add sweeting agent, flavoring agent, coloring agent and antiseptic, hard or soft capsule, the syrup etc. of preparation oral administration.The preparation that orally uses also can be made into hard gelatin capsule, wherein active component and inert solid diluent, as: calcium carbonate, calcium phosphate or Kaolin mix, or make Perle, wherein extract component and water or oil medium, as: Oleum Arachidis hypogaeae semen, liquid paraffin or mixed with olive oil.
Embodiment 4:
A kind of Radix Isatidis extract, it is to be made by following preparation steps:
Get 5 kilograms of Radix Isatidis decoction pieces, place extraction pot, add 70% alcoholic solution of 6 times of weight of medical material, kept 25 ℃ of dippings of alcoholic solution 24 hours, extraction pot is heated to boiling reflux to be extracted 4 times, each 0.5 hour, the drug solns that merges ethanol extraction, remove residue with vertical and high-speed centrifuge branch, forward in the distilling column, the vacuum of controlling distillation column by evacuation keeps 70 ℃ to distillation of ethanol extraction drug solns and recovery ethanol, extremely the drug solns that extracts does not have till the alcohol flavor, be not steam most of moisture to there being the decompression of alcoholic acid drug solns under the 80kPa in vacuum again, to thick paste, its relative density of surveying under 50 ℃ of situations is 1.30 with 80 ℃ of drying with water bath concentrated solutions.Thick paste can obtain dried product in 75 ℃ of dryings.The Radix Isatidis decoction pieces means that getting Radix Isatidis removes impurity, cleans, and runs through, and cuts sheet, the product after big guns such as drying are processed.
The extract that the present invention obtains can prepare medicine, as: add sweeting agent, flavoring agent, coloring agent and antiseptic, hard or soft capsule, the syrup etc. of preparation oral administration.The preparation that orally uses also can be made into hard gelatin capsule, wherein active component and inert solid diluent, as: calcium carbonate, calcium phosphate or Kaolin mix, or make Perle, wherein extract component and water or oil medium, as: Oleum Arachidis hypogaeae semen, liquid paraffin or mixed with olive oil.
Claims (7)
1. a Radix Isatidis extract is characterized in that it is to be made by following preparation steps,
A. choose the Radix Isatidis decoction pieces, add 50～85% alcoholic solution of 5～15 times of weight of decoction pieces, place container, airtight, merceration, the temperature of merceration is 10～30 ℃, the time is 1～24 hour;
B. the container heating and refluxing extraction is obtained the alcoholic solution of medicine;
C. filter,, get the aqueous solution of medicine extracting solution heating recovery ethanol;
D. medicinal liquid is evaporated to and obtains thick paste.
2. Radix Isatidis extract according to claim 1, the temperature that it is characterized in that described step a merceration is 10 ℃, the time is 24 hours.
3. Radix Isatidis extract according to claim 1 is characterized in that the operation of described step b repeats 1～4 time, and each heating and refluxing extraction time was controlled at 0.5～5 hour.
4. Radix Isatidis extract according to claim 1 is characterized in that described step c heating and temperature control at 50～80 ℃, and vacuum degree control is at 20～90kPa.
5. Radix Isatidis extract according to claim 3, the concentration of alcohol after it is characterized in that merging is 50～70%.
6. Radix Isatidis extract according to claim 1 is characterized in that the relative density that thick paste that described steps d obtains records is 1.25-1.35 in the time of 50 ℃.
7. the preparation method of a Radix Isatidis extract is characterized in that it comprises following preparation steps:
A. choose the Radix Isatidis decoction pieces, add 50～85% alcoholic solution of 5～15 times of weight of decoction pieces, place container, airtight, merceration; The temperature of merceration is 10～30 ℃, and the time is 1～24 hour;
B. the container heating and refluxing extraction is obtained the alcoholic solution of medicine; Operation repeats 1～4 time, and each heating and refluxing extraction time was controlled at 0.5～5 hour;
C. filter,, get the aqueous solution of medicine extracting solution heating recovery ethanol; Heating and temperature control is at 50～80 ℃, and vacuum degree control is at 20～90kPa;
D. medicinal liquid is evaporated to and obtains thick paste; The relative density that the thick paste that obtains records in the time of 50 ℃ is 1.25-1.35.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100127677A CN100425250C (en) | 2005-08-28 | 2005-08-28 | Isatis root extract and its preparation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100127677A CN100425250C (en) | 2005-08-28 | 2005-08-28 | Isatis root extract and its preparation method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1733042A CN1733042A (en) | 2006-02-15 |
| CN100425250C true CN100425250C (en) | 2008-10-15 |
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| CN103070931A (en) * | 2013-02-06 | 2013-05-01 | 朱柏丞 | Traditional Chinese medicine extract with heat-clearing, detoxifying, antibacterial and anti-inflammatory functions and preparations and quality control method thereof |
| CN115990201B (en) * | 2022-11-08 | 2024-03-26 | 神威药业集团有限公司 | Preparation method of qingkailing preparation |
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| CN1453033A (en) * | 2003-05-19 | 2003-11-05 | 吴梅春 | Freeze dried isatis root powder for injection and its prepn |
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| CN1453033A (en) * | 2003-05-19 | 2003-11-05 | 吴梅春 | Freeze dried isatis root powder for injection and its prepn |
Non-Patent Citations (2)
| Title |
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| 板蓝根制剂提取工艺的探讨. 陈玉生，许东明.中国中药杂志，第15卷第7期. 1990 |
| 板蓝根制剂提取工艺的探讨. 陈玉生，许东明.中国中药杂志，第15卷第7期. 1990 * |
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Assignee: Anhui Guangyintang Pharmaceutical Co., Ltd. Assignor: Zhang Zhongsu|Wang Dai|Zhao Zhengbao Contract record no.: 2010340000130 Denomination of invention: Isatis root extract and its preparation method Granted publication date: 20081015 License type: Exclusive License Open date: 20060215 Record date: 20100726 |
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