CN100413880C

CN100413880C - Derivative of protopanoxadiol, prepn. method and application thereof

Info

Publication number: CN100413880C
Application number: CNB2005100536166A
Authority: CN
Inventors: 郭洪祝; 田茵; 果德安
Original assignee: Peking University
Current assignee: Peking University
Priority date: 2005-03-09
Filing date: 2005-03-09
Publication date: 2008-08-27
Anticipated expiration: 2025-03-09
Also published as: CN1830995A

Abstract

The present invention discloses a protopanoxadiol derivative, a preparation method and an application thereof. The structural formula I of protopanaxatriol derivative provided by the present invention discloses in the specification, wherein R1 is-OH or H; R2 is-CH3 or-CH2OH; R3 is-CH3 or-CH2OH. The present invention adopts a microbial conversion technology to successfully decorate the structure of protopanoxadiol so as to obtain various new compounds. Proved by in vitro antitumor cell tests, the compounds have good antineoplastic activity and can be used as the active ingredients of antitumor medicines; the compounds have wide applications.

Description

Protopanoxadiol derivative and preparation method thereof and application

Technical field

The present invention relates to compound and preparation method thereof and application, particularly relate to protopanoxadiol derivative and preparation method thereof, and the medicinal use of this derivative.

Background technology

Malignant tumour is one of principal disease of harm humans health, and the research and development antitumor drug is the important research content of field of medicaments always.Up to now, Chang Yong main flow antitumor drug derives from natural drug mostly clinically, or is the product that lead compound obtains with the natural drug behind structure of modification.As the vincristine(VCR) of plant origin, the etoposide that podophyllotoxin is transformed, and the taxol in the fashionable world etc., this shows that natural product remains an important source of antitumor drug.

Genseng is famous Chinese medicine, has occupied consequence in traditional Chinese materia medica system.Because its determined curative effect is active various, is the focus of research always.In recent years, at discovering that main active ingredient ginsenoside in the genseng carries out, this compounds has good antitumor action, no matter is the cell in vitro test, still epidemiology survey, the result shows that all this compounds has activity antitumor and reduction tumour risk.It should be noted that the in vitro tests result shows, the protopanoxadiol effect is better than ginsenoside.But protopanoxadiol belongs to tetracyclic triterpenoid, lacks reactive group, and reaction site is less, adopts the conventional chemical reaction method to be difficult to prepare the derivative that meets the demands.

Summary of the invention

The purpose of this invention is to provide class protopanoxadiol derivative and preparation method thereof.

Protopanoxadiol derivative provided by the present invention, its structural formula are formula I,

Wherein, R ₁For-OH or H; R ₂For-CH ₃Or-CH ₂OH; R ₃For-CH ₃Or-CH ₂OH.

More specifically, protopanoxadiol derivative of the present invention is that structural formula is the compound of formula II, formula III or formula IV.

The preparation method of protopanoxadiol derivative of the present invention, comprise the steps: 1) the fermentation culture microorganism, in substratum, add protopanoxadiol, then transform cultivation, obtain fermented liquid after removing mycelium, described microorganism is little Ke Yinhan mould (Cunninghamella), Mucor (Mucor), chain lattice spore (Alternaria), colter mould (Absidia), rhodotorula (Rhodotorula), the bacterial strain of mould (Syncephalastrum) or head mold (Rhizopus) genus altogether; 2) with described fermented liquid through the extraction after, the evaporate to dryness extraction liquid obtains the conversion product residue; 3) with described conversion product residue with the silicagel column purifying, described silicagel column purifying adopts chloroform-ethyl acetate biphasic system gradient elution, collects to merge component; 4) with described component RPLC purifying, obtain product.

Wherein, microorganism is preferably mould (Syncephalastrum) or head mold (Rhizopus) genus altogether; The concentration of protopanoxadiol is 2-2000 μ g/mL in the step 1) substratum.

Another object of the present invention provides the purposes of protopanoxadiol derivative of the present invention.

The inventor confirms that by experiment protopanoxadiol derivative of the present invention has good antineoplastic activity, can be used as the activeconstituents of antitumor drug.

The activeconstituents of these antitumor drugs can be that to be selected from structural formula be formula I, particularly one or more in the compound of formula II, formula III and formula IV.

Be in the medicine of activeconstituents with the protopanoxadiol derivative, can also adding one or more pharmaceutically acceptable carriers when needing.Described carrier comprises the thinner, vehicle, weighting agent, tackiness agent, wetting agent, disintegrating agent, absorption enhancer, tensio-active agent, absorption carrier, lubricant of pharmaceutical field routine etc., all can be according to the ordinary method preparation of pharmaceutical field.

The present invention utilizes microbiological transformation technology, protopanoxadiol has successfully been carried out structural modification, obtained multiple novel cpd, confirm by the extracorporeal anti-tumor test cell line, these compounds have better antitumor activity, can be used as the activeconstituents of antitumor drug, have purposes widely.

Description of drawings

Fig. 1 is the color atlas of conversion reaction

Embodiment

Embodiment 1, structural formula are the preparation of the compound of formula II, formula III and formula IV

The present invention adopts microbial conversion process, is raw material with the protopanoxadiol, by fermentation, step such as extraction, separation, prepares The compounds of this invention.Microorganism with conversion capability comprises: the Mildy Way mould (Cunninghamella), Mucor (Mucor), chain lattice spore (Alternaria), colter mould (Absidia), rhodotorula (Rhodotorula), the microorganism of mould (Syncephalastrum) or head mold (Rhizopus) genus altogether; What wherein conversion capability was stronger is the bacterial strain that is total to mould (Syncephalastrum) and head mold (Rhizopus) genus.These bacterial strains all can be put on the solid slant culture base in 4 ℃ of refrigerators and be preserved available from Chinese Academy of Sciences microbial strains preservation administrative center (CGMCC) or Chinese food fermentation research institute industrial microorganism preservation administrative center (CICC).Fungus culture is selected potato culture for use, and microbial culture is selected the LB substratum for use.

The preparation of potato culture (PDA substratum): get 200g peeling potato, thinly slice, put into suitable quantity of water, boil back 80 ℃ of insulation 1h.With getting filtrate after the double gauze filtration, add 20g glucose, stirring is dissolved glucose fully, is settled to 1000ml with water.Preparation solid slant culture base adds 3% agar in the liquid medium within again.

The preparation of bacteria culture medium (LB substratum): every 1000mL liquid nutrient medium adds the 5.0g yeast extract, the 10.0g peptone, and 10.0g NaCl is dissolved in water adjust pH to 7.0.Preparation solid slant culture base adds 1.5% agar in the liquid medium within again.

With zhizopchin Rhizopus chinensis CICC 3043 is example, and the preparation structural formula is that the process of compound of formula II, formula III, formula IV is as follows:

1, fermentation, conversion and extraction

Zhizopchin Rhizopus chinensis CICC 3043 is inserted in 4 250mL triangular flasks (the 100mL potato culture is housed), as seed liquor.Treat that mycelial growth is in animated period in 160rpm, 25 ℃ of following shaking culture on the shaking table after 1 day,, add 20 1000mL and shake in the bottle (the 400mL potato culture is housed) with the seed liquor of aseptic pipette, extract 5mL.After the shaking culture 1 day, each shakes and adds 20mg protopanoxadiol (0.2mL, 100mg/mL MeOH solution), shared 400mg substrate in the bottle.The same terms continues down to transform 5 days, with filtering fermentation liquor, and the filtering mycelium, filtrate is used equal volume of ethyl acetate 3 times, and it is dried that extraction liquid is evaporated to, and obtains the about 1.5g of conversion product residue.

2, silicagel column purifying

With the residue obtained small amount of methanol that is dissolved in, mix with 2.5g column chromatography silica gel (200-300 order) and to mix sample, seasoning, add to the chromatogram capital that 60g silica gel (200-300 order) is housed,, collect elution fraction with chloroform-ethyl acetate system gradient elution (6: 1 → 1: 8), adopt TLC analytical procedure (silica gel g thin-layer plate, chloroform-ethyl acetate (5: 5) is launched, the spraying of 10% ethanol solution of sulfuric acid, heating colour developing) resulting similar elution fraction is merged.

3, RPLC purifying

Merge component rp-hplc analysis, purifying.Analysis condition is: chromatographic column YMC ODS-A 5 μ m, and 4.6mmI.D * 250mm (Japanese YMC company), elution system is the acetonitrile-water gradient elution, concrete proportioning is: acetonitrile-water was by (30: 70 in 30 minutes, V/V) become (100: 0, V/V), and keep 100% acetonitrile 10min; Became once more in 41 minutes acetonitrile-water (30: 70, V/V) and keep 10min.Flow velocity is 0.7ml/min.The detection wavelength is 203nm, and reference wavelength is 360nm, 25 ℃ of column temperatures, sample size 10 μ L.The color atlas of conversion reaction as shown in Figure 1

Preparation condition is half preparation with chromatographic column YMC ODS-A 5 μ m, 10mm I.D * 250mm (Japanese YMC company), and (40: 60v/v), flow velocity 1.0mL/min detects wavelength 203nm to acetonitrile-water.Obtain 3 converted products such as compound that structural formula is formula II, formula III, formula IV.

Compound I I, 12-carbonyl-7 β, 15 alpha-dihydroxy-s-20 (S)-protopanoxadiol [12-oxo-7 β, 15 α-dihydroxyl-20 (S)-protopanaxadiol], white amorphous powder:

HR-FT-ICR MS (m/z) calculated for C ₃₀H ₅₀O ₅Na[M+Na] ⁺513.3550; Found513.3545; Its ¹H-NMR reaches ¹³The C-NMR data are as shown in table 1.

Compound III, 12-carbonyl-7 beta-hydroxy-20 (S)-protopanoxadiol [12-oxo-7 β-hydroxyl-20 (S)-protopanaxadiol], white amorphous powder:

HR-FT-ICR?MS(m/z)497.3595[M+Na] ⁺(calculated?for?C ₃₀H ₅₀O ₄Na[M+Na] ⁺，497.3709)。

Compound IV, 12-carbonyl-7 β, 27-dihydroxyl-20 (S)-protopanoxadiol [12-oxo-7 β, 27-dihydroxyl-20 (S)-protopanaxadiol], white amorphous powder:

HR-FT-ICR?MS(m/z)513.3548[M+Na] ⁺(calculated?for?C ₃₀H ₅₀O ₅Na[M+Na] ⁺，513.3550)。

Compound III and compound IV ¹H-NMR reaches ¹³The C-NMR data are as shown in table 2.

The hydrogen spectrum of table 1. Compound I I and carbon spectrum data (C ₅D ₅N)

The hydrogen spectrum of table 2. Compound I I and compound IV and carbon spectrum data

Above result shows that the gained compound structure is correct.

Utilize the microorganism of aforementioned other genus, concrete as racemose gongtou mould Syncephalastrum racemosum AS3.264, the mould Absidia coerulea of blue colter AS 3.3389, mucor spinosus Mucor spinosus AS 3.3450, all can adopt with last identical process to prepare Compound I I, III and IV.

The anti-tumor activity of embodiment 2, The compounds of this invention

1, experiment material

Instrument and reagent: microplate reader is a TECAN porous spectrophotometer (U.S.A); CO ₂The gas incubator is BB5060 type ultraviolet clean CO ₂Incubator (Heraeus Co.); High speed freezing centrifuge is TGL-16G high speed freezing centrifuge (a Shanghai scientific instrument factory).Used chemical reagent is analytical pure (Beijing chemical reagent factory); Cell culture medium respectively available from Sigma Chemical company (St.Louis, MO, USA) and Hyclone company (Logan, Utah, USA)

Tumor cell line: HL-60 is used in test, and human leukemia cell (human leukemia cells) purchases in Tumour Inst., Chinese Medical Academy.

Specimen: protopanoxadiol (PD) and embodiment 1 synthesize the Compound I I-IV that obtains, purity is more than 90%; Simultaneously, choose the positive control drug of cis-platinum, each compound is all with DMSO dissolving back dilution.

2, experimental technique

Adopt mtt assay to measure the half inhibiting rate IC of each test-compound to tumor cell line ₅₀Value: the tumour cell in the vegetative period of taking the logarithm is made into 2 * 10 with the RPM1640 nutrient solution that contains 10% calf serum ⁴Cell suspension, be inoculated in 96 well culture plates, at 37 ℃, 5%CO ₂, after 95% humidity condition down cultivates 24h, add the given the test agent of different concns, making final concentration is 0.1-100 μ M, continues to cultivate 72h again.Press mtt assay 570nm on microplate reader and measure absorption value.Each concentration is established three parallel holes.

3, experimental result

According to the mtt assay test result, calculate protopanoxadiol (PD) and The compounds of this invention II-IV IC to the HL-60 cell ₅₀Value, the result is as shown in table 3.

Table 3. specimen cell in vitro cytotoxic activity The selection result

The result shows that Compound I I-IV of the present invention has good antineoplastic activity, can be used as the activeconstituents of antitumor drug.

Claims

1. structural formula is the protopanoxadiol derivative of formula II, formula III or formula IV,

2. the preparation method of the described protopanoxadiol derivative of claim 1, comprise the steps: 1) the fermentation culture microorganism, in substratum, add protopanoxadiol, then transform cultivation, obtain fermented liquid after removing mycelium, described microorganism is that little Ke Yinhan is mould, Mucor, chain lattice spore, colter is mould, rhodotorula, the bacterial strain of altogether mould or Rhizopus; 2) with described fermented liquid through the extraction after, the evaporate to dryness extraction liquid obtains the conversion product residue; 3) with described conversion product residue with the silicagel column purifying, described silicagel column purifying adopts chloroform-ethyl acetate biphasic system gradient elution, collects to merge component; 4) with described component RPLC purifying, obtaining structural formula is the compound product of formula II, formula III or formula IV.

3. preparation method according to claim 2 is characterized in that: described microorganism is the bacterial strain of mould or Rhizopus altogether.

4. according to claim 2 or 3 described preparation methods, it is characterized in that: the concentration of protopanoxadiol is 2-2000 μ g/mL in the described substratum of step 1).

5. be that in the protopanoxadiol derivative of formula II, formula III or formula IV one or more are the antitumor drug of activeconstituents with the described structural formula of claim 1.

6. the application of the described protopanoxadiol derivative of claim 1 in the preparation antitumor drug.