CN100405238C - Micro-domain fluid covering-ratio control device - Google Patents
Micro-domain fluid covering-ratio control device Download PDFInfo
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- CN100405238C CN100405238C CNB200510127785XA CN200510127785A CN100405238C CN 100405238 C CN100405238 C CN 100405238C CN B200510127785X A CNB200510127785X A CN B200510127785XA CN 200510127785 A CN200510127785 A CN 200510127785A CN 100405238 C CN100405238 C CN 100405238C
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Abstract
The invention provides a control device for the covering proportion of micro-regional fluid, comprising two solution storage tanks, at least a connection pipeline, a fluid driver and two pipelines respectively connected with outside. And it utilizes the liquid level gravity of the two solution storage tanks to drive the fluid in them to simultaneously enter through the two pipelines respectively connected with outside into a reaction chamber so as to form different covering proportions of fluid in the reaction chamber. Besides, the invention assorts the fluid driver and the connection pipeline in pairs to complete liquid level transform of the two solution storage tanks so as to implement the transform of drainage area covering proportion of the reaction chamber and utilizes potential energy conservation principle to keep a constant fluid pressure in the reaction chamber in the transform course so as to reduce the interference in the reaction chamber.
Description
Technical field
The invention relates to a kind of micro-domain fluid covering-ratio control device; Particularly can keep the certain micro-domain fluid covering-ratio control device of reaction chamber fluid pressure at fluid coating ratio conversion process relevant for a kind of.
Background technology
Utilize micro-fluid chip to carry out biomedicine and detect or analyze, have the manually-operated experimental error of reduction, improve stiffness of system, reduce power consumption and amount of samples, and save advantages such as manpower and time.But because the microminiaturization of assembly, the subject matter that causes desire research is for many parameters of test block sensitivity more, as the change of pressure, temperature, humidity, drug concentration or even drug flow etc.The change of these parameters is enough to produce interference and error experimentally for little biochemical test, cause the erroneous judgement to experiment.Consult shown in Figure 1, people such as Du Li (Doring et al.) have delivered one piece of article " the thermoelectric cantilever design (Micromachined thermoelectricallydriven cantilever structures for fluid jet deflection) that drives of microcomputer that is used for deviation fluid jet direction " in Proc.IEEE MEMS (micro electro mechanical system) symposial (Proc.IEEE Micro Electro MechanicalSystem Workshop) in 1992, the fluid flow guiding system of miniature is wherein proposed, its advantage is controlled the flow direction of fluid for the signal that can utilize electricity, and have that active element such as little valve is convenient to be used, but its shortcoming causes the raising that is difficult for reaching cost on the processing procedure then for needing extra active valve member.Consult Fig. 2 and shown in Figure 3, people such as Cheng Da professor Li Guobin deliver two pieces of articles " the pre-conflux 1xN fluid switch of microcomputer (Micromachinedprefocused 1xN flow switches for continuous sampleinjection) that is used for serial sample injection " and (see the 567th page of microcomputer engineering periodical calendar year 2001 o. 11th (J.Micromechanics and Microengineering, 11,567,2001)) reach " the pre-conflux MxN fluid switch of microcomputer (Micromachined prefocused NxM flow switches for continuoussample injection) that is used for serial sample injection " and (see the 654th page of (J.Micromechanics and Microengineering of microcomputer engineering periodical calendar year 2001 o. 11th, 11,654,2001)), a kind of method of controlling direction of flow has wherein been proposed, its advantage can be controlled direction of flow very accurately for utilizing fluid dynamics, and does not need any valve member.People such as Ou Sika (Otsuka et al.) have delivered a kind of gatherer (ginseng μ-TAS that utilizes the conversion principle of flow velocity as cell, 1,30,2004), can do the action of collection and not need valve member for two kinds of cells, but its shortcoming then same pollution problems arranged easily.
In traditional biochemical field, if desire to do biochemical test, be all next operation become because of, even for to do various experiment simultaneously, still exist different changes because of fix and identical environment because external environment is difficult to keep one.Such as concentration of nutrient discarded object or the like in temperature, pressure, the nutrient culture media, and test suitable considerable of required manpower and materials cost, but still wish can be in a large number and carry out various experiment fast in the biochemical pharmacy industry.Therefore if can develop a system platform and can do two or more experiments simultaneously, but but the environment of its external biochemistry is mutually the same, with checking medicine effective platform whether, believe it is following star's product of dark tool market potential.
Summary of the invention
Fundamental purpose of the present invention provides a kind of micro-domain fluid covering-ratio control device, it makes the conversion that two kinds of fluids can be in a conversion zone be reached different coating ratios with stable manner very, to reduce the interference that fluid pressure disturbances produces target cell.
Another object of the present invention provides a kind of micro-domain fluid covering-ratio control device, be can general precision actuator can reach the control of high precision fluid coating ratio, and can control the two-way of microchannel inner fluid by sequencing and move the accurate coating ratio in control reaction zone basin.
Another purpose of the present invention provides a kind of micro-domain fluid covering-ratio control device, to be applied to cellular incubation, cell to drug test or biochemistry detection etc.
According to above-described purpose, the invention provides a kind of micro-domain fluid covering-ratio control device, it is characterized in that it comprises:
Two solution accumulator tanks are that the first fluid and second fluid are housed respectively;
At least one contact pipeline is to be communicated between aforementioned two solution accumulator tanks, and the length of aforementioned contact pipeline is enough to prevent that this first fluid and this second fluid from mixing mutually;
One fluid driver is to be incorporated into aforementioned contact pipeline, to control this first fluid and this second fluid in the moving direction of aforementioned contact pipeline; And
Two connect external pipeline, are communicated in aforementioned two solution accumulator tanks respectively, enter a reaction chamber by these two solution accumulator tank liquid level segregation drive simultaneously so that this first fluid and this second fluid connect external pipeline via aforementioned two.
Wherein aforesaid contact pipeline is a crooked pipeline.
Wherein aforesaid contact pipeline is a multiple crooked microchannel.
Wherein be in the moving direction of this contact pipeline, to finish the liquid level conversion of these two solution accumulator tanks by this first fluid of aforesaid fluid driver control and this second fluid.
Wherein be liquid level conversion by these two solution accumulator tanks, with this first fluid of conversion and this second fluid in the basin of previous reaction chamber coating ratio.
Wherein be the liquid level lifting simultaneously of controlling aforementioned two solution accumulator tanks by this fluid driver, to keep the pressure equilibrium in this reaction chamber.
Wherein above-mentioned reaction chamber is a biological reaction chamber.
Wherein above-mentioned reaction chamber is a micro-fluid chip.
The present invention be utilize the characteristic of microchannel and can rank idea, make the interference that can do two groups of above experiments and the successful non-controlling factor generation of reduction in the reaction chamber simultaneously.System and device of the present invention is simple, cost of manufacture is cheap, the working flow rate scope is big, and dark tool development potentiality can be applicable in any field that needs miniflow.
Description of drawings
For further specifying concrete technology contents of the present invention, below in conjunction with embodiment and accompanying drawing describes in detail as after, wherein:
Fig. 1 is the structural representation of traditional miniature flow guide system;
Fig. 2 is the pre-conflux 1xN fluid switch of the microcomputer structural representation that becomes known for serial sample injection;
Fig. 3 is the pre-conflux MxN fluid switch of the microcomputer structural representation that becomes known for serial sample injection;
Fig. 4 knows the cell harvestor that utilizes flow velocity conversion principle the sixth of the twelve Earthly Branches;
Fig. 5 is the structural representation in conjunction with a preferred embodiment of the micro-domain fluid covering-ratio control device of the present invention of a biological reactor;
Fig. 6 A and Fig. 6 B are the micro-domain fluid covering-ratio control device part synoptic diagram of the present invention that shows that respectively different fluid flows to; And
Fig. 7 illustrates the present invention to utilize the potential energy conservation principle in the fluid level conversion process.
Embodiment
The invention provides a kind of micro-domain fluid covering-ratio control device, it makes the conversion that two kinds of fluids can be in a conversion zone be reached different coating ratios with stable manner very, and the actuator that only needs general precision can be achieved the control of high precision coating ratio, and can control the two-way of microchannel inner fluid by sequencing and move, with the accurate coating ratio in control reaction zone basin.Apparatus of the present invention comprise at least two solution accumulator tanks, interconnect with at least one contact pipeline between each groove, and the aforementioned contact pipeline and the fluid driver of arranging in pairs or groups, each solution accumulator tank are provided with one in addition and connect external pipeline connection bio-reactor.The present invention by fluid driver with control fluid in the contact pipeline moving direction, and then control fluid potential difference between aforementioned solution accumulator tank, again by the liquid level segregation drive fluid of each solution accumulator tank respectively via the aforementioned external pipeline that connects that is communicated with, to enter simultaneously in the aforementioned bio-reactor.Owing to flow resistance increases along with getting in touch with duct length, therefore can utilize elongated microchannel to increase its flow resistance, and can relax the pressure disturbance that produces by fluid driver, again because the characteristic of microfluid, be difficult for when fluid moves in long and narrow pipeline mixing mutually, make fluid in flow process, be maintained its relative position and reach the function that it does not mix mutually.Moreover, apparatus of the present invention are for example finished the liquid level conversion of aforementioned solution accumulator tank in gravity group Pu with contact pipeline collocation fluid driver, connect outer biological reactor stream territory coating ratio conversion to reach, and keep the liquid level total potential to fix when making aforementioned solution accumulator tank liquid level conversion, make that still keeping hydrodynamic pressure in the liquid level conversion process fixes, make the bio-reactor fluid pressure keep balance, and allow the active somatic cell in the bio-reactor can not feel that when conversion fluid coating ratio external pressure has any change, only can experience the conversion of type of fluid, and then reflect the real influence of reagent really for biofluid.
Micro-domain fluid covering-ratio control device of the present invention will be described in detail as follows by following specific embodiment conjunction with figs..
Fig. 5 is the structural representation of a preferred embodiment of micro-domain fluid covering-ratio control device of the present invention in conjunction with a biological reactor.In this preferred embodiment, micro-domain fluid covering-ratio control device of the present invention is to comprise two solution accumulator tanks 11 and 12, at least one contact pipeline 13 and 14, fluid drivers 15 and two company's external pipelines 16 and 17.Aforementioned two solution accumulator tanks 11 and 12 are equipped with the first fluid 18 and second fluid 19 respectively, and are to be connected with contact pipeline 13 and 14 between aforementioned two solution accumulator tanks 11 and 12. Aforementioned contact pipeline 13 and 14 making can be used polymethylmethacrylate (PMMA) substrate, form multiple crooked microtube structure in milling machine Precision Machining mode on this substrate, for doing contact pipeline 13 and 14.
Consult shown in Fig. 6 A and Fig. 6 B, mat aforesaid fluid driver 15 of the present invention, for example gravity is helped the Pu, the control first fluid 18 and second fluid 19 can two-wayly move in aforementioned contact pipeline 13 and 14, shown in arrow A and arrow B, with conversion first fluid 18 and second fluid 19 liquid level respectively at aforementioned solution accumulator tank 11 and 12, and then change first fluid 18 and second fluid 19 in the aforementioned flow velocity that connects external pipeline 16 and 17, with conversion first fluid 18 and second fluid 19 in the basin of reaction zone 22 coating ratio.
See also Fig. 5 and Fig. 7 again, the present invention utilizes first fluid 18 and the liquid level total potential of second fluid 19 in aforementioned solution accumulator tank 11 and 12 all to keep definite value (ha+hb=ha '+hb ') in the liquid level conversion process, just control the liquid level lifting simultaneously of the first fluid 18 and second fluid 19, fluid general pressure in the bio-reactor 20 is maintained fixed in the liquid level conversion process, make cell in of the change of reactor enteroception less than extrinsic parameter, interference with abundant reduction biochemical test, make and to do various experiment and experimental result can be filled the parameter that part is reflected its reply fully at subject matter, and fluid is still kept order and is not mixed mutually in flow process, and the streamline held stationary that remains unchanged, and can utilize the rotating speed of computer control fluid driver 15 and turn to, with the coating ratio of sequencing control different fluid.Eliminate by the disturbance that design of the present invention can be produced fluid driver 15, so the actuator of available general precision (peristaltic pump) can be finished the coating ratio control of high-precision basin.
The present invention be directed to the property of micro-fluid chip, the stable dynamic diversion function of microstructure is provided, micro-domain fluid covering-ratio control device of the present invention has the following advantages: fluid can have stable flow velocity, utilize the actuator of general precision can achieve the control of high precision micro-domain fluid coating ratio and can get in touch with the two-way mobile of pipeline inner fluid by sequencing control, to obtain the accurate coating ratio in reaction zone basin.
Biomedical sector is being done the cell drug test or the exploitation of new drug needs control group simultaneously, and preferably do not have other disturbing factor to influence experimental result except controlled variable between two groups, micro-domain fluid covering-ratio control device of the present invention can meet these requirements.Moreover apparatus of the present invention are simple in structure, and are cheap for manufacturing cost, are suitable for a large amount of productions, and are easy to carry about with one, so dark tool practicality of apparatus of the present invention and huge business opportunity can be widely used in the biomedical sector.
The above is specific embodiments of the invention only, is not in order to limit claim of the present invention; All other do not break away from the equivalence of being finished under the disclosed spirit and changes or modification, all should be included in the following claim.
Claims (8)
1. micro-domain fluid covering-ratio control device is characterized in that it comprises:
Two solution accumulator tanks are that the first fluid and second fluid are housed respectively;
At least one contact pipeline is to be communicated between aforementioned two solution accumulator tanks, and the length of aforementioned contact pipeline is enough to prevent that this first fluid and this second fluid from mixing mutually;
One fluid driver is to be incorporated into aforementioned contact pipeline, to control this first fluid and this second fluid in the moving direction of aforementioned contact pipeline; And
Two connect external pipeline, are communicated in aforementioned two solution accumulator tanks respectively, enter a reaction chamber by these two solution accumulator tank liquid level segregation drive simultaneously so that this first fluid and this second fluid connect external pipeline via aforementioned two.
2. micro-domain fluid covering-ratio control device as claimed in claim 1 is characterized in that, wherein aforesaid contact pipeline is a crooked pipeline.
3. micro-domain fluid covering-ratio control device as claimed in claim 2 is characterized in that, wherein aforesaid contact pipeline is a multiple crooked microchannel.
4. micro-domain fluid covering-ratio control device as claimed in claim 1, it is characterized in that, wherein be in the moving direction of this contact pipeline, to finish the liquid level conversion of these two solution accumulator tanks by this first fluid of aforesaid fluid driver control and this second fluid.
5. micro-domain fluid covering-ratio control device as claimed in claim 4 is characterized in that, wherein is the liquid level conversion by these two solution accumulator tanks, with this first fluid of conversion and this second fluid in the basin of previous reaction chamber coating ratio.
6. micro-domain fluid covering-ratio control device as claimed in claim 1 is characterized in that, wherein is the liquid level lifting simultaneously of being controlled aforementioned two solution accumulator tanks by this fluid driver, to keep the pressure equilibrium in this reaction chamber.
7. micro-domain fluid covering-ratio control device as claimed in claim 1 is characterized in that, wherein above-mentioned reaction chamber is a biological reaction chamber.
8. micro-domain fluid covering-ratio control device as claimed in claim 1 is characterized in that, wherein above-mentioned reaction chamber is a micro-fluid chip.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB200510127785XA CN100405238C (en) | 2005-12-06 | 2005-12-06 | Micro-domain fluid covering-ratio control device |
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| CNB200510127785XA CN100405238C (en) | 2005-12-06 | 2005-12-06 | Micro-domain fluid covering-ratio control device |
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| CN1979358A CN1979358A (en) | 2007-06-13 |
| CN100405238C true CN100405238C (en) | 2008-07-23 |
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| CNB200510127785XA Expired - Fee Related CN100405238C (en) | 2005-12-06 | 2005-12-06 | Micro-domain fluid covering-ratio control device |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN86105523A (en) * | 1985-07-31 | 1987-03-18 | 冷水煤汽化规划公司 | The method and the device thereof of control fluid mixture ratio |
| US4832499A (en) * | 1985-12-06 | 1989-05-23 | Afros S.P.A | Apparatus for feeding and metering fluid components to a high pressure mixing head |
| CN1413904A (en) * | 2002-11-22 | 2003-04-30 | 西安理工大学 | Constant-pressure liquid feeding device |
| US6695017B1 (en) * | 1999-03-29 | 2004-02-24 | Steag Hamatech Ag | Method and apparatus for filling a pressure tank with a fluid |
-
2005
- 2005-12-06 CN CNB200510127785XA patent/CN100405238C/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN86105523A (en) * | 1985-07-31 | 1987-03-18 | 冷水煤汽化规划公司 | The method and the device thereof of control fluid mixture ratio |
| US4832499A (en) * | 1985-12-06 | 1989-05-23 | Afros S.P.A | Apparatus for feeding and metering fluid components to a high pressure mixing head |
| US6695017B1 (en) * | 1999-03-29 | 2004-02-24 | Steag Hamatech Ag | Method and apparatus for filling a pressure tank with a fluid |
| CN1413904A (en) * | 2002-11-22 | 2003-04-30 | 西安理工大学 | Constant-pressure liquid feeding device |
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| CN1979358A (en) | 2007-06-13 |
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