CN1003940B - Purificn. and utilization of distiller's grains - Google Patents
Purificn. and utilization of distiller's grains Download PDFInfo
- Publication number
- CN1003940B CN1003940B CN85104057.8A CN85104057A CN1003940B CN 1003940 B CN1003940 B CN 1003940B CN 85104057 A CN85104057 A CN 85104057A CN 1003940 B CN1003940 B CN 1003940B
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- fermentation
- lees
- vinasse
- present
- supernatant fluid
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Abstract
The present invention relates to a method for purifying and using liquid of waste residue, namely lees of wine industry, particularly lees which contain high cellulose. The present invention is mainly characterized in that after pH of lees is adjusted, submerged fermentation is carried out by inoculating edible fungi. After the fermentation, thalli are used for cultivating the edible fungi, or used for extracting amino acid or used as a medicine source. Supernatant fluid obtained through the fermentation is inoculated with feed yeast, and secondary submerged fermentation is carried out. After the secondary fermentation, the thalli are used as feed additives, supernatant fluid obtained through the secondary fermentation is used for cultivating algae. Fronds are used as the feed additives. COD of the rest supernatant fluid is lower than 200 milligrams per liter and is up to national standard. The method not only solves pollution in the lees, but also makes full use of nutrient substances in the lees. The present invention has the advantages of simple process, low energy consumption and high economic benefit.
Description
The present invention relates to a kind of waste residue liquid-vinasse that utilize alcoholic beverage industry, the vinasse cultivation bacterium and the algae that particularly contain high-cellulose make it change into the method for useful matteies such as medicine, food and feed.
At present, because wine brewing and alcohol industry vinasse and waste liquid serious environment pollution must be handled it, and obtain certain progress.For example, russian patent has provided for No. 614784 alcohol has been carried out one time fermentation with fodder yeast, and leavened prod is as animal feedstuff additive.In addition, the direct utilization of vinasse done to make feed after feed or the drying, referring to " sci-tech consultation ", 1985,3 phases.But last two kinds of methods can not make full use of remaining more Mierocrystalline cellulose in the vinasse, all can not digest and assimilate cellulosic feed except that ruminating animal.Also have the anaerobism biogas fermentation, not only facility investment is more, also has the secondary pollution problem, there are biological methods such as biological filter process to carry out bio-oxidation and decomposition again to waste dregs liquid, thereby the reduction biological oxygen demand (BOD), but that its shortcoming is a floor space is big, and scrubbing is not thorough, unhygienic.Because vinasse are high concentrated organic waste liquids, its organic oxygen-consumption (COD) reaches 20,000～50,000, it is dealt carefully with and is used have very big value.
Purpose of the present invention provides and a kind ofly can make full use of nutritive substance in the vinasse, therefrom transforms and extract edible fungi and other useful matter, thereby removes dirt, reaches preferable economic benefit again.
The inventive method is mainly based on following consideration: vinasse are high concentrated organic wastewaters, measure very huge, be difficult for utilizing, contaminate environment, but itself is nontoxic, is rich in a large number of nutrients, mainly be materials such as Mierocrystalline cellulose, hemicellulose and inorganic salt, VITAMIN, though be difficult for for general animal or green plants directly utilize, owing to Mycophyta (especially edible mushrooms) has the ability of very strong decomposition of cellulose and hemicellulose, it can utilize enzyme-Mierocrystalline cellulose and hemicellulose are converted into
Class, after be used again.Though the acidity of vinasse itself is not suitable for edible fungi growth, be exactly good liquid nutrient medium behind the adjusting pH.Therefore cultivate edible fungus species with it, handle vinasse, make it change into useful mycelium, reach nutritious supernatant liquor with tank fermentation method.The present invention mainly comprises the processing of vinasse: earlier vinasse are carried out adjust pH and handle, promptly add alkaloids the pH value is transferred to 5-7.As: can in vinasse, add 0.5～1%(weight percent) CaCO
3After, regulate pH value to 5～7 with NaOH solution again, also can only regulate pH with the lime class, inoculate edible mushrooms (as edible mushroomss such as needle mushroom, Pleurotus sajor-cajus) and carry out submerged fermentation-in the liquid nutrient medium of container the aerobe fermentation culture, feed sterile air, constantly stir, make microorganism fully contact oxygen, breeding rapidly, and accumulate a large amount of meta-bolitess.Fermentation back vinasse change into thalline and supernatant liquor, thalline separated with supernatant liquor, but centrifugation.Isolated thalline can be done female the kind and produce edible mushrooms.Contain rich in amino acid in the supernatant liquor after the fermentation, comprising human body necessary 8 seed amino acids, vitamin B group, polysaccharide and mineral substance have very big value, also can further handle and use, as cultivate algae such as orchid, edge or ferment with fodder yeast.
The invention provides a kind of method that can effectively eliminate vinasse pollution and economic utilization vinasse, utilize method of the present invention, can make the high raw material of cellulose, as the industrial residue liquid of the drying wine of sweet potato be converted into edible protein, amino acid fully, medicine is former or as the protein of action feed.According to predesigne, per 100 tons of waste residue liquids can extract 0.6 ton of feed yeast powder and 1 ton of dry mushroom filament.In addition in treating processes, the big step of power consumption such as do not have distillation, concentrate.
Embodiment:
In the vinasse of sweet potato system wine, add the 0.5-1.0%CaCO of weight
3After, regulate pH to 5-7 with NaOH solution, be placed on Erlenmeyer flask and in Autoclave, sterilized 20 minutes.Inoculate edible fungus (needle mushroom fungi or Pleurotus sajor-caju fungi) then, temperature was carried out under 23 ℃～28 ℃ submerged fermentation 24-48 hour in constant temperature oscillator, hunting speed is 95-110 time/minute, need only under normal pressure, carry out, become thalline and supernatant liquor generally speaking fully through the vinasse after the above-mentioned processing.
Thalline after the fermentation is separated with supernatant liquor.Thalline is inoculated on the solid medium, turn out edible mushrooms (needle mushroom or Pleurotus sajor-caju), in the supernatant liquor, fodder yeast is gone in inoculation, inoculum size 10%～15%, the secondary submerged fermentation was carried out 24 hours at 27 ± 1 ℃ in pH6～7 in constant temperature oscillator, oscillation number is 95-110 time/minute, and the back makes yeast separate with fermented liquid with centrifuging.
The thalline of Secondary Fermentation is separated with supernatant liquor, and thalline yeast additive capable of using as feed is a kind of high protein feed.The supernatant liquor of Secondary Fermentation gained is regulated pH to 9～10 with alkali, adds NaCl, makes solution contain NaCl1%, inoculates spiral shell and executes algae (Spirulina platensis) and cultivated 36-48 hour under 32-34 ℃ and 3000Lex illumination.Then with frond and water sepn.Frond is as feed, and COD has been lower than 200 mg/litre in the surplus water, meets discharging standards.Or supernatant liquor behind the Secondary Fermentation regulated pH to 6.8～7.2, inoculation chlorella (Chlorella), after 2 days with centrifuging with frond and water sepn, frond is as fodder additives, water is allow compliance with emission standards also.
Claims (3)
1, a kind of waste residue liquid-alcohol that utilizes alcoholic beverage industry, the method that particularly contains the vinasse of high-cellulose, it is characterized in that earlier the pH regulator of vinasse inoculation edible fungus to the 5-7 is carried out submerged fermentation,, obtain thalline and supernatant liquor after the fermentation 23 ℃～28 ℃ temperature bottom fermentations one to two day.
2, method according to claim 1, wherein said accent pH can add 0.5～1% CaCo in vinasse
3After, regulate pH with NaOH again, also can only regulate pH with the lime class.
3, method according to claim 1, wherein said edible fungus comprises needle mushroom fungi or Pleurotus sajor-caju fungi.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN85104057.8A CN1003940B (en) | 1985-05-23 | 1985-05-23 | Purificn. and utilization of distiller's grains |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN85104057.8A CN1003940B (en) | 1985-05-23 | 1985-05-23 | Purificn. and utilization of distiller's grains |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN85104057A CN85104057A (en) | 1986-11-19 |
| CN1003940B true CN1003940B (en) | 1989-04-19 |
Family
ID=4793605
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN85104057.8A Expired CN1003940B (en) | 1985-05-23 | 1985-05-23 | Purificn. and utilization of distiller's grains |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1003940B (en) |
-
1985
- 1985-05-23 CN CN85104057.8A patent/CN1003940B/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| CN85104057A (en) | 1986-11-19 |
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