CN100393868C - Rejuvenation method of acetobacter xylinum - Google Patents
Rejuvenation method of acetobacter xylinum Download PDFInfo
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- CN100393868C CN100393868C CNB2005100703652A CN200510070365A CN100393868C CN 100393868 C CN100393868 C CN 100393868C CN B2005100703652 A CNB2005100703652 A CN B2005100703652A CN 200510070365 A CN200510070365 A CN 200510070365A CN 100393868 C CN100393868 C CN 100393868C
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- acetobacter xylinum
- rejuvenation
- acetobacter
- present
- xylinum
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Abstract
The present invention discloses a rejuvenation method for acetobacter xylinum. The present invention is characterized in that various acetobacter culture media which use coconut water as main media are heated and sterilized, a rejuvenation agent is added after cooling, and the acetobacter xylinum is grafted and cultivated. The effect of rejuvenating the acetobacter xylinum can be achieved by repeating the process for many times. The rejuvenation method for acetobacter xylinum has the advantages of simple process and low cost, and enables the degradation time of the acetobacter xylinum to be prolonged in the cultivating process, and keeps 10 generations without degradation. The cultivated acetobacter xylinum keeps strong vitality, and the present invention can produce bacterial cellulose with high quality and high yield.
Description
Technical field
The present invention relates to a kind of rejuvenation method that is used to produce the acetobacter xylinum of bacteria cellulose.
Background technology
Acetobacter xylinum is cultivated in the nutrient solution that with the Sucus Cocois is main medium matter, can produce the fine bacteria cellulose, and comprising bacterial cellulose gel and Nano bacteria cellulose, bacteria cellulose is at food, pharmaceutically obtained widespread use.But acetobacter xylinum is degenerated in culturing process easily, and the acetobacter xylinum after degenerating, individuality diminishes, and shortens, and vigor reduces, and produces the Mierocrystalline cellulose ability, even has lost the cellulosic ability of producing.Just begin to degenerate after generally cultivating through the three generations aborning, separate again so again, rejuvenation, and general separation rejuvenation method was both consuming time, and unstable again, influenced the normal operation of producing, influence the output and the quality of bacteria cellulose again.
Summary of the invention
The rejuvenation method that the purpose of this invention is to provide a kind of acetobacter xylinum, it is to utilize rejuvenating agent to carry out rejuvenation in substratum, can make acetobacter xylinum prolong its degeneration time in culturing process, can make acetobacter xylinum keep stronger vitality again.
Design of the present invention is: to being that the various bacterium culture mediums of main medium matter are heated and disinfected with the Sucus Cocois, after cooling off, add rejuvenating agent, and insert acetobacter xylinum and cultivate, the process for several times of repeating can reach the effect to the acetobacter xylinum rejuvenation, and described rejuvenating agent is a kind of in ethanol, propyl alcohol, glycerine, propyl carbinol, the N.F,USP MANNITOL.
The rejuvenation method technology of acetobacter xylinum provided by the present invention is simple, cost is low, can make acetobacter xylinum prolong its degeneration time in culturing process, keeps for 10 generations do not degenerate, and make the acetobacter xylinum in the cultivation keep very strong vitality, bacteria cellulose is produced in high-quality, the high place of production.
Description of drawings
Fig. 1 is the process flow sheet of the rejuvenation method of a kind of acetobacter xylinum of the present invention.
Embodiment
Below in conjunction with embodiment the present invention is further described.
The various bacterium culture mediums that will be main medium matter with the Sucus Cocois were heated to 100-110 ℃ of disinfection 10-30 minute, be cooled to after 30-50 ℃ and add 1～5% rejuvenating agent by mass ratio, insert acetobacter xylinum and between 25-30 ℃, cultivated the effect that the repetition culturing process can reach the acetobacter xylinum rejuvenation for 2-10 time 2-5 days at 25-35 ℃.Described rejuvenating agent is a kind of in propyl alcohol, glycerine, propyl carbinol, the N.F,USP MANNITOL.
Embodiment 1
By weight ratio with Sucus Cocois 50 grams, (NH
4)
2SO
40.2 gram, MgSO
40.03 restrain, sucrose 15 grams mix the back and regulate pH to 4.5, sterilize 20 minutes for 100 ℃, are cooled to 30 ℃, add 0.5 gram n-propyl alcohol, as liquid nutrient medium, add acetobacter xylinum, cultivate 3 days for 30 ℃, cultivate repeatedly 2～10 times, can reach effect of rejuvenation.
Embodiment 2
By weight ratio with Sucus Cocois 500 grams, (NH
4)
2SO
42 grams, MgSO
40.3 restrain, sucrose 15 grams mix the back and regulate pH to 4.5, sterilize 20 minutes for 100 ℃, add 5 gram glycerine, are cooled to 30 ℃, as liquid nutrient medium, add acetobacter xylinum, cultivate 3 days for 30 ℃.Cultivate 2～10 times repeatedly and can reach effect of rejuvenation.
Embodiment 3
By weight ratio with Sucus Cocois 10 grams, (NH
4)
2SO
40.1 gram, MgSO
40.01 restrain, agar 0.2 gram mixes the back and regulates pH to 4.5, sterilizes 20 minutes for 100 ℃, is cooled to 50 ℃, adds 0.5 gram glycerine, is cooled to 30 ℃, as slant medium, adds acetobacter xylinum, cultivates 3 days for 30 ℃.Cultivate repeatedly 2～10 times, can reach effect of rejuvenation.
Claims (1)
1. the rejuvenation method of an acetobacter xylinum, it is characterized in that: the various bacterium culture mediums that will be main medium matter with the Sucus Cocois were heated to 100-110 ℃ of disinfection 10-30 minute, be cooled to after 30-50 ℃ and add 1～5% rejuvenating agent by mass ratio, insert acetobacter xylinum and between 25-30 ℃, cultivated the effect that the repetition culturing process can reach the acetobacter xylinum rejuvenation for 2-10 time 2-5 days at 25-35 ℃; Described rejuvenating agent is a kind of in ethanol, propyl alcohol, glycerine, propyl carbinol, the N.F,USP MANNITOL.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100703652A CN100393868C (en) | 2005-04-30 | 2005-04-30 | Rejuvenation method of acetobacter xylinum |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100703652A CN100393868C (en) | 2005-04-30 | 2005-04-30 | Rejuvenation method of acetobacter xylinum |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1693452A CN1693452A (en) | 2005-11-09 |
| CN100393868C true CN100393868C (en) | 2008-06-11 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2005100703652A Expired - Fee Related CN100393868C (en) | 2005-04-30 | 2005-04-30 | Rejuvenation method of acetobacter xylinum |
Country Status (1)
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| CN (1) | CN100393868C (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102168015B (en) * | 2010-07-27 | 2013-04-17 | 孙义 | Natural probiotic culture medium and probiotic preparation |
| CN102206689B (en) * | 2011-03-07 | 2013-04-24 | 海南椰国热带水果食品加工有限公司 | Method for modifying bacterial cellulose in the fermentation process |
| CN102250985B (en) * | 2011-08-05 | 2017-07-11 | 海南椰国食品有限公司 | Fermentation medium for bacterial cellulose |
| CN103146601B (en) * | 2013-02-21 | 2014-10-29 | 海南大学 | Bacillus spp culture medium with coconut water as main raw material |
| CN106148473A (en) * | 2015-03-27 | 2016-11-23 | 海南椰国食品有限公司 | A kind of high glucose medium for biology cellulose fermentation |
-
2005
- 2005-04-30 CN CNB2005100703652A patent/CN100393868C/en not_active Expired - Fee Related
Non-Patent Citations (6)
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| 啤酒酵母菌的复壮与培养研究. 杜双奎等.中国酿造，第7期. 2004 |
| 啤酒酵母菌的复壮与培养研究. 杜双奎等.中国酿造，第7期. 2004 * |
| 椰子的开发利用. 肖红等.海南大学学报自然科学版，第21卷第2期. 2003 |
| 椰子的开发利用. 肖红等.海南大学学报自然科学版，第21卷第2期. 2003 * |
| 非碳水化合物对木醋杆菌合成细菌纤维素影响规律的初探. 马霞等.中国酿造，第4期. 2003 |
| 非碳水化合物对木醋杆菌合成细菌纤维素影响规律的初探. 马霞等.中国酿造，第4期. 2003 * |
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| CN1693452A (en) | 2005-11-09 |
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