CN100393206C - Ligusticum wallichii extract for preventing rice from poisoning by acetochlor - Google Patents
Ligusticum wallichii extract for preventing rice from poisoning by acetochlor Download PDFInfo
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- CN100393206C CN100393206C CNB2005100318458A CN200510031845A CN100393206C CN 100393206 C CN100393206 C CN 100393206C CN B2005100318458 A CNB2005100318458 A CN B2005100318458A CN 200510031845 A CN200510031845 A CN 200510031845A CN 100393206 C CN100393206 C CN 100393206C
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- acetochlor
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- paddy rice
- ligusticum wallichii
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Abstract
The present invention relates to an extract from Ligusticum wallichii Franch for preventing paddy rice from being poisoned by acetochlor. An alcohol substance is used as an organic solvent for extraction, and the material, specifically Ligusticum wallichii Franch, is dried, pulverized and sieved; then, the Ligusticum wallichii Franch is added in an extraction container for back flow extraction in a water bath by adding the organic solvent, and the extract is obtained after concentration. After the extract from the Ligusticum wallichii Franch is diluted by adding water, the extract is directly sprayed onto the paddy rice or soil on which the acetochlor is used; the extract from the Ligusticum wallichii Franch can prevent the paddy rice from being poisoned by the acetochlor, the extract is safe for crops, and environment is not polluted.
Description
Invention field
The present invention relates to a kind of safener of weed killer herbicide Acetochlor, can protect paddy rice to exempt from Acetochlor harm, more specifically, the present invention relates to a kind of alcohols Rhizoma Chuanxiong extract of protecting preventing rice from poisoning by acetochlor.
Background technology
In the chloro-acetyl amine weed killer herbicide, activity of weeding with Acetochlor is higher, applied range, it is the preceding soil processing herbicide of a kind of selectivity bud efficient, wide spectrum, for preventing and kill off annual gramineous weed special efficacy is arranged, at present China successfully is applied to Acetochlor and mixture thereof the weed control of paddy field.But because paddy rice is responsive to Acetochlor, Acetochlor produces poisoning to the tender paddy rice of children easily when paddy field uses, make the application of Acetochlor in paddy field be subjected to strict restriction.Herbicide-safener is that a class can reduce the toxicity of weed killer herbicide to crop, by increasing the tolerance of sensitive crop, alleviates or eliminates the injury of weed killer herbicide to crop, has the material of special performance.It do not influence weed killer herbicide to the prerequisite of target activity under selectively cover crop exempt from the poisoning of weed killer herbicide, thereby make many herbicide applications scopes obtain enlarging.So promptly can be old kind new application prospect is provided, increase the service life, help the exploitation of novel herbicide again.Commercial at present chloro-acetyl amine herbicide-safener has benoxacor, cyometrinil, oxabetrinil, naphthalene dimethyl anhydride etc.The country of research mainly contains the U.S., Switzerland, Germany, Japan and Canadian, and states such as Russia, Hungary are also studied.China also pays special attention to this problem, but the synthetic and bioactivity research of relevant safener still is in the starting stage.And relevantly alleviate Acetochlor with the vegetalitas safener injury of paddy rice is not appeared in the newspapers as yet.The vegetalitas safener is a kind of novel safener, and it derives from plant, directly protects paddy rice to avoid the poisoning of weed killer herbicide as herbicide-safener with vegetable material or the active component that extracts from plant.
The vegetalitas safener is different from the safener of chemosynthesis, it derives from plant, Environmental compatibility is good, can not cause new pressure to environment, the vegetalitas safener is a brand-new exploration and breakthrough in the safener field, the main performance that safener had of its existing chemosynthesis, the advantage that has the chemosynthesis safener not had again simultaneously, it can not cause new pollution to environment in the whole ecological system.China's plant resources is abundant, and this is China's development vegetalitas resources advantage that safener has.
Summary of the invention
The object of the present invention is to provide a kind of vegetalitas herbicide-safener, by using this safener, on the one hand; can protect paddy rice to exempt from the poisoning of Acetochlor, on the other hand, reach the range of application that enlarges Acetochlor; reduce the weed killer herbicide consumption, improve herbicidal effect, reduce the weeding cost.
Of the present invention being characterized as: the Ligusticum wallichii that a kind of material as herbicide-safener is a plant (Ligusticumwallichii Franch) extract, said Rhizoma Chuanxiong extract is the alcohols extract of Ligusticum wallichii plant.Alcohols comprises one or more in methyl alcohol, ethanol, propyl alcohol, butanols, the amylalcohol.The method of Rhizoma Chuanxiong extract is: (1) is pulverized vegetable material is dried under 50～80 ℃ of conditions, pulverizes, and crosses 40～60 mesh sieves; (2) extraction places extractor with the Ligusticum wallichii fine powder through pulverizing, add organic solvents such as alcohols material, as ethanol, its addition is 4～10: 1 (W/W) with the ratio of fine powder weight, refluxing extraction in water-bath, keeping temperature is 80～90 ℃, the time is 6～10h, extract is concentrated into 4～10 times again, obtains Rhizoma Chuanxiong extract.
The protection paddy rice exempts from the method for Acetochlor injury, is that the protection paddy rice exempts from the murder by poisoning of Acetochlor with the safener of Rhizoma Chuanxiong extract as the weed killer herbicide Acetochlor.
Rhizoma Chuanxiong extract in 0.001～6.000g/L scope to rice safety.
Described Rhizoma Chuanxiong extract can with the agent of Acetochlor list or composite dose of composition pesticide of making of Acetochlor, and Rhizoma Chuanxiong extract and other insecticides adjuvant are made preparation.
The alcohols extract of Ligusticum wallichii directly is sprayed on the paddy rice or in the soil after diluting.
Directly be sprayed in the paddy rice or soil of using Acetochlor after containing the alcohols extract dilution of Ligusticum wallichii, or contain and be sprayed in paddy rice or the soil after the alcohols extract of described Ligusticum wallichii and the agent of Acetochlor list or composite dose are used in combination.
When Acetochlor concentration was 0.05mg/L, working concentration was 0.0313,0.0625,0.125,0.25,0.5,1.0, the Rhizoma Chuanxiong extract of 2.0g/L reaches 155.43%, 186.96%, 221.74%, 220.65%, 205.43%, 195.65%, 168.48% respectively to the facilitation of paddy rice plant height; Facilitation to paddy rice strain fresh weight reaches 142.97%, 162.50%, 173.96%, 172.40%, 167.97%, 164.58%, 151.31% respectively.
When Acetochlor concentration was 0.05mg/L, it is best that 0.125g/L Rhizoma Chuanxiong extract cover crop avoids the effect that Acetochlor poisons, and the facilitation of plant height and strain fresh weight is respectively 221.74% and 173.96%.
When Rhizoma Chuanxiong extract concentration is 0.125g/L, can protect paddy rice to exempt from the murder by poisoning that concentration is 0.025～0.80mg/L Acetochlor in various degree.
Result of the test:
(a) extraction of vegetable matter active ingredient
Earlier vegetable material is dried under 60 ℃ of conditions, pulverize, sieve, take by weighing the vegetable material fine powder then and place apparatus,Soxhlet's, add alcohols, the ethanol as 95%, refluxing extraction in 86～88 ℃ of water-baths gets Rhizoma Chuanxiong extract after concentrating, and places refrigerator stand-by.
(b) screening of safener
Adopt the earth culture method, in plastic casing, load onto the air-dry sandy loam that sieves, choose presoaking and germinating, be seeded in the plastic casing to the rice paddy seed that shows money or valuables one carries unintentionally.Put into illumination box then and cultivate, Acetochlor and plant extracts with variable concentrations behind the 3d are handled, cultivate 7d again after, measure plant height and the strain fresh weight of paddy rice.The result shows: Rhizoma Chuanxiong extract can obviously protect paddy rice to exempt from the injury of Acetochlor.
(c) Rhizoma Chuanxiong extract is to the influence of paddy growth
Adopt (b) described earth culture method to cultivate paddy rice, handle paddy rice with Rhizoma Chuanxiong extract behind the 3d, cultivate 7d again after, measure paddy rice plant height, strain fresh weight.
(d) Rhizoma Chuanxiong extract is to the antidotal effect of Acetochlor
Adopt (b) described earth culture method to cultivate paddy rice, handle paddy rice with Acetochlor and Rhizoma Chuanxiong extract behind the 3d, cultivate 7d again after, measurement paddy rice plant height, strain fresh weight.
Advantage of the present invention and good effect: detoxifying effect is obvious.A large amount of indoor bioassay show that Acetochlor easily produces paddy rice and poisons, and after Rhizoma Chuanxiong extract was handled paddy rice, the murder by poisoning of paddy rice obviously alleviated, and has tangible antidotal effect.Enlarge the range of application of Acetochlor.Using Acetochlor easily the tender paddy rice of children to be produced at paddy field poisons, after Rhizoma Chuanxiong extract is handled paddy rice, can protect paddy rice to exempt from the murder by poisoning of Acetochlor, thereby enlarge the range of application of Acetochlor.Ligusticum wallichii belongs to vegetable material, and good with Environmental compatibility, its extract is applied to paddy rice, can not cause new pollution to the whole ecological system.
Specific embodiment
Embodiment 1
The following examples are to further specify of the present invention, below are the results of indoor bioassay, but should regard the restriction of by any way range of application of the present invention being carried out as in any case.
Earlier the Ligusticum wallichii vegetable material is dried under 60 ℃ of conditions, pulverizes, cross 40 mesh sieves (aperture 0.47mm) after, preserve standby in refrigerator (0～4 ℃).Take by weighing vegetable material fine powder 20g and place the 250mL apparatus,Soxhlet's, add the ethanol of 200mL95%, refluxing extraction 8h in 86～88 ℃ of water-baths gets extract 20mL (being equivalent to dry powder 1g/mL) after concentrating, and places refrigerator stand-by.
In the plastic casing of 13cm * 8cm * 5cm, load onto the 200g air dried soil, add the 100mL running water.Rice paddy seed is used 0.1%HgCl earlier
2Sterilization 15min, 28 ℃ of following clear water soak 24h, 30 ℃ of following vernalization 12h, select seed of the same size to be sowed in the plastic casing after showing money or valuables one carries unintentionally, 15 in every box places 26 ℃ of temperature daytimes, 22 ℃ of evenings, intensity of illumination is to cultivate in the constant temperature illumination box of 6000LuX, and the continuous illumination 12h that circulates every day carries out chemicals treatment behind the 3d.
20% Acetochlor wetting powder is made into the solution of 0.05mg/L, in every box, add the 1mL soup respectively, again Rhizoma Chuanxiong extract is diluted with water to 0.0313,0.0625,0.125,0.25,0.5,1.0,2.0g/L the soup of seven concentration, spray the 1mL extract in every box, each concentration of treatment is established 3 repetitions, compare with clear water and 0.05mg/L Acetochlor, continue to place 26 ℃ of temperature daytimes after the dispenser, 22 ℃ of evenings, intensity of illumination is to cultivate in the constant temperature illumination box of 6000LuX, the continuous illumination 12h that circulates every day, 7d measures paddy rice plant height and strain fresh weight after the chemicals treatment.It is as shown in table 1 that Rhizoma Chuanxiong extract protection paddy rice exempts from the result of Acetochlor injury.This result is the working concentration with the 0.05mg/L Acetochlor, puts into the result that the Rhizoma Chuanxiong extract working concentration of various dose is obtained.
The effect of the Rhizoma Chuanxiong extract protection preventing rice from poisoning by acetochlor of table 1 variable concentrations
Annotate: in the * bracket is growth rate.
Embodiment 2
The land for growing field crops evaluating drug effect of protection preventing rice from poisoning by acetochlor is an example with the ethanol extract of Ligusticum wallichii.
Extracting method: press the ethanol extract that the described extracting method of summary of the invention (a) obtains Ligusticum wallichii
Rice varieties: train two excellent 288
Acetochlor: 90% acetochlor ec
Paddy rice is in sowing seedling on June 21st, 2004, carry out chemicals treatment when treating rice shoot length to 3 leaves, 1 heart, processing method is that rice shoot is carried out even directed spraying, every mu of dilution that sprays after 90% acetochlor ec 30mL converts water 40kg, 7d finds that tangible poisoning appears in paddy rice behind the medicine, symptom of chemical damage is the plant dwarfing, root system is undeveloped, the paddy rice plant height is starkly lower than the paddy rice plant height that is untreated with breeding time, and every mu of mixed liquid that sprays the ethanol extract 8kg of 90% acetochlor ec 30mL and Ligusticum wallichii is converted the dilution behind the water 40kg, the 7d paddy rice does not have obvious symptom of chemical damage after the dispenser, the paddy rice plant height is near being untreated with the paddy rice plant height of breeding time, and the ethanol extract that Ligusticum wallichii is described has alleviates the injury of Acetochlor to paddy rice significantly.
Claims (2)
1. method of protecting preventing rice from poisoning by acetochlor; it is characterized in that: Ligusticum wallichii alcohols extract directly is sprayed on after diluting on the paddy rice of using Acetochlor or in the soil; or with the agent of Acetochlor list or composite dose of combination of Acetochlor after be sprayed in paddy rice or the soil, the alcohols extract of Ligusticum wallichii in 0.001～6.000g/L working concentration scope to rice safety.
2. a kind of method of protecting preventing rice from poisoning by acetochlor according to claim 1 is characterized in that: described alcohols is one or more in methyl alcohol, ethanol, propyl alcohol, the amylalcohol.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100318458A CN100393206C (en) | 2005-07-11 | 2005-07-11 | Ligusticum wallichii extract for preventing rice from poisoning by acetochlor |
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2005100318458A CN100393206C (en) | 2005-07-11 | 2005-07-11 | Ligusticum wallichii extract for preventing rice from poisoning by acetochlor |
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| Publication Number | Publication Date |
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| CN1714640A CN1714640A (en) | 2006-01-04 |
| CN100393206C true CN100393206C (en) | 2008-06-11 |
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| CNB2005100318458A Expired - Fee Related CN100393206C (en) | 2005-07-11 | 2005-07-11 | Ligusticum wallichii extract for preventing rice from poisoning by acetochlor |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102283197A (en) * | 2011-08-05 | 2011-12-21 | 湖南人文科技学院 | Application of o-hydroxybenzoic acid lactone compounds to alleviation of poisoning of amide herbicides on paddy rice |
| CN105284799B (en) * | 2015-11-23 | 2018-06-26 | 湖南省农业生物技术研究中心 | A kind of plant protection product and the method for slowing down industrial hemp poisoning |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998039297A1 (en) * | 1997-03-04 | 1998-09-11 | Zeneca Limited | Compositions for safening rice against acetochlor |
| CN1249660A (en) * | 1997-03-04 | 2000-04-05 | 泽尼卡有限公司 | Compositions for safening rice against acetochlor |
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2005
- 2005-07-11 CN CNB2005100318458A patent/CN100393206C/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998039297A1 (en) * | 1997-03-04 | 1998-09-11 | Zeneca Limited | Compositions for safening rice against acetochlor |
| CN1249660A (en) * | 1997-03-04 | 2000-04-05 | 泽尼卡有限公司 | Compositions for safening rice against acetochlor |
Non-Patent Citations (2)
| Title |
|---|
| 川芎提取物的制备及其加料试验. 钟添，钟富荣，李丛民，蒋德祥，杨华连.烟草科技，第4期. 2002 |
| 川芎提取物的制备及其加料试验. 钟添，钟富荣，李丛民，蒋德祥，杨华连.烟草科技，第4期. 2002 * |
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| CN1714640A (en) | 2006-01-04 |
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Assignee: Hunan Zhennong Technology Co., Ltd. Assignor: Hunan Agricultural University Contract fulfillment period: 2008.7.14 to 2018.7.14 Contract record no.: 2009430000134 Denomination of invention: Ligusticum wallichii extract for preventing rice from poisoning by acetochlor Granted publication date: 20080611 License type: Exclusive license Record date: 20091026 |
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Free format text: EXCLUSIVE LICENSE; TIME LIMIT OF IMPLEMENTING CONTACT: 2008.7.14 TO 2018.7.14; CHANGE OF CONTRACT Name of requester: HUNAN ZHENNONG SCIENCE AND TECHNOLOGY CO., LTD. Effective date: 20091026 |
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Granted publication date: 20080611 Termination date: 20210711 |