CN100389781C - Use of pharmacy for feline ginseng volatile oil - Google Patents
Use of pharmacy for feline ginseng volatile oil Download PDFInfo
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- CN100389781C CN100389781C CNB200610052004XA CN200610052004A CN100389781C CN 100389781 C CN100389781 C CN 100389781C CN B200610052004X A CNB200610052004X A CN B200610052004XA CN 200610052004 A CN200610052004 A CN 200610052004A CN 100389781 C CN100389781 C CN 100389781C
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Abstract
An application of the volatile oil of volvate actinidia in preparing the antibacterial medicines in the form of injection, capsule, dripping pill, tablet, particle, etc is disclosed. Said volatile oil is prepared from the fresh leaves of volvate actinidia through conventional extracting.
Description
Technical field
The present invention relates to the purposes of Radix actinidiae valvatae leaf's volatile oil, especially the purposes in pharmacy.
Background technology
Along with human living standard's raising, the extensive application of antibiotics, chemicals causes some mushroom to develop immunity to drugs, and life has caused serious threat to human being's production for this.The countries in the world medical experts constantly are devoted to seek the control medicament that toxic and side effects is little, mushroom is difficult for developing immunity to drugs.China's Chinese herbal medicine resource is abundant, and high-efficiency low-toxicity is with low cost.Vast Chinese medicine worker has made a large amount of good tries to the activity of Chinese herbal medicine antibacterial and bacteriostatic, has accumulated many experiences.
The Radix actinidiae valvatae is the big seed Fructus actinidiae chinensis of Actinidiaceae actinidia Actinidia macrospermaC.F.Liang or to calyx Fructus actinidiae chinensis Actinidia.valvata Dunn.The former is commonly called as " pearls and jewels " in Chinese medicine, the latter is commonly called as " heroin ".Mainly be distributed in China East China and South China, concentrate on provinces such as Guangdong, Hunan, Hubei, Jiangxi, Zhejiang, Jiangsu, Anhui.Put down in writing according to the Chinese medicine dictionary: this flavour of a drug hardship, cold in nature, attach to the lung and stomach meridians; Rhizome is used as medicine; Has the effect that heat-clearing and toxic substances removing, expelling wind and removing dampness, detumescence go furuncle; Clinically be used for the treatment of diseases such as sores ulceration of skin abscess, leprosy, osteomyelitis, rheumatic arthralgia, leucorrhea abnormal, liver cirrhosis jaundice ascites, the treatment that also is used for pulmonary carcinoma and digestive tract tumor among the people.The Radix actinidiae valvatae is suppressing tumor growth, and improving the sign of life aspect has unique effect, through repeatedly practising, has formed the proved recipe based on Radix actinidiae valvatae's various treatment tumors gradually, causes the concern of Chinese medicine circle.Because the good efficacy of Radix actinidiae valvatae aspect antitumor raising immunity to such an extent as to other pharmacological action has been ignored greatly, all do not have relevant for the medicinal record of volatile oil of Radix actinidiae valvatae in pharmacopeia and the various book on Chinese herbal medicine.Up to the present, yet there are no bibliographical information about this traditional medicine volatile oil chemical constituent, pharmacological action and application thereof.Application to the Radix actinidiae valvatae, also only only limit to after its whole processing, make Chinese patent medicine jointly as one of polypharmaceutic raw material and other raw material, do not find as yet with Radix actinidiae valvatae be raw material particularly as single raw material process also/maybe with the extract that processes directly as the manufacture method and the manufactured goods thereof of drug use.
Cited literature 2:
1.. Qiu Baolin etc. Zhejiang flora (Volume Four). Zhejiang: Zhejiang science tech publishing house, 1993:177.
2.. the new medical college in Jiangsu. Chinese medicine dictionary (descending). Shanghai: Shanghai science tech publishing house, 1977:2205.
3.. Yao Gan, Wang Tieseng. the arrangement of East China Actinidia medicinal plants. Chinese crude drug, 1989,12 (2): 15.
4.. Zhejiang Department of Public Health. Zhejiang Province's Chinese medicine processing standard. Hangzhou: Zhejiang science tech publishing house, 1994:89.
5.. Jin Ruizhi, Wang Guoqiang. the Radix actinidiae valvatae treats the tympanites example and releases. Zhejiang Journal of Traditional Chinese Medicine, 1998,33 (1): 35.
6.. Ni Qinwu, Zhuge Gansu Province. the Study on Identification of Radix actinidiae valvatae and adulterant thereof. Zhejiang College Of Traditional Chinese Medicine journal, 1999,23 (5): 60.
7.. Zhou Xingzhao. Tang Fuan opinion pulmonary carcinoma card is controlled. Zhejiang College Of Traditional Chinese Medicine journal, 2000,24 (2): 45.
8.. next ordinary, Zhang Hongyan. Zhejiang area is commonly used Chinese medicine Radix actinidiae valvatae progress. Zhejiang College Of Traditional Chinese Medicine journal, 2002,26 (1): 77～78.
Summary of the invention
The object of the present invention is to provide the new purposes of Radix actinidiae valvatae leaf's active component volatile oil, i.e. purposes in pharmacy.
Characteristics of the present invention are the application of Radix actinidiae valvatae leaf's volatile oil in preparation antibacterial bacteriostatic medicine.
The present invention is specifically related to the new application of Radix actinidiae valvatae leaf's active component volatile oil in preparation antibacterial bacteriostatic folk prescription or compound medicine.
The present invention is a raw material with Radix actinidiae valvatae leaf's volatile oil, it can be developed to suitable antibacterial bacteriostatic pharmaceutical preparation as acceptable carrier or pharmaceutic adjuvant on active component and the pharmacopedics, as injection, capsule (soft capsule, hard capsule), drop pill, tablet, granule, powder, oral liquid etc.
In order to understand essence of the present invention better, with the external bacteriostatic activity research of volatile oil of Radix actinidiae valvatae and the result of acute toxicity testing its purposes in preparation antibacterial bacteriostatic medicine is described below.
One, external bacteriostatic experiment
1. experiment material and method
1.1 the bacterial strain experimental strain comprises two kinds of gram positive bacterias: gold-coloured staphylococci (Staphylococcus aureus ATCC 25923) and bacillus subtilis (Bacillus subtilis ATCC26633); Two kinds of gram negative bacterias: escherichia coli (Escherichia coli ATCC 25922) and bacillus pyocyaneus (Pseudomonas aeruginosa ATCC 27853); Three kinds of funguses: yeast (Candida albicans ATCC 10231), Aspergillus fumigatus (Aspergillus fumigatus ATCC 9142) and Sabouraudites lanosus (Microsporum canis ATCC 36299).The clinical disease performance of each strain sees Table 1.
The clinical disease performance of each strain of table 1
1.2 experimental technique adopts agar diffusion method of the paper that external bacteriostatic activity is carried out preliminary survey.Adopt micro-dilution method measure minimal inhibitory concentration (minimum inhibitory concentration, MIC) and minimal bactericidal concentration (minimum bactericidal or fungicidal concentration, MBC/MFC).
2. experimental result
The results are shown in Table 2, table 3.
Volatile oil of Radix actinidiae valvatae has certain inhibitory action to gram positive bacteria, gram negative bacteria there is strong inhibitory action (except that this general drug resistance strain of bacillus pyocyaneus), minimal inhibitory concentration MIC is 0.78～25.50 μ l/ml, minimal bactericidal concentration 1.56～50.0 μ l/ml; In the test of fungus, volatile oil of Radix actinidiae valvatae shows stronger inhibition activity, and minimal inhibitory concentration MIC is 0.78～1.56 μ l/ml, minimal bactericidal concentration 0.78～3.12 μ l/ml.
The external bacteriostatic activity preliminary survey (paper disk method) of table 2 volatile oil of Radix actinidiae valvatae
A, b
aAntibacterial circle diameter 7～13mm represents faint activity; Antibacterial circle diameter 〉=14mm represents strong active
b-expression does not have activity; Nd represents not detect.
Minimal inhibitory concentration of table 3 volatile oil of Radix actinidiae valvatae (MIC) and minimal bactericidal concentration (MBC/MFC)
(micro-dilution method)
According to the report of volatile oil of Radix actinidiae valvatae chemical composition analysis with relevant pharmacology document, we find: the linalool in the volatile oil of Radix actinidiae valvatae has obvious antibacterial activity, and analgesia, spasmolytic, antileukemie effect are arranged; The 2-hexenoic aldehyde can be as the wound disinfection agent, tool antibacterial bacteriostatic activity; In addition, chemical compounds such as phytol, alpha-terpineol, geraniol and Palmic acid also have certain bacteriostatic activity.
Two, acute toxicity testing
1. experiment material and method
1.1 animal Kunming kind white mice 18～22g, male and female half and half, the quarantine back is standby.
1.2 the experimental technique mice is divided into 5 dosage groups by the body weight equilibrium, 20 every group.Fasting be can't help water 12 hours before the experiment, according to the preliminary experiment result, each is organized dosage and is respectively 2.00ml/kg, 2.56ml/kg, 3.20ml/kg, 4.00ml/kg, 5.00ml/kg, volatile oil of Radix actinidiae valvatae is diluted to mouse stomach with salad oil, observe 4h, 24h, each observation of 48h, 72h after the administration continuously once.72h record white mice toxic reaction and death toll are with the half lethal dose LD of improvement karber's method calculating white mice
50And 95% confidence interval.
2. experimental result
Result such as table 4.After measured, LD
50Value is 3.27ml/kg, the 95% credible 3.04～3.54ml/kg (P＞0.05) that is limited to.According to mensuration, 1ml volatile oil heavily is 1.17259 g, so LD
50Value is 3834mg/kg, the 95% credible 3565～4150mg/kg that is limited to; Press acute toxicity grading criteria and judge the true border of this sample non-toxic type.
Table 4 volatile oil of Radix actinidiae valvatae is to the influence of acute toxicity test in mice mortality rate
| Dosage (ml/kg) | Total mice | The 72h death toll | Mortality rate (%) |
| 5.00 | 20 | 20 | 100 |
| 4.00 | 20 | 15 | 75 |
| 3.20 | 20 | 10 | 50 |
| 2.56 | 20 | 3 | 15 |
| 2.00 | 20 | 0 | 0 |
In sum, volatile oil of Radix actinidiae valvatae has good efficacy in external antibacterial especially the inhibition aspect the fungus, and toxic and side effects is little, and the excellent development application prospect is arranged.
The used raw material of volatile oil of Radix actinidiae valvatae of the present invention is the fresh leaf of Radix actinidiae valvatae, but not rhizome, this will alleviate the insufficient phenomenon of Radix actinidiae valvatae's raw medicinal herbs supply to a certain extent, improve Radix actinidiae valvatae's effective rate of utilization.Method technology of the present invention is simple, meets the requirement of Chinese Pharmacopoeia, need not special installation.Through pharmacological research, find that it has tangible bacteriostatic activity, especially to the inhibitory action (through experiment confirm, volatile oil of Radix actinidiae valvatae antibacterial minimal inhibitory concentration MIC is 0.78～25.50 μ l/ml, and fungus minimal inhibitory concentration MIC is 0.78～1.56 μ l/ml) of fungus.And volatile oil of Radix actinidiae valvatae has the little advantage of toxic and side effects, the excellent development application prospect is arranged, can with other medicinal herb components compatibilities, or separately directly as medicine, be developed further into effective antibacterial new drug, make Radix actinidiae valvatae's medicinal ingredient be fully utilized, thereby filled up the blank that China's Chinese herbal medicine utilizes, have practical value.
The method that volatile oil of Radix actinidiae valvatae among the present invention adopts common steam distillation, solvent extraction method or supercritical extraction etc. to be suitable for suitability for industrialized production is prepared.The volatile oil of Radix actinidiae valvatae that obtains is faint yellow, mainly contain linalool (Linalool), 1,2-dimethyl-2,3-indoline (1,2-dimethyl, Lindoline), methyl linolenate (Linolenic acid, methylester), phytol (Phytol), linolenic acid (α-Linolenic acid), 2-hexenoic aldehyde (2-Hexenal), alpha-terpineol (α-Terpineol), geraniol ((E)-3,7-dimethyl--2,6-Octadien-l-ol) and Palmic acid chemical compounds such as (n-Hexadecanoic acid).Oil content is 0.10～0.25%, and promptly every 100g crude drug contains volatile oil 0.1～0.25g.
Above-mentioned steam distillation separates volatile oil by oil water separator, the steps include: to calculate by weight, take by weighing 0.1～20 part of Radix actinidiae valvatae leaf, add 0.5～200 part in water, vapor distillation 3～5 hours, temperature is controlled at 80～105 ℃ (distillation temperature in early stage can be provided with highlyer, and the later stage boils for a short time as long as keep), carries out oil-water separation; With oil water separator separate moisture volatile oil of Radix actinidiae valvatae; After the extracted with diethyl ether, water is removed in lyophilization (20 ℃ of following lyophilizations are more than 24 hours) or anhydrous sodium sulfate drying (more than 24 hours), filtration, concentrates and obtains volatile oil of Radix actinidiae valvatae.
Above-mentioned solvent extraction method, the steps include: to get 0.1～20 part of Radix actinidiae valvatae leaf, add organic solvent-normal hexane or 0.6～120 part of weight portion of cyclohexane extraction, carry out reflux, extract,, temperature is controlled at 60～70 ℃, refluxes 2～3 hours, when treating that the backflow temperature is reduced to room temperature, emit backflow, reclaim organic solvent, can obtain the volatile oil crude product; Add dehydrated alcohol 0.01～0.05 weight portion, fully mix, place in-20 ℃ of refrigerator-freezers and place more than 24 hours, turbid solution is filtered wax removing with the volatile oil crude product; Continue to place in the refrigerator-freezer and repeat wax removing process, clarify up to liquid; The ethanol liquid that will contain volatile oil concentrates, and promptly gets volatile oil of Radix actinidiae valvatae.
Above-mentioned supercritical extraction the steps include: to get the Radix actinidiae valvatae leaf and adds extraction kettle, extraction kettle pressure 10～50Mpa, and 20～70 ℃ of temperature are carried out supercritical CO
2Extraction, the extraction time is 0.5～15 hour, the CO that comes out from extraction kettle
2Enter piece-rate system, 30～50 ℃ of flash trapping stage temperature, pressure 10～15Mpa; 20～40 ℃ of secondary separation temperatures, pressure 4～7Mpa obtains volatile oil of Radix actinidiae valvatae, CO
2Flow velocity is 15～25 liters/h, the CO that separates
2Recycling with refrigeration system pressurization back after filtration again.
The specific embodiment
Following example is in order to further specifying the present invention, but do not limit the scope of the invention thus.
Embodiment 1:
Get the bright leaf 100g of Radix actinidiae valvatae, add 10 times of water gagings, vapor distillation 5 hours; Separate moisture volatile oil with oil water separator; Ether spends the night with anhydrous sodium sulfate drying after repeatedly extracting on a small quantity, and filtering and concentrating obtains volatile oil of Radix actinidiae valvatae.Volatile oil is light yellow transparent liquid sample, and special fragrant is arranged, and oil yield is 0.10%, and promptly 100g Radix actinidiae valvatae aquatic foods contain volatile oil 0.10g.
Volatile oil carries out the GC-MS analysis, result such as table 5 after adding a small amount of anhydrous alcohol solution.Chromatographic condition: quartz capillary column HP-5MS (30m * 0.25mm * 0.25 μ m), carrier gas is a helium, post flow 1ml/min.Temperature of vaporization chamber: 260 ℃, heating schedule is for to be warming up to 260 ℃ since 50 ℃ with 10 ℃/min; Mass spectrum condition: EI ionization source, ionizing energy 70eV, 230 ℃ of ion source temperatures, sweep limits: 30～500amu, sample size 1.0 μ L, split ratio 10: 1.
Table 5 volatile oil of Radix actinidiae valvatae component analysis result (steam distillation)
| The chemical compound title | Relative amount (%) | The chemical compound title | Relative amount (%) |
| 2-Hexenal 2-hexenoic aldehyde | 0.75 | Contain the N heterocyclic compound | 14.38 |
| β-Cyclocitral β-cyclocitral | 0.34 | 6,10,14-trimethyl, 2-Pentadecanone 6,10,14-trimethyl-2-15 ketone | 0.16 |
| The Nonanal aldehyde C-9 | 0.43 | Hexadecanoic acid, the methylester methyl hexadecanoate | 0.52 |
| β-Linalool β-linalool | 48.14 | Hexadecanoic acid Palmic acid | 1.32 |
| The Decanal capraldehyde | 0.12 | Linoleic acid, the methylester methyl linoleate | 0.67 |
| α-Terpinenol α-terpinol | 0.96 | Linolenic acid, the methylester methyl linolenate | 6.57 |
| 3, the 7-dimethyl-2-Octen-1-ol citronellol | 0.73 | The Phytol phytol | 5.29 |
| 3,7-dimethyl-2,6-Octadien-1-ol geraniol | 1.59 | Linolenic acid, the ethylester ethyl linolenate | 0.29 |
| 1,2-dimethyl, Lindoline 1,2-dimethyl-2,3-indoline | 7.94 | The Eicosane AI3-28404 | 0.34 |
| 4-ethenyl-2-methoxy, Phenol 4-vinyl-2-methoxyphenol | 0.61 | α-Linolenic acid alpha-linolenic acid | 0.48 |
| β-Lonene 4-(2,6,6-trimethyl-1-cyclohexene)-3-cyclobutenyl-2-ketone | 0.16 | The Heneicosane Heneicosane | 0.82 |
| The Dihydronepetalactone dihydronepetalactoneand | 0.79 | The Docosane n-docosane | 0.59 |
| The Dihydroactinidiolide dihydroactinidiolide | 0.76 | Tetracosanoid acid, methylester tetracosanoic acid methyl ester | 0.08 |
Embodiment 2:
Get Radix actinidiae valvatae's fresh leaf 100g, add normal hexane 600ml, refluxed 2 hours; Oil-containing normal hexane concentrating under reduced pressure adds a small amount of dehydrated alcohol 10ml thermosol, places and places 24 hours low temperature wax removing in-20 ℃ of refrigerator-freezers, filters; The wax removing step repeats 3 times, clarifies up to fluid; Filtered residue is molten with dehydrated alcohol slight fever again, and low temperature is placed and filtered, last merging filtrate; Concentrate oil-containing ethanol liquid, filtering and concentrating obtains volatile oil of Radix actinidiae valvatae.Volatile oil is light yellow transparent liquid sample, and special fragrant is arranged, and oil yield is 0.20%, and promptly the bright leaf of 100g Radix actinidiae valvatae contains volatile oil 0.20g.
Volatile oil carries out the GC-MS analysis, result such as table 6 after adding a small amount of anhydrous alcohol solution.Chromatographic condition: quartz capillary column HP-5MS (30m * 0.25mm * 0.25 μ m), carrier gas is a helium, post flow 1mL/min.Temperature of vaporization chamber: 260 ℃, heating schedule is for to be warming up to 260 ℃ since 50 ℃ with 10 ℃/min; Mass spectrum condition: EI ionization source, ionizing energy 70eV, 230 ℃ of ion source temperatures, sweep limits: 30～500amu, sample size 1.0 μ L, split ratio 10: 1.
Table 6 volatile oil of Radix actinidiae valvatae component analysis result (solvent extraction method)
| The chemical compound title | Relative amount (%) | The chemical compound title | Relative amount (%) |
| 2-Hexenal 2-hexenoic aldehyde | 0.01 | Dodeca-1,6-dien-12-ol, 6,10-dimethyl, 6,10-dimethyl-1,6-12 diene-12-alcohol (different) | 1.51 |
| 3-Hexen-1-ol 3-hexenol | 0.26 | The Tetradecane n-tetradecane | 0.16 |
| 2-Hexen-1-ol 2-hexenol | 0.06 | The Dihydronepetalactone dihydronepetalactoneand | 1.97 |
| 1-Hexene 1-hexene | 0.02 | The Iridomyrmecin iridomyrmecin | 0.40 |
| 3-Hexen-1-ol, acetate acetic acid 3-hexene ester | 0.03 | The Dihydroactinidiolide dihydroactinidiolide | 0.18 |
| 3-methyl, 3-Heptanol 3-methyl-3-enanthol | 0.05 | The Hexadecane Pentadecane | 0.08 |
| Benzyl Alcohol benzyl alcohol | 0.31 | 3,7,11,15-Tetramethyl-2-Hexadecen-1-ol 3,7,11,15-tetramethyl-2-hexadecylene alcohol | 0.24 |
| β-Linalool β-linalool | 0.93 | 2-Pentadecanone, 6,10,14-trimethyl 6,10,14-trimethyl-2-15 ketone | 0.04 |
| 3-Decyn-2-ol 2-hydroxyl-3-decine | 0.02 | N-Hexadecanoic acid Palmic acid | 3.46 |
| Phenylethyl Alcohol phenethanol | 0.04 | Linolenic acid, the methylester methyl linolenate | 0.31 |
| The Dodecane n-dodecane | 0.24 | The Phytol phytol | 1.64 |
| Cis-α-Terpineol alpha-terpineol | 0.18 | α-Linolenic acid alpha-linolenic acid | 60.97 |
| (R)-, 3,7-dimethyl-, 6-Octen-l-ol 3,7-dimethyl-6-matsutake alcohol (citronellol) | 0.13 | The Spinacen Squalene | 6.04 |
| 2,6-Octadien-1-ol, 3,7-dimethyl-, 3,7-dimethyl-2,6-octadienol (geraniol) | 0.52 | Vitamin E vitamin E | 2.05 |
| 1,2-dimethyl, Lindoline 1,2-dimethyl-2,3-indoline | 0.23 | τ-Sitosterol τ-sitosterol | 1.40 |
| Dodeca-1,6-dien-12-ol, 6,10-dimethyl, 6,10-dimethyl-1,6-12 diene-12-alcohol | 6.30 | α-Tocopherol vitamin E | 1.78 |
| 3-Hexadecyne 3-hexadecine | 1.41 | β-Amyrin β-Amyrin | 0.84 |
Example 3:
Get the Radix actinidiae valvatae leaf and add 350g input extraction kettle, extraction kettle pressure 20Mpa, 30 ℃ of temperature; 30 ℃ of flash trapping stage temperature, pressure 10Mpa; 20 ℃ of secondary separation temperatures, pressure 4Mpa.Supercritical fluid static immersing raw material 1 hour, equipment are stablized laggard action attitude extraction, collect product by receptor.Get volatile oil of Radix actinidiae valvatae 0.87g at last, volatile oil is light yellow transparent liquid sample, and special fragrant is arranged, and oil yield is 0.25%, and promptly the bright leaf of 100g Radix actinidiae valvatae contains volatile oil 0.25g.
Volatile oil carries out the GC-MS analysis after adding a small amount of anhydrous alcohol solution, the result is similar to example 1,2, find to contain linalool, 1 at least, 2-dimethyl-2, compositions such as 3-indoline, methyl linolenate, phytol, linolenic acid, 2-hexenoic aldehyde, alpha-terpineol, geraniol and Palmic acid.
The partial reference document that the present invention relates to:
(1)NCCLS-National Committee for Clinical Laboratory Standards，2001.Performancestandards for antimicrobial susceptibility testing：eleventh informational supplement.Document M100-S11.National Committee for Clinical Laboratory Standard，Wayne，PA，USA.
(2)Peana，A.T.，D’Aquila，P.S.，Chessa，M.L，Moretti，M.D.，Serra，G，Pippia，P.，2003.(-)-Linalool produces antinociception in two experimental models of pain.European Journal of Pharmacology 460，37-41.
(3)Chiang，L.C.，Chiang，W.，Chang，M.Y.，Ng，L.T.，Lin，C.C.，2003.Antileukemicactivity of selected natural products in Taiwan.American Journal of Chinese Medicine31，37-46.
(4)Chiang，L.C.，Ng，L.T.，Chiang，W.，Chang，M.Y.，Lin，C.C.，2003.Immunomodulatory activities of flavonoids，monoterpenoids，triterpenoids，iridoidglycosides and phenolic compounds of Plantago species.Planta Medica 69，600-604.
(5)Lis-Balchin，M.，Hart，S.，1999.Studies on the mode of action of the essential oil oflavender(Lavandula angustifolia P. Miller).Phytotherapy Research 13，540-542.
(6)Schutz，S.，Weissbecker，B.，Klein，A.，Hummel，H.E.，1997.Host plant selection ofthe Colorado potato beetle as influenced by damage induced volatiles of the potato plant.Natur-wissenschaften 84，212-217.
(7)Findlay，J.A.，Patil，A.D.，1984.Antibacterial constituents of the diatom Naviculadelognei.J Nat Prod 47(5)，815-818.
(8)Christina，K.，Martin，K.，Alison，R.，2005.Chemical composition and antibacterialactivity of the essential oil and the Gum of Pistacia lentiscus Var.chia.J.Agric.FoodChem.53(20)，7681-7685.
(9)Terry，L.A.，Joyce，D.C.，Khambay，B.P.S.，2003.Antifungal compounds in Geraldtonwaxflower tissues.Australasian Plant Pathology 32(3)，41-420.
(10)Yff,B.T.，Lindsey，K.L.，Taylor，M.B.，Erasmus，D.G.，Jager，A.K，2002.Thepharmacolofical screening of Pentanisia prunelloides and the isolation of the antibacterialcompound palmitic acid.J Ethnopharmacol 79(1)，101-107.
Claims (1)
1. the application of Radix actinidiae valvatae leaf's volatile oil in preparation antibacterial bacteriostatic medicine.
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| Title |
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| 浙江地区习用中药猫人参研究进展. 来平凡等.浙江中医学院学报，第26卷第1期. 2006 |
| 浙江地区习用中药猫人参研究进展. 来平凡等.浙江中医学院学报，第26卷第1期. 2006 * |
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