CN100387988C - Content detecting method for Ibuprofen, chlorphenamine maleate and Pseudoephedrine Hydrochloride compound preparation - Google Patents
Content detecting method for Ibuprofen, chlorphenamine maleate and Pseudoephedrine Hydrochloride compound preparation Download PDFInfo
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- CN100387988C CN100387988C CNB2006100455572A CN200610045557A CN100387988C CN 100387988 C CN100387988 C CN 100387988C CN B2006100455572 A CNB2006100455572 A CN B2006100455572A CN 200610045557 A CN200610045557 A CN 200610045557A CN 100387988 C CN100387988 C CN 100387988C
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Abstract
A content detecting method for pro-pseudo-chlorpheniramine compound preparation uses octadecyl silance bonding colloidal silica as packing agent. The mobile phase is monopotassium phosphate, methanol and triethyl-amine and the detecting wavelength is 215-225nm. Dissolve suitable pro-pseudo-chlorpheniramine preparation in the need testing solution with phosphate buffer solution under ultrasonic sound condition. Dissolve suitable ibuprofen, hydrochloric acid pseudoephedrine and chlorphenamine Maleate in the comparison solution with phosphate buffer solution under ultrasonic sound condition. Take each 10-20 mul of the need testing solution and the comparison solution accurately and inject into liquid chromatograph spectrometer and record chromatogram graph. Use peak acreage to calculate the content to ibuprofen, hydrochloric acid pseudoephedrine and chlorphenamine Maleate with the external standard method. It can confirm the content to three kind of component in compound preparation by using one mobile phase with the sententious and fast virtues to inspect the stability to the production and control the procreative quality of industrialization.
Description
Technical field
The present invention relates to a kind of detection method of content of Ibuprofen, chlorphenamine maleate and Pseudoephedrine Hydrochloride compound preparation, belong to compound medicinal formulation analyzing and testing field.
Background technology
Ibuprofen, chlorphenamine maleate and Pseudoephedrine Hydrochloride compound preparation is the compounded anti-cold medicine of being made by brufen, pseudoephedrine hydrochloride and three kinds of compositions of chlorphenamine maleate, is used for alleviating such as cold symptoms such as having a headache, generate heat, have a stuffy nose, have a running nose, sneeze.Adopt high performance liquid chromatography (HPLC) to measure the existing report of method of each component content in the compound medicine, but be can only detect a kind of composition under a kind of moving phase condition basically, test period is long, detects the cost height.Have not yet to see with a kind of moving phase condition, measure the report of three kinds of component content in the Ibuprofen, chlorphenamine maleate and Pseudoephedrine Hydrochloride compound preparation simultaneously.
Summary of the invention
The object of the present invention is to provide a kind of liquid chromatogram measuring method that under same moving phase condition, can determine three kinds of component content in the Ibuprofen, chlorphenamine maleate and Pseudoephedrine Hydrochloride compound preparation simultaneously.
Detection method of content of the present invention is realized may further comprise the steps:
(1) chromatographic condition: with octadecylsilane chemically bonded silica is filling agent; (8.16g potassium dihydrogen phosphate and 2.8g lauryl sodium sulfate are put in the 1000ml measuring bottle, are dissolved in water, and are diluted to scale, shake up)-methyl alcohol-acetonitrile-triethylamine is a moving phase with potassium dihydrogen phosphate; Detect wavelength 215nm～225nm, optimum detection wavelength 220nm;
(2) preparation of need testing solution: it is an amount of to get Ibuprofen, chlorphenamine maleate and Pseudoephedrine Hydrochloride compound preparation, porphyrize, and precision takes by weighing in right amount (being equivalent to brufen 40mg approximately), put in the 100ml measuring bottle, added the phosphate buffer ultrasonic dissolution 30 minutes, and be diluted to scale, shake up, filter, get subsequent filtrate as need testing solution;
(3) preparation of reference substance solution: other gets reference substance brufen 40mg, pseudoephedrine hydrochloride 6mg and chlorphenamine maleate 0.4mg puts in the same 100ml measuring bottle, added the phosphate buffer ultrasonic dissolution 30 minutes, and be diluted to scale, shake up, in contrast product solution;
(4) content assaying method: accurate respectively absorption need testing solution and each 10 μ l～20 μ l of reference substance solution inject liquid chromatograph, the record chromatogram; Press external standard method respectively with brufen, pseudoephedrine hydrochloride and content for Chlorphenamine Maleate in the calculated by peak area sample.
The detection method of content of Ibuprofen, chlorphenamine maleate and Pseudoephedrine Hydrochloride compound preparation of the present invention, (put in the 1000ml measuring bottle by 8.16g potassium dihydrogen phosphate and 2.8g lauryl sodium sulfate by potassium dihydrogen phosphate for the moving phase of chromatographic condition in above-mentioned (1), be dissolved in water, and be diluted to scale, shake up)-mixed solution of methyl alcohol-acetonitrile-triethylamine forms, the best proportioning of each component is that (40: 20: 40: 0.1), the pH value of moving phase was 4.0～5.0, and optimum pH value is 4.5.
The detection method of content of Ibuprofen, chlorphenamine maleate and Pseudoephedrine Hydrochloride compound preparation of the present invention, the preparation of reference substance solution specifically is in above-mentioned (3), precision takes by weighing chlorphenamine maleate reference substance 20mg and puts in the 50ml measuring bottle, add the phosphate buffer dissolving and be diluted to scale, shake up, precision is measured 1ml and is put in the 100ml measuring bottle, other precision takes by weighing reference substance brufen 40mg and pseudoephedrine hydrochloride 6mg puts in the same 100ml measuring bottle, added the phosphate buffer ultrasonic dissolution 30 minutes, and be diluted to scale, shake up, in contrast product solution.When the solution concentration of brufen, pseudoephedrine hydrochloride and chlorphenamine maleate is respectively between 348.0 μ g/ml～565.5 μ g/ml, 45.76 μ g/ml～74.36 μ g/ml and 3.552 μ g/ml～5.772 μ g/ml, the better linearity relation is all arranged, and its linearly dependent coefficient is respectively 0.9999,0.9997 and 0.9998.The recovery of brufen, pseudoephedrine hydrochloride and chlorphenamine maleate is respectively 99.63%, 99.53% and 99.28%.
The detection method of content of Ibuprofen, chlorphenamine maleate and Pseudoephedrine Hydrochloride compound preparation of the present invention, wherein each Ibuprofen, chlorphenamine maleate and Pseudoephedrine Hydrochloride compound preparation unit contains brufen (C
13H
18O
3) should be 190～210mg; Hydrochloric pseudoephedrine (C
10H
15NOHCl) should be 27.9～32.1mg; Contain chlorphenamine maleate (C
16H
19ClN
2C
4H
4O
4) should be 1.86～2.14mg; Its content limit is 90%～110% of a standard content.
The detection method of content of Ibuprofen, chlorphenamine maleate and Pseudoephedrine Hydrochloride compound preparation of the present invention is applicable to the Ibuprofen, chlorphenamine maleate and Pseudoephedrine Hydrochloride compound preparation of various formulations, as: solid pharmaceutical preparations such as tablet, capsule, granule, pill, powder, and their controlled release and sustained release preparation.
The present invention's good effect compared with the prior art is:
1. use the liquid chromatogram measuring method that to determine three kinds of component content in the Ibuprofen, chlorphenamine maleate and Pseudoephedrine Hydrochloride compound preparation with a kind of moving phase condition simultaneously.
2. the present invention is applicable to the Ibuprofen, chlorphenamine maleate and Pseudoephedrine Hydrochloride compound preparation of various formulations, as: solid pharmaceutical preparations such as tablet, capsule, granule, pill, powder, and their controlled release and sustained release preparation.
3. detection method of the present invention is accurate, simple to operation, and favorable reproducibility is highly sensitive, and is practical at work.
Embodiment
Embodiment one:
1. chromatographic condition and system suitability test:
Waters 600Pump type high performance liquid chromatograph (U.S. Waters company); Waters 996Photodiode Array detecting device (U.S. Waters company); Chromatographic column: VP-ODS post (250mm * 4.6mm; 100A); Moving phase: potassium dihydrogen phosphate (put in the 1000ml measuring bottle by 8.16g potassium dihydrogen phosphate and 2.8g lauryl sodium sulfate, be dissolved in water, and be diluted to scale, shake up)-methyl alcohol-acetonitrile-triethylamine (40: 20: 40: 0.1) be moving phase (transferring pH to 4.0～5.0) with phosphoric acid; Detect wavelength 215nm～225nm; Number of theoretical plate must not calculate by the brufen peak and is lower than 3000.Brufen, pseudoephedrine hydrochloride, each peak degree of separation of chlorphenamine maleate should be up to specification.
2. detect the selection of wavelength:
Precision takes by weighing chlorphenamine maleate reference substance 20mg and puts in the 50ml measuring bottle, add the phosphate buffer dissolving and be diluted to scale, shake up, precision is measured 1ml and is put in the 100ml measuring bottle, other precision takes by weighing reference substance brufen 40mg and pseudoephedrine hydrochloride 6mg puts in the same 100ml measuring bottle, adds phosphate buffer ultrasonic dissolution 30 minutes, and is diluted to scale, shake up, in contrast product solution.The accurate 20 μ l of absorption inject liquid chromatograph, are got the chromatogram and the ultraviolet spectrogram (attached chromatogram 1 and ultraviolet spectrum Fig. 2) of three kinds of compositions by the Waters996 diode array detector.Detect wavelength by chromatogram 1 and ultraviolet spectrum Fig. 2 test findings this product assay and elect 215nm～225nm as.Can satisfy least concentration content for Chlorphenamine Maleate mensuration in the pro-pseudo-chlorpheniramine preparation to greatest extent at 215nm～225nm wavelength place.
3. the preparation of reference substance solution
Precision takes by weighing chlorphenamine maleate reference substance 20mg and puts in the 50ml measuring bottle, add the phosphate buffer dissolving and be diluted to scale, shake up, precision is measured 1ml and is put in the 100ml measuring bottle, other precision takes by weighing reference substance brufen 40mg and pseudoephedrine hydrochloride 6mg puts in the same 100ml measuring bottle, adds phosphate buffer ultrasonic dissolution 30 minutes, and is diluted to scale, shake up, in contrast product solution.When the solution concentration of brufen, pseudoephedrine hydrochloride and chlorphenamine maleate is respectively between 348.0 μ g/ml～565.5 μ g/ml, 45.76 μ g/ml～74.36 μ g/ml and 3.552 μ g/ml～5.772 μ g/ml, the better linearity relation is all arranged, and its linearly dependent coefficient is respectively 0.9999,0.9997 and 0.9998.The recovery of brufen, pseudoephedrine hydrochloride and chlorphenamine maleate is respectively 99.63%, 99.53% and 99.28%.
4. the preparation of Ibuprofen, chlorphenamine maleate and Pseudoephedrine Hydrochloride compound preparation assay sample:
It is an amount of to get Ibuprofen, chlorphenamine maleate and Pseudoephedrine Hydrochloride compound preparation, and accurate the title decided porphyrize, precision takes by weighing in right amount (being equivalent to brufen 40mg approximately) and puts in the 100ml measuring bottle, adds phosphate buffer to scale, ultrasonic dissolution 30 minutes, filter, accurate subsequent filtrate 10～20 μ l that draw inject liquid chromatograph, the record chromatogram; Other gets reference substance brufen, pseudoephedrine hydrochloride and chlorphenamine maleate and makes reference substance solution in right amount, measures with method, presses external standard method with calculated by peak area, promptly.
Embodiment two: that quick Determination on content of Buluoweima
Get those quick 20 of Buluoweimas, accurate claim fixed, porphyrize, precision takes by weighing in right amount (being equivalent to brufen 40mg approximately) and puts in the 100ml measuring bottle, adds phosphate buffer to scale, ultrasonic dissolution 30 minutes, filtration is got subsequent filtrate as need testing solution; Other gets reference substance brufen 40mg, pseudoephedrine hydrochloride 6mg and chlorphenamine maleate 0.4mg puts in the same 100ml measuring bottle, adds phosphate buffer ultrasonic dissolution 30 minutes, and is diluted to scale, shakes up, in contrast product solution; Under following selected chromatographic condition: VP-ODS chromatographic column (250mm * 4.6mm; 100A); With potassium dihydrogen phosphate (8.16g potassium dihydrogen phosphate and 2.8g lauryl sodium sulfate are put in the 1000ml measuring bottle, are dissolved in water, and are diluted to scale, shake up)-methyl alcohol-acetonitrile-triethylamine (40: 20: 40: 0.1) be moving phase (transferring pH to 4.5) with phosphoric acid; Detect wavelength 220nm; Number of theoretical plate must not calculate by the brufen peak and is lower than 3000.Accurate respectively need testing solution and each 20 μ l injection liquid chromatograph of reference substance solution, the record chromatogram drawn; With calculated by peak area, the results are shown in Table 1 by external standard method.
That quick assay experimental result of table 1. Buluoweima
Embodiment three: the mensuration of that quick capsule content of Buluoweima
Get 20 of that quick capsules of Buluoweima, after accurate title was fixed, inclining content (must not lose capsule shells), and capsule shells is fixed with the accurate title in the clean back of little grooming; Get capsule 's content and mix, porphyrize, precision takes by weighing in right amount (being equivalent to brufen 40mg approximately) and puts in the 100ml measuring bottle, adds phosphate buffer to scale, and ultrasonic dissolution 30 minutes filters, and gets subsequent filtrate as need testing solution; Other gets reference substance brufen 40mg, pseudoephedrine hydrochloride 6mg and chlorphenamine maleate 0.4mg puts in the same 100ml measuring bottle, adds phosphate buffer ultrasonic dissolution 30 minutes, and is diluted to scale, shakes up, in contrast product solution; Under following selected chromatographic condition: VP-ODS chromatographic column (250mm * 4.6mm; 100A); With potassium dihydrogen phosphate (8.16g potassium dihydrogen phosphate and 2.8g lauryl sodium sulfate are put in the 1000ml measuring bottle, are dissolved in water, and are diluted to scale, shake up)-methyl alcohol-acetonitrile triethylamine (40: 20: 40: 0.1) be moving phase (transferring pH to 4.5) with phosphoric acid; Detect wavelength 220nm; Number of theoretical plate must not calculate by the brufen peak and is lower than 3000.Accurate respectively need testing solution and each 20 μ l injection liquid chromatograph of reference substance solution, the record chromatogram drawn; With calculated by peak area, the results are shown in Table 2 by external standard method.
That quick capsule content determination experiment result of table 2. Buluoweima
Embodiment four: the mensuration of that quick granule content of Buluoweima
Get 10 bags of that quick particles of Buluoweima, remove unlap, accurate title is fixed; It is even to get contents mixed, porphyrize, and precision takes by weighing in right amount (being equivalent to brufen 40mg approximately) and puts in the 100ml measuring bottle, adds phosphate buffer to scale, and ultrasonic dissolution 30 minutes filters, and gets subsequent filtrate as need testing solution; Other gets reference substance brufen 40mg, pseudoephedrine hydrochloride 6mg and chlorphenamine maleate 0.4mg puts in the same 100ml measuring bottle, adds phosphate buffer ultrasonic dissolution 30 minutes, and is diluted to scale, shakes up, in contrast product solution; Under following selected chromatographic condition: VP-ODS chromatographic column (250mm * 4.6mm; 100A); With potassium dihydrogen phosphate (8.16g potassium dihydrogen phosphate and 2.8g lauryl sodium sulfate are put in the 1000ml measuring bottle, are dissolved in water, and are diluted to scale, shake up)-methyl alcohol-acetonitrile-triethylamine (40: 20: 40: 0.1) be moving phase (transferring pH to 4.5) with phosphoric acid; Detect wavelength 220nm; Number of theoretical plate must not calculate by the brufen peak and is lower than 3000.Accurate respectively need testing solution and each 20 μ l injection liquid chromatograph of reference substance solution, the record chromatogram drawn; With calculated by peak area, the results are shown in Table 3 by external standard method.
That quick particle content measuring experimental result of table 3. Buluoweima
Description of drawings
That quick assay high-efficient liquid phase chromatogram of accompanying drawing 1 Buluoweima
Brufen: 10.958min
Pseudoephedrine hydrochloride: 3.900min
Chlorphenamine maleate: 8.200min
That quick assay ultraviolet spectrum curve of accompanying drawing 2. Buluoweimas
1. brufen ultraviolet spectrum curve
2. pseudoephedrine hydrochloride ultraviolet spectrum curve
3. chlorphenamine maleate ultraviolet spectrum curve
Claims (5)
1. the detection method of content of an Ibuprofen, chlorphenamine maleate and Pseudoephedrine Hydrochloride compound preparation is characterized in that detection method may further comprise the steps:
(1) chromatographic condition: with octadecylsilane chemically bonded silica is filling agent; With potassium dihydrogen phosphate-methyl alcohol-acetonitrile-triethylamine is moving phase, and proportioning is 40: 20: 40: 0.1, wherein, potassium dihydrogen phosphate is put in the 1000ml measuring bottle by 8.16g potassium dihydrogen phosphate and 2.8g lauryl sodium sulfate, be dissolved in water, and be diluted to scale, shake up and form; Detect wavelength 215nm～225nm;
(2) preparation of need testing solution: it is an amount of to get Ibuprofen, chlorphenamine maleate and Pseudoephedrine Hydrochloride compound preparation, porphyrize, and precision takes by weighing the amount that is equivalent to brufen 40mg, put in the 100ml measuring bottle, added the phosphate buffer ultrasonic dissolution 30 minutes, and be diluted to scale, shake up, filter, get subsequent filtrate as need testing solution;
(3) preparation of reference substance solution: other gets reference substance brufen 40mg, pseudoephedrine hydrochloride 6mg and chlorphenamine maleate 0.4mg puts in the same 100ml measuring bottle, added the phosphate buffer ultrasonic dissolution 30 minutes, and be diluted to scale, shake up, in contrast product solution;
(4) content assaying method: accurate respectively absorption need testing solution and each 10 μ l～20 μ l of reference substance solution inject liquid chromatograph, the record chromatogram; Press external standard method respectively with brufen, pseudoephedrine hydrochloride and content for Chlorphenamine Maleate in the calculated by peak area sample.
2. the detection method of content of Ibuprofen, chlorphenamine maleate and Pseudoephedrine Hydrochloride compound preparation according to claim 1, it is characterized in that, wherein the moving phase of (1) chromatographic condition is made up of the mixed solution of potassium dihydrogen phosphate-methyl alcohol-acetonitrile-triethylamine, the ratio of each component is 40: 20: 40: 0.1, the pH value of moving phase is 4.0～5.0.
3. the detection method of content of Ibuprofen, chlorphenamine maleate and Pseudoephedrine Hydrochloride compound preparation according to claim 1, it is characterized in that, the wherein preparation of (3) reference substance solution: precision takes by weighing chlorphenamine maleate reference substance 20mg and puts in the 50ml measuring bottle, add the phosphate buffer dissolving and be diluted to scale, shake up, precision is measured 1ml and is put in the 100ml measuring bottle, other precision takes by weighing reference substance brufen 40mg and pseudoephedrine hydrochloride 6mg puts in the same 100ml measuring bottle, added the phosphate buffer ultrasonic dissolution 30 minutes, and be diluted to scale, shake up, in contrast product solution.
4. the detection method of content of Ibuprofen, chlorphenamine maleate and Pseudoephedrine Hydrochloride compound preparation according to claim 1 is characterized in that, wherein each Ibuprofen, chlorphenamine maleate and Pseudoephedrine Hydrochloride compound preparation unit contains brufen and should be 190～210mg; Hydrochloric pseudoephedrine should be 27.9～32.1mg; Contain chlorphenamine maleate and should be 1.86～2.14mg; Its content limit is 90%～110% of a standard content.
5. the detection method of content of Ibuprofen, chlorphenamine maleate and Pseudoephedrine Hydrochloride compound preparation according to claim 1, it is characterized in that the Ibuprofen, chlorphenamine maleate and Pseudoephedrine Hydrochloride compound preparation in this method can be tablet, capsule, granule, pill, powder or their controlled release and sustained release preparation.
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| CN101756981B (en) * | 2008-12-16 | 2013-04-10 | 北京科信必成医药科技发展有限公司 | Brufen loratadine pseudoephedrine release preparation and preparation method thereof |
| CN102890134B (en) * | 2012-05-22 | 2014-11-05 | 北京化工大学 | Method for measuring concentration of enantiomer of ibuprofen in chiral liquid-liquid extraction water phase by using high performance liquid chromatography (HPLC) |
| CN104133014B (en) * | 2014-07-16 | 2015-09-16 | 广州法尔麦兰药物技术有限公司 | A kind of method investigating Buluoweima sustained release preparation release |
| CN110297043A (en) * | 2018-03-21 | 2019-10-01 | 上海医药工业研究院 | A kind of analysis method of 2- methylamino -1- phenyl-1-propanol |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1473568A (en) * | 2003-04-14 | 2004-02-11 | 辽宁大生药业有限公司 | Medicinal composition containing Brufen for treating cold |
| WO2004038428A2 (en) * | 2002-10-25 | 2004-05-06 | Labopharm Inc. | Controlled-release compositions |
-
2006
- 2006-07-25 CN CNB2006100455572A patent/CN100387988C/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004038428A2 (en) * | 2002-10-25 | 2004-05-06 | Labopharm Inc. | Controlled-release compositions |
| CN1473568A (en) * | 2003-04-14 | 2004-02-11 | 辽宁大生药业有限公司 | Medicinal composition containing Brufen for treating cold |
Non-Patent Citations (12)
| Title |
|---|
| HPLC法同时测定布洛伪麻分散片中布洛芬盐酸伪麻黄碱的含量. 张文军等.天津医科大学学报，第9卷第1期. 2003 |
| HPLC法同时测定布洛伪麻分散片中布洛芬盐酸伪麻黄碱的含量. 张文军等.天津医科大学学报，第9卷第1期. 2003 * |
| HPLC法测定万辉化痰止咳露中磷酸可待因、盐酸麻黄碱和马来酸氯苯那敏的含量. 南楠等.药物分析杂志，第20卷第1期. 2000 |
| HPLC法测定万辉化痰止咳露中磷酸可待因、盐酸麻黄碱和马来酸氯苯那敏的含量. 南楠等.药物分析杂志，第20卷第1期. 2000 * |
| HPLC法测定洛芬伪麻片中的布洛芬和盐酸伪麻黄碱含量. 刘海凤等.中国现代应用药学，第14卷第4期. 1997 |
| HPLC法测定洛芬伪麻片中的布洛芬和盐酸伪麻黄碱含量. 刘海凤等.中国现代应用药学，第14卷第4期. 1997 * |
| 复方布洛芬片的高效液相色谱分析. 杜迎翔等.中国药科大学学报，第25卷第5期. 1994 |
| 复方布洛芬片的高效液相色谱分析. 杜迎翔等.中国药科大学学报，第25卷第5期. 1994 * |
| 布洛伪麻软胶囊含量测定方法的研究. 赵春顺等.中国药学杂志，第38卷第8期. 2003 |
| 布洛伪麻软胶囊含量测定方法的研究. 赵春顺等.中国药学杂志，第38卷第8期. 2003 * |
| 高效液相色谱法同时测定复方锌布颗粒中的布洛芬和马来酸氯苯那敏. 兰顺等.中国药事，第19卷第4期. 2005 |
| 高效液相色谱法同时测定复方锌布颗粒中的布洛芬和马来酸氯苯那敏. 兰顺等.中国药事，第19卷第4期. 2005 * |
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Address after: Xinluo Avenue high tech Zone of Ji'nan City, Shandong province 250101 No. 989 Shandong Academy of Pharmaceutical Sciences Room 401 Patentee after: Medicine Industry Inst., Shandong Prov. Address before: 250100 Shandong Pharmaceutical Industry Research Institute, 52 Da Bei Road, Shandong, Ji'nan Patentee before: Medicine Industry Inst., Shandong Prov. |
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Assignee: Guangdong Bozhou Pharmaceutical Co., Ltd. Assignor: Medicine Industry Inst., Shandong Prov. Contract record no.: 2010440000912 Denomination of invention: Content detecting method for Ibuprofen, chlorphenamine maleate and Pseudoephedrine Hydrochloride compound preparation Granted publication date: 20080514 License type: Exclusive License Open date: 20070103 Record date: 20100708 |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20080514 Termination date: 20150725 |
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| EXPY | Termination of patent right or utility model |