CN100376314C - An ultra permeable membrane of high polymer polystyrene resin and method for preparing same - Google Patents
An ultra permeable membrane of high polymer polystyrene resin and method for preparing same Download PDFInfo
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- CN100376314C CN100376314C CNB2006100346221A CN200610034622A CN100376314C CN 100376314 C CN100376314 C CN 100376314C CN B2006100346221 A CNB2006100346221 A CN B2006100346221A CN 200610034622 A CN200610034622 A CN 200610034622A CN 100376314 C CN100376314 C CN 100376314C
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- polystyrene resin
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- permeable membrane
- high polymer
- membrane
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Abstract
The present invention relates to the field of a biologic high molecular material, particularly to an ultra-permeable membrane of a high molecular polymer prepared from polystyrene resin and a preparation method thereof. The ultra-permeable membrane of a high molecular polymers prepared from polystyrene resin is composed of a basal membrane and the high molecular polymer prepared from the polystyrene resin, wherein a layer of resin high molecular polymer prepared from the polystyrene resin is uniformly coated on the basal membrane, and the ultra permeable membrane of a high molecular polymer prepared from polystyrene resin is formed after the resin high molecular polymer is dried, wherein the pore diameter of the ultra-permeable membrane is smaller than 30 nanometers. The ultra-permeable membrane of the present invention is used for the purification and the desalinization of DNA, protein and biological dyes, and the recovery ratio of a product is larger than 95%. The ultra-permeable membrane of the present invention can purify samples with the volume of smaller than one microlitre, which can not be achieved by other purifying methods.
Description
Technical field
The present invention relates to the bioabsorbable polymer material field, be specifically related to a kind of ultra permeable membrane of high polymer polystyrene resin and preparation method thereof.
Background technology
Ultra permeable membrane claims NF membrane again, it is the product between reverse osmosis membrane and milipore filter, with its normal-temperature operation, most processes do not undergo phase transition, low energy consumption, characteristics such as separative efficiency height obtain to use in a lot of fields, especially yield poorly the purification of the high product of tiring, separation and desalination in the field of biology.
Summary of the invention
The object of the present invention is to provide a kind of product recovery rate height, the ultra permeable membrane of high polymer polystyrene resin of the micro substance of can dialysing.
Another object of the present invention provides the preparation method of above-mentioned ultra permeable membrane of high polymer polystyrene resin.
Ultra permeable membrane of high polymer polystyrene resin of the present invention, form by basement membrane and high polymer polystyrene resin, described basement membrane is one or more the mixture in mixed cellulose ester, cellulose acetate class or the Polyurethane, and described basement membrane aperture is less than 0.8 micron; Described high polymer polystyrene resin is made up of following component and percetage by weight: polystyrene resin (PS) 1.0%～19%; The mixture 0.1%～5% of one or more in polyethylene (PE), polyvinyl chloride (PV), the polypropylene (PP); Chloroform or ethanol or its mixture 80%～98%.
The preparation method of above-mentioned ultra permeable membrane of high polymer polystyrene resin is: evenly be coated with one deck high polymer polystyrene resin on basement membrane, dry, form ultra permeable membrane of high polymer polystyrene resin, gained ultra permeable membrane aperture is less than 30 nanometers.
The present invention forms the ultra permeable membrane of the following different pore sizes of 30 nanometers by adjusting the concentration of polystyrene resin, and salt and hydrone are easy to by these apertures.DNA, protein and dye molecule are different by these aperture speed, reach to purify and the desalination purpose.
Compare with existing ultra permeable membrane, the present invention has following beneficial effect: ultra permeable membrane of the present invention is used for dialysis, and product recovery rate is more than 95%.DNA, protein, biological dye to the material that is low to moderate 1 microlitre can be purified and desalination, and this is that other purification process is difficult to realize.
The specific embodiment
Embodiment 1
Get polystyrene resin 8%, polyethylene 1%, chloroform 87%, ethanol 4% mixes the high polymer polystyrene resin that is made into percetage by weight 8%.
Get the mixed cellulose ester microporous membrane of 0.25 micron pore size, evenly be coated with the high polymer polystyrene resin of one deck 8% on this film, air dry under the room temperature forms ultra permeable membrane.The ultra permeable membrane of preparation is split and is divided into 4 parts, use 1/4 part at every turn, the every part of 1-50 μ l sample of can dialysing if amount is big, can be cut into 1/2 or do not cut off.Add 10-15ml ultra-pure water or distilled water in culture dish, dialysis membrane is floated on the water surface.The PCR product is directly placed on the dialysis membrane surface dialysis 15 minutes,, can prolong and carry out dialysis 20 minutes if strengthen dialysis-effect.The dialysis product purity height that obtains can be used for analyses such as the PCR product directly checks order, enzyme is cut, connection and uses.
Embodiment 2
Get polystyrene resin 6%, polyvinyl chloride and polypropylene miser 5%, chloroform 86%, ethanol 3% mixes the high polymer polystyrene resin that is made into percetage by weight 6%.
The high polymer polystyrene resin of even dip-coating 6% on the basement membrane described in the embodiment 1, air dry under the room temperature forms ultra permeable membrane.DNA is cut certain hour through restriction endonuclease A+ buffer A enzyme, and 65 ℃ were heated 10 minutes, and made enzyme A inactivation.Enzyme is cut product with described in the embodiment 1, places on the dialysis membrane surface to dialyse 15 minutes, removes buffer A.Draw dna solution with pipettor, add restriction endonuclease B+ buffer B enzyme and cut in dna solution, 65 ℃ were heated 10 minutes, and made enzyme B inactivation.Enzyme is cut product and is placed on the dialysis membrane surface dialysis 15 minutes, removes buffer B.Draw dna solution with pipettor, can obtain the DNA product cut of enzyme respectively through restriction endonuclease A and restriction endonuclease B.DNA rate of recovery height, dialysis product purity height can be used for follow-up bioanalysis process, does not introduce other organic solvent in the purge process and pollutes.
Claims (2)
1. ultra permeable membrane of high polymer polystyrene resin, it is characterized in that forming by basement membrane and high polymer polystyrene resin, described basement membrane is the miillpore filter of being made by mixed cellulose ester, cellulose acetate class or Polyurethane, and the aperture of basement membrane is less than 0.8 micron; Described high polymer polystyrene resin is made up of following component and percetage by weight: polystyrene resin 1.0%～19%; One or more mixture 0.1%～5% in polyethylene, polyvinyl chloride, the polypropylene; Chloroform or ethanol or its mixture 80%～98%; The ultra permeable membrane aperture is less than 30 nanometers.
2. the preparation method of the described ultra permeable membrane of high polymer polystyrene resin of claim 1 is characterized in that: evenly be coated with one deck high polymer polystyrene resin on basement membrane, drying, formation ultra permeable membrane of high polymer polystyrene resin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100346221A CN100376314C (en) | 2006-03-24 | 2006-03-24 | An ultra permeable membrane of high polymer polystyrene resin and method for preparing same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100346221A CN100376314C (en) | 2006-03-24 | 2006-03-24 | An ultra permeable membrane of high polymer polystyrene resin and method for preparing same |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1864831A CN1864831A (en) | 2006-11-22 |
| CN100376314C true CN100376314C (en) | 2008-03-26 |
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| Application Number | Title | Priority Date | Filing Date |
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| CNB2006100346221A Expired - Fee Related CN100376314C (en) | 2006-03-24 | 2006-03-24 | An ultra permeable membrane of high polymer polystyrene resin and method for preparing same |
Country Status (1)
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| CN (1) | CN100376314C (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5592103A (en) * | 1978-12-29 | 1980-07-12 | Toray Ind Inc | Selective, material-permeable complex membrane and preparing the same |
| JPS61141914A (en) * | 1984-12-13 | 1986-06-28 | Nok Corp | Gas separation membrane |
| JPH04243530A (en) * | 1991-01-25 | 1992-08-31 | Nok Corp | Antibacterial membrane |
| US5472607A (en) * | 1993-12-20 | 1995-12-05 | Zenon Environmental Inc. | Hollow fiber semipermeable membrane of tubular braid |
| CN1102610C (en) * | 1995-09-12 | 2003-03-05 | 三井化学株式会社 | Adhesive ethylenic polymer resin composition and laminate produced from the composition |
| US6635104B2 (en) * | 2000-11-13 | 2003-10-21 | Mcmaster University | Gas separation device |
| WO2006015495A1 (en) * | 2004-08-13 | 2006-02-16 | Mcmaster University | Composite material comprising a non-crosslinked gel polymer |
-
2006
- 2006-03-24 CN CNB2006100346221A patent/CN100376314C/en not_active Expired - Fee Related
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5592103A (en) * | 1978-12-29 | 1980-07-12 | Toray Ind Inc | Selective, material-permeable complex membrane and preparing the same |
| JPS61141914A (en) * | 1984-12-13 | 1986-06-28 | Nok Corp | Gas separation membrane |
| JPH04243530A (en) * | 1991-01-25 | 1992-08-31 | Nok Corp | Antibacterial membrane |
| US5472607A (en) * | 1993-12-20 | 1995-12-05 | Zenon Environmental Inc. | Hollow fiber semipermeable membrane of tubular braid |
| CN1102610C (en) * | 1995-09-12 | 2003-03-05 | 三井化学株式会社 | Adhesive ethylenic polymer resin composition and laminate produced from the composition |
| US6635104B2 (en) * | 2000-11-13 | 2003-10-21 | Mcmaster University | Gas separation device |
| WO2006015495A1 (en) * | 2004-08-13 | 2006-02-16 | Mcmaster University | Composite material comprising a non-crosslinked gel polymer |
Non-Patent Citations (1)
| Title |
|---|
| 高分子复合物的研究与应用进展. 罗坤，尹静波，陈红丹.高分子通报，第1期. 2006 * |
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| CN1864831A (en) | 2006-11-22 |
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Granted publication date: 20080326 Termination date: 20110324 |