CN100354410C - High-efficient bauxite microorganism ore dressing bacterial and screening process thereof - Google Patents
High-efficient bauxite microorganism ore dressing bacterial and screening process thereof Download PDFInfo
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- 229910001570 bauxite Inorganic materials 0.000 title claims abstract description 45
- 244000005700 microbiome Species 0.000 title claims abstract description 22
- 230000001580 bacterial effect Effects 0.000 title claims description 56
- 238000000034 method Methods 0.000 title abstract description 19
- 238000012216 screening Methods 0.000 title abstract description 17
- 230000012010 growth Effects 0.000 claims abstract description 19
- 241000228245 Aspergillus niger Species 0.000 claims abstract description 10
- 241000894006 Bacteria Species 0.000 claims description 25
- 239000007788 liquid Substances 0.000 claims description 20
- 229930006000 Sucrose Natural products 0.000 claims description 10
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 10
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 239000011324 bead Substances 0.000 claims description 5
- 230000004888 barrier function Effects 0.000 claims description 4
- 230000015572 biosynthetic process Effects 0.000 claims description 4
- 210000000557 podocyte Anatomy 0.000 claims description 4
- 229920001817 Agar Polymers 0.000 claims description 3
- 244000061456 Solanum tuberosum Species 0.000 claims description 3
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 3
- 230000009603 aerobic growth Effects 0.000 claims description 3
- 239000008272 agar Substances 0.000 claims description 3
- 238000004321 preservation Methods 0.000 claims description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 abstract description 22
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- 230000000694 effects Effects 0.000 abstract description 12
- 229910052742 iron Inorganic materials 0.000 abstract description 9
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- 230000002349 favourable effect Effects 0.000 abstract 1
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- 239000002609 medium Substances 0.000 description 10
- KMWBBMXGHHLDKL-UHFFFAOYSA-N [AlH3].[Si] Chemical compound [AlH3].[Si] KMWBBMXGHHLDKL-UHFFFAOYSA-N 0.000 description 9
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- 230000004151 fermentation Effects 0.000 description 6
- 238000005065 mining Methods 0.000 description 6
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- 238000013019 agitation Methods 0.000 description 3
- 229940037003 alum Drugs 0.000 description 3
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
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- 239000005995 Aluminium silicate Substances 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- BPQQTUXANYXVAA-UHFFFAOYSA-N Orthosilicate Chemical compound [O-][Si]([O-])([O-])[O-] BPQQTUXANYXVAA-UHFFFAOYSA-N 0.000 description 1
- 240000000111 Saccharum officinarum Species 0.000 description 1
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- 229910004298 SiO 2 Inorganic materials 0.000 description 1
- 235000012211 aluminium silicate Nutrition 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
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- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
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- 229910052751 metal Inorganic materials 0.000 description 1
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Abstract
The present invention relates to bauxite ore microorganism ore dressing strain aspergillus niger and a screening method thereof, which is characterized in that a high-efficient bauxite ore microorganism ore dressing strain is aspergillus niger preserved in the common microorganism center of the China Committee for Culture Collection of Microorganisms, and the accession number of the aspergillus niger is CGMCC NO. 1539. The screening methods for strains comprise: (1) sample collection, (2) strain screening, (3) strain growth conditions and (4) performance detection. A microorganism strain screened by the screening method exists in soil and ore. The microorganism strain has favorable bauxite ore iron removing effect and certain desiliconization effect by measurement.
Description
Technical field
The present invention relates to a kind of bauxite microorganism ore dressing bacterial aspergillus niger (Aspergillus niger) and screening method thereof.
Background technology
Al in China's bauxite
2O
3Content higher, but since ore in contain higher SiO simultaneously
2, so the alumina silica ratio of ore is totally lower.Statistics are arranged, account in 255 mining areas of 7 provinces and regions such as Shanxi, Henan, Guangxi, Guizhou, Shandong and Sichuan, Yunnan of China's bauxite total reserves 96%, the bauxite average grade is: Al
2O
361.99%, SiO
210.4%, Fe
2O
37.73%, the average alumina silica ratio of ore only is 5.96.And in 307 bauxite mining areas, the whole nation, according to statistics, A/S has only 7 greater than 10 mining area, and reserves only account for 6.97%, and only having, the A/S of half reserves bauxite is between the 4-6.For reducing the silicon content in the bauxite, mainly the method by ore dressing realizes that aluminium silicon separates i.e. physical concentration aluminium silicon separation, the separation of chemical mineral processing aluminium silicon etc.Wherein in the physical concentration aluminium silicon partition method research maximum be flotation process, it comprises again, and direct flotation aluminium silicon separates, reverse flotation aluminium silicon separates.What research was more in the chemical mineral processing aluminium silicon partition method is roasting-alkaline leaching.Except physical concentration method and chemical mineral processing method can realize that bauxite aluminium-silicon separates, adopt biological beneficiation method can realize that also the aluminium silicon of bauxite separates, and improves the alumina silica ratio of ore.
The microorganism that has can produce metabolism products such as citric acid and oxalic acid during the fermentation, and these products can leach the valuable metal in the oxide mineral.Have report to show, microorganisms such as silicate bacteria can go the silicon in the bauxite is molten, can improve the quality of bauxite.There are some tunnings that produce sour fungi not only can leach Al in the kaolin in addition
2O
3, can also effectively leach the impurity iron in the bauxite, the bauxite that removes impurity iron can be produced the fine refractory materials.Therefore at the bauxite resource characteristics of China, carry out the efficient microbial strain that the research of bauxite biological metallurgy, particularly screening have desiliconization from bauxite, remove ferrum property, have important in theory and using value.
Summary of the invention
The objective of the invention is to screen bauxite is had the microbial strains of desiliconization, de-ferrous effect and provides such bacterial screening isolating method.
The objective of the invention is to be achieved through the following technical solutions.
A kind of bauxite microorganism ore dressing bacterial, it is characterized in that this bacterium is the black-koji mould (Aspergillus niger) with strong product organic acid ability, in the common micro-organisms center preservation of China Committee for Culture Collection of Microorganisms of specified depositary institution of Patent Office of the People's Republic of China, deposit number is: CGMCC NO.1539.
A kind of bauxite microorganism ore dressing bacterial of the present invention is characterized in that form of this bacterium and culture condition are:
1) this bacterial classification is grown on the Cha Shi substratum, and the bacterium colony size 5d-10d of formation reaches 30mm-50mm, and the positive color of bacterium colony is white-yellowish-brown-black, deepens gradually, and middle body forms a large amount of black soruss.The bacterium colony circle, neat in edge, bacterium colony back side color is colourless, the later stage becomes light yellow; This bacterial classification hyphae colorless, the fine hair shape, closely, later stage surface cohesion atrament; Conidiophore is born from the heavy wall podocyte on the mycelia, and is upright, and most no barrier films are thick; Shake a bottle shaking culture 2d-7d in the liquid medium within, form a large amount of white hypha body beads, the pH value of substratum drops to 1.5-3.5.
2) solid medium: potato 200g, sucrose 10g, agar 15g-20g, water 1000mL, the purpurum bromocresolis ethanolic soln 1mL-2mL of adding 1.6%, pH nature, 121 ℃ of sterilization 20min;
Liquid nutrient medium: NaNO
32g, K
2HPO
41g, KCl 0.5g, MgSO
40.5g, FeSO
40.01g, sucrose 15g, water 1000mL, pH nature, 121 ℃ of sterilization 20min;
3) growth temperature is 20 ℃-35 ℃, and optimum growth temperature is 25 ℃-30 ℃, and pH 2.5-7.5 can grow, and optimal pH is 5.5-6.5, aerobic growth.
The screening method of high-efficient bauxite microorganism ore dressing bacterial of the present invention is characterized in that: from alum clay mining area acquisition tables layer soil and bauxitic ore sample, about 1m
2The zone in gather the soil sample or the sample ore of 5 points, each point is gathered about 200g sample, is respectively charged into after soil sample or sample ore mix in the paper bag of sampling; Be equipped with and add 10g soil sample or sample ore in the beaker of 100mL aqua sterilisa, fully stir 10min, desorptions such as the microbial spore that is adsorbed on the mineral grain, mycelium are entered in the aqueous solution, be made into suspension; Adopt dull and stereotyped isolation by dilution method to separate, under aseptic technique, get the 1mL suspension in the culture dish of high-temperature sterilization, pour 10mL then into and be cooled to substratum about 40 ℃, mix, fully the cooling back the solid separating plate.Flat board is inverted in the biochemical incubator, cultivates 2d-7d under 25 ℃ of-30 ℃ of conditions, the growth site substratum is become the xanchromatic bacterium colony by purple choose, carry out purifying and cultivate, screen.
The microbial strains of the present invention's screening is present in soil and the ore, and after measured, this bacterial classification has good bauxite de-ferrous effect and certain desiliconization effect.
Description of drawings
Fig. 1: bacterial screening technological line figure;
Fig. 2: growth curve in the bacterial classification liquid medium within;
Fig. 3: the substrate sucrose concentration produces the influence of acid to bacterial classification;
Fig. 4: culture temperature is produced the influence of acid to bacterial classification;
Fig. 5: different shaking speed are produced the influence of acid to bacterial classification.
Embodiment
The bauxite microorganism ore dressing bacterial strain screening method is as follows:
1, sample collecting: from alum clay mining area acquisition tables layer soil and bauxitic ore sample, a 1m
2The zone in gather the soil sample or the sample ore of 5 points, each point is gathered about 200g sample, is respectively charged in the paper bag of sampling after soil sample or sample ore mix, and separates the microbial strains with ore concentration of bauxite effect from the sample of being gathered in the laboratory.
2, bacterial screening: each is equipped with and adds isolating soil sample of 10g desire or sample ore in the beaker of 100mL aqua sterilisa, fully stirs 10min, and desorptions such as the microbial spore that is adsorbed on the mineral grain, mycelium are entered in the aqueous solution, is made into suspension, for separating use.The separatory selective medium component of solid is: potato 200g, sucrose 10g, agar 15g-20g, water 1000mL, the purpurum bromocresolis ethanolic soln 1-2mL of adding 1.6%, pH nature, 121 ℃ of sterilization 20min.The liquid nutrient medium of growth: NaNO
32g, K
2HPO
41g, KCl 0.5g, MgSO
40.5g, FeSO
40.01g, sucrose 15g, water 1000mL, pH nature, 121 ℃ of sterilization 20min.Adopt dull and stereotyped isolation by dilution method to separate, under aseptic technique, get the 1mL suspension in the culture dish of high-temperature sterilization, pour 10mL then into and be cooled to above-mentioned selective medium about 40 ℃, mix, fully the cooling back solid plate.Flat board is inverted in constant temperature culture 2d-7d in 25 ℃-30 ℃ the biochemical incubator, the growth site substratum become the xanchromatic bacterium colony by purple choose, move on the solid plate or in the liquid nutrient medium, carry out purifying and cultivate.Product organic acid determination of activity by bacterial strain and the leaching effect of impurity iron mineral and silicon mine in the bauxite measured, it is fast therefrom to select growth, and it is active strong to produce organic acid, and bauxite is soaked iron, bacterial strain that desiliconization effect is good.
Selected high-efficient bauxite microorganism ore dressing bacterial is to have the active black-koji mould of strong product organic acid (Aspergillusniger).This bacterial classification is grown on czapek's solution, and the bacterium colony size 5d-10d of formation reaches 30mm-50mm, and the positive color of bacterium colony is white-yellowish-brown-black, deepens gradually, and middle body forms a large amount of black soruss.The bacterium colony circle, neat in edge, bacterium colony back side color is colourless, the later stage becomes light yellow.Bacterial classification hyphae colorless look, the fine hair shape, closely, later stage surface cohesion atrament; Conidiophore is born from the heavy wall podocyte on the mycelia, and is very upright, and most no barrier films are thick.Shake a bottle shaking culture 2d-7d in the bacterial classification liquid medium within, form a large amount of white hypha body beads, the pH value of substratum drops to 1.5-3.5.
3, the growth conditions of bacterial classification: adopt simple interest method and gravimetry research microbial growth situation, bacterial strain produces the difference of sour behavior under the research different sucrose, the difference of strain growth behavior is studied the influence of different shaking speed to strain growth under the research differing temps.The growth conditions of this bacterial classification is: 20 ℃-35 ℃ of growth temperatures, and 25 ℃-30 ℃ of optimum growth temperatures, pH 2.5-7.5, optimal pH are 5.5-6.5, aerobic growth.
4, the Performance Detection of bacterial classification
1) bacterial classification deironing: under 30 ℃, 200r/min condition, cultivate 7d, when the pH of fermented liquid reduces to 1.5-2.5, cross leakage, collect fermented liquid, use 1M H with double-deck filter paper
2SO
4Solution is transferred the pH to 0.5-1.0 of fermented liquid, as soaking the ore deposit agent.The 100mL that packs in capacity is the Erlenmeyer flask of 250mL soaks the ore deposit agent, adds the bauxite of the impure iron of 10g, 90 ℃ of following agitation leach 6h.Leak paper with bilayer and cross leakage ore pulp, collect and soak slag, 80 ℃ dry down, leached mud and ore leachate detected the removal effect of impurity iron in the research bauxite.
2) bacterial classification desiliconization: the 100mL fermention medium of in capacity is the Erlenmeyer flask of 250mL, packing into, inoculate a small amount of microbial spore, under 30 ℃, 200r/min condition, cultivate 7d, when the pH of fermented liquid reduces to 1.5h-2.5h, add the 10g particle diameter less than 100 purpose low-grade bauxite stones, continue to cultivate 5d-7d.Cross leakage ore pulp with the accurate testing sieve of specification 45 targets, ore pulp natural subsidence 5min collects settled leached mud.Leached mud carries out chemical ingredients and detects after 80 ℃ of following dryings, research bauxite microorganism desiliconization effect.
Below further specify the screening method of high-efficient bauxite microorganism ore dressing bacterial by example, concrete steps are as follows:
1, sample collecting
From Luoyang, Henan Jia Gou alum clay mining area acquisition tables layer soil and bauxitic ore, a 1m
2The zone in gather the soil sample or the sample ore of 5 points, each point is gathered about 200g sample, is respectively charged in the paper bag of sampling after soil sample or sample ore mix, and separates the microorganism strains with ore concentration of bauxite effect from the sample of being gathered in the laboratory.
2, bacterial screening
Adopt dull and stereotyped isolation by dilution method to carry out the screening of leaching microbacteria bacterial classification, separating plate is cultivated 2d-7d down at 25 ℃-30 ℃, successively there is the minority bacterium colony to grow, can produce the organic acid bacterial classification can make the periphery of bacterial colonies substratum become the xanchromatic bacterium colony by purple to choose, wherein the yellow circle of strain black-koji mould generation is big, acid producing ability is strong especially, in the invention this Aspergillus niger strain is studied as the leaching microbacteria bacterial classification.
3, the biological characteristics of bacterial classification
Bacterial classification is to have the active black-koji mould of strong product organic acid (Aspergillus niger).This bacterial classification is grown on czapek's solution, and the bacterium colony size 5d-10d of formation reaches 30mm-50mm, and the positive color of bacterium colony is white-yellowish-brown-black, deepens gradually, and middle body forms a large amount of black soruss.The bacterium colony circle, neat in edge, bacterium colony back side color is colourless, the later stage becomes light yellow.The bacterial classification hyphae colorless, the fine hair shape, closely, later stage surface cohesion atrament; Conidiophore is born from the heavy wall podocyte on the mycelia, and is very upright, and most no barrier films are thick.At 30 ℃, the condition liquid culture 7d of 200r/min, measure mycelial dry weight, the growth curve that obtains is seen Fig. 2.Find to form in the nutrient solution mycelium bead of many whites, along with the prolongation of incubation time, it is big that the volume of mycelium bead constantly becomes, and quantity is on the increase, and illustrates that the nutritive substance that this bacterial classification can make full use of in the substratum grows.
4, strain fermentation condition
1) the substrate sucrose concentration produces the influence of acid amount to bacterial classification
Bacterial classification was respectively in the nutrient solution of 10g/L, 15g/L, 20g/L, 25g/L, 30g/L shaking culture 7 days at sucrose concentration, pH value difference differnce Fig. 3 of each fermented liquid.Cultivate after 7 days, the fermented liquid pH value that the half congealed concentration of substrate sugarcane is respectively 10g/L, 15g/L, 20g/L, 25g/L, 30g/L drops to 2.51,2.42,2.24,2.13,1.98 successively.Explanation is under study condition of the present invention, and when concentration of substrate was big, the organic acid that strain fermentation produces was also many.
2) culture temperature is produced the influence of acid and increment to bacterial classification
Bacterial classification is difference oscillation and fermentation cultivation 5d under 25 ℃, 30 ℃, 35 ℃ three kinds of temperature, and the fermented liquid pH value under the differing temps is seen Fig. 4.As can be seen, the pH value was minimum when bacterial classification was 30 ℃ in culture temperature, produced acid at most, and mycelial biomass is also maximum simultaneously, illustrate that bacterial strain is adapted at growing and carrying out organic acid under 30 ℃ of the temperature fermenting most.
3) different shaking speed are produced the influence of acid to bacterial classification
Bacterial classification is under 100r/min, 150r/min, 200r/min, 250r/min, five kinds of rotating speeds of 300r/min, and fermentation culture 7d measures the pH value of different rotating speeds bottom fermentation liquid, the results are shown in Figure 5.Explanation is along with the raising of rotating speed, and the product of bacterial classification acid amount increases, and biomass also increases.Its reason is within the specific limits, and along with rotating speed improves, the quantity of effective dissolved oxygen increases in the nutrient solution, and the growth and breeding of urging in aerobic microbiological is arranged.
5, bacterial classification Performance Detection
Example 1 soaks the experiment 1 except that impurity iron mineral in the bauxitic ore
With the 1# bauxite of the impure iron of 100mL acidifying ore leachate leaching 10g, 80 ℃ of following agitation leach 6h.Fe in the bauxitic ore
2O
3Content drops to 0.69% after the leaching by 4.56% before leaching.And carry out same experiment with the sulphuric acid soln of same pH value, Fe in the ore
2O
3Content only removes preceding 4.56% and drops to 3.09% from soaking.
Example 2 soaks the experiment 2 except that impurity iron mineral in the bauxitic ore
With the 2# bauxite of the impure iron of 100mL acidifying ore leachate leaching 10g, 90 ℃ of following agitation leach 6h.Fe in the bauxitic ore
2O
3Content drops to 0.48% after the leaching by 6.36% before leaching.And carry out same experiment with the sulphuric acid soln of same pH value, Fe in the ore
2O
3Content only removes preceding 6.36% and drops to 4.57% from soaking.
The experiment 1 that example 3 leaches the impurity silicon mine in the bauxitic ore
The 100mL liquid nutrient medium of in capacity is the Erlenmeyer flask of 250mL, packing into, bacterial classification was cultivated 7 days under 30 ℃, 200r/min condition, when the pH of fermented liquid reduces to 1.5-2.5, add the low-grade bauxite stone of 10g particle diameter less than 174 μ m, continue to cultivate 5d-7d days, be that 45 purpose normal test sieves filter with specification then, collect settled bauxite and soak slag, detect after 80 ℃ of dryings.Find the SiO of leached mud
2Content 13.3% drops to 8.0% before leach, and A/S brings up to 8.33 from 4.43.
The experiment 2 that example 4 leaches the impurity silicon mine in the bauxitic ore
The 100mL liquid nutrient medium of in capacity is the Erlenmeyer flask of 250mL, packing into, bacterial classification was cultivated 7 days under 30 ℃, 200r/min condition, when the pH of fermented liquid reduces to 1.8, add the low-grade bauxite stone of 10g particle diameter less than 174 μ m, continue to cultivate 6 days, be that 45 purpose normal test sieves filter with specification then, collect settled bauxite and soak slag, detect after 80 ℃ of dryings.Find the SiO of leached mud
2Content 11.6% drops to 6.7% before leach, and A/S brings up to 10.45 from 7.33.
Claims (2)
1, a kind of bauxite microorganism ore dressing bacterial, it is characterized in that this bacterium is the black-koji mould (Aspergillus niger) with strong product organic acid ability, in the common micro-organisms center preservation of China Committee for Culture Collection of Microorganisms of specified depositary institution of Patent Office of the People's Republic of China, deposit number is: CGMCC NO.1539.
2, a kind of bauxite microorganism ore dressing bacterial according to claim 1 is characterized in that form of this bacterium and culture condition are:
1) this bacterial classification is grown on the Cha Shi substratum, and the bacterium colony size 5d-10d of formation reaches 30mm-50mm, and the positive color of bacterium colony is white-yellowish-brown-black, deepens gradually, and middle body forms a large amount of black soruss; The bacterium colony circle, neat in edge, bacterium colony back side color is colourless, the later stage becomes light yellow; This bacterial classification hyphae colorless, the fine hair shape, closely, later stage surface cohesion atrament; Conidiophore is born from the heavy wall podocyte on the mycelia, and is upright, and most no barrier films are thick; Shake a bottle shaking culture 2d-7d in the liquid medium within, form a large amount of white hypha body beads, the pH value of substratum drops to 1.5-3.5;
2) Cha Shi solid medium: potato 200g, sucrose 10g, agar 15g-20g, water 1000mL, the purpurum bromocresolis ethanolic soln 1mL-2mL of adding 1.6%, pH nature, 121 ℃ of sterilization 20min;
Liquid nutrient medium: NaNO
32g, K
2HPO
41g, KCl 0.5g, MgSO
40.5g, FeSO
40.01g, sucrose 15g, water 1000mL, pH nature, 121 ℃ of sterilization 20min;
3) growth temperature is 20 ℃-35 ℃, and optimum growth temperature is 25 ℃-30 ℃, and pH2.5-7.5 can grow, and optimal pH is 5.5-6.5, aerobic growth.
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| CNB2005101240094A CN100354410C (en) | 2005-11-28 | 2005-11-28 | High-efficient bauxite microorganism ore dressing bacterial and screening process thereof |
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|---|---|---|---|
| CNB2005101240094A CN100354410C (en) | 2005-11-28 | 2005-11-28 | High-efficient bauxite microorganism ore dressing bacterial and screening process thereof |
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| Publication Number | Publication Date |
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| CN102861674B (en) * | 2012-09-29 | 2014-11-19 | 北京科技大学 | Floatation processing method of pyrolusite |
| CN108530092A (en) * | 2018-06-06 | 2018-09-14 | 湖南化工职业技术学院（湖南工业高级技工学校） | A kind of method that bauxite gangue prepares low iron refractory brick base-material coproduction nano-sized iron oxide |
| CN109593952A (en) * | 2018-12-20 | 2019-04-09 | 太原理工大学 | A kind of method of pyroxene or hornblend in removing magnetic separation of iron ore concentrate |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3218240A (en) * | 1962-02-02 | 1965-11-16 | Nat Res Dev | Device and method for testing mineral deficiencies in soil |
| SU1604843A1 (en) * | 1988-12-30 | 1990-11-07 | Институт микробиологии АН СССР | Method of processing synnyrite |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3218240A (en) * | 1962-02-02 | 1965-11-16 | Nat Res Dev | Device and method for testing mineral deficiencies in soil |
| SU1604843A1 (en) * | 1988-12-30 | 1990-11-07 | Институт микробиологии АН СССР | Method of processing synnyrite |
Non-Patent Citations (2)
| Title |
|---|
| Fungal degradation of calcium-,lead- and silicon-bearing minerals. Adeyemi AO,Gadd GM.BIOMETALS,Vol.18 No.3. 2005 * |
| 两株溶磷黑曲霉的生长特性及对不同难溶性磷酸盐的溶解作用 刘晓芳，黄晓东，刘晔.山东农业大学学报(自然科学版)，第36卷第2期 2005 * |
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