CN100352450C - Albumen removed extraction of calf blood for injection and its preparing process - Google Patents
Albumen removed extraction of calf blood for injection and its preparing process Download PDFInfo
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- CN100352450C CN100352450C CNB2004100135096A CN200410013509A CN100352450C CN 100352450 C CN100352450 C CN 100352450C CN B2004100135096 A CNB2004100135096 A CN B2004100135096A CN 200410013509 A CN200410013509 A CN 200410013509A CN 100352450 C CN100352450 C CN 100352450C
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Abstract
The present invention relates to deproteinized calf blood extract for injection and a preparing method of the deproteinized calf blood extract. When the deproteinized calf blood extract is in solution state, organic substances such as polypeptide, amino acid, ribonucleic acid, etc. are polymerized, oxidized and condensed to generate high molecular substances, and thus, the allergic reaction of medicines is increased. The deproteinized calf blood extract for injection comprises deproteinized calf blood extract, wherein the deproteinized calf blood extract is extracted from fresh calf blood or blood serum, solid substances of the deproteinized calf blood extract contain 0.10 to 0.90 part of total nitrogen and 0.01 to 0.40 part of free amino acid by weight and have the pH value of 3.0 to 9.0 and the molecular weight of 5, 000 to 10, 000, and ninhydrin reaction is positive. The deproteinized calf blood extract is used for treating cerebral cell metabolism dysbolism diseases such as cerebral ischemia, cerebral dementia, cerebral trauma, insufficiency of cerebral functions, etc., and can also be used for treating circulatory disturbance peripheral arteries and veins, and artery vascular diseases caused by the circulatory disturbance.
Description
Technical field: the present invention relates to a kind of deproteinised calf blood extract for injection and its prepration technology.
Background technology: calf blood protein-removed extraction is by oligosaccharide, nucleic acid derivative, aminoacid, the material of the biologically active that Organic substance such as low molecular weight polypeptide and inorganic matter are formed, calf blood protein-removed extraction under solution state because polypeptide, aminoacid, polymerization can take place in Organic substances such as nucleic acid, oxidation, reaction such as condensation produces polymer substance, cause that the solution clarity descends, even in clear and bright solution often also because the existence of polymer substance, and the anaphylaxis of medicine is increased, biological activity descends, thereby patient is damaged, in addition, calf blood protein-removed extraction solution needs cryopreservation, south hospital pharmacy temperature in summer can reach more than 35 ℃ in China, in some, infirmary, the pharmacy does not possess freezer, therefore have a strong impact on drug quality, medicine can produce serious Organic substance and separate out, the anaphylaxis of medicine is increased the weight of, thereby had a strong impact on the use of calf blood protein-removed extraction solution, be unfavorable for this medicine long preservation and promote the use of.
Summary of the invention: it is good to the purpose of this invention is to provide a kind of clinical efficacy, and preparation technology is simple, the deproteinised calf blood extract for injection and its prepration technology of safe and convenient to use and easy storage.
The object of the present invention is achieved like this:
The injection calf blood protein-removed extraction, its composition comprises: calf blood protein-removed extraction, described calf blood protein-removed extraction is to extract from fresh calf blood or serum, described calf blood protein-removed extraction solid content contains total nitrogen, free amino acid, its ratio of weight and number is a total nitrogen 0.10～0.90, free amino acid 0.01～0.40, and pH value should be 3.0～9.0, molecular weight is 5000～10000, and the reaction of indenes three bronze medals is positive.
The preparation technology of above-mentioned injection calf blood protein-removed extraction, calf blood protein-removed extraction solution is put into freeze drying box, goods are refrigerated to-45 ℃～20 ℃, cold-trap drops to-45 ℃～20 ℃, evacuation makes drying baker vacuum reach 1～10Pa, during primary drying, the conduction oil temperature is set in-5 ℃～-20 ℃, goods are sublimation drying at the uniform velocity, during redrying, the conduction oil temperature is set in 30～35 ℃, product dried when products temperature reaches 35 ℃, continues dry until up to specification, it is off-white color or faint yellow block or amorphous powder that the goods bone dry obtains outward appearance, make aseptic freeze-dried product, and press 100mg, 200mg, 400mg, 600mg and 800mg specification packing can be used for intravenous injection, intra-arterial injection, intramuscular injection, also can add 200-300ml 5% glucose or 0.9% sodium chloride injection iv drip, the about 2ml/min of drip velocity.
The preparation technology of above-mentioned calf blood protein-removed extraction solution, get above-mentioned calf blood or calf serum, acid adjustment pH value to 3.0～5.5, transfer alkaline pH value to 8.0～10.5 again, after the accent neutrality, be heated to 30～80 ℃, go precipitation, use the ultrafilter membrane ultrafiltration then, the molecular weight that dams is 5000～10000 dalton, collects filtrate and is calf blood or calf serum protein-removing extract solution.
The invention has the advantages that:
1. injection calf blood protein-removed extraction of the present invention contains inorganic elementss such as multiple small-molecule active substance and calcium, phosphorus, ferrum, its pharmacological action is by promoting picked-up and the utilization of cell to glucose, promote picked-up and the utilization of cell to oxygen, thereby ATP is generated to be increased, the cell energy amount is improved, when (low blood oxygen, substrate deficiency) and energy requirement increase under function defective, the downtrod situation of metabolism, can promote the cellular energy metabolism, improve cell function.
2. the present invention adopts modern biotechnology to make calf blood protein-removed extraction solution, and adopt the dry technology of low-temperature freeze drying to make aseptic freeze-dried injectable powder, it not only has advantages such as the calf blood protein-removed extraction solution effects is direct, quick, and kept the biological activity of component to greatest extent, be easy to transportation and preservation, guaranteed the curative effect of this product.
3. the present invention compares with calf blood protein-removed extraction solution, and this product is good stability in the process of storage, and polymerization does not take place for oligosaccharide, nucleic acid derivative, aminoacid and low molecular weight polypeptide, has reduced the anaphylaxis incidence rate, has guaranteed patient's drug safety.
4. this product is a lyophilized injectable powder, can preserve under ℃ condition of temperature 20～-40, has saved operation cost, has increased the economic benefit of this medicine.
5. this product can promote picked-up and the utilization of cell to glucose and oxygen, under low blood oxygen and energy such as need increase at situation, this product can promote energy metabolism, increase the blood flow of internal organs such as the heart, brain, liver, microcirculation improvement, be applicable to the not congruent brain cell dysbolismus of cerebral ischemia, brain dementia, cerebral trauma and brain function disease, clinical tip tremulous pulse, venous circulation obstacle and the artery vessel disease that causes, the leg ulcer of also can be used for; Skin grafting; Skin burn, scald, erosion; Healing of wound (wound, decubital ulcer); Radio-induced skin, mucosa injury.
Description of drawings: accompanying drawing 1 is technology path of the present invention and process chart.
Below in conjunction with description of drawings the specific embodiment of the present invention:
Embodiment 1:
Deproteinised calf blood extract for injection and its prepration technology, its composition comprises: calf blood protein-removed extraction, described calf blood protein-removed extraction is to extract from fresh calf blood or serum, described calf blood protein-removed extraction solid content contains total nitrogen, free amino acid, its ratio of weight and number is a total nitrogen 0.10, free amino acid 0.01, pH value should be 3.0, molecular weight is 5000, the reaction of indenes three bronze medals is positive, its preparation technology is: calf blood protein-removed extraction solution is put into freeze drying box, goods are refrigerated to-45 ℃, cold-trap drops to-45 ℃, evacuation makes drying baker vacuum reach 1Pa, during primary drying, the conduction oil temperature is set in-5 ℃, goods are sublimation drying at the uniform velocity, during redrying, the conduction oil temperature is set in 30 ℃, product dried, when products temperature reaches 35 ℃, continue dry until up to specification, it is off-white color or faint yellow block or amorphous powder that the goods bone dry obtains outward appearance, make aseptic freeze-dried product, and press 100mg, 200mg, 400mg, 600mg and 800mg specification packing can be used for intravenous injection, intra-arterial injection, intramuscular injection, also can add 200ml 5% glucose or 0.9% sodium chloride injection iv drip, the about 2ml/min of drip velocity is to brain ischemic lesions (apoplexy and cerebral trauma), the intravenous drip of a 0.8g total solid, once-a-day, two weeks were a course of treatment; Brain function is not reached brain dementia entirely, the intravenous injection of a 1.2g total solid, 1 time on the one, two weeks were a course of treatment; To artery vessel disease, the intravenous drip of a 0.8g total solid, 1 time on the one or 0.8g total solid tremulous pulse or intravenous injection, weekly for several times, course of treatment all around; To shank or other chronic ulcers, burn, each quiet notes of 0.4g total solid (or the intramuscular injection of 0.2g total solid) 1 time on the one or weekly for several times, can add by healing state and to use this product topical therapeutic; To the prevention and the treatment of radioactive skin, mucosa injury, at radiation period, the average one day quiet notes of 0.2g total solid; To radiocystitis, 0.4g total solid associating antibiotic therapy on the one, per urethra administration.
Embodiment 2:
Deproteinised calf blood extract for injection and its prepration technology, its composition comprises: calf blood protein-removed extraction, described calf blood protein-removed extraction is to extract from fresh calf blood or serum, described calf blood protein-removed extraction solid content contains total nitrogen, free amino acid, its ratio of weight and number is a total nitrogen 0.90, free amino acid 0.40, pH value should be 9.0, molecular weight is 10000, the reaction of indenes three bronze medals is positive, its preparation technology is: calf blood protein-removed extraction solution is put into freeze drying box, goods are refrigerated to-20 ℃, cold-trap drops to-20 ℃, evacuation makes drying baker vacuum reach 10Pa, during primary drying, the conduction oil temperature is set in-20 ℃, goods are sublimation drying at the uniform velocity, during redrying, the conduction oil temperature is set in 35 ℃, product dried, when products temperature reaches 35 ℃, continue dry until up to specification, it is off-white color or faint yellow block or amorphous powder that the goods bone dry obtains outward appearance, make aseptic freeze-dried product, and press 100mg, 200mg, 400mg, 600mg and 800mg specification packing can be used for intravenous injection, intra-arterial injection, intramuscular injection, also can add 300ml 5% glucose or 0.9% sodium chloride injection iv drip, the about 2ml/min of drip velocity is to brain ischemic lesions (apoplexy and cerebral trauma), the intravenous drip of a 1.2g total solid, once-a-day, two weeks were a course of treatment; Brain function is not reached brain dementia entirely, the intravenous injection of a 1.2g total solid, 1 time on the one, two weeks were a course of treatment; To artery vessel disease, once-intravenous drip of 2.0g total solid, 1 time on the one or 2g total solid tremulous pulse or intravenous injection, weekly for several times, course of treatment all around; To shank or other chronic ulcers, burn, each quiet notes of 0.4g total solid (or the intramuscular injection of 0.2g total solid) 1 time on the one or weekly for several times, can add by healing state and to use this product topical therapeutic; To the prevention and the treatment of radioactive skin, mucosa injury, at radiation period, the average one day quiet notes of 0.2g total solid; To radiocystitis, 0.4g total solid associating antibiotic therapy on the one, per urethra administration.
Embodiment 3:
Deproteinised calf blood extract for injection and its prepration technology, its composition comprises: calf blood protein-removed extraction, described calf blood protein-removed extraction is to extract from fresh calf blood or serum, described calf blood protein-removed extraction solid content contains total nitrogen, free amino acid, its ratio of weight and number is a total nitrogen 0.50, free amino acid 0.20, pH value should be 5.0, molecular weight is 7000, the reaction of indenes three bronze medals is positive, its preparation technology is: calf blood protein-removed extraction solution is put into freeze drying box, goods are refrigerated to-40 ℃, cold-trap drops to-40 ℃, evacuation makes drying baker vacuum reach 7Pa, during primary drying, the conduction oil temperature is set in-15 ℃, goods are sublimation drying at the uniform velocity, during redrying, the conduction oil temperature is set in 35 ℃, product dried, when products temperature reaches 33 ℃, continue dry until up to specification, it is off-white color or faint yellow block or amorphous powder that the goods bone dry obtains outward appearance, make aseptic freeze-dried product, and press 100mg, 200mg, 400mg, 600mg and 800mg specification packing can be used for intravenous injection, intra-arterial injection, intramuscular injection, also can add 200ml 5% glucose or 0.9% sodium chloride injection iv drip, the about 2ml/min of drip velocity is to brain ischemic lesions (apoplexy and cerebral trauma), the intravenous drip of a 1.0g total solid, once-a-day, two weeks were a course of treatment; Brain function is not reached brain dementia entirely, the intravenous injection of a 1.2g total solid, 1 time on the one, two weeks were a course of treatment; To artery vessel disease, the intravenous drip of a 1.5g total solid, 1 time on the one or 1.5g total solid tremulous pulse or intravenous injection, weekly for several times, course of treatment all around; To shank or other chronic ulcers, burn, each quiet notes of 0.4g total solid (or the intramuscular injection of 0.2g total solid) 1 time on the one or weekly for several times, can add by healing state and to use this product topical therapeutic; To the prevention and the treatment of radioactive skin, mucosa injury, at radiation period, the average one day quiet notes of 0.2g total solid; To radiocystitis, 0.4g total solid associating antibiotic therapy on the one, per urethra administration.
Embodiment 4:
The preparation technology of above-mentioned calf blood protein-removed extraction solution, it is characterized in that: get above-mentioned calf blood or calf serum, acid adjustment pH value to 3.0, transfer alkaline pH value to 8.0 again, after the accent neutrality, be heated to 30 ℃, go precipitation, use the ultrafilter membrane ultrafiltration then, the molecular weight that dams is 5000 dalton, collects filtrate and is calf blood or calf serum protein-removing extract solution.
Embodiment 5:
The preparation technology of above-mentioned calf blood protein-removed extraction solution, it is characterized in that: get above-mentioned calf blood or calf serum, acid adjustment pH value to 5.5, transfer alkaline pH value to 10.5 again, after the accent neutrality, be heated to 80 ℃, go precipitation, use the ultrafilter membrane ultrafiltration then, the molecular weight that dams is 10000 dalton, collects filtrate and is calf blood or calf serum protein-removing extract solution.
Embodiment 6:
The preparation technology of above-mentioned calf blood protein-removed extraction solution, get above-mentioned calf blood or calf serum, acid adjustment pH value to 4.5, transfer alkaline pH value to 9.5 again, after the accent neutrality, be heated to 60 ℃, go precipitation, use the ultrafilter membrane ultrafiltration then, the molecular weight that dams is 8000 dalton, collects filtrate and is calf blood or calf serum protein-removing extract solution.
Embodiment 7:
Deproteinised calf blood extract for injection and its prepration technology, its composition comprises: calf blood protein-removed extraction, described calf blood protein-removed extraction is to extract from fresh calf blood or serum, described calf blood protein-removed extraction solid content contains total nitrogen, free amino acid, its ratio of weight and number is a total nitrogen 0.10, free amino acid 0.01, pH value should be 3.0, molecular weight is 5000, the reaction of indenes three bronze medals is positive, its preparation technology is: remove fiber calf blood or serum, add an amount of 3M HCl solution, acid adjustment pH value to 3.0 adds an amount of 3M NaOH solution again, transfer alkaline pH value to 8.0, get supernatant, add an amount of 3M HCl solution, transfer pH neutral, get neutral solution, be heated to 30 ℃ of heat treatments 5 minutes, and went precipitation, filtrate is 5000 dalton's ultrafiltration with ultrafilter membrane relative molecular weight cutoff value, collect filtrate and promptly get calf blood protein-removed extraction solution, it is an amount of to get calf blood protein-removed extraction solution, puts into freeze drying box, and goods are refrigerated to-45 ℃, cold-trap drops to-45 ℃, evacuation makes drying baker vacuum reach 5Pa, during primary drying, the conduction oil temperature is at-5 ℃, and goods are sublimation drying at the uniform velocity; During redrying, the conduction oil temperature is set in 30 ℃, and product dried is when products temperature reaches 35 ℃, continued dry 1 hour, obtaining outward appearance is off-white color or faint yellow block or amorphous powder, promptly gets injection calf blood protein-removed extraction of the present invention, and packing specification has 100mg, 200mg, 400mg, 600mg and 800mg, to brain ischemic lesions (apoplexy and cerebral trauma), the intravenous drip of a 0.8g total solid, once-a-day, two weeks were a course of treatment; Brain function is not reached brain dementia entirely, the intravenous injection of a 1.2g total solid, 1 time on the one, two weeks were a course of treatment; To artery vessel disease, the intravenous drip of a 1.5g total solid, 1 time on the one or 1.5g total solid tremulous pulse or intravenous injection, weekly for several times, course of treatment all around; To shank or other chronic ulcers, burn, each quiet notes of 0.4g total solid (or the intramuscular injection of 0.2g total solid) 1 time on the one or weekly for several times, can add by healing state and to use this product topical therapeutic; To the prevention and the treatment of radioactive skin, mucosa injury, at radiation period, the average one day quiet notes of 0.2g total solid; To radiocystitis, 0.4g total solid associating antibiotic therapy on the one, per urethra administration.
Embodiment 8:
Deproteinised calf blood extract for injection and its prepration technology, its composition comprises: calf blood protein-removed extraction, described calf blood protein-removed extraction is to extract from fresh calf blood or serum, described calf blood protein-removed extraction solid content contains total nitrogen, free amino acid, its ratio of weight and number is a total nitrogen 0.90, free amino acid 0.40, pH value should be 9.0, molecular weight is 10000, the reaction of indenes three bronze medals is positive, its preparation technology is: remove fiber calf blood or serum, add an amount of 3M HCl solution, acid adjustment pH value to 5.5 adds 3M NaOH solution again, transfer alkaline pH value to 10.5, get supernatant, add 3MHCl solution, transfer pH neutral, get neutral solution, be heated to 80 ℃ of heat treatments 5 minutes, and went precipitation, filtrate is 10000 dalton's ultrafiltration with ultrafilter membrane relative molecular weight cutoff value, collect filtrate and promptly get calf blood protein-removed extraction solution, it is an amount of to get calf blood protein-removed extraction solution, puts into freeze drying box, and goods are refrigerated to-45 ℃, cold-trap drops to-45 ℃, evacuation makes drying baker vacuum reach 5Pa, during primary drying, the conduction oil temperature is at-5 ℃, and goods are sublimation drying at the uniform velocity; During redrying, the conduction oil temperature is set in 30 ℃, and product dried is when products temperature reaches 35 ℃, continued dry 1 hour, obtaining outward appearance is off-white color or faint yellow block or amorphous powder, promptly gets injection calf blood protein-removed extraction of the present invention, and packing specification has 100mg, 200mg, 400mg, 600mg and 800mg, to brain ischemic lesions (apoplexy and cerebral trauma), the intravenous drip of a 1.2g total solid, once-a-day, two weeks were a course of treatment; Brain function is not reached brain dementia entirely, the intravenous injection of a 1.2g total solid, 1 time on the one, two weeks were a course of treatment; To artery vessel disease, the intravenous drip of a 1.5g total solid, 1 time on the one or 1.5g total solid tremulous pulse or intravenous injection, weekly for several times, course of treatment all around; To shank or other chronic ulcers, burn, each quiet notes of 0.4g total solid (or the intramuscular injection of 0.2g total solid) 1 time on the one or weekly for several times, can add by healing state and to use this product topical therapeutic; To the prevention and the treatment of radioactive skin, mucosa injury, at radiation period, the average one day quiet notes of 0.2g total solid; To radiocystitis, 0.4g total solid associating antibiotic therapy on the one, per urethra administration.
Embodiment 9:
Deproteinised calf blood extract for injection and its prepration technology, its composition comprises: calf blood protein-removed extraction, described calf blood protein-removed extraction is to extract from fresh calf blood or serum, described calf blood protein-removed extraction solid content contains total nitrogen, free amino acid, its ratio of weight and number is a total nitrogen 0.40, free amino acid 0.20, pH value should be 6.0, molecular weight is 8000, the reaction of indenes three bronze medals is positive, its preparation technology is: remove fiber calf blood or serum, add 3M HCl solution, acid adjustment pH value to 4.0 adds 3M NaOH solution again, transfer alkaline pH value to 9.0, get supernatant, add an amount of 3M HCl solution, transfer pH neutral, get neutral solution, be heated to 60 ℃ of heat treatments 5 minutes, and went precipitation, filtrate is 8000 dalton's ultrafiltration with ultrafilter membrane relative molecular weight cutoff value, collect filtrate and promptly get calf blood protein-removed extraction solution, it is an amount of to get calf blood protein-removed extraction solution, puts into freeze drying box, and goods are refrigerated to-45 ℃, cold-trap drops to-45 ℃, evacuation makes drying baker vacuum reach 8Pa, during primary drying, the conduction oil temperature is at-5 ℃, and goods are sublimation drying at the uniform velocity; During redrying, the conduction oil temperature is set in 30 ℃, and product dried is when products temperature reaches 35 ℃, continued dry 1 hour, obtaining outward appearance is off-white color or faint yellow block or amorphous powder, promptly gets injection calf blood protein-removed extraction of the present invention, and packing specification has 100mg, 200mg, 400mg, 600mg and 800mg, to brain ischemic lesions (apoplexy and cerebral trauma), the intravenous drip of a 1.0g total solid, once-a-day, two weeks were a course of treatment; Brain function is not reached brain dementia entirely, the intravenous injection of a 1.2g total solid, 1 time on the one, two weeks were a course of treatment; To artery vessel disease, the intravenous drip of a 1.5g total solid, 1 time on the one or 1.5g total solid tremulous pulse or intravenous injection, weekly for several times, course of treatment all around; To shank or other chronic ulcers, burn, each quiet notes of 0.4g total solid (or the intramuscular injection of 0.2g total solid) 1 time on the one or weekly for several times, can add by healing state and to use this product topical therapeutic; To the prevention and the treatment of radioactive skin, mucosa injury, at radiation period, the average one day quiet notes of 0.2g total solid; To radiocystitis, 0.4g total solid associating antibiotic therapy on the one, per urethra administration.
Claims (3)
1. the preparation technology of an injection calf blood protein-removed extraction, its composition comprises: calf blood protein-removed extraction, described calf blood protein-removed extraction is to extract from fresh calf blood or serum, described calf blood protein-removed extraction solid content contains total nitrogen, free amino acid, its ratio of weight and number is a total nitrogen 0.10, free amino acid 0.01, pH value should be 3.0, molecular weight is 5000, and the reaction of indenes three bronze medals is positive, it is characterized by: described calf blood protein-removed extraction solution is put into freeze drying box, goods are refrigerated to-45 ℃～20 ℃, cold-trap drops to-45 ℃～20 ℃, and evacuation makes drying baker vacuum reach 1～10Pa, during primary drying, the conduction oil temperature is set in-5 ℃～20 ℃, goods are sublimation drying at the uniform velocity, and during redrying, the conduction oil temperature is set in 30～35 ℃, product dried, when products temperature reached 35 ℃, it was off-white color or faint yellow block or amorphous powder that the dry straight goods bone dry of continuation obtains outward appearance, makes aseptic freeze-dried product.
2. the preparation technology of injection calf blood protein-removed extraction according to claim 1, it is characterized in that: get above-mentioned calf blood or calf serum, acid adjustment pH value to 3.0～5.5, transfer alkaline pH value to 8.0～10.5 again, after the accent neutrality, be heated to 30～80 ℃, go precipitation, use the ultrafilter membrane ultrafiltration then, the molecular weight that dams is 5000～10000 dalton, collects filtrate and is calf blood or calf serum protein-removing extract solution.
3. injection calf blood protein-removed extraction according to claim 1 and 2, its composition comprises: calf blood protein-removed extraction, it is characterized in that: described calf blood protein-removed extraction is to extract from fresh calf blood or serum, described calf blood protein-removed extraction solid content contains total ammonia, free amino acid, its ratio of weight and number is a total nitrogen 0.10～0.90, free amino acid 0.01～0.40, and pH value should be 3.0～9.0, molecular weight is 5000～10000, and the reaction of indenes three bronze medals is positive.
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2004100135096A CN100352450C (en) | 2004-01-12 | 2004-01-12 | Albumen removed extraction of calf blood for injection and its preparing process |
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2004100135096A CN100352450C (en) | 2004-01-12 | 2004-01-12 | Albumen removed extraction of calf blood for injection and its preparing process |
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| CN1569030A CN1569030A (en) | 2005-01-26 |
| CN100352450C true CN100352450C (en) | 2007-12-05 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN100396291C (en) * | 2005-06-09 | 2008-06-25 | 赵红梅 | Injection of calf blood de-protein extract and preparation method thereof |
| CN101433553B (en) * | 2007-12-07 | 2011-08-17 | 吉林康乃尔药业有限公司 | Method for preparing deproteinized extract of calf blood and freeze-dried powder thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1183280A (en) * | 1996-11-27 | 1998-06-03 | 锦州医学院科技开发部 | Method for extracting deproteinized calf blood extract |
| CN1337403A (en) * | 2000-08-04 | 2002-02-27 | 中国药品生物制品检定所 | Active monopeptide prepared from active blood polypeptide and its prepn. activity and sequence |
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2004
- 2004-01-12 CN CNB2004100135096A patent/CN100352450C/en not_active Expired - Lifetime
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1183280A (en) * | 1996-11-27 | 1998-06-03 | 锦州医学院科技开发部 | Method for extracting deproteinized calf blood extract |
| CN1337403A (en) * | 2000-08-04 | 2002-02-27 | 中国药品生物制品检定所 | Active monopeptide prepared from active blood polypeptide and its prepn. activity and sequence |
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| CN1569030A (en) | 2005-01-26 |
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Assignee: Heilongjiang Jiang Shi Pharmaceutical Co.,Ltd. Assignor: Jiang Yong Contract fulfillment period: 2008.8.18 to 2018.8.18 Contract record no.: 2008230000023 Denomination of invention: Albumen removed extraction of calf blood for injection and its preparing process Granted publication date: 20071205 License type: Exclusive license Record date: 20081009 |
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