CN100342924C - Skin substitute and its preparation method - Google Patents
Skin substitute and its preparation method Download PDFInfo
- Publication number
- CN100342924C CN100342924C CNB2004100649884A CN200410064988A CN100342924C CN 100342924 C CN100342924 C CN 100342924C CN B2004100649884 A CNB2004100649884 A CN B2004100649884A CN 200410064988 A CN200410064988 A CN 200410064988A CN 100342924 C CN100342924 C CN 100342924C
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- fibroin
- film
- skin
- graftskin
- polymeric membrane
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- 108010022355 Fibroins Proteins 0.000 claims abstract description 77
- 239000012528 membrane Substances 0.000 claims abstract description 26
- 239000011148 porous material Substances 0.000 claims abstract description 19
- 150000001875 compounds Chemical class 0.000 claims abstract description 14
- 241000255789 Bombyx mori Species 0.000 claims description 27
- 239000000243 solution Substances 0.000 claims description 21
- 239000007864 aqueous solution Substances 0.000 claims description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- 108010013296 Sericins Proteins 0.000 claims description 13
- 239000000654 additive Substances 0.000 claims description 13
- 230000000996 additive effect Effects 0.000 claims description 13
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 11
- 238000012545 processing Methods 0.000 claims description 9
- 238000011282 treatment Methods 0.000 claims description 9
- 238000000034 method Methods 0.000 claims description 8
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- 239000002994 raw material Substances 0.000 claims description 2
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- 208000027418 Wounds and injury Diseases 0.000 abstract description 12
- 239000000463 material Substances 0.000 abstract description 11
- 210000004207 dermis Anatomy 0.000 abstract description 3
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- 239000004372 Polyvinyl alcohol Substances 0.000 description 8
- 229920002451 polyvinyl alcohol Polymers 0.000 description 8
- 239000003292 glue Substances 0.000 description 7
- 229920006264 polyurethane film Polymers 0.000 description 7
- 239000008367 deionised water Substances 0.000 description 6
- 229910021641 deionized water Inorganic materials 0.000 description 6
- 239000000835 fiber Substances 0.000 description 6
- 238000004108 freeze drying Methods 0.000 description 6
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- 102000008186 Collagen Human genes 0.000 description 4
- 108010035532 Collagen Proteins 0.000 description 4
- 238000001035 drying Methods 0.000 description 4
- 238000001914 filtration Methods 0.000 description 4
- AMXOYNBUYSYVKV-UHFFFAOYSA-M lithium bromide Chemical compound [Li+].[Br-] AMXOYNBUYSYVKV-UHFFFAOYSA-M 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
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- 238000005516 engineering process Methods 0.000 description 3
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- 241000255791 Bombyx Species 0.000 description 2
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 2
- 229920001661 Chitosan Polymers 0.000 description 2
- 206010013786 Dry skin Diseases 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
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- 241000382353 Pupa Species 0.000 description 2
- 208000028990 Skin injury Diseases 0.000 description 2
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
- 241000282894 Sus scrofa domesticus Species 0.000 description 2
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- 239000002250 absorbent Substances 0.000 description 2
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- 239000001110 calcium chloride Substances 0.000 description 2
- 229910001628 calcium chloride Inorganic materials 0.000 description 2
- ZCCIPPOKBCJFDN-UHFFFAOYSA-N calcium nitrate Chemical compound [Ca+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ZCCIPPOKBCJFDN-UHFFFAOYSA-N 0.000 description 2
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- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- WHXSMMKQMYFTQS-UHFFFAOYSA-N Lithium Chemical compound [Li] WHXSMMKQMYFTQS-UHFFFAOYSA-N 0.000 description 1
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- Materials For Medical Uses (AREA)
Abstract
The present invention belongs to the field of biomedical materials, which particularly relates to a skin substitute that is applicable to treat burns, wounds, plasty, etc. and a preparation method thereof. The skin substitute is formed by compounding fibroin porous materials of a lower layer, a fibroin membrane of a middle layer and a macromolecular membrane of an upper layer. The macromolecular membrane is tight bonded with the fibroin membrane after being processed by low-temperature plasma, and forms favorable compound with the fibroin porous materials. Capillary vessels and dermis cells can be induced to grow into the skin substitute by the skin substitute so as to realize the regeneration and the repair of dermis tissues and have favorable biocompatibility, and the coverage by autologous skin, allogeneic skin or xenogeneic skin is not needed when the skin substitute is transplanted.
Description
Technical field
The invention belongs to field of biomedical materials, the treatment of particularly a kind of burn that is applicable to dark II degree and III degree, wound, cold injury, ulcer etc. and Graftskin of shaping and preparation method thereof.
Background technology
Skin is the organ of human body maximum, is made up of epidermis, corium, subcutaneous tissue and cutaneous appendage.Often, when damage relates to corium, generally be difficult to recover original form of skin and function because of reasons such as physics, chemistry, biology cause skin injury.The means that treatment corium is damaged are also few at present, mainly are to transplant tomography from the body skin graft, and distinct issues are still the scar hyperplasia that is subjected to the dermatotome, influence outward appearance and function.
Another means of treatment skin injury are to cover with Graftskin.The Graftskin of Chu Xianing (claiming " wound-surface cover " or " artificial skin " sometimes) has two classes in the market.One class is temporary, for example regenerated silk film, chitosan film, polyurethane film, tomography Corii Sus domestica etc.; Another kind of is nonvolatil, for example takes off cell allosome corium, takes off the cell Corii Sus domestica, collagen protein, chitosan porous material etc.Temporary Graftskin generally can prevent a large amount of losses of body fluid and bacteria attack to a certain extent, need throw off or come off naturally behind the wound healing, generally is only applicable to the skin blemish wound surface that superficial burns or other reasons cause; Nonvolatil Graftskin generally can make corium form and function obtain repairing to a certain extent, and is biodegradable, so generally be used for the damaged wound surface of corium that deep burn or other reasons cause.
Before the present invention made, domestic and international many researcheres once were devoted to the research of permanent Graftskin, had obtained many progress.For example, publication number is the technical scheme of the Chinese patent " cross-linking type acellular pork skin " of CN 1266716, be to scrub unhairing after selecting white pig to live extremely, strip full pachydermia piece, the anti-thick skin graft of about 0.1-0.4mm of getting after the degrease in compound Digestive systems such as 0.25% trypsin 4 ℃, 16～24 hours, is thrown off epidermis, the netted punching of corium back is crosslinked with glutaraldehyde or formaldehyde-alcohol mixed solution, and obtaining is used to burn cuts/cut the dermis graft of crust wound surface and shaping patient's the wound repair of cutting scar.By publication number is the technology that the Chinese patent " medical collagen sponge and preparation method thereof " of CN1416907 is adopted, impurity is removed by the animal tissue of being rich in collagen protein to be pulverized, after dilute alkaline soln and alcohols material washing defat, dissolving, Protease Treatment, precipitation, acetic acid dissolving, lyophilization, fixing again, obtain being used for wound repair and hemostatic medical collagen sponge.
But the existing Graftskin that is used for the damaged wound surface of corium that deep burn or other reasons cause, the problem that solves of still needing is: (1) often needs the autologous transplanting skin or covers with allosome, xenogenesis skin when transplanting this type of Graftskin, otherwise easily make it withered because of hydropenia, but Graftskin this moment not vascularization as yet, the survival rate from the body surface skin of transplanting is affected, and disposable skin donor site deficiency during large-area burns; (2) take off cell allosome or xenogenesis corium and can remove the virus that allosome (or xenogenesis) substrate is carried fully, can avoid transmission of disease fully, wait to observe; (3) collagen protein of particular type just can be used for preparing nonvolatil Graftskin, but the purifying technique of particular type collagen protein is more loaded down with trivial details, and the manufacturing cost of substitute still has necessity of reduction.
Notification number is the patent of invention " a kind of porous fibroin membrane and preparation method thereof " of CN1118518C, adopt technically be with silkworm silk through come unstuck, dissolve, dialyse, concentrated after, adding contains 10～60% R (OH) n, 10～60% RCH (NH
2) COOH, all the other are the additive of cross-linking agent, are processed into porous fibroin membrane through freeze-drying again, can be used as wound surface covering and artificial skin material, drug controlled release material, anticoagulant material etc.But when being used for the reparation of the damaged wound surface of corium that deep burn or other reasons cause, when transplanting porous fibroin membrane, still need using from the body skin or with allosome, xenogenesis skin to cover, otherwise easily dry up because of hydropenia makes porous fibroin membrane.
Summary of the invention
Purpose of the present invention is exactly the deficiency that exists at prior art, and a kind of excellent biological compatibility that possesses is provided, and realizes multiple layer Graftskin of the damaged wound repair of corium and preparation method thereof.
For achieving the above object, the technical solution used in the present invention is: a kind of Graftskin is that the fibroin film of upper strata, thickness 0.05～0.5mm is that the fibroin porous material of middle level, thickness 0.1～1.5mm is that lower floor is composited by the polymeric membrane of thickness 0.1～1.0mm.
In the technique scheme, described upper strata polymeric membrane can be the dense film of natural or synthetic high polymer such as bombyx mori silk fibroin, tussah silk peptide, polyurethane, silicone rubber, polyvinyl alcohol; Described middle level fibroin film is a dense film, wherein contains 0～90% bombyx mori silk fibroin, 0～90% tussah silk peptide and 0～90% silkworm silk sericin, and all the other are additive, and the material in middle level is formed and ratio can be identical or different with lower floor; Contain 0～90% bombyx mori silk fibroin, 0～90% tussah silk peptide and 0～90% silkworm silk sericin in the described lower floor fibroin porous material, contain a kind of in the above-mentioned solute at least, all the other are additive.The kind of additive and adding mode are prior art, for example, promptly disclose a kind of technical scheme that adds additive among the Chinese invention patent CN1118518C " a kind of porous fibroin membrane and preparation method thereof ".
The preparation method of Graftskin of the present invention, it is a raw material with in silkworm sericin, bombyx mori silk fibroin, the tussah silk peptide solution one or more, adopt cryodesiccated method to be prepared into the fibroin porous material after adding additive, it is characterized in that carrying out again the processing of following steps:
(1) be He, Ar, CO, NH with polymeric membrane at working gas
3, O
2, H
2In a kind of condition under carry out Low Temperature Plasma Treating, the processing time is 0.5～10 minute;
(2) the preparation total concentration is 0.1～20% compound aqueous solution, and wherein the relative scale of various solutes is: 0～90% bombyx mori silk fibroin, 0～90% tussah silk peptide, 0～90% silkworm silk sericin, contain a kind of in the above-mentioned solute at least, and all the other are additive;
(3) polymeric membrane after the Low Temperature Plasma Treating be impregnated in the above-mentioned compound aqueous solution immediately, solution temperature is 10～90 ℃, dip time is more than 0.5 minute, and then to be impregnated in temperature be in 10～90 ℃ the water, oscillation treatment is more than 0.5 hour, be dry under 20～100 ℃ the condition in temperature, on polymeric membrane, be compounded to form the fibroin film;
(4) compound aqueous solution evenly is applied in the surface of fibroin film, the fibroin porous material is covered on it, dried is 10～60 minutes under 20～100 ℃ temperature conditions, obtains the Graftskin that is composited by upper strata polymeric membrane, middle level fibroin film, lower floor's fibroin porous material.
In the technique scheme,, can carry out step (4) before, step (1) to (3) is being repeated 1～3 time, on polymeric membrane, be compounded to form the fibroin film for guaranteeing the thickness and the compactness in middle level.
Compared with prior art, Graftskin of the present invention has following remarkable advantage:
1, because its material of main part of Graftskin provided by the present invention is a fibroin, the native protein that fibroin is made up of 18 seed amino acids, has excellent biological compatibility, therefore, the Graftskin that makes has been avoided the virus that may carry from vertebra class animal material, antigenic influence and transmission of disease.
2, Graftskin of the present invention adopts the multilayer structure technology of different materials, after it is covered wound surface, lower floor can induce new vessels to grow into, the regeneration of guiding hypodermal cell, tissue, be the support of corium to regenerate, the fibroin porous material progressively is degraded in corium form and function repair process; The upper strata prevents that antibacterial from invading and body fluid runs off, and is that good environment is built in the reparation of corium form and function, possesses the good mechanical performance owing to the upper strata simultaneously, can prevent art before, in the art, the breakage of postoperative skin substitute; Treat that blood vessel is grown into and throw off upper strata and middle level after the lower floor of Graftskin, only need in its surface transplantation, cover from body sword pachydermia (thin skin), can reach the purpose that deep burn wound is repaired, not need in the cutify substitute, just to use from the body skin or with allosome, xenogenesis skin to cover; The middle level is fine and close fibroin film, possesses the good cell compatibility, and it cooperates lower floor to make the form behind the wound healing good, helps recovering original form of skin and function.
3, owing to adopted plasma repeated treatments method, polymeric membranes such as the fibroin mass-energy such as middle level bombyx mori silk fibroin, tussah silk peptide, sericin of Graftskin and upper strata fibroin, polyurethane, silicone rubber, polyvinyl alcohol are combined closely, help the formation of the protein dense film in middle level, thereby realize substituting the function of skin.
4, the fibroin albumen source is abundant, purification is easy, can avoid complicated, loaded down with trivial details processing technique, accomplishes scale production easily, and low cost of manufacture has broad application prospects.
The specific embodiment
Below in conjunction with embodiment the present invention is further described:
Embodiment one:
1, tame Bombyx bombycis is through shelling cocoon, sectility, going pupa to obtain cocoon layer, behind the silkworm cocoon layer usefulness tap water thorough washing with 1 kilogram of cleaning, reuse deionized water wash 3 times, put into 20 liters of deionized waters, made the sericin dissolving in 40 minutes in 98～100 ℃ of processing, obtain the sericin aqueous solution after the filtration, the sericin aqueous solution obtains solid sericin after lyophilization.Take by weighing 10g solid filament glue and be dissolved in and be mixed with 1000ml in the deionized water, obtain concentration and be 1% domestic silkworm silk glue protein solution.
2,1 kilogram of domestic silkworm silk being put into 10 liters of concentration is 0.025% aqueous sodium carbonate, boils 0.5 hour, and the sericin of most silkworm silk periphery is taken off in reprocessing three times, obtains the bombyx mori silk fibroin fiber; Fibroin fiber after will drying is 1: 8: 2 calcium chloride, water, an alcoholic solution with 10 liters of mol ratios, becomes the fibroin albumen mixed solution 70 ± 2 ℃ of following heating for dissolving; With cellulose membrane is the dialysis material, the fibroin albumen mixed solution water of gained is dialysed, and with removal calcium chloride, impurity such as ethanol, reuse multilamellar absorbent carbasus filters, and obtains silk fibroin protein solution, regulator solution concentration to 5%.
3, with concentration be the lithium rhodanate of 9M, in 40 ℃ of temperature, the tussah silk peptide fiber that bath raio dissolving in 1: 20 has been come unstuck, obtain mixed solution, above-mentioned solution is dialysed to water, obtain tussah silk fibroin solution after the filtration, the concentration adjustment to 5% of tussah silk peptide solution.
4, silk fibroin protein solution is mixed by 10: 90 volume ratio with domestic silkworm silk glue protein solution, be that the technical scheme described in the patent of invention " a kind of porous fibroin membrane and preparation method thereof " of CN1118518C adds additive by notification number, adopt freeze drying process to make the fibroin porous material that thickness is 0.4mm.
5, silk fibroin protein solution is mixed according to 70: 30 volume ratio with tussah silk fibroin solution, add additive, make concentration and be 5% compound aqueous solution.
6, preparation thickness is the polyurethane film of 0.5mm; equally; also can adopt other synthesized polymer material film, also can adopt by publication number is the fibroin protein film that obtains of the technical scheme in the Chinese patent " a kind of Wound surface protection film and preparation method thereof " of CN1121836A or other natural polymer film etc.
7, polyurethane film is carried out Low Temperature Plasma Treating, working gas is an Ar gas, and the processing time is 0.5 minute.
8, will impregnated in through the polyurethane film after step 7 is handled immediately in the compound aqueous solution that step 5 makes, solution temperature is 20 ℃, and dip time is 20 minutes.
9, will be in temperature be 25 ℃ deionized water through the film immersion after step 8 is handled, oscillation treatment 3 hours was changed a water every 1 hour.
10, in 35 ℃ of dryings.
11, step 7～10 repeat 3 times, form the fibroin film in the polyurethane film surface recombination.
12, the compound aqueous solution that step 5 is made is evenly smeared the film surface that above-mentioned polyurethane film and fibroin film are composited, and the fibroin porous material that step 4 is made is covered on the film.
13, in 35 ℃ of dryings, obtaining the upper strata is that polyurethane film, middle level are the Graftskin that fibroin film, lower floor are composited for the fibroin porous material, after cutting, packing, uses Co
60Irradiation, irradiation dose are 25kGry.
Embodiment two:
1, tame Bombyx bombycis is through shelling cocoon, sectility, going pupa to obtain cocoon layer, behind the silkworm cocoon layer usefulness tap water thorough washing with 1 kilogram of cleaning, reuse deionized water wash 3 times, put into 20 liters of deionized waters, made the sericin dissolving in 40 minutes in 98～100 ℃ of processing, obtain the sericin aqueous solution after the filtration, the sericin aqueous solution obtains solid sericin after lyophilization.Take by weighing 10g solid filament glue and be dissolved in and be mixed with 1000ml in the deionized water, obtain concentration and be 1% domestic silkworm silk glue protein solution.
2,0.5 kilogram of silkworm cocoon layer being put into 20 liters of concentration is 0.4% neutral soap solution, handles 2 hours down in 98～100 ℃ of temperature conditions, takes off the sericin of most silkworm silk periphery, thorough washing, obtains fibroin fiber after drying; With the lithium-bromide solution of 28 liter of 8.5 mol,, obtain the fibroin albumen mixed solution in 60 ± 2 ℃ of stirring and dissolving fibroin fibers; Impurity such as lithium bromide are removed in the water dialysis, and reuse multilamellar absorbent carbasus filters, and obtains silk fibroin protein solution, regulator solution concentration to 5%.
3, use fused calcium nitrate solution, in 105 ℃ of temperature, the tussah silk peptide fiber that bath raio dissolving in 1: 20 has been come unstuck obtains mixed solution, and above-mentioned solution is dialysed to water, obtains tussah silk fibroin solution after the filtration, regulator solution concentration to 5%.
4, silk fibroin protein solution, domestic silkworm silk glue protein solution and tussah silk fibroin solution are pressed 80: the volume ratio of 1O: 1O is mixed, and behind the adding additive, adopts freeze-drying to make the fibroin porous material that thickness is 0.9mm.
5, silk fibroin protein solution, domestic silkworm silk glue protein solution and tussah silk fibroin solution are pressed 80: 1O: 10 volume ratio is mixed, and behind the adding additive, makes compound aqueous solution.
6, preparation thickness is the polyvinyl alcohol film of 0.8mm.
7, polyvinyl alcohol film is carried out Low Temperature Plasma Treating, working gas is O
2Gas, the processing time is 1 minute.
8, will impregnated in through the polyvinyl alcohol film after step 7 is handled immediately in the compound aqueous solution that step 5 makes, solution temperature is 60 ℃, and dip time is 60 minutes.
9, will be in temperature be 60 ℃ deionized water through the film immersion after step 8 is handled, oscillation treatment 24 hours was changed a water every 4 hours.
10, dry under 60 ℃ temperature, on polyvinyl alcohol film, be compounded to form the fibroin film.
11, the compound aqueous solution that step 5 is made evenly is applied in the film surface that above-mentioned polyvinyl alcohol film and fibroin film are composited, and the fibroin porous material that step 4 is made covers on it.
12, dry under 60 ℃ temperature, obtaining the upper strata is that polyvinyl alcohol film, middle level are the Graftskin that fibroin film, lower floor are composited for the fibroin porous material, after cutting, packing, uses Co
60Irradiation, irradiation dose are 15kGry.
Claims (3)
1. Graftskin, it is characterized in that: it is that polymeric membrane by thickness 0.1～1.0mm is that the fibroin film of upper strata, thickness 0.05～0.5mm is that the fibroin porous material of middle level, thickness 0.1～1.5mm is that lower floor is composited, and described upper strata polymeric membrane and middle level fibroin film are dense film.
2. the preparation method of a Graftskin, it is a raw material with in silkworm sericin, bombyx mori silk fibroin, the tussah silk peptide solution one or more, adopt cryodesiccated method to be prepared into the fibroin porous material after adding additive, it is characterized in that carrying out again the processing of following steps:
(1) be He, Ar, CO, NH with polymeric membrane at working gas
3, O
2, H
2In a kind of condition under carry out Low Temperature Plasma Treating, the processing time is 0.5～10 minute;
(2) the preparation total concentration is 0.1～20% compound aqueous solution, and wherein the relative scale of various solutes is: 0～90% bombyx mori silk fibroin, 0～90% tussah silk peptide, 0～90% silkworm silk sericin, contain a kind of in the above-mentioned solute at least, and all the other are additive;
(3) polymeric membrane after the Low Temperature Plasma Treating be impregnated in the above-mentioned compound aqueous solution immediately, solution temperature is 10～90 ℃, dip time is more than 0.5 minute, and then to be impregnated in temperature be in 10～90 ℃ the water, oscillation treatment is more than 0.5 hour, be dry under 20～100 ℃ the condition in temperature, on polymeric membrane, be compounded to form the fibroin film;
(4) compound aqueous solution evenly is applied in the surface of fibroin film, the fibroin porous material is covered on it, dried is 10～60 minutes under 20～100 ℃ temperature conditions, obtains the Graftskin that is composited by upper strata polymeric membrane, middle level fibroin film, lower floor's fibroin porous material.
3. the preparation method of Graftskin according to claim 2 is characterized in that: carrying out step (4) before, step (1) to (3) is repeated 1～3 time, be compounded to form the fibroin film on polymeric membrane.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100649884A CN100342924C (en) | 2004-10-13 | 2004-10-13 | Skin substitute and its preparation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100649884A CN100342924C (en) | 2004-10-13 | 2004-10-13 | Skin substitute and its preparation method |
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| CN1628864A CN1628864A (en) | 2005-06-22 |
| CN100342924C true CN100342924C (en) | 2007-10-17 |
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| Application Number | Title | Priority Date | Filing Date |
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| CNB2004100649884A Expired - Lifetime CN100342924C (en) | 2004-10-13 | 2004-10-13 | Skin substitute and its preparation method |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01254164A (en) * | 1988-04-01 | 1989-10-11 | Terumo Corp | Wound protecting material and production thereof |
| CN1260363A (en) * | 2000-02-11 | 2000-07-19 | 苏州大学 | Porous fibroin membrane and its preparation method |
| CN1348820A (en) * | 2001-11-10 | 2002-05-15 | 苏州大学 | Coremia-resisting wound protecting film and its prepn |
| CN1397355A (en) * | 2002-08-14 | 2003-02-19 | 苏州大学 | Material for repairing defective tissue of body and its preparing process |
| CN1397654A (en) * | 2002-05-27 | 2003-02-19 | 长沙力元新材料股份有限公司 | Process and apparatus for preparing porous metal by combined physical gas-phase deposition techinque |
| CN1155411C (en) * | 2001-11-16 | 2004-06-30 | 崔翔 | Freezing absorbant hemostatic cotton and its prepn |
| WO2004062697A2 (en) * | 2003-01-07 | 2004-07-29 | Tufts University | Silk fibroin materials and use thereof |
-
2004
- 2004-10-13 CN CNB2004100649884A patent/CN100342924C/en not_active Expired - Lifetime
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01254164A (en) * | 1988-04-01 | 1989-10-11 | Terumo Corp | Wound protecting material and production thereof |
| CN1260363A (en) * | 2000-02-11 | 2000-07-19 | 苏州大学 | Porous fibroin membrane and its preparation method |
| CN1348820A (en) * | 2001-11-10 | 2002-05-15 | 苏州大学 | Coremia-resisting wound protecting film and its prepn |
| CN1155411C (en) * | 2001-11-16 | 2004-06-30 | 崔翔 | Freezing absorbant hemostatic cotton and its prepn |
| CN1397654A (en) * | 2002-05-27 | 2003-02-19 | 长沙力元新材料股份有限公司 | Process and apparatus for preparing porous metal by combined physical gas-phase deposition techinque |
| CN1397355A (en) * | 2002-08-14 | 2003-02-19 | 苏州大学 | Material for repairing defective tissue of body and its preparing process |
| WO2004062697A2 (en) * | 2003-01-07 | 2004-07-29 | Tufts University | Silk fibroin materials and use thereof |
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| CN1628864A (en) | 2005-06-22 |
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