CN100336565C - Plant source protein three-dimensional stent material - Google Patents
Plant source protein three-dimensional stent material Download PDFInfo
- Publication number
- CN100336565C CN100336565C CNB2005101109962A CN200510110996A CN100336565C CN 100336565 C CN100336565 C CN 100336565C CN B2005101109962 A CNB2005101109962 A CN B2005101109962A CN 200510110996 A CN200510110996 A CN 200510110996A CN 100336565 C CN100336565 C CN 100336565C
- Authority
- CN
- China
- Prior art keywords
- protein
- plant source
- alcohol
- modulus
- support
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Prostheses (AREA)
- Materials For Medical Uses (AREA)
Abstract
The present invention relates to a plant source protein three-dimensional scaffold material in the biomedical engineering field, which comprises the following components by weight: 25.4 to 82.5% of plant source alcohol-soluble protein, 2.9 to 35.1% of fatty acid and 6.5 to 68.0% of pore-forming agent. After the fatty acid and the plant source alcohol-soluble protein are proportionally mixed, the pore-forming agent is added in the mixture, forming is done by die-pressing, then consolidation forming is done, and freeze drying is done to obtain the three-dimensional scaffold with high mechanical strength and porosity after the pore-forming agent is removed by water bath leaching. The plant source protein three-dimensional scaffold prepared by the material of the present invention has maximum compressive strength at 151.3MPa and modulus at 937Mpa, maximum bending strength at 45.1MPa and modulus at 876Mpa, tensile strength at 11.4Mpa and modulus at 1240MPa, and highest porosity at 82.5%.
Description
Technical field
What the present invention relates to is the bio-medical material in a kind of biomedical engineering technology field, is specifically related to a kind of plant source protein three-dimensional stent material.
Background technology
Tissue engineering bracket material is one of emphasis of biomedical sector Tissue Engineering Study.Biomaterial is the three-dimensional rack that the seed cell formative tissue is depended on for existence and depended on before, it can be with cell fixation in certain position, for physiological activities such as its growth, breeding, metabolism and extracellular matrix secretion provide the place, and guiding regenerating tissues basic configuration.Timbering material as organizational project should possess following characteristics: 1. good biocompatibility and histocompatibility: be beneficial to cell adhesion and propagation, and no cytotoxicity, non-immunogenicity, reaction does not cause inflammation; 2. biodegradability: can degrade fully, product is nontoxic, and degradation rate forms the speed basically identical with tissue; 3. have plasticity and certain mechanical strength, can keep specific size and shape, bootable tissue regeneration; 4. certain porosity and suitably big or small aperture, cell can be evenly distributed on material surface and inside; 5. nontoxic, have no adverse reaction, stable in properties is easily stored, easily sterilization etc.
The mechanical property of biomaterial is an organizational project, particularly one of key factor in the hard tissue engineering.At present, natural biologic material has been subjected to extensive studies, comprises chitin, fibrin, collagen protein, chitosan etc.These material common features are exactly degradable, good biocompatibility, but mechanical strength deficiency, thus limited their development.Zein is the main storage protein of corn, accounts for proteic 45%-50% in the corn.Zein can be processed into resin, has good thermoplastic, tenacity, hydrophobicity, degradability and antibiotic property etc.Zein has been studied and has been used for adhesive of medical, biodegradable plastic, chewing gum, the packaging for foodstuff of edible moisture-resisting, acid-sensitive sense drug delivery system, microsphere, the microencapsulation insecticide, oral drugs odor mask, many aspects such as biological experiment band and wound dressing.Studies show that zein has good biodegradability and biocompatibility, is a kind of new type natural polymer that can be applicable to organizational project.
Find through literature search prior art, the Chinese patent publication number is the patent of CN1555892A, this patent is mixed Phytogenous alcohol-soluble protein with porogen such as Sal, natural molding under the condition of uniform temperature and humidity, or remove the porogen postlyophilization and make through compression molding, decocting in water.Although said method can obtain to have the support of certain mechanical strength and hole characteristic, its mechanical property is relatively poor, and modulus of compressibility is tens MPa only, and material fragility is big, can't obtain tensile property and bending property.Therefore, the application of plant source protein three-dimensional support in organizational project of method for preparing is restricted, and is not suitable for bearing position, particularly the needs of bone tissue engineer.
Summary of the invention
The present invention provides a kind of plant source protein three-dimensional stent material in order to solve the above-mentioned problems in the prior art.Make it have higher mechanical strength and good hole characteristic, this plant source protein three-dimensional stent material contains one or more fatty acids, and its compressive strength, hot strength and three-point bending strength and corresponding modulus are in the mechanical strength scope of normal human's bone.
The present invention is achieved through the following technical solutions, and each component of the present invention and percentage by weight thereof are: Phytogenous alcohol-soluble protein 25.4%-82.5%, fatty acid 2.9%-35.1%, porogen 6.5%-68.0%.After fatty acid and Phytogenous alcohol-soluble protein are mixed in proportion, add porogen again, promptly obtain material of the present invention, through compression molding, reinforce molding then, adopt the water-bath leaching to remove the porogen postlyophilization and make three-dimensional rack with high mechanical strength and porosity.
Described Phytogenous alcohol-soluble protein is the protein,alcohol-soluble of rice, Semen Tritici aestivi, Fructus Hordei Vulgaris, naked barley, Herba bromi japonici or corn.Recommend to use zein.
Described fatty acid comprises satisfied fatty acid such as stearic acid, Palmic acid, and unsaturated fatty acid such as oleic acid, linoleic acid.
Described porogen, any in mannitol, sorbitol, glucose, lactose and the maltose.
The present invention adds the performance that fatty acid improves timbering material, fatty acid is a kind of good plasticiser, they can be penetrated between the chain that the Phytogenous alcohol-soluble protein macromole separates, under higher humidity and temperature conditions by reducing the glass transition temperature of alcohol soluble protein molecule.On the one hand, they reduce the interchain frictional force of plant source protein molecule, thereby make the motion between macromole be easy to take place, and make the intermolecular interaction of alcohol soluble protein strengthen; On the other hand, fatty acid (plasticiser) and the violent interaction of the intermolecular generation of Phytogenous alcohol-soluble protein under higher humidity and temperature conditions.Therefore, after removing porogen, formed porous support with excellent mechanical performances.The raw material sources of material therefor of the present invention are extensive, cheap, technological requirement is low, are suitable as bio-medical material.
The present invention add the timbering material behind the fatty acid mechanical property be improved significantly, comparing with matched group (not adding fatty acid) greatly increases.Greatest compressive strength and modulus with the plant source protein three-dimensional support of the present invention preparation reach 151.3MPa and 937MPa, maximum deflection intensity and modulus reach 45.1MPa and and 876MPa; Hot strength and modulus are respectively 11.4MPa and 1240MPa.Porosity is up to 82.5%.The greatest compressive strength and the modulus of not adding the support of fatty acid are 46.6MPa and 1021MPa, but material fragility is big, do not obtain the result of its corresponding tensile property and bending property.
The specific embodiment
Embodiment one
Each component of plant source protein three-dimensional stent material of the present invention and percentage by weight thereof are: Phytogenous alcohol-soluble protein 82.5%, oleic acid 8.3%, mannitol 9.2%.Described Phytogenous alcohol-soluble protein is a kind of protein,alcohol-soluble that derives from corn.With the said components mixing, compression molding was put in the sample for preparing relative humidity 95-100%, 37-40 ℃ of condition compacted under 21-28 days then.Behind rack forming, support is put in the 50-55 ℃ of water-bath, make to cause mannitol and be dissolved in the water and from support, remove, changed a water, 72 hours leaching time every two hours.Can make gliadin porous stent in 12 hours through vacuum lyophilization again.The sample for preparing adopts Zwick material universal testing machine to carry out Mechanics Performance Testing and adopts gas discharge method to carry out the porosity test.The compressive strength of support and modulus reach 83.3MPa and 877MPa respectively; Bending strength and modulus reach 19.7MPa and 529MPa respectively; Hot strength and modulus are respectively 5.2MPa and 468MPa.The porosity of support reaches 28.0%.
Embodiment two
Each component of plant source protein three-dimensional stent material of the present invention and percentage by weight thereof are: Phytogenous alcohol-soluble protein 65.5%, oleic acid 6.5%, sorbitol 28.0%.Described Phytogenous alcohol-soluble protein is a kind of avenaceous protein,alcohol-soluble that derives from.With the said components mixing, compression molding was put in the sample for preparing relative humidity 95-100%, 37-40 ℃ of condition compacted under 21-28 days then.Behind rack forming, support is put in the 50-55 ℃ of water-bath, make to cause sorbitol and be dissolved in the water and from support, remove, changed a water, 60 hours leaching time every two hours.Can make gliadin porous stent in 10 hours through vacuum lyophilization again.The sample for preparing adopts Zwick material universal testing machine to carry out Mechanics Performance Testing and adopts gas discharge method to carry out the porosity test.The compressive strength of support and modulus reach 15.3MPa and 339MPa respectively; Bending strength and modulus reach 6.2MPa and 308MPa respectively; Hot strength and modulus are respectively 1.6MPa and 378MPa.The porosity of support reaches 53.5%.
Embodiment three
Each component of plant source protein three-dimensional stent material of the present invention and percentage by weight thereof are: Phytogenous alcohol-soluble protein 29.1%, linoleic acid 2.9%, lactose 68.0%.Described Phytogenous alcohol-soluble protein is a kind of protein,alcohol-soluble that derives from Fructus Hordei Vulgaris.With the said components mixing, compression molding was put in the sample for preparing relative humidity 95-100%, 37-40 ℃ of condition compacted under 21-28 days then.Behind rack forming, support is put in the 50-55 ℃ of water-bath, make to cause lactose and be dissolved in the water and from support, remove, changed a water, 48 hours leaching time every two hours.Can make gliadin porous stent in 8 hours through vacuum lyophilization again.The sample for preparing adopts Zwick material universal testing machine to carry out Mechanics Performance Testing and adopts gas discharge method to carry out the porosity test.The compressive strength of support and modulus reach 3.9MPa and 27MPa respectively; Bending strength and modulus reach 1.3MPa and 19MPa respectively; Hot strength and modulus are respectively 0.5MPa and 9MPa.The porosity of support reaches 82.5%.
Embodiment four
Each component of plant source protein three-dimensional stent material of the present invention and percentage by weight thereof are: Phytogenous alcohol-soluble protein 76.2%, linoleic acid 15.3%, glucose 8.5%.Described Phytogenous alcohol-soluble protein is a kind of protein,alcohol-soluble that derives from rice.With the said components mixing, compression molding was put in the sample for preparing relative humidity 95-100%, 37-40 ℃ of condition compacted under 21-28 days then.Behind rack forming, support is put in the 50-55 ℃ of water-bath, make to cause glucose and be dissolved in the water and from support, remove, changed a water, 72 hours leaching time every two hours.Can make gliadin porous stent in 12 hours through vacuum lyophilization again.The sample for preparing adopts Zwick material universal testing machine to carry out Mechanics Performance Testing and adopts gas discharge method to carry out the porosity test.The compressive strength of support and modulus reach 121.3MPa and 937MPa respectively; Bending strength and modulus reach 35.2MPa and 876MPa respectively; Hot strength and modulus are respectively 7.4MPa and 1240MPa.The porosity of support reaches 25.0%.
Embodiment five
Each component of plant source protein three-dimensional stent material of the present invention and percentage by weight thereof are: Phytogenous alcohol-soluble protein 61.4%, oleic acid 12.3%, mannitol 26.3%.Described Phytogenous alcohol-soluble protein is a kind of protein,alcohol-soluble that derives from corn.With the said components mixing, compression molding was put in the sample for preparing relative humidity 95-100%, 37-40 ℃ of condition compacted under 21-28 days then.Behind rack forming, support is put in the 50-55 ℃ of water-bath, make to cause mannitol and be dissolved in the water and from support, remove, changed a water, 60 hours leaching time every two hours.Can make gliadin porous stent in 10 hours through vacuum lyophilization again.The sample for preparing adopts Zwick material universal testing machine to carry out Mechanics Performance Testing and adopts gas discharge method to carry out the porosity test.The compressive strength of support and modulus reach 51.8MPa and 587MPa respectively; Bending strength and modulus reach 18.2MPa and 521MPa respectively; Hot strength and modulus are respectively 4.1MPa and 762MPa.The porosity of support reaches 41.5%.
Embodiment six
Each component of plant source protein three-dimensional stent material of the present invention and percentage by weight thereof are: Phytogenous alcohol-soluble protein 28.7%, Palmic acid 4.3%, mannitol 67.0%.Described Phytogenous alcohol-soluble protein is a kind of avenaceous protein,alcohol-soluble that derives from.With the said components mixing, compression molding was put in the sample for preparing relative humidity 95-100%, 37-40 ℃ of condition compacted under 21-28 days then.Behind rack forming, support is put in the 50-55 ℃ of water-bath, make to cause mannitol and be dissolved in the water and from support, remove, changed a water, 48 hours leaching time every two hours.Can make gliadin porous stent in 8 hours through vacuum lyophilization again.The sample for preparing adopts Zwick material universal testing machine to carry out Mechanics Performance Testing and adopts gas discharge method to carry out the porosity test.The compressive strength of support and modulus reach 4.9MPa and 19MPa respectively; Bending strength and modulus reach 1.6MPa and 17MPa respectively; Hot strength and modulus are respectively 0.5MPa and 14MPa.The porosity of support reaches 81.5%.
Embodiment seven
Each component of plant source protein three-dimensional stent material of the present invention and percentage by weight thereof are: Phytogenous alcohol-soluble protein 58.4%, stearic acid 35.1%, maltose 6.5%.Described Phytogenous alcohol-soluble protein is a kind of protein,alcohol-soluble that derives from Semen Tritici aestivi.With the said components mixing, compression molding was put in the sample for preparing relative humidity 95-100%, 37-40 ℃ of condition compacted under 21-28 days then.Behind rack forming, support is put in the 50-55 ℃ of water-bath, make to cause maltose and be dissolved in the water and from support, remove, changed a water, 72 hours leaching time every two hours.Can make gliadin porous stent in 12 hours through vacuum lyophilization again.The sample for preparing adopts Zwick material universal testing machine to carry out Mechanics Performance Testing and adopts gas discharge method to carry out the porosity test.The compressive strength of support and modulus reach 12.7MPa and 249MPa respectively; Bending strength and modulus reach 5.1MPa and 196MPa respectively; Hot strength and modulus are respectively 1.4MPa and 208MPa.The porosity of support reaches 18.5%.
Embodiment eight
Each component of plant source protein three-dimensional stent material of the present invention and percentage by weight thereof are: Phytogenous alcohol-soluble protein 49.3%, oleic acid 29.6%, sorbitol 21.1%.Described Phytogenous alcohol-soluble protein is a kind of protein,alcohol-soluble that derives from naked barley.With the said components mixing, compression molding was put in the sample for preparing relative humidity 95-100%, 37-40 ℃ of condition compacted under 21-28 days then.Behind rack forming, support is put in the 50-55 ℃ of water-bath, make to cause sorbitol and be dissolved in the water and from support, remove, changed a water, 72 hours leaching time every two hours.Can make gliadin porous stent in 10 hours through vacuum lyophilization again.The sample for preparing adopts Zwick material universal testing machine to carry out Mechanics Performance Testing and adopts gas discharge method to carry out the porosity test.The compressive strength of support and modulus reach 15.3MPa and 223MPa respectively; Bending strength and modulus reach 4.5MPa and 131MPa respectively; Hot strength and modulus are respectively 1.7MPa and 213MPa.The porosity of support reaches 49.5%.
Embodiment nine
Each component of plant source protein three-dimensional stent material of the present invention and percentage by weight thereof are: Phytogenous alcohol-soluble protein 25.4%, Palmic acid 15.3%, mannitol 59.3%.Described Phytogenous alcohol-soluble protein is a kind of protein,alcohol-soluble that derives from corn.With the said components mixing, compression molding was put in the sample for preparing relative humidity 95-100%, 37-40 ℃ of condition compacted under 21-28 days then.Behind rack forming, support is put in the 50-55 ℃ of water-bath, make to cause mannitol and be dissolved in the water and from support, remove, changed a water, 48 hours leaching time every two hours.Can make gliadin porous stent in 8 hours through vacuum lyophilization again.The sample for preparing adopts Zwick material universal testing machine to carry out Mechanics Performance Testing and adopts gas discharge method to carry out the porosity test.The compressive strength of support and modulus reach 8.9MPa and 159MPa respectively; Bending strength and modulus reach 3.6MPa and 97MPa respectively; Hot strength and modulus are respectively 1.5MPa and 114MPa.The porosity of support reaches 61.5%.
Claims (3)
1. a plant source protein three-dimensional stent material is characterized in that, each component and percentage by weight thereof are: Phytogenous alcohol-soluble protein 25.4%-82.5%, fatty acid 2.9%-35.1%, porogen 6.5%-68.0%;
Described fatty acid, any in stearic acid, Palmic acid, oleic acid and the linoleic acid;
Described porogen, any in mannitol, sorbitol, glucose, lactose and the maltose.
2. plant source protein three-dimensional stent material according to claim 1 is characterized in that, described Phytogenous alcohol-soluble protein is the protein,alcohol-soluble of rice, Semen Tritici aestivi, Fructus Hordei Vulgaris, naked barley, Herba bromi japonici or corn.
3. according to claim 1 or 2 described plant source protein three-dimensional stent materials, it is characterized in that described Phytogenous alcohol-soluble protein is zein.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005101109962A CN100336565C (en) | 2005-12-01 | 2005-12-01 | Plant source protein three-dimensional stent material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005101109962A CN100336565C (en) | 2005-12-01 | 2005-12-01 | Plant source protein three-dimensional stent material |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1775307A CN1775307A (en) | 2006-05-24 |
| CN100336565C true CN100336565C (en) | 2007-09-12 |
Family
ID=36765104
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2005101109962A Expired - Fee Related CN100336565C (en) | 2005-12-01 | 2005-12-01 | Plant source protein three-dimensional stent material |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100336565C (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1456361A (en) * | 2002-07-05 | 2003-11-19 | 马东兰 | Biomedical materials and preparing method thereof |
| CN1555892A (en) * | 2004-01-08 | 2004-12-22 | 上海交通大学 | Preparation method of plant origion alcohrl soluble protein three dimentional support |
-
2005
- 2005-12-01 CN CNB2005101109962A patent/CN100336565C/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1456361A (en) * | 2002-07-05 | 2003-11-19 | 马东兰 | Biomedical materials and preparing method thereof |
| CN1555892A (en) * | 2004-01-08 | 2004-12-22 | 上海交通大学 | Preparation method of plant origion alcohrl soluble protein three dimentional support |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1775307A (en) | 2006-05-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108864494B (en) | Dynamic cross-linked double-network hydrogel and preparation method and application thereof | |
| Janarthanan et al. | 3D printable and injectable lactoferrin-loaded carboxymethyl cellulose-glycol chitosan hydrogels for tissue engineering applications | |
| Fernandes et al. | Bionanocomposites from lignocellulosic resources: Properties, applications and future trends for their use in the biomedical field | |
| Pighinelli et al. | Chitosan–hydroxyapatite composites | |
| Zhang et al. | Synthesis and characterization of hyaluronic acid/human-like collagen hydrogels | |
| Indurkar et al. | Plant-based biomaterials in tissue engineering | |
| Ifkovits et al. | Biodegradable and radically polymerized elastomers with enhanced processing capabilities | |
| Möller et al. | Dextran and hyaluronan methacrylate based hydrogels as matrices for soft tissue reconstruction | |
| Siritientong et al. | Characteristics of carboxymethyl cellulose/sericin hydrogels and the influence of molecular weight of carboxymethyl cellulose | |
| Varshney et al. | Freeze–thaw-induced physically cross-linked superabsorbent polyvinyl alcohol/soy protein isolate hydrogels for skin wound dressing: In vitro and in vivo characterization | |
| EP3620186A1 (en) | Biomaterial devices and topical compositions for guided tissue regeneration | |
| Kumar et al. | A comprehensive review of various biopolymer composites and their applications: from biocompatibility to self-healing | |
| Emre Oz et al. | A review of functionalised bacterial cellulose for targeted biomedical fields | |
| Bierhalz et al. | Composite membranes of alginate and chitosan reinforced with cotton or linen fibers incorporating epidermal growth factor | |
| Gou et al. | Advances and prospects of Bletilla striata polysaccharide as promising multifunctional biomedical materials | |
| Wang et al. | A high strength semi-degradable polysaccharide-based hybrid hydrogel for promoting cell adhesion and proliferation | |
| Nath et al. | Recent advances in production of sustainable and biodegradable polymers from agro-food waste: Applications in tissue engineering and regenerative medicines | |
| Zhuang et al. | Honeycomb structural composite polymer network of gelatin and functional cellulose ester for controlled release of omeprazole | |
| Badhe et al. | Cellulosic materials as bioinks for 3D printing applications | |
| CN100336565C (en) | Plant source protein three-dimensional stent material | |
| KR101443673B1 (en) | Method for manufacturing hyaluronic acid derivatives sponge and hyaluronic acid derivatives manufactured thereby | |
| De Carvalho et al. | Biomedical applications for thermoplastic starch | |
| CN100486648C (en) | Method for preparing plant source protein three-dimensional steut with improved property | |
| KR20160127672A (en) | Adhesion barrier containing natural-polymer with biocompatibility and DNA fragment mixture, and process for producing the same | |
| CN111228562B (en) | Starch hemostatic sponge and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20070912 Termination date: 20161201 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |