CN100335141C - Method for preparing bionic multi-layered structure cartilage implant material - Google Patents
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- CN100335141C CN100335141C CNB200510012257XA CN200510012257A CN100335141C CN 100335141 C CN100335141 C CN 100335141C CN B200510012257X A CNB200510012257X A CN B200510012257XA CN 200510012257 A CN200510012257 A CN 200510012257A CN 100335141 C CN100335141 C CN 100335141C
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Abstract
The present invention provides a method for preparing a bionic multi-layered structure cartilage implant material, which belongs to the technical field of a biological medical material or a biological composite material. The process comprises the preparation of a lubricating layer, a biological active layer and hydrogel with multi-layered structure. The composite solution of PVA / a bioactive component is filled in cylindrical or rectangular moulds, then the PVA solution is filled in, and pressure is applied to compact. The PVA solution for a lubricating layer is filled in, and the compaction is carried out by pressurizing. The moulds are arranged in a refrigerator of which the temperature is from-10 to 40 DEG C, repeated refrigeration and fusion are done for 2-8 times, and a bionic multi-layered structure cartilage implant material is obtained. The present invention has the advantages that the surface layer of the obtained cartilage implant material is a wear-resistance lubricating layer, the middle layer is a stressing layer, the bottom layer is a cartilage bioactive connection layer, the surface friction coefficient is less than 0.1 on the occasion of milling without water, the elastic modulus is greater than 15MPa, the natural cartilage performance is obtained, and the excellent surface lubricity and the bioactivity connectivity are imparted while high requirements for the biomechanics performance of the cartilage implant material are met.
Description
Technical field
The invention belongs to bio-medical material or Biocomposite material technical field, a kind of preparation method of bionic multi-layered structure cartilage implant material particularly is provided.
Background technology
In organism, cartilaginous tissue bears the load of bone conduction, can absorb concussion, the effect of the friction and wear when playing buffering extrusion stress and shear stress and reducing the articular surface motion.Cartilage is made up of chondrocyte and substrate, and normal ripe chondrocyte metabolism is slow, and the Growth and Differentiation ability is low.Damage or pathological changes be in case take place in cartilaginous tissue, himself repair and self-healing ability extremely limited, must carry out the reparation and the transplanting of articular cartilage.The cartilaginous tissue disease is the common phenomenon of clinical Orthopeadic Surgery, and annual nearly 100,000 of China is the arthrosis patient who is caused by cartilage lesion according to statistics.The treatment of articular cartilage is mainly adopted from body or allosome cartilage transplantation and articular prosthesis displacement clinically at present, but there are problems such as limited, potential virus spread of donor source and immunologic rejection in the allosome cartilage transplantation, therefore must research and develop artificial cartilage material and implant with excellent biomechanical property and alternative cartilage function and satisfy the demand that articular cartilage is treated.
The more artificial cartilage material of research mainly contains metal-ultra-high molecular weight polyethylene to mill articular prosthesis, silicone rubber, polyurethane joint cushion material, polyvinyl alcohol hydrogel etc. at present.Though these cartilage prostheses can play the effect that substitutes cartilage to a certain extent, also there are loosening, deficiencies such as boundary lubrication is poor, shortage biological activity easy to wear, especially in composition and structural unicity, be difficult to the real cartilage function of realizing replacing.For example, methods such as employing crosslinking with radiation and freezing-fusion are crosslinked can obtain the polyvinyl alcohol hydrogel elastomer of high-crystallinity, have excellent intensity, elasticity and extension performance, and excellent biological compatibility, high-moisture, many micropore tissues and water permeability, its physicochemical property and biology performance more near biological tissue, are a kind of comparatively ideal cartilage substitution material than other synthetic material.But the PVA hydrogel surface is smooth, there is not biological activity, during as cartilage prosthese implantation joint position and the binding ability of bone substrate poor, influenced the fixing and repair function of cartilage, we adopt PVA and compound this problem (relevant technologies has obtained the national inventing patent mandate) that solves of biological activity hydroxyapatite, but bring the problem that causes the PVA lubricity to descend simultaneously again; And the method for existing raising cartilage prosthetic surface lubricity may make its biomechanical property descend again.How to give cartilage implant material high biomechanical property, make it have excellent surface lubrication and biological activity connectivity simultaneously, this is both at home and abroad all less than a difficult problem that solves.
As everyone knows, variation can be divided into shallow top layer, middle level, deep layer and calcification layer to sophisticated articular cartilage according to 26S Proteasome Structure and Function.Wherein the cell on shallow top layer is fusiformis, and major axis is parallel to articular surface, and collagen fiber are tangentially arranged, and mainly bears shear load and lubrication; The chondrocyte of middle level and deep layer gradually ovalize, circle, until cylindricality, the thick and vertical direction of collagen fiber is arranged, and mainly bears nip load; The calcification substrate of calcification layer and the part longitudinal fiber of deep layer play a part articular cartilage face is securely fixed on the subchondral bone matter.The 26S Proteasome Structure and Function of its different levels has guaranteed the biomechanical property and the physiological function of cartilage.For this reason, the present invention is according to the structure of natural cartilage, and having proposed a kind of top layer is wear-resisting lubricating layer, and the intermediate layer is a stress layer, and bottom is the preparation method of the novel bionic multi-layered structure cartilage implant material of bone bioactivity articulamentum.
Summary of the invention
The object of the present invention is to provide a kind of preparation method of bionic multi-layered structure cartilage implant material, can obtain a kind of top layer by this method is wear-resisting lubricating layer, the intermediate layer is a stress layer, bottom is the bionic multi-layered structure composite aquogel artificial cartilage embedded material of bone bioactivity articulamentum, fundamentally solved the problem that existing hydrogel soft bone material is difficult to satisfy simultaneously lubricity, biological activity and biomechanical property, made the artificial cartilage material more approaching with natural cartilage on 26S Proteasome Structure and Function.
Preparation technology of the present invention is as follows:
In a, the redistilled water that the refining back of analytical pure polyvinyl alcohol (PVA) is dissolved in, be formulated as weight percent concentration and be homogeneous aqueous solution's (solution I) of 15～30% in 85～95 ℃ of waters bath with thermostatic control or 110～150MPa pressure cooker.
B, lubricating layer preparation: in the PVA aqueous solution, add polyvinylpyrrolidone (PVP), chitosan, hyaluronic acid or hyaluronate sodium (HA) respectively, wherein polyvinylpyrrolidone and chitosan addition are 5～40% of PVA weight, and chitosan should dissolve in 0.1～20% dilute acid soln after 2～48 hours in adding PVA solution; HA is dissolved in earlier and is formulated as homogeneous solution in the normal saline, adds in the PVA aqueous solution again, and the concentration of HA in PVA solution is 0.1～3% percentage by weight.Above-mentioned mixed solution 85～95 ℃ stir down fully after, left standstill 12～48 hours or vacuum exhaust 2～8 hours, get rid of (solution II) fully to bubble.
C; bioactive layer preparation: adopt hydroxyapatite respectively; coral hydroxyapatite; bata-tricalcium phosphate; the granule of bioactivity glass and homogeneous allogenic bone or block and PVA aqueous solution are compound; can adopt one of following method (1) respectively with hydroxyapatite; coral hydroxyapatite; bata-tricalcium phosphate; bioactivity glass and homogeneous allogenic bone etc. are ground to below the 30 μ m; be added in 85～95 ℃ of PVA aqueous solutions; the content of bioactive ingredients is 5～20% of PVA weight; and handle more than 1～5 hour with the ultra-sonic dispersion instrument, obtain even aaerosol solution III.(2) under vigorous stirring be that 2%～20% alcohol calcium solution adds in the PVA solution with weight percent concentration; By the Ca/P ratio is 1.0～3.0 to be mixed with concentration H
3PO
4Ethanol solution after vibration stirs, slowly is added drop-wise in the above-mentioned PVA solution, fully obtains PVA/ nanometer hydroxyapatite or PVA/ nano tricalcium phosphate composite solution (solution III) behind the stirring reaction.(3) respectively with the cellular hydroxyapatite, coral hydroxyapatite, bata-tricalcium phosphate, block such as bioactivity glass and homogeneous allogenic bone is cut into square or cylindrical shape, place the mould of band piston, inject PVA solution and it is full of porous material fully, obtain liquid-solid compound mixed solution (solution III) with piston action.
D, the preparation of multiple structure hydrogel: solution III is infused in the cylindric or orthogonal mould, and thickness is 0.1～10mm, applies the pressure compacting of 0.1～10MPa; The solution I of reinjecting, thickness 1～20mm; The compacting of exerting pressure; Inject solution II, thickness is 0.05～5mm, the pressurization compacting.
E, above-mentioned mould inserted in-10～-40 ℃ the refrigerator, freezing 3～24 hours,,, promptly obtain a kind of bionic multi-layered structure cartilage implant material through freezing repeatedly-fusion 2～8 times then in 15～40 ℃ of fusions 2～10 hours.
The present invention compared with prior art has following advantage and beneficial effect::
Adopt preparation method provided by the invention, can obtain a kind of top layer is wear-resisting lubricating layer, the intermediate layer is a stress layer, bottom is the novel bionic multi-layered structure cartilage implant material of bone bioactivity articulamentum, similar to the 26S Proteasome Structure and Function of natural cartilage, the 26S Proteasome Structure and Function of its different levels has guaranteed the biomechanical property and the physiological function of cartilage, adopt cartilage implant material that this method obtains skin-friction coefficient can be less than 0.1 during to mill anhydrous, elastic modelling quantity can meet or exceed the performance of natural cartilage greater than 15MPa; Implant animal cartilage after 8 weeks, having can be observed osseous tissue grows in the cartilage implant, be that cartilage material has formed firm bone with the bone substrate and is connected, realized when satisfying the high biomechanical property of cartilage implant material and requiring, giving its excellent surface lubrication and biological activity connectivity.Adopt the resulting multilayer material of this method in addition, combine closely between layer and the layer, before bulk material destroys, can obviously not demarcate or peel off between each layer, have good structure and stability.
The specific embodiment
Embodiment 1
A, with the refining back of analytical pure polyvinyl alcohol (PVA) in 90 ℃ of waters bath with thermostatic control, be formulated as weight percent concentration and be homogeneous aqueous solution's (solution I) of 15%.
B, lubricating layer preparation: add polyvinylpyrrolidone (PVP) in the PVA aqueous solution, addition is 10% of a PVA weight, and mixed solution left standstill 24 hours after 90 ℃ of abundant down stirrings, obtained solution II after bubble is got rid of fully.
C, bioactive layer preparation: coral hydroxyapatite is ground to below the 30 μ m, is added in 90 ℃ of PVA aqueous solutions, the content of coral hydroxyapatite is 10% of PVA weight, and handles 3 hours with the ultra-sonic dispersion instrument, obtains even aaerosol solution III.
D, the preparation of multiple structure hydrogel: solution III is infused in the cylindric or quadrate mould, and thickness is 1mm, applies the pressure compacting of 0.3MPa; The solution I of reinjecting, thickness 2mm; The compacting of exerting pressure; Inject solution II, thickness is 0.5mm, the pressurization compacting.
E, above-mentioned mould inserted in-20 ℃ the refrigerator, freezing 6 hours,,, promptly obtain a kind of bionic multi-layered structure cartilage implant material through freezing repeatedly-fusion 4 times then in 25 ℃ of fusions 8 hours.
Embodiment 2
A, with the refining back of analytical pure polyvinyl alcohol (PVA) in 95 ℃ of waters bath with thermostatic control, be formulated as weight percent concentration and be homogeneous aqueous solution's (solution I) of 30%.
B, lubricating layer prepare: chitosan is dissolved 24 hours in 0.5% acetic acid,diluted solution after, add in the PVA solution, the chitosan addition is 8% of a PVA weight; After 95 ℃ of abundant down stirrings, vacuum exhaust 6 hours is got rid of fully to bubble, obtains solution II with mixed solution.
C, bioactive layer preparation: bioactivity glass is ground to below the 30 μ m, is added in 95 ℃ of PVA aqueous solutions, its content is 15% of PVA weight, handles more than 5 hours with the ultra-sonic dispersion instrument, obtains even aaerosol solution III.
D, the preparation of multiple structure hydrogel: solution III is infused in the columned mould, and thickness is 3mm, applies the pressure compacting of 3MPa; The solution I of reinjecting, thickness 5mm; The compacting of exerting pressure; Inject solution II, thickness is 1mm, the pressurization compacting.
E, above-mentioned mould inserted in-30 ℃ the refrigerator, freezing 4 hours,,, promptly obtain a kind of bionic multi-layered structure cartilage implant material through freezing repeatedly-fusion 6 times then in 30 ℃ of fusions 10 hours.
Embodiment 3
In a, the redistilled water that the refining back of analytical pure polyvinyl alcohol (PVA) is dissolved in, be formulated as weight percent concentration and be homogeneous aqueous solution's (solution I) of 20% in the 125MPa pressure cooker.
The preparation of b, lubricating layer: hyaluronic acid (HA) is dissolved in is formulated as homogeneous solution in the normal saline, add in the PVA aqueous solution, the concentration of HA in PVA solution is 0.15% percentage by weight.Above-mentioned mixed solution left standstill 48 hours after 95 ℃ of abundant down stirrings, obtained solution II after bubble is got rid of fully.
C, bioactive layer preparation: under vigorous stirring be that 5% alcohol calcium solution adds in the PVA solution with weight percent concentration; By the Ca/P ratio is 2.0 to be mixed with concentration H
3PO
4Ethanol solution after vibration stirs, slowly is added drop-wise in the above-mentioned PVA solution, fully obtains PVA/ nano tricalcium phosphate composite solution (solution III) behind the stirring reaction.
D, the preparation of multiple structure hydrogel: solution III is infused in the rectangular mould, and thickness is 5mm, applies the pressure compacting of 5MPa; The solution I of reinjecting, thickness 8mm; The compacting of exerting pressure; Inject solution II, thickness is 2mm, the pressurization compacting.
E, above-mentioned mould inserted in-20 ℃ the refrigerator, freezing 10 hours,,, promptly obtain a kind of bionic multi-layered structure cartilage implant material through freezing repeatedly-fusion 8 times then in 25 ℃ of fusions 8 hours.
Embodiment 4
In a, the redistilled water that the refining back of analytical pure polyvinyl alcohol (PVA) is dissolved in, be formulated as weight percent concentration and be homogeneous aqueous solution's (solution I) of 25% in the 115MPa pressure cooker.
The preparation of b, lubricating layer: hyaluronate sodium (HA) is dissolved in is formulated as homogeneous solution in the normal saline, add in the PVA aqueous solution again, the concentration of HA in PVA solution is 2.5% percentage by weight.Above-mentioned mixed solution 90 ℃ stir down fully after, vacuum exhaust 8 hours is got rid of fully to bubble and to be obtained solution II.
C, bioactive layer preparation: cellular homogeneous allogenic bone block is cut into square, places the mould of band piston, injects PVA solution and with piston action it is full of porous material fully, obtains liquid-solid compound mixed solution (solution III).
D, the preparation of multiple structure hydrogel: solution III is injected rectangular mould, and thickness is 8mm, applies the pressure compacting of 9MPa; The solution I of reinjecting, thickness 15mm; The compacting of exerting pressure; Inject solution II, thickness is 5mm, the pressurization compacting.
E, above-mentioned mould inserted in-35 ℃ the refrigerator, freezing 20 hours,,, promptly obtain a kind of bionic multi-layered structure cartilage implant material through freezing repeatedly-fusion 6 times then in 35 ℃ of fusions 8 hours.
Embodiment 5
A, with the refining back of analytical pure polyvinyl alcohol (PVA) in 95 ℃ of waters bath with thermostatic control, be formulated as weight percent concentration and be homogeneous aqueous solution's (solution I) of 25%.
B, lubricating layer preparation: add polyvinylpyrrolidone (PVP) in the PVA aqueous solution, addition is 20% of a PVA weight, and mixed solution is after 95 ℃ of abundant down stirrings, and vacuum suction 6 hours obtains solution II after bubble is got rid of fully.
C, bioactive layer preparation: bioactive layer preparation: under vigorous stirring be that 5% alcohol calcium solution adds in the PVA solution with weight percent concentration; By the Ca/P ratio is 1.67 to be mixed with concentration H
3PO
4Ethanol solution after vibration stirs, slowly is added drop-wise in the above-mentioned PVA solution, fully obtains PVA/ nanometer hydroxyapatite composite solution (solution III) behind the stirring reaction.
D, the preparation of multiple structure hydrogel: solution III is injected cylindrical mold, and thickness is 4mm, applies the pressure compacting of 1MPa; The solution I of reinjecting, thickness is 8mm; The compacting of exerting pressure; Inject solution II, thickness is 2mm, the pressurization compacting.
E, above-mentioned mould inserted in-20 ℃ the refrigerator, freezing 6 hours,,, promptly obtain a kind of bionic multi-layered structure cartilage implant material through freezing repeatedly-fusion 4 times then in 30 ℃ of fusions 8 hours.
Claims (4)
1, a kind of preparation method of bionic multi-layered structure cartilage implant material is characterized in that: preparation technology is:
A, lubricating layer preparation: in the redistilled water that the refining back of analytical pure PVAC polyvinylalcohol is dissolved in, be formulated as weight percent concentration and be 15～30% homogeneous aqueous solution in 85～95 ℃ of waters bath with thermostatic control or 110～150MPa pressure cooker; Respectively at the PVA aqueous solution, be called and add polyvinylpyrrolidone PVP, chitosan, hyaluronic acid or hyaluronate sodium HA in the solution I, wherein polyvinylpyrrolidone and chitosan addition are 5～40% of PVA weight, and chitosan should dissolve in 0.1～20% dilute acid soln after 2～48 hours in adding PVA solution; HA is dissolved in earlier and is formulated as homogeneous solution in the normal saline, adds in the PVA aqueous solution again, and the concentration of HA in PVA solution is 0.1～3% percentage by weight; Above-mentioned mixed solution 85～95 ℃ stir down fully after, left standstill 12～48 hours or vacuum exhaust 2～8 hours, get rid of fully to bubble and obtain solution II;
B, bioactive layer preparation: adopt the granule of hydroxyapatite, coral hydroxyapatite, bata-tricalcium phosphate, bioactivity glass and homogeneous allogenic bone or block and PVA aqueous solution compound respectively, obtain PVA/ bioactive ingredients composite solution, be called solution III;
C, the preparation of multiple structure hydrogel: solution III is infused in the cylindric or orthogonal mould, and thickness is 0.1～10mm, applies the pressure compacting of 0.1～10MPa; The solution I of reinjecting, thickness 1～20mm; The compacting of exerting pressure; Inject solution II, thickness is 0.05～5mm, the pressurization compacting;
D, above-mentioned mould inserted in-10～-40 ℃ the refrigerator, freezing 3～24 hours,,, obtain a kind of bionic multi-layered structure cartilage implant material through freezing repeatedly-fusion 2～8 times then in 15～40 ℃ of fusions 2～10 hours.
2, preparation method according to claim 1, it is characterized in that, the preparation method of bioactive layer solution III is: hydroxyapatite, coral hydroxyapatite, bata-tricalcium phosphate, bioactivity glass and homogeneous allogenic bone are ground to below the 30 μ m respectively, be added in 85～95 ℃ of PVA aqueous solutions, the content of bioactive ingredients is 5～20% of PVA weight, and handle more than 1～5 hour with the ultra-sonic dispersion instrument, obtain even aaerosol solution III.
3, preparation method according to claim 1 is characterized in that, the preparation method of bioactive layer solution III is: under vigorous stirring be that 2%～20% alcohol calcium solution adds in the PVA solution with weight percent concentration; By the Ca/P ratio is 1.0～3.0 to be mixed with concentration H
3PO
4Ethanol solution after vibration stirs, slowly is added drop-wise in the above-mentioned PVA solution, fully obtains PVA/ nanometer hydroxyapatite or PVA/ nano tricalcium phosphate composite solution behind the stirring reaction.
4, preparation method according to claim 1, it is characterized in that: the preparation method of bioactive layer solution III is: respectively with the cellular hydroxyapatite, coral hydroxyapatite, bata-tricalcium phosphate, bioactivity glass and homogeneous allogenic bone block are cut into square or cylindrical shape, place the mould of band piston, inject PVA solution and under piston action, it is full of porous material fully, obtain liquid-solid compound mixed solution.
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Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100418587C (en) * | 2006-12-08 | 2008-09-17 | 北京科技大学 | Method for preparing bionic laminar articular cartilage/bone compound implant |
| CN101279109B (en) * | 2008-05-27 | 2011-04-20 | 厦门大学 | Artificial joint medium layer and method of preparing the same |
| CN101554493B (en) * | 2008-07-31 | 2013-01-23 | 华南理工大学 | Method for preparing nano hydroxyapatite/collagen scaffold with directionally arranged particles |
| CN101480501B (en) * | 2009-01-07 | 2012-10-24 | 四川大学 | Substitute material for repairing articular cartilage and preparation method |
| CN102861361B (en) * | 2012-09-18 | 2014-07-02 | 中国科学院宁波材料技术与工程研究所 | High-strength continuous gradient composite scaffold and preparation method thereof |
| CN102973987B (en) * | 2012-12-10 | 2014-03-12 | 宁波保税区安杰脉德医疗器械有限公司 | Medical hydrophilic lubrication coating of water system and preparation method thereof |
| CN103099689B (en) * | 2013-02-19 | 2015-08-26 | 哈尔滨朝阳滨辉实业发展集团有限公司 | The Minimally Invasive Surgery preparation method of spiral nucleus prosthesis and application |
| CN106421889A (en) * | 2016-08-24 | 2017-02-22 | 胡方 | Regenerative medical material and preparation method and application thereof |
| CN115400265B (en) * | 2022-08-05 | 2024-06-21 | 河北医科大学口腔医院 | Bionic gradient stent and preparation method thereof |
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