CL2013002736A1 - Use of annexin a1, regul and igz genes to determine the efficacy of a phenotype vaccine susceptible to infectious salmon anemia (isa) in Atlantic salmon (salmo salar) - Google Patents

Use of annexin a1, regul and igz genes to determine the efficacy of a phenotype vaccine susceptible to infectious salmon anemia (isa) in Atlantic salmon (salmo salar)

Info

Publication number
CL2013002736A1
CL2013002736A1 CL2013002736A CL2013002736A CL2013002736A1 CL 2013002736 A1 CL2013002736 A1 CL 2013002736A1 CL 2013002736 A CL2013002736 A CL 2013002736A CL 2013002736 A CL2013002736 A CL 2013002736A CL 2013002736 A1 CL2013002736 A1 CL 2013002736A1
Authority
CL
Chile
Prior art keywords
sequence
isa
genes
salmon
igz
Prior art date
Application number
CL2013002736A
Other languages
Spanish (es)
Inventor
Soto Ruben Rodrigo Vidal
Robert Mitch Deerenberg
Original Assignee
Univ Santiago Chile
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Santiago Chile filed Critical Univ Santiago Chile
Priority to CL2013002736A priority Critical patent/CL2013002736A1/en
Publication of CL2013002736A1 publication Critical patent/CL2013002736A1/en
Priority to NO20141150A priority patent/NO20141150A1/en
Priority to CA2864728A priority patent/CA2864728A1/en

Links

Classifications

    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish

Landscapes

  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

MÉTODO PARA DETERMINAR LA EFICACIA DE VACUNAS EN FENOTIPOS SUSCEPTIBLES A LA ANEMIA INFECCIOSA DEL SALMON (ISA) EN SALMÓN ATLÁNTICO (SALMO SALAR), QUE COMPRENDE VALORIZAR LA EXPRESIÓN DE LOS GENES ANEXINA, LGZ Y REGUL POR LA TÉCNICA PCR TIEMPO REAL, CUANTIFICANDO EL PERFIL DE EXPRESIÓN DE DICHOS GENES, DONDE SE COMPARA EL PATRÓN DE EXPRESIÓN DE UNA MUESTRA RENAL PARA DESAFÍO POR COHABITACIÓN A ISA, CON EL PATRÓN DE EXPRESIÓN DE DICHOS GENES, EN UNA MUESTRA CONTROL SIN DESAFÍO, Y LA NO PRESENCIA DE UNA DIFERENCIA ESTADÍSTICA SIGNIFICATIVA ANOVA (P <0,001), ES INDICATIVA DE UNA VACUNA EFICAZ PARA FENOTIPO SUSCEPTIBLE A ISA, ES DECIR, CON UN CARÁCTER QUE PRESENTA COMO CARACTERÍSTICA, UNA MORTALIDAD SUPERIOR A 50% PARA EL VIRUS ISA Y UNA HEREDABILIDAD DE DICHO CARÁCTER MENOR O IGUAL A 0,34, Y DONDE LA TÉCNICA PCR EN TIEMPO REAL SE REALIZA CON PARTIDORES SELECCIONADOS DEL GRUPO CONSISTENTE DE: ANEXINA AL DE SECUENCIA SENTIDO CTCCAGGAAATTGAACACCGCGA Y SECUENCIA ANTI-SENTIDO AAGGCTGCGATGAAGGACATGGT, IGZ SECUENCIA SENTIDO AGCACCAGGGACAACCACCAT Y SECUENCIA ANTISENTIDO TTCACACTCGGTGGGTTAGAGTC Y REGUL F DE SECUENCIA SENTIDO GACTCCTAACCTCCAATGCTTCGAC Y SECUENCIA ANTISENTIDO CGAATCTCTCTCCATCAGCCCATA.        METHOD FOR DETERMINING THE EFFECTIVENESS OF VACCINES IN PHENOTYPES SUSCEPTIBLE TO THE INFECTIOUS ANEMIA OF THE SALMON (ISA) IN ATLANTIC SALMON (SALMO SALAR), WHICH INCLUDES VALUING THE EXPRESSION OF THE GENES ANNEXINES, LGZ AND REGULATES BY THE PERFORMANCE PERFORMANCE OF EXPRESSION OF SUCH GENES, WHERE THE EXPRESSION PATTERN OF A RENAL SAMPLE FOR CHALLENGE BY ISA IS COMPARED, WITH THE EXPRESSION PATTERN OF SUCH GENES, IN A SAMPLE CONTROL WITHOUT CHALLENGE, AND THE NON-PRESENCE OF A SIGNIFICANT STATISTICAL DIFFERENCE (P <0.001), IS INDICATIVE OF AN EFFECTIVE VACCINE FOR PHENOTYPE SUSCEPTIBLE TO ISA, THAT IS, WITH A CHARACTERISTIC PRESENT, A MORTALITY OVER 50% FOR ISA VIRUS AND A HEREDABILITY OF SUCH OR EQUAL CHARACTER AT 0 , 34, AND WHERE THE REAL-TIME PCR TECHNIQUE IS CARRIED OUT WITH SELECTED PARTIES FROM THE GROUP CONSISTING OF: ANNEXES TO THE SEQUENCE SEQUENCE CTCCAGGAAATTGAACACCGCGA AND ANTI-SENSE SEQUENCE AAGGCTGCGATGAAGGACATGGT, IGZ SEQUENCE SEQUENCE AGCACCAGGGACAACCACCAT AND NON-SECURED SEQUENCE TTCACACTCGGTGGGTTAGAGTC AND REGUL F SEQUENCE SEQUENCE GACTCCTAACCTCCAATGCTTCGAC AND SEQUATTCATA SECCATCAT.

CL2013002736A 2013-09-25 2013-09-25 Use of annexin a1, regul and igz genes to determine the efficacy of a phenotype vaccine susceptible to infectious salmon anemia (isa) in Atlantic salmon (salmo salar) CL2013002736A1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CL2013002736A CL2013002736A1 (en) 2013-09-25 2013-09-25 Use of annexin a1, regul and igz genes to determine the efficacy of a phenotype vaccine susceptible to infectious salmon anemia (isa) in Atlantic salmon (salmo salar)
NO20141150A NO20141150A1 (en) 2013-09-25 2014-09-23 Use of the Annexin A1, Regul and Igz genes to determine the efficacy of a vaccine in phenotype susceptible to infectious salmon anemia in Atlantic salmon
CA2864728A CA2864728A1 (en) 2013-09-25 2014-09-25 Use of annexin a1, regul and igz to determine the effectiveness of a vaccine in phenotype susceptible to infectious salmon anemia (isa) in atlantic salmon (salmo salar)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CL2013002736A CL2013002736A1 (en) 2013-09-25 2013-09-25 Use of annexin a1, regul and igz genes to determine the efficacy of a phenotype vaccine susceptible to infectious salmon anemia (isa) in Atlantic salmon (salmo salar)

Publications (1)

Publication Number Publication Date
CL2013002736A1 true CL2013002736A1 (en) 2014-04-11

Family

ID=52737546

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2013002736A CL2013002736A1 (en) 2013-09-25 2013-09-25 Use of annexin a1, regul and igz genes to determine the efficacy of a phenotype vaccine susceptible to infectious salmon anemia (isa) in Atlantic salmon (salmo salar)

Country Status (3)

Country Link
CA (1) CA2864728A1 (en)
CL (1) CL2013002736A1 (en)
NO (1) NO20141150A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021123099A1 (en) * 2019-12-20 2021-06-24 Intervet International B.V. Reassorted isa virus
CN111678973A (en) * 2020-07-18 2020-09-18 上海海洋大学 Method for rapidly identifying Atlantic salmon and rainbow trout based on mass spectrometry technology

Also Published As

Publication number Publication date
NO20141150A1 (en) 2015-03-26
CA2864728A1 (en) 2015-03-25

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