CA3245930A1 - OPTIMIZATION OF THE HEK293 SUSPENSION PLATFORM FOR ENHANCED VAAR SECURITIES - Google Patents
OPTIMIZATION OF THE HEK293 SUSPENSION PLATFORM FOR ENHANCED VAAR SECURITIESInfo
- Publication number
- CA3245930A1 CA3245930A1 CA3245930A CA3245930A CA3245930A1 CA 3245930 A1 CA3245930 A1 CA 3245930A1 CA 3245930 A CA3245930 A CA 3245930A CA 3245930 A CA3245930 A CA 3245930A CA 3245930 A1 CA3245930 A1 CA 3245930A1
- Authority
- CA
- Canada
- Prior art keywords
- cells
- aav
- plasmid
- raav
- hek293
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14141—Use of virus, viral particle or viral elements as a vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14141—Use of virus, viral particle or viral elements as a vector
- C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14151—Methods of production or purification of viral material
- C12N2750/14152—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Virology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202263329856P | 2022-04-11 | 2022-04-11 | |
| US63/329,856 | 2022-04-11 | ||
| US202263331438P | 2022-04-15 | 2022-04-15 | |
| US63/331,438 | 2022-04-15 | ||
| PCT/US2023/017838 WO2023200679A1 (en) | 2022-04-11 | 2023-04-07 | OPTIMIZATION OF HEK293 SUSPENSION PLATFORM FOR IMPROVED rAAV TITERS |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3245930A1 true CA3245930A1 (en) | 2023-10-19 |
Family
ID=88330208
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3245930A Pending CA3245930A1 (en) | 2022-04-11 | 2023-04-07 | OPTIMIZATION OF THE HEK293 SUSPENSION PLATFORM FOR ENHANCED VAAR SECURITIES |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US20250250547A1 (https=) |
| EP (1) | EP4508198A4 (https=) |
| JP (1) | JP2025512382A (https=) |
| KR (1) | KR20250004745A (https=) |
| CN (1) | CN119053692A (https=) |
| AU (1) | AU2023254746A1 (https=) |
| CA (1) | CA3245930A1 (https=) |
| MX (1) | MX2024012608A (https=) |
| WO (1) | WO2023200679A1 (https=) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4683041A1 (en) | 2024-02-29 | 2026-01-21 | LG Energy Solution, Ltd. | Method for regenerating cathode active material, and regenerated cathode active material prepared thereby |
| WO2026013235A1 (en) * | 2024-07-11 | 2026-01-15 | Ferring International Center S.A. | Methods of producing recombinant adenovirus vectors |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7208315B2 (en) * | 2000-01-05 | 2007-04-24 | Fred Hutchinson Cancer Research Center | Compositions and methods for efficient AAV vector production |
| US20140315294A1 (en) * | 2011-11-24 | 2014-10-23 | Genethon | Scalable lentiviral vector production system compatible with industrial pharmaceutical applications |
| CA3130108A1 (en) * | 2019-02-22 | 2020-08-27 | Life Technologies Corporation | Suspension system for adeno associated virus production |
-
2023
- 2023-04-07 AU AU2023254746A patent/AU2023254746A1/en active Pending
- 2023-04-07 WO PCT/US2023/017838 patent/WO2023200679A1/en not_active Ceased
- 2023-04-07 CA CA3245930A patent/CA3245930A1/en active Pending
- 2023-04-07 EP EP23788783.1A patent/EP4508198A4/en active Pending
- 2023-04-07 CN CN202380033234.3A patent/CN119053692A/zh active Pending
- 2023-04-07 KR KR1020247037355A patent/KR20250004745A/ko active Pending
- 2023-04-07 US US18/855,209 patent/US20250250547A1/en active Pending
- 2023-04-07 JP JP2024560323A patent/JP2025512382A/ja active Pending
-
2024
- 2024-10-10 MX MX2024012608A patent/MX2024012608A/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP4508198A1 (en) | 2025-02-19 |
| KR20250004745A (ko) | 2025-01-08 |
| EP4508198A4 (en) | 2026-04-22 |
| US20250250547A1 (en) | 2025-08-07 |
| WO2023200679A1 (en) | 2023-10-19 |
| JP2025512382A (ja) | 2025-04-17 |
| CN119053692A (zh) | 2024-11-29 |
| MX2024012608A (es) | 2024-11-08 |
| AU2023254746A1 (en) | 2024-10-10 |
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Legal Events
| Date | Code | Title | Description |
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| A00 | Application filed |
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