CA3237337A1 - Nouveaux systemes crispr-cas12i et leurs utilisations - Google Patents

Nouveaux systemes crispr-cas12i et leurs utilisations Download PDF

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Publication number
CA3237337A1
CA3237337A1 CA3237337A CA3237337A CA3237337A1 CA 3237337 A1 CA3237337 A1 CA 3237337A1 CA 3237337 A CA3237337 A CA 3237337A CA 3237337 A CA3237337 A CA 3237337A CA 3237337 A1 CA3237337 A1 CA 3237337A1
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Canada
Prior art keywords
cas12i
polypeptide
seq
sequence
activity
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Pending
Application number
CA3237337A
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English (en)
Inventor
Hainan ZHANG
Xiangfeng Kong
Qijia Chen
Jingxing ZHOU
Haoqiang WANG
Weihong Zhang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Huidagene Therapeutics Singapore Pte Ltd
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Huidagene Therapeutics Singapore Pte Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Huidagene Therapeutics Singapore Pte Ltd filed Critical Huidagene Therapeutics Singapore Pte Ltd
Publication of CA3237337A1 publication Critical patent/CA3237337A1/fr
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22Ribonucleases RNAses, DNAses
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/005Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'active' part of the composition delivered, i.e. the nucleic acid delivered
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102Mutagenizing nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/88Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using amphiphile liposome vesicle
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/10Type of nucleic acid
    • C12N2310/20Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)

Abstract

L'invention concerne des polypeptides Cas12i, des protéines de fusion comprenant de tels polypeptides Cas12i, des systèmes CRISPR-Cas12i comprenant de tels polypeptides Cas12i ou des protéines de fusion, et leurs méthodes d'utilisation.
CA3237337A 2021-11-02 2022-11-02 Nouveaux systemes crispr-cas12i et leurs utilisations Pending CA3237337A1 (fr)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
CN202111289092 2021-11-02
CN202111289092.6 2021-11-02
CN202210081981.1 2022-01-24
CN202210081981 2022-01-24
CNPCT/CN2022/089074 2022-04-25
CN2022089074 2022-04-25
PCT/CN2022/129376 WO2023078314A1 (fr) 2021-11-02 2022-11-02 Nouveaux systèmes crispr-cas12i et leurs utilisations

Publications (1)

Publication Number Publication Date
CA3237337A1 true CA3237337A1 (fr) 2023-05-11

Family

ID=86240684

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3237337A Pending CA3237337A1 (fr) 2021-11-02 2022-11-02 Nouveaux systemes crispr-cas12i et leurs utilisations

Country Status (6)

Country Link
EP (1) EP4305160A1 (fr)
KR (1) KR20240111314A (fr)
AU (1) AU2022382751A1 (fr)
CA (1) CA3237337A1 (fr)
IL (1) IL312553A (fr)
WO (1) WO2023078314A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023185697A2 (fr) * 2022-03-29 2023-10-05 Accuredit Therapeutics (Suzhou) Co., Ltd. Compositions et méthodes pour le traitement de l'amyloïdose de la transthyrétine
CN117683749B (zh) * 2024-02-04 2024-05-17 广州瑞风生物科技有限公司 Cas蛋白及其应用

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE202019005567U1 (de) * 2018-03-14 2021-02-16 Arbor Biotechnologies, Inc. Neue CRISPR-DNA-Targeting-Enzyme und -Systeme
SG11202104347UA (en) * 2018-10-29 2021-05-28 Univ China Agricultural Novel crispr/cas12f enzyme and system
CN113308451B (zh) * 2020-12-07 2023-07-25 中国科学院动物研究所 工程化的Cas效应蛋白及其使用方法
CN113151215B (zh) * 2021-05-27 2022-11-18 中国科学院动物研究所 工程化的Cas12i核酸酶及其效应蛋白以及用途

Also Published As

Publication number Publication date
KR20240111314A (ko) 2024-07-16
WO2023078314A1 (fr) 2023-05-11
IL312553A (en) 2024-07-01
AU2022382751A1 (en) 2024-05-23
EP4305160A1 (fr) 2024-01-17

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