CA3227004A1 - Editeurs primaires ameliores et leurs procedes d'utilisation - Google Patents
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
-
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
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- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
- C12Y207/07049—RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/35—Nature of the modification
- C12N2310/351—Conjugate
- C12N2310/3519—Fusion with another nucleic acid
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
La présente divulgation concerne des compositions et des procédés d'édition primaire présentant une efficacité d'édition améliorée et/ou une formation d'indel réduite à l'aide d'éditeurs primaires modifiés et de protéines de fusion d'éditeur primaire. La divulgation concerne en outre des vecteurs, des cellules et des kits comprenant les compositions et les polynucléotides de la divulgation.
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
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US202163230688P | 2021-08-06 | 2021-08-06 | |
US63/230,688 | 2021-08-06 | ||
US202263388888P | 2022-07-13 | 2022-07-13 | |
US63/388,888 | 2022-07-13 | ||
PCT/US2022/074628 WO2023015309A2 (fr) | 2021-08-06 | 2022-08-05 | Éditeurs primaires améliorés et leurs procédés d'utilisation |
Publications (1)
Publication Number | Publication Date |
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CA3227004A1 true CA3227004A1 (fr) | 2023-02-09 |
Family
ID=83188605
Family Applications (1)
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CA3227004A Pending CA3227004A1 (fr) | 2021-08-06 | 2022-08-05 | Editeurs primaires ameliores et leurs procedes d'utilisation |
Country Status (5)
Country | Link |
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EP (1) | EP4381057A2 (fr) |
JP (1) | JP2024530487A (fr) |
AU (1) | AU2022325166A1 (fr) |
CA (1) | CA3227004A1 (fr) |
WO (1) | WO2023015309A2 (fr) |
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EP4399292A2 (fr) | 2021-09-08 | 2024-07-17 | Flagship Pioneering Innovations VI, LLC | Procédés et compositions pour moduler un génome |
WO2024077267A1 (fr) | 2022-10-07 | 2024-04-11 | The Broad Institute, Inc. | Méthodes et compositions d'édition d'amorce pour traiter des troubles de répétition de triplet |
Family Cites Families (38)
Publication number | Priority date | Publication date | Assignee | Title |
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US4217344A (en) | 1976-06-23 | 1980-08-12 | L'oreal | Compositions containing aqueous dispersions of lipid spheres |
US4235871A (en) | 1978-02-24 | 1980-11-25 | Papahadjopoulos Demetrios P | Method of encapsulating biologically active materials in lipid vesicles |
US4186183A (en) | 1978-03-29 | 1980-01-29 | The United States Of America As Represented By The Secretary Of The Army | Liposome carriers in chemotherapy of leishmaniasis |
US4261975A (en) | 1979-09-19 | 1981-04-14 | Merck & Co., Inc. | Viral liposome particle |
US4485054A (en) | 1982-10-04 | 1984-11-27 | Lipoderm Pharmaceuticals Limited | Method of encapsulating biologically active materials in multilamellar lipid vesicles (MLV) |
US4501728A (en) | 1983-01-06 | 1985-02-26 | Technology Unlimited, Inc. | Masking of liposomes from RES recognition |
US4897355A (en) | 1985-01-07 | 1990-01-30 | Syntex (U.S.A.) Inc. | N[ω,(ω-1)-dialkyloxy]- and N-[ω,(ω-1)-dialkenyloxy]-alk-1-yl-N,N,N-tetrasubstituted ammonium lipids and uses therefor |
US4946787A (en) | 1985-01-07 | 1990-08-07 | Syntex (U.S.A.) Inc. | N-(ω,(ω-1)-dialkyloxy)- and N-(ω,(ω-1)-dialkenyloxy)-alk-1-yl-N,N,N-tetrasubstituted ammonium lipids and uses therefor |
US5049386A (en) | 1985-01-07 | 1991-09-17 | Syntex (U.S.A.) Inc. | N-ω,(ω-1)-dialkyloxy)- and N-(ω,(ω-1)-dialkenyloxy)Alk-1-YL-N,N,N-tetrasubstituted ammonium lipids and uses therefor |
US4774085A (en) | 1985-07-09 | 1988-09-27 | 501 Board of Regents, Univ. of Texas | Pharmaceutical administration systems containing a mixture of immunomodulators |
US4837028A (en) | 1986-12-24 | 1989-06-06 | Liposome Technology, Inc. | Liposomes with enhanced circulation time |
US5244797B1 (en) | 1988-01-13 | 1998-08-25 | Life Technologies Inc | Cloned genes encoding reverse transcriptase lacking rnase h activity |
US5264618A (en) | 1990-04-19 | 1993-11-23 | Vical, Inc. | Cationic lipids for intracellular delivery of biologically active molecules |
WO1991017424A1 (fr) | 1990-05-03 | 1991-11-14 | Vical, Inc. | Acheminement intracellulaire de substances biologiquement actives effectue a l'aide de complexes de lipides s'auto-assemblant |
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EP1235914A2 (fr) | 1999-11-24 | 2002-09-04 | Joseph Rosenecker | Polypeptides comprenant des multimeres de signaux de localisation nucleaire ou de domaines de transduction de proteine et utilisations de ces derniers pour transferer des molecules dans des cellules |
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EP2137300B1 (fr) | 2007-04-26 | 2011-10-26 | Ramot at Tel-Aviv University Ltd. | Cellules souches pluripotentes autologues issues de la muqueuse buccale ou gastro-intestinale |
WO2010028347A2 (fr) | 2008-09-05 | 2010-03-11 | President & Fellows Of Harvard College | Evolution dirigée continue de protéines et d'acides nucléiques |
EP3415621A1 (fr) | 2009-03-04 | 2018-12-19 | Board Of Regents The University Of Texas System | Protéines de fusion de transcriptase inverse stabilisée |
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US9458484B2 (en) | 2010-10-22 | 2016-10-04 | Bio-Rad Laboratories, Inc. | Reverse transcriptase mixtures with improved storage stability |
AU2011348204B2 (en) | 2010-12-22 | 2017-03-02 | President And Fellows Of Harvard College | Continuous directed evolution |
JP6177780B2 (ja) | 2011-09-28 | 2017-08-09 | エラ、ビオテック、ソシエダッド、アノニマEra Biotech, S.A. | スプリットインテインおよびその使用 |
DK3431497T3 (da) | 2012-06-27 | 2022-10-10 | Univ Princeton | Spaltede inteiner, konjugater og anvendelser deraf |
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JO3470B1 (ar) | 2012-10-08 | 2020-07-05 | Merck Sharp & Dohme | مشتقات 5- فينوكسي-3h-بيريميدين-4-أون واستخدامها كمثبطات ناسخ عكسي ل hiv |
US9737604B2 (en) | 2013-09-06 | 2017-08-22 | President And Fellows Of Harvard College | Use of cationic lipids to deliver CAS9 |
US10179911B2 (en) | 2014-01-20 | 2019-01-15 | President And Fellows Of Harvard College | Negative selection and stringency modulation in continuous evolution systems |
CN107075491B (zh) | 2014-10-28 | 2021-07-06 | 谷万达公司 | 用于稳定反式剪接的内含肽修饰的蛋白酶的方法和组合物 |
WO2016168631A1 (fr) | 2015-04-17 | 2016-10-20 | President And Fellows Of Harvard College | Système de mutagénèse à base de vecteurs |
US9580698B1 (en) | 2016-09-23 | 2017-02-28 | New England Biolabs, Inc. | Mutant reverse transcriptase |
US20200140835A1 (en) * | 2017-06-06 | 2020-05-07 | The General Hospital Corporation | Engineered CRISPR-Cas9 Nucleases |
WO2019092042A1 (fr) * | 2017-11-10 | 2019-05-16 | Novozymes A/S | Protéine cas9 sensible à la température |
WO2020191249A1 (fr) * | 2019-03-19 | 2020-09-24 | The Broad Institute, Inc. | Procédés et compositions pour l'édition de séquences nucléotidiques |
WO2021080922A1 (fr) * | 2019-10-21 | 2021-04-29 | The Trustees Of Columbia University In The City Of New York | Procédés de mise en oeuvre d'édition de génome à matrice d'arn |
CN116209756A (zh) * | 2020-03-04 | 2023-06-02 | 旗舰先锋创新Vi有限责任公司 | 调控基因组的方法和组合物 |
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2022
- 2022-08-05 JP JP2024507108A patent/JP2024530487A/ja active Pending
- 2022-08-05 EP EP22764605.6A patent/EP4381057A2/fr active Pending
- 2022-08-05 WO PCT/US2022/074628 patent/WO2023015309A2/fr active Application Filing
- 2022-08-05 CA CA3227004A patent/CA3227004A1/fr active Pending
- 2022-08-05 AU AU2022325166A patent/AU2022325166A1/en active Pending
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AU2022325166A1 (en) | 2024-02-08 |
EP4381057A2 (fr) | 2024-06-12 |
JP2024530487A (ja) | 2024-08-21 |
WO2023015309A3 (fr) | 2023-03-16 |
WO2023015309A2 (fr) | 2023-02-09 |
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