CA3224264A1 - Aptamer dynamic range compression and detection techniques - Google Patents
Aptamer dynamic range compression and detection techniques Download PDFInfo
- Publication number
- CA3224264A1 CA3224264A1 CA3224264A CA3224264A CA3224264A1 CA 3224264 A1 CA3224264 A1 CA 3224264A1 CA 3224264 A CA3224264 A CA 3224264A CA 3224264 A CA3224264 A CA 3224264A CA 3224264 A1 CA3224264 A1 CA 3224264A1
- Authority
- CA
- Canada
- Prior art keywords
- aptamer
- probe
- region
- probes
- sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/682—Signal amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6839—Triple helix formation or other higher order conformations in hybridisation assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (9)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202263329101P | 2022-04-08 | 2022-04-08 | |
| US63/329,101 | 2022-04-08 | ||
| US202263343760P | 2022-05-19 | 2022-05-19 | |
| US63/343,760 | 2022-05-19 | ||
| US202263347375P | 2022-05-31 | 2022-05-31 | |
| US63/347,375 | 2022-05-31 | ||
| US202263385544P | 2022-11-30 | 2022-11-30 | |
| US63/385,544 | 2022-11-30 | ||
| PCT/US2023/017778 WO2023196528A1 (en) | 2022-04-08 | 2023-04-06 | Aptamer dynamic range compression and detection techniques |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3224264A1 true CA3224264A1 (en) | 2023-10-12 |
Family
ID=86282560
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3224264A Pending CA3224264A1 (en) | 2022-04-08 | 2023-04-06 | Aptamer dynamic range compression and detection techniques |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20240287583A1 (enExample) |
| EP (1) | EP4504966A1 (enExample) |
| JP (1) | JP2025512974A (enExample) |
| AU (1) | AU2023250540A1 (enExample) |
| CA (1) | CA3224264A1 (enExample) |
| WO (1) | WO2023196528A1 (enExample) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2025178951A1 (en) * | 2024-02-22 | 2025-08-28 | Illumina, Inc. | Techniques for dynamic range compression grouping in analyte assays |
| WO2026073207A1 (en) | 2024-09-30 | 2026-04-02 | Illumina, Inc. | Affinity-binder-based assay compositions and methods |
| WO2026143014A1 (en) | 2024-12-23 | 2026-07-02 | Illumina, Inc. | Affinity-binder-based assay compositions and methods for spatial proteomics |
| WO2026143006A2 (en) | 2024-12-23 | 2026-07-02 | Illumina, Inc. | Aptamer-based assay compositions and methods |
Family Cites Families (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5846719A (en) | 1994-10-13 | 1998-12-08 | Lynx Therapeutics, Inc. | Oligonucleotide tags for sorting and identification |
| US5750341A (en) | 1995-04-17 | 1998-05-12 | Lynx Therapeutics, Inc. | DNA sequencing by parallel oligonucleotide extensions |
| EP2327797B1 (en) | 1997-04-01 | 2015-11-25 | Illumina Cambridge Limited | Method of nucleic acid sequencing |
| US6969488B2 (en) | 1998-05-22 | 2005-11-29 | Solexa, Inc. | System and apparatus for sequential processing of analytes |
| US7001792B2 (en) | 2000-04-24 | 2006-02-21 | Eagle Research & Development, Llc | Ultra-fast nucleic acid sequencing device and a method for making and using the same |
| WO2002031206A2 (en) * | 2000-10-11 | 2002-04-18 | Ragland William L | Methods and compositions using hybridization assays for detecting infectious agents |
| US7057026B2 (en) | 2001-12-04 | 2006-06-06 | Solexa Limited | Labelled nucleotides |
| AU2003259350A1 (en) | 2002-08-23 | 2004-03-11 | Solexa Limited | Modified nucleotides for polynucleotide sequencing |
| GB0321306D0 (en) | 2003-09-11 | 2003-10-15 | Solexa Ltd | Modified polymerases for improved incorporation of nucleotide analogues |
| EP2789383B1 (en) | 2004-01-07 | 2023-05-03 | Illumina Cambridge Limited | Molecular arrays |
| WO2006064199A1 (en) | 2004-12-13 | 2006-06-22 | Solexa Limited | Improved method of nucleotide detection |
| JP4990886B2 (ja) | 2005-05-10 | 2012-08-01 | ソレックサ リミテッド | 改良ポリメラーゼ |
| GB0514936D0 (en) | 2005-07-20 | 2005-08-24 | Solexa Ltd | Preparation of templates for nucleic acid sequencing |
| US7329860B2 (en) | 2005-11-23 | 2008-02-12 | Illumina, Inc. | Confocal imaging methods and apparatus |
| US8349167B2 (en) | 2006-12-14 | 2013-01-08 | Life Technologies Corporation | Methods and apparatus for detecting molecular interactions using FET arrays |
| EP2677309B9 (en) | 2006-12-14 | 2014-11-19 | Life Technologies Corporation | Methods for sequencing a nucleic acid using large scale FET arrays, configured to measure a limited pH range |
| US8262900B2 (en) | 2006-12-14 | 2012-09-11 | Life Technologies Corporation | Methods and apparatus for measuring analytes using large scale FET arrays |
| US7964356B2 (en) | 2007-01-16 | 2011-06-21 | Somalogic, Inc. | Method for generating aptamers with improved off-rates |
| US20110136099A1 (en) | 2007-01-16 | 2011-06-09 | Somalogic, Inc. | Multiplexed Analyses of Test Samples |
| US8975026B2 (en) | 2007-01-16 | 2015-03-10 | Somalogic, Inc. | Method for generating aptamers with improved off-rates |
| US7855054B2 (en) * | 2007-01-16 | 2010-12-21 | Somalogic, Inc. | Multiplexed analyses of test samples |
| EP2121983A2 (en) | 2007-02-02 | 2009-11-25 | Illumina Cambridge Limited | Methods for indexing samples and sequencing multiple nucleotide templates |
| US20100137143A1 (en) | 2008-10-22 | 2010-06-03 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes |
| US10066257B2 (en) * | 2010-03-29 | 2018-09-04 | Dxterity Diagnostics Incorporated | Methods and compositions for detecting target nucleic acids |
| US9074251B2 (en) | 2011-02-10 | 2015-07-07 | Illumina, Inc. | Linking sequence reads using paired code tags |
| CA2821299C (en) | 2010-11-05 | 2019-02-12 | Frank J. Steemers | Linking sequence reads using paired code tags |
| US8829171B2 (en) | 2011-02-10 | 2014-09-09 | Illumina, Inc. | Linking sequence reads using paired code tags |
| US9862995B2 (en) * | 2012-03-13 | 2018-01-09 | Abhijit Ajit Patel | Measurement of nucleic acid variants using highly-multiplexed error-suppressed deep sequencing |
| DK3368666T3 (da) * | 2015-10-30 | 2020-12-14 | Guangdong Maijinjia Biotechnologies Co Ltd | Fremgangsmåder til identifikation af oligonucleotider |
| WO2018005811A1 (en) * | 2016-06-30 | 2018-01-04 | Grail, Inc. | Differential tagging of rna for preparation of a cell-free dna/rna sequencing library |
| CN110295231A (zh) * | 2017-12-20 | 2019-10-01 | 威廉马歇莱思大学 | 通过选择性等位基因富集或消耗用低深度测序检测和定量稀有变体 |
| JP7372927B6 (ja) * | 2018-02-12 | 2023-11-27 | ナノストリング テクノロジーズ，インコーポレイティド | 遺伝子およびタンパク質の発現を検出する生体分子プローブおよびその検出方法 |
-
2023
- 2023-04-06 JP JP2024559275A patent/JP2025512974A/ja active Pending
- 2023-04-06 AU AU2023250540A patent/AU2023250540A1/en active Pending
- 2023-04-06 WO PCT/US2023/017778 patent/WO2023196528A1/en not_active Ceased
- 2023-04-06 US US18/571,069 patent/US20240287583A1/en active Pending
- 2023-04-06 CA CA3224264A patent/CA3224264A1/en active Pending
- 2023-04-06 EP EP23720693.3A patent/EP4504966A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| WO2023196528A1 (en) | 2023-10-12 |
| AU2023250540A1 (en) | 2024-01-18 |
| EP4504966A1 (en) | 2025-02-12 |
| JP2025512974A (ja) | 2025-04-22 |
| US20240287583A1 (en) | 2024-08-29 |
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