CA3193980A1 - Modification d'un oligomere proteique formant un nanopore - Google Patents

Modification d'un oligomere proteique formant un nanopore

Info

Publication number
CA3193980A1
CA3193980A1 CA3193980A CA3193980A CA3193980A1 CA 3193980 A1 CA3193980 A1 CA 3193980A1 CA 3193980 A CA3193980 A CA 3193980A CA 3193980 A CA3193980 A CA 3193980A CA 3193980 A1 CA3193980 A1 CA 3193980A1
Authority
CA
Canada
Prior art keywords
monomer
pore
protein
monomers
modified
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3193980A
Other languages
English (en)
Inventor
Lakmal Nishantha JAYASINGHE
Elizabeth Jayne Wallace
Ranga Prabhath MALAVIARACHCHIGE RABEL
John Joseph KILGOUR
Kimberley Emma BLACKWELL
Pratik Raj SINGH
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Oxford Nanopore Technologies PLC
Original Assignee
Oxford Nanopore Technologies PLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oxford Nanopore Technologies PLC filed Critical Oxford Nanopore Technologies PLC
Publication of CA3193980A1 publication Critical patent/CA3193980A1/fr
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/1072—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/1072—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups
    • C07K1/1075—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups by covalent attachment of amino acids or peptide residues
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/1072—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups
    • C07K1/1077—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups by covalent attachment of residues other than amino acids or peptide residues, e.g. sugars, polyols, fatty acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/13—Labelling of peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/1031—Investigating individual particles by measuring electrical or magnetic effects
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N2015/0038—Investigating nanoparticles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1024—Counting particles by non-optical means

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Immunology (AREA)
  • Food Science & Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Dispersion Chemistry (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Nanotechnology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

L'invention concerne un procédé de modification chimique d'un polypeptide ou d'un monomère polynucléotidique. Le monomère peut être un monomère d'un pore protéique tel qu'un pore protéique monomère ou oligomère. L'invention concerne également des monomères et des oligomères associés et des procédés d'utilisation des pores décrits dans la caractérisation d'analytes.
CA3193980A 2020-10-08 2021-10-08 Modification d'un oligomere proteique formant un nanopore Pending CA3193980A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB2015993.5A GB202015993D0 (en) 2020-10-08 2020-10-08 Method
GB2015993.5 2020-10-08
PCT/GB2021/052609 WO2022074397A1 (fr) 2020-10-08 2021-10-08 Modification d'un oligomère protéique formant un nanopore

Publications (1)

Publication Number Publication Date
CA3193980A1 true CA3193980A1 (fr) 2022-04-14

Family

ID=73460466

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3193980A Pending CA3193980A1 (fr) 2020-10-08 2021-10-08 Modification d'un oligomere proteique formant un nanopore

Country Status (7)

Country Link
EP (1) EP4225768A1 (fr)
JP (1) JP2023545759A (fr)
CN (1) CN116323634A (fr)
AU (1) AU2021356235A1 (fr)
CA (1) CA3193980A1 (fr)
GB (1) GB202015993D0 (fr)
WO (1) WO2022074397A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB202112235D0 (en) * 2021-08-26 2021-10-13 Oxford Nanopore Tech Ltd Nanopore

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5198543A (en) 1989-03-24 1993-03-30 Consejo Superior Investigaciones Cientificas PHI29 DNA polymerase
US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
EP2126588A1 (fr) 2007-02-20 2009-12-02 Oxford Nanopore Technologies Limited Formation de bicouches lipidiques
WO2009020682A2 (fr) 2007-05-08 2009-02-12 The Trustees Of Boston University Fonctionnalisation chimique d'ensembles de nanopores et de nanopores à semi-conducteurs, et leurs applications
EP3540436B1 (fr) 2007-09-12 2023-11-01 President And Fellows Of Harvard College Capteur moléculaire haute résolution
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
CN102439043A (zh) 2009-01-30 2012-05-02 牛津纳米孔技术有限公司 杂交连接物
GB0901588D0 (en) 2009-02-02 2009-03-11 Itis Holdings Plc Apparatus and methods for providing journey information
EP2422198B1 (fr) 2009-04-20 2013-09-25 Oxford Nanopore Technologies Limited Réseau de capteurs de bicouche lipidique
EP2580588B1 (fr) 2010-06-08 2014-09-24 President and Fellows of Harvard College Dispositif à nanopores avec membrane lipidique artificielle sur support de graphène
EP4273092A3 (fr) 2011-05-27 2024-01-17 Oxford Nanopore Technologies plc Procédé et appareil permettant de déterminer la présence, l'absence ou les caractéristiques d'un analyte
BR112014009579B1 (pt) 2011-10-21 2021-06-22 Oxford Nanopore Technologies Limited Métodos para caracterizar um polinucleotídeo alvo, e para formar um sensor, uso de uma helicase, kit, e, aparelho de análise
GB201120910D0 (en) 2011-12-06 2012-01-18 Cambridge Entpr Ltd Nanopore functionality control
US10385382B2 (en) 2011-12-29 2019-08-20 Oxford Nanopore Technologies Ltd. Enzyme method
US9617591B2 (en) 2011-12-29 2017-04-11 Oxford Nanopore Technologies Ltd. Method for characterising a polynucleotide by using a XPD helicase
BR112014025157B1 (pt) 2012-04-10 2022-02-08 Oxford Nanopore Technologies Limited Monômero de lisenina mutante, construto, poro, método para caracterizar um analito alvo, uso de um poro, e, kit
CA2879261C (fr) 2012-07-19 2022-12-06 Oxford Nanopore Technologies Limited Helicases modifiees
WO2014064444A1 (fr) 2012-10-26 2014-05-01 Oxford Nanopore Technologies Limited Interfaces de gouttelettes
GB201313121D0 (en) 2013-07-23 2013-09-04 Oxford Nanopore Tech Ltd Array of volumes of polar medium
JP6677640B2 (ja) 2013-10-18 2020-04-08 オックスフォード ナノポール テクノロジーズ リミテッド 修飾酵素
WO2015110813A1 (fr) 2014-01-22 2015-07-30 Oxford Nanopore Technologies Limited Procédé de fixation d'une ou plusieurs protéines de liaison de polynucléotides dans un polynucléotide cible
WO2015150786A1 (fr) 2014-04-04 2015-10-08 Oxford Nanopore Technologies Limited Méthode de caractérisation d'un acide nucléique double brin au moyen d'un nano-pore et de molécules d'ancrage aux deux extrémités dudit acide nucléique
GB201417712D0 (en) 2014-10-07 2014-11-19 Oxford Nanopore Tech Ltd Method
EP3432935B1 (fr) * 2016-03-24 2022-02-23 Genia Technologies, Inc. Procédés et compositions de bioconjugaison spécifique d'un site utiles pour des systèmes nanoporeux
AU2017246690B2 (en) * 2016-04-06 2022-03-24 Oxford Nanopore Technologies Plc Mutant pore

Also Published As

Publication number Publication date
JP2023545759A (ja) 2023-10-31
CN116323634A (zh) 2023-06-23
AU2021356235A1 (en) 2023-04-13
WO2022074397A1 (fr) 2022-04-14
GB202015993D0 (en) 2020-11-25
EP4225768A1 (fr) 2023-08-16

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