CA3182834A1 - Systems and methods for rapid inactivation of sars-cov-2 by silicon nitride and aluminum nitride - Google Patents
Systems and methods for rapid inactivation of sars-cov-2 by silicon nitride and aluminum nitride Download PDFInfo
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- CA3182834A1 CA3182834A1 CA3182834A CA3182834A CA3182834A1 CA 3182834 A1 CA3182834 A1 CA 3182834A1 CA 3182834 A CA3182834 A CA 3182834A CA 3182834 A CA3182834 A CA 3182834A CA 3182834 A1 CA3182834 A1 CA 3182834A1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/06—Aluminium; Calcium; Magnesium; Compounds thereof
-
- A—HUMAN NECESSITIES
- A41—WEARING APPAREL
- A41D—OUTERWEAR; PROTECTIVE GARMENTS; ACCESSORIES
- A41D31/00—Materials specially adapted for outerwear
- A41D31/04—Materials specially adapted for outerwear characterised by special function or use
- A41D31/30—Antimicrobial, e.g. antibacterial
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Environmental Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- General Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Health & Medical Sciences (AREA)
- Inorganic Chemistry (AREA)
- Agronomy & Crop Science (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Textile Engineering (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Crystals, And After-Treatments Of Crystals (AREA)
- Braking Arrangements (AREA)
- Paints Or Removers (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
Various embodiments related to systems, methods, and articles for rapid inactivation of SARS-CoV-2 by silicon nitride and aluminum nitride are disclosed herein.
Description
SYSTEMS AND METHODS FOR RAPID INACTIVATION OF SARS-CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application claims priority to US
Provisional Application No.
63/045,355, filed June 29, 2020, the contents of which are entirely incorporated by reference herein.
FIELD
[0001] This application claims priority to US
Provisional Application No.
63/045,355, filed June 29, 2020, the contents of which are entirely incorporated by reference herein.
FIELD
[0002] The present disclosure generally relates to rapid inactivation of a virus, and in particular to systems and methods for rapid capture and inactivation of SARS-CoV-2 by silicon nitride and/or aluminum nitride.
BACKGROUND
BACKGROUND
[0003] The novel coronavirus, SARS-CoV-2, has led to a worldwide pandemic and raised interest in the surface-mediated transmission of viral diseases.
Respiratory aerosols and droplets, and contaminated surfaces facilitate viral spread from person to person leading to recommendations of social distancing, wearing of masks, hand-washing, and regular surface disinfection. Data suggest that the SARS-CoV-2 virus can remain viable on copper, plastic, steel, and cardboard surfaces for
Respiratory aerosols and droplets, and contaminated surfaces facilitate viral spread from person to person leading to recommendations of social distancing, wearing of masks, hand-washing, and regular surface disinfection. Data suggest that the SARS-CoV-2 virus can remain viable on copper, plastic, steel, and cardboard surfaces for
4-72 hours after contact, and up to 7-days on surgical masks. Viral persistence on these and other materials presents a risk for the social and nosocomial propagation of COVID-19, the disease caused by SARS-CoV-2. Current viral inactivation methods include the surface application of chemicals, such as a combination of ethanol with hydrogen peroxide or sodium hypochlorite. Irradiation of surfaces with ultraviolet light is another virus disinfection strategy. These and other proposed antiviral methodologies are ultimately limited by their toxicity to human cells. As a practical solution, surfaces that are safe for human contact and capable of spontaneously inactivating viruses are desirable to control the spread of viral diseases.
[0004] It is with these observations in mind, among others, that various aspects of the present disclosure were conceived and developed.
CA 03182834 2022- 12- 14 RECTIFIED SHEET (RULE 91) SUMMARY
[0004] It is with these observations in mind, among others, that various aspects of the present disclosure were conceived and developed.
CA 03182834 2022- 12- 14 RECTIFIED SHEET (RULE 91) SUMMARY
[0005] Provided herein are embodiments of a method for capturing and inactivating a SARS-CoV-2 virus by contacting the virus with an object comprising silicon nitride and/or aluminum nitride.
[0006] In some aspects, the object may comprise silicon nitride or aluminum nitride, wherein the silicon nitride or aluminum nitride successively binds (i.e. captures) and then inactivates the virus. For example, the silicon nitride or the aluminum nitride may be present on the surface of the object as a coating or may be incorporated into the object. In some examples, the silicon nitride is present at a concentration from about 1 wt.% to about 30 wt.%.
[0007] In some aspects, the object may contact the virus for at least one minute or for at least 30 minutes. For example, the virus may be at least 75%
inactivated after contact with the object for at least 1 minute. In some aspects, the object may comprise paper, cardboard, polymers, fabric, plastic, ceramic, stainless steel, and/or metal. In some aspects, the object is a protective gown, a body cover, a head cover, a shoe cover, a face mask, a face shield, an eye protector, gloves, a surgical gown, a surgical drape, or a cubicle curtain. In some additional aspects, the object is a face mask filter, a respirator filter, an air filtration filter, or an air ventilation filter. In even more aspects, the object is a knob, a handle, a railing, a bed frame, a bed tray, a table, a chair, an equipment rack, or a cart. In some additional aspects, the object may be composition such as a slurry, suspension, gel, paint, or toothpaste.
inactivated after contact with the object for at least 1 minute. In some aspects, the object may comprise paper, cardboard, polymers, fabric, plastic, ceramic, stainless steel, and/or metal. In some aspects, the object is a protective gown, a body cover, a head cover, a shoe cover, a face mask, a face shield, an eye protector, gloves, a surgical gown, a surgical drape, or a cubicle curtain. In some additional aspects, the object is a face mask filter, a respirator filter, an air filtration filter, or an air ventilation filter. In even more aspects, the object is a knob, a handle, a railing, a bed frame, a bed tray, a table, a chair, an equipment rack, or a cart. In some additional aspects, the object may be composition such as a slurry, suspension, gel, paint, or toothpaste.
[0008] Also provided herein are embodiments of an article of personal protective equipment (PPE) having antiviral and antimicrobial properties. In some aspects, the article comprises silicon nitride or aluminum nitride. The silicon nitride or the aluminum nitride may be incorporated into the article or may be coated onto the surface of the article. In some aspects, the silicon nitride or aluminum nitride may be present at a concentration of about 1 wt.% to about 30 wt.%. In some aspects, the article may be a body cover, a head cover, a shoe cover, a face mask, a face and eye protector, or gloves. In some examples, the article is operable to bind/capture and then inactivate the SARS-CoV-2 virus upon contact with the virus.
BRIEF DESCRIPTION OF THE DRAWINGS
BRIEF DESCRIPTION OF THE DRAWINGS
[0009] FIGS. 1A-1D are graphical representations that show the inactivation of SARS-CoV-2 by nitride powders treated with 15 wt.% Cu, AIN, and Si3N4 powders in an aqueous medium at room temperature for 1- and 10-minutes in which the control virus was treated identically without addition of any powders. After centrifugation, supernatants were subjected to TCID5o assay. The Reed-Muench method was used to determine the virus titers. TCID5o/50 pL (FIGS. 1A and 1B) and % reduction FIGS. 1C and 1D are shown for virus inactivation times of 1 minutes and minutes, respectively. Statistics are given in the inset according to unpaired two-tailed Student's t-test (n=3).
[0010] FIGS. 2A-2D are graphical representations that show viral RNA
that has underwent severe degradation after exposure to copper or nitride particles.
In FIG. 2A and FIG. 2B, virus suspensions were exposed to Cu, AIN and Si3N4 powders for 1 minute, and viral RNA in supernatants and on particles were evaluated using viral N gene "set 1" and "set 2" primers, respectively. Data collected on supernatants and pellet samples are given in comparison with the amount of viral N
gene RNA in suspension that was left untreated. In FIG. 2C and FIG. 2D, results of RT-PCR tests after 10-minute exposure of supernatants to Cu, AIN and Si3N4 powders for viral N gene "set 1" and "set 2" primers are shown, respectively.
Statistics are given in the inset according to unpaired two-tailed Student's t-test (n=3).
that has underwent severe degradation after exposure to copper or nitride particles.
In FIG. 2A and FIG. 2B, virus suspensions were exposed to Cu, AIN and Si3N4 powders for 1 minute, and viral RNA in supernatants and on particles were evaluated using viral N gene "set 1" and "set 2" primers, respectively. Data collected on supernatants and pellet samples are given in comparison with the amount of viral N
gene RNA in suspension that was left untreated. In FIG. 2C and FIG. 2D, results of RT-PCR tests after 10-minute exposure of supernatants to Cu, AIN and Si3N4 powders for viral N gene "set 1" and "set 2" primers are shown, respectively.
Statistics are given in the inset according to unpaired two-tailed Student's t-test (n=3).
[0011] FIGS. 3A-3E are images showing Si3N4 suppressed virus infection without affecting cell viability in which Cu killed the cells.
VeroE6/TMPRSS2 cells were inoculated with (FIG. 3A) unexposed virions, and virions 10-minute UTE-exposed to Si3N4 (FIG. 3B), AIN (FIG. 3C), and Cu (FIG. 3D). In FIG. 3E, non-inoculated cells (labeled as "sham-infected" cells) were also prepared and imaged for comparison. After fixation, cells were stained with anti-SARS coronavirus envelope antibody (red), Phalloidin to visualize F-actin (green), and DAPI to stain nuclei (blue). Fluorescence micrographs are shown, which are representative of n=3 samples.
VeroE6/TMPRSS2 cells were inoculated with (FIG. 3A) unexposed virions, and virions 10-minute UTE-exposed to Si3N4 (FIG. 3B), AIN (FIG. 3C), and Cu (FIG. 3D). In FIG. 3E, non-inoculated cells (labeled as "sham-infected" cells) were also prepared and imaged for comparison. After fixation, cells were stained with anti-SARS coronavirus envelope antibody (red), Phalloidin to visualize F-actin (green), and DAPI to stain nuclei (blue). Fluorescence micrographs are shown, which are representative of n=3 samples.
[0012] FIG. 4 is a graphical representation showing fluorescently labeled and non-labeled cells were counted on fluorescence micrographs, and %
infected cells and % viable cells calculated as follows: % infected cells =
(number of the cells stained with anti-SARS coronavirus envelope antibody) / (number of cells stained with DAPI) x 100; and, % viable cells = (number of the cells stained with Phalloidin) / (number of cells stained with DAPI) x 100. Data is representative of n=3 samples. * and ** are p<0.05 and 0.01, respectively, by unpaired two-tailed Student's t-test (n=3); n.s. = non-significant.
infected cells and % viable cells calculated as follows: % infected cells =
(number of the cells stained with anti-SARS coronavirus envelope antibody) / (number of cells stained with DAPI) x 100; and, % viable cells = (number of the cells stained with Phalloidin) / (number of cells stained with DAPI) x 100. Data is representative of n=3 samples. * and ** are p<0.05 and 0.01, respectively, by unpaired two-tailed Student's t-test (n=3); n.s. = non-significant.
[0013] FIGS. 5A-5G are graphical representations of Raman spectra for: (a) uninfected cells (FIG. 5A) (i.e., unexposed to virions), and cells infected with SARS-CoV-2 virions exposed for 10 minutes to (b) Si31\14 (FIG. 5B), (c) AIN
(FIG.
5C), and (d) Cu (FIG. 5D); in FIG. 5E, Raman spectrum of cells infected by unexposed virions (negative control). In FIG. 5F, a plot of the average intensity of the two tryptophan T1 and T2 bands (at 756 and 875 cm-1, respectively) as a function of fraction of infected cells by virions unexposed and exposed for 10-min to different particles (cf. labels); in the inset, the structure of N'-formylkynurenine, an intermediate in the catabolism of tryptophan upon enzymatic IDO reaction. In FIG.
5G, a graphical representation shows three possible conformations of tyrosine-based peptides that can justify the disappearance of ring vibrations in tyrosine (Ty2 band) upon chelation of Cu(II) ions.
(FIG.
5C), and (d) Cu (FIG. 5D); in FIG. 5E, Raman spectrum of cells infected by unexposed virions (negative control). In FIG. 5F, a plot of the average intensity of the two tryptophan T1 and T2 bands (at 756 and 875 cm-1, respectively) as a function of fraction of infected cells by virions unexposed and exposed for 10-min to different particles (cf. labels); in the inset, the structure of N'-formylkynurenine, an intermediate in the catabolism of tryptophan upon enzymatic IDO reaction. In FIG.
5G, a graphical representation shows three possible conformations of tyrosine-based peptides that can justify the disappearance of ring vibrations in tyrosine (Ty2 band) upon chelation of Cu(II) ions.
[0014] FIG. 6 is a schematic model illustrating a chemical and electrical charge similarity between the protonated amine groups, Si¨NH3+, at the surface of Si3N4 and the N-terminal of lysine, C¨NH3+ in cells (left panel); and, the interaction of SARS-CoV-2 viruses with the charged molecular species at the surface of Si3N4 (specifically, at protonated amines charging plus) and the eluted species NH3/NI-14+
(central panel). The eluted N leaves 3+ charged vacancies on the solid surface (violet-colored sites), which stem together with negatively charged silanols.
The three-step process leading to RNA backbone cleavage by the eluted nitrogen species (namely, deprotonation of 2'-hydroxyl groups, formation of a transient pentaphosphate, and cleavage of the phosphodiester bond in the RNA backbone by alkaline transesterification through hydrolysis) is shown in the right panel.
Note that the similarity between protonated amine and N-terminal of lysine might trigger an extremely effective "competitive binding" mechanism for SARS-CoV-2 virion inactivation, while eluted ammonia fatally degrades the virion RNA in a combined "catch and kill" effect.
(central panel). The eluted N leaves 3+ charged vacancies on the solid surface (violet-colored sites), which stem together with negatively charged silanols.
The three-step process leading to RNA backbone cleavage by the eluted nitrogen species (namely, deprotonation of 2'-hydroxyl groups, formation of a transient pentaphosphate, and cleavage of the phosphodiester bond in the RNA backbone by alkaline transesterification through hydrolysis) is shown in the right panel.
Note that the similarity between protonated amine and N-terminal of lysine might trigger an extremely effective "competitive binding" mechanism for SARS-CoV-2 virion inactivation, while eluted ammonia fatally degrades the virion RNA in a combined "catch and kill" effect.
[0015] Corresponding reference characters indicate elements among the view of the drawings. The headings used in the figures do not limit the scope of the claims.
DETAILED DESCRIPTION
DETAILED DESCRIPTION
[0016] Various embodiments of the disclosure are discussed in detail below. While specific implementations are discussed, it should be understood that this is done for illustration purposes only. A person skilled in the relevant art will recognize that other components and configurations may be used without parting from the spirit and scope of the disclosure. Thus, the following description and drawings are illustrative and are not to be construed as limiting. Numerous specific details are described to provide a thorough understanding of the disclosure.
However, in certain instances, well-known or conventional details are not described in order to avoid obscuring the description.
However, in certain instances, well-known or conventional details are not described in order to avoid obscuring the description.
[0017] Several definitions that apply throughout this disclosure will now be presented. Reference to "one embodiment" or "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one embodiment of the disclosure. The appearances of the phrase "in one embodiment" in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments. Moreover, various features are described which may be exhibited by some embodiments and not by others.
[0018] As used herein, the terms "comprising," "having,"
and "including"
are used in their open, non-limiting sense. The terms "a," "an," and "the" are understood to encompass the plural as well as the singular. Thus, the term "a mixture thereof" also relates to "mixtures thereof."
and "including"
are used in their open, non-limiting sense. The terms "a," "an," and "the" are understood to encompass the plural as well as the singular. Thus, the term "a mixture thereof" also relates to "mixtures thereof."
[0019] As used herein, "about" refers to numeric values, including whole numbers, fractions, percentages, etc., whether or not explicitly indicated. The term "about" generally refers to a range of numerical values, for instance, 0.5-1%, 1-5% or 5-10% of the recited value, that one would consider equivalent to the recited value, for example, having the same function or result.
[0020] As used herein, the term "silicon nitride"
includes a-Si3N4, 3-Si3N4, SiYAION, 13-SiYAION, SiYON, SiAION, or combinations thereof.
includes a-Si3N4, 3-Si3N4, SiYAION, 13-SiYAION, SiYON, SiAION, or combinations thereof.
[0021] As used herein, "inactivate" or "inactivation"
refers to viral inactivation in which the virus is stopped from contaminating the product or subject either by removing virus completely or rendering them non-infectious.
refers to viral inactivation in which the virus is stopped from contaminating the product or subject either by removing virus completely or rendering them non-infectious.
[0022] The terms "object", "apparatus" or "component" as used herein include materials, compositions, devices, surface coatings, and/or composites.
In some examples the apparatus may include various medical devices or equipment, examination tables, clothing, filters, personal protective equipment such as masks and gloves, catheters, endoscopic instruments, commonly-touched surfaces where viral persistence may encourage the spread of disease, and the like. The apparatus may be metallic, polymeric, and/or ceramic (ex. silicon nitride and/or other ceramic materials).
In some examples the apparatus may include various medical devices or equipment, examination tables, clothing, filters, personal protective equipment such as masks and gloves, catheters, endoscopic instruments, commonly-touched surfaces where viral persistence may encourage the spread of disease, and the like. The apparatus may be metallic, polymeric, and/or ceramic (ex. silicon nitride and/or other ceramic materials).
[0023] As used herein, "contact" means in physical contact or within close enough proximity to a composition or apparatus to be affected by the composition or apparatus.
[0024] As used herein, "personal protective equipment" or "PPE"
means any device, article, or apparatus worn or otherwise used by a person to minimize exposure to pathogens or other harmful substances. Non-limiting examples of PPE include body covers, head covers, shoe covers, face masks, eye protectors, face and eye protectors, and gloves.
means any device, article, or apparatus worn or otherwise used by a person to minimize exposure to pathogens or other harmful substances. Non-limiting examples of PPE include body covers, head covers, shoe covers, face masks, eye protectors, face and eye protectors, and gloves.
[0025] The terms used in this specification generally have their ordinary meanings in the art, within the context of the disclosure, and in the specific context where each term is used. Alternative language and synonyms may be used for any one or more of the terms discussed herein, and no special significance should be placed upon whether or not a term is elaborated or discussed herein. In some cases, synonyms for certain terms are provided. A recital of one or more synonyms does not exclude the use of other synonyms. The use of examples anywhere in this specification including examples of any terms discussed herein is illustrative only, and is not intended to further limit the scope and meaning of the disclosure or of any example term. Likewise, the disclosure is not limited to various embodiments given in this specification.
[0026] Additional features and advantages of the disclosure will be set forth in the description which follows, and in part will be obvious from the description, or can be learned by practice of the herein disclosed principles. The features and advantages of the disclosure can be realized and obtained by means of the instruments and combinations particularly pointed out in the appended claims.
These and other features of the disclosure will become more fully apparent from the following description and appended claims, or can be learned by the practice of the principles set forth herein.
These and other features of the disclosure will become more fully apparent from the following description and appended claims, or can be learned by the practice of the principles set forth herein.
[0027] Provided herein is a method for inactivating the SARS-CoV-2 virus by contacting the virus with an object or composition comprising silicon nitride and/or aluminum nitride. The silicon nitride and/or aluminum nitride successively binds (i.e. captures) and then inactivates the virus (e.g. "catch and kill").
[0028] Silicon nitride possesses a unique surface chemistry which is biocompatible and provides a number of biomedical applications including 1) concurrent osteogenesis, osteoinduction, osteoconduction, and bacteriostasis, such as in spinal and dental implants; 2) killing of both gram-positive and gram-negative bacteria according to different mechanisms; 3) inactivation of human and animal viruses, bacteria, and fungi; and 4) polymer- or metal-matrix composites, natural or manmade fibers, polymers, or metals containing silicon nitride powder retain key silicon nitride bone restorative, bacteriostatic, antiviral, and antifungal properties.
[0029] Silicon nitride (Si3N4) is a non-oxide ceramic compound that has been used in many industries since the 1950s. A formulation of Si3N4 is FDA-cleared for use as an intervertebral spinal spacer in cervical and lumbar spine fusion surgery, with proven long-term safety, efficacy, and biocompatibility. Clinical data for Si3N4 implants compare favorably with other spine biomaterials, such as allograft, titanium, and polyetheretherketone. A curious finding is that Si3N4 implants have a lower incidence of bacterial infection (Le., less than 0.006%) when compared to other implant materials (2.7% to 18%). This property reflects the complex surface biochemistry of Si3N4 that elutes minute amounts of nitrogen, which is converted to ammonia, ammonium, and other reactive nitrogen species (RNS) that inhibit bacteria. A recent investigation also found that viral exposure to sintered Si3N4 powders in aqueous suspension neutralized H1N1 (Influenza A/Puerto Rico/8/1934), Feline calicivirus, and Enterovirus (EV-A71). Based on these findings, Si3N4 may be able to inactivate SARS-CoV-2.
[0030] Silicon nitride may be antipathogenic due to release of nitrogen containing species when in contact with an aqueous medium, or biologic fluids and tissues. The surface chemistry of silicon nitride may be shown as follows:
Si3N4 + 6H20 3Si02 + 4NH3 SiO2 + 2H20 Si(OH)4
Si3N4 + 6H20 3Si02 + 4NH3 SiO2 + 2H20 Si(OH)4
[0031] Nitrogen elutes faster (within minutes) than silicon because surface silanols are relatively stable. For viruses, it was surprisingly found that silicon nitride may provide for RNA cleavage via alkaline transesterification which leads to loss in genome integrity and virus inactivation. This may also reduce the activity of hemagglutinin. The elution of ammonia, along with an attendant increase in pH, inactivates viruses, bacteria, and fungi. As shown in the examples, it was surprisingly found that each of silicon nitride and aluminum nitride inactivates SARS-CoV-2.
[0032] The use of copper (Cu), a historically-recognized viricidal agent, is limited by its cell toxicity. In contrast to Cu, ceramic devices or apparatuses made of Si3N4 are biocompatible and not toxic to the human body. An advantage of Si3N4 is the versatility of the material; thus Si3N4 may be incorporated into polymers, bioactive glasses, and even other ceramics to create composites and coatings that retain the favorable biocompatible and antiviral properties of Si3N4.
[0033] The present disclosure compares the effects of exposing SARS-CoV-2 to aqueous suspensions of Si3N4 and aluminum nitride (AIN) particles and two controls, (i.e., a suspension of copper (Cu) particles (positive control) and a sham suspension of SARS-CoV-2 virions without any antiviral agent (negative control)).
Copper (Cu) was chosen as a positive control because of its well-known ability to inactivate a variety of microbes, including viruses. Aluminum nitride was included in the testing because, like Si3N4, it is a nitrogen-based compound whose surface hydrolysis in aqueous solution leads to the elution of nitrogen, with an attendant increase in pH. Since comparable antiviral and antibacterial phenomena are believed to be operative for all nitride-based compounds, AIN was used to provide additional insight into the antipathogenic mechanisms of nitrogen-containing inorganic materials.
Copper (Cu) was chosen as a positive control because of its well-known ability to inactivate a variety of microbes, including viruses. Aluminum nitride was included in the testing because, like Si3N4, it is a nitrogen-based compound whose surface hydrolysis in aqueous solution leads to the elution of nitrogen, with an attendant increase in pH. Since comparable antiviral and antibacterial phenomena are believed to be operative for all nitride-based compounds, AIN was used to provide additional insight into the antipathogenic mechanisms of nitrogen-containing inorganic materials.
[0034] The persistence of human coronaviruses on common materials (e.g., metal, plastic, paper, and fabric) and touch surfaces (e.g., knobs, handles, railings, tables, and desktops) can contribute to the nosocomial and social spread of disease. Wames et al. reported that at room temperature with 30%-40% humidity, the pathogenic human coronavirus 229E (HuCoV-229E) remained infectious in a lung cell model after at least 5 days of persistent viability on a variety of materials, such as Teflon, polyvinyl chloride, ceramic tile, glass, stainless steel, and silicone rubber. These investigators also showed rapid HuCoV-229E inactivation (within a few minutes) for simulated fingertip contamination on Cu surfaces. Cu ion release and the generation of reactive oxygen species (ROS) were involved in viral inactivation;
and increased contact time with copper and brass surfaces led to greater non-specific fragmentation of viral RNA, indicating irreversible viral inactivation. More recently, Doremalen et al. showed surface stability of both SARS-CoV-1 and SARS-CoV-2 virus on plastic, cardboard, stainless steel, and even Cu surfaces for 4-hours after application. While breathable N95-rated masks can capture particulates before they can be inhaled, SARS-CoV-2 virus particles remain active in mask filters for up to 7 days. Contact killing of viruses, such as observed on Cu surfaces is, therefore, receiving renewed interest as a disease mitigation strategy.
and increased contact time with copper and brass surfaces led to greater non-specific fragmentation of viral RNA, indicating irreversible viral inactivation. More recently, Doremalen et al. showed surface stability of both SARS-CoV-1 and SARS-CoV-2 virus on plastic, cardboard, stainless steel, and even Cu surfaces for 4-hours after application. While breathable N95-rated masks can capture particulates before they can be inhaled, SARS-CoV-2 virus particles remain active in mask filters for up to 7 days. Contact killing of viruses, such as observed on Cu surfaces is, therefore, receiving renewed interest as a disease mitigation strategy.
[0035] Surprisingly, compounds capable of endogenous nitrogen-release, such as Si3N4 and AIN, can inactivate the SARS-CoV-2 virus at least as effectively as Cu. Without being limited to any one theory, multiple antiviral mechanisms may be operative, such as RNA fragmentation, and in the case of Cu and AIN, direct metal ion toxicity; but while Cu and AIN supernatants demonstrated cellular lysis, Si3N4 may provoke no metabolic alterations. The Raman spectrum of VeroE6 cells exposed to the Si3N4 viral supernatant was like that of the uninfected sham. These findings indicate that while Si3N4, Cu, and AIN were all capable of inactivating the SARS-CoV-2 virus, Si3N4 was the safest.
[0036] The antiviral effect may be related to the electrical attraction (including "competitive binding" to an envelope glycoprotein hemagglutinin in the case of influenza virus) and viral RNA fragmentation by reactive nitrogen species (RNS). These phenomena are due to the slow and controlled elution of nitrogen from Si31\14's surface which forms ammonia (NH3) and ammonium (NH4) moieties coupled with the release of free electrons and negatively charged silanols in aqueous solution.
[0037] In the context of SARS-CoV-2 viral inactivation, two important aspects of Si3N4's surface chemistry play fundamental roles: (i) the similarity between the protonated amino groups, Si¨NH3+ at the surface of Si3N4 and the N-terminal of lysine, C¨NH3+ on the virus; and, (ii) the elution of gaseous ammonia due to Si3N4 hydrolysis. A schematic representation of the interaction between SARS-CoV-2 and the Si3N4 surface is given in FIG. 6 (central panel). The similarity is depicted in the left panel of this figure. It triggers an extremely effective "competitive binding" approach to SARS-CoV-2 inactivation which stems from several successful other examples such as Hepatitis B and Influenza A. The strong antiviral effect of eluted (gaseous) NH3 is due to its penetration of the virions and its reaction with the RNA backbone. The RNA undergoes alkaline transesterification through the hydrolysis of its phosphodiester bonds. RNA phosphodiester bond cleavage is schematically depicted in the right panel of FIG. 6. The RT-PCR and fluorescence microscopy results of the present study suggest contributions from both mechanisms to the inactivation of SARS-CoV-2, consistent with earlier work. The TCID5o results shown in FIGS. 1A-1D and the RT-PCR data of FIGS. 2A-2D for viral RNA
harvested from either the supernatant or the Si3N4 particles provide important information about these mechanisms. Although >99% inactivation was achieved after exposure to Si3N4 for 1-min, (FIG 1B), only partial viral RNA fragmentation was observed for the supernatant (FIG. 2A) while RNA harvested from the Si3N4 particles (FIG. 2B) was essentially fully fragmented. Note that the opposite effect was found for Cu.
This suggests that the mechanism of inactivation for Si3N4, as depicted in the left panel of FIG. 6, had successive events of "competitive binding" and ammonia poisoning ¨
a kind of "catch and kill" scenario. The complete RNA fragmentation at 10-minute exposure to Si3N4 suggests that nitrogen elution is a key process that triggers a cascade of reactions which result in virus inactivation (cf. right panel in FIG. 6)
harvested from either the supernatant or the Si3N4 particles provide important information about these mechanisms. Although >99% inactivation was achieved after exposure to Si3N4 for 1-min, (FIG 1B), only partial viral RNA fragmentation was observed for the supernatant (FIG. 2A) while RNA harvested from the Si3N4 particles (FIG. 2B) was essentially fully fragmented. Note that the opposite effect was found for Cu.
This suggests that the mechanism of inactivation for Si3N4, as depicted in the left panel of FIG. 6, had successive events of "competitive binding" and ammonia poisoning ¨
a kind of "catch and kill" scenario. The complete RNA fragmentation at 10-minute exposure to Si3N4 suggests that nitrogen elution is a key process that triggers a cascade of reactions which result in virus inactivation (cf. right panel in FIG. 6)
[0038] In some embodiments, an object, article, or composition comprising silicon nitride or aluminum nitride may be operable to successively bind a virus (e.g. SARS-Cov-2) and then inactivate the virus.
[0039] The antiviral effectiveness of Si3N4 may be comparable to Cu.
While Cu is an essential trace element for human health and an electron donor/acceptor for several key enzymes by altering redox states between Cu +
and Cu2+, these properties can also cause cellular damage. Its use as an antiviral agent is limited by allergic dermatitis, hypersensitivity, and multi-organ dysfunction. In contrast, the safety of Si3N4 as a permanently-implanted material during spine fusion surgery is well established by experimental and clinical data. Therefore, an object, article, or composition comprising silicon nitride may be as effective at inactivating a virus as Cu without the negative effects of Cu.
While Cu is an essential trace element for human health and an electron donor/acceptor for several key enzymes by altering redox states between Cu +
and Cu2+, these properties can also cause cellular damage. Its use as an antiviral agent is limited by allergic dermatitis, hypersensitivity, and multi-organ dysfunction. In contrast, the safety of Si3N4 as a permanently-implanted material during spine fusion surgery is well established by experimental and clinical data. Therefore, an object, article, or composition comprising silicon nitride may be as effective at inactivating a virus as Cu without the negative effects of Cu.
[0040] Si3N4 is well-known for its capabilities as an industrial material.
Load-bearing Si3N4 prosthetic hip bearings and spinal fusion implants were initially developed because of the superior strength and toughness of Si3N4. Later studies showed other properties of Si3N4 that are favored in designing orthopaedic implants, such as enhanced osteoconductivity, bacteriostasis, improved radiolucency, lack of implant subsidence, and wear resistance. Therefore, Si3N4's surface chemistry, topography, and hydrophilicity contribute to a dual effect (i.e., upregulation of osteogenic activity to promote spinal fusion while simultaneously preventing bacterial adhesion and biofilm formation). In addition to its proven record as a bioimplant, an advantage of Si3N4 is its versatility of manufacture. Sintered powders of Si3N4 have been incorporated into other materials, such as polymers, other ceramics, bioglass, and metals, to create composite structures that maintain the index osteogenic and antibacterial properties of monolithic Si3N4. Three-dimensional additive deposition of Si3N4 may enable the manufacture of protective surfaces in health care that reduce fomite-mediated transmission of microbial disease. Incorporation of Si3N4 particles into the fabric of personal protective equipment, such as face masks, protective gowns, and surgical drapes could contribute to health workers as well as patient safety.
Load-bearing Si3N4 prosthetic hip bearings and spinal fusion implants were initially developed because of the superior strength and toughness of Si3N4. Later studies showed other properties of Si3N4 that are favored in designing orthopaedic implants, such as enhanced osteoconductivity, bacteriostasis, improved radiolucency, lack of implant subsidence, and wear resistance. Therefore, Si3N4's surface chemistry, topography, and hydrophilicity contribute to a dual effect (i.e., upregulation of osteogenic activity to promote spinal fusion while simultaneously preventing bacterial adhesion and biofilm formation). In addition to its proven record as a bioimplant, an advantage of Si3N4 is its versatility of manufacture. Sintered powders of Si3N4 have been incorporated into other materials, such as polymers, other ceramics, bioglass, and metals, to create composite structures that maintain the index osteogenic and antibacterial properties of monolithic Si3N4. Three-dimensional additive deposition of Si3N4 may enable the manufacture of protective surfaces in health care that reduce fomite-mediated transmission of microbial disease. Incorporation of Si3N4 particles into the fabric of personal protective equipment, such as face masks, protective gowns, and surgical drapes could contribute to health workers as well as patient safety.
[0041] Si3N4 inactivates the SARS-CoV-2 virus in a matter of minutes following exposure. Without being limited to any one theory, the mechanism of action may be shared with other nitrogen-based compounds that express trace amounts of surface disinfectants, such as aluminum nitride.
[0042] In some embodiments, the object used to bind and inactivate the SARS-CoV-2 virus is a device or apparatus that may include a silicon nitride and/or aluminum nitride composition on at least a portion of a surface of the object.
The silicon nitride or aluminum nitride coating may be applied to the surface of the object as a powder. In some examples, the silicon nitride or aluminum nitride powder may be filled, embedded, or impregnated in at least a portion of the object. In some embodiments, the powder may have particles in the micron, submicron or nanometer size range. The average particle size may range from about 100 nm to about 5 pm, from about 300 nm to about 1.5 pm, or from about 0.6 pm to about 1.0 pm. In other embodiments, the silicon nitride or aluminum nitride may be incorporated into the device. For example, an object may incorporate a silicon nitride and/or aluminum nitride powder within the body of the object. In one embodiment, the device may be made of silicon nitride. In another embodiment, the object may be made of aluminum nitride. In yet another embodiment, the object can comprise a slurry or suspension of aluminum nitride or silicon nitride particles.
The silicon nitride or aluminum nitride coating may be applied to the surface of the object as a powder. In some examples, the silicon nitride or aluminum nitride powder may be filled, embedded, or impregnated in at least a portion of the object. In some embodiments, the powder may have particles in the micron, submicron or nanometer size range. The average particle size may range from about 100 nm to about 5 pm, from about 300 nm to about 1.5 pm, or from about 0.6 pm to about 1.0 pm. In other embodiments, the silicon nitride or aluminum nitride may be incorporated into the device. For example, an object may incorporate a silicon nitride and/or aluminum nitride powder within the body of the object. In one embodiment, the device may be made of silicon nitride. In another embodiment, the object may be made of aluminum nitride. In yet another embodiment, the object can comprise a slurry or suspension of aluminum nitride or silicon nitride particles.
[0043] In some embodiments, the object may further comprise other materials including, but not limited to, paper, cardboard, fabric, plastic, ceramic, polymers, stainless steel, metal, or a combination thereof. Some non-limiting examples of the object may include surgical gowns, surgical drapes, shoe covers, cubicle curtains, tubing, clothing, gloves, eye protectors, masks including surgical masks and face shields, PPE, tables such as hospital exam and surgical tables, chairs, bed frames, bed trays, desks, fixtures, cabinets, equipment racks, carts, handles, knobs, railings, toys, water filters, and air filters such as face mask filters, respirator filters, air filtration filters, and air ventilation filters, or air conditioner filters.
In some examples, the filters may be within filtration devices of anesthesia machines, ventilators, or CPAP machines such that an antimicrobial surface layer in the filter can trap pulmonary pathogens, as air moves in and out of infected lungs. In various embodiments, the object may be a medical device or apparatus. Non-limiting examples of medical devices or apparatuses include orthopedic implants, spinal implants, pedicle screws, dental implants, in-dwelling catheters, endotracheal tubes, colonoscopy scopes, and other similar devices.
In some examples, the filters may be within filtration devices of anesthesia machines, ventilators, or CPAP machines such that an antimicrobial surface layer in the filter can trap pulmonary pathogens, as air moves in and out of infected lungs. In various embodiments, the object may be a medical device or apparatus. Non-limiting examples of medical devices or apparatuses include orthopedic implants, spinal implants, pedicle screws, dental implants, in-dwelling catheters, endotracheal tubes, colonoscopy scopes, and other similar devices.
[0044] In other embodiments, the object may be a composition incorporating silicon nitride or aluminum nitride powder therein including, but not limited to slurries, suspensions, gels, sprays, paint, or toothpaste. For example, the addition of silicon nitride or aluminum nitride to a slurry, such as paint, that is then applied to a surface may provide an antibacterial, antifungal, and antiviral surface. In other embodiments, silicon nitride or aluminum nitride may be mixed with water along with any appropriate dispersants and slurry stabilization agents, and thereafter applied by spraying the slurry onto various surfaces. An example dispersant is Dolapix A88.
[0045] The silicon nitride or aluminum nitride coating may be present on the surface of the object in a concentration of about 1 wt.% to about 100 wt.%.
The silicon nitride and/or aluminum nitride may be coated onto or layered into the object. In various embodiments, the coating may include about 1 wt.%, 2 wt.%, wt.%, 7.5 wt.%, 8.3 wt. 70, 10 wt.%, 15 wt. 70, 16.7 wt.%, 20 wt. /0, 25 wt.
70, or about 30 wt.% silicon nitride powder or aluminum nitride powder. In some examples, the coating may include about 10 wt.% to about 20 wt.% silicon nitride or aluminum nitride. In at least one example, the coating includes about 15 wt.% silicon nitride or aluminum nitride. In some embodiments, silicon nitride or aluminum nitride may be embedded in (as a filler) or on the surface of the object in a concentration of about 1 wt.% to about 100 wt.%. In various embodiments, the object may include about 1 w1.%, 2 wt.%, 5 wt.%, 7.5 wt.%, 8.3 wt.%, 10 wt.%, 15 wt.%, 16.7 wt.%, 20 wt.%, 25 wt.%, 30 wt.%, 33.3 wt.%, 35 wt.%, 40 wt.%, 50 wt.%, 60 wt.%, 70 wt. %, 80 wt.%, 90 wt.%, to 100 wt.% silicon nitride or aluminum nitride. In some examples, the silicon nitride or aluminum nitride may be on the surface of the object at a concentration of about 10 wt.% to about 20 wt.%. In at least one example, the silicon nitride or aluminum nitride may be on the surface of the object at a concentration of about 15 wt.%. In some aspects, the concentration of silicon nitride or aluminum nitride may depend on the substrate material of the object, such as paper, cardboard, fabric, plastic, ceramic, polymers, stainless steel, and/or metal.
In some embodiments, the substrate material of the object may be a polymer and the polymer may have a practical limit (i.e. percolation limit) on the amount of silicon nitride and/or aluminum nitride that may be incorporated into the object.
The silicon nitride and/or aluminum nitride may be coated onto or layered into the object. In various embodiments, the coating may include about 1 wt.%, 2 wt.%, wt.%, 7.5 wt.%, 8.3 wt. 70, 10 wt.%, 15 wt. 70, 16.7 wt.%, 20 wt. /0, 25 wt.
70, or about 30 wt.% silicon nitride powder or aluminum nitride powder. In some examples, the coating may include about 10 wt.% to about 20 wt.% silicon nitride or aluminum nitride. In at least one example, the coating includes about 15 wt.% silicon nitride or aluminum nitride. In some embodiments, silicon nitride or aluminum nitride may be embedded in (as a filler) or on the surface of the object in a concentration of about 1 wt.% to about 100 wt.%. In various embodiments, the object may include about 1 w1.%, 2 wt.%, 5 wt.%, 7.5 wt.%, 8.3 wt.%, 10 wt.%, 15 wt.%, 16.7 wt.%, 20 wt.%, 25 wt.%, 30 wt.%, 33.3 wt.%, 35 wt.%, 40 wt.%, 50 wt.%, 60 wt.%, 70 wt. %, 80 wt.%, 90 wt.%, to 100 wt.% silicon nitride or aluminum nitride. In some examples, the silicon nitride or aluminum nitride may be on the surface of the object at a concentration of about 10 wt.% to about 20 wt.%. In at least one example, the silicon nitride or aluminum nitride may be on the surface of the object at a concentration of about 15 wt.%. In some aspects, the concentration of silicon nitride or aluminum nitride may depend on the substrate material of the object, such as paper, cardboard, fabric, plastic, ceramic, polymers, stainless steel, and/or metal.
In some embodiments, the substrate material of the object may be a polymer and the polymer may have a practical limit (i.e. percolation limit) on the amount of silicon nitride and/or aluminum nitride that may be incorporated into the object.
[0046] In some embodiments, the object may be a monolithic component consisting of the silicon nitride or aluminum nitride. Such an object may be fully dense possessing no internal porosity, or it may be porous, having a porosity that ranges from about 1% to about 80%. The monolithic object may be used as a medical device or may be used in an apparatus in which the inactivation of a virus may be desired.
[0047] In some embodiments, the object may contact the SARS-CoV-2 virus for a limited period of time. The object may be in contact with the SARS-CoV-2 virus for about 1 min to about 2 hours in order to inactivate the virus. In various examples, the object may contact the SARS-CoV-2 virus for at least 30 seconds, at least 1 minute, at least 5 minutes, at least 30 minutes, at least 1 hour, at least 2 hours, at least 5 hours, or at least 1 day. In at least one example, the object may be permanently implanted in the patient. In at least one example, the object may be worn externally by a user. In another example, the object may be permanently implanted in the patient. In yet another example, the object may be a high contact surface. In further examples, the object may be in continuous or sustained contact with a body fluid of a patient. The body fluid may be blood or gas (e.g., inhalation or exhalation gas).
[0048] In some embodiments, the virus is at least 70%
inactivated, at least 75% inactivated, at least 80% inactivated, at least 85% inactivated, at least 90% inactivated, at least 95% inactivated, or at least 99% inactivated after contact with the object for at least 1 minute, at least 5 minutes, or at least 30 minutes. In at least one example, the virus is at least 85% inactivated after contact with object for at least 1 minute. In another example, the virus is at least 99% inactivated after contact with the object for at least 30 minutes. In yet another example, the virus is at least 99% inactivated after contact with the object for at least 1 minute.
inactivated, at least 75% inactivated, at least 80% inactivated, at least 85% inactivated, at least 90% inactivated, at least 95% inactivated, or at least 99% inactivated after contact with the object for at least 1 minute, at least 5 minutes, or at least 30 minutes. In at least one example, the virus is at least 85% inactivated after contact with object for at least 1 minute. In another example, the virus is at least 99% inactivated after contact with the object for at least 30 minutes. In yet another example, the virus is at least 99% inactivated after contact with the object for at least 1 minute.
[0049] Also presented herein is an article of personal protective equipment having antiviral and antimicrobial properties. The article may comprise silicon nitride or aluminum nitride incorporated into the article or the silicon nitride or aluminum nitride may be coated onto the surface of the article.
[0050] The silicon nitride or aluminum nitride coating may be present on the surface of the article in a concentration of about 1 wt.% to about 100 wt.%. In various embodiments, the coating may include about 1 wt.%, 2 wt.%, 5 wt.%, 7.5 wt.%, 8.3 wt.%, 10 wt.%, 15 wt.%, 16.7 wt.%, 20 wt.%, 25 wt.%, or about 30 wt.%
silicon nitride powder or aluminum nitride powder. In some examples, the coating may include about 10 wt.% to about 20 wt.% silicon nitride or aluminum nitride. In at least one example, the coating includes about 15 wt.% silicon nitride or aluminum nitride. In some embodiments, silicon nitride or aluminum nitride may be embedded in (as a filler) or on the surface of the article in a concentration of about 1 wt.% to about 100 wt.%. In various embodiments, the object may include about 1 wt.%, 2 wt.%, 5 wt.%, 7.5 wt.%, 8.3 wt.%, 10 wt.%, 15 wt.%, 16.7 wt.%, 20 wt.%, 25 wt.%, 30 wt.%, 33.3 wt.%, 35 wt.%, 40 wt.%, 50 wt.%, 60 wt.%, 70 wt. %, 80 wt.%, 90 wt.%, to 100 wt.% silicon nitride or aluminum nitride. In some examples, the silicon nitride or aluminum nitride may be on the surface of the article at a concentration of about 10 wt.% to about 20 wt.%. In at least one example, the silicon nitride or aluminum nitride may be on the surface of the article at a concentration of about 15 wt.%. In some aspects, the concentration of silicon nitride or aluminum nitride may depend on the substrate material of the object.
silicon nitride powder or aluminum nitride powder. In some examples, the coating may include about 10 wt.% to about 20 wt.% silicon nitride or aluminum nitride. In at least one example, the coating includes about 15 wt.% silicon nitride or aluminum nitride. In some embodiments, silicon nitride or aluminum nitride may be embedded in (as a filler) or on the surface of the article in a concentration of about 1 wt.% to about 100 wt.%. In various embodiments, the object may include about 1 wt.%, 2 wt.%, 5 wt.%, 7.5 wt.%, 8.3 wt.%, 10 wt.%, 15 wt.%, 16.7 wt.%, 20 wt.%, 25 wt.%, 30 wt.%, 33.3 wt.%, 35 wt.%, 40 wt.%, 50 wt.%, 60 wt.%, 70 wt. %, 80 wt.%, 90 wt.%, to 100 wt.% silicon nitride or aluminum nitride. In some examples, the silicon nitride or aluminum nitride may be on the surface of the article at a concentration of about 10 wt.% to about 20 wt.%. In at least one example, the silicon nitride or aluminum nitride may be on the surface of the article at a concentration of about 15 wt.%. In some aspects, the concentration of silicon nitride or aluminum nitride may depend on the substrate material of the object.
[0051] In some embodiments, the article is PPE. In some aspects, the article is a body cover, a head cover, a shoe cover, a face mask, a face and eye protector, or gloves. In some aspects, the article is operable to inactivate a SARS-CoV-2 virus when the article contacts the virus.
Examples Example 1:
Preparation of Test Materials
Examples Example 1:
Preparation of Test Materials
[0052] Si3N4, Cu, and AIN powders were acquired from commercial sources. Si3N4 powder (nominal composition of 90 wt.% Si3N4, 6 wt.% Y203, and wt.% A1203) was prepared by aqueous mixing and spray-drying of the inorganic constituents, followed by sintering of the spray-dried granules (-1700 C for -3 h), hot-isostatic pressing (-1600 C, 2 h, 140 MPa in N2), aqueous-based comminution, and freeze-drying. The resulting powder had an average particle size of 0.8 1.0 pm. As-received Cu powder (USP grade 99.5% purity) granules were comminuted to achieve a particle size comparable to the Si3N4. AIN powder had an average particle size of 1.2 0.6 pm as-received, which was comparable to Si3N4.
Preparation of Mammalian and Viral Cells
Preparation of Mammalian and Viral Cells
[0053] VeroE6/TMPRSS2 mammalian cells were used in the viral assays. Cells were grown in Dulbecco's modified Eagle's minimum essential medium (DMEM) supplemented with G418 disulfate (1 mg/ml), penicillin (100 units/mL), streptomycin (100 pg/mL), 5% fetal bovine serum, and maintained at 37 C in a 5%
CO2 / 95% in a humidified atmosphere. The SARS-CoV-2 viral stock was propagated using VeroE6/TMPRSS2 cells at 37 C for 2 days. Viral titers were assayed by a median tissue culture infectious dose (TCIDso).
Example 2:
Virus Assays
CO2 / 95% in a humidified atmosphere. The SARS-CoV-2 viral stock was propagated using VeroE6/TMPRSS2 cells at 37 C for 2 days. Viral titers were assayed by a median tissue culture infectious dose (TCIDso).
Example 2:
Virus Assays
[0054] Fifteen weight percent (15 wt.%) of the Si3N4, Cu, and AIN
powders were separately dispersed in 1 mL of PBS(-), followed by the addition of the viral suspension (2 x 105 TO! Ds in 20 pL). Due to the higher density of the Cu powder, its volumetric fraction was approximately one-third of the Si3N4.
Mixing was performed for 1 and 10 minutes by slow manual rotation at 4 C. After exposure, the powders were pelleted by centrifugation (2400 rpm 2 minutes) followed by filtration through a 0.1 pm media. Supernatants were collected and subjected to TO! Ds assays, real-time RT-PCR testing, and fluorescence imaging. Experiments were performed in triplicate including sham supernatants without the antiviral powders. A
confluent nnonolayer of VeroE6/TMPRSS2 cells in a 96-well plate was inoculated with 50 pL/well of each virus suspension in a tenfold serial dilution with 0.5% FBS
DMEM (i.e., maintenance medium). Viral adsorption at 37 C for 1 hour was made with tilting every 10 minutes. Afterwards, 50 pL/well of the maintenance medium was added. The plate was incubated at 37 C in a 5% CO2 / 95% humidified atmosphere for 4 days. The cytopathic effect (CPE) of the infected cells was observed under a phase-contrast microscope. The cells were subsequently fixed by adding 10 pL/well of glutaraldehyde followed by staining with 0.5% crystal violet. The TO! Dso was calculated according to the Reed-Muench method.
Viral RNA Assay
powders were separately dispersed in 1 mL of PBS(-), followed by the addition of the viral suspension (2 x 105 TO! Ds in 20 pL). Due to the higher density of the Cu powder, its volumetric fraction was approximately one-third of the Si3N4.
Mixing was performed for 1 and 10 minutes by slow manual rotation at 4 C. After exposure, the powders were pelleted by centrifugation (2400 rpm 2 minutes) followed by filtration through a 0.1 pm media. Supernatants were collected and subjected to TO! Ds assays, real-time RT-PCR testing, and fluorescence imaging. Experiments were performed in triplicate including sham supernatants without the antiviral powders. A
confluent nnonolayer of VeroE6/TMPRSS2 cells in a 96-well plate was inoculated with 50 pL/well of each virus suspension in a tenfold serial dilution with 0.5% FBS
DMEM (i.e., maintenance medium). Viral adsorption at 37 C for 1 hour was made with tilting every 10 minutes. Afterwards, 50 pL/well of the maintenance medium was added. The plate was incubated at 37 C in a 5% CO2 / 95% humidified atmosphere for 4 days. The cytopathic effect (CPE) of the infected cells was observed under a phase-contrast microscope. The cells were subsequently fixed by adding 10 pL/well of glutaraldehyde followed by staining with 0.5% crystal violet. The TO! Dso was calculated according to the Reed-Muench method.
Viral RNA Assay
[0055] After exposure to the powders, 140 pL of the supernatants were used for viral RNA extraction. RNA was also extracted from the surfaces of the centrifuged and filtered powders. RNA purification was performed by using a QIAamp Viral RNA Mini kit. An aliquot of 16 pL of isolated RNA was reverse-transcribed using ReverTra Ace qPCR RT Master Mix. Quantitative real-time PCR
was performed using a Step-One Plus Real-Time PCR system primers/probes for two specific viral N gene sets. Each 20 pL reaction mixture contained 4 pL of cDNA, 8.8 pmol of each primer, 2.4 pmol of the probe, and 10 pL GoTaq Probe qPCR
master mix. The amplification protocol consisted of 50 cycles of denaturation at 95 C
for 3 seconds and annealing and extension at 60 C for 20 seconds.
lmmunochemical Fluorescence Assay
was performed using a Step-One Plus Real-Time PCR system primers/probes for two specific viral N gene sets. Each 20 pL reaction mixture contained 4 pL of cDNA, 8.8 pmol of each primer, 2.4 pmol of the probe, and 10 pL GoTaq Probe qPCR
master mix. The amplification protocol consisted of 50 cycles of denaturation at 95 C
for 3 seconds and annealing and extension at 60 C for 20 seconds.
lmmunochemical Fluorescence Assay
[0056] Vero E6/TMPRSS2 cells on cover glass were inoculated with 200 pL of virus supernatant. After viral adsorption at 37 C for 1 hour, the cells were incubated with the maintenance medium in a CO2 incubator for 7 hour. For the detection of infected cells, these cells were washed with TBS (20 mM Tris-HCI
pH
7.5, 150 mM NaCI) and fixed with 4% PFA for 10 min at room temperature (RT) followed by membrane permeabilization with 0.1% Triton X in TBS for 5 minutes at RT. The cells were blocked with 2% skim milk in TBS for 60 minutes at RT and stained with anti-SARS Coronavirus envelope (Rabbit) antibody (Dilution =1:100) for 60 minutes at RT. After washing with a buffer, cells were incubated with an Alexa 594 Goat Anti-Rabbit IgG (H+L) (1:500) and Alexa 488 Phalloidin (1:50) for 60 min at RT
in the dark. ProLong TM Diamond Antifade Mountant with DAPI was used as a mounting medium. The staining was observed under a fluorescent microscope BZX710. The total cell and infected cell counts were obtained using the Keyence BZ-X Analyzer.
Raman Spectroscopy Assay
pH
7.5, 150 mM NaCI) and fixed with 4% PFA for 10 min at room temperature (RT) followed by membrane permeabilization with 0.1% Triton X in TBS for 5 minutes at RT. The cells were blocked with 2% skim milk in TBS for 60 minutes at RT and stained with anti-SARS Coronavirus envelope (Rabbit) antibody (Dilution =1:100) for 60 minutes at RT. After washing with a buffer, cells were incubated with an Alexa 594 Goat Anti-Rabbit IgG (H+L) (1:500) and Alexa 488 Phalloidin (1:50) for 60 min at RT
in the dark. ProLong TM Diamond Antifade Mountant with DAPI was used as a mounting medium. The staining was observed under a fluorescent microscope BZX710. The total cell and infected cell counts were obtained using the Keyence BZ-X Analyzer.
Raman Spectroscopy Assay
[0057] Vero E6/TMPRSS2 cells were infected with 200 pL of each virus suspension onto glass sites. After viral adsorption at 37 C for 1 hours, the infected cells were incubated with the maintenance medium in a CO2 incubator for 4 hours and fixed with 4% paraformaldehyde for 10 minutes at RT. After washing with distilled water twice, infected cells were air-dried and in situ analyzed using a Raman microprobe spectrometer. Raman spectra were collected using a highly sensitive spectroscope with a 20x optical lens. It operated in microscopic measurement mode with confocal imaging in two dimensions. A holographic notch filter within the optical circuit was used to efficiently achieve a spectral resolution of 1.5 cm-1 via a 532 nm excitation source operating at 10 mW. Raman emissions were monitored using a single monochromator connected to an air-cooled charge-coupled device (CCD) detector 1024 x 256 pixels). The acquisition time was fixed at 10 seconds.
Thirty spectra were collected and averaged for each analysis time-point. Raman spectra were deconvoluted into Gaussian¨Lorentzian sub-bands using commercially available software.
Statistical Analysis
Thirty spectra were collected and averaged for each analysis time-point. Raman spectra were deconvoluted into Gaussian¨Lorentzian sub-bands using commercially available software.
Statistical Analysis
[0058] The Student's t-test determined statistical significance for n=3 and at a p-value of 0.01 using Prism software.
Example 3:
Median Tissue Culture Infection Dose
Example 3:
Median Tissue Culture Infection Dose
[0059] TO! Dso assay results for the 15 wt.% Si3N4, Cu, and AIN
powders are shown in FIGS. 1A-1D. Inactivation times of 1 and 10 minutes are shown in FIGS. 1A and 1B as well as FIGS. 1C and 1D, respectively. Relative to the negative control, all three powders were effective in inactivating SARS-CoV-2 virions (>99%) for the two exposure times.
RNA Gene Fragmentation
powders are shown in FIGS. 1A-1D. Inactivation times of 1 and 10 minutes are shown in FIGS. 1A and 1B as well as FIGS. 1C and 1D, respectively. Relative to the negative control, all three powders were effective in inactivating SARS-CoV-2 virions (>99%) for the two exposure times.
RNA Gene Fragmentation
[0060] To examine whether viral RNA was fragmented from exposure to both the supernatants and powders, RT-PCR tests were conducted on the N gene sets of the virus' RNA. The results are shown in Fig. 2A and 2B as well as FIGS. 2C
and 2D for 1- and 10-minute exposures, respectively. Again, in comparison to the negative control at 1 minute of exposure to the supernatants, almost complete fragmentation of the RNA was observed for Cu while significant damage was caused by AIN and to a lesser extent by Si3N4. After 10-minute exposure to the supernatants, substantial cleavage of the RNA was seen for all three materials. While Cu still showed the most fragmentation, Si31\14 demonstrated similar effectiveness, and AIN
was essentially identical to the 1-minute exposure condition. Viral RNA was virtually undetectable for all three materials based on extracted RNA from the pelleted powders at 1-minute of exposure (cf., FIGS. 2A and 2B). This result suggests that the decrease of viral RNA in the supernatant was not because of adherence of the RNA to the powders, but rather due to direct degradation.
lmmunofluorescence Testing
and 2D for 1- and 10-minute exposures, respectively. Again, in comparison to the negative control at 1 minute of exposure to the supernatants, almost complete fragmentation of the RNA was observed for Cu while significant damage was caused by AIN and to a lesser extent by Si3N4. After 10-minute exposure to the supernatants, substantial cleavage of the RNA was seen for all three materials. While Cu still showed the most fragmentation, Si31\14 demonstrated similar effectiveness, and AIN
was essentially identical to the 1-minute exposure condition. Viral RNA was virtually undetectable for all three materials based on extracted RNA from the pelleted powders at 1-minute of exposure (cf., FIGS. 2A and 2B). This result suggests that the decrease of viral RNA in the supernatant was not because of adherence of the RNA to the powders, but rather due to direct degradation.
lmmunofluorescence Testing
[0061] Immunofluorescence imaging using anti-SARS
coronavirus envelope antibody (red), Phalloidin that stains F-actin in viable cells (green), and DAPI for cell nuclear staining (blue) was then used to confirm the TCID50 assay and gene fragmentation results. FIGS. 3A-3D show fluorescence micrographs representative of the VeroE6/TMPRSS2 cell populations that were inoculated with supernatants of (a) unexposed virions (i.e., negative control) and 10-minute-exposed virions of (b) Si31\14, (c) AIN, and (d) Cu. FIG. 3E shows cells that were not inoculated with the virus (labeled as "sham-infected" cells. The red-colored spots in the negative control (FIG. 3A) demonstrated that the virions had entered and hijacked the Vero6E
cells' metabolism. This contrasts with the sham-infected cells (FIG. 3E) which showed normal metabolic function.
coronavirus envelope antibody (red), Phalloidin that stains F-actin in viable cells (green), and DAPI for cell nuclear staining (blue) was then used to confirm the TCID50 assay and gene fragmentation results. FIGS. 3A-3D show fluorescence micrographs representative of the VeroE6/TMPRSS2 cell populations that were inoculated with supernatants of (a) unexposed virions (i.e., negative control) and 10-minute-exposed virions of (b) Si31\14, (c) AIN, and (d) Cu. FIG. 3E shows cells that were not inoculated with the virus (labeled as "sham-infected" cells. The red-colored spots in the negative control (FIG. 3A) demonstrated that the virions had entered and hijacked the Vero6E
cells' metabolism. This contrasts with the sham-infected cells (FIG. 3E) which showed normal metabolic function.
[0062] Remarkably, cells inoculated with supernatants from Si3N4 and, to a lesser extent, from AIN demonstrated near-normal function with few infections.
Conversely, cells inoculated with the Cu supernatant were essentially dead (i.e., a complete lack of F-actin, FIG. 3D), although based on the bluish-red stains present in the nuclei, they may have been viable premortem because virions appear to have hijacked some nuclei. This suggests that cellular lysis was not only the result of the viral infection but also due to toxic effects from intracellular free Cu ions.
Quantification of the colorimetric results from FIGS. 3A-3E is provided in FIG. 4.
These data demonstrate that about 35% of the viable VeroE6 cells from the negative control were infected by virions, whereas only 2% and 8% of cells inoculated with supernatants from Si3N4 and AIN were infected, respectively. Quantitative evaluation of the cells inoculated with the Cu supernatant could not be assessed due to their premature death.
Raman Spectroscopy
Conversely, cells inoculated with the Cu supernatant were essentially dead (i.e., a complete lack of F-actin, FIG. 3D), although based on the bluish-red stains present in the nuclei, they may have been viable premortem because virions appear to have hijacked some nuclei. This suggests that cellular lysis was not only the result of the viral infection but also due to toxic effects from intracellular free Cu ions.
Quantification of the colorimetric results from FIGS. 3A-3E is provided in FIG. 4.
These data demonstrate that about 35% of the viable VeroE6 cells from the negative control were infected by virions, whereas only 2% and 8% of cells inoculated with supernatants from Si3N4 and AIN were infected, respectively. Quantitative evaluation of the cells inoculated with the Cu supernatant could not be assessed due to their premature death.
Raman Spectroscopy
[0063] Raman spectroscopy examined VeroE6 cells exposed to the various supernatants to assess biochemical cellular changes due to infection and ionic (i.e., Cu and Al) toxicity. FIGS. 5A-5G show Raman spectra in the frequency range 700-900 cm-1 for (a) uninfected VeroE6/TMPRSS2 cells, and cells inoculated with supernatants containing virions exposed for 10-minutes to (b) Si3N4, (c) AIN, (d) Cu (positive control), and (e) no antiviral compounds (negative control). Of fundamental importance are the vibrational bands of ring breathing and H-scissoring of the indole ring of tryptophan (at 756 and 875 cm-1, labeled as Ti and T2, respectively). Tryptophan plays a vital role in protein synthesis and the generation of molecules for various immunological functions. Its stereoisomers serve to anchor proteins within the cell membrane and its catabolites possess immunosuppressive functions. The catabolism of tryptophan is triggered by a viral infection.
This occurs via the enzymatic activity of indoleamine-2,3-dioxygenase (IDO) which protects the host cells from an over-reactive immune response. IDO reduces tryptophan to kynurenine and then to N'-formyl-kynurenine. An increase in IDO activity depletes tryptophan. Consequently, the intensity of the tryptophan bands (Ti and T2) is an indicator of these biochemical changes. Except for the Cu-treated sample, the data presented in FIG. 5F show an exponential decline in the combined tryptophan bands that correlates with the fraction of infected cells. (The chemical structure of N'-formyl-kynurenine is given in the inset for clarity.) The anomaly for copper provides further evidence of its toxicity. The VeroE6 cells consumed tryptophan to reduce Cu2+
and stabilize it as Cu.
This occurs via the enzymatic activity of indoleamine-2,3-dioxygenase (IDO) which protects the host cells from an over-reactive immune response. IDO reduces tryptophan to kynurenine and then to N'-formyl-kynurenine. An increase in IDO activity depletes tryptophan. Consequently, the intensity of the tryptophan bands (Ti and T2) is an indicator of these biochemical changes. Except for the Cu-treated sample, the data presented in FIG. 5F show an exponential decline in the combined tryptophan bands that correlates with the fraction of infected cells. (The chemical structure of N'-formyl-kynurenine is given in the inset for clarity.) The anomaly for copper provides further evidence of its toxicity. The VeroE6 cells consumed tryptophan to reduce Cu2+
and stabilize it as Cu.
[0064] The Raman signals due to ring-stretching vibrations of adenine, cytosine, guanine, and thymine were found at 725, 795, 680, and 748 cm-1, and are labeled as A, Cy1, G, and Th, respectively, in FIGS. 5A-5E). These bands were preserved after virus exposure. However, there was an anomaly for lines representative of tyrosine at 642 and 832 cm-1 labeled as Ty1 and Ty2, respectively for cells infected with Cu-exposed virions. The ring-breathing band Ty2 of tyrosine was very weak compared to the other samples (cf. FIG. 5D with FIG. 5B).
Conversely, the C-C bond-related Ty1 signal remained strong. This suggests that the aromatic ring of tyrosine chelated the Cu ions. This explains why only the tyrosine ring-breathing mode was reduced while the C-C signal remained unaltered. Three possible Cu(II) chelating conformations in tyrosine are given in FIG. 5G.
Conversely, the C-C bond-related Ty1 signal remained strong. This suggests that the aromatic ring of tyrosine chelated the Cu ions. This explains why only the tyrosine ring-breathing mode was reduced while the C-C signal remained unaltered. Three possible Cu(II) chelating conformations in tyrosine are given in FIG. 5G.
[0065] For VeroE6 cells exposed to virions treated with AIN (FIG. 5C), the tryptophan Ti and T2 bands were preserved, but the bands at 615 and -700 cm-1 due to ring bending in DNA cytosine (labeled as Cy2 and Cy3, respectively, in FIGS. 5A-5E) almost vanished. Their disappearance is due to either progressive intemucleosomal DNA cleavage or from the formation of complexes, and both are related to toxicity. The loss of the cytosine signals is interpreted as a toxic effect by Al ions, although it is far less critical than copper. Al3+ interacts with carbonyl 0 and/or N
ring donors in nucleotide bases and selectively binds to the backbone of the group and/or to the guanine N-7 site of the G-C base pairs by chelation.
ring donors in nucleotide bases and selectively binds to the backbone of the group and/or to the guanine N-7 site of the G-C base pairs by chelation.
[0066] Unlike exposure of the VeroE6 cells to Cu and AIN
supernatants, which resulted in moderate to severe toxicity, Si3N4 invoked no modifications of tryptophan, tyrosine, and cytosine. The morphology of the spectrum for the Si3N4 viral supernatant closely matched that of the uninfected sham suspension (cf.
FIGS.
5A and 5B).
supernatants, which resulted in moderate to severe toxicity, Si3N4 invoked no modifications of tryptophan, tyrosine, and cytosine. The morphology of the spectrum for the Si3N4 viral supernatant closely matched that of the uninfected sham suspension (cf.
FIGS.
5A and 5B).
[0067] It should be understood from the foregoing that, while particular embodiments have been illustrated and described, various modifications can be made thereto without departing from the spirit and scope of the invention as will be apparent to those skilled in the art. Such changes and modifications are within the scope and teachings of this invention as defined in the claims appended hereto.
[0068] Having described several embodiments, it will be recognized by those skilled in the art that various modifications, alternative constructions, and equivalents may be used without departing from the spirit of the invention.
Additionally, a number of well-known processes and elements have not been described in order to avoid unnecessarily obscuring the present invention.
Accordingly, the above description should not be taken as limiting the scope of the invention.
Additionally, a number of well-known processes and elements have not been described in order to avoid unnecessarily obscuring the present invention.
Accordingly, the above description should not be taken as limiting the scope of the invention.
[0069] Those skilled in the art will appreciate that the presently disclosed embodiments teach by way of example and not by limitation.
Therefore, the matter contained in the above description or shown in the accompanying drawings should be interpreted as illustrative and not in a limiting sense.
The following claims are intended to cover all generic and specific features described herein, as well as all statements of the scope of the present method and system, which, as a matter of language, might be said to fall therebetween.
Therefore, the matter contained in the above description or shown in the accompanying drawings should be interpreted as illustrative and not in a limiting sense.
The following claims are intended to cover all generic and specific features described herein, as well as all statements of the scope of the present method and system, which, as a matter of language, might be said to fall therebetween.
Claims (17)
1. A method for inactivating SARS-Cov-2 virus, the method comprising contacting the SARS-Cov-2 virus with an object comprising silicon nitride and/or aluminum nitride, wherein the silicon nitride or aluminum nitride successively binds and inactivates the virus.
2. The method of claim 1, wherein the silicon nitride and/or aluminum nitride is incorporated into or is present as a coating on a surface of the object.
3. The method of claims 1 or 2, wherein the silicon nitride and/or aluminum nitride is present at a concentration from about 1 wt.% to about 30 wt.%.
4. The method of any one of claims 1 to 3, wherein the object further comprises paper, cardboard, fabric, plastic, ceramic, stainless steel, metal, or a combination thereof.
5. The method of any one of claims 1 to 4 wherein the object is a protective gown, a body cover, a head cover, a shoe cover, a face mask, a face shield, an eye protector, gloves, a surgical gown, a surgical drape, or a cubicle curtain.
6. The method of any one of claims 1 to 4, wherein the object is a face mask filter, a respirator filter, an air filtration filter, or an air ventilation filter.
7. The method of any one of claims 1 to 4, wherein the object is a knob, a handle, a railing, a bed frame, a bed tray, a table, a chair, an equipment rack, a cabinet, or a cart.
8. The method of any one of claims 1 to 7, wherein the object is in contact with the SARS-CoV-2 virus for at least one minute.
9. The method of any of claims 1-8, wherein at least 75% of the SARS-CoV-2 virus is inactivated after contact with the object.
10. The method of any of claims 1-9, wherein at least 99% of the SARS-CoV-2 virus is inactivated after contact with the object for at least 1 minute.
11. A method for inactivating SARS-Cov-2 virus, the method comprising contacting the SARS-Cov-2 virus with a composition comprising silicon nitride or aluminum nitride, wherein the silicon nitride and/or aluminum nitride successively binds and inactivates the virus.
12. The method of claim 11, wherein the composition comprises a slurry, suspension, gel, paint, or toothpaste.
13. The method of claim 11, wherein the composition is a slurry that is sprayed onto a high-contact surface.
14. An article of personal protective equipment (PPE) having antiviral and antimicrobial properties, the article comprising silicon nitride or aluminum nitride embedded in or coated onto a surface of the article, wherein the silicon nitride or aluminum nitride is operable to successively bind and inactivate the SARS-CoV-2 virus.
15. The article of claim 14, wherein the silicon nitride and/or aluminum nitride is present at a concentration from about 1 wt.% to about 30 wt.%.
16. The article of claim 14, which is a body cover, a head cover, a shoe cover, a face mask, a face and eye protector, or gloves.
17. The article of claim 14, wherein the article is operable to inactivate the SARS-CoV-2 virus when the article contacts the virus for at least 1 minute.
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US202063045355P | 2020-06-29 | 2020-06-29 | |
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PCT/US2021/027263 WO2022005550A1 (en) | 2020-06-29 | 2021-04-14 | Systems and methods for rapid inactivation of sars-cov-2 by silicon nitride and aluminum nitride |
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EP (1) | EP4171225A1 (en) |
JP (1) | JP2023532452A (en) |
KR (1) | KR20230029646A (en) |
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- 2021-04-14 BR BR112022026025A patent/BR112022026025A2/en not_active Application Discontinuation
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