CA3149221A1 - Procede de recherche d'organismes specifiques chez un individu - Google Patents

Procede de recherche d'organismes specifiques chez un individu Download PDF

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Publication number
CA3149221A1
CA3149221A1 CA3149221A CA3149221A CA3149221A1 CA 3149221 A1 CA3149221 A1 CA 3149221A1 CA 3149221 A CA3149221 A CA 3149221A CA 3149221 A CA3149221 A CA 3149221A CA 3149221 A1 CA3149221 A1 CA 3149221A1
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CA
Canada
Prior art keywords
individual
microbiome
patient
clostridium
stool sample
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Pending
Application number
CA3149221A
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English (en)
Inventor
Sabine HAZAN
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Individual
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Individual
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Publication date
Application filed by Individual filed Critical Individual
Publication of CA3149221A1 publication Critical patent/CA3149221A1/fr
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne un procédé de recherche d'organismes spécifiques chez un individu, lequel procédé comprend les étapes consistant à: a) dépister l'individu ; b) acquérir un échantillon de selles de l'individu; c) traiter l'échantillon de selles pour obtenir le microbiome de l'individu; d) séquencer le microbiome de l'individu; et e) analyser le microbiome de l'individu pour déterminer si un ou plusieurs organismes spécifiques sont présents chez l'individu, ce qui permet de déterminer un état de santé de l'individu. L'étape de traitement peut comprendre les sous-étapes suivantes i) l'extraction de l'ADN de l'échantillon de selles, laquelle comprend l'ajout de l'échantillon de selles dans un tube à billes d'agitation, la réalisation d'une lyse cellulaire, la capture de l'ADN sur une membrane de silice dans une colonne de centrifugation, et le lavage et l'élution de l'ADN à partir de la membrane ; et ii) la purification de l'ADN extrait. L'invention concerne également un procédé permettant de déterminer si un individu présente un état de santé comprenant les mêmes étapes. L'invention concerne également un kit de collecte d'échantillon de selles.
CA3149221A 2019-08-02 2020-07-31 Procede de recherche d'organismes specifiques chez un individu Pending CA3149221A1 (fr)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US201962882367P 2019-08-02 2019-08-02
US62/882,367 2019-08-02
US201962892409P 2019-08-27 2019-08-27
US62/892,409 2019-08-27
US202062991190P 2020-03-18 2020-03-18
US62/991,190 2020-03-18
US202063002486P 2020-03-31 2020-03-31
US63/002,486 2020-03-31
PCT/US2020/044605 WO2021026025A2 (fr) 2019-08-02 2020-07-31 Procédé de recherche d'organismes spécifiques chez un individu

Publications (1)

Publication Number Publication Date
CA3149221A1 true CA3149221A1 (fr) 2021-02-11

Family

ID=74503987

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3149221A Pending CA3149221A1 (fr) 2019-08-02 2020-07-31 Procede de recherche d'organismes specifiques chez un individu

Country Status (4)

Country Link
EP (1) EP3994283A4 (fr)
AU (1) AU2020326600A1 (fr)
CA (1) CA3149221A1 (fr)
WO (1) WO2021026025A2 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114369671A (zh) * 2021-11-25 2022-04-19 上海锐翌生物科技有限公司 风湿性关节炎标志微生物及其应用
CN114410809A (zh) * 2021-12-08 2022-04-29 上海金翌生物科技有限公司 慢传输便秘标志微生物及其应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009520957A (ja) * 2005-12-21 2009-05-28 エフ．ホフマン−ラ ロシュ アーゲー 便試料中のヘモグロビンおよびｍ２−ｐｋを測定することによる結腸直腸癌の評価方法
FR2945545B1 (fr) * 2009-05-14 2011-08-05 Univ Aix Marseille Ii Methode de detection d'adn procaryote extrait d'un echantillon de selles
EP2971127B1 (fr) * 2013-03-14 2018-02-28 University Of Ottawa Procédés de diagnostic et de traitement de maladie intestinale inflammatoire
WO2016164402A1 (fr) * 2015-04-06 2016-10-13 Oklahoma Medical Research Foundation Méthodes de diagnostic et de traitement d'une maladie auto-immune
US20190367904A1 (en) * 2016-11-07 2019-12-05 Zymo Research Corporation Automated method for release of nucleic acids from microbial samples

Also Published As

Publication number Publication date
WO2021026025A3 (fr) 2021-03-18
WO2021026025A2 (fr) 2021-02-11
EP3994283A4 (fr) 2022-10-12
EP3994283A2 (fr) 2022-05-11
AU2020326600A1 (en) 2022-02-24

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