CA3114271C - Osteoclast inhibitors for pain - Google Patents

Abstract

Described herein are compounds that may be used in the treatment of knee pain in a mammal in need thereof, and uses thereof. The compound may be a bisphosphonate.
Exemplary compounds include zoledronic acid and neridronic acid. The mammal in need of treatment, which may be a human being, may have pain intensity of 5 cm or greater measured using the 0-10 cm VAS, and an OARSI Grade 0 or Kellgren and Lawrence Grade 0 or Grade 1 of joint space narrowing.

Description

OSTEOCLAST INHIBITORS FOR PAIN
This application is a divisional of Canadian Patent Application No. 2950443, filed on May 27, 2015.
SUMMARY
[0001] Bisphosphonate compounds are potent inhibitors of osteoclast activity, and are used clinically to treat bone-related conditions such as osteoporosis and Paget's disease of bone; and cancer-related conditions including multiple myeloma, and bone metastases from solid tumors. They generally have low oral bioavailability.

[0002] Patchy osteoporosis and bone marrow edema may result from osteoclast hyperactivity. Zoledronic acid is a potent inhibitor of bone resorption and osteoclast activity. Nitrogen containing bisphosphonates, such as zoledronic acid, also inhibit the mevalonate pathway in the osteoclast thereby interrupting normal osteoclast function.

[0003] It has been discovered that oral dosage forms of bisphosphonate compounds, such as zoledronic acid, can be used to treat or alleviate pain or related conditions.

[0004] Some embodiments include a method of enhancing the oral bioavailability of zoledronic acid comprising orally administering a dosage form containing zoledronic acid in the disodium salt form.

[0005] Some embodiments include a dosage form comprising zoledronic acid in the disodium salt form, wherein the bioavailability, in a mammal, of zoledronic acid in the disodium salt form is greater than the bioavailability of zoledronic acid in the diacid form would be in the same dosage form.

[0006] Some embodiments include a dosage form comprising zoledronic acid, wherein the dosage form contains an amount of zoledronic acid in the disodium salt form that provides an area under the plasma concentration curve of zoledronic acid of about 4 ng=h/mL to about 2000 ng=h/mL to a human being to which the dosage form is administered.

[0007] Some embodiments include a dosage form comprising zoledronic acid in the disodium salt form, wherein the disodium salt form is present in a lower molar amount than would be present if the zoledronic acid were in the diacid form;

Date Recue/Date Received 2021-04-07 and wherein the zoledronic acid in the disodium salt form has an improved bioavailability as compared to the zoledronic acid in the diacid form to the extent that the lower molar amount of the disodium salt in the dosage form does not reduce the amount of zoledronic acid delivered to the plasma of a mammal.

[0008] Although an oral dosage form with enhanced bioavailability with respect to the bisphosphonate compound can be used, the treatment can also be effective using an oral dosage form that includes a bisphosphonate compound, such as zoledronic acid, wherein the bioavailability of the bisphosphonate is unenhanced, or is substantially unenhanced.

[0009] Some embodiments include a method of relieving inflammatory pain comprising administering an oral dosage form containing zoledronic acid to a mammal in need thereof, wherein the mammal experiences significant pain relief more than 3 hours after administration of the dosage form.

[0010] Some embodiments include a method of relieving pain associated with an arthritis comprising administering an oral dosage form containing zoledronic acid to a human being in need thereof.

[0011] Some embodiments include a method of treating complex regional pain syndrome comprising administering an oral dosage form containing zoledronic acid to a mammal in need thereof.

[0012] Some embodiments include an oral dosage form comprising zoledronic acid, wherein the oral bioavailability of zoledronic acid is substantially unenhanced. For example, in some embodiments, the oral bioavailability in the dosage form is about 0.01% to about 4%.

[0013] Some embodiments include a pharmaceutical product comprising more than one unit of an oral dosage form described herein. In some embodiments, each unit of the oral dosage form contains about 1 mg to about 50 mg of zoledronic acid.

[0014] Some embodiments include a method of relieving inflammatory pain comprising administering an oral dosage form containing zoledronic acid to a mammal in need thereof.

[0015] In some embodiments, the mammal receives a total monthly dose of zoledronic acid that is about 800 mg/m2 or less.

Date Recue/Date Received 2021-04-07

[0016] In some embodiments, the dosage form contains about 10 mg/m2 to about 20 mg/m2 based upon the body surface area of the mammal.

[0017] Some embodiments include a method of relieving inflammatory pain comprising orally administering zoledronic acid to a mammal in need thereof.

[0018] In some embodiments, about 300 mg/m2 to about 600 mg/m2 of zoledronic acid is administered per month, based upon the body surface area of the mammal.

[0019] In some embodiments, about 50 mg/m2 to about 600 mg/m2 of zoledronic acid is administered per month, based upon the body surface area of the mammal.
[0019a] In one embodiment, there is provided use of a compound for the treatment of knee pain, in a mammal in need thereof, wherein the mammal has pain intensity of 5 cm or greater measured using the 0-10 cm visual analog scale (VAS), and an OARSI Grade 0 or Kellgren and Lawrence Grade 0 or Grade 1 of joint space narrowing; and wherein the compound is a bisphosphonate.
[0019b] In one embodiment, there is provided use of a compound in the manufacture of a medicament for the treatment of knee pain, in a mammal in need thereof, wherein the mammal has pain intensity of 5 cm or greater measured using the 0-10 cm VAS, and an OARSI Grade 0 or Kellgren and Lawrence Grade 0 or Grade 1 of joint space narrowing; and wherein the compound is a bisphosphonate.
[0019c] In one embodiment, there is provided a compound for use in the treatment of knee pain in a mammal in need thereof, wherein the mammal has pain intensity of 5 cm or greater measured using the 0-10 cm VAS, and an OARSI
Grade 0 or Kellgren and Lawrence Grade 0 or Grade 1 of joint space narrowing; and wherein the compound is a bisphosphonate.

Date Recue/Date Received 2021-04-07 BRIEF DESCRIPTION OF DRAWINGS

[0020] FIG. 1 is a plot of pain compression thresholds in a rat model of inflammatory pain using three different doses of zoledronic acid. Measurements were taken at baseline (BL) and at various time points after dosing on the days indicated.

[0021] FIG. 2A is a graph depicting reversal of arthritis pain for two different doses of zoledronic acid in a rat model of arthritis pain.

[0022] FIG. 2B is a graph depicting pain thresholds for two different doses of zoledronic acid in a rat model of arthritis pain.

[0023] FIG. 3 is a graph summarizing the results for vehicle and zoledronic acid treated rats in a rat model of complex regional pain syndrome.

0024] FIG. 4 depicts hindpaw pain thresholds for vehicle and zoledronic acid treated rats in a rat model of complex regional pain syndrome.

[0025] FIG. 5 depicts weight bearing for vehicle and zoledronic acid treated rats in a rat model of complex regional pain syndrome.

[0026] FIG. 6 depicts paw thickness change for vehicle and zoledronic acid treated rats in a rat model of complex regional pain syndrome.

[0027] FIG. 7 depicts the aqueous solubility of disodium zoledronate tetrahydrate as compared to the diacid form of zoledronic acid.
3a Date Recue/Date Received 2021-04-07

[0028] FIG. 8 depicts the plasma concentration of zoledronic acid in dogs over time after administration of 150 mg of the disodium salt form of zoledronic acid and the diacid form of zoledronic acid.

[0029] FIG. 9 depicts the compressibility of dosage forms containing zoledronic acid in the disodium salt form as compared to the diacid form.

[0030] FIG. 10 depicts the change in VAS pain score compared to placebo at three months with zoledronic acid treatment in patients with osteoarthritis of the knee, bone marrow lesions, and different degrees of joint space narrowing.

[0031] FIG. 11 depicts the change in VAS pain score compared to baseline at three months with zoledronic acid treatment in patients with osteoarthritis of the knee, bone marrow lesions, and different degrees of joint space narrowing.

[0032] FIG. 12 depicts the change in VAS pain score compared to placebo at three months with zoledronic acid treatment in different subgroups of patients with osteoarthritis of the knee and bone marrow lesions.

[0033] FIG. 13 depicts the change in BML lesion size compared to placebo at six months with zoledronic acid treatment in patients with osteoarthritis of the knee, bone marrow lesions, and different degrees of joint space narrowing.
DETAILED DESCRIPTION

[0034] Inhibitors of osteoclast activity include bisphosphonate compounds such as pamidronate or pamidronic acid, neridronate or neridronic acid, olpadronate or olpadronic acid, alendronate or alendronic acid, incadronate or incadronic acid, ibandronate or ibandronic acid, risedronate or risedronic acid, cimadronate or cimadronic acid, zoledronate or zoledronic acid, etidronate or etidronic acid, clodronate or clodronic acid, tiludronate or tiludronic acid, etc.

[0035] RANK/RANKL
antagonists may be inhibitors of osteoclast activity.
RANK/RANKL antagonists include but are not limited to OPG (osteoprotegerin) or a variant thereof, an anti-RANKL antibody such as denosumab, a monoclonal anti-RANKL antibody, a small interfering RNA, a microRNA, a precursor molecule, a ribozyme, an antisense nucleic acid, or an aptamer targeting RANKL. Antibodies such as AB-25E9, small molecules, small interfering RNAs, microRNAs, precursor Date Recue/Date Received 2021-04-07 molecules, ribozymes, antisense nucleic acids, or aptamers that target the cell-surface protein Siglec-15 may be osteoclast inhibitors.

[0036] Some Bruton's tyrosine kinase (BTK) inhibitors may be inhibitors of osteoclast activity. BTK
inhibitors can include ONO-4059; ibrutinib; Benzo[b]
thiophene-2-carboxamide, N-[3-[6-[[4-[(2R)-1,4-dimethy1-3-ox0-2-piperazinyl]phenyl]
amino]-4,5-d ihydro-4-methyl-5-oxo-2-pyrazi ny1]-2-methyl pheny1]-4,5,6,7-tetrahydro-(GDC-0834); RN-486; Benzamide, 4-(1,1-dimethylethyl)-N-[3-[8-(phenylamino) imidazo[1,2-a]pyrazin-6-yllphenyll- (CG1-560); Benzamide. N-[344,5-dihydro-4-methy1-6-[[4-(4-morpholinylcarbonyl)phenyl]amino]-5-oxo-2-pyraziny1]-2-methylpheny1]-4-(1,1-dimethylethyl)- (CG1-1746CAS Registry No. 910232-84-7);
HM-71224; 2-Propenamide, N-[34[5-fluoro-24[4-(2-methoxyethoxy)phenyl]amino]-4-pyrimidinyl]amino]phenyll- (CC-292, CAS Registry No. 1202757-89-8); 2-Pyridinecarboxamide, 4444[5-fluoro-44[3-[(1-oxo-2-propen-1-yl)amino]phenyl]amino]
-2-pyrimidinyl]aminolphenoxyl-N-methyl- (CNX-774, CAS Registry No. 1202759-32-7), AVL-101 (CAS Registry No. 1552307-34-2), AVL-291 (CAS Registry No.
1552307-35-3), and AVL-292 (CAS Registry No. 1552307-36-4), [N-(2-chloro-6-methylpheny1)-2-(6-(4-(2-hydroxyethyl) pi perazin-1-y1)-2-methyl pyrimidin-4-ylamino)thiazole-5-carboxamide] (dasatinib), alpha-cyano-beta-hydroxy-beta-methyl-N-(2,5-bromophenyl) propenamide (LFM-A13), and ONO-WG-307.
Date Recue/Date Received 2021-04-07 0 Br Br N
,N

lbrutinib 0 ¨N N¨

I \
HN =
= 0 N' /)NH
/N ____________________________________ \

z.SNr=N
/
NN
CI

Date Recue/Date Received 2021-04-07 Dasatinib HN
\, NJ

N

NNN
N - =IN

[0037] Inhibitors of osteoclast activity may be used for a number of medical purposes, such as treatment of undesirable conditions or diseases, including pain relief. This may be accomplished in many instances by administration of oral dosage forms. Generally, an oral dosage form comprising a bisphosphonate such as Date Recue/Date Received 2021-04-07 zoledronic acid is administered orally to a mammal, such as a human being, at least once, to treat a disease or condition, or to relieve pain.

[0038] The following compounds may also be osteoclast inhibitors:
¨+

HO OH
Compound 1 ¨+

P031-12 ___________________________ HO
Compound 2

[0039] The term "treating" or "treatment" broadly includes any kind of treatment activity, including the diagnosis, cure, mitigation, or prevention of disease in man or other animals, or any activity that otherwise affects the structure or any function of the body of man or other animals.

[0040] An oral dosage form of a bisphosphonate such as zoledronic acid may be used to treat, or provide relief of, any type of pain including, but not limited to, inflammatory pain, arthritis pain, complex regional pain syndrome, lumbosacral pain, musculoskeletal pain, neuropathic pain, chronic pain, cancer-related pain, acute pain, postoperative pain, etc. In some instances, pain relief may be palliative, or pain relief may be provided independent of improvement of the disease or condition or the underlying cause of the disease or condition. For example, although the underlying disease may not improve, or may continue to progress, an individual suffering from the disease may experience pain relief. In some embodiments, enhanced bioavailability of the zoledronic acid may be achieved in treating one of these conditions by administering a dosage form comprising zoledronic acid in the form of a disodium salt. This may allow a reduced molar amount of the disodium salt to be used as compared to what would be used with the diacid form.

Date Recue/Date Received 2021-04-07

[0041] In some embodiments, the mammal being treated is not suffering from bone metastasis. In some embodiments, the mammal being treated is not suffering from cancer. In some embodiments, the mammal being treated is not suffering from osteoporosis.

[0042] For example, zoledronic acid or another bisphosphonate may be administered orally to relieve musculoskeletal pain including low back pain, and pain associated with rheumatoid arthritis, juvenile rheumatoid arthritis, osteoarthritis, erosive osteoarthritis, sero-negative (non-rheumatoid) arthropathies, non-articular rheumatism, peri-articular disorders, axial spondyloarthritis including ankylosing spondylitis, Paget's disease, fibrous dysplasia, SAPHO syndrome, transient osteoarthritis of the hip, vertebral crush fractures, osteoporosis, etc. In some embodiments, enhanced bioavailability of the zoledronic acid may be achieved in treating one of these conditions by administering a dosage form comprising zoledronic acid in the form of a disodium salt. This may allow a reduced molar amount of the disodium salt to be used as compared to what would be used with the diacid form.

[0043] An osteoclast inhibitor, such as a bisphosphonate, e.g. zoledronic acid, may also be used to treat bone fractures or to enhance the healing of bone fractures.

[0044] In some embodiments, zoledronic acid or another bisphosphonate may also be administered orally to relieve neuropathic pain, including diabetic peripheral neuropathy, post-herpetic neuralgia, trigeminal neuralgia, monoradiculopathies, phantom limb pain, and central pain. Other causes of neuropathic pain include cancer-related pain, lumbar nerve root compression, spinal cord injury, post-stroke pain, central multiple sclerosis pain, HIV-associated neuropathy, and radio-therapy or chemo-therapy associated neuropathy. In some embodiments, enhanced bioavailability of the zoledronic acid may be achieved in treating one of these conditions by administering a dosage form comprising zoledronic acid in the form of a disodium salt. This may allow a reduced molar amount of the disodium salt to be used as compared to what would be used with the diacid form.

Date Recue/Date Received 2021-04-07

[0045] In some embodiments, zoledronic acid or another bisphosphonate may be administered orally to relieve inflammatory pain including musculoskeletal pain, arthritis pain, and complex regional pain syndrome. In some embodiments, enhanced bioavailability of the zoledronic acid may be achieved in treating one of these conditions by administering a dosage form comprising zoledronic acid in the form of a disodium salt. This may allow a reduced molar amount of the disodium salt to be used as compared to what would be used with the diacid form.

[0046] Examples of musculoskeletal pain include low back pain; and pain associated with vertebral crush fractures, fibrous dysplasia, osteogenesis imperfecta, Paget's disease of bone, transient osteoporosis, and transient osteoporosis of the hip.

[0047] Arthritis refers to inflammatory joint diseases that can be associated with pain. Examples of arthritis pain include pain associated with osteoarthritis, erosive osteoarthritis, rheumatoid arthritis, juvenile rheumatoid arthritis, sero-negative (non-rheumatoid) arthropathies, non-articular rheumatism, peri-articular disorders, neuropathic arthropathies including Charcot's foot, axial spondyloarthritis including ankylosing spondylitis, and SAPHO syndrome.

[0048] In some embodiments, a human being that is treated for a disease or condition, such as an inflammatory condition, e.g. arthritis, by an osteoclast inhibitor, such as a bisphosphonate, e.g. an oral dosage form of zoledronic acid, has an age of about 10 years to about 90 years, about 20 years to about 80 years, about 30 years to about 75 years old, about 40 years to about 70 years, about 1 year to about 16 years, or about 80 years to about 95 years.

[0049] n some embodiments, a human being that is treated for a disease or condition, such as an inflammatory condition, e.g. arthritis, by an osteoclast inhibitor, such as a bisphosphonate, e.g. an oral dosage form of zoledronic acid, has suffered from the arthritis for at least 1 month, at least 2 months, at least 6 months, or at least 1 year.

[0050] In some embodiments, the arthritis affects a knee, an elbow, a finger, a wrist, a shoulder, an ankle, the spine, or a hip.
lo Date Recue/Date Received 2021-04-07

[0051] For treatment of arthritis or joint pain, such as knee pain, in some embodiments the person being treated has OARS! Grade 0, or Kellgren and Lawrence Grades 0 or 1, joint space narrowing.

[0052] In some embodiments, the person has lesions, such as bone marrow lesions. In some embodiments the person being treated for bone marrow lesions has normal joint space knee pain, OARS! Grade 0, or Kellgren and Lawrence Grades 0 or 1, joint space narrowing.

[0053] In some embodiments, the person has baseline pain intensity of 5 or greater measured using the 0-10 numerical rating scale (NRS), or 50 mm or greater using the 100 mm visual analog scale (VAS). In some embodiments the person being treated for pain has normal joint space knee pain, OARS! Grade 0, or Kellgren and Lawrence Grades 0 or 1, joint space narrowing.

[0054] Bone marrow lesions (BMLs) include regional bone marrow signal intensity alterations on magnetic resonance imaging (MRI). BMLs can be present in the knee and can be an important feature of osteoarthritis of the knee. BMLs have also been described in other rheumatic conditions such as rheumatoid arthritis, osteonecrosis, ankylosing spondylitis, and transient osteoporosis of the hip and are often referred to as bone marrow edema (BME).

[0055] In some embodiments, a person being treated for arthritis, such as with zoledronic acid, has osteoarthritis of the knee associated with bone marrow lesions.

[0056] In some embodiments, an inhibitor of osteoclast activity can be used to treat bone marrow lesions.

[0057] In some embodiments, an inhibitor of osteoclast activity can be used to treat bone marrow lesions of the knee, shoulder, ankle, wrist, hand, fingers, spine, or hip.

[0058] Commonly used measures of pain intensity include the visual analog scale (VAS) and the numerical rating scale (NRS). With the VAS
approach, patients rate the severity of their pain by marking a point on a 10-cm (or 100 mm) VAS (0=no pain and 10=worst possible pain). With the NRS approach, patients rate the severity of their pain by verbally responding to a 10-point NRS (0=no pain and 10=worst possible pain). VAS and NRS scores have been shown to be strongly Date Recue/Date Received 2021-04-07 correlated (slope of regression line, 1.01), indicating that a score on the 10-cm VAS
is equivalent to the same score on 10-point NRS (Bijur PE et al. Acad Emerg Med 2003; 10:390-392). For example, a VAS score of 5 cm (or 50 mm) is equivalent to an NRS score of 5. Knee pain in a person with a VAS score of 5 cm or 50 mm or higher, or an NRS score of 5 or higher, may be referred to herein as moderate to severe knee pain.

[0059] In some embodiments, the patient suffering from pain, inflammation, a similar condition, or any of the conditions described herein, has an NRS of 5 or greater, or a VAS of 5 cm or greater. In some embodiments, the patient has an NRS of 4 or greater, or a VAS of 4 cm or greater. In some embodiments, the patient has an NRS of 6 or greater, or a VAS of 6 cm or greater. In some embodiments, the patient has an NRS of 7 or greater, or a VAS of 7 cm or greater. In some embodiments, the patient has an NRS of about 1, about 2, about 3, about 4, about 5, about 6, about 7, about 8, about 9, or about 10. In some embodiments, the patient has a VAS of about 1 cm, about 2 cm, about 3 cm, about 4 cm, about 5 cm, about 6 cm, about 7 cm, about 8 cm, about 9 cm, or about 10 cm.

[0060] For knee pain or pain associated with bone marrow lesions, in some embodiments, treatment with a nitrogen-containing bisphosphonate such as zoledronic acid may decrease the visual analog (VAS) pain score measured using a 100 mm scale, by at least about 5 mm, at least about 8 mm, at least about 10 mm, at least about 15 mm, up to about 50 mm, or up to about 100 mm. In some embodiments, the VAS score, may be decreased by at least about 5 mm, at least about 8 mm, at least about 10 mm, at least about 15 mm, up to about 50 mm, or up to about 100 mm, as compared to a placebo.

[0061] Treatment with a nitrogen-containing bisphosphonate such as zoledronic acid may decrease the numerical rating scale (NRS) pain score measured using a 0-10 scale, by at least about 0.1, at least about 0.5, at least about 0.8, at least about 1, at least about 1.5, up to about 5, or up to about 10. In some embodiments, the NRS score may be decreased by at least about 0.1, at least about 0.5, at least about 0.8, at least about 1, at least about 1.5, up to about 5, or up to about 10, as compared to a placebo.

Date Recue/Date Received 2021-04-07

[0062] In some embodiments, an inhibitor of osteoclast activity can be used to reduce the size of bone marrow lesions. The area of the lesions may be measured as the total area of all lesions or as the area of any one lesion. In some embodiments, the total area includes the medial tibial area, the medial femoral area, the lateral tibial area, and the lateral femoral area. In some embodiments the bone marrow lesion in located in the patella.

[0063] In some embodiments, the use of an inhibitor of osteoclast activity achieves a reduction in the total area of the bone marrow lesions of at least about 240 mm2. In some embodiments, the reduction in total area is at least about mm2, at least about 200 mm2, at least about 150 mm2, at least about 100 mm2, or at least about 50 mm2. In some embodiments, the reduction in size of bone marrow lesions represents a reduction relative to baseline of at least about 10%, at least about 20%, at least about 30%, at least about 40%, at least about 50%, at least about 60%, at least about 70% at least about 80%, at least about 90%, or about 100%. In some embodiments, the reduction in area of bone marrow lesions represents an improvement relative to placebo of at least about 10%, at least about 20%, at least about 30%, at least about 40%, at least about 50%, at least about 60%, at least about 70%, at least about 80%, at least about 90%, at least about 100%, at least about 120%, at least about 150%, at least about 170%, at least about 200%, at least about 250%, at least about 300%, at least about 350%, at least about 400%, or at least about 450%. In some embodiments, the use of an inhibitor of osteoclast activity inhibits an increase in the size of the bone marrow lesions over time.

[0064] Joint space narrowing (JSN) is typically graded using the Osteoarthritis Research Society International (OARS!) atlas criteria, or the Kellgren and Lawrence (K/L) system. The OARSI atlas criteria grades JSN using a 0-3 scale with Grade 0 indicating an absence of JSN, and Grades 1, 2 and 3 indicating mild, moderate, and severe JSN, respectively (Altman and Gold, Osteoarthritis Cartilage 2007;15(Suppl A):A1-A56). The K/L system grades JSN using a 0-4 scale with Grade 0 indicating an absence of JSN, Grade 1 indicating doubtful JSN, and grades 2, 3 and 4 indicating minimal, moderate, and severe JSN, respectively (Kellgren and Lawrence , Ann Rheum Dis 1957;16:494-502). Based on these criteria, OARS!
Grade 0 (absence of JSN), approximates K/L Grades 0-1 (absence of, or doubtful Date Recue/Date Received 2021-04-07 presence of JSN). Knee pain in a person having OARS! Grade 0 or K/L Grade or 1 JSN in the knee where the pain occurs may be referred to herein as a "normal joint space knee pain."

[0065] In some embodiments for patients having OARS! Grade 0 or K/L
Grades 0-1 JSN, the use of an inhibitor of osteoclast activity achieves a reduction in the total area of the bone marrow lesions of at least about 240 mm2. In some embodiments, the reduction in total area is at least about 220 mm2, at least about 200 mm2, at least about 150 mm2, at least about 100 mm2, or at least about 50 mm2.
In some embodiments, the reduction in size of bone marrow lesions represents a reduction relative to baseline of at least about 10%, at least about 15%, at least about 20%, at least about 25%, at least about 30%, at least about 35%, at least about 40%, at least about 45%, at least about 50%, at least about 60%, at least about 70% at least about 80%, at least about 90%, or about 100%. In some embodiments, the reduction in area of bone marrow lesions represents an improvement relative to placebo of at least about 10%, at least about 20%, at least about 30%, at least about 40%, at least about 50%, at least about 60%, at least about 70%, at least about 80%, at least about 90%, at least about 100%, at least about 120%, at least about 150%, at least about 170%, at least about 200%, at least about 250%, at least about 300%, at least about 350%, at least about 400%, or at least about 450%. In some embodiments, the use of an inhibitor of osteoclast activity inhibits an increase in the size of bone marrow lesions over time.

[0066] In some embodiments for patients having OARS! Grades 1-2 or K/L
Grades 2-4 JSN, the use of an inhibitor of osteoclast activity achieves a reduction in the total area of the bone marrow lesions of at least about 100 mm2. In some embodiments, the reduction in total area is at least about 50 mm2, at least about 60 mm2, at least about 80 mm2, at least about 85 mm2, at least about 90 mm2, at least about 100 mm2, at least about 105 mm2, at least about 110 mm2, or at least about 115 mm2. In some embodiments, the reduction in size of bone marrow lesions represents a reduction relative to baseline of at least about 10%, at least about 20%, at least about 30%, at least about 40%, at least about 50%, at least about 60%, at least about 70% at least about 80%, at least about 90%, or about 100%. In some embodiments, the reduction in area of bone marrow lesions represents an improvement relative to placebo of at least about 10%, at least about 20%, at least Date Recue/Date Received 2021-04-07 about 30%, at least about 40%, at least about 50%, at least about 60%, at least about 70%, at least about 80%, at least about 90%, at least about 100%, at least about 115%, at least about 125%, at least about 135%, at least about 150%, at least about 170%, at least about 200%, at least about 250%, at least about 300%, at least about 350%, at least about 400%, or at least about 450%. In some embodiments, the use of an inhibitor of osteoclast activity inhibits an increase in the size of bone marrow lesions over time.

[0067] In some embodiments, an inhibitor of osteoclast activity, such as a nitrogen-containing bisphosphonate, including e.g. zoledronic acid, minodronic acid, etc., is used to treat fibromyalgia.

[0068] According to some embodiments, administration of an inhibitor of osteoclast activity achieves a reduction in pain that lasts at least about one month, two months, three months, four months, six months, or even at least about 12 months. According some embodiments, administration of an inhibitor of osteoclast activity achieves a reduction in pain that is observed at greater than three hours, at about one day, at about two to about five days, at about one week, at about two weeks, at about three weeks, at about one month, at about five weeks, at about six weeks, at about seven weeks, at about two months, at about nine weeks, at about ten weeks, at about eleven weeks, at about three months, at about four months, at about six months, or at about 12 months after administration of the inhibitor of osteoclast activity.

[0069] According some embodiments, administration of an inhibitor of osteoclast activity achieves a reduction in pain that is observed at greater than three hours, but at or before one week, two weeks, three weeks, four weeks, five weeks, six weeks, seven weeks, eight weeks, nine weeks, 10 weeks, 11 weeks, 12 weeks, four months, five months, or six months.

[0070] According some embodiments, administration of an inhibitor of osteoclast activity achieves a reduction in pain that is observed at greater than three hours with a duration of no more than about three months, no more than about four months, no more than about five months, or no more than about six months.

[0071] According to some embodiments, after the administration of an inhibitor of osteoclast activity, the area of bone marrow lesions relative to the size Date Recue/Date Received 2021-04-07 prior to administration remains reduced for up to three months, four months, five months, six months, or even up to 12 months or more. According to some embodiments, after the administration of an inhibitor of osteoclast activity, the area of bone marrow lesions relative to the size prior to administration is reduced at about three months, at about four months, at about five months, at about six months, or at about 12 months.

[0072] According to some embodiments, after administration of an inhibitor of osteoclast activity, the size of Modic changes or VESCs relative to the size prior to administration remains reduced for up to three months, four months, five months, six months, or even up to 12 months or more. According to some embodiments, after the administration of an inhibitor of osteoclast activity, the size of Modic changes or VESCs relative to the size prior to administration is reduced at about three months, at about four months, at about five months, at about six months, or at about months.

[0073] In some embodiments, an osteoclast inhibitor, such as a nitrogen-containing bisphosphonate, e.g. zoledronic acid, ibandronic acid or minodronic acid, may be administered to relieve complex regional pain syndrome, such as complex regional pain syndrome type I (CRPS-I), complex regional pain syndrome type II

(CRPS-I1), CRPS-NOS, or another type of CRPS.

[0074] In some embodiments, zoledronic acid or another bisphosphonate may be administered orally to relieve complex regional pain syndrome, such as complex regional pain syndrome type I (CRPS-I), complex regional pain syndrome type II (CRPS-I1), CRPS-NOS, or another type of CRPS. CRPS is a type of inflammatory pain. CRPS can also have a neuropathic component.

[0075] Complex regional pain syndrome is a debilitating pain syndrome. It is characterized by severe pain in a limb that can be accompanied by edema, and autonomic, motor and sensory changes.

[0076] In some embodiments, an osteoclast inhibitor, such as a nitrogen-containing bisphosphonate, e.g. zoledronic acid or minodronic acid, may be used to reduce the use of non-steroidal anti-inflammatory drug (NSAIDs), opioids, or other pain medications, for a patient suffering from pain, inflammation, a similar condition, or any condition described herein. For example, use of NSAIDs, opioids, or other Date Recue/Date Received 2021-04-07 pain medications may be reduced by at least about 5%, at least about 10%, at least about 15%, at least about 20%, at least about 25%, at least about 30%, at least about 35%, at least about 40%, at least about 45%, at least about 50%, at least about 60%, at least about 70%, at least about 80%, or at least about 90%, up to about 100%, as compared to the use of NSAIDs, opioids or other pain medications without administration of the osteoclast inhibitor. Use of the opioids, NSAIDs, or other pain medications may be reduced by at least about 5%, at least about 10%, at least about 15%, at least about 20%, at least about 25%, at least about 30%, at least about 35%, at least about 40%, at least about 45%, at least about 50%, at least about 60%, at least about 70%, at least about 80%, or at least about 90%, up to about 100%, as compared to the use of NSAIDS, opioids, or other pain medications at baseline.

[0077] The reduction in the use of NSAIDs, opioids, or other pain medications may be observed at about one week, about two weeks, about three weeks, about one month, about two months, about three months, about four months, about five months, about six months, about seven months, about eight months, about nine months, about 10 months, about 11 months, or about one year or more, after the administration of osteoclast inhibitor.

[0078] With respect to use of oral zoledronic acid for relieving pain associated with an inflammatory condition, relief of pain can be short-term, e.g. for a period of hours after administration of the dosage form, and/or relief of pain can be long-term, e.g. lasting for days, weeks, or even months after oral administration of zoledronic acid. In some embodiments, a mammal, such as a human being, experiences significant pain relief at least about 3 hours, at least about 6 hours, at least about 12 hours, at least about 24 hours, at least about 48 hours, at least about one week, at least about 2 weeks, or at least about 3 weeks after administration of an oral dosage form comprising zoledronic acid. In some embodiments, a mammal, such as a human being, experiences significant pain relief during at least part of the time from about 3 hours to about 2 weeks, about 3 hours to about 3 weeks, about 3 hours to about 24 hours, about 6 hours to about 2 weeks, or about 6 hours to about 24 hours, about 3 days to about 2 weeks, about 6 days to about 2 weeks, after administration of an oral dosage form comprising zoledronic acid. In some embodiments, a human being treated has significant pain relief at three months, six Date Recue/Date Received 2021-04-07 months, nine months, or one year after administration of the most recent dose of an osteoclast inhibitor such as zoledronic acid.

[0079] With respect to the treatment of any condition recited herein, in some embodiments a first oral dosage form comprising zoledronic acid is administered and a second oral dosage form comprising oral zoledronic acid is administered. The timing of the administration of the two dosage forms may be such that, with respect to the first oral dosage form, the second oral dosage with respect to the first oral dosage form, the second oral dosage form is administered at 5 x Tmax or greater (e.g., if Tmax is 1 hour, at 5 hours or later), at least 10 x Tmax or greater, at least about 15 x Tmax or greater, at least about 20 x Tmax or greater, at least about 50 X Trnax or greater, or at least about 200 x Tmax or greater, wherein Tõ,, is the time of maximum plasma concentration for the first oral dosage

[0080] Some embodiments include treatment of a condition recited herein, such as inflammatory pain, arthritis, or complex regional pain syndrome, wherein the treatment comprises either: administering only one dosage form to a mammal to treat the condition, or administering a first dosage form to the mammal, followed by administering a second dosage form to the mammal. If two or more dosage forms are administered, the second oral dosage form is administered before the maximum pain relieving effect of the first oral dosage form is achieved, or before a peak in the pain relieving effect of the first oral dosage form is experienced by a mammal, receiving the dosage form. In some embodiments, the second oral dosage form is administered before an observable pain relieving effect is achieved. In some embodiments, the second dosage form is administered about 12 hours to about 60 days, about 24 hours to about 28 days, about 24 hours to about 7 days, about hours to about 14 days, or about 24 hours to about 21 days, after the first dosage form is administered.

[0081] Some embodiments include treatment of a condition recited herein, such as inflammatory pain, arthritis, or complex regional pain syndrome, wherein the treatment comprises administering a first dosage form to the mammal, followed by administering a second dosage form to the mammal, wherein the second dosage form is administered after the maximum pain relieving effect of the first oral dosage form is achieved, and the second oral dosage form is administered while the mammal is still experiencing pain relief from the first oral dosage form, or while the Date Recue/Date Received 2021-04-07 pain relieving effect from the first oral dosage form is observable. In some embodiments, the second dosage form is administered about 12 hours to about 60 days, about 24 hours to about 28 days, about 24 hours to about 7 days, about hours to about 14 days, or about 24 hours to about 21 days, after the first dosage form is administered.

[0082] Zoledronic acid or another bisphosphonate may also be administered orally to relieve cancer-related pain, including pain associated with multiple myeloma and bone metastases from solid tumors. In some embodiments, zoledronic acid is used to treat pain that is not cancer-related pain. For example, zoledronic acid may be used to treat pain that is not associated with multiple myeloma, bone metastasis from solid tumors, hypercalcemia of malignancy, giant cell tumor of bone, blood cancers or leukemias, or solid tumors or cancers. In some embodiments, enhanced bioavailability of the zoledronic acid may be achieved in treating one of these conditions by administering a dosage form comprising zoledronic acid in the form of a disodium salt. This may allow a reduced molar amount of the disodium salt to be used as compared to what would be used with the diacid form.

[0083] In addition to relieving pain, oral administration of zoledronic acid or another bisphosphonate may also be useful to treat diseases or conditions that may or may not include a pain component. For example, zoledronic acid or another bisphosphonate may be useful to treat any of the pain conditions or types of conditions listed above, including treatment that does not simply relieve the pain of those conditions, and treatment that is carried out in such a way that the condition is treated without pain relief occurring. In addition to any pain relief zoledronic acid or another bisphosphonate may or may not provide, zoledronic acid or another bisphosphonate may be used to treat a disease or condition such as a metabolic disease or condition; an inflammatory disease or condition, including an inflammatory disease or condition that is not associated with pain; a cancer disease or condition; a neurological disease or condition; etc. In some embodiments, enhanced bioavailability of the zoledronic acid may be achieved in treating one of these conditions by administering a dosage form comprising zoledronic acid in the form of a disodium salt. This may allow a reduced molar amount of the disodium salt to be used as compared to what would be used with the diacid form.

Date Recue/Date Received 2021-04-07

[0084] In some embodiments, oral administration of zoledronic acid or another bisphosphonate may also be useful to treat complex regional pain syndrome, rheumatoid arthritis, osteoarthritis, erosive osteoarthritis, axial spondyloarthritis including ankylosing spondylitis, acute vertebral crush fracture, fibrous dysplasia, SAPHO syndrome, osteoporosis, transient osteoporosis, or transient osteoporosis of the hip. In some embodiments, enhanced bioavailability of the zoledronic acid may be achieved in treating one of these conditions by administering a dosage form comprising zoledronic acid in the form of a disodium salt. This may allow a reduced molar amount of the disodium salt to be used as compared to what would be used with the diacid form.

[0085] In some embodiments, oral administration of zoledronic acid or another bisphosphonate may also be useful to treat hypercalcemia of malignancy, multiple myeloma, bone metastases from solid tumors, Paget's disease of bone, giant cell tumor of bone, blood cancers or leukemias, or solid tumors or cancers. In some embodiments, enhanced bioavailability of the zoledronic acid may be achieved in treating one of these conditions by administering a dosage form comprising zoledronic acid in the form of a disodium salt. This may allow a reduced molar amount of the disodium salt to be used as compared to what would be used with the diacid form.

[0086] Some nitrogen-containing bisphosphonates may be represented by Formula A:

0 )c 0 OH HO/ OH
Formula A

[0087] With respect to Formula A, R1 is F, Cl, Br, H, or OH. In some embodiments, R1 is OH.

[0088] With respect to Formula A, R2 is aminoalkyl, such as aminoethyl, aminopropyl, aminopentyl, dimethylaminoethyl, methylpentylaminoethyl, etc; or optionally substituted heterocyclyl alkyl, such as optionally substituted imidazolylmethyl, optionally substituted pyridinymethyl, etc. In some embodiments R2 is optionally substituted imidazolylalkyl.
Date Recue/Date Received 2021-04-07

[0089] Unless otherwise indicated, when a compound or chemical structural feature such as heterocyclyl alkyl is referred to as being "optionally substituted," it includes a feature that has no substituents (i.e.
unsubstituted), or a feature that is substituted, meaning that the feature has one or more substituents.
The term "substituent" has the broadest meaning known to one of ordinary skill in the art, and includes a moiety that replaces one or more hydrogen atoms in a parent compound or structural feature. The term "replaces" is merely used herein for convenience, and does not require that the compound be formed by replacing one atom with another. In some embodiments, a substituent may be any ordinary organic moiety known in the art, which may have a molecular weight (e.g. the sum of the atomic masses of the atoms of the substituent) of 15 g/mol to 50 g/mol, 15 g/mol to 100 g/mol, 15 g/mol to 150 g/mo1,15 g/mol to 200 g/mol, 15 g/mol to 300 g/mol, or 15 g/mol to 500 g/mol. In some embodiments, a substituent comprises, or consists of: 0-30, 0-20, 0-10, or 0-5 carbon atoms; and 0-30, 0-20, 0-10, or 0-5 heteroatoms, wherein each heteroatom may independently be: N, 0, P, S, Si, F, Cl, Br, or I;

provided that the substituent includes one C, N, 0, P, S, Si, F, Cl, Br, or I
atom. In some embodiments, substituents can independently have a molecular weight of about 15 Da to about 600 Da and can consist of 2 to 5 chemical elements, wherein the chemical elements are independently C, H, 0, N, P, S, Si, F, Cl, or Br. In some embodiments, a substituent is optionally substituted alkyl, ¨0-alkyl (e.g.
¨OCH3, -0C2H5, -0C3H7, -0C4H9, etc.), -S-alkyl (e.g. ¨SCH3, -SC2H5, -SC3H7, -SC41-19, etc.), -NR'R", ¨OH, -SH, -CN, -CF3, -NO2, perfluoroalkyl, optionally substituted aryl, optionally substituted heteroaryl, optionally substituted amine or a halogen, wherein R' and R" are independently H or optionally substituted alkyl. Wherever a substituent is described as "optionally substituted," that substituent can be substituted with the above substituents.

[0090] For convenience, the term "molecular weight" is used with respect to a moiety or part of a molecule to indicate the sum of the atomic masses of the atoms in the moiety or part of a molecule, even though it may not be a complete molecule.

[0091] Examples of nitrogen-containing bisphosphonates include but are not limited to pamidronic acid, incadronic acid, ibandronic acid, risedronic acid, Date Recue/Date Received 2021-04-07 minodronic acid, cimadronic acid, neridronic acid, alendronic acid, olpadronic acid, zoledronic acid, etc.
N¨

O
,OH
1=> HO OH
0---NH 12( HO" X,NH2 \/
P OH P OH
HO ,OH 0 P ) -., OH 0 HO-P-=-0 HO. µ / OH
HO HO/

pamidronic acid incadronic acid ibandronic acid II II
PP., 1-ICY- I 1 __ OH /.= % /OH

P--_, OH ll HN 10 N./../-130H /OH

1 HO. OH HO P-0 HO/ \OH

risedronic acid cimadronic acid minodronic acid

[0092] Zoledronic acid has the structure shown below, and is also referred to as zoledronate.
OH
(:) /
Pi OH
0 7 FiX
OH
D.,,='"
N----- .. __ ' OH
Zoledronic acid Date Recue/Date Received 2021-04-07

[0093] Unless otherwise indicated, any reference to a compound herein, such as zoledronic acid, by structure, name, or any other means, includes pharmaceutically acceptable salts, such as the disodium salt; alternate solid forms, such as polymorphs, solvates, hydrates, etc.; tautomers; or any other chemical species that may rapidly convert to a compound described herein under conditions in which the compounds are used as described herein.

[0094] In some embodiments, zoledronic acid is administered in a dosage form comprising a salt form, such as a salt of a dianion of zoledronic acid.
In some embodiments, zoledronic acid is administered in a dosage form comprising a disodium salt form of zoledronic acid. In some embodiments, zoledronic acid is administered in a sodium salt form, such as a monosodium salt, a disodium salt, a trisodium salt, etc. In some circumstances, use of the disodium salt may be desirable. For example, the disodium salt is much more soluble in water than the diacid form. As a result, in some processes, the disodium salt can be easier to work with than the diacid form. Additionally, the sodium salt may be more bioavailable and/or more rapidly absorbed when taken orally as compared to the diacid form.

[0095] Examples of salts of Compound 1 are shown below:
x-PO3H2 NõN PO3H2 OH OH
M+ ¨ 2M+
0 /=\ PO3H- 0 P03H--oN1/7.\N-õA--P03H-¨ 4M+

OH

Date Recue/Date Received 2021-04-07 wherein X- is any suitable anion, e.g. F, Br, cr, r, OH-, acetate, etc.; and V-is any suitable cation, e.g. Na, K+, NH4, etc. Many other salt forms are also possible.

[0096] In some embodiments, Compound 1 may be further represented by a formula, 0 PO3 Na =

[0097] In some embodiments, Compound 1 may be in a hydrate form.

[0098] In some embodiments, Compound 1 is administered in a dosage form comprising a salt form, such as a zwitterionic form, or a salt of a cation, a monoanion, a dianion, a trianion, etc., of Compound 1.

[0099] Compound 1 can be present in any amount, such as less than about 100% w/w, less than about 50% w/w, less than about 20% w/w, less than about 10% w/w, less than about 1% w/w, less than 0.1% w/w, less than about 0.07%
w/w, less than about 0.05% w/w, less than about 0.04% w/w, less than about 0.03%
w/w, less than about 0.02% w/w; and/or greater than 0% w/w, at least about 0.00000001% w/w, at least about 0.000001% w/w, or at least about 0.00001% w/w, based upon the total amount of zoledronic acid, Compound 1, and Compound 2 present in the composition.

[0100] Examples of salts of Compound 2 are shown below:

Date Recue/Date Received 2021-04-07 ¨ X-PO3H2 /_\
703H2 /_\
+/N--___/)\---P03H2 HO , r-L.P3n2 OH OH

M+
¨ 2M+

PO3H- /_\ P0311- /_\

¨ 4M+ ¨ 4Na+

PO3H- /_\ PO3H- /_\
HO.

OH HO

wherein X is any suitable anion, e.g. F, Br, cr, r, OH-, acetate, etc.; and M+
is any suitable cation, e.g. Na+, K+, NH4+, etc. Many other salt forms are also possible.

[0101] In some embodiments, a salt of compound 2 may be further represented by a formula, PO3HNa _________________ PO3HNa HO NN PO3H Na OH
PO3HNa e0H

[0102] In some embodiments, compound 2 may be in a hydrate form.

[0103] In some embodiments, Compound 2 is administered in a dosage form comprising a salt form, such as a zwitterionic form, or a salt of a cation, a monoanion, a dianion, a trianion, etc., of Compound 2.

[0104] Compound 2 can be present in any amount, such as less than about 100% w/w, less than about 50% w/w, less than about 20% w/w, less than about 10% w/w, less than about 1% w/w, less than about 0.3%, less than about 0.2%, less than 0.1% w/w, less than about 0.08% w/w, less than about 0.07%
WAN, Date Recue/Date Received 2021-04-07 less than about 0.05% w/w, less than about 0.04% w/w, less than about 0.03%
w/w, less than about 0.02% w/w; and/or greater than 0% w/w, at least about 0.00000001% w/w, at least about 0.000001% w/w, or at least about 0.00001% w/w, based upon the total amount of zoledronic acid, Compound 1, and Compound 2 present in the composition.

[0105] In some embodiments, Compound 1 and Compound 2 are present in an amount that is less than 0.1% w/w.

[0106] In some embodiments, the administration of an osteoclast inhibitor, such as a nitrogen-containing bisphosphonate, including, e.g. zoledronic acid, minodronic acid, etc., to a patient or mammal in need thereof affects Modic changes (MCs). For example, any of the above compounds could be used to treat Modic changes, or vertebral endplate signal changes (VESC) and bone marrow changes visible using magnetic resonance imaging (MRI), or neck pain or back pain associated with Modic changes.

[0107] Modic changes, as used herein, includes its ordinary meaning in the art and refers to pathological vertebral endplate and bone marrow changes visible using magnetic resonance imaging (MRI). Modic changes may also be referred to as vertebral endplate signal changes (VESC). Modic changes, can be classified into various types including type 1 (M1), type 2 (M2), and type 3 (M3) lesions or changes, any of which may be treated using an osteoclast inhibitor, such as a nitrogen-bisphosphonate, including, e.g. zoledronic acid, minodronic acid, etc.
Different types of Modic changes may occur in the same patient, for example type 1 and type 2 Modic changes (M1/2). In some cases, M1 changes are related to lower back pain than other types of Modic change.

[0108] VESCs may be found in patients with different types of low back pain including but not limited to spondylitis, trauma, spondyloarthropathies including ankylosing spondylitis, Schmorl's nodes, fracture, tumor, and spinal cord infarction.
Lesions in ankylosing spondylitis include osteitis and spondylodiscitis, which can be detected using MRI or another medical imaging instrument.

[0109] Modic changes may be found in the cervical, thoracic, lumbar, and sacral spine. Modic changes may be found at various spinal levels such as at C1/2, C2/3, C3/4, C4/5, C5/6, C6/7, C7/11, 11/2, 12/3, T3/4, 14/5, 15/6, T6/7, 17/8, 18/9, Date Recue/Date Received 2021-04-07 T9/10, 110/11, T11/12, 112/L1, L1/2, L2/3, L3/4, L4/5, L5/S1, etc., any of which may be treated using an osteoclast inhibitor, such as a nitrogen-bisphosphonate, including, e.g. zoledronic acid, minodronic acid, etc.

[0110] In some embodiments, the Modic change being treated is located at L2/3. In some embodiments, the Modic change being treated is located at L3/4.
In some embodiments, the Modic change being treated is located at L4/5. In some embodiments, the Modic change being treated is located at L5/S1.

[0111] In some embodiments, the Modic change being treated is located at C3/4. In some embodiments, the Modic change being treated is located in at C4/5. In some embodiments, the Modic change being treated is located in at C5/6. In some embodiments, the Modic change being treated is located in at C6/7.

[0112] In some embodiments, the Modic change being treated is located at 15/6. In some embodiments, the Modic change being treated is located in at 16/7. In some embodiments, the Modic change being treated is located in at 17/8. In some embodiments, the Modic change being treated is located in at T8/9. In some embodiments, the Modic change being treated is located at 19/10.

[0113] In some embodiments, the patient being treated has predominantly M1. In some embodiments, the patient being treated has predominantly M1/M2. In some embodiments, the patient being treated has predominantly M2. In some embodiments, the patient being treated has predominantly M3.

[0114] In some embodiments, the worst type of lesion that the patient being treated has is M1. In some embodiments, the worst type of lesion that the patient being treated has is M1/2. In some embodiments, the worst type of lesion that the patient being treated has is M2.

[0115] In some embodiments, the patient being treated has Modic changes at more two or more levels. In some embodiments the patient being treated has Modic changes at three or more levels. In some embodiments greater pain relief is obtained when treating a patient with Modic changes at two levels, or three or more levels, than is obtained when treating a patient with Modic changes at a single level or at two levels.

Date Recue/Date Received 2021-04-07

[0116] In some embodiments greater pain relief is obtained when treating a patient with Modic changes at two levels than is obtained when treating a patient with Modic changes at a single level.

[0117] In some embodiments greater pain relief is obtained when treating a patient with Modic changes at three or more levels than is obtained when treating a patient with Modic changes at a single level.

[0118] In some embodiments greater pain relief is obtained when treating a patient with Modic changes three or more levels than is obtained when treating a patient with Modic changes at two levels.

[0119] In some embodiments, the inhibitor of osteoclast activity may be used to effect a reduction in the levels of pro-inflammatory cytokines in the patient with low back pain or any other type of pain or condition recited herein. In some embodiments greater pain relief may be obtained in patients with greater baseline levels of pro-inflammatory cytokines when treated with an inhibitor of osteoclast activity, such as a nitrogen-containing bisphosphonate, including e.g.
zoledronic acid, minodronic acid, etc. In some embodiments, greater pain relief may be obtained in patients who experience a reduction or a greater reduction in the levels of pro-inflammatory cytokines when treated with an inhibitor of osteoclast activity, such as a nitrogen-containing bisphosphonate, including e.g. zoledronic acid, minodronic acid, etc. Pro-inflammatory cytokines include but are not limited to IL-1, IL-2, IL-3, IL-6, IL-8, IL-10, IL-12, tumor necrosis alpha (TNF-alpha), interferon gamma, etc.

[0120] In some embodiments, the use of an inhibitor of osteoclast activity, such as a nitrogen-containing bisphosphonate, including e.g. zoledronic acid, minodronic acid, etc., to a patient or mammal in need thereof, achieves a reduction relative to baseline in the size of Modic changes or VESCs of at least about 5%, at least about 10%, at least about 15%, at least about 20%, at least about 25%, at least about 30%, at least about 40%, at least about 50%, at least about 60%, at least about 70% at least about 80%, at least about 90%, or about 100%. In some embodiments, the reduction the size of Modic changes or VESCs represents an improvement relative to placebo of at least about 10%, at least about 15%, at least about 20%, at least about 25%, at least about 30%, at least about 40%, at least Date Recue/Date Received 2021-04-07 about 50%, at least about 60%, at least about 70%, at least about 80%, at least about 90%, at least about 100%, at least about 120%, at least about 150%, at least about 170%, at least about 200%, at least about 250%, at least about 300%, at least about 350%, at least about 400%, or at least about 450%. In some embodiments, the use of an inhibitor of osteoclast activity inhibits an increase in the size of Modic changes or VESCs over time.

[0121] The oral bioavailability of zoledronic acid may be enhanced by orally administering the zoledronic acid in the disodium salt form. For example, the bioavailability of zoledronic acid may be improved by at least about 10%, at least about 20%, at least about 30%, at least about 50%, and/or up to about 100%, or up to about 200%, as compared to administration of zoledronic acid in the diacid form.

[0122] Because of the improved bioavailability of the disodium salt a dosage form may contain, or a mammal, such as a human being, may receive, on a molar basis, less of the disodium salt form of zoledronic acid than would otherwise be administered of the diacid form of zoledronic acid. For example, a dosage form may contain, or a mammal may receive, at least about 10 mole% less, at least about 20 mole% less, at least about 40 mole% less, at least about 50 mole% less, and/or up to about 90 mole% less or 95 mole% less, of the disodium salt form as compared the amount of the diacid form of zoledronic acid that would otherwise be administered, such as a molar amount that would be administered of zoledronic acid in the diacid form in order to achieve the same plasma levels of zoledronic acid.

[0123] In some embodiments, a dosage form contains, or a mammal (such as a human being) is administered, an amount of the disodium salt form, on a molar basis, that has a value of about 0.8nd to about 1.2nd or about 0.9nd to about 1.1nd, wherein:
nd = (ba/bd)(na) wherein ba is the bioavailability of the diacid form, bd is the bioavailability of the disodium salt form, and na is the number of moles of the diacid that would be administered in a dosage form containing the diacid form of zoledronic acid.
For example, if the diacid form has a bioavailability (ba) of 0.01 and the disodium salt form has a bioavailabity (bd) of 0.015, and a dosage form would normally contain 0.001 moles of the diacid, nd would be (0.01/0.015)(0.001 moles), or about 0.00067 Date Recue/Date Received 2021-04-07 moles. In some embodiments, the disodium salt is administered in an amount that has a value of about nd.

[0124] With respect to oral dosage forms comprising a reduced molar amount of the disodium salt of zoledronic acid as compared to the diacid form of zoledronic acid, in some embodiments, the bioavailability of the zoledronic acid in the disodium salt form is sufficiently high that, if the drug is administered to a mammal, at least as much zoledronic acid is present in the blood of the mammal as would be present if zoledronic acid were administered in the diacid form.

[0125] With respect to oral dosage forms comprising the disodium salt form of zoledronic acid, in some embodiments, the disodium salt form is present in a lower molar amount than would be present if the zoledronic acid were in the diacid form; and the zoledronic acid in the disodium salt form has an improved bioavailability as compared to the zoledronic acid in the diacid form to the extent that the lower molar amount of the disodium salt in the dosage form does not reduce the amount of zoledronic acid delivered to the plasma of a mammal.

[0126] Some oral dosage forms comprising zoledronic acid have a dose of zoledronic acid and a configuration suitable for a particular species of mammal, e.g.
dog, rat, human, etc. Such a dosage form may have zoledronic acid present in an amount that results in a desired range for an area under the plasma concentration curve (AUC) of zoledronic acid in that particular species of mammal. For example the dose of zoledronic acid and a configuration of the oral dosage form may result in an AUC of zoledronic acid of about 1 ng=hr/mL to about 700 ng=hr/mL, about 3 ng=hr/mL to about 30 ng=hr/mL, about 3 ng=hr/mL to about 10 ng=hr/mL, about 50 ng=hr/mL to about 700 ng=hr/mL, about 130 ng=hr/mL to about 180 ng=hr/mL, about 300 ng-hr/mL to about 450 ng-hr/mL, about 300 ng-hr/mL to about 350 ng-hr/mL, about 300 ng-hr/mL to about 310 ng-hr/mL, about 340 ng-hr/mL to about 350 ng=hr/mL, about 370 ng=hr/mL to about 420 ng=hr/mL, about 380 ng-hr/mL to about 390 ng=hr/mL, about 405 ng=hr/mL to about 415 ng=hr/mL, about 140 ng=hr/mL to about 160 ng=hr/mL, about 140 ng=hr/mL to about 150 ng=hr/mL, about 150 ng=hr/mL
to about 160 ng=hr/mL, about 140 ng=hr/mL, 142 ng=hr/mL, about 155 ng=hr/mL, about 305 ng=hr/mL, 304 ng=hr/mL, about 345 ng=hr/mL, 343 ng=hr/mL, about 385 ng=hr/mL, 384 ng=hr/mL, about 410 ng=hr/mL, or any AUC in a range bounded by, or Date Recue/Date Received 2021-04-07 between, any of these values, upon administration of the oral dosage form to a mammal.

[0127] Unless otherwise indicated, the AUC refers to the AUC calculated to the last measured concentration (AUC(o_o)and extrapolated to infinity (AUC(0-int-).

[0128] An oral dosage form comprising zoledronic acid having a dose of zoledronic acid and a configuration suitable for a particular species of mammal may have zoledronic acid present in an amount that results in a Crrax of zoledronic acid of about 0.2 ng/mL to about 300 ng/mL, about 0.5 ng/mL to about 5 ng/mL, about 5 ng/mL to about 300 ng/mL, about 5 ng/mL to about 50 ng/mL, about 20 ng/mL to about 50 ng/mL, about 30 ng/mL to about 50 ng/mL, about 50 ng/mL to about 200 ng/mL, about 50 ng/mL to about 150 ng/mL, about 80 ng/mL to about 120 ng/mL, about 90 ng/mL to about 100 ng/mL, about 50 ng/mL to about 200 ng/mL, about 40 ng/mL, about 95 ng/mL, about 97 ng/mL, or any Cram( in a range bounded by, or between, any of these values, upon administration of the oral dosage form to a mammal.

[0129] An oral dosage form comprising zoledronic acid having a dose of zoledronic acid and a configuration suitable for a particular species of mammal may be configured so that administration of the oral dosage form to the particular species of mammal results in a Tax of zoledronic acid of about 0.4 hr to about 1 hr, about 0.5 hr, or about 0.75 hr, or any Tmax in a range bounded by, or between, any of these values.

[0130] In some embodiments, the zoledronic acid in the disodium salt form is present in an amount such that the oral dosage form provides an area under the plasma concentration curve of zoledronic acid of about 4 ng=h/mL to about 2000 ng=h/mL to the mammal each time the zoledronic acid in the disodium salt is administered.

[0131] In some embodiments, the zoledronic acid is present in an amount such that the oral dosage form provides an area under the plasma concentration curve of zoledronic acid of about 100 ng=h/mL to about 2000 ng=h/mL, about 100 ng-h/mL to about 1000 ng=h/mL, about 500 ng=h/mL to about 1000 ng=h/mL, or about 500 ng=h/mL to about 700 ng=h/mL in the mammal to which the dosage form is Date Recue/Date Received 2021-04-07 administered. This amount may be suitable for administration of the oral dosage form about every 3 to 4 weeks.

[0132] In some embodiments, the zoledronic acid is present in an amount such that the oral dosage form provides an area under the plasma concentration curve of zoledronic acid of about 20 ng=h/mL to about 700 ng=h/mL, about 50 ng=h/mL to about 500 ng=h/mL, about 50 ng=h/mL to about 400 ng=h/mL, about 50 ng=h/mL to about 300 ng=h/mL, about 50 ng=h/mL to about 200 ng=h/mL, about 100 ng=h/mL to about 500 ng=h/mL, about 100 ng=h/mL to about 400 ng=h/mL, about ng=h/mL to about 300 ng=h/mL, about 100 ng=h/mL to about 200 ng=h/mL, about ng-h/mL to about 500 ng=h/mL, about 125 ng=h/mL to about 400 ng=h/mL, about ng-h/mL to about 300 ng=h/mL, about 125 ng=h/mL to about 200 ng=h/mL, or about 200 ng=h/mL to about 300 ng=h/mL, in the mammal to which the dosage form is administered. This amount may be suitable for weekly administration of the oral dosage, or for administration of 3 to 5 individual dosages during a month. The individual dosages could be given at regular intervals, given during the first week, or at any other schedule that provides 3 to 5 dosages during the month.

[0133] In some embodiments, the zoledronic acid is present in an amount such that the oral dosage form provides an area under the plasma concentration curve of zoledronic acid of about 4 ng=h/mL to about 100 ng=h/mL, about 10 ng=h/mL
to about 50 ng=h/mL, about 10 ng=h/mL to about 30 ng=h/mL, 20 ng=h/mL to about 700 ng=h/mL, about 50 ng=h/mL to about 500 ng=h/mL, about 50 ng=h/mL to about 400 ng=h/mL, about 50 ng=h/mL to about 300 ng=h/mL, about 50 ng=h/mL to about 200 ng=h/mL, about 100 ng=h/mL to about 500 ng=h/mL, about 100 ng=h/mL to about 400 ng=h/mL, about 100 ng=h/mL to about 300 ng=h/mL, about 100 ng=h/mL to about 200 ng=h/mL, about 125 ng=h/mL to about 500 ng=h/mL, about 125 ng=h/mL to about 400 ng=h/mL, about 125 ng=h/mL to about 300 ng=h/mL, about 125 ng=h/mL to about 200 ng=h/mL, or about 200 ng=h/mL to about 300 ng=h/mL in the mammal to which the dosage form is administered. This amount may be suitable for daily administration of the oral dosage form. In some embodiments, the dosage form may be administered for 2, 3, 4, 5, 6, 7, 8, 9, or 10, 5 to 10, or 6 to 10 consecutive days.

[0134] Oral administration of zoledronic acid, particularly oral administration of the disodium salt form of zoledronic acid, can result in more Date Recue/Date Received 2021-04-07 sustained plasma levels of the drug as compared to parenteral modes of administration, such intravenous or subcutaneous. For example, the amount of zoledronic acid in the plasma can be significantly higher for oral administration of the disodium salt about 24 hours or 48 hours, or longer, after administration. In some embodiments, oral zoledronic acid has a 24 hour sustained plasma level factor of about 1 or higher, such as about 1 to about 10, about 1 to about 5, about 3 to about 5, or about 3 to about 4. In some embodiments, an orally administered dosage form of zoledronic acid has a 24 hour sustained plasma level factor or a 48 hour sustained plasma level factor that is higher, such as at least 1.2 times, at least about 2 times, at least about 5 times, about 1.2 times to about 20 times, about 2 times to about 15 times, about 5 times to about 10 times, or about 8 to about 15 times that of intravenously administered zoledronic acid. A "sustained plasma level factor,"
pf, is determined by the equation:
pf = 1000 (Ct/Cmax) wherein Cmax is the maximum plasma concentration of zoledronic acid after it is administered and Ct is the plasma concentration of zoledronic acid at the time of interest, such as 24 hours. For parenteral administration, the Cmax can be about the Co, or the concentration right after injection of the entire amount of the drug into the body. Sustained plasma level factors can also be obtained for other times, such as 48 hours, by using the plasma concentration of zoledronic acid for Ct in the equation above. For example, if the maximum plasma level of zoledronic acid after administration is 1000 ng/mL and the plasma level of zoledronic acid at 24 hours is 1 ng/mL, the 24 hour sustained plasma level factor is 1.

[0135] An oral dosage form comprising zoledronic acid having a dose of zoledronic acid and a configuration suitable for a particular species of mammal may be configured so that the zoledronic acid has a 12 hour sustained plasma level factor of about 12 to about 50, about 20 to about 40, about 25 to about 30, about 30 to about 35, about 35 to about 40, about 33, about 30, about 35, or any 12 hour sustained plasma level factor in a range bounded by, or between, any of these values, for the particular species of mammal.

[0136] An oral dosage form comprising zoledronic acid having a dose of zoledronic acid and a configuration suitable for a particular species of mammal may Date Recue/Date Received 2021-04-07 be configured so that the zoledronic acid has a 24 hour sustained plasma level factor of about 10 to about 30, about 10 to about 20, about 10 to about 15, about 12 to about 15 or 16, about 15 to about 20, about 14, about 12, about 15, or any 24 hour sustained plasma level factor in a range bounded by, or between, any of these values, for the particular species of mammal.

[0137] An oral dosage form comprising zoledronic acid having a dose of zoledronic acid and a configuration suitable for a particular species of mammal may be configured so that the zoledronic acid has a 36 hour sustained plasma level factor of about 6 to about 20, about 8 to about 15, about 9 to about 12 or 13, about 8 to about 10, about 11 to about 13, about 9, about 13, or any 24 hour sustained plasma level factor in a range bounded by, or between, any of these values, for the particular species of mammal.

[0138] An oral dosage form comprising zoledronic acid having a dose of zoledronic acid and a configuration suitable for a particular species of mammal may be configured so that the zoledronic acid has a 48 hour sustained plasma level factor of about 5 to about 20, about 6 to about 15, about 7 or 8 to about 12 or 13, about 8 to about 10, about 11 to about 13, about 8, about 12, or any 48 hour sustained plasma level factor in a range bounded by, or between, any of these values, for the particular species of mammal.

[0139] An oral dosage form comprising zoledronic acid having a dose of zoledronic acid and a configuration suitable for a particular species of mammal may be configured so that the zoledronic acid has a 72 hour sustained plasma level factor of about 4 to about 20, about 5 to about 10, about 5 or 6 to about 10 or 11, about 5 to about 6, about 9 to about 10, about 6, about 10, or any 72 hour sustained plasma level factor in a range bounded by, or between, any of these values, for the particular species of mammal.

[0140] An oral dosage form comprising zoledronic acid having a dose of zoledronic acid and a configuration suitable for a particular species of mammal may be configured so that the particular species of mammal has a plasma concentration of zoledronic acid at 12 hours that is about 0.5 ng/mL to about 5 ng/mL, about ng/mL to about 3 ng/mL, about 1 ng/mL to about 2 ng/mL, about 2 ng/mL to about ng/mL, about 3 ng/mL to about 4 ng/mL, about 1.2 ng/mL, about 2.6 ng/mL, about Date Recue/Date Received 2021-04-07 3.2 ng/mL, or any plasma concentration in a range bounded by, or between, any of these values.

[0141] An oral dosage form comprising zoledronic acid having a dose of zoledronic acid and a configuration suitable for a particular species of mammal may be configured so that the particular species of mammal has a plasma concentration of zoledronic acid at 24 hours that is about 0.2 ng/mL to about 2 ng/mL, about 0.5 ng/mL to about 1.5 ng/mL, about 0.5 ng/mL to about 1 ng/mL, about 1 ng/mL to about 1.5 ng/mL, about 0.5 ng/mL, about 1.0 ng/mL, about 1.4 ng/mL, or any plasma concentration in a range bounded by, or between, any of these values.

[0142] An oral dosage form comprising zoledronic acid having a dose of zoledronic acid and a configuration suitable for a particular species of mammal may be configured so that the particular species of mammal has a plasma concentration of zoledronic acid at 36 hours that is about 0.1 ng/mL to about 2 ng/mL, about 0.2 ng/mL to about 1.5 ng/mL, about 0.2 ng/mL to about 0.5 ng/mL, about 0.5 ng/mL
to about 1 ng/mL, about 1 ng/mL to about 1.3 ng/mL, about 0.3 ng/mL, about 0.8 ng/mL, about 1.1 ng/mL, or any plasma concentration in a range bounded by, or between, any of these values.

[0143] An oral dosage form comprising zoledronic acid having a dose of zoledronic acid and a configuration suitable for a particular species of mammal may be configured so that the particular species of mammal has a plasma concentration of zoledronic acid at 48 hours that is about 0.1 ng/mL to about 2 ng/mL, about 0.2 ng/mL to about 1.5 ng/mL, about 0.2 ng/mL to about 0.5 ng/mL, about 0.5 ng/mL
to about 0.9 ng/mL, about 0.9 ng/mL to about 1.3 ng/mL, about 0.3 ng/mL, about 0.7 ng/mL, about 1.1 ng/mL, or any plasma concentration in a range bounded by, or between, any of these values.

[0144] An oral dosage form comprising zoledronic acid having a dose of zoledronic acid and a configuration suitable for a particular species of mammal may be configured so that the particular species of mammal has a plasma concentration of zoledronic acid at 72 hours that is about 0.2 ng/mL to about 1 ng/mL, about 0.2 ng/mL to about 1.5 ng/mL, about 0.1 ng/mL to about 0.3 ng/mL, about 0.3 ng/mL
to about 0.6 ng/mL, about 0.6 ng/mL to about 1 ng/mL, about 0.2 ng/mL, about 0.5 Date Recue/Date Received 2021-04-07 ng/mL, about 0.9 ng/mL, or any plasma concentration in a range bounded by, or between, any of these values.

[0145] An oral dosage form comprising zoledronic acid having a dose of zoledronic acid and a configuration suitable for a particular species of mammal may be configured so that the elimination half-life of zoledronic acid in the particular species of mammal is about 30 hours to about 100 hours, about 40 hours to about 60 hours, about 40 hours to about 50 hours, about 50 hours to about 60 hours, about 42 hours, about 51 hours, about 59 hours, or any half-life in a range bounded by, or between, any of these values.

[0146] As used herein, the "elimination half-life" refers to the apparent first-order terminal plasma elimination half-life, obtained by non-compartmental analysis using Win-Nonlin. A terminal plasma elimination half-life is the time required to reduce the plasma concentration to half after reaching pseudo-equilibrium, and not the time required to eliminate half the administered dose. For orally administered drugs, terminal plasma elimination half-life can be affected by absorption of the drug, as well as plasma clearance and extent of distribution.

[0147] In some embodiments, the disodium salt form of zoledronic acid provides an enhancement to bioavailability, as compared to the diacid form of zoledronic acid, which adds to any enhancement to bioavailability provided by any bioavailability-enhancing agents in the dosage form. In some embodiments, the disodium salt form of zoledronic acid provides an enhancement to bioavailability, as compared to the diacid form of zoledronic acid, which is greater than any enhancement to bioavailability provided by any bioavailability-enhancing agents in the dosage form. In some embodiments, the disodium salt form of zoledronic acid may be administered in a dosage form that is substantially free of bioavailability-enhancing agents.

[0148] In some embodiments, a dosage form comprising a disodium salt of zoledronic acid is a solid.

[0149] In some embodiments, a dosage form comprising a disodium salt of zoledronic acid is used to treat an inflammatory condition.

[0150] In some embodiments, a dosage form comprising a disodium salt of zoledronic acid is used to treat arthritis.

Date Recue/Date Received 2021-04-07

[0151] In some embodiments, a dosage form comprising a disodium salt of zoledronic acid is used to treat complex regional pain syndrome.

[0152] In some embodiments, zoledronic acid is in a form that has an aqueous solubility, meaning the solubility in water, greater than 1% (w/v), about 5%
(w/v) to about 50% (w/v), about 5% (w/v) to about 20% (w/v), about 10% (w/v) to about 15% (w/v), or about 12% (w/v) to about 13% (vviv).

[0153] The disodium salt form of zoledronic acid can be more compressible than the diacid form of zoledronic acid. This can make it easier for a dosage form to have a desired hardness. It can also make it easier to increase the drug load, so that a smaller tablet can be given for a given dosage strength.
In some embodiments, a solid dosage form of zoledronic acid, such as the diacid form of zoledronic acid or the disodium salt form of zoledronic acid, can have a hardness of about 5 kPa to about 20 kPa or about 5 kPa to about 14 kPa.

[0154] Zoledronic acid or another bisphosphonate may be combined with a pharmaceutical carrier selected on the basis of the chosen route of administration and standard pharmaceutical practice as described, for example, in Remington's Pharmaceutical Sciences, 2005. The relative proportions of active ingredient and carrier may be determined, for example, by the solubility and chemical nature of the compounds, chosen route of administration and standard pharmaceutical practice.

[0155] Zoledronic acid or another bisphosphonate may be administered by any means that may result in the contact of the active agent(s) with the desired site or site(s) of action in the body of a patient. The compounds may be administered by any conventional means available for use in conjunction with pharmaceuticals, either as individual therapeutic agents or in a combination of therapeutic agents.
For example, they may be administered as the sole active agents in a pharmaceutical composition, or they can be used in combination with other therapeutically active ingredients.

[0156] In some embodiments, an osteoclast inhibitor is co-administered with a steroid. Suitable steroids include, for example, hydrocortisone, hydrocortisone acetate, cortisone acetate, tixocortol pivalate, prednisolone, methylprednisolone, prednisone, triamcinolone acetonide, triamcinolone alcohol, mometasone, Date Recue/Date Received 2021-04-07 amcinonide, budesonide, desonide, fluocinonide, fluocinolone acetonide, halcinonide, betamethasone, betamethasone sodium phosphate, dexamethasone, dexamethasone sodium phosphate, fluocortolone, hydrocortisone-17-valerate, acleometasone di propionate, betamethasone valerate, betamethasone dippropionate, prednicarbate, clobetasone-17-butyrate, clobetasol-17-propionate, fluocortilone caproate, fluocortolone pivalate, and fluprednidene acetate, hydrocortisone-17-butyrate, 17-aceponate, 17-buteprate, and prednicarbate.

[0157] Any effective dose of steroid can be administered to a person. In some embodiment, the dose of steroids may be about 1 to about 500 mg of the steroid. In some embodiments, the dose of steroids does not exceed the 25 mg of the steroid, and is not less than 5 mg of the steroid.

[0158] The steroid can be given orally (for example, 7.5 mg of prednisone), by a separate infusion (for example, 7.5 mg of methyl prednisolone), mixed in with zoledronic acid in the same infusion, or be administered intramuscularly, subcutaneously, by rectal suppository, by inhalation, or injected directly into a joint.

[0159] Zoledronic acid or another bisphosphonate may be administered to a human patient in a variety of forms adapted to the chosen route of administration, e.g., orally, rectally, or parenterally. Parenteral administration in this respect includes, but is not limited to, administration by the following routes:
pulmonary, intrathecal, intravenous, intramuscular, subcutaneous, intraocular, intrasynovial, transepithelial including transdermal, sublingual and buccal; topically; nasal inhalation via insufflation; and rectal systemic.

[0160] The effective amount of zoledronic acid or another bisphosphonate will vary depending on various factors known to the treating physicians, such as the severity of the condition to be treated, route of administration, formulation and dosage forms, physical characteristics of the bisphosphonate compound used, and age, weight and response of the individual patients.

[0161] The amount of zoledronic acid or another bisphosphonate in a therapeutic composition may vary. For example, some liquid compositions may comprise about 0.0001% (w/v) to about 50% (w/v), about 0.01% (w/v) to about 20%
(w/v), about 0.01% to about 10% (w/v), about 0.001% (w/v) to about 1% (w/v), about 0.1% (w/v) to about 0.5% (w/v), about 1% (w/v) to about 3% (w/v), about 3%
(w/v) to Date Recue/Date Received 2021-04-07 about 5% (w/v), about 5% (w/v) to about 7% (w/v), about 7% (w/v) to about 10%
(w/v), about 10% (w/v) to about 15% (w/v), about 15% (w/v) to about 20% (w/v), about 20% (w/v) to about 30% (w/v), about 30% (w/v) to about 40% (w/v), or about 40% (w/v) to about 50% (w/v) of zoledronic acid.

[0162] Some solid compositions may comprise at least about 5% (w/w), at least about 10% (w/w), at least about 20% (w/w), at least about 50% (w/w), at least about 70% (w/w), at least about 80%, about 10% (w/w) to about 30% (w/w), about 10% (w/w) to about 20% (w/w), about 20% (w/w) to about 30% (w/w), about 30%
(w/w) to about 50% (w/w), about 30% (w/w) to about 40% (w/w), about 40% (w/w) to about 50% (w/w), about 50% (w/w) to about 80% (w/w), about 50% (w/w) to about 60% (w/w), about 70% (w/w) to about 75% (w/w), about 70% (w/w) to about 80%
(w/w), or about 80% (w/w) to about 90% (w/w) of zoledronic acid.

[0163] Any suitable amount of an osteoclast inhibitor, including a bisphosphonate, such as a nitrogen-containing bisphosphonate, e.g. zoledronic acid, minodronic acid, or ibandronic acid, may be used. Some solid or liquid oral dosage forms, or units of oral dosage forms (referred to collectively herein as "oral dosage form(s)") may contain about 0.005 mg to about 20 mg, about 0.1 mg to about 10 mg, about 0.5 mg to about 10 mg, about 0.2 mg to about 5 mg, about 1 mg to about mg, about 1 mg to about 50 mg, about 10 mg to about 250 mg, about 100 mg to about 300 mg, about 20 mg to about 200 mg, about 20 mg to about 150 mg, about 30 mg to about 100 mg, about 1 mg to about 1,000 mg, about 10 mg to about 50 mg, about 40 mg to about 60 mg, about 50 mg to about 60 mg, about 55 mg, about 10 mg to about 300 mg, about 10 mg to about 150 mg, about 10 mg to about 100 mg, about 40 mg to about 150 mg, about 10 mg to about 600 mg, about 40 mg to about 600 mg, about 40 mg to about 2000 mg, about 40 mg to about 800 mg, about 25 mg to about 800 mg, about 30 mg to about 800 mg, about 10 mg to about 500 mg, about 50 mg to about 150 mg, about 50 mg, about 100 mg, about 50 mg to about 500 mg, about 100 mg to about 2000 mg, about 300 mg to about 1500 mg, about 200 mg to about 1000 mg, about 100 mg to about 500 mg, or about 150 mg of zoledronic acid, or any amount of osteoclast inhibitor in a range bounded by, or between, any of these values. In some embodiments, the oral osteoclast inhibitor is administered daily, weekly, monthly, every two or three months, once a year, or twice a year.

Date Recue/Date Received 2021-04-07

[0164] Some oral dosage forms may contain about 0.005 mg to about 20 mg, about 0.1 mg to about 10 mg, about 0.5 mg to about 10 mg, about 0.2 mg to about 5 mg, about 1 mg to about 500 mg, about 1 mg to about 50 mg, about 10 mg to about 250 mg, about 100 mg to about 300 mg, about 20 mg to about 200 mg, about 20 mg to about 150 mg, about 30 mg to about 100 mg, about 1 mg to about 1,000 mg, about 10 mg to about 50 mg, about 40 mg to about 60 mg, about 50 mg to about 60 mg, about 55 mg, about 10 mg to about 300 mg, about 10 mg to about mg, about 10 mg to about 100 mg, about 40 mg to about 150 mg, about 10 mg to about 600 mg, about 40 mg to about 600 mg, about 40 mg to about 2000 mg, about 40 mg to about 800 mg, about 25 mg to about 800 mg, about 30 mg to about 800 mg, about 10 mg to about 500 mg, about 50 mg to about 150 mg, about 50 mg, about 100 mg, about 50 mg to about 500 mg, about 100 mg to about 2000 mg, about 300 mg to about 1500 mg, about 200 mg to about 1000 mg, about 100 mg to about 500 mg, or about 150 mg of osteoclast inhibitor, or any amount of osteoclast inhibitor in a range bounded by, or between, any of these values. In some embodiments, the oral osteoclast inhibitor is administered daily, weekly, monthly, every two or three months, once a year, or twice a year.

[0165] In some embodiments, an oral dosage form may contain about 10 mg/m2 to about 20 mg/m2, about 15 mg/m2 to about 20 mg/m2, about 18 mg/m2, about 80 mg/m2 to about 150 mg/m2, about 90 mg/m2 to about 150 mg/m2, about 100 mg/m2 to about 150 mg/m2 of zoledronic acid, or any amount of zoledronic in a range bounded by, or between, any of these values. All dosage ranges or amounts expressed in mg/m2 are based upon the body surface area of the mammal.

[0166] In some embodiments, the daily oral dose of an osteoclast inhibitor, including a bisphosphonate, such as a nitrogen-containing bisphosphonate, e.g.

zoledronic acid, minodronic acid, or ibandronic acid, is about 0.005 mg to about 20 mg, about 0.1 mg to about 10 mg, about 0.5 mg to about 10 fig, about 0.2 mg to about 5 mg, or any amount in a range bounded by, or between, any of these values.
In some embodiments, the daily oral dose of osteoclast inhibitor is less than about 35 mg/m2, less than about 30 mg/m2, less than about 25 mg/m2, about 1 mg/m2 to about 35 mg/m2, about 1 mg/m2 to about 30 mg/m2, about 1.5 mg/m2 to about 25 mg/m2, about 1.8 mg/m2 to about 20 mg/m2, about 10 mg/m2 to about 20 mg/m2, about 10 mg/m2 to about 30 mg/m2, about 15 mg/m2 to about 20 mg/m2, about 18 Date Recue/Date Received 2021-04-07 mg/m2, or any amount of zoledronic acid in a range bounded by, or between, any of these values.

[0167] In some embodiments, the daily oral dose of an osteoclast inhibitor, including a bisphosphonate, such as a nitrogen-containing bisphosphonate, e.g.

zoledronic acid, minodronic acid, or ibandronic acid, is about 0.005 mg to about 20 mg, about 0.1 mg to about 10 mg, about 0.5 mg to about 10 mg, about 0.2 mg to about 5 mg, or any amount of osteoclast inhibitor in a range bounded by, or between, any of these values. In some embodiments, the daily oral dose of osteoclast inhibitor is less than about 35 mg/m2, less than about 30 mg/m2, less than about 25 mg/m2, about 1 mg/m2 to about 35 mg/m2, about 1 mg/m2 to about 30 mg/m2, about 1,5 mg/rn2 to about 25 mg/m2, about 1.8 mg/m2 to about 20 mg/m2, about 10 mg/rn2 to about 20 mg/m2, about 10 mg/m2 to about 30 mg/m2, about 15 mg/m2 to about 20 mg/rn2, about 18 mg/rn2, or any amount of osteoclast inhibitor in a range bounded by, or between, any of these values.

[0168] In some embodiments the daily oral dose of zoledronic acid is about 0.005 mg to about 20 mg, about 0.1 mg to about 10 mg, about 0.5 mg to about 10 mg, about 0.2 mg to about 5 mg, or any amount of zoledronic acid in a range bounded by, or between, any of these values. In some embodiments, the daily oral dose of zoledronic acid is less than about 35 mg/m2, less than about 30 mg/rn2, less than about 25 mg/m2, about 1 mg/m2 to about 35 mg/rn2, about 1 mg/rn2 to about mg/m2, about 1.5 mg/m2 to about 25 mg/m2, about 1,8 mg/m2 to about 20 mg/m2, about 10 mg/m2 to about 20 mg/m2, about 10 mg/m2 to about 30 mg/m2, about 15 mg/m2 to about 20 mg/rn2, about 18 mg/m2, or any amount of zoledronic acid in a range bounded by, or between, any of these values.

[0169] In some embodiments, the weekly oral dose of the osteoclast inhibitor, including a bisphosphonate, such as a nitrogen-containing bisphosphonate, e.g. zoledronic acid, minodronic acid, or ibandronic acid, is about 1 mg to about 1000 mg, about 1 mg to about 500 mg, about 10 mg to about 250 mg, about 100 mg to about 300 mg, about 10 mg to about 100 mg, about 10 mg to about 150 mg, about mg to about 100 mg, about 10 mg to about 300 mg, about 20 mg to about 150 mg, about 20 mg to about 60 mg, about 30 mg to about 70 mg, about 40 mg to about 60 mg, about 50 mg to about 70 mg, about 50 mg, about 55 mg, about 100 mg to about 150 mg, or about 30 mg to about 100 mg. in some embodiments, the weeky Date Recue/Date Received 2021-04-07 oral dose of the osteoclast inhibitor is less than about 250 mg/m2, less than about 200 mg/rn2, less than about 175 mg/m2, about 6 mg/m2 to about 250 mg/m2, about mg/m2 to about 210 mg/1-W, about 10 rna/m2 to about 170 mg/m2, about 4 mg/m2 to about 140 mg/rn2, about 100 rng/m2 to about 140 mg/rn2, about 126 mg/m2, or any amount in a range bounded by, or between, any of these values. The weekly oral dose may be given as a single dose, given once during the week, or may be given in 2, 3, 4, 5, 6, or 7 individual doses during the week.

[0170] In some embodiments the weekly oral dose of zoledronic acid is about 1 mg to about 1000 mg, about 1 mg to about 500 mg, about 10 mg to about 250 mg, about 100 mg to about 300 mg, about 10 mg to about 100 mg, about 10 mg to about 150 mg, about 10 mg to about 100 mg, about 10 mg to about 300 mg, about mg to about 150 mg, about 20 mg to about 60 mg, about 30 mg to about 70 mg, about 40 mg to about 60 rng, about 50 mg to about 70 mg, about 50 mg, about 55 mg, about 100 mg to about 150 mg, or about 30 mg to about 100 mg. In some embodiments, the weeky oral dose of zoledronic acid is less than about 250 mg/m2, less than about 200 mg/rn2, less than about 175 mg/m2, about 6 mg/m2 to about ma/m2, about 10 mg/m2 to about 210 mg/m2, about 10 mg/m2 to about 170 mg/m2, about 4 mg/m2 to about 140 mg/m2, about 100 mg/m2 to about 140 mg/rn2, about 126 mg/m2, or any amount of zoledronic acid in a range bounded by, or between, any of these values.
The weekly oral dose may be given as a single dose, given once during the week, or may be given in 2, 3, 4, 5, 6, or 7 individual doses during the week.

[0171] In some embodiments, the monthly dose of the osteoclast inhibitor, including a bisphosphonate, such as a nitrogen-containing bisphosphonate, e.g.

zoledronic acid, minodronic acid, or ibandronic acid, or the amount of the osteoclast inhibitor that is administered over a period of a month, is about 5000 mg or less, about 4000 mg or less, about 3000 mg or less, about 2000 mg or less, about mg or less, about 700 mg or less, about 600 mg or less, about 1 mg to about 4,000 mg, about 1 mg to about 1,000 mg, about 10 mg to about 1000 mg, about 50 mg to about 1000 mg, about 10 mg to about 600 mg, about 40 mg to about 600 rng, about 50 mg to about 600 mg, about 40 mg to about 400 mg, about 50 rng to about 200 mg, about 200 mg to about 300 mg, about 250 mg to about 350 mg, or about 100 mg to about 600 mg, about 40 mg to about 2000 mg, about 40 mg to about 800 mg, Date Recue/Date Received 2021-04-07 about 50 mg to about 800 mg, or about 100 mg to about 800 mg, about 40 mg to about 1000 mg, about 50 mg to about 1000 mg, or about 100 mg to about 1000 mg, or any monthly dose in a range bounded by, or between, any of these values. In some embodiments, the monthly oral dose of the osteoclast inhibitor is less than about 1000 mg/m2, less than about 800 mg/m2, less than about 600 mg/m2, about mg/m2 to about 1000 mg/m2, about 50 mg/m2 to about 800 mg/m2, about 70 mg/m2 to about 700 mg/m2, about 100 mg/m2 to about 700 mg/m2, about 100 mg/m2 to about 600 mg/m2, about 50 mg/m2 to about 200 mg/m2, about 300 mg/m2 to about 600 mg/m2, about 450 mg/m2 to about 600 mg/m2, about 300 mg/m2 to about 1000 mg/m2, about 400 mg/m2 to about 1000 mg/m2, about 500 mg/m2 to about 1000 mg/m2, about 400 mg/m2 to about 700 mg/m2, about 500 mg/m2 to about 600 mg/m2, about 540 mg/m2, or any amount in a range bounded by, or between, any of these values. A monthly dose may be given as a single dose, or as two or more individual doses administered during the month. In some embodiments, the monthly dose is administered in 2 or 3 weekly doses. In some embodiments, the monthly dose is administered in 4 or 5 weekly doses. In some embodiments, the monthly dose is administered in 28 to 31 daily doses. In some embodiments, the monthly dose is administered in 5 to 10 individual doses during the month. The monthly dose may be administered for only 1 month, or may be repeatedly administered for 2 or more months.

[0172] In some embodiments, the monthly dose of zoledronic acid, or the amount of zoledronic acid that is administered over a period of a month, is about 5000 mg or less, about 4000 mg or less, about 3000 mg or less, about 2000 mg or less, about 1000 mg or less, about 700 mg or less, about 600 mg or less, about 1 mg to about 4,000 mg, about 1 mg to about 1,000 mg, about 10 mg to about 1000 mg, about 50 mg to about 1000 mg, about 10 mg to about 600 mg, about 40 mg to about 600 mg, about 50 mg to about 600 mg, about 40 mg to about 400 mg, about 50 mg to about 200 mg, about 200 mg to about 300 mg, about 250 mg to about 350 mg, or about 100 mg to about 600 mg, about 40 mg to about 2000 mg, about 40 mg to about 800 mg, about 50 mg to about 800 rng, or about 100 mg to about 800 mg, about 40 mg to about 1000 mg, about 50 mg to about 1000 mg, or about 100 mg to about 1000 mg, or any monthly dose in a range bounded by, or between, any of these values. In some embodiments, the monthly oral dose of zoledronic acid is less than about 1000 mg/m2, less than about 800 mg/m2, less than about 600 mg/m2, Date Recue/Date Received 2021-04-07 about 10 mg/m2 to about 1000 mg/m2, about 50 mg/m2 to about 800 mg/m2, about 70 mg/m2 to about 700 mg/m2, about 100 mg/m2 to about 700 mg/m2, about 100 mg/m2 to about 600 mg/m2, about 50 mg/m2 to about 200 mg/m2, about 300 mg/m2 to about 600 mg/m2, about 450 mg/m2 to about 600 mg/m2, about 300 mg/m2 to about 1000 mg/m2, about 400 mg/m2 to about 1000 mg/m2, about 500 mg/m2 to about 1000 mg/m2, about 400 mg/m2 to about 700 mg/m2, about 500 mg/m2 to about 600 mg/m2, about 540 mg/m2, or any amount of zoledronic acid in a range bounded by, or between, any of these values. A monthly dose may be given as a single dose, or as two or more individual doses administered during the month. In some embodiments, the monthly dose is administered in 2 or 3 weekly doses. In some embodiments, the monthly dose is administered in 4 or 5 weekly doses. In some embodiments, the monthly dose is administered in 28 to 31 daily doses. In some embodiments, the monthly dose is administered in 5 to 10 individual doses during the month. The monthly dose may be administered for only 1 month, or may be repeatedly administered for 2 or more months.

[0173] With respect to orally administering zoledronic acid to a mammal, such as a dog, a rat, a rabbit, a monkey, an ape, or a human being, doses of about 0.03 mg/kg to about 10 mg/kg, or any smaller range within this range, such as about 0.4 mg/kg to about 3 mg/kg, about 0.4 mg/kg to about 1.5 mg/kg, mg/kg, about 0.4 mg/kg to about 0.5 mg/kg, about 0.5 mg/kg to about 0.6 mg/kg, about 0.6 mg/kg to about 0.7 mg/kg, about 0.7 mg/kg to about 0.8 mg/kg, about 0.8 mg/kg to about 0.9 mg/kg, about 0.9 mg/kg to about 1 mg/kg, about 1 mg/kg to about 1.1 mg/kg, about 1.1 mg/kg to about 1.2 mg/kg, about 1.2 mg/kg to about 1.3 mg/kg, about 1.3 mg/kg to about 1.4 mg/kg, about 1.4 mg/kg to about 1.5 mg/kg, about 1.5 mg/kg to about 1.6 mg/kg, about 1.6 mg/kg to about 1.7 mg/kg, about 1.7 mg/kg to about 1.8 mg/kg, about 1.8 mg/kg to about 1.9 mg/kg, about 1.9 mg/kg to about 2 mg/kg, about 2 mg/kg to about 2.1 mg/kg, about 2.1 mg/kg to about 2.2 mg/kg, about 2.2 mg/kg to about 2.3 mg/kg, about 2.3 mg/kg to about 2.4 mg/kg, about 2.4 mg/kg to about 2.5 mg/kg, about 2.5 mg/kg to about 2.6 mg/kg, about 2.6 mg/kg to about 2.7 mg/kg, about 2.7 mg/kg to about 2.8 mg/kg, about 2.8 mg/kg to about 2.9 mg/kg, about 2.9 mg/kg to about 3 mg/kg, about 3 mg/kg to about 3.1 mg/kg, about 3.1 mg/kg to about 3.2 mg/kg, about 3.2 mg/kg to about 3.3 mg/kg, about 3.3 mg/kg to about 3.4 mg/kg, about 3.4 mg/kg to about 3.5 mg/kg, about 3.5 mg/kg to about 3.6 mg/kg, about 3.6 mg/kg to about 3.7 mg/kg, about 3.7 mg/kg to about 3.8 mg/kg, about 3.8 mg/kg to Date Recue/Date Received 2021-04-07 about 3.9 mg/kg, about 3.9 mg/kg to about 4 mg/kg, about 0.4 mg/kg to about 0.6 mg/kg, about 0.6 mg/kg to about 0.8 ring/kg, about 0.8 mg/kg to about 1 mg/kg, about 1 mg/kg to about 1.2 mg/kg, about 1.2 mg/kg to about 1.4 mg/kg, about 1.4 mg/kg to about 1.6 mg/kg, about 1.6 mg/kg to about 1.8 mg/kg, about 1.8 mg/kg to about mg/kg, about 2 mg/kg to about 2.2 mg/kg, about 2.2 mg/kg to about 2.4 mg/kg, about 2.4 mg/kg to about 2.6 mg/kg, about 2.6 mg/kg to about 2.8 mg/kg, about 2.8 mg/kg to about 3 mg/kg, about 3 mg/kg to about 3.2 mg/kg, about 3.2 mg/kg to about 3.4 mg/kg, about 3.4 mg/kg to about 3.6 mg/kg, about 3.6 mg/kg to about 3.8 mg/kg, about 3.8 mg/kg to about 4 mg/kg, about 0.4 mg/kg to about 0.7 mg/kg, about 0.7 mg/kg to about 1 mg/kg, about 1 mg/kg to about 1.3 mg/kg, about 1.3 mg/kg to about 1.6 mg/kg, about 1.6 mg/kg to about 1.9 mg/kg, about 1.9 mg/kg to about 2.2 mg/kg, about 2.2 mg/kg to about 2.5 mg/kg, about 2.5 mg/kg to about 2.8 mg/kg, about 2.8 mg/kg to about 3 mg/kg, about 3.3 mg/kg to about 3.6 mg/kg, about 3.6 mg/kg to about 4 mg/kg, about 0.4 mg/kg to about 1 mg/kg, or about 0.5 mg/kg to about 1 mg/kg, may be a safe dose for repeated oral administration, such as once daily dosing to once yearly dosing, once daily dosing to twice yearly dosing, once daily dosing to thrice yearly dosing, once daily dosing to dosing every three months, once daily dosing to dosing every two months, once daily dosing to dosing every two months, once daily dosing to dosing every month, once daily dosing to dosing every 2-4 weeks, once daily dosing to once weekly dosing, etc.

[0174] The doses referred to in the paragraph above for administration of zoledronic acid to a mammal may be safely administered 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, or 15 times, or about 3 to about 10 times, once a day, or less frequently, such as once week, once every two weeks, once a month, etc.

[0175] For once daily to once weekly oral administration of zoledronic acid to a mammal such as a mouse, rat, dog, primate, or a human being, in some embodiments, a safely repeated dose may be about 0.03 mg/kg to about 4 mg/kg, or any smaller range within this range, such as about 0.01 mg/kg to about 0.02 mg/kg, about 0.02 mg/kg to about 0.03 mg/kg, about 0.03 mg/kg to about 0.04 mg/kg, about 0.04 mg/kg to about 0.05 mg/kg, about 0.05 mg/kg to about 0.06 mg/kg, about 0.06 mg/kg to about 0.07 mg/kg, about 0.07 mg/kg to about 0.08 mg/kg, about 0.08 mg/kg to about 0.09 mg/kg, about 0.09 mg/kg to about 0.1 mg/kg, about 0.1 mg/kg to about 0.11 mg/kg, about 0.11 mg/kg to about 0.12 mg/kg, about 0.12 mg/kg to about 0.13 mg/kg, about 0.13 mg/kg to about 0.14 mg/kg, about 0.14 mg/kg to about 0.15 Date Recue/Date Received 2021-04-07 mg/kg, about 0.15 mg/kg to about 0.16 mg/kg, about 0.16 mg/kg to about 0.17 mg/kg, about 0.17 mg/kg to about 0.18 mg/kg, about 0.18 mg/kg to about 0.19 mg/kg, about 0.19 mg/kg to about 0.2 mg/kg, about 0.2 mg/kg to about 0.21 mg/kg, about 0.21 mg/kg to about 0.22 mg/kg, about 0.22 mg/kg to about 0.23 mg/kg, about 0.23 mg/kg to about 0.24 mg/kg, about 0.24 mg/kg to about 0.25 mg/kg, about 0.25 mg/kg to about 0.26 mg/kg, about 0.26 mg/kg to about 0.27 mg/kg, about 0.27 mg/kg to about 0.28 mg/kg, about 0.28 mg/kg to about 0.29 mg/kg, about 0.29 mg/kg to about 0.3 mg/kg, about 0.3 mg/kg to about 0.31 mg/kg, about 0.31 mg/kg to about 0.32 mg/kg, about 0.32 mg/kg to about 0.33 mg/kg, about 0.33 mg/kg to about 0.34 mg/kg, about 0.34 mg/kg to about 0.35 mg/kg, about 0.35 mg/kg to about 0.36 mg/kg, about 0.36 mg/kg to about 0.37 mg/kg, about 0.37 mg/kg to about 0.38 mg/kg, about 0.38 mg/kg to about 0.39 mg/kg, about 0.39 mg/kg to about 0.4 mg/kg, about 0.05 mg/kg to about 0.2 mg/kg, about 0.05 mg/kg to about 0.15 mg/kg, about 0.06 mg/kg to about 0.15 mg/kg, about 0.07 mg/kg to about 0.15 mg/kg, about 0.08 mg/kg to about 0.15 mg/kg, about 0.09 mg/kg to about 0.15 mg/kg, about 0.1 mg/kg to about 0.15 mg/kg, about 0.03 mg/kg to about 0.5 mg/kg, about 0.06 mg/kg to about 0.2 mg/kg, about 0.07 mg/kg to about 0.2 mg/kg, about 0.08 mg/kg to about 0.2 mg/kg, about 0.09 mg/kg to about 0.2 mg/kg, about 0.1 mg/kg to about 0.2 mg/kg, about 0.4 mg to about 4 mg, about 0.4 mg/kg to about 0.6 mg/kg, about 0.6 mg/kg to about 0.8 mg/kg, about 0.8 mg/kg to about 1 mg/kg, about 1 mg/kg to about 1.2 mg/kg, about 1.2 mg/kg to about 1.4 mg/kg, about 1.4 mg/kg to about 1.6 mg/kg, about 1.6 mg/kg to about 1.8 mg/kg, about 1.8 mg/kg to about 2 mg/kg, about 2 mg/kg to about 2.2 mg/kg, about 2.2 mg/kg to about 2.4 mg/kg, about 2.4 mg/kg to about 2.6 mg/kg, about 2.6 mg/kg to about 2.8 mg/kg, about 2.8 mg/kg to about mg/kg, about 3 mg/kg to about 3.2 mg/kg, about 3.2 mg/kg to about 3.4 mg/kg, about 3.4 mg/kg to about 3.6 mg/kg, about 3.6 mg/kg to about 3.8 mg/kg, about 3.8 mg/kg to about 4 mg/kg, about 0.5 mg/kg to about 2 mg/kg, about 0.6 mg/kg to about 2 mg/kg, about 0.7 mg/kg to about 2 mg/kg, about 0.8 mg/kg to about 2 mg/kg, about 0.5 mg/kg to about 1.5 mg/kg, about 0.6 mg/kg to about 1.5 mg/kg, about 0.7 mg/kg to about 1.5 mg/kg, about 0.8 mg/kg to about 1.5 mg/kg, about 0.5 mg/kg to about 0.9 mg/kg, about 0.6 mg/kg to about 0.9 mg/kg, about 0.7 mg/kg to about 0.9 mg/kg, about 0.5 mg/kg to about 1 mg/kg, about 0.6 mg/kg to about 1 mg/kg, about 0.7 mg/kg to about 1 mg/kg, about 0.8 mg/kg to about 1 mg/kg, or about 0.8 mg/kg to about 0.9 mg/kg.

Date Recue/Date Received 2021-04-07

[0176] For once weekly or less frequent oral administration of zoledronic acid to a mammal such as a mouse, rat, dog, primate, or a human being, in some embodiments, a safely repeated dose may be about 0.4 mg to about 10 mg, or any smaller range within this range, such as about 0.4 mg/kg to about 0.6 mg/kg, about 0.6 mg/kg to about 0.8 mg/kg, about 0.8 mg/kg to about 1 mg/kg, about 1 mg/kg to about 1.2 mg/kg, about 1.2 mg/kg to about 1.4 mg/kg, about 1.4 mg/kg to about 1.6 mg/kg, about 1.6 mg/kg to about 1.8 mg/kg, about 1.8 mg/kg to about 2 mg/kg, about 2 mg/kg to about 2.2 mg/kg, about 2.2 mg/kg to about 2.4 mg/kg, about 2.4 mg/kg to about 2.6 mg/kg, about 2.6 mg/kg to about 2.8 mg/kg, about 2.8 mg/kg to about mg/kg, about 3 mg/kg to about 3.2 mg/kg, about 3.2 mg/kg to about 3.4 mg/kg, about 3.4 mg/kg to about 3.6 mg/kg, about 3.6 mg/kg to about 3.8 mg/kg, about 3.8 mg/kg to about 4 mg/kg, about 4 mg/kg to about 4.2 mg/kg, about 4.2 mg/kg to about 4.4 mg/kg, about 4.4 mg/kg to about 4.6 mg/kg, about 4.6 mg/kg to about 4.8 mg/kg, about 4.8 mg/kg to about 5 mg/kg, about 5 mg/kg to about 5.2 mg/kg, about 5.2 mg/kg to about 5.4 mg/kg, about 5.4 mg/kg to about 5.6 mg/kg, about 5.6 mg/kg to about 5.8 mg/kg, about 5.8 mg/kg to about 6 mg/kg, about 6 mg/kg to about 6.2 mg/kg, about 6.2 mg/kg to about 6.4 mg/kg, about 6.4 mg/kg to about 6.6 mg/kg, about 6.6 mg/kg to about 6.8 mg/kg, about 6.8 mg/kg to about 7 mg/kg, about 7 mg/kg to about 7.2 mg/kg, about 7.2 mg/kg to about 7.4 mg/kg, about 7.4 mg/kg to about 7.6 mg/kg, about 7.6 mg/kg to about 7.8 mg/kg, about 7.8 mg/kg to about mg/kg, about 8 mg/kg to about 8.2 mg/kg, about 8.2 mg/kg to about 8.4 mg/kg, about 8.4 mg/kg to about 8.6 mg/kg, about 8.6 mg/kg to about 8.8 mg/kg, about 8.8 mg/kg to about 9 mg/kg, about 9 mg/kg to about 9.2 mg/kg, about 9.2 mg/kg to about 9.4 mg/kg, about 9.4 mg/kg to about 9.6 mg/kg, about 9.6 mg/kg to about 9.8 mg/kg, about 9.8 mg/kg to about 10 mg/kg, about 0.5 mg/kg to about 2 mg/kg, about 0.6 mg/kg to about 2 mg/kg, about 0.7 mg/kg to about 2 mg/kg, about 0.8 mg/kg to about 2 mg/kg, about 0.5 mg/kg to about 1.5 mg/kg, about 0.6 mg/kg to about 1.5 mg/kg, about 0.7 mg/kg to about 1.5 mg/kg, about 0.8 mg/kg to about 1.5 mg/kg, about 0.5 mg/kg to about 1 mg/kg, about 0.6 mg/kg to about 1 mg/kg, about 0.7 mg/kg to about 1 mg/kg, about 0.8 mg/kg to about 1 mg/kg, or about 0.8 mg/kg to about 0.9 mg/kg,

[0177] In some embodiments, the osteoclast inhibitor comprises zoledronic acid, and the oral zoledronic acid, or disodium salt thereof, may be administered in combination with about 0.1 mg to about 10 mg of zoledronic acid, or a salt thereof, Date Recue/Date Received 2021-04-07 administered parenterally, such as intravenously. In some embodiments, about mg, about 100 mg, or about 150 mg of the disodium salt of zoledronic acid is administered orally in combination with 1 mg parenteral, such as intravenous, zoledronic acid. In some embodiments the parenterai dose of zoledronic acid is about 0.25 mg to about 25 mg, about 0.25 mg to about 10 mg, or about 0.5 mg to about 7.5 mg.

[0178] With respect to oral administration of an osteoclast inhibitor, such as zoledronic acid, minodronic acid, ibandronic acid, or another bisphosphonate, for the treatment of pain associated with inflammation, arthritis, CRPS, or any other condition recited herein, it may helpful if the mammal or human being to which the osteoclast inhibitor is administered does not eat food or drink beverage, (other than any water required to swallow the oral dosage form) for at least about 1 hour, at least about 2 hours, at least about 4 hours, at least about 6 hours, at least about 8 hours, at least about 10 hours, or at least about 12 hours before the osteoclast inhibitor is administered. It may also be helpful if the mammal or human being to which the osteoclast inhibitor is administered does not eat food or drink beverage for at least about 30 minutes, at least about 1 hour, at least about 2 hours, at least about 3 hours, or at least about 4 hours after the osteoclast inhibitor is administered. In some embodiments, a human being to which the zoledronic acid is administered avoids lying down, or remains upright or sits upright, for at least about 30 minutes or about 1 hour after receiving a dosage form containing the osteoclast inhibitor.
Avoiding food or beverage before or after oral administration of the osteoclast inhibitor can improve the bioavailability of the osteoclast inhibitor.

[0179] The oral bioavailability of osteoclast inhibitor in a dosage form can vary. Some dosage forms may have ingredients added to enhance the bioavailability. However, bioavailability enhancement is not necessary for an oral dosage form to be effective. In some embodiments, the dosage form is substantially free of bioavailability-enhancing agents. In some embodiments, an oral dosage form may have an oral bioavailability of the osteoclast inhibitor¨such as zoledronic acid, minodronic acid, ibandronic acid¨of about 0.01% to about 10%, about 0.1% to about 7%, about 0.1% to about 5%, etc. Without ingredients or other methods to enhance bioavailability, bisphosphonates such as zoledronic acid typically have a low bioavailability in an oral dosage form. In some embodiments, the oral Date Recue/Date Received 2021-04-07 bioavailability of zoledronic acid is unenhanced or substantially unenhanced.
For example, the oral bioavailability of zoledronic acid can be about 0.01% to about 5%, about 0.01% to about 4%, about 0.1% to about 3%, about 0.1% to about 2%, about 0.2% to about 2%, about 0.2% to about 1.5%, about 0.3% to about 1.5%, about 0.3% to about 1%, about 1% to about 3%, about 1.2% to about 3.5%, about 1.2%
to about 3%, about 1% to about 4%, about 1.5% to about 4.5%, about 0.1% to about 0.5%, about 0.3% to about 0.5%, about 0.5% to about 1%, about 0.6% to about 0.7%, about 0.7% to about 0.8%, about 0.8% to about 0.9%, about 0.9%, about 1%

to about 1.1%, about 1.1% to about 1.2%, about 1.2% to about 1.3%, about 1.3%
to about 1.4%, about 1.4% to about 1.5%, about 1.5% to about 1.6%, about 1.6% to about 1.8%, about 1.8% to about 2%, or about 2% to 2.5%.

[0180] One embodiment is a pharmaceutical composition comprising an osteoclast inhibitor such as zoledronic acid, minodronic acid, or ibandronic acid wherein the oral bloavallability of zoledronic acid in the dosage form is from about 0.01% to about 10%.

[0181] In some embodiments, the oral bioavailability of the osteoclast inhibitor in the dosage form is about 0.01% to about 5%, about 0.1% to about 7%, about 0.1% to about 5%, about 0.1% to about 3%, about 0.1% to about 2%, about 0.2% to about 2%, about 0.2% to about 1.5%, about 0.3% to about 1.5%, or about 0.3% to about 1.0%.

[0182] In some embodiments, the oral bioavailability of zoledronic acid in the dosage form is about 0.01% to about 5%.

[0183] In some embodiments, the oral bioavailability of zoledronic acid in the dosage form is about 0.1% to about 7%.

[0184] In some embodiments, the oral bioavailability of zoledronic acid in the dosage form is about 0.1% to about 5%.

[0185] In some embodiments, the oral bioavailability of zoledronic acid in the dosage form is about 0.1% to about 3%.

[0186] In some embodiments, the oral bioavailability of zoledronic acid in the dosage form is about 0.1% to about 2%.

Date Recue/Date Received 2021-04-07

[0187] In some embodiments, the or bioavailability of zoledronic acid in the dosage form is about 0.2% to about 2%.

[0188] In some embodiments, the oral bioavailability of zoledronic acid in the dosage form is about 0.2% to about 1.5%.

[0189] In some embodiments, the or bioavailability of zoledronic acid in the dosage-) form is about 0.3% to about 1.5%.

[0190] In some embodiments, the oral bioavaiiability of zoledronic acid in the dosage form is about 0.3% to about 1.0%.

[0191] In some embodiments, an oral dosage form comprises about 10 mg to about 300 mg of zoledronic acid, minodronic acid, or ibandronic acid and is administered daily for about 2 to about 15 consecutive days. This regimen may be repeated once monthly, once every two months, once every three months, once every four months, once every five months, once every six months, once yearly, or once every two years.

[0192] In some embodiments, an oral dosage form comprises about 10 mg to about 150 mg or about 10 mg to about 100 mg of zoledronic acid, minodronic acid, or ibandronic acid and is administered daily for about 2 to about 15 consecutive days. This regimen may be repeated once monthly, once every two months, once every three months, once every four months, once every five months, once every six months, once yearly, or once every two years.

[0193] In some embodiments, an oral dosage form comprises about 10 mg to about 150 mg or about 10 mg to about 100 mg of zoledronic acid, minodronic acid, or ibandronic acid and is administered daily for about 5 to about 10 consecutive days. This regimen may be repeated once monthly, once every two months, once every three months, once every four months, once every five months, once every six months, once yearly, or once every two years.

[0194] In some embodiments, an oral dosage form comprises about 40 frICI
to about. 150 mg of zoledronic acid, minodronic acid, or ibandronic acid and is administered daily for about 5 to about 10 consecutive days. This regimen may be repeated once monthly, once every two months, once every three months, once every four months, once every five months, once every six months, once yearly, or once every two years.
so Date Recue/Date Received 2021-04-07

[0195] In some embodiments, the oral zoledronic acid, minodronic acid, or ibandronic acid may be administered as one dose of about 100 mg to about 2000 ma. In some embodiments, the oral zoledronic acid, minodronic acid, or ibandronic acid may be administered as one dose of about 300 mg to about 1500 mg. In some embodiments, the oral zoledronic acid, minodronic acid, or ibandronic acid may be administered as one dose of about 200 mg to about 1000 mg, The dose of zoledronic acid, minodronic acid, or ibandronic acid may be administered in a single or divided dose.

[0196] An osteoclast inhibitor, such as zoledronic acid, minodronic acid, or ibandronic acid, may be formulated for oral administration, for example, with an inert diluent or with an edible carrier, or it may be enclosed in hard or soft shell gelatin capsules, compressed into tablets, or incorporated directly with the food of the diet.
For oral therapeutic administration, the active compound may be incorporated with an excipient and used in the form of ingestible tablets, buccal tablets, coated tablets, troches, capsules, elixirs, dispersions, suspensions, solutions, syrups, wafers, patches, and the like.

[0197] Tablets, troches, pills, capsules and the like may also contain one or more of the following: a binder such as gum tragacanth, acacia, corn starch or gelatin; an excipient, such as dicalcium phosphate; a disintegrating agent such as corn starch, potato starch, alginic acid and the like; a lubricant such as magnesium stearate; a sweetening agent such as sucrose, lactose or saccharin; or a flavoring agent such as peppermint, oil of wintergreen or cherry flavoring. When the unit dosage form is a capsule, it may contain, in addition to materials of the above type, a liquid carrier. Various other materials may be present as coating, for instance, tablets, pills, or capsules may be coated with shellac, sugar or both. A syrup or elixir may contain the active compound, sucrose as a sweetening agent, methyl and propylparabens as preservatives, a dye and flavoring, such as cherry or orange flavor. It may be desirable for material in a dosage form or pharmaceutical composition to be pharmaceutically pure and substantially non toxic in the amounts employed.

[0198] Some compositions or dosage forms may be a liquid, or may comprise a solid phase dispersed in a liquid.

Date Recue/Date Received 2021-04-07

[0199] An osteoclast inhibitor, such as zoledronic acid, minodronic acid, or ibandronic acid may be formulated for parental or intraperitoneal administration.
Solutions of the active compounds as free acids or pharmacologically acceptable salts can be prepared in water suitably mixed with a surfactant, such as hydroxypropylcellulose. A dispersion can also have an oil dispersed within, or dispersed in, glycerol, liquid polyethylene glycols, and mixtures thereof.
Under ordinary conditions of storage and use, these preparations may contain a preservative to prevent the growth of microorganisms.

[0200] In some embodiments, an oral dosage form may comprise a TM
silicified microcrystalline cellulose such as Prosolv. For example, about 20%
(wt/wt) to about 70% (wt/wt), about 10% (wt/wt) to about 20% (wt/wt), about 20%
(wt/wt) to about 40% (wt/wt), about 25% (wt/wt) to about 30% (wt/wt), about 40% (wt/wt) to about 50% (wt/wt), or about 45% (wt/wt) to about 50% (wt/wt) silicified microcrystalline cellulose may be present in an oral dosage form or a unit of an oral dosage form.

[0201] In some embodiments, an oral dosage form may comprise a crosslinked polyvinylpyrrolidone such as crospovidone. For example, about 1%
(wt/wt) to about 10% (wt/wt), about 1% (wt/wt) to about 5% (wt/wt), or about 1%
(wt/wt) to about 3% (wt/wt) crosslinked polyvinylpyrrolidone may be present in an oral dosage form or a unit of an oral dosage form.

[0202] In some embodiments, an oral dosage form may comprise a fumed TM
silica such as Aerosil. For example, about 0.1% (wt/wt) to about 10% (wt/wt), about 0.1% (wt/wt) to about 1% (wt/wt), or about 0.4% (wt/wt) to about 0.6% (wt/wt) fumed silica may be present in an oral dosage form or a unit of an oral dosage form.

[0203] In some embodiments, an oral dosage form may comprise magnesium stearate. For example, about 0.1% (wt/wt) to about 10% (wt/wt), about 0.1% (wt/wt) to about 1% (wt/wt), or about 0.4% (wt/wt) to about 0.6% (wt/wt) magnesium stearate may be present in an oral dosage form or a unit of an oral dosage form.

[0204] An oral dosage form comprising zoledronic acid or another bisphosphonate or osteoclast inhibitor may be included in a pharmaceutical product comprising more than one unit of the oral dosage form.

Date Recue/Date Received 2021-04-07

[0205] A
pharmaceutical product containing or dosage forms for daily use can contain 28, 29, 30, or 31 units of the oral dosage form for a monthly supply.
An approximately 6 week daily supply can contain 40 to 45 units of the oral dosage form. An approximately 3 month daily supply can contain 85 to 95 units of the oral dosage form. An approximately six-month daily supply can contain 170 to 200 units of the oral dosage form. An approximately one year daily supply can contain 350 to 380 units of the oral dosage form.

[0206] A
pharmaceutical product containing oral dosage forms for weekly use can contain 4 or 5 units of the oral dosage form for a monthly supply. An approximately 2 month weekly supply can contain 8 or 9 units of the oral dosage form. An approximately 6 week weekly supply can contain about 6 units of the oral dosage form. An approximately 3 month weekly supply can contain 12, 13 or 14 units of the oral dosage form. An approximately six-month weekly supply can contain 22 to 30 units of the oral dosage form. An approximately one year weekly supply can contain 45 to 60 units of the oral dosage form,

[0207] A
pharmaceutical product may accommodate other dosing regimes.
For example, a pharmaceutical product may comprise 5 to 10 units of the oral dosage form, wherein each unit of the oral dosage form contains about 40 mg to about 150 mg of zoledronic acid, minodronic acid, or ibandronic acid. Some pharmaceutical products may comprise 1 to 10 units of the oral dosage form, wherein the product contains about 200 mg to about 2000 mg of zoledronic acid, minodronic acid, or ibandronic acid. For such a product, each unit of the oral dosage form may be taken daily for 1 to 10 days or 5 to 10 days during a month, such as at the beginning of a month.

[0208] Some oral dosage forms comprising an osteoclast inhibitor¨such as suitable bisphosphonates like zoledronic acid, minodronic acid, or ibandronic acid or salts thereof¨may have enteric coatings or film coatings. In some embodiments, an oral dosage form of an osteoclast inhibitor comprises a tablet having an enteric coating. In some embodiments, an oral dosage form of an osteoclast inhibitor comprises a capsule having an enteric coating. In some embodiments, an oral dosage form of an osteoclast inhibitor comprises a tablet having a film coating. In some embodiments, an oral dosage form of an osteoclast inhibitor comprises a capsule having a film coating.

Date Recue/Date Received 2021-04-07

[0209] Useful doses for an antibody against RANK or RANKL, such as denosumab, may range from about 0.1 mg/kg to about 20 mg/kg, about 0.75 mg/kg to about 7.5 mg/kg, about 0.1 mg/kg to about 5 mg/kg, about 1 mg/kg to about 2 mg/kg, about 10 mg/kg to about 20 mg/kg, about 12 to about 17 mg/kg, about 15 mg/kg to about 20 mg/kg, about 1 mg/kg, about 1 mg/kg to about 10 mg/kg, or any value bounded by or in between these ranges based on the body weight of the mammal. The chosen dose may be administered repeatedly, particularly for chronic conditions, or the amount per dose may be increased or decreased as treatment progresses. The chosen dose may be administered one or more times per week, monthly, every two months, every three months, every six months, or every year.

[0210] In some embodiments, 60 mg of denosumab is administered subcutaneously to patient in need of treatment. In some embodiments, the administration is repeated every six months.

[0211] There are a number of ways that some part of Compound 1 and/or Compound 2 may be removed from a zoledronic acid product. For example, HPLC, preparative TLC, crystallization, sublimation, or zone purification may be employed.
Solvents that may be useful in HPLC, TLC, or crystallization, may include, but are not limited to, water or organic solvents, such as hexanes, diethyl ether, ethyl acetate, methyl acetate, acetone, acetic acid, acetonitrile, tetrahydrofuran, ethanol, methanol, isopropyl alcohol, chloroform, diethyl ether, toluene, dimethylformamide, benzene, etc. Gradients, or two solvent systems may be employed as well. For example, an HPLC separation may begin by elution with water, after some time eluting with water, an organic solvent, such as acetonitrile, methanol, ethanol, ethyl acetate, acetone, acetic acid, methyl acetate, or another solvent could gradually be added to the water, or may replace the water entirely. Similarly, crystallization or recrystallization may employ a single solvent, or a combination of solvents.
For example, zoledronic acid or a salt thereof, such as a disodium salt, might be recrystallized from water, ethanol, methanol, diethyl ether, methyl acetate, acetic acid, etc., or a combination of these solvents or others. In some embodiments, zoledronic acid or a salt thereof, such as a disodium salt, may be dissolved in one solvent, such as water or acetic acid, and crystallized by a second solvent or solvent system, such as hexane, diethyl ether, chloroform, dichloromethane, ethyl acetate, methyl acetate, acetic acid, ethanol, methanol, or a combination thereof. In some Date Recue/Date Received 2021-04-07 embodiments, a disodium salt of zoledronic acid is dissolved in water, and then crystallized by adding hexane. In some embodiments, a disodium salt of zoledronic acid is dissolved in water, and then crystallized by adding diethyl ether. In some embodiments, a disodium salt of zoledronic acid is dissolved in water, and then crystallized by adding chloroform. In some embodiments, a disodium salt of zoledronic acid is dissolved in water, and then crystallized by adding dichloromethane. In some embodiments, a disodium salt of zoledronic acid is dissolved in water, and then crystallized by adding ethyl acetate. In some embodiments, a disodium salt of zoledronic acid is dissolved in water, and then crystallized by adding methyl acetate. In some embodiments, a disodium salt of zoledronic acid is dissolved in water, and then crystallized by adding acetic acid. In some embodiments, a disodium salt of zoledronic acid is dissolved in water, and then crystallized by adding ethanol. In some embodiments, a disodium salt of zoledronic acid is dissolved in water, and then crystallized by adding methanol. For embodiments employing water and a second solvent, the ratio of water to the second solvent (water:second solvent) may be about 1:100 to about 100:1, about 1:10 to about 1:5, about 1:5 to about 1:4, about 1:4 to about 1:3, about 1:3 to about 1:2, about 1:2 to about 1:1, about 1:1 to about 2:1, about 2:1 to about 3:1, about 3:1 to about 4:1, about 4:1 to about 5:1, or about 1:1 to about 10:1.

[0212] In some embodiments, a combination of two methods recited in the paragraph above may be employed, such as HPLC or TLC and crystallization. In some embodiments, a method may be repeated, such as HPLC, preparative TLC, crystallization, sublimation, or zone purification. In some embodiments, a purification method recited in the paragraph above may be performed twice. In some embodiments, a purification method recited in the paragraph above may be performed three or four times.

[0213] In the examples below, zoledronic acid was administered in the disodium salt form as disodium zoledronate tetrahydrate. No bioavailability enhancing agents were used in the test compositions.
Example 1 Effect of Orally Administered Zoledronic Acid in Rat Model of Inflammatory Pain Date Recue/Date Received 2021-04-07 Method:

[0214] The effect of orally administered zoledronic acid on inflammatory pain was examined using the rat complete Freund's adjuvant (CFA) model.
Inflammatory pain was induced by injection of 100% CFA in a 75 pL volume into the left hind paws of Sprague-Dawley rats on day 0, followed by assessments on days 1-3. Animals were orally administered vehicle (control), zoledronic acid 18 mg/m2 (or 3 mg/kg), zoledronic acid 120 mg/m2 (or 20 mg/kg), or zoledronic acid 900 mg/m2 (or 150 mg/kg) daily on days 1-3. Drug was dissolved in distilled water and prepared fresh daily. Animals were fasted prior to dosing. Under current FDA guidelines for extrapolating starting dosages from animals to humans, dosages expressed in mg/m2 are considered equivalent between mammalian species. Thus, for example, 18 mg/m2 in a rat is considered equivalent to 18 mg/m2 in a human being, while mg/kg in a rat may not be equivalent to 3 mg/kg in a human being.

[0215] Values for inflammatory pain (mechanical hyperalgesia) in the vehicle and drug-treated animals were obtained on day 0 prior to CFA
injection, and at baseline and post-treatment on days 1-3. Pain was assessed using a digital Randall-Selitto device (dRS; IITC Life Sciences, Woodland Hills, CA). Animals were placed in a restraint sling that suspended the animal, leaving the hind limbs available for testing. Paw compression threshold was measured by applying increasing pressure to the plantar surface of the hind paw with a dome-shaped tip placed between the 3rd and 4th metatarsus. Pressure was applied gradually over approximately 10 seconds. Measurements were taken from the first observed nocifensive behavior of vocalization, struggle or withdrawal. A cut-off value of 300 g was used to prevent injury to the animal.

[0216] Reversal of inflammatory pain was calculated according to the formula:
% reversal = ( Post-treatment ¨ Post-CFA baseline)/(Pre-CFA baseline ¨ Post-CFA baseline) x 100.

[0217] The experiment was carried out using 9-10 animals per group.
Results:

[0218] Oral administration of zoledronic acid significantly improved inflammatory pain thresholds compared to vehicle. Pain threshold measurements Date Recue/Date Received 2021-04-07 taken at various times are shown in FIG. 1. Paw compression thresholds in the mg/m2 group were higher than for vehicle during the entire measurement period after 30 minutes from the start of treatment. On day three, paw compression thresholds for both the 18 mg/m2 and 900 mg/m2 groups were greater than for vehicle. An improvement in pain threshold of 49% and 83% from baseline was observed for the 18 mg/m2 and the 900 mg/m2 groups respectively.

[0219] Orally administered zoledronic acid produced a 29% reversal of inflammatory pain at the 18 mg/m2, and a 48% reversal at the 900 mg/m2 dose.
This magnitude of effect is comparable to that obtained with clinical doses of commercially available NSAIDs when tested in a similar model of inflammatory pain.
Under current FDA guidelines, the reference body surface area of a human adult is 1.62 m2. Thus, a daily dose of 18 mg/m2 corresponds to a monthly dose of about 500-560 mg/m2 or a human dose of about 800-900 mg.

[0220]
Surprisingly, the two higher doses resulted in thresholds that were lower than vehicle on the first two days of dosing. The 120 mg/m2 group was approximately equal or inferior to vehicle at all time points during the assessment period. While the 900 mg/m2 group showed effectiveness on day 3, this result was accompanied by significant toxicity necessitating euthanization of all the animals in this group two days after cessation of dosing.
Example 2 Effect of Orally Administered Zoledronic Acid in Rat Model of Arthritis Pain Method:

[0221] The effect of orally administered zoledronic acid on arthritis pain was examined in the rat complete Freund's adjuvant (CFA) model of arthritis pain. In this model, injection of 100% complete Freund's adjuvant (CFA) in a 75 pL
volume into the left hind paws is followed by a 10-14 day period to allow for the development of arthritis pain. Animals were orally administered vehicle (control), zoledronic acid 54 mg/m2 (or 9 mg/kg), or zoledronic acid 360 mg/m2 (or 60 mg/kg), divided in three equal daily doses on the first three days post CFA injection. Drug was dissolved in distilled water and prepared fresh daily. Animals were fasted prior to dosing.

[0222] Arthritis pain (mechanical hyperalgesia) in the vehicle and drug-treated animals was evaluated on day 14 post CFA injection using a digital Randall-Date Recue/Date Received 2021-04-07 Selitto device (dRS; IITC Life Sciences, Woodland Hills, CA). Animals were placed in a restraint sling that suspended the animal, leaving the hind limbs available for testing. Paw compression threshold was measured by applying increasing pressure to the plantar surface of the hind paw with a dome-shaped tip placed between the 3rd and 4th metatarsus. Pressure was applied gradually over approximately 10 seconds. Measurements were taken from the first observed nocifensive behavior of vocalization, struggle or withdrawal. A cut-off value of 300 g was used to prevent injury to the animal.

[0223] Reversal of arthritis pain in the ipsilateral (CFA-injected) paw was calculated according to the formula:
% reversal = (ipsilateral drug threshold ¨ ipsilateral vehicle threshold)/(contralateral vehicle threshold ¨ ipsilateral vehicle threshold) x 100.

[0224] The experiment was carried out using 7-10 animals per group.
Results:

[0225] Oral administration of zoledronic acid significantly improved arthritis pain thresholds compared to vehicle. As shown in FIGS. 2A and 2B, orally administered zoledronic acid produced a dose-dependent reversal of arthritis pain. A
reversal of 33% was observed in the 54 mg/m2 group, and reversal of 54% was observed in the 360 mg/m2 group. Under current FDA guidelines, the reference body surface area of a human adult is 1.62 m2. Thus, 54 mg/m2 in a rat is equivalent to an implied human dose of about 87 mg, and 360 mg/m2 in a rat is equivalent to an implied human dose of about 583 mg.
Example 3. Treatment of Complex Regional Pain Syndrome with Orally Administered Zoledronic Acid.

[0226] The effect of orally administered zoledronic acid was examined in the rat tibia fracture model of complex regional pain syndrome (CRPS). CRPS
was induced in the rats by fracturing the right distal tibias of the animals and casting the fractured hindpaws for 4 weeks, as described in Guo TZ et al. (Pain.
2004;108:95-107). This animal model has been shown to replicate the inciting trauma, natural history, signs, symptoms, and pathologic changes observed in human CRPS
patients (Kingery WS et al., Pain. 2003;104:75-84).

Date Recue/Date Received 2021-04-07

[0227] Animals were orally administered either vehicle (control) or zoledronic acid, in a dosage of 18 mg/m2/day (3 mg/kg/day) for 28 days, starting on the day of fracture and casting. Drug was dissolved in distilled water and administered by gavage. Animals were fasted for 4 hours before and 2 hours after dosing. At the end of the 28-day period, casts were removed, and on the following day, the rats were tested for hindpaw pain, edema, and warmth.
Pain assessments

[0228] Pain was assessed by measuring hyperalgesia, and weight bearing.

[0229] To measure hyperalgesia, an up-down von Frey testing paradigm was used. Rats were placed in a clear plastic cylinder (20 cm in diameter) with a wire mesh bottom and allowed to acclimate for 15 minutes. The paw was tested with one of a series of eight von Frey hairs ranging in stiffness from 0.41 g to 15.14 g. The von Frey hair was applied against the hindpaw plantar skin at approximately midsole, taking care to avoid the tori pads. The fiber was pushed until it slightly bowed and then it was jiggled in that position for 6 seconds. Stimuli were presented at an interval of several seconds. Hindpaw withdrawal from the fiber was considered a positive response. The initial fiber presentation was 2.1 g and the fibers were presented according to the up-down method of Dixon to generate six responses in the immediate vicinity of the 50% threshold. Stimuli were presented at an interval of several seconds.

[0230] An incapacitance device (IITC Inc. Life Science, Woodland, CA, USA) was used to measure hindpaw weight bearing, a postural effect of pain.
The rats were manually held in a vertical position over the apparatus with the hindpaws resting on separate metal scale plates and the entire weight of the rat was supported on the hindpaws. The duration of each measurement was 6 seconds and 10 consecutive measurements were taken at 60-second intervals. Eight readings (excluding the highest and lowest ones) were averaged to calculate the bilateral hindpaw weight-bearing values. Weight bearing data were analyzed as the ratio between right (fracture) and left hindpaw weight bearing values ((2R/(R+L)) x100%).
Edema assessment

[0231] A laser sensor technique was used to determine the dorsal-ventral thickness of the hindpaw. Before baseline testing the bilateral hindpaws were Date Recue/Date Received 2021-04-07 tattooed with a 2 to 3 mm spot on the dorsal skin over the midpoint of the third metatarsal. For laser measurements each rat was briefly anesthetized with isoflurane and then held vertically so the hindpaw rested on a table top below the laser.
The paw was gently held flat on the table with a small metal rod applied to the top of the ankle joint. Using optical triangulation, a laser with a distance measuring sensor was used to determine the distance to the table top and to the top of the hindpaw at the tattoo site and the difference was used to calculate the dorsal-ventral paw thickness.
The measurement sensor device used in these experiments (4381 Precicura, Limab, Goteborg, Sweden) has a measurement range of 200 mm with a 0.01 mm resolution.
Hindpaw temperature measurement

[0232] The temperature of the hindpaw was measured using a fine wire thermocouple (Omega, Stanford, CT, USA) applied to the paw skin. Six sites were tested per hindpaw. The six measurements for each hindpaw were averaged for the mean temperature.
Results

[0233] As illustrated in FIG. 3, treatment with orally administered zoledronic acid reversed pain, restored weight bearing, and prevented edema as compared to vehicle treated animals.

[0234] As illustrated in FIG. 4, von Frey pain thresholds for the right (fracture) hindpaw were reduced by 72% versus the contralateral (normal) hindpaw in vehicle treated animals. Zoledronate treatment reversed fracture induced pain by 77% as compared to vehicle treatment.

[0235] As illustrated in FIG. 5, reduction in weight bearing, a postural effect of pain, was significantly higher in the vehicle treated group as compared to the zoledronic acid treated group. Weight bearing on the fracture hindlimb was reduced to 55% of normal in the vehicle treated group. Zoledronate treatment significantly restored hindlimb weight bearing as compared to vehicle treatment (86% of normal).

[0236] As illustrated in FIG. 6, the expected increase in hindpaw thickness was greater in the vehicle treated group as compared to the zoledronic acid treated group, reflecting the development of edema. Zoledronate treatment reduced hindpaw edema by 60% versus vehicle treatment.
Date Recue/Date Received 2021-04-07

[0237] Zoledronic acid reduced hindpaw warmth by 5% versus vehicle treatment.

[0238] The daily dose in the above experiment was 18 mg/m2/day. Under current FDA guidelines, the reference body surface area of a human adult is 1.62 m2. Thus, a daily dose of 18 mg/m2 corresponds to a monthly dose of about 500-mg/m2 or a human dose of about 800-900 mg.
Example 6. Solubility of Disodium Salt of Zoledronic Acid

[0239] The aqueous solubility of zoledronic acid and disodium zoledronate tetrahydrate was determined. One gram of the test compound was measured in to a beaker. Demineralized water (pH 5.5) was then added in small increments to the test compound, and sonification was applied to the mixture. The procedure was continued until complete dissolution was achieved. Full dissolution was determined to have been reached when a clear solution was present with no visible material.
The volume of water required to reach full dissolution was used to calculate a solubility value expressed in grams per 100 nnL. The procedure was performed for each compound.
Results

[0240] As shown in FIG. 7, the aqueous solubility of disodium zoledronate tetrahydrate is approximately 50 times that of zoledronic acid. Disodium zoledronate tetrahydrate has a solubility of 12.5 g/100 mL compared to only 0.25 g/100 mL
for zoledronic acid.
Example 7. Bioayailability of Orally Administered Zoledronic Acid and Disodium Zoledronate

[0241] Tablets were manufactured containing either pure zoledronic acid or the disodium salt of zoledronic acid (disodium zoledronate tetrahydrate).
Both types of tablets contained 50 mg of zoledronic acid equivalent per tablet.
Identical excipients were used in both types of tablets, with amounts adjusted to account for the difference in molecular weights between the acid and the disodium salt.

[0242] Beagle dogs were orally administered tablets containing 150 mg zoledronic acid equivalent either in the form of disodium zoledronate (Group 1) or pure zoledronic acid (Group 2). Each animal was given three 50 mg equivalent Date Recue/Date Received 2021-04-07 tablets (150 mg total), which were administered together. The animal's oral cavity was wetted with water before placing the tablets on the back of the animal's tongue.
Animals were fasted before and after dosing. Animals were 6 to 9 months of age and weighed 6 to 10 kg on the day of dosing. There were three dogs per group.

[0243] Serial blood samples were collected from each animal by venipuncture of the jugular vein at various points after dosing for measurement of plasma concentrations of zoledronic acid. Blood samples were collected into chilled tubes containing K2EDTA as the anticoagulant. Samples were then centrifuged at approximately 3000 rpm at +4 C for 10 minutes for plasma derivation. Plasma concentrations of zoledronic acid were measured using an LC/MS/MS method.
Results

[0244] The average plasma concentrations of zoledronic acid for each group of dogs is summarized in Table 1 and illustrated in FIG. 8. Detectable plasma levels of zoledronic acid were observed for the entire 48 hours that they were measured.
Table 1 Zoledronic Acid plasma concentrations in beagle dogs Plasma Time concentration (hour) (ng/mL) Group 1 Disodium Zoledronate (N=3) Tablets 0 0.00 (150 mg acid equivalent) 0.25 1193.97 0.5 1852.12 0.75 1776.51 1 1626.56 2 640.57 4 136.93 6 53.11 8 26.97 12 13.74 24 6.78 48 5.39 Group 2 (N=3) Zoledronic Acid Tablets 0 0.00 (150 mg acid equivalent) 0.25 390.92 0.5 846.19 0.75 819.15 Date Recue/Date Received 2021-04-07 Table 1 Zoledronic Acid plasma concentrations in beagle dogs Plasma Time concentration (hour) (ng/mL) 1 831.77 2 477.76 4 90.11 6 28.22 8 15.10 12 6.13 24 3.18 48 1.84

[0245] Disodium zoledronate produced significantly higher plasma levels of zoledronic acid than pure zoledronic acid, indicating improved oral absorption with the salt form. Measured using peak plasma concentrations (Cmax), the disodium salt resulted in a 119% actual and 74% weight-adjusted increase in bioavailability as compared to pure zoledronic acid. Measured using area under the plasma concentration curve (AUC0¨), bioavailability was 84% and 46% greater with the disodium salt than with pure zoledronic acid, on an actual and weight-adjusted basis respectively. The average AUCo¨ for the disodium salt was 4073 ng=hr/mL and the average AUC0¨ for the diacid was 2217 ng=hr/mL. The AUC0¨ was found to be dose proportional. Thus, for beagle dogs similar to those tested, about 3 mg to about 4 mg of the disodium salt would be expected to result in an AUC0¨ of about 100 ng=hr/mL, and about 7 mg to about 8 mg of the disodium salt would be expected to result in an AUC0¨ of about 200 ng=hr/mL.
Example 8

[0246] Tablets were prepared by blending zoledronic acid, either in the form of the free acid or the disodium salt, with identical excipients. For dosage forms with a greater amount of active, the amount of the excipients was reduced proportionally to keep the weight of the tablet at about 100 mg. After blending, the ingredients were compressed at varying pressures, followed by a film coating.
The resulting tablets were then tested for hardness using a Dr. Schleuniger Pharmatron 8M Tablet Hardness Tester. The results are shown in Table 2 and FIG. 9.

Date Recue/Date Received 2021-04-07 Table 2 Compression Force Hardness (kPa) Disodium Disodium Diacid Salt Salt (psi) 50 mg 50 mg 71 mg 800 4.0 8.7 4.8 1100 6.1 11.2 6.8 1500 7.7 13.7 7.4 2000 8.7 16.3 10.7 2400 8.7 11.3 3000 11.4 14.1 4400 12.5 14.9 5500 12.8 18.2 6100 13.0 Example 9

[0247] Some embodiments related to joint pain, bone marrow lesions, and osteoarthritis were conceived as a result of analyzing data from a clinical study.
Some of the results of this study were reported by Laslett et. al. in Ann Rheum Dis 2012;71:1322-1328. Some of the description and data reported below was not published prior to filing the present application. Fifty-two (52) patients with clinical knee osteoarthritis and knee bone marrow lesions (BML) were randomized to receive either intravenous zoledronic acid (5 mg) or placebo in a double blind fashion. All patients had to have at least one bone marrow lesion (BML) in the affected knee on magnetic resonance imaging (MRI). All patients had x-ray of the knee for determination of joint space narrowing (JSN), which was graded according to the Osteoarthritis Research Society International (OARS!) atlas. Patients had either no joint space narrowing (OARS! Grade 0), or greater degrees of joint space narrowing (OARS! Grade 1 and Grade 2). Twenty six patients were treated with zoledronic acid (8, 6, and 12 with OARSI Grades 0, 1, and 2, respectively).
Twenty six patients received placebo (8, 8, and 10 with OARSI Grades 0, 1 and 2, respectively).

[0248] Pain intensity was assessed, at baseline and at three months, using a 100 mm visual analog scale (VAS), with zero representing no pain and 100 representing extreme pain. The change in pain intensity from baseline to 3 months was calculated.

Date Recue/Date Received 2021-04-07

[0249] With zoledronic acid treatment, pain was reduced significantly as compared to placebo in patients with no joint space narrowing (OARS! Grade 0), but not in patients with joint space narrowing (OARS! Grades 1-2). As shown in Table 3 and FIG. 10, average VAS scores were reduced by 15 mm as compared to placebo in the OARS! Grade 0 group, but only by 0.28 as compared to placebo in patients with OARSI Grades 1-2.

[0250] In the zoledronic acid group, average VAS scores at 3 months decreased from baseline by approximately 25 mm and 21 mm in patients with OARS! Grades 0 and 1, respectively, but only by 9 mm in the OARS! Grade 2 patients (FIG. 11).
Table 3. Change in VAS Pain Scores at Three Months by OARS! Grade (mm) OARS! Grade 0 OARS! Grades Zoledronic Acid -24.6 -13.2 Placebo -9.6 -12.9 Difference from -15.0 -0.28 Placebo

[0251] With zoledronic acid treatment, pain was reduced significantly as compared to placebo in patients with baseline VAS pain intensity scores of 50 mm or greater, but not in patients with baseline VAS scores less than 50 mm. As shown in Table 4, average VAS scores were reduced by 9 mm as compared to placebo in the patients with baseline VAS 50 mm, but only by 0.6 as compared to placebo in patients with baseline VAS < 50 mm.
Table 4. Change in VAS Pain Scores at Three Months by Baseline VAS (mm) Baseline VAS 2 50 Baseline VAS <50 mm mm Zoledronic Acid -26.2 -7.3 Placebo -17.2 -6.7 Difference from -9.0 -0.6 Placebo Date Recue/Date Received 2021-04-07

[0252] As summarized in Table 5 and illustrated in FIG. 12, pain reduction was greater in patients with baseline VAS 50 mm, greater still in patients with OARSI Grade 0 joint space narrowing, and greatest in patients with both baseline VAS 50 mm and OARSI Grade 0 joint space narrowing.
Table 5. Pain Reduction Compared to Placebo at Three Months (mm) VAS Change All patients -4.8 Baseline VAS 50 mm -9.0 OARS! Grade 0 -15.0 Baseline VAS 50 mm + OARS! -19.4 Grade 0

[0253] BMLs were evaluated using proton density-weighted fat saturation MR images. BMLs were scored using Osiris software (University of Geneva, Geneva, Switzerland). The maximum size was measured in mm2 using software cursors applied to the greatest area of each lesion. The lesion with the highest score was used if more than one was present at the same site. Each patient was given a BML score (mm2) at each of the four sites (medial tibial, medial femoral, lateral tibial, and lateral femoral sites) and these were summed to create a total BML score (mm2). The change in the total area of BMLs from baseline to 6 months was calculated.

[0254] The size of BMLs was reduced with zoledronic acid treatment. As shown in FIG. 13 and Table 6, average BML area decreased by approximately 190 mm2 as compared to placebo in the OARS! Grade 0 group, but only by approximately 33 mm2 as compared to placebo in patients with OARSI Grades 1-2.
Table 6. Change in BML Size (mm2) OARS! Grade 0 OARS! Grades Zoledronic Acid -244 -117 Placebo -55 -84 Difference from Placebo Date Recue/Date Received 2021-04-07 Example 10 Methods

[0255] A study was performed to evaluate the efficacy of a single intravenous infusion of 5 mg ZA in comparison with intravenous placebo infusion among patients with chronic low back pain (LBP) and Modic changes on MRI. This study was a double-blinded, randomized, placebo-controlled clinical trial in patients with low back pain (LBP). Patients were included in the study if they had low back symptoms for at least three months, a LBP of at least six (6) on a 10-cm Visual Analog Scale (VAS) or an Oswestry Disability Index (OD!) of at least 30%, and an Ml, mixed M1/2 or M2 type change on MRI performed within six months at most prior to enrolment.

[0256] Patients were excluded from the study if they had renal impairment with reduced creatinine clearance defined as an estimated glomerular filtration rate (eGFR) below 40 ml/min, hypocalcemia, known hypersensitivity to zoledronic acid or other bisphosphonates or ingredients of the infusion product, the presence of red flags, nerve root entrapment or willingness for early retirement.
Premenopausal women of childbearing potential were also excluded. Blood samples were taken prior to the infusion to assess the serum concentration of calcium and creatinine.
The clinical examination included medical history and clinical assessment of lumbar flexibility, tendon signs, and motor and sensory testing.

[0257] After confirmation of eligibility patients were randomized to receive a single intravenous infusion of 5 mg zoledronic acid (n = 20) or 100 ml saline as placebo (n = 20) over a 15-minute period. Information on use of the concomitant medication and hospital admissions were recorded. Blood samples were taken for the assessment of safety, inflammatory mediators and markers of bone turnover at baseline, one month and one year.

[0258] Clinical assessments were performed 14 days before enrolment (screening visit), and follow-up visits at one month and one year after the infusion.
The primary outcome was the change in the intensity of LBP on VAS. Secondary outcomes included leg pain intensity, ODI, health-related quality of life assessed with RAND-36, patient-reported sick leaves and lumbar flexibility. These outcome measures were assessed at baseline and at each follow-up. Lumbar flexibility was Date Recue/Date Received 2021-04-07 evaluated using the fingers-to-floor and trunk side bending measures (in cm).
The percentage of patients undergoing a 20% relative improvement, the proportion of patients reaching a VAS score of 40 or less in the primary outcome, and patient acceptable symptom state (PASS) were also assessed. Pain medication use was inquired about during the follow-up visits.
Results

[0259] Zoledronic acid treatment resulted in a greater improvement in LBP
intensity at one month as compared to placebo treatment. Furthermore, the patients receiving zoledronic acid reported NSAID use at one year significantly less often than those in the placebo group. Overall, the improvements in most of the evaluated parameters were greater in the zoledronic acid group throughout the follow-up period.

[0260] The clinical characteristics of study participants at baseline are displayed in Table 6. The mean LBP duration was 293 days, initial LBP
intensity on VAS 6.7, leg pain on VAS 2.9 and the ODI score was 32%. Altogether 19 patients in the ZA group and 18 in the placebo group had a M1/2 lesion. Modic changes were most commonly (70%) situated at L4/5 or L5/S1. The zoledronic acid and placebo groups were similar as regards the demographic and background characteristics of all patients at baseline (Table 6).

[0261] The mean difference (MD) between the treatment groups in the primary outcome, intensity of LBP, significantly favored zoledronic acid at one month (MD 1.4; 95% Cl 0.01 to 2.9) while at one year no significant difference was observed (MD 0.7; 95% Cl -1.0 to 2.4; Table 7). The proportion of patients with at least 20% improvement in intensity of LBP and PASS both favored the zoledronic acid treatment at one month: zoledronic acid 55% vs. placebo 25% (p = 0.105) and zoledronic acid 50% vs. placebo 20% (p = 0.096), respectively.

[0262] For the patients who were treated with zoledronic acid, the reduction in pain intensity was greater in those with greater baseline pain intensity as shown in Table 9. The mean reduction in pain from baseline was 3.4 for patients with baseline pain intensity 7, as compared to a reduction of only 0.1 for patients with a baseline pain intensity < 6.

Date Recue/Date Received 2021-04-07

[0263] Of the secondary outcomes, the improvement in ODI, favored zoledronic acid at 1 month, the adjusted between-group difference being 6.0%
(95%
Cl -0.6 to 13), but not at one year (Table 7). Similarly, side bending (to right and left) favored the zoledronic acid treatment at one month but not at one year (Table 7).
Changes in total RAND-36, and in the physical and mental components of RAND-36 are shown in Table 8.

[0264] At baseline, there were no differences in self-reported use of non-steroidal anti-inflammatory drugs (NSAIDs) between the treatment groups, whereas at one year, only 20% of patients in the ZA group used NSAIDs versus 60% in the placebo group.
Table 6: Baseline characteristics of study population according to treatment group Zoledronic Pacebo Characteristics Acid n=20 n=20 Sex, n ( /0) men 15 (75) 11(55) Age, mean (SD) years 49 (9.3) 51(7.3) Smoking, n (%) regular smokers* 5 (25) 6 (30) BMI, mean (SD) kg/m 26 ,3.3) 27 (3.2) Workload, n (%) -Sedentary work with limited walking 4 (20) 4 (22) -Fairly light work with considerable walking 4 (20) 3 (17) but no lifting or carrying heavy objects -Fairly strenuous work with walking and 8 (40) 6 (33) lifting heaving objects or climbing stairs or uphill -Very strenuous work with lifting or carrying 4 (20) 5 (28) heaving objects such as shoveling, digging, or hammering Type of worst MC-lesion**, n - Type I 1 1 -Type I/II 19 18 - Type II 0 1 MC at two or more levels, n (%) 7 (3.5) 4 (20) Levels of MC, n Duration of LBP, median (IQ range) days 330 (200, 365) 315 (270, 365) Intensity of LBP, mean (SD)*** 6.6 (1.4) 6.8 (1.6) Duration of leg pain, median (IQ range) days 50(0, 100) 36(0, 160) Date Recue/Date Received 2021-04-07 Zoledronic Pacebo Characteristics Acid n=20 n=20 Intensity of leg pain, mean (SD)*** 3.0 (3.1) 2.9 (2.3) Oswestry Disability Index, %, Mean (SD) 30 (11) 35 (10) Duration of sick leave during the past year, 14 (0, 48) 18(1, 181) median (IQ range) days RAND-36, mean (SD) 50 (8) 50 (7) RAND-36 physical component, mean (SD) 51(8) 49 (8) RAND-36 mental component, mean (SD) 51(8) 49 (9) BMI = Body Mass Index, MC = Modic Change, LBP = low back pain, SD = standard deviation, IQ = inter-quartile.
*Smoking at least one cigarette per day.
**If different types of MC at two or more levels, classification is based on the assumed severity of the type, i.e., Type I > mixed Type I/II > Type II.
***Assessed using a 10 cm Visual Analogue Scale (VAS).
Table 7. Low back symptoms and lumbar flexibility at baseline, one month and months according to treatment group and between group comparisons of difference from baseline to one month and 12 months Mean (SD) Mean (SD) Unadjusted Adjusted original values change analyses analyses ZA Placeb ZA Placeb Differenc P Differen P*
n=20 o o e ce n=20 (95% Cl) (95% Cl) Intensity of LBP
- 6.6 6.8 Baseline (1.4) (1.6) 1.3 1.4 0.09 0.04 - 1 mo. 4'3 (2.3) (2.2) (2.7) (2 (-0.2 to (0.01 to .1) 7 9 - 12 3.8 4.6 -2.8 -2.2 0. 0.47 0. 0.38 mos. (2.5) (2.9) (2.9) (2.5) (-1.1 to (-1.0 to Intensity of leg paina - 3.0 2.9 Baseline (3.1) (2.3) 3.0 -0.6 0.1 0.36 0.23 (-0..8 0.8 - 1 mo. 2.0 09 to (-0.6 to (2.3) (2.4) (2.4) (2.6) 7 7 - 12 2.1 2.7 -0.9 -0.3 0.6 0.57 to (-1.3 to 0.57 0. 0.57 mos. (2.8) (2.6) (3.4) (3.0) 3 3 Oswestry disability index, %
-Baseline. 30(11) 35(10) 4.3 -5.9 -1.7 0.21 6.0 0.07 - 1 mo. 24 (10) 33 (13) 7 (-2.5 to 2 (-0.6 to 1 11) 13) m Date Recue/Date Received 2021-04-07 Mean (SD) Mean (SD) Unadjusted Adjusted original values change analyses analyses ZA Placeb ZA Placeb Differenc P Differen P*
n=20 o o e ce n=20 (95% Cl) (95% Cl) 3.1 5.1 - 12 -5.0 -1.9 0.47 0.23 mos. (15) (12) 25 (13) 33 (15) (-5.6 to (-3.4 to 12) 14) Fingers-to-floor, cm Baseli.ne 23(19) 19(18) -5.1 - 5.0 3.6 0.1 0 - 1 mo. 17 (17) 19(17) (-4.8 to (-5.0 to .40 0.306 3 15) 12) 7.1 5.3 - 12 -6.3 0.9 0.21 0.27 mos. (23) (11) 16 (16) 20 (19) (-4.3 to (-4.5 to 18) 15) Sidebending to right, cm 14.1 13.8 Baseline (4.9) (7.2) 15.7 13.3 1.5 -0.5 -2.0 0.10 -2.0 0.08 .3 to (-4.4 to - 1 mo. (-4 (5.9) (6.9) (4.7) (2.2) 1 7 - 12 15.7 13.8 1.6 -0.1 -1' (-4.3 to (-4.2 to 0.22 -1'7 0.18 mos. (5.6) (6.5) (4.8) (3.5) 7 0 1.1) 0.8) Sidebending to left, cm - 15.0 13.3 Baseline (5.4) (5.5) 16.1 12.8 1.1 -0.5 -1'5 0.07 -1'7 0.05 . - 1 mo. (-32 to (-3.4 to (5.3) (5.9) (3.0) (2.2) 2 1 - 12 16.2 13.7 1.2 0.5 -0.7 -1.0 (-3.5 to (-3.8 to 0.60 0.45 mos. (6.7) (5.7) (5.3) (3.2) 1 8 SD = standard deviation, Cl = confidence interval, ZA = zoledronic acid, LBP =
low back pain.
*ANCOVA: Difference between follow-up and baseline, treatment effect adjusted for baseline value.
aOne subject missing at baseline in placebo group and in ZA group, and one subject at 1 month in ZA group.
Table 8: Health-related quality of life assessed using RAND-36 at baseline, one month, and 12 months according to treatment group and between group comparisons of difference from baseline to one month and 12 months Date Recue/Date Received 2021-04-07 Mean (SD) Mean (SD) Unadjusted Adjusted original values change analyses analyses ZA Placeb ZA Placeb Differenc P Differenc P*
n=20 n=20 (95% Cl) (95% Cl) Total RAND-36 50 (8) 50 (7) Baseline - 1 mo. 51(8) 49 (8) 0.6 -0.6 1.2 (-3 to 0.53 1.3 (-3 to 0.47 (6.4) (5.0) 5) 0 5) 7 - 12 51(8) 49(9) 1.0 -1.0 2.1 (-3t0 0.37 2.2 (-2 to 0.31 mos. (8.7) (5.9) 7) 8 7) 4 Physical component 52 (8) 48 (8) Baseline -1 mo. 52(9) 48(8) 0.1 -0.1 0.3 (-4 to 0.89 1.3 (-3 to 0.55 (8.6) (5.5) 5) 7 6) 4 - 12 52 (8) 48 (2) 0.3 -0.3 0.7 (-5 to 0.80 2.1 (-3 to 0.40 mos. (10) (6.5) 6) 8 7) 5 Mental component 49 (9) 51(8) Baseline - 1 mo. 50 (9) 50 (9) 1,0 -1.0 2.0 (-2 to 0.28 1.6 (-2 to 0.39 (6.1) (5.6) 6) 6 5) 6 - 12 51(9) 49(9) 1,8 -1.8 3.5 (-2 to 0.16 2.7 (-2 to 0.26 mos. (9.0) (6.7) 9) 7 7) 1 SD = standard deviation, Cl = confidence interval, ZA = zoledronic acid.
*ANCOVA: Difference between follow-up and baseline, treatment effect adjusted for baseline value.
Table 9. Pain Reduction in Patients Treated Zoledronic Acid (cm) VAS Change from Baseline Baseline VAS <6 -0.1 Baseline VAS 6 and <7 -2.3 Baseline VAS 7 -3.4 Example 11 Date Recue/Date Received 2021-04-07 a / HCI, water 0 N' /
99.7% 0 87.7% HO

-+ -+

PO3H2 /_\

or HO
Compound 1 Compound 2

[0265] 1,3-Bis(2-methoxy-2-oxoethyl)-1H-imidazol-3-ium chloride (9).
Methyl chloroacetate (2; 29.8 mL, 338.6 mmol, 2.0 eq) was added drop-wise to 1-(trimethylsily1)-1H-imidazole (8; 25.0 mL, 169.3 mmol). The mixture was heated at 60 C for 24 hours. The mixture was cooled to room temperature, washed with Et20 (3x500 mL) and dried in vacuo yielding 9 (41.97 g, 168.8 mmol, 99.7%) as a white solid.

[0266] 1,3-Bis(carboxymethy1)1H-imidazol-3-ium chloride (10). To 1,3-bis(2-methoxy-2-oxoethyl)-1H-imidazol-3-ium chloride (9; 41.00 g, 164.88 mmol, eq.) was added 37% aq. HCI (30.03 mL, 362.74 mmol, 2.2 eq.). The mixture was stirred under reflux for 0.5 hour. The mixture was concentrated and the remaining solid was washed with acetone (2x200 mL) and Et20 (3x200 mL). Drying in in vacuo gave 10 (31.89 g, 144.55 mmol, 87.7%) as a white solid.

[0267] Compound 1: Compound 10 is reacted with an equimolar amount of phosphorous acid, followed by an equimolar amount of phosphorous trichloride, and an excess of water to form Compound 1, which is precipitated from ethanol.

[0268] Compound 2: 1,3-Bis(carboxymethyl)-1H-imidazol-3-ium chloride (10, 2.00 g, 9 mmol, 1.0 eq) and H3P03 (7.37 g, 90 mmol, 10 eq) were dissolved in toluene (10 mL) and heated to 70 C. The reaction mixture was stirred at this temperature for 20 min before PCI3 (16 mL, 180 mmol, 20 eq) was added within min. The reaction mixture was then heated to 95 C and stirred at this temperature for 2 h. Then, aq. HCI (30 mL, 37% HCI and 5 mL H20) was added. The reaction Date Recue/Date Received 2021-04-07 mixture was heated to 100 C and stirred at this temperature for 7 h, for 2 d stirred at room temperature and then filtered. The filtrate was cooled in an ice bath and added within 45 min to absolute Et0H (90 mL). The resulting turbid solution was stirred for 1 h at room temperature before the solid was filtered off. The filter cake (46-1) was isolated and analyzed by 2D-NMR spectroscopy and mass spectrometry (m/z= 477).

The filtrate was concentrated in vacuo to give residue 46-2. Five hundred mg of this residue were treated with aq. NaOH (150 mg in 3.5 mL H20) and to this was added Et0H (7 mL). After standing overnight the liquid was decanted and the resulting solid (46-M4) was analyzed NMR and mass spectrometry (m/z= 477).

[0269] The following embodiments are specifically contemplated:
Embodiment 1. A method of relieving inflammatory pain comprising administering an oral dosage form containing zoledronic acid to a mammal in need thereof, wherein the mammal receives a total monthly dose of zoledronic acid that is about 800 mg/m2 or less based upon the body surface area of the mammal.
Embodiment 2. The method of embodiment 1, wherein the mammal is a human being that receives a total monthly dose of zoledronic acid that is about 30 mg/m2 to about 700 mg/m2.
Embodiment 3. The method of embodiment 2, wherein the total monthly dose is administered in 4 or 5 weekly doses.
Embodiment 4. The method of embodiment 2, wherein the total monthly dose is administered in 28 to 31 daily doses.
Embodiment 5. The method of embodiment 2, wherein the total monthly dose is administered in 5 to 10 individual doses during the month.
Embodiment 6. The method of embodiment 1, wherein the mammal is a human being that receives a total weekly dose of zoledronic acid that is about 10 mg to about 300 mg.
Embodiment 7. The method of embodiment 6, wherein the total weekly dose is a single dose, administered once a week.
Embodiment 8. The method of embodiment 6, wherein the total weekly dose is administered in 2 to 7 individual doses during the week.

Date Recue/Date Received 2021-04-07 Embodiment 9. The method of embodiment 1, wherein the mammal is a human being that receives a total weekly dose of zoledronic acid that is about 10 mg to about 150 mg.
Embodiment 10. The method of any preceding embodiment, wherein the mammal experiences significant pain relief more than 3 hours after administration of the dosage form.
Embodiment 11. The method of embodiment 10, wherein the mammal experiences significant pain relief during at least a part of a time from about 3 hours to about 24 hours after administration of the dosage form.
Embodiment 12. The method of embodiment 10, wherein the mammal experiences significant pain relief during at least a part of a time from about 3 hours to about 3 weeks after administration of the dosage form.
Embodiment 13. A method of relieving inflammatory pain comprising administering an oral dosage form containing zoledronic acid to a mammal in need thereof, wherein the oral dosage form contains about 10 mg/m2 to about 20 mg/m2 of zoledronic acid based upon the body surface area of the mammal.
Embodiment 14. The method of embodiment 13, wherein the oral dosage form contains about 15 mg/m2 to about 20 mg/m2 of zoledronic acid based upon the body surface area of the mammal.
Embodiment 15. A method of relieving inflammatory pain comprising orally administering to a mammal in need thereof, about 300 mg/m2 to about 600 mg/m2 of zoledronic acid per month to the mammal, based upon the body surface area of the mammal.
Embodiment 16. The method of embodiment 15, comprising orally administering about 450 mg/m2 to about 600 mg/m2 of zoledronic acid per month to the mammal, based upon the body surface area of the mammal.
Embodiment 17. The method of any preceding embodiment, wherein the mammal is not suffering from bone metastasis.
Embodiment 18. The method of any preceding embodiment, wherein the mammal is not suffering from cancer.
Embodiment 19. The method of any preceding embodiment, wherein the zoledronic acid is administered as a salt of a dianion of zoledronic acid.
Date Recue/Date Received 2021-04-07 Embodiment 20. A method of relieving pain associated with an arthritis comprising administering an oral dosage form containing zoledronic acid to a human being in need thereof.
Embodiment 21. The method of embodiment 20, wherein the human being receives a total monthly dose of zoledronic acid that is about 40 mg to about mg.
Embodiment 22. The method of embodiment 21, wherein the total monthly dose is administered in 4 or 5 weekly doses.
Embodiment 23. The method of embodiment 21, wherein the total monthly dose is administered in 28 to 31 daily doses.
Embodiment 24. The method of embodiment 21, wherein the total monthly dose is administered in 5 to 10 individual doses during the month.
Embodiment 25. The method of embodiment 20, wherein the human being receives a total weekly dose of zoledronic acid that is about 100 mg to about mg.
Embodiment 26. The method of embodiment 25, wherein the total weekly dose is a single dose, administered once a week.
Embodiment 27. The method of embodiment 25, wherein the total weekly dose is administered in 2 to 7 individual doses during the week.
Embodiment 28. The method of embodiment 20, wherein the human being receives a total weekly dose of zoledronic acid that is about 10 mg to about 100 mg.
Embodiment 29. The method of any of embodiments 20-28, wherein the human being experiences significant pain relief more than 3 hours after administration of the dosage form.
Embodiment 30. The method of embodiment 29, wherein the human being experiences significant pain relief during at least a part of a time from about 3 hours to about 24 hours after administration of the dosage form.
Embodiment 31. The method of embodiment 29, wherein the human being experiences significant pain relief during at least a part of a time from about 3 hours to about 3 weeks after administration of the dosage form.
Embodiment 32. The method of any of embodiments 20-31, wherein the dosage form contains about 10 mg/m2 to about 20 mg/m2 of zoledronic acid based upon the body surface area of the human being.

Date Recue/Date Received 2021-04-07 Embodiment 33. The method of embodiment 32, wherein the dosage form contains about 15 mg/m2 to about 20 mg/m2 of zoledronic acid based upon the body surface area of the human being.
Embodiment 34. The method of any of embodiments 20-33, wherein about 50 mg/m2 to about 200 mg/m2 of zoledronic acid is orally administered per month, based upon the body surface area of the human being.
Embodiment 35. The method of any of embodiments 20-31, wherein the dosage form contains about 80 mg/m2 to about 150 mg/m2 of zoledronic acid based upon the body surface area of the human being.
Embodiment 36. The method of embodiment 35, wherein about 300 mg/m2 to about 1000 mg/m2 of zoledronic acid is orally administered per month, based upon the body surface area of the human being.
Embodiment 37. The method of any of embodiments 20-36, wherein the human being is not suffering from bone metastasis.
Embodiment 38. The method of any of embodiments 20-37, wherein the human being is not suffering from cancer.
Embodiment 39. The method of any preceding embodiment, wherein the zoledronic acid is in the disodiunn salt form.
Embodiment 40,. An oral dosage form comprising zeiedronic acid, wherein the oral bioavailability of zoledronic acid in the dosage form is about 0,01%
to about 4%.
Embodiment 41, The oral dosage form of embodiment 40, wherein the oral dosage form contains about 10 mg to about 300 mg of zoledronic acid.
Embodiment 42, The oral dosage form of embodiment 40, wherein the oral dosage form contains about 10 mg to about 50 mg of zole,cironic acid.
Embodiment 43. The oral dosage form of any of embodiments 40-42, wherein the oral bioavailability of zoledronic acid in the dosage form is about 0.1% to about 2%.
Embodiment 44. A pharmaceutical product comprising more than one unit of an oral dosage form of embodiment 40.
Embodiment 45. The pharmaceutical product of embodiment 44, wherein each unit of the oral dosage form contains about 1 mg to about 50 mg of zoledronic acid.

Date Recue/Date Received 2021-04-07 Embodiment 46. The pharmaceutical product of embodiment 45, comprising 28. 29, 30, or 31 units of the oral dosage form, for a total of about 28 mg to about 1600 mg of zoledronic acid to be administered in about 1 month.
Embodiment 47. The pharmaceutical product of embodiment 45, comprising 85 to 95 units of the oral dosage form, for a total of about 85 mg to about 4800 mg of zoledronic acid to be administered in about 3 months.
Embodiment 48. The pharmaceutical product of embodiment 45, comprising 170 to 200 units of the oral dosage form, for a total of about 170 mg to about 10,000 mg of zoledronic acid to be administered in about 6 months.
Embodiment 49. The pharmaceutical product of embodiment 45, comprising 350 to 380 units of the oral dosage form, for a total of about 350 mg to about 19,000 mg of zoledronic acid to be administered in about 1 year.
Embodiment 50. The pharmaceutical product of embodiment 44, wherein each unit of the oral dosage form contains about 10 mg to about 300 mg.
Embodiment 51. The pharmaceutical product of embodiment 50, comprising 4 or 5 units of the oral dosage form, for a total of about 40 mg to about 1500 mg of zoledronic acid to be administered within a period of about 1 month.
Embodiment 52. The pharmaceutical product of embodiment 50, comprising 8 or 9 units of the oral dosage form, for a total of about 80 mg to about 2700 mg of zoledronic acid to be administered in about 2 months.
Embodiment 53. The pharmaceutical product of embodiment 50, comprising 12. 13 or 14 units of the oral dosage form, for a total of about 120 mg to about 4200 mg of zoledronic acid to be administered in about 3 months.
Embodiment 54. The pharmaceutical product of embodiment 50, comprising 22 to 30 units of the oral dosage form, for a total of about 220 mg to about 9000 mg of zoledronic acid to be administered in about 6 months.
Embodiment 55. The pharmaceutical product of embodiment 50, comprising 45 to 60 units of the oral dosage form, for a total of about 450 mg to about 18000 mg of zoledronic acid to be administered in about 1 year.
Embodiment 56. The pharmaceutical product of embodiment 44, comprising 1 to 10 units of the oral dosage form, wherein the product contains about 200 mg to about 2000 mg of zoledronic acid.
Embodiment 57. The oral dosage form of any preceding embodiment, wherein the zoledronic acid is in the form of a sodium salt.

Date Recue/Date Received 2021-04-07 Embodiment 58. The oral dosage form of any preceding embodiment, wherein the zoledronic acid is in a form that has an aqueous solubility greater than 15'0 (w/v).
Embodiment 59. The oral dosage form of any preceding embodiment, wherein the zoledronic acid is in a form that has an aqueous solubility of about 5%
(Nv/v ) to about 50% (w/v ).
Embodiment 60. An oral dosage form comprising zoiedronic acid and an excipient, wherein the zoledronic acid is in a form that has an aqueous solubility greater than 1% (w/v).
Embodiment 61. The oral dosage form of embodiment 60, wherein the zoledronic acid is in a form that has an aqueus solubility of about 5% (w/v) to about 50% (w/v).
Embodiment 62. A method of treating complex regional pain syndrome comprising administering an oral dosage form containing zoledronic acid to a mammal in need thereof.
Embodiment 63. The method of embodiment 62, wherein the mammal is a human being that receives an amount of zoledronic acid that is about 30 mg/m2 to about 700 mg/m2 in a period of one month or less.
Embodiment 64. The method of embodiment 63, wherein 4 or 5 weekly doses are administered in a period of one month or less.
Embodiment 65. The method of embodiment 63, wherein 28 to 31 daily doses are administered in a period of one month or less.
Embodiment 66. The method of embodiment 63, wherein 5 to 10 individual doses are administered during a period of one month or less.
Embodiment 67. The method of embodiment 63, wherein about 30 mg/m2 to about 700 mg/m2 of zoledronic acid is administered during only one month.
Embodiment 68. The method of embodiment 63, wherein about 30 mg/m2 to about 700 mg/m2 of zoledronic acid is administered in a period of one month or less for 2 or more consecutive months.
Embodiment 69. The method of embodiment 62, wherein the mammal receives about 10 mg/m2 to about 30 mg/m2 of zoledronic acid daily.
Embodiment 70. The method of embodiment 62, wherein the mammal is a human being that receives a total weekly dose of zoledronic acid that is about 10 mg to about 300 mg.

Date Recue/Date Received 2021-04-07 Embodiment 71. The method of embodiment 70, wherein the total weekly dose is a single dose, administered once a week.
Embodiment 72. The method of embodiment 70, wherein the total weekly dose is administered in 2 to 7 individual doses during the week.
Embodiment 73. The method of any of embodiments 62-72, wherein the complex regional pain syndrome is complex regional pain syndrome type I.
Embodiment 74. The method of any of embodiments 62-72, wherein the complex regional pain syndrome is complex regional pain syndrome type II.
Embodiment 75. The method of any preceding embodiment, wherein the zoledronic acid is in a salt form.
Embodiment 76. The method of any of embodiments 62-75, wherein the dosage form contains about 10 mg/m2 to about 20 mg/m2 of zoledronic acid based upon the body surface area of the mammal.
Embodiment 77. The method of embodiment 76, wherein the dosage form contains about 15 mg/m2 to about 20 mg/m2 of zoledronic acid based upon the body surface area of the mammal.
Embodiment 78. A method of treating complex regional pain syndrome, comprising administering pamidronic acid to a human being in need thereof.
Embodiment 79. A method of treating complex regional pain syndrome, comprising administering neridronic acid to a human being in need thereof.
Embodiment 80. A method of treating complex regional pain syndrome, comprising administering olpadronic acid to a human being in need thereof.
Embodiment 81. A method of treating complex regional pain syndrome, comprising administering alendronic acid to a human being in need thereof.
Embodiment 82. A method of treating complex regional pain syndrome, comprising administering incadronic acid to a human being in need thereof.
Embodiment 83. A method of treating complex regional pain syndrome, comprising administering ibandronic acid to a human being in need thereof.
Embodiment 84. A method of treating complex regional pain syndrome, comprising administering risedronic acid to a human being in need thereof.
Date Recue/Date Received 2021-04-07 Embodiment 85. A method of treating pain, comprising administering pamidronic acid to a human being in need thereof.
Embodiment 86. A method of treating pain, comprising administering neridronic acid to a human being in need thereof.
Embodiment 87. A method of treating pain, comprising administering olpadronic acid to a human being in need thereof.
Embodiment 88. A method of treating pain, comprising administering alendronic acid to a human being in need thereof.
Embodiment 89. A method of treating pain, comprising administering incadronic acid to a human being in need thereof.
Embodiment 90. A method of treating pain, comprising administering ibandronic acid to a human being in need thereof.
Embodiment 91. A method of treating pain, comprising administering risedronic acid to a human being in need thereof.
Embodiment 92. A method of treating arthritis pain, comprising administering pamidronic acid to a human being in need thereof.
Embodiment 93. A method of treating arthritis pain, comprising administering neridronic acid to a human being in need thereof.
Embodiment 94. A method of treating arthritis pain, comprising administering olpadronic acid to a human being in need thereof.
Embodiment 95. A method of treating arthritis pain, comprising administering alendronic acid to a human being in need thereof.
Embodiment 96. A method of treating arthritis pain, comprising administering incadronic acid to a human being in need thereof.
Embodiment 97. A method of treating arthritis pain, comprising administering ibandronic acid to a human being in need thereof.
Embodiment 98. A method of treating arthritis pain, comprising administering risedronic acid to a human being in need thereof.

Date Recue/Date Received 2021-04-07 Embodiment 99. A method of treating inflammatory pain, comprising administering pamidronic acid to a human being in need thereof.
Embodiment 100. A method of treating inflammatory pain, comprising administering neridronic acid to a human being in need thereof.
Embodiment 101. A method of treating inflammatory pain, comprising administering olpadronic acid to a human being in need thereof.
Embodiment 102. A method of treating inflammatory pain, comprising administering alendronic acid to a human being in need thereof.
Embodiment 103. A method of treating inflammatory pain, comprising administering incadronic acid to a human being in need thereof.
Embodiment 104. A method of treating inflammatory pain, comprising administering ibandronic acid to a human being in need thereof.
Embodiment 105. A method of treating inflammatory pain, comprising administering risedronic acid to a human being in need thereof.
Embodiment 106. A method of treating complex regional pain syndrome, comprising administering etidronic acid to a human being in need thereof.
Embodiment 107. A method of treating pain, comprising administering etidronic acid to a human being in need thereof.
Embodiment 108. A method of treating arthritis pain, comprising administering etidronic acid to a human being in need thereof.
Embodiment 109. A method of treating inflammatory pain, comprising administering etidronic acid to a human being in need thereof.
Embodiment 110. A method of treating complex regional pain syndrome, comprising administering clodronic acid to a human being in need thereof.
Embodiment 111. A method of treating pain, comprising administering clodronic acid to a human being in need thereof.
Embodiment 112. A method of treating arthritis pain, comprising administering clodronic acid to a human being in need thereof.

Date Recue/Date Received 2021-04-07 Embodiment 113. A method of treating inflammatory pain, comprising administering clodronic acid to a human being in need thereof.
Embodiment 114. A method of treating complex regional pain syndrome, comprising administering tiludronic acid to a human being in need thereof.
Embodiment 115. A method of treating pain, comprising administering tiludronic acid to a human being in need thereof.
Embodiment 116. A method of treating arthritis pain, comprising administering tiludronic acid to a human being in need thereof.
Embodiment 117. A method of treating inflammatory pain, comprising administering tiludronic acid to a human being in need thereof.
Embodiment 118. The method of any of embodiments 78-117, wherein the active compound is orally administered.
Embodiment 119. The method of any of embodiments 78-117, wherein the active compound is parenterally administered.
Embodiment 120. A method of enhancing the oral bioavailability of zoledronic acid comprising orally administering a dosage form containing zoledronic acid in the disodium salt form.
Embodiment 121. The method of embodiment 120, wherein the zoledronic acid in the disodium salt form provides an enhancement to bioavailability, as compared to zoledronic acid in the diacid form, which adds to any enhancement to bioavailability provided by any bioavailability-enhancing agents in the dosage form.
Embodiment 122. The method of embodiment 120, wherein the dosage form is substantially free of bioavailability-enhancing agents.
Embodiment 123. The method of embodiment 120, wherein the zoledronic acid in the disodium salt form is administered to a mammal in an amount that provides an area under the plasma concentration curve of zoledronic acid of about 4 ng=h/mL to about 2000 ng=h/mL to the mammal each time the zoledronic acid in the disodium salt is administered.
Embodiment 124. The method of embodiment 123, wherein the zoledronic acid in the disodium salt form is administered at an interval of about 3 to about 4 Date Recue/Date Received 2021-04-07 weeks in an amount that provides an area under the plasma concentration curve of zoledronic acid of about 100 ng=h/mL to about 2000 ng=h/mL to the mammal each time the zoledronic acid in the disodium salt form is administered.
Embodiment 125. The method of embodiment 123, wherein the zoledronic acid in the disodium salt form is administered weekly, or 3 to 5 times in a month, in an amount that provides an area under the plasma concentration curve of zoledronic acid of about 20 ng=h/mL to about 700 ng=h/mL to the mammal each time the zoledronic acid in the disodium salt form is administered.
Embodiment 126. The method of embodiment 123, wherein the zoledronic acid in the disodium salt form is administered daily in an amount that provides an area under the plasma concentration curve of zoledronic acid of about 4 ng=h/mL to about 100 ng=h/mL to the mammal each time the zoledronic acid in the disodium salt form is administered.
Embodiment 127. The method of embodiment 120, wherein the dosage form is a solid.
Embodiment 128. The method of embodiment 120, 121, 122, 123, 124, 125, 126, or 127, wherein the bioavailability of zoledronic acid is improved by at least about 20% as compared to administration of zoledronic acid in the diacid form.
Embodiment 129. The method of embodiment 120, 121, 122, 123, 124, 125, 126, 127, or 128, further comprising administering, on a molar basis, less of the zoledronic acid in the disodium salt form than would be administered of zoledronic acid in the diacid form in order to achieve the same plasma levels of zoledronic acid.
Embodiment 130. The method of embodiment 129, wherein at least about mole% less of the disodium salt form is administered as compared the amount of zoledronic acid in the diacid form that would be administered in order to achieve the same plasma levels of zoledronic acid.
Embodiment 131. The method of embodiment 129, wherein the disodium salt form is administered in an amount, on a molar basis, that has a value of about 0.8nd to about 1.2nd, wherein:
nd = (bdba)(na) Date Recue/Date Received 2021-04-07 wherein ba is the bioavailability of the diacid form, bd is the bioavailability of the disodium salt form, and na is the number of moles of zoledronic acid in the diacid form that would be administered in order to achieve the same plasma levels of zoledronic acid.
Embodiment 132. The method of embodiment 131, wherein the disodium salt is administered in an amount that has a value of about rld.
Embodiment 133. The method of any of embodiments 120-132, wherein the zoledronic acid is used to treat an inflammatory condition.
Embodiment 134. The method of embodiment 133, wherein the zoledronic acid is used to treat arthritis.
Embodiment 135. The method of embodiment 133, wherein the zoledronic acid is used to treat complex regional pain syndrome.
Embodiment 136. The method of any of embodiments 1-39, 62-77, and 120-135, wherein:
a first oral dosage form is administered; and a second oral dosage form is administered;
wherein, with respect to the first oral dosage form, the second oral dosage form is administered at 10 x Tõx or greater, wherein Tõx is the time of maximum plasma concentration for the first oral dosage form.
Embodiment 137. A dosage form comprising zoledronic acid in the disodium salt form, wherein the bioavailability, in a mammal, of zoledronic acid in the disodium salt form is greater than the bioavailability of zoledronic acid in the diacid form would be in the same dosage form.
Embodiment 138. A dosage form comprising zoledronic acid in the disodium salt form, wherein the dosage form contains an amount of zoledronic acid in the disodium salt form that provides an area under the plasma concentration curve of zoledronic acid of about 4 ng=h/mL to about 2000 ng=h/mL to a human being to which the dosage form is administered.
Embodiment 139. The dosage form of embodiment 138, wherein the dosage form contains an amount of zoledronic acid in the disodium salt form that Date Recue/Date Received 2021-04-07 provides an area under the plasma concentration curve of zoledronic acid of about 100 ng=h/mL to about 2000 ng=h/mL to a human being to which the dosage form is administered.
Embodiment 140. The dosage form of embodiment 138, wherein the dosage form contains an amount of zoledronic acid in the disodium salt form that provides an area under the plasma concentration curve of zoledronic acid of about 20 ng=h/mL to about 700 ng=h/mL to a human being to which the dosage form is administered.
Embodiment 141. The dosage form of embodiment 138, wherein the dosage form contains an amount of zoledronic acid in the disodium salt form that provides an area under the plasma concentration curve of zoledronic acid of about 4 ng-h/mL to about 100 ng=h/mL to a human being to which the dosage form is administered.
Embodiment 142. A dosage form comprising zoledronic acid in the disodium salt form, wherein the disodium salt form is present in a lower molar amount than would be present if the zoledronic acid were in the diacid form; and wherein the zoledronic acid in the disodium salt form has an improved bioavailability as compared to the zoledronic acid in the diacid form to the extent that the lower molar amount of the disodium salt in the dosage form does not reduce the amount of zoledronic acid delivered to the plasma of a mammal.
Embodiment 143. The dosage form of embodiment 137, 138, 139, 140, 141, or 142, wherein the dosage form is a solid.
Embodiment 144. The dosage form of embodiment 142 or 143, wherein the bioavailability of zoledronic acid in the disodium salt form is improved by at least about 10% as compared to an otherwise identical dosage form containing zoledronic acid in the diacid form.
Embodiment 145. The dosage form of embodiment 142, 143, or 144, containing at least about 20 mole% less of the disodium salt form as compared to the amount of the zoledronic acid in the diacid form that would be present if the zoledronic acid were in the diacid form.

Date Recue/Date Received 2021-04-07 Embodiment 146. The dosage form of embodiment 142, wherein the disodium salt form is present in an amount, on a molar basis, that has a value of about 0.9nd to about 1.1nd, wherein:
nd = (ba/bd)(nd) wherein ba is the bioavailability of the diacid form, bd is the bioavailability of the disodium salt form, and na is the number of moles of the diacid form that would be present if the zoledronic acid were in the diacid form.
Embodiment 147. The dosage form of embodiment 146, wherein the disodium salt is administered in an amount that has a value of about nd.
Embodiment 148. The method of any of embodiments 1-39, 62-77, and 120-136, wherein:
only a single oral dosage form is administered; or a first oral dosage form is administered, and a second oral dosage form is administered after the first oral dosage form, wherein the second oral dosage form is administered before the maximum pain relieving effect of the first oral dosage form is achieved, or the second oral dosage form is administered before an observable pain relieving effect is achieved.
Embodiment 149. The method of embodiment 148, wherein the second oral dosage form is administered before an observable pain relieving effect is achieved.
Embodiment 150. The method of any of embodiments 1-39, 62-77, and 120-132, wherein a first dosage form is administered, followed by administration of a second dosage form, wherein the second dosage form is administered after the maximum pain relieving effect of the first oral dosage form is achieved, and the second oral dosage form is administered while a pain relieving effect from the first oral dosage form is observable.
Embodiment 151. The method of embodiment 148, 149, or 150, wherein the second oral dosage form is administered about 24 hours to about 28 days after the first oral dosage form is administered.
Embodiment 152. The method of any of embodiments 20-39, wherein the human being is about 30 years old to about 75 years old.

Date Recue/Date Received 2021-04-07 Embodiment 153. The method of any of embodiments 20-39, wherein the human being is about 1 year old to about 16 years old.
Embodiment 154. The method of any of embodiments 20-39, wherein the human being is about 80 years old to about 95 years old.
Embodiment 155. The method of any of embodiments 20-39, wherein the human being has suffered from the arthritis for at least 2 months.
Embodiment 156. The method of any of embodiments 20-39, wherein the arthritis affects, a knee, an elbow, a wrist, a shoulder, or a hip.
Embodiment 157. The method of any of embodiments 1-44, 62-133, and 144-156, wherein the mammal or human being to which the zoledronic acid is administered does not eat food or drink beverage for at least 1 hour before the zoledronic acid is administered.
Embodiment 158. The method of embodiment 157, wherein the mammal or human being to which the zoledronic acid is administered does not eat food or drink beverage for at least 2 hours before the zoledronic acid is administered.
Embodiment 159. The method of embodiment 158, wherein the mammal or human being to which the zoledronic acid is administered does not eat food or drink beverage for at least 4 hours before the zoledronic acid is administered.
Embodiment 160. The method of embodiment 159, wherein the mammal or human being to which the zoledronic acid is administered does not eat food or drink beverage for at least 6 hours before the zoledronic acid is administered.
Embodiment 161. The method of any of embodiments 157-160, wherein the mammal or human being to which the zoledronic acid is administered does not eat food or drink beverage for at least 30 minutes after the zoledronic acid is administered.
Embodiment 162. The method of embodiment 161, wherein the mammal or human being to which the zoledronic acid is administered does not eat food or drink beverage for at least 1 hour after the zoledronic acid is administered.
Embodiment 163. The method of embodiment 161, where in the mammal or human being to which the zoledronic acid is administered does not eat food or drink beverage for at least 2 hours after the zoledronic acid is administered.

Date Recue/Date Received 2021-04-07 Embodiment 164. The method, dosage form, or product, of any preceding embodiment, wherein the zoledronic acid in the oral dosage form has a 24 hour sustained plasma level factor of about 1 or higher.
Embodiment 165. The method, dosage form, or product, of any preceding embodiment, wherein the zoledronic acid in the oral dosage form has a 24 hour sustained plasma level factor that is higher than that of intravenously administered zoledronic acid.
Embodiment 166. The method, dosage form, or product, of any preceding embodiment, wherein the oral dosage form is a solid that has a hardness of about 5 kPa to about 20 kPa.
Embodiment 167. A method of treating bone marrow lesions comprising:
selecting a patient having a bone marrow lesion and OARSI grade 0 of joint space narrowing, and administering an inhibitor of osteoclast activity to the patient for the treatment of the bone marrow lesion.
Embodiment 168. The method of embodiment 167, wherein the inhibitor of osteoclast activity is administered at least twice.
Embodiment 169. The method of embodiment 167, wherein the inhibitor of osteoclast activity is administered about every three months, or more frequently.
Embodiment 170. The method of embodiment 167, wherein the inhibitor of osteoclast activity comprises a nitrogen-containing bisphosphonate.
Embodiment 171. The method of any one of embodiments 167-170, wherein the inhibitor of osteoclast activity is or comprises zoledronic acid.
Embodiment 172. The method of any one of embodiments 167-170, wherein the inhibitor of osteoclast activity is or comprises pamidronic acid.
Embodiment 173. The method of any one of embodiments 167-170, wherein the inhibitor of osteoclast activity is or comprises neridronic acid.
Embodiment 174. The method of any one of embodiments 167-170, wherein the inhibitor of osteoclast activity is or comprises olpadronic acid.
Embodiment 175. The method of any one of embodiments 167-170, wherein the inhibitor of osteoclast activity is or comprises alendronic acid.
Embodiment 176. The method of any one of embodiments 167-170, wherein the inhibitor of osteoclast activity is or comprises incadronic acid.

Date Recue/Date Received 2021-04-07 Embodiment 177. The method of any one of embodiments 167-170, wherein the inhibitor of osteoclast activity is or comprises ibandronic acid.
Embodiment 178. The method of any one of embodiments 167-170, wherein the inhibitor of osteoclast activity is or comprises risedronic acid.
Embodiment 179. The method of any one of embodiments 167-178, wherein the inhibitor of osteoclast activity is administered orally.
Embodiment 180. The method of any one of embodiments 167-178, wherein the inhibitor of osteoclast activity is administered intravenously.
Embodiment 181. The method of any one of embodiments 167-180, wherein the patient experiences a reduction in bone marrow lesion size that is at least about 100% greater than a reduction in bone marrow lesion size achieved with a placebo.
Embodiment 182. The method of any one of embodiments 167-180, wherein the patient experiences a reduction in bone marrow lesion size that is at least about 150% greater than a reduction in bone marrow lesion size achieved with a placebo.
Embodiment 183. The method of any one of embodiments 167-182, wherein the inhibitor of osteoclast activity is administered at least twice over a period of at least four weeks.
Embodiment 184. The method of any one of embodiments 167-183, wherein the inhibitor of osteoclast activity is administered once weekly for a period of six weeks.
Embodiment 185. The method of any one of embodiments 167-184, wherein the inhibitor of osteoclast activity comprises zoledronic acid, and the weekly dose is between about 25 mg and about 75 mg.
Embodiment 186. A method of treating knee pain comprising: selecting a patient having knee pain and OARS! grade 0 of joint space narrowing, and administering an inhibitor of osteoclast activity to the patient for the treatment of the knee pain.
Embodiment 187. The method of embodiment 186, wherein the inhibitor of osteoclast activity is administered at least twice.
Embodiment 188. The method of any one of embodiments 186-187, wherein the inhibitor of osteoclast activity is administered about every three months, or more frequently.
Date Recue/Date Received 2021-04-07 Embodiment 189. The method of any one of embodiments 186-188, wherein the inhibitor of osteoclast activity comprises a nitrogen-containing bisphosphonate.
Embodiment 190. The method of any one of embodiments 186-189, wherein the patient experiences pain relief three months after administration of the inhibitor of osteoclast activity.
Embodiment 191. The method of any one of embodiments 186-190, wherein the inhibitor of osteoclast activity is or comprises zoledronic acid.
Embodiment 192. The method of any one of embodiments 186-190, wherein the inhibitor of osteoclast activity is or comprises pamidronic acid.
Embodiment 193. The method of any one of embodiments 186-190, wherein the inhibitor of osteoclast activity is or comprises neridronic acid.
Embodiment 194. The method of any one of embodiments 186-190, wherein the inhibitor of osteoclast activity is or comprises olpadronic acid.
Embodiment 195. The method of any one of embodiments 186-190, wherein the inhibitor of osteoclast activity is or comprises alendronic acid.
Embodiment 196. The method of any one of embodiments 186-190, wherein the inhibitor of osteoclast activity is or comprises incadronic acid.
Embodiment 197. The method of any one of embodiments 186-190, wherein the inhibitor of osteoclast activity is or comprises ibandronic acid.
Embodiment 198. The method of any one of embodiments 186-190, wherein the inhibitor of osteoclast activity is or comprises risedronic acid.
Embodiment 199. The method of any one of embodiments 186-198, wherein the patient experiences a reduction in pain intensity¨when using a 100 mm visual analog scale¨of at least about 20.
Embodiment 200. A method of treating a bone marrow lesion of the knee comprising: selecting a patient having a bone marrow lesion of the knee and OARS!
Grade 0 or Kellgren and Lawrence Grade 0 or Grade 1 of joint space narrowing, and administering an inhibitor of osteoclast activity to the patient for the treatment of the bone marrow lesion.
Embodiment 201. The method of embodiment 200, wherein the inhibitor of osteoclast activity is administered at least twice.
Embodiment 202. The method of embodiment 201, wherein the inhibitor of osteoclast activity is administered about every three months, or more frequently.

Date Recue/Date Received 2021-04-07 Embodiment 203. The method of embodiment 200, wherein the inhibitor of osteoclast activity comprises a nitrogen-containing bisphosphonate.
Embodiment 204. The method of embodiment 203, wherein the inhibitor of osteoclast activity is zoledronic acid.
Embodiment 205. The method of embodiment 203, wherein the inhibitor of osteoclast activity is pamidronic acid.
Embodiment 206. The method of embodiment 203, wherein the inhibitor of osteoclast activity is neridronic acid.
Embodiment 207. The method of embodiment 203, wherein the inhibitor of osteoclast activity is olpadronic acid.
Embodiment 208. The method of embodiment 203, wherein the inhibitor of osteoclast activity is minodronic acid.
Embodiment 209. The method of embodiment 203, wherein the inhibitor of osteoclast activity is incadronic acid.
Embodiment 210. The method of embodiment 203, wherein the inhibitor of osteoclast activity is ibandronic acid.
Embodiment 211. The method of embodiment 203, wherein the inhibitor of osteoclast activity is risedronic acid.
Embodiment 212. The method of embodiment 203, wherein the inhibitor of osteoclast activity is alendronic acid.
Embodiment 213. The method of embodiment 200, wherein the inhibitor of osteoclast activity is administered orally.
Embodiment 214. The method of embodiment 200, wherein the inhibitor of osteoclast activity is administered intravenously.
Embodiment 215. The method of embodiment 200, wherein the patient experiences a reduction in bone marrow lesion size that is at least about 15%
within about 6 months after the inhibitor of osteoclast activity is administered to the patient.
Embodiment 216. The method of embodiment 200, wherein the patient experiences a reduction in bone marrow lesion size that is at least about 25%
within about 6 months after the inhibitor of osteoclast activity is administered to the patient.
Embodiment 217. The method of embodiment 201, wherein the inhibitor of osteoclast activity is administered at least twice over a period of at least four weeks.
Embodiment 218. The method of embodiment 201, wherein the inhibitor of osteoclast activity is administered once weekly for a period of six weeks.

Date Recue/Date Received 2021-04-07 Embodiment 219. The method of embodiment 218, wherein the inhibitor of osteoclast activity comprises zoledronic acid, and the weekly dose is between about 25 mg and about 75 mg.
Embodiment 220. A method of treating knee pain comprising:
a. selecting a patient having knee pain, and:
i. OARSI Grade 0 or Kellgren and Lawrence Grade 0 or Grade 1 of joint space narrowing, or ii. pain intensity of 5 or greater measured using the 0-10 NRS or 5 cm or greater using the 10 cm VAS; and b. administering an inhibitor of osteoclast activity to the patient.
Embodiment 221. The method of embodiment 220, comprising selecting a patient having OARS! Grade 0 or Kellgren and Lawrence Grade 0 or Grade 1 of joint space narrowing.
Embodiment 222. The method of embodiment 220 or 221, comprising selecting a patient having pain intensity of 5 or greater measured using the 0-NRS or 5 cm or greater using the 10 cm VAS.
Embodiment 223. The method of embodiment 220, wherein the inhibitor of osteoclast activity is administered at least twice.
Embodiment 224. The method of embodiment 223, wherein the inhibitor of osteoclast activity is administered about every three months, or more frequently.
Embodiment 225. The method of embodiment 220, wherein the inhibitor of osteoclast activity comprises a nitrogen-containing bisphosphonate.
Embodiment 226. The method of embodiment 220, wherein the patient experiences pain relief within about three months after the inhibitor of osteoclast activity is administered to the patient.
Embodiment 227. The method of embodiment 226, wherein the patient experiences pain relief at least 24 hours after the inhibitor of osteoclast activity is administered to the patient.
Embodiment 228. The method of embodiment 220, wherein the inhibitor of osteoclast activity is zoledronic acid.
Embodiment 229. The method of embodiment 220, wherein the inhibitor of osteoclast activity is minodronic acid.
Embodiment 230. The method of embodiment 220, wherein the inhibitor of osteoclast activity is neridronic acid.

Date Recue/Date Received 2021-04-07 Embodiment 231. The method of embodiment 220, wherein the inhibitor of osteoclast activity is olpadronic acid.
Embodiment 232. The method of embodiment 220, wherein the inhibitor of osteoclast activity is alendronic acid.
Embodiment 233. The method of embodiment 220, wherein the inhibitor of osteoclast activity is incadronic acid.
Embodiment 234. The method of embodiment 220, wherein the inhibitor of osteoclast activity is ibandronic acid.
Embodiment 235. The method of embodiment 220, wherein the inhibitor of osteoclast activity is risedronic acid.
Embodiment 236. The method of embodiment 220, wherein the patient experiences a reduction in pain intensity¨when using a 100 mm visual analog scale¨of at least about 5.
Embodiment 237. The method of embodiment 220, wherein the inhibitor of osteoclast activity is administered at least twice over a period of at least four weeks.
Embodiment 238. The method of embodiment 220, wherein the inhibitor of osteoclast activity is administered once weekly for a period of six weeks.
Embodiment 239. The method of embodiment 238, wherein the inhibitor of osteoclast activity comprises zoledronic acid, and the weekly dose is between about 25 mg and about 75 mg.
Embodiment 240. A method of treating moderate to severe knee pain comprising administering an inhibitor of osteoclast activity to a person suffering from moderate to severe knee pain.
Embodiment 241. The method of embodiment 240, wherein the person suffering from moderate to severe knee pain has a normal joint space in the knee.
Embodiment 242. The method of embodiment 240, wherein the inhibitor of osteoclast activity is administered at least twice.
Embodiment 243. The method of embodiment 240, wherein the inhibitor of osteoclast activity is administered about every three months, or more frequently.
Embodiment 244. The method of embodiment 240, wherein the inhibitor of osteoclast activity comprises a nitrogen-containing bisphosphonate.
Embodiment 245. The method of embodiment 240, wherein the patient experiences pain relief within about three months after the inhibitor of osteoclast activity is administered to the patient.

Date Recue/Date Received 2021-04-07 Embodiment 246. The method of embodiment 245, wherein the patient experiences pain relief at least 24 hours after the inhibitor of osteoclast activity is administered to the patient.
Embodiment 247. The method of embodiment 240, wherein the inhibitor of osteoclast activity is zoledronic acid.
Embodiment 248. The method of embodiment 240, wherein the inhibitor of osteoclast activity is minodronic acid.
Embodiment 249. The method of embodiment 240, wherein the inhibitor of osteoclast activity is neridronic acid.
Embodiment 250. The method of embodiment 240, wherein the inhibitor of osteoclast activity is olpadronic acid.
Embodiment 251. The method of embodiment 240, wherein the inhibitor of osteoclast activity is alendronic acid.
Embodiment 252. The method of embodiment 240, wherein the inhibitor of osteoclast activity is incadronic acid.
Embodiment 253. The method of embodiment 240, wherein the inhibitor of osteoclast activity is ibandronic acid.
Embodiment 254. The method of embodiment 240, wherein the inhibitor of osteoclast activity is risedronic acid.
Embodiment 255. The method of embodiment 240, wherein the patient experiences a reduction in pain intensity¨when using a 100 mm visual analog scale¨of at least about 5.
Embodiment 256. The method of embodiment 240, wherein the inhibitor of osteoclast activity is administered at least twice over a period of at least four weeks.
Embodiment 257. The method of embodiment 240, wherein the inhibitor of osteoclast activity is administered once weekly for a period of six weeks.
Embodiment 258. The method of embodiment 257, wherein the inhibitor of osteoclast activity comprises zoledronic acid, and the weekly dose is between about 25 mg and about 75 mg.
Embodiment 259. A method of safely delivering zoledronic acid to the blood of a mammal through repeated oral administration comprising:
Date Recue/Date Received 2021-04-07 orally administering about 0.4 mg/kg to about 4 mg/kg of zoledronic acid to the mammal no more frequently than once a day and more frequently than once a week; or orally administering about 0.4 mg/kg to about 10 mg/kg to the mammal once a week, or less frequently.
Embodiment 260. The method of any preceding embodiment, such as embodiment 259, wherein about 0.5 mg/kg to about 2 mg/kg is orally administered to the mammal daily.
Embodiment 261. The method of any preceding embodiment, such as embodiment 260, wherein about 0.6 mg/kg to about 0.9 mg/kg is orally administered to the mammal daily.
Embodiment 262. The method of any preceding embodiment, such as embodiment 259, wherein about 0.5 mg/kg to about 2 mg/kg is orally administered to the mammal weekly.
Embodiment 263. The method of any preceding embodiment, such as embodiment 263, wherein about 0.6 mg/kg to about 0.9 mg/kg is orally administered to the mammal weekly.
Embodiment 264. The method of any preceding embodiment, such as embodiment 259, 260, 261, 262, or 263, wherein zoledronic acid is orally administered about 3 to about 10 times.
Embodiment 265. The method of any preceding embodiment, such as embodiment 259, 260, 261, 262, 263, or 264, wherein zoledronic acid is orally administered in a dosage form comprising more than about 10% zoledronic acid by weight.
Embodiment 266. The method of any preceding embodiment, such as embodiment 259, 260, 261, 262, 263, 264, or 265, wherein zoledronic acid is administered in a manner and amount that results in the mammal having an AUC0-of zoledronic acid that is about 50 ng=h/mL to about 500 ng=h/mL with each administration of zoledronic acid.
Embodiment 267. The method of any preceding embodiment, such as embodiment 266, wherein zoledronic acid is administered in a manner and amount that results in the mammal having an AUC0_24 of zoledronic acid that is about ng=h/mL to about 500 ng=h/mL with each administration of zoledronic acid.

Date Recue/Date Received 2021-04-07 Embodiment 268. A method of preparing an oral dosage form that is safe for repeated administration to a mammal comprising combining zoledronic acid with an excipient that is pharmaceutically acceptable to the mammal, wherein the amount of zoledronic acid that is combined with the excipient is such that zoledronic acid is present in the oral dosage form in an amount that is 0.4 mg/kg to about 10 mg/kg based upon the weight of the mammal.
Embodiment 269. The method of any preceding embodiment, such as embodiment 268, wherein the amount of zoledronic acid that is combined with the excipient is such that the oral dosage form comprises more than about 10%
zoledronic acid by weight.
Embodiment 270. The method of any preceding embodiment, such as embodiment 268 or 269, wherein the amount of zoledronic acid that is combined with the excipient is such that zoledronic acid is present in the oral dosage form in an amount that is 0.4 mg/kg to about 3 mg/kg based upon the weight of the mammal.
Embodiment 271. The method of any preceding embodiment, such as embodiment 270, wherein the amount of zoledronic acid that is combined with the excipient is such that zoledronic acid is present in the oral dosage form in an amount that is 0.4 mg/kg to about 1.5 mg/kg based upon the weight of the mammal.
Embodiment 272. The method of any preceding embodiment, such as embodiment 270, wherein the amount of zoledronic acid that is combined with the excipient is such that zoledronic acid is present in the oral dosage form in an amount that is 0.6 mg/kg to about 0.9 mg/kg based upon the weight of the mammal.
Embodiment 273. The method of any preceding embodiment, such as embodiment 268, 269, 270, 271, or 272, wherein the oral dosage form is safe for once daily administration of the oral dosage form for about 3 to about 10 days.
Embodiment 274. The method of any preceding embodiment, such as embodiment 268, 269, 270, 271, or 272, wherein the oral dosage form is safe for once weekly administration of the oral dosage form for about 3 to about 10 weeks.
Embodiment 275. A method of safely delivering zoledronic acid to the blood of a mammal through repeated oral administration comprising:
orally administering about 0.05 mg/kg to about 4 mg/kg of zoledronic acid to the mammal no more frequently than once a day and more frequently than once a week; or Date Recue/Date Received 2021-04-07 orally administering about 0.1 mg/kg to about 10 mg/kg to the mammal once a week, or less frequently wherein zoledronic acid is orally administered at least 5 times.
Embodiment 276. The method of any preceding embodiment, such as embodiment 275, wherein zoledronic acid is orally administered about 5 to about 10 times.
Embodiment 277. The method of any preceding embodiment, such as embodiment 275 or 276, wherein zoledronic acid is orally administered in a dosage form comprising more than about 10% zoledronic acid by weight.
Embodiment 278. The method of any preceding embodiment, such as embodiment 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, or 277, wherein the mammal is a human being.
Embodiment 279. The method of any preceding embodiment, such as embodiment 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, or 278, wherein about 50 mg to about 350 mg of oral zoledronic acid is administered to the mammal per month.
Embodiment 280. An oral dosage form prepared by the method of any preceding embodiment, such as embodiment 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, or 279.

[0270] Unless otherwise indicated, all numbers expressing quantities of ingredients, properties such as molecular weight, reaction conditions, and so forth used in the specification and claims are to be understood in all instances as indicating both the exact values as shown and as being modified by the term "about."
Accordingly, unless indicated to the contrary, the numerical parameters set forth in the specification and attached claims are approximations that may vary depending upon the desired properties sought to be obtained. At the very least, and not as an attempt to limit the application of the doctrine of equivalents to the scope of the claims, each numerical parameter should at least be construed in light of the number of reported significant digits and by applying ordinary rounding techniques.

[0271] The terms "a," "an," "the" and similar referents used in the context of describing the invention (especially in the context of the following claims) are to be construed to cover both the singular and the plural, unless otherwise indicated herein or clearly contradicted by context. All methods described herein can be performed in Date Recue/Date Received 2021-04-07 any suitable order unless otherwise indicated herein or otherwise clearly contradicted by context. The use of any and all examples, or exemplary language (e.g., "such as") provided herein is intended merely to better illuminate the invention and does not pose a limitation on the scope of any claim. No language in the specification should be construed as indicating any non-claimed element essential to the practice of the invention.

[0272] Groupings of alternative elements or embodiments disclosed herein are not to be construed as limitations. Each group member may be referred to and claimed individually or in any combination with other members of the group or other elements found herein. It is anticipated that one or more members of a group may be included in, or deleted from, a group for reasons of convenience and/or patentability. When any such inclusion or deletion occurs, the specification is deemed to contain the group as modified thus fulfilling the written description of all Markush groups used in the appended claims.

[0273] Certain embodiments are described herein, including the best mode known to the inventors for carrying out the invention. Of course, variations on these described embodiments will become apparent to those of ordinary skill in the art upon reading the foregoing description. The inventor expects skilled artisans to employ such variations as appropriate, and the inventors intend for the invention to be practiced otherwise than specifically described herein. Accordingly, the claims include all modifications and equivalents of the subject matter recited in the claims as permitted by applicable law. Moreover, any combination of the above-described elements in all possible variations thereof is contemplated unless otherwise indicated herein or otherwise clearly contradicted by context.

[0274] In closing, it is to be understood that the embodiments disclosed herein are illustrative of the principles of the claims. Other modifications that may be employed are within the scope of the claims. Thus, by way of example, but not of limitation, alternative embodiments may be utilized in accordance with the teachings herein. Accordingly, the claims are not limited to embodiments precisely as shown and described.

Date Recue/Date Received 2021-04-07

Claims (35)

CLAIMS:

1. Use of a compound for the treatment of knee pain, in a mammal in need thereof, wherein the mammal has pain intensity of 5 cm or greater measured using the 0-10 cm visual analog scale (VAS), and an OARSI Grade 0 or Kellgren and Lawrence Grade 0 or Grade 1 of joint space narrowing; and wherein the compound is a bisphosphonate.

2. Use of a compound in the manufacture of a medicament for the treatment of knee pain, in a mammal in need thereof, wherein the mammal has pain intensity of 5 cm or greater measured using the 0-10 cm VAS, and an OARSI Grade 0 or Kellgren and Lawrence Grade 0 or Grade 1 of joint space narrowing; and wherein the compound is a bisphosphonate.

3. The use of claim 1 or 2, wherein the mammal has a bone marrow lesion.

4. The use of claim 3, wherein the mammal experiences a reduction in bone marrow lesion size that is at least 15% within about 6 months after commencement of treatment.

5. The use of any one of claims 1 to 4, wherein the compound is adapted for administration at least twice.

6. The use of any one of claims 1 to 5, wherein the compound is adapted for administration about every three months, or more frequently.

7. The use of any one of claims 1 to 6, wherein the compound is adapted for oral administration.

Date Recue/Date Received 2021-04-07

8. The use of any one of claims 1 to 7, wherein the compound is adapted for administration intravenously.

9. The use of any one of claims 1 to 8, wherein the mammal has pain intensity of 6 cm or greater measured using the 0-10 cm VAS.

10. The use of any one of claims 1 to 9, wherein the mammal has pain intensity of 7 cm or greater measured using the 0-10 cm VAS.

11. The use of any one of claims 1 to 10, wherein the mammal experiences a reduction in pain intensity of at least 0.5 cm when using the 0-10 cm VAS.

12. The use of any one of claims 1 to 11, wherein the compound is zoledronic acid.

13. The use of claim 12, wherein the zoledronic acid is adapted for oral administration to the mammal in a manner that results in a bioavailability of 1.2%
to 3% in the mammal.

14. The use of claim 12 or 13, wherein the zoledronic acid is adapted for administration once weekly for a period of six weeks.

15. The use of claim 14, wherein the zoledronic acid is adapted for administration as a once weekly dose of about 25 mg to about 75 mg.

16. The use of any one of claims 12 to 15, wherein the zoledronic acid is in a disodium salt form.

17. The use of any one of claims 1 to 11, wherein the compound is neridronic acid.

18. The use of any one of claims 1 to 17, wherein the mammal is a human being.

19. A compound for use in the treatment of knee pain in a mammal in need thereof, Date Recue/Date Received 2021-04-07 wherein the mammal has pain intensity of 5 cm or greater measured using the 0-10 cm VAS, and an OARSI Grade 0 or Kellgren and Lawrence Grade 0 or Grade 1 of joint space narrowing; and wherein the compound is a bisphosphonate.

20. The compound for use of claim 19, wherein the mammal has a bone marrow lesion.

21. The compound for use of claim 20, wherein the mammal experiences a reduction in bone marrow lesion size that is at least 15% within about 6 months after commencement of treatment.

22. The compound for use of any one of claims 19 to 21, wherein the compound is adapted for administration at least twice.

23. The compound for use of any one of claims 19 to 22, wherein the compound is adapted for administration about every three months, or more frequently.

24. The compound for use of any one of claims 19 to 23, wherein the compound is adapted for oral administration.

25. The compound for use of any one of claims 19 to 23, wherein the compound is adapted for intravenous administration.

26. The compound for use of any one of claims 19 to 25, wherein the mammal has pain intensity of 6 cm or greater measured using the 0-10 cm VAS.

27. The compound for use of any one of claims 19 to 26, wherein the mammal has pain intensity of 7 cm or greater measured using the 0-10 cm VAS.

28. The compound for use of any one of claims 19 to 27, wherein the mammal experiences a reduction in pain intensity of at least 0.5 cm when using the 0-10 cm VAS.

Date Recue/Date Received 2021-04-07

29. The compound for use of any one of claims 19 to 28, wherein the compound is zoledronic acid.

30. The compound for use of claim 29, wherein the zoledronic acid is adapted for oral administration to the mammal in a manner that results in a bioavailability of 1.2% to 3% in the mammal.

31. The compound for use of claim 29 or 30, wherein the zoledronic acid is adapted for administration once weekly for a period of six weeks.

32. The compound for use of claim 31, wherein the zoledronic acid is adapted for administration as a once weekly dose of about 25 mg to about 75 mg.

33. The compound for use of any one of claims 29 to 32, wherein the zoledronic acid is in a disodium salt form.

34. The compound for use of any one of claims 19 to 28, wherein the compound is neridronic acid.

35. The compound for use of any one of claims 19 to 34, wherein the mammal is a human being.

Date Recue/Date Received 2021-04-07