CA2897354C - A hollow fiber module having thin film composite- aquaporin modified membranes - Google Patents

Abstract

The present invention relates to a hollow fiber (HF) module having fibers modified with a thin film composite (TFC) layer comprising aquaporin water channels.

Description

A HOLLOW FIBER MODULE HAVING THIN FILM COMPOSITE-AQUAPORIN MODIFIED MEMBRANES
FIELD OF THE INVENTION
The present invention relates to a hollow fiber (HF) module having thin film composite (TFC) modified fiber membranes, such as having a polyamide TFC layer on the outside or inside of the fibers, or on both sides, and comprising aquaporin water channels in said TFC layer. The present invention also relates to a hollow fiber membrane having a thin film composite (TFC) modified separation layer comprising aquaporin water channels, such as having a polyamide TFC layer on the outside or inside of the fibers and to a method of preparing said TFC modification directly on the fibers as mounted in the module and where said TFC layer comprises aquaporin water channels immobilised or embedded in said layer.
BACKGROUND OF THE INVENTION
Recently, it has been shown how thin film composite hollow fiber membranes can be prepared for forward osmosis (Sukitpaneenit & Chung 2012) through interfacial polymerization creating a polyamide thin layer on the inner surface of PES
hollow fiber support membranes where a 2 wt % of meta-phenylene diamine (MPD) aqueous solution containing 0.5 wt % of triethylamine and 0.1 wt % of sodium dodecyl sulfate is introduced through pumping from a bottom inlet into a vertically positioned HF
module followed by air drying and then introduction of a 0.15 wt % of trimesoyl chloride (TMC) in hexane solution to form the polyamide thin film and finally purging with air and curing at 65 C, rinsing with deionized water and storing in deionized water.
Furthermore, Peinemann et al. describes a method of preparing a similar TFC layer on the interior surface of a hollow fiber, cf. US published patent application No.
2007/0199892.
Moreover, Zhong et al. (2013) describe the development of TFC forward osmosis hollow fiber membranes using direct sulfonated polyphenylenesulfone as membrane substrate.
However, for a wide range of separation applications where hollow fiber modules are employed it is of importance to be able to separate or filter out organic solutes of relatively low molecular weight during the filtration process. For example, in haemodialysis where HF modules are widely used, a uremic toxin and organic degradation product, such as indoxyl sulfate (Indoxyl sulfate potassium salt, CAS
Number 2642-37-7, molecular weight 251.30) poses a particularly difficult problem.
Indoxyl sulfate is accumulated in the serum of chronic kidney disease patients. A part of , the dietary protein-derived tryptophan is metabolized into indole by tryptophanase in intestinal bacteria. Indole is absorbed into the blood from the intestine, and is metabolized to indoxyl sulfate in the liver. Indoxyl sulfate is normally excreted into urine. In haemodialysis patients, however, an inadequate renal clearance of indoxyl sulfate leads to its elevated serum levels, cf. Niwa T. (2010). Niwa et al.
(1997) have advanced the hypothesis that accumulation of indoxyl sulfate accelerates glomerular sclerosis and progression of kidney disease. Administration of an oral adsorbent lowers indoxyl sulfate levels in undialyzed uraemic patients, cf Niwa et al. (1997).
Current dialysis methods, i.e. haemodialysis and peritoneal dialysis, the latter being characterised by continuous ultrafiltration and solute removal, do not sufficiently remove some low molecular weight degradation products from serum, such as indoxyl sulfate and p-cresol (4-methylphenol, CAS No. 106-44-5, molecular weight 108.13). In addition, small water-soluble molecules, such as urea, uric acid and creatinine, and peptides/proteins, such as f32-microglobulin should preferably be removed during dialysis. A direct association between p-cresol, mainly reflecting p-cresyl sulfate, and overall mortality and cardiovascular disease in end-stage renal disease and in chronic kidney disease has been found. Likewise, direct associations between indoxyl sulfate and overall mortality and cardiovascular disease has been reported. In continuous hemofiltration therapy loss of physiological (vital) proteins should be minimized and removal of low (< 500 Da) and middle molecular weight (from about 500 to about 40 kDa) uremic toxins and peptides should be optimized, cf. Wenhao Xie (2011).
Aoike (2011) mentions the most important features or quality parameters for high performance membranes for use in hemopurification therapies, i.e., high water permeability, capability to remove a wide range of uremic toxins and other characteristic features. However, Aoike also points out that large pore size of existing high performance membranes (HPM) will likely allow blood to be contaminated by the dialysis fluid, because HPMs, such as polyacrylonitrile (PAN) membranes, having a large pore size allow untoward rapid diffusion of dialyzed endotoxin fragments back into the blood compartment.
Accordingly, it remains a problem in the art in providing hollow fiber modules that are able to separate low molecular weight compounds, enabling their removal from liquids in

2 processes such as haemodialysis and in which the hollow fibre module combine a high water permeability with smaller pore sizes.
SUMMARY OF THE INVENTION
Broadly, the present invention provides a hollow fiber module which is able to separate low molecular weight compounds, for example enabling the HF module to concentrate organic compounds, including urea, indoxyl sulfate, p-cresol and/or p-cresyl sulfate and preferably compounds having a molecular weight of less than about 500 Da and thus enable improved removal of said compounds, e.g. from a liquid. Alternatively or additionally, the present invention aims to provide a hollow fiber module having high water permeability but with smaller pore sizes, such as having a pore diameter of less than about 5 to 10 nm, cf. Clark & Gao (2002).
Accordingly, in one aspect, the present invention provides a hollow fiber (HF) module having a TFC layer comprising aquaporin water channels formed on its fiber surfaces, such as the inner fiber surface (lumen).
In a further aspect, the present invention provides a method of preparing a hollow fiber module comprising HF membranes modified with a thin film composite (TFC) layer comprising aquaporin water channels, said method comprising the steps of:
a) obtaining an aquaporin vesicles suspension (proteoliposomes or proteopolymersomes) having from about 25 to about 500 LPR/POPR of protein, b) preparing an aqueous solution of a di- or triamine, c) dissolving a di- or triacyl halide in an apolar organic solvent, d) preparing a mixture of amine and aquaporin vesicle by dissolving/mixing the vesicles preparation from step a) with the solution from step b), e) pumping the mixture from step d) through the lumen of the hollow fibers in a hollow fiber module using its end inlet, 0 removing excess aqueous solution by a gas purging of the lumen side of the fibers using a module inlet, g) injecting the acyl halide solution from step c) into the module through the lumen of the hollow fibers to allow an interfacial polymerization reaction to take place, and h) rinsing the module with an aqueous solvent by injection through a module inlet.

3 In a further aspect, the present invention provides a method of preparing a hollow fiber module comprising HF membranes modified with a thin film composite (TFC) layer comprising aquaporin water channels, said method comprising the steps of a) obtaining an aquaporin vesicles suspension (proteoliposomes or proteopolymersomes) having from about 25 to about 500 LPR/POPR of protein, b) preparing an aqueous solution of 1,3-diaminobenzene of about 1% to about 5 %
(w/w) concentration, c) dissolving benzene-1,3,5-tricarbonyl chloride in an organic solvent selected from the group consisting of hexane, heptane, octane or a mixture of solvents to obtain a .. concentration of about 0.05% to about 1% (w/v), d) preparing an 1,3-diaminobenzene/aquaporin vesicle mixture by dissolving/mixing the vesicles preparation from step a) with the solution from step b), e) pumping the mixture from step d) through the lumen of the hollow fibers in a hollow fiber module using its end inlet, f) removing excess aqueous solution by a gas purging of the lumen side of the fibers using a module inlet, g) injecting the benzene-1,3,5-tricarbonyl chloride solution from step c) into the module through the lumen of the hollow fibers to allow an interfacial polymerization reaction to take place, and h) rinsing the module with an aqueous solvent by injection through a module inlet.
In a further aspect, the present invention provides a method of outside coating a hollow fiber membrane with a thin film composite (TFC) layer comprising aquaporin water channels, said method comprising the steps of:
a) obtaining an aquaporin vesicles suspension (proteoliposomes or proteopolymersomes), b) preparing an aqueous solution of a di- or triamine, c) dissolving a di- or triacyl halide in an apolar organic solvent, d) preparing a mixture of amine and aquaporin vesicle by dissolving/mixing the vesicles preparation from step a) with the solution from step b), e) passing the hollow fiber membrane through the mixture from step d), f) removing excess aqueous solution, g) passing the hollow fiber membrane through the acyl halide solution from step c) to allow an interfacial polymerization reaction to take place, and

4 h) rinsing the hollow fiber membrane with an aqueous solvent, e.g. by passing the hollow fibre through a water bath.
Moreover, the invention relates to a hollow fiber membrane modified with a thin film composite (TFC) layer comprising aquaporin water channels.
In a further aspect, the present invention provides the use of into a hollow fiber (HF) module as described herein for extraction of pure water through forward osmosis or for re-extraction of pure water from a patient's plasma lost through hemodialysis In a further aspect, the present invention provides a method of extracting water from an aqueous liquid comprising the following steps:
a) placing a hollow fiber (HF) module of any one of claims 1 to 9 which is in controlled connection with a first aqueous liquid (feed solution) having an osmotic pressure which is lower than or equal to that of the liquid membrane matrix, and which is further in controlled connection with a second aqueous liquid (draw solution) having an osmotic pressure which is higher than that of the matrix to create an osmotic pressure potential between said first and said second liquid, b) allowing the matrix to absorb pure water from said first liquid and to mediate a pure water flux into said second liquid as long as an osmotic pressure gradient exists, c) optionally separating the extracted pure water from said second liquid.
Embodiments of the present invention will now be described by way of example and not limitation with reference to the accompanying figures. However various further aspects and embodiments of the present invention will be apparent to those skilled in the art in view of the present disclosure.
"and/or" where used herein is to be taken as specific disclosure of each of the two specified features or components with or without the other. For example "A
and/or B" is to be taken as specific disclosure of each of (i) A, (ii) B and (iii) A and B, just as if each is set out individually herein.
Unless context dictates otherwise, the descriptions and definitions of the features set out above are not limited to any particular aspect or embodiment of the invention and apply

5 equally to all aspects and embodiments which are described.
BRIEF DESCRIPTION OF THE DRAWINGS
Fig. lA shows as a photograph and a drawing a HF module having 9 hollow fibers, two end inlets/outlets (1, 2) and two side inlets/outlets (3, 4) as well as four end caps (5) to seal said inlets/outlets. Total length of the module: 100mm; diameter at fibers: 10 mm;
diameter at sealing: 130mm.
Fig. 1B shows as a photograph and a drawing a HF module having 984 fibers, two end inlets/outlets (1, 2) and two side inlets/outlets (3, 4). Total length: 25cm, fiber diameter of 700 Am.
Fig. 2 shows as a photograph and a drawing a cross section of part of a hollow fiber, UltraPESTM, having a wall thickness of about 220 gm 15 gm, an inner diameter of about 760 inn 30 pm, a molecular weight cut-off (MWCO, dextran, 90%, 0 bar) of 65 kD 20 kD, and a transmembrane water flow of>0.65 m1/[min x cm2 x bar] at 25 C.
Picture obtained from MembranaTM GmbH.
Fig. 3 shows a principle sketch of the microstructure of the thin film composite layer formed on a hollow fiber of the invention, cf. Example 1 below.
Fig. 4 shows a principle sketch of a process for automatized continuous outside coating of hollow fibers.
DETAILED DESCRIPTION
More specifically, the present invention relates to a HF module having polyethersulfone (PES) fibers or fibers of other suitable porous support material, such as polysulfone, polyphenylene sulfone, polyether imide, polyvinylpyrrolidone and polyacrylonitrile including blends and mixtures thereof, which has been modified by forming a thin film composite layer, e.g. through interfacial polymerization. In addition, various doping materials may be used when manufacturing the hollow fiber support materials, cf. e.g.
Qian Yang et al. (2009). Such HF modules are commonly used in food and beverage applications such as filtering beer and wine, but also in some water and wastewater applications including wastewater reuse and pool water recycling. For instance, the

6 = German company MembranaTM supplies a hollow fiber module containing several thousands of fibers with an overall surface area of 75 square meters per module. Smaller modules with typically 1 -2 square meters and around 8,000 to 20,000 fibers are commonly used in medical dialysis applications (Fresenius Medical CareTM, GambroTm).
.. In principle, all these commercial products can be coated through interfacial polymerization using the method of the invention resulting in a thin film composite layer wherein aquaporin water channels are incorporated, preferably during its formation, such as by adding a suitable protein suspension or solution, preferably in vesicle form, to the aqueous reactive amine solution, e.g. a meta-phenylene diamine solution, and pumping or injecting the combined solution through the support fibers, removing excess solution and subsequently pumping or injecting a reactive acyl chloride in organic solvent, e.g.
trimesoyl chloride in hexane, and finally rinsing with deionized water, e.g.
MiIliQTM
water. The housing material of the HF modules of the invention can be any suitable material commonly used for HF modules, such as polypropylene, polyethylene, PVDF
and stainless steel. The fibers may be sealed into the HF module housing using commonly known epoxy adhesive materials and the like. Additional examples of HF modules that may be TFC modified according to the invention are found on websites of membrane producers.

In one embodiment of the present invention, the HF module is operated with counter-current flow of draw and feed solutions. In another embodiment of the present invention the HF module is operated with co-current flow of draw and feed solutions. In a further embodiment of the invention the HF module is operated with the draw solution against the TFC layer. In a still further embodiment of the invention the HF module is operated with the feed solution against the TFC layer. In addition, the modified hollow fibres of

7 the invention may be mounted in a system or module for use as a membrane bio-reactor (MBR), where the MBR module containing the hollow fibres is submerged in an aqueous liquid and where purified water is removed from said liquid through the fibres by the use of a draw solution or by application of a moderate suction pressure.
A further aspect of the invention relates to a method of making a hollow fiber membrane modified with a thin film composite (TFC) layer comprising aquaporin water channels, where the TFC layer comprising aquaporin water channels is prepared directly on the surface of membrane fibers mounted and sealed in a HF module, said method comprising the steps of:
a) obtaining an aquaporin vesicles suspension (proteoliposomes or proteopolymersomes) having from about 25 to about 500 LPR/POPR (ratio based on mole content) (preferably 100 LPR/POPR for lipid/diblock copolymer, 50 POPR
for triblock copolymer) of protein, b) preparing an aqueous solution of a diamine, such as 1,3-diaminobenzene, to obtain a solution of about 1% to about 5 %, such as about 2.5 to 4.2% (w/w) concentration, c) dissolving an acyl chloride, such as benzene-1,3,5-tricarbonyl chloride in a hydrocarbon solvent, such as hexane, heptane, octane, nonane having a straight or branched hydrocarbon chain to or mixtures of these, such as isoalkane hydrocarbon solvent obtain a concentration of about 0.05% to about 1 %, such as about 0.15% (w/v), d) preparing a diamine /aquaporin vesicle mixture, such as1,3-diaminobenzene/aquaporin vesicle mixture by dissolving/mixing the vesicles preparation from step a) with the solution from step b), e) pumping the mixture from step d) through the lumen of the hollow fibers in a hollow fiber module using its end inlet, f) removing excess diamine by a gas purging of the lumen side of the fibers using a module inlet, g) injecting the acyl chloride solution from step c) into the module through an inlet to allow an interfacial polymerization reaction to take place, and h) rinsing the module with an aqueous solvent by injection through a module inlet.
Optionally, after filling with water the module is sealed with tight caps to prevent it from drying out.

8 A further aspect of the invention relates to a method of outside coating a hollow fiber membrane with a thin film composite (TFC) layer comprising aquaporin water channels, said method comprising the steps of:
a) obtaining an aquaporin vesicles suspension (proteoliposomes or proteopolymersomes), b) preparing an aqueous solution of a di- or triamine, c) dissolving a di- or triacyl halide in an apolar organic solvent, d) preparing a mixture of amine and aquaporin vesicle by dissolving/mixing the vesicles preparation from step a) with the solution from step b), e) passing the hollow fiber membrane through the mixture from step d), f) removing excess aqueous solution, g) passing the hollow fiber membrane through the acyl halide solution from step c) to allow an interfacial polymerization reaction to take place, and h) rinsing the hollow fiber membrane with an aqueous solvent, e.g. by passing the hollow fibre through a water bath.
In the above described methods of coating the hollow fiber membrane and preparing the hollow fiber module, the steps a)-c) may be performed in any convenient order.
Also the step c) may be performed before or after step d).
In exemplary embodiments, said liposomes used in the preparation of the HF
module of the invention are prepared from lipids such as DPhPC, DOPC, mixed soy bean lipids, such as asolectin and soy lecithin, or E. coli mixed lipids; and said polymersomes may comprise triblock copolymers of the hydrophile-hydrophobe-hydrophile (A-B-A or A-B-C) type or diblock copolymers of the hydrophile-hydrophobe type (A-B).
In a further embodiment said polymersomes may comprise a combination of triblock copolymers of the hydrophile-hydrophobe-hydrophile type and diblock copolymers of the hydrophile-hydrophobe type. In the combinations the diblock copolymers may comprise from about 10 mole % to about 60 mole %, such as from about 25 mole % to about mole %.

9 Said aquaporin water channels are preferably AqpZ channels, but, in principle, all water selective aquaporins, e.g. such as aquaporin Z (AqpZ), Aqpl, GlpF or SoPIP2;1, are useful in the invention.
Said TFC layer is preferably formed through interfacial polymerization of an aqueous solution of an amine with a solution of an acid chloride in an organic solvent, and wherein the aquaporin water channel vesicles are incorporated in said aqueous solution.
Said aquaporin water channels are preferably incorporated in vesicles before incorporation into the TFC layer of the hollow fiber membrane of the invention, and said vesicles may be in the form of liposomes or polymersomes, where said liposomes are prepared from lipids such as DPhPC, DOPC, mixed soy bean lipids, or E. coli mixed lipids, and said polymersomes comprise triblock copolymers of the hydrophile-hydrophobe-hydrophile (A-B-A or A-B-C) type or diblock copolymers of the hydrophile-hydrophobe type (A-B). In a further embodiment said polymersomes may comprise a combination of triblock copolymers of the hydrophile-hydrophobe-hydrophile type and diblock copolymers of the hydrophile-hydrophobe type.
The HF membrane of the invention has preferably a TFC layer formed through interfacial polymerization of an aqueous solution of an amine with a solution of an acid chloride in an organic solvent, and wherein the aquaporin water channel vesicles are incorporated in said aqueous solution.
In one embodiment of the method of preparing the HF module of the invention the fibers are gas purged to blow off excess water in step f) and the module is held upside down in step f).
In a further aspect of the present invention, a hollow fiber module having high water permeability and small pore sizes, such as having a pore diameter of less than about 5 to

10 nm, such as equal to or less than about 1 nm, is provided. Formation of a separation layer in the form of a thin film layer as known in the art onto the surface of a support membrane fiber results in changes to the water transport mechanism. Instead of water transport taking place by normal diffusion through the pores of the support membrane, another type of water transport takes place through the thin film layer as is known from this type of reverse osmosis membranes, where membrane permeability is limited. The nonporous nature of the thin film separating layer results in transport of water requiring "jump diffusion" as described in Kotelyanskii et al. 1998. Thus, thin film modification of water membranes have mainly found use in reverse osmosis, where a hydrostatic pressure is required to force the water through the membrane, and the obtained advantage lies in the improved separation of unwanted solutes in the water to be filtered. These conventional membranes for reverse osmosis have effectively 100-200 nm thick non-porous layers supported by a porous material. Water permeation in these membranes occurs as a diffusion process through the non-porous layer established via the appearance and disappearance of interstitial spaces. The HF module of the present invention may be further improved relative to the prior art reverse osmosis membranes by having aquaporin water channels incorporated in the thin film layer making it a thin film composite (TFC) layer. The incorporation of aquaporins have the added benefit of providing a selective water transport through its pores having a diameter of only 2.4 A at its narrowest passage (AqpZ pore, cf. Wang et al. 2005) where an efficient single file water transport takes place. The HF module of the invention combines the advantages of having a thin film separation layer together with aquaporin water channels thus providing improved separation as well as water flux through Angstrom sized pores making the HF
module suitable for both reverse osmosis, forward osmosis, assisted forward osmosis, nanofiltration etc.
DEFINITIONS
The term "hollow fiber membrane" and "HF membrane" as used herein refers to any type of capillary membrane which can be used for liquid filtration purposes.
The term "polyethersulfone" as used herein refers to a membrane material used in the fabrication of hollow fiber modules. An example is the membrane material UltraPESTM
marketed by MembranaTM GmbH. A cross section microscope photo of an UltraPesTM

fiber is shown in Fig. 2, cf. MembranaTM GMBH.
"Aquaporin" as used herein refers to selective water channel proteins, including AqpZ
and SoPIP2;1 prepared according to the methods described by Maria Karlsson et al.
(FEBS Letters 537 (2003) 68-72) or as described in Jensen et al. US
2012/0080377 Al.

11 "Asolectin" as used herein refers to a soybean lecithin fraction [IV-S}, which is a highly purified phospholipid product containing lecithin, cephalin, inositol phosphatides &
soybean oil (synonym: azolectin).
"Block copolymer" as used herein refers to membrane forming or vesicle forming di- and tri-block copolymers having both hydrophilic (A or C) and hydrophobic (B) blocks; the diblock copolymers being of the A-B or C-B type which are able to form bilayers and the triblock copolymers being of the A-B-A or A-B-C type that form monolayers by self assembly, where all of the membranes have the hydrophobic layer in the middle.
Examples of useful diblock copolymers and examples of useful triblock copolymers are the following:
Species Supplier Formula n(hydrophobic) n(hydrophilic) P7258 Polymer Source TM E0481DMS70 70 48 P5809 Polymer SourceTM E015B016 15 16 P8365 Polymer SourceTM E025DMS8 8 25 P7259 Polymer SourceTM E048DMS14 14 48 P7261 Polymer Source TM E01 14DM S ta 14 114 P369 1B Polymer SourceTM MOXA6DMS35MOXA6 35 12 P8061 Polymer SourceTM MOXAI5DMS67MOXA15 67 30 P9548 Polymer SourceTM MOXA15DMSII9MOXA15 119 30 where EO-block-DMS-block, such as E025DMS8, represents poly(dimethylsiloxane-block-ethylene oxide-block), E0-block-B0-block, such as E018B018, represents poly(butylene oxide-block-ethylene oxide-block), and MOXA-block-DMS-block-MOXA-block, such as MOXA6DMS MOXA _ _35 _ .6, represents poly(2-methyloxazoline-block-dimethylsiloxane-block-2-methyloxazoline).
The diblock and triblock copolymers can be used as single components or as mixtures in the creation of biomimetic membranes, such as vesicles or planar membranes, for incorporation of the aquaporins having amphiphilic properties due to their native transmembrane properties and functions.

12 "Liquid membrane" as used herein refers to membrane systems as disclosed in W02010/146365 (Aquaporin A/S) and WO 2012/080946 (Aquaporin A/S). Said liquid membrane is an integral component of the TFC HF membranes of the invention wherein it is immobilized or encapsulated.
The term "assisted forward osmosis" (or "pressure assisted forward osmosis") as used herein refers to the concept of applying a mechanical pressure to the feed side of the membrane to enhance the water flux through synergising the osmotic and hydraulic driving forces.
"Thin-film-composite" or (TFC) Hollow Fiber Membranes as used herein are prepared using an amine reactant, preferably an aromatic amine, such as a diamine or triamine, e.g.1,3-diaminobenzene (m-Phenylenediamine > 99%,e. g. as purchased from Sigma-AldrichTM) in an aqueous solution, and an acyl halide reactant, such as a di-or triacid chloride, preferably an aromatic acyl halide, e.g. benzene-1,3,5-tricarbonyl chloride (CAS
No. 84270-84-8, trimesoyl chloride (TMC), 98%, e.g. as purchased from Sigma-AldrichTM) dissolved in an organic solvent where said reactants combine in an interfacial polymerization reaction, cf. US 4,277,344 which describes in detail the formation of a composite membrane comprising a polyamide laminated to a porous membrane support, at the surface of the support membrane, e.g. a polyethersulfone membrane.
Benzene-1,3,5-tricarbonyl chloride is dissolved in a solvent, such as a C6 ¨ C12 hydrocarbon including hexane (>99.9%, Fisher ChemicalsTm), heptane, octane, nonane, decane etc.
(straight chain or branched hydrocarbons) or other low aromatic hydrocarbon solvent, e.g.
IsoparTM G Fluid which is produced from petroleum-based raw materials treated with hydrogen in the presence of a catalyst to produce a low odour fluid the major components of which include isoalkanes. IsoparTM G Fluid: Chemical Name: Hydrocarbons, C10-C12, isoalkanes, <2% aromatics; CAS No: 64742-48-9, chemical name: Naphtha (petroleum), hydrotreated heavy (from ExxonMobilTm Chemical). Alternatives to the reactant 1,3-diaminobenzene include diamines such as hexamethylenediamine etc., and alternatives to the reactant benzene-1,3,5-tricarbonyl chloride include a diacyl chloride, adipoyl chloride etc. as known in the art.
"Gas" as used herein means any gaseous fluid, such as inert gases, dinitrogen, atmospheric air, etc. that can be used for blowing off the solvent,

13 Proteoliposomes as used herein typically have a lipid to protein ratio (LPR
calculated on a mole basis) of between 25 to 500, such as about 100 to about 200.
Proteopolymersomes as used herein typically have a polymer to protein ratio (POPR
calculated on a mole basis) of between 25 to 500, such as about 50 to about 100 when using a triblock copolymer and a polymer to protein ratio of between 25 to 500, such as about 100 to about 200 when using a diblock copolymer.
In a preferred embodiment of the invention the hollow fiber bundles comprise a polyethersulfone (U1traPESTM) support material in the form of a hydrophilic capillary membrane material having the following characteristics (mean values are given):
Physical characteristics: Wall thickness 220 gm 15 gm; inner diameter 760gm gm; tensile strength? 410 cN; elongation at break? 40 %; explosion pressure?
12 bar;
implosion pressure? 7 bar; Minimal pore size of 6 ¨ 7 nm.
Membrane performance characteristics: Transmembrane flow (water, 25 C)? 0.65 m1/[min x cm' x bar]; molecular weight cut off MWCO (dextran, 90%, 0 bar) 65 kD 20 ka The characteristics given are representative of a preferred UltraPES material provided by MembranaTM GmbH, Oehder Stree 28, D ¨ 42289 Wuppertal, Germany.
In another preferred embodiment of the invention the hollow fibre bundles comprise the polyethersulfone MicroPES , such as the TF10 version also provided by MembranaTM
GmbH, support material, which differs from the UltraPES material in having a Transmembrane flow (water, 25 C)? 35 m1/[min x cm' x bar]; wall thickness of 100 gm 25 gm; an inner diameter of 300gm 40 gm; tensile strength of? 50 cN;
elongation at break? 30 %; maximum pore size of 0.5gm 0.1 gm.
EXPERIMENTAL EXAMPLES
Preparation of lmg/mL Asolectin proteoliposomes, and lipid to protein ratio (LPR) 200 using AqpZ Mw 27233 according to the following protocol:

14 1) Fill a 50 mL glass evaporation vial with 5 mL of a 2 mg/mL stock solution of asolectin (mW 786.11 g/mol, Sigma) in CHC13.
2) Evaporate the CHC13 using a rotation evaporator for at least 2h to complete dryness.
3) Add 0.8 mL of buffer solution (1.3% octylglucoside (OG) in PBS pH 7.4) to rehydrate the film obtained in the evaporation vial in step 2.
4) Shake the vial at maximum rpm on a platform shaker (HeidolphTM orbital platform shaker Unimax 2O1OTM or equivalent) until the lipid is dissolved.
5) Add 1.73 mg of AqpZ in a protein buffer containing Tris pH8, glucose and OG, 10 mg/mL, and rotate vial for 15 min at 200rpm, the AqpZ being prepared according to description herein.
6) Slowly add 9.03 ml PBS (pH 7.4 without OG), and shake vial for 15 min at 200rpm.
7) Freeze/thaw the combined solution/suspension on dry ice/40 C water bath for three times to eliminate possible multilamellar structures.
8) Add 250mg of hydrated BiobeadsTM (SM2 from BioRadTM) and rotate vial for lh at .. 200rpm at 4 C to adsorb detergent (OG).
9) Add further 250mg of hydrated BiobeadsTM and rotate vial for 2 to 3 days at 200rpm at 4 C.
10) The BiobeadsTM with adsorbed OG are then removed by pipetting off the suspension.
11) Extrude the obtained suspension for about 11 times through a 200nm polycarbonate filter using an extruder (such as an EmulsiF1exC5TM from AvestinTM, Canada) at least 1 time and up to about 22 times to obtain a uniform proteoliposome suspension (vesicles) suspension.
Protocol for 1mg/m1 proteo-polymersomes, protein to polymer ratio (POPR) 50 Polyoxazoline Based Triblock Copolymers, Poly(2-methyl oxazoline-b-dimethyl siloxane-b-2-methyl oxazoline, Moxa 12: DMS 35, Mw 3510) (P3691 purchased from Polymer SourceTM, Quebec, Canada), AqpZ Mw 27233 1) Fill a 50 ml glass evaporation vial with 5m1 of a 2 mg/ml stock solution of P3691 in CHC13.
2) Evaporate the CHC13 using a rotation evaporator for at least 2h to complete dryness.
3) Add 3.0 mL of buffer solution (1.3% 0.G.; 200mM Sucrose; 10mM Tris pH 8;
50mM
NaC1) to rehydrate the film obtained in the evaporation vial in step 2.
4) Shake the vial at 200 rpm on a platform shaker (HeidolphTM orbital platform shaker Unimax 2O1OTM or equivalent) for 3 hours to obtain dissolution of the copolymer.

5) Add 1.55mg 1_, of AqpZ in a protein buffer containing Tris, glucose and OG, and rotate vial over night at 200rpm and 4 C.
6) Add 6.88 ml buffer (10mM Tris pH 8; 50mM NaCl) slowly while mixing up and down with pipette.
7) Add 180mg hydrated BiobeadsTM and rotate for lh at 200rpm.
8) Add 210mg hydrated BiobeadsTM and rotate for lh at 200rpm.
9) Add 240mg hydrated BiobeadsTM and rotate O.N. at 200rpm 4 C.
10) Add 240mg hydrated BiobeadsTM and rotate O.N. at 200rpm 4 C.
11) The BiobeadsTM with adsorbed OG are then removed by pipetting off the suspension.
12) Extrude the suspension for about 21 times through a 200nm polycarbonate filter using an extruder, such as from at least 1 time and up to about 22 times to obtain a uniform proteopolymersome suspension (vesicles) suspension.
Example 1. Preparation of a hollow fiber module wherein the inside surface of the fibres has been functionalised with immobilised AqpZ vesicles Using a hollow fiber module having polyethersulfone membranes, such as a custom-made module, such as having 9 fibers corresponding to about 10 cm2 outside area and 5 cm2 inside area, or such as having a membrane area of up to 0.5 m2 which may correspond to several hundred fibers depending on module length (MembranaTm GmbH, Wuppertal, Germany), the module being prepared essentially as described by Sukitpaneenit et al.
2011, a thin film composite layer is prepared on the inside fiber surface through interfacial polymerization involving the following steps:
1) Obtaining 4 mL of AqpZ vesicles as prepared in the example above.
2) Dissolve 250 mg of 1,3-diaminobenzene in 6 mL of Mi11iQTM water to obtain a solution of 4.2% (w/w) concentration.
3) 75 mg ofbenzene-1,3,5-tricarbonyl chloride is dissolved in 50 mL of hexane to obtain a final concentration of 0.15% (w/v) 4) A 1,3-diaminobenzene/AqpZ vesicle mixture is prepared by dissolving/mixing 4 mL of the vesicles preparation from step 1 with 6 mL of the solution from step 2.
5) The mixture obtained in step 4 is constantly pumped through the module for 2 minutes using end inlet 1 (or inlet 2), cf. Fig. 1.
6) Excess 1,3-diaminobenzene is removed by a constant air purging of the lumen side of the fibers for 2 minutes using, e.g., inlet 1, cf. Fig. 1, preferably holding the module upside down.

7) A constant flow of the benzene-1,3,5-tricarbonyl chloride solution from step 3 is then injected into the module through inlet 1 for approximately 30s using a syringe pump, e.g.from TSE systemsTM, cf. to allow the interfacial polymerization reaction to take place.
8) Finally, the module is preferably rinsed with MilliQTM water, approximately 10 mL are used, by injection through side inlet 3 and 4.
After filling it with water the module is sealed with the white sealing caps (5), cf.
fig. 1, to prevent it from drying out (the sealing caps are part of the module and it is delivered with them).
Alternatively, steps 2 and 3 are as described below where all other steps are the same as shown above:
2) Dissolve 1,3-diaminobenzene in MilliQTM water to obtain a solution of 4.2%
(w/w) concentration.
3) benzene-1,3,5-tricarbonyl chloride is dissolved in a solvent such as hexane or isoalkane hydrocarbon solvent to obtain a final concentration of 0.15% (w/v).
Example 2. Preparation of a hollow fiber module wherein the inside surface of the fibres has been functionalised with immobilised AqpZ vesicles Using the same hollow fiber module as in Example 1 a thin film composite layer is prepared on the inside fiber surface through interfacial polymerization involving the following steps:
1) Obtaining 4 mL of AqpZ vesicles as prepared in the example above.
2) Dissolve 250 mg of 1,3-diaminobenzene in 6 mL of MilliQTM water to obtain a solution of 4.2% (w/w) concentration.
3) 75 mg of benzene-1,3,5-tricarbonyl chloride is dissolved in 50 mL of hexane to obtain a final concentration of 0.15% (w/v) 4) A 1,3-diaminobenzene/AqpZ vesicle mixture is prepared by dissolving/mixing 4 mL of the vesicles preparation from step 1 with 6 mL of the solution from step 2.
5) The mixture obtained in step 4 is constantly pumped through the module for 2 minutes using end inlet 1 (or inlet 2), cf. Fig. 1.
6) Excess 1,3-diaminobenzene is removed from the module by a constant stream of an organic fluid such as hexane for lmin through inlet 1 using a syringe pump.

7) A constant flow of the benzene-1,3,5-tricarbonyl chloride solution from step 3 is then injected into the module through inlet 1 for approximately 30s using a syringe pump, e.g.from TSE systemsTM, cf. to allow the interfacial polymerization reaction to take place.
8) Finally, the module is preferably rinsed with MilliQTM water, approximately 10 mL are used, by injection through side inlet 1 and 3.
After filling it with water the module is sealed with the white sealing caps (5), cf.
fig. 1, to prevent it from drying out (the sealing caps are part of the module and it is delivered with them).
Alternatively, steps 2 and 3 are as described below where all other steps are the same as shown above:
2) Dissolve 1,3-diaminobenzene in MilliQTM water to obtain a solution of 4.2%
(w/w) concentration.
3) benzene-1,3,5-tricarbonyl chloride is dissolved in a solvent such as hexane or isoalkane hydrocarbon solvent to obtain a final concentration of 0.15% (w/v).
Example 3. Preparation of a hollow fiber module wherein the inside surface of the fibres has been functionalised with immobilised AqpZ vesicles Using the same hollow fiber module as in Example 1 a thin film composite layer is .. prepared on the inside fiber surface through interfacial polymerization involving the following steps and using a syringe pump to push solutions through the module:
1) Obtaining 4 mL of AqpZ vesicles as prepared in the example above.
2) Dissolve 250 mg of 1,3-diaminobenzene in 6 mL of MilliQTM water to obtain a solution of 4.2% (w/w) concentration.
3) 75 mg of benzene-1,3,5-tricarbonyl chloride is dissolved in 50 mL of hexane to obtain a final concentration of 0.15% (w/v).
4) A 1,3-diaminobenzene/AqpZ vesicle mixture is prepared by dissolving/mixing 4 mL of the vesicles preparation from step 1 with 6 mL of the solution from step 2.
5) Add the solution from step 2 through the inside of the fibers while holding the module vertically with inlet down making sure that the air is let out); the solution can preferably be pumped using a flow rate of about 5 mL/min and continue pumping the solution through for 2 min, e. g. such as starting timing immediately after the solution could be seen in upper end of module.

6) Disconnect the module from the syringe pump and turn it around to have excess solution flow out into collection glass.
7) Connect the module upside down to air and slowly start air flow until 10 L/min is reached; let air flow for 2 min.
8) Connect the module to a benzene-1,3,5-tricarbonyl chloride solution syringe, hold the module in vertical position and start benzene-1,3,5-tricarbonyl chloride/hexane flow. e.g.
while keeping a flow rate of about 15 mL/min.
9) Disconnect module from hexane syringe and turn upside down to get last hexane out;
connect to air and purge at about 10 L/min for 5-10 s.
10) Fill module with MilliQTM by sucking it in from a glass container.
Alternatively, steps 2 and 3 are as described below where all other steps are the same as shown above:
2) Dissolve 1,3-diaminobenzene in Mi1liQTM water to obtain a solution of 4.2%
(w/w) concentration.
3) Benzene-1,3,5-tricarbonyl chloride is dissolved in a solvent such as hexane or isoalkane hydrocarbon solvent to obtain a final concentration of 0.15% (w/v).
Alternatively, in the pHF22 protocol we use a syringe pump to push solutions through the module, such as a MicroPES-TF10 HF module, then after obtaining 4 mL of AqpZ
vesicles as prepared in the example above, follow the steps below:
1) Dissolve MPD in MilliQTM water and add the AqpZ vesicles to get a 2.5%(W/W) concentration of MPD in water/vesicle solution 2) Dissolve TMC in an organic solvent, such as hexane or an isoalkane hydrocarbon solvent, to a final concentration of 0,15% WN
.. 3) push MPD solution through the inside of the fibers while holding it vertically with inlet down (while filling the module repeatedly shake it to get the air out); 5 mL/min flow rate 4) continue pushing MPD solution through for 1 min (time starts after MPD
solution could be seen in upper end of module) and then let it soak with MPD solution inside for 1 min 5) disconnect module from syringe and turn it around to have excess MPD flow out into collection glass 6) connect the module upside down (meaning end with number on top) to air and slowly start air flow; dry with controlled air stream for 1 to 2 min and turn module in between 7) connect to TMC solution, hold vertical (numbered end on bottom) and start TMC
solution flow (flowrate: 10 mL/min) 8) let solution run through the fibers for 45 s (after the module is filled, it can be tilted back to horizontal position) 9) disconnect module from syringe and turn upside down to get last TMC
solution out;
connect to air and purge at 10 L/min for 5-10 s 10) fill module with MilliQTM by sucking it in from a glass Following the various methods outlined above a TFC-aquaporin modified hollow fiber module is obtained where the inner surface of the fibers have acquired a novel microstructure as shown in Fig. 3, where (6) refers to the TFC layer ¨200nm and (7) refers to the support membrane of 220 m, (6) and (7) together represents a cross section of the hollow fiber wall; (8) schematically represents the bilayer membrane in the case of lipids and diblock copolymers as amphiphilic membrane forming materials having hydrophilic head groups and hydrophobic tail groups. Feature (8) would show a monolayer having a hydrophobic middle portion and hydrophilic end portions or would show a type of bilayer where the triblock copolymers assume a horseshoe conformation or mixtures of both when triblock copolymers are used for the amphiphilic membrane material. (9) Represents the aquaporin protein where its tetrameric form is shown.
However, the protein may also be present as a monomer or as a dimer, both of which are also water channels.
Filtration results using forward osmosis Tables 1 and 2 below show filtration results in the form of measured water flux, Jw, and measured reverse salt flux, Js, for HF modules prepared according to the invention using a feed solution of 10 [tM calcein (CAS No. 1461-15-0, Mw 622.55) and a draw solution of 2 M NaCl both in deionized water (MilliQTm water) and where the TFC-aquaporin layer is formed on the inside of hollow fibers (lumen) and the draw solution is applied to the outside or inside of the fibers. Co-current or counter-current flow was used. Referring to Fig. 1: Typically, feed solution was pumped through inlet (1) and out through (2), and draw solution was pumped through inlet (3) and out through (4). In five experiments a counter-current flow was used, cf. Tables 1 and 2 and description below. The water channel protein AqpZ was used in all experiments, and both a lipid (asolectin) and various block copolymers (P3691, P8061) were vesicle membrane materials.

Each experiment is characterised by the water flux (Jw), the reverse salt flux (Js) and the calcein rejection. The water flux describes how much water is transported over a certain amount of membrane area in a given time (usually L/m2h) and is measured by monitoring the weight loss in the feed solution. The reverse salt flux characterises the amount of draw solute that diffuses back into the feed solution. It is an indicator on how salt-tight a membrane is. The Js is determined by measuring the conductivity changes in the feed solution. The calcein rejection is used to determine the forward rejection properties of a membrane. Here, a fluorescent marker, that is easily detectable by a desktop fluorometer (e.g. QubitTM fluorometer, InvitrogenTm), is added to the feed solution. By measuring its concentration in draw we can determine how much of the marker is rejected.
Water flux is calculated as following:
Jw¨Vt/(A*0 ; [L/m2h], where Vt is the transported Volume, A the active membrane area and t the time in hours.
To determine the reverse salt flux we have to measure the conductivity in the feed solution. With the help of a calibration curve we can then relate the conductivity to a salt concentration and thus calculate the reverse salt flux as follows:
Js= (cf,end*Vf,end-cf,start*Vf,start)/(A*t) ; [g/m2h], where Vf marks the start and end volume and cf the start and end concentration of salt in feed.
The calcein rejection is approximated by:
Rca=1-((Vd,end*cca,d,end-Vd,start*cca,d,start)/(Vt*cca,f,start)) Where Vd is the start and end draw volume and cca,d the calcein reads in the draw solution. The starting concentration of calcein in feed (cca,f) is taken as a constant. This is an approximation because a membrane that rejects calcein will up-concentrate the calcein concentration in feed. However, this can be tolerated because by leaving the start concentration constant in the calculation we underestimate the total rejection, which is acceptable.
Table 1 HF module Flow speed Jw J, Js/ Jw Calcein Run time No. ImL/min] Water Reverse [g/L] Rejection Flux salt flux icyd [L/m2h] [g/m2h]

(Thin film feed=10 layer only) draw=50; a 14.07 1.67 0.12 99.97 < 3 h _ 12-0636 feed=10 Asolectin d draw=50; a 14.66 5.30 0.36 99.98 360 min 12-0636 feed=10 Asolectin d draw=50, a 21.88 4.77 0.22 99.97 360 min feed=50 12-0630 draw=10;
P3691 d ab 17.06 5.17 0.30 99.89 380 min Notes: a counter-current flow; ab draw on inside & counter-current flow; d according to Ex. 1. HF module No. 12-0644 has been modified with thin film layer only without vesicles and/or protein and thus represents prior art as disclosed by Sukitpaneenit &
Chung 2012.
Table 1 clearly shows that when using the HF module of the invention it is possible to obtain a greater water flux, Jw, of up to 3 L/m2h above the flux obtainable for a thin film modified HF module having 9 fibers while still maintaining a good Js/Jw ratio of as low as about 0.22. This will be an advantage for filtration purposes, where a high water flux is desirable while less efficient salt retention can be tolerated, e.g. in the treatment of wastewater. The results according to the invention shown in Table 1 are all obtained using .. counter-current flow, which could prove an advantage for high water flux.

Table 2 HF module Flow speed Jw Js Js/ Jw Calcein Run No. [mL/min] Water Reverse [g/L] Rejection time Flux salt flux 10/01 [L/m2h] [g/m2h]
12-0644 (thin film layer feed=10 only) draw=50; a 14.07 1.67 0.12 99.97 < 3h _ 12-1470 feed=50 P8061 dd draw=10; c 8.72 1.17 0.13 99.01 900 min 12-1474 feed=50 P8061 dd draw=10; c 7.71 1.44 0.19 99.27 900 min Notes: a counter-current flow; c draw on inside; dd prepared according to Ex.
3. HF
module No. 12-0644 has been modified with thin film layer only without vesicles and/or protein and thus represents prior art as disclosed by Sukitpaneenit & Chung 2012.
Table 2 clearly shows that using the HF module of the invention it is possible to obtain an increase in reverse salt retention, Js, of about 0.5 g/m2h corresponding to a 25 % increase compared to the reverse salt retention obtainable for a thin film modified HF
module having 9 fibers while still maintaining a good water flux of about 8 to 9 L/m2h. These results are obtained using co-current flow with the draw solution on the inside of the HF
module fibers. All of the above calcein rejection values are better than 99 %
showing that the HF modules used are extremely tight (no membrane leakage). The HF module of the invention can be used with both co¨current flow and counter-current flow, and as seen in the results above it can be preferred to have counter-current flow. In that case the differences in osmotic pressure are more evenly distributed over the whole length of the fiber. When both feed and draw solutions enter at the same side (co-current flow), then water is immediately pulled out of the feed stream into the draw stream, and along the fiber the difference in osmotic pressure will decrease rapidly (feed is concentrated and draw diluted). When the HF module is now operated with counter-current flow, then we have a cleaner feed meeting a diluted draw in one end and a high osmotic draw meeting a weakly osmotic feed (salt contaminated) at the other end. So the osmotic pressure difference between both liquids is closer to being the same along the length of the fiber.

This might favour counter-current flow. However, what speaks in favour of co-current flow is that the pressure which is generated by pumping inside is met with an equal pressure that is generated by pumping on the outside of the fiber.
Table 3 showing results from 4 runs using the alternative method described above (PHF22) J J / J Calcein Protocol Water flux Reverse salt flux [g/L] Rejection [L/m2h1 [g/m2hi [%1 4.04 0.75 0.19 99.98 pHF22 MicroPES-TF10 fibers 4.52 1.02 0.23 99.88 Experiment run for 3.5 4.61 0.86 0.19 99.88 hours 8.1 1.3 0.16 99.94 Average 5.32 0.98 0.19 99.92 Table 4 showing results from 4 runs using the alternative method described above (PHF22) Calcein Protocol Water flux Reverse salt flux [g/L]
Rejection [L/m2h] [g/m2h] 10/01 12.2 2.06 0.17 99.95 pHF22 IRES-TF10 fibers 13.62 2.41 0.18 99.97 Experiment run for 3.5 16.48 2.5 0.15 99.98 hours 17.34 2.55 0.15 99.77 Average 14.91 2.38 0.16 99.92 Table 5 showing results from 4 runs using the alternative method described above (PHF22) Protocol J, Water Flux Js Reverse Js/ J Calcein [L/m2h] salt flux [g/L]
Rejection 1g/m2h] 1 /0]
pHF22 (slower pumping speed 16.12 3.6 0.22 99.98 for TMC solution (5 mL/min instead of 10 mL/min)) 16.7 3.44 0.21 99.96 Flow speed [mL/min]: feed=10 9.56 1.69 0.18 99.69 draw=50 Experiment run for 200 mins 9.58 2.15 0.22 99.99 Average 12.99 2.72 0.21 99.91 The inside coating of HF MicroPES TF10 5 cm2 active membrane area hollow fibre modules resulted in high reproducibility; All experiments in Tables 3 and 4 have comparable Js/Jw ratios with varying Jw and Js which can be due to differences/tolerances in experiments, differences in measuring accuracy, and possibly due to the fact that the coating was a hand-made process. Thus, the two different experiments resulted in HF modules having comparable Js/Jw but varying average ranges of Jw and Js, cf. Table 3 showing experimental results of moderate Jw and low Js, and Table 4 showing experimental results of high Jw and moderate Js.
Example 4. Preparation of a hollow fiber module wherein the outside surface of the fibres has been functionalised with immobilised AqpZ vesicles Using a hollow fiber module having polyethersulfone membranes, such as a custom-made module, such as having 9 fibers corresponding to about 10 cm2, or such as having a membrane area of up to 0.5 m2 which may correspond to several hundred fibers depending on module length (MembranaTm GmbH, Wuppertal, Germany), a thin film composite layer being prepared on the outside fiber surface through interfacial polymerization involving the following steps of protocol PHF21:
1) Obtain 4 mL of AqpZ vesicles as prepared in the example above.
2) Dissolve 1,3-diaminobenzene in MilliQTM water to obtain a solution of 4.2%
(w/w) concentration.

3) benzene-1,3,5-tricarbonyl chloride is dissolved in an organic solvent, such as hexane or an isoalkane hydrocarbon solvent,to obtain a final concentration of 0.15%
(w/v) 4) A 1,3-diaminobenzene/AqpZ vesicle mixture is prepared by dissolving/mixing 4 mL of the vesicles preparation from step 1 with 6 mL of the solution from step 2.
.. 5) fill solution from step 4. into the module on the outside of the fibers (side inlets of the module); flowrate: 5mL/min 6) stop flow after 1 min and leave fibers soaking for 1 min 7) empty the module and purge with air to get leftover MPD solution out 8) use air flow to remove surface water from the fibers (air flow rate 25 L/min) 9) pump solution from step 3. into the module using a flow rate of 15 mL/min 10) after module is filled continue pumping for 30s 11) disconnect module from syringe and turn upside down to get last solution out;
connect to air and purge at 10 L/min for 5-10 s 12) fill module with Mi1liQTM by sucking it in from a glass Table 5 showing results from 4 runs using the method described above (PHF21) Protocol J, J J / J Calcein PHF21 according to Ex. 4 Water flux Reverse salt flux Ig/L] Rejection [L/m2h] [g/m211] i%1 UltraPES fibers Outside coating (20 L/min) 6.36 2.09 0.33 99.66 Experiment run for 900 min UltraPES fibers 6.63 1.1 0.17 99.96 Outside coating (25 L/min) 8.57 3.95 0.46 99.57 Experiment run for 900 min 8.14 3.97 0.49 99.72 Average 7.43 2.78 0.36 99.73 The outside coating of HF UltraPES TF10 5 cm2 active membrane area hollow fibre modules resulted in high reproducibility: The experiments in Table 5 show sets of 2 (roughly 2 x 8 [L/m2h] and 2 x 6 [L/m2h]) that have comparable Jw and Js values.

Example 5. Inside coating for creation of TFC layer on fibres in MicroPES TF10 HF
module Protocol HF24 for the inside coating of 100cm2 modules using a syringe pump to suck solutions through the module:
Use a syringe pump to pump solutions through the module 1) pre-soak modules with MilliQTM at least over night 2) Obtain 16 mL of AqpZ vesicles as prepared in the example above.
3) Dissolve 1,3-diaminobenzene in MilliQTM water to obtain a solution of 4.2%
(w/w) concentration.
4) 75 mg of benzene-1,3,5-tricarbonyl chloride are dissolved in 50 mL of hexane to obtain a final concentration of 0.15% (w/v) 5) A 1,3-diaminobenzene/AqpZ vesicle mixture is prepared by dissolving/mixing 16 mL
of the vesicles preparation from step 1 with 24 mL of the solution from step 3.
6) pump solution from step 5. through the module for as long as it takes until no more bubbles come out (tap it continuously to shake bubbles out) at 10 mL/min; keep the module vertically 7) Let solution soak in fibers for 1 min 8) disconnect module from syringe and let excess solution flow out into collection glass 9) connect the module upside down to air and slowly start air flow until 20 L/min are reached 10) dry with controlled air stream for 3 to 5 min and turn module in between 11) push solution from step 4. through the fibers at a constant flow (flowrate: 15 mL/min) for 45s (starting from when TMC enters the fibers) 12) empty the module by disconnecting the tube 13) blow air through the fibers for 5 to 10 s to eject leftover solution 14) fill fibers and module with MilliQTM

, Table 6 showing results from 2 runs using the method described above (PHF24) J, J, / J Calcein Rejection Water flux Reverse salt [g/Lj ['A]
Protocol [L/m211] flux tonzhi pHF24;
10.83 1.21 0.11 99.73 MicroPES-TF10 fibers; 2M NaC1 as draw 14.21 1.65 0.12 99.88 Experiments run for 200 min Average 12.52 1.43 0.11 99.81 Table 7 showing results from 3 runs using the method described above (PHF24) J J / J Calcein Water flux Reverse salt [g/L] Rejection Protocol [L/m2111 flux icyoi [wm2h1 pHF24;MicroPES- 8.26 1.33 0.16 99.97 TF10 fibers; 1M
NaCl as draw 7.66 1.72 0.22 99.96 Experiments run for 200 min 6.01 1 0.17 99.74 Average 7.31 1.35 0.18 99.89 The inside coating of HF MicroPES TF10 100 cm2 active membrane area hollow fibre modules resulted in high reproducibility, cf. Tables 6 and 7showing experimental results with 2M NaCl as draw solution and Table 6 showing experimental results with 1M
NaC1 draw solution where a reduction in water flux is notable in contrast to a minor reduction in reverse salt flux Example 6. Hollow fibers outside continuous coating tests Purpose of this experiment is to establish a method of continuous coating on the outside of hollow fiber membranes via an automatized production process.
Materials The machine and fibers are provided by MembranaTM and the coating chemicals and AqpZ vesicles are the same as used in the previous examples. The different hollow fiber membranes tested are MicroPES TF10 and the DuraPES 0.7 (MembranaTm GmbH, Wupperthal, Germany). Thus, the final coating holds an immobilized liquid membrane component containing aquaporin proteins.
Methods Figure 4 illustrates in the form of a sketch the automatized continuous outside coating of hollow fibers (shown as a narrow line). In the figure: (10) is a coil of uncoated hollow fibers, preferably of a porous polyethersulfone (or polysulfone and the like) material.
From here they are transported into the aquaporin liquid membrane/MPD bath (11) where the aquaporin vesicles will soak into the fiber and attach to its surface.
Excess MPD/aquaporin solution is removed during a drying step (12) where an air knife can be used to enhance excess solution removal. From here the fiber passes into the TMC/
isoalkane hydrocarbon solvent bath where the interfacial polymerization takes place (13).
Thus the aquaporin vesicles (proteopolymersomes of the liquid membrane) are encapsulated due to the TFC layer formed on the fiber. A drying step (14) ensures that all of the organic solution is evaporated before dipping the fiber into a water bath to remove left-over chemicals (15). Now the fiber is wound up on a new coil (16). The coated hollow fibers are then cut to the appropriate length and built into modules suitable for FO
water extraction and separation purposes.
Example 7. Hollow fiber module retention and up-concentration assay of two small peptides.
In this example we used HF modules having 5cm2 active area prepared according to Example 3 above.
Peptide A: GGG SGA GKT MW 0.692 kDa 100 mL of GGG SGA GKT peptide (MW 0.692 kDa) in TES buffer (feed solution) was filtered through a forward osmosis HF module until desired up-concentration (approximately 20x) using 1M NaCl as draw solution. The weight of the up-concentrated sample was measured to determine the volume reduction from the initial start sample. 10 pl of the up-concentrated sample was mixed with 90 p.1 of 10x TES buffer to eliminate the up-concentration factor of the buffer when determining the end concentration of the up-concentrated peptide sample. The total sample of 100 p.1 was then mixed with 100 pl of LavaPepTM quantification kit, incubated for 1 h in room temperature and then the fluorescence counts were read in a QuBitTM fluorometer (InvitrogenTm). The fluorescence counts were then compared to a standard curve where the actual concentration of the peptide sample was determined. From the same initial up-concentrated peptide sample, three samples (n=3) were prepared and measured in the QubitTM and the mean fluorescent count number was used to determine the concentration from the standard curves.
The sample volume was concentrated about 20 times, and the peptide A was up-concentrated about 18 to 19 times.
Peptide B: AGKT MW 0.375 kDa (experimental conditions closely corresponding to those described above for peptide A).
100 mL of AGKT peptide (MW 0.375 kDa) in TES buffer was run until desired up-concentration (approximately 20x) with the FO hollow fiber module. The mass of the up-concentrated sample was measured to determine the volume reduction from the initial start sample. The up-concentrated sample was then diluted 4 times with TES
buffer to generate a 5 times up-concentrated peptide sample prior to further sample processing.
This is done to avoid the quenching of the fluorescent signal for the smaller peptide as it has been observed in previous assays. 10 pl of the 5x up-concentrated sample was mixed with 90 1 of 10x TES buffer to eliminate the up-concentration factor of the buffer when determining the end concentration of the up-concentrated peptide sample. The total sample of 100 ul was then mixed with 100 pi of LavaPepTM quantification kit, incubated for 1 h in room temperature and then the fluorescence counts were read in a QuBitTM
fluorometer (InvitrogenTm). The fluorescence counts were then compared to a standard curve where the actual concentration of the peptide sample was determined.
From the same initial up-concentrated peptide sample, three samples (n=3) were prepared and measured in the QubitTM and the mean fluorescent count number was used to determine the concentration from the standard curves. The sample volume was concentrated about 21 times, and the peptide B was up-concentrated about 24 times.
In both cases the up-concentration factor of the sample peptides A and B
corresponds to the reduction factor of the volume, thus leading to the conclusion that the Hollow Fiber modules having an active aquaporin-TFC layer on the inside can be used to up-concentrate biomolecules at least down to sizes of 0.375 kDa.
Materials and equipment:
Peptides: GGG SGA GKT purchased from CASLOTM
TES: N-(Tris(hydroxymethyl)methyl)-2-aminomethanesulfonic acid), Sigma AldrichTM, Cas 7365-44-8 LavaPepTM peptide quantification kit Part A (LP-022010) Part B (LP-022010) QubitTM fluorometer, InvitrogenTM
Catalog number: Q32857 Serial number: 45257-233 Measurement setting: Quant-iTTm ssDNA
TES buffer, 100mL
m (TES) = 229.8 mg m(EDTA) = 37.2mg Adjust pH with 1M NaOH to 8 and fill up with mQ water. Filter through a vacuum filter.
For 10x TES buffer the TES and EDTA amounts are multiplicated by 10.
LavaPepTM quantification kit.
Part A, Part B and mQ water are mixed together following the ratio (1:1:8).
LavapepTM working solution is mixed with the up-concentrated peptide sample following the ratio (1:1).
8. Hollow fiber module retention of creatinine In this example we will, i.a., use HF modules having 5cm2 active area prepared according to Example 3 above. The purpose is to determine the retention rate of creatinine (MW
113.12 g moll), which occurs naturally in blood and urine. If the filtration in the kidney is deficient, creatinine blood levels will rise. The creatinine level in blood and urine is commonly used to calculate the creatinine clearance (CrC1), which correlates with the glomerular filtration rate (GFR) which is clinically important as a measurement of renal function.
Creatinine assay Creatinine in a sample is detected with the Creatinine Assay kit from abcamTM
(ab65340).
In the assay creatinine is converted to creatine by creatinase, creatine is converted to sarcosine which reacts with a probe to generate red color (lambda max = 570 nm) and fluorescence (Ex/Em=538/587 nm).
The instructions of the kit are followed without alterations. Creatininase, creatinase and creatine enzyme mix are reconstituted with 220 [A of Assay Buffer each and aliquoted prior to use to avoid freeze and thaw cycles. Creatinine standard is reconstituted with 100 I of deionized H20 to generate 100mM Creatinine Standard. For the colorimetric assay .. the creatinine standard is diluted a hundred times in Assay Buffer to generate a working stock solution of 1 nmol4t1. A dilution series is prepared where 0, 2, 4, 6, 8 and 10 1 of the working solution is mixed in assay buffer to a final volume of 50 1.
For each sample a reaction mix is prepared with the following volumes.
Assay Buffer: 42 1 Creatinase 2 pl Creatininase: 2 1 Enzyme mix: 2 1 Probe: 2 1 The background reaction mix contains the same reagents except for Creatininase. The amount of Assay Buffer is 44 pi instead. The standard samples (50 1) are mixed with the reaction mix (50 I), incubated at 37 C for one hour. O.D. is measured in micro cuvettes at 570 nm and the background is subtracted from all the samples. O.D. is then plotted against the concentration to generate a standard curve.
For creatinine samples in hollow fibers the same procedure will be done where the up-concentrated sample will be diluted 100 times in Assay Buffer and 50 I of the resulting sample will be mixed with 50 pi of the reaction mix. The measured O.D. value will be measured and the concentration of the sample will be determined from the standard curves.

References Panu Sukitpaneenit and Tai-Shung Chung, Environmental Science & Technology, 2012, 46, 7358-7365 Niwa T. Nagoya J Med Sci. 2010 Feb;72(1-2):1-11.
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Claims (16)

Claims:

1. A hollow fiber (HF) module comprising hollow membrane fibers modified with a thin film composite (TFC) layer comprising aquaporin water channels wherein the TFC
layer is a polyamide layer provided on the inside of the fibers, and wherein said aquaporin water channels are incorporated in liposomes or polymersomes.

2. The HF module according to claim 1, wherein said liposomes are prepared from lipids compris ing diphytanoyI phosphatidylcholine (DPhPC), 1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC), mixed soy bean lipids, asolectin or E. coli mixed lipids.

3. The HF module according to claim 1, wherein said polymersomes comprise triblock copolymers of the hydrophile-hydrophobe-hydrophile (A-B-A or A-B-C) type, diblock copolymers of the hydrophile-hydrophobe type (A-B), or a combination thereof.

4. The HF module according to any one of claims 1 to 3, wherein said aquaporin water channels are AqpZ channels or SoPIP2;1 water channels.

5. The HF module according to any one of claims 1 to 4, wherein said TFC
layer is formed through interfacial polymerization of an aqueous solution of a di- or triamine with a solution of di- or triacyl halide in an organic solvent, and wherein the liposomes or the polymersomes are incorporated in said aqueous solution.

6. The HF module according to any one of claims 1 to 5, wherein the hollow fibers comprise a polyethersulfone.

7. The HF module according to any one of claims 1 to 6, wherein the active surface area of the fibers is from about 0.1 cm2 to about 0.5 m2.

8. A hollow fiber (HF) membrane comprising hollow membrane fibers modified with a thin film composite (TFC) layer comprising aquaporin water channels, wherein the TFC layer is a polyamide layer provided on the inside of the fibers, and wherein said aquaporin water channels are incorporated in liposomes or polymersomes.

9. The HF membrane according to claim 8, wherein said liposomes are prepared from lipids comprising diphytanoyl phosphatidylcholine (DPhPC), 1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC), mixed soy bean lipids, asolectin or E. coli mixed lipids.

10. The HF membrane according to claim 8, wherein said polymersomes comprise triblock copolymers of the hydrophile-hydrophobe-hydrophile (A-B-A or A-B-C) type, diblock copolymers of the hydrophile-hydrophobe type (A-B) or a combination thereof.

11. The HF membrane according to any one of claims 8 to 10, wherein said aquaporin water channels are AqpZ channels or SoPIP2;1 water channels.

12. The HF membrane according to any one of claims 8 to 11, wherein said TFC layer is formed through interfacial polymerization of an aqueous solution of a di- or triamine with a solution of an di- or triacyl halide in an organic solvent, and wherein the liposomes or the polymersomes are incorporated in said aqueous solution.

13. A method of preparing a HF module, comprising the steps of:
a) obtaining an aquaporin vesicles suspension of proteoliposomes or proteopolymersomes having from about 25 to about 500 lipid to protein ratio (LPR)/polymer to protein ratio (POPR) of protein calculated on a mole basis, b) preparing an aqueous solution of a di- or triamine, c) dissolving a di- or triacyl halide in an apolar organic solvent, d) preparing a mixture of amine and aquaporin vesicle by dissolving/mixing the vesicles suspension from step a) with the solution from step b), e) pumping the mixture from step d) through the inside of the hollow fibers in a hollow fiber module using its end inlet, f) removing excess aqueous solution by a gas purging of the inside of the fibers using the end inlet, g) pumping the acyl halide solution from step c) into the module through the inside of the hollow fibers using the end inlet to allow an interfacial polymerization reaction to take place, and h) rinsing the module with an aqueous solvent by pumping through the end inlet.

14. The method according to claim 13, wherein the aqueous solution in b) is 1,3-diaminobenzene of about 1% to about 5% (w/w) concentration, wherein the di- or triacyl halide in c) is benzene-1,3,5-tricarbonyl chloride and the apolar organic solvent is selected from the group consisting of hexane, heptane, octane and mixtures thereof to obtain a concentration of about 0.05% to about 1% (w/v), and wherein the mixture of amine and aquaporin vesicle of d) is an 1,3-diaminobenzene/aquaporin vesicle mixture prepared by dissolving/mixing the vesicles suspension from step a) with the solution from step b).

15. The method according to claim 13 or 14, wherein said HF module comprises polyethersulfone fibers.

16. The method of claim 13 or 14, wherein in step f) the fibers are gas purged to dryness.