CA2891153C - Evenement dp-073496-4 de brassica gat et compositions et procedes pour l'identifier et/ou le detecter - Google Patents

Evenement dp-073496-4 de brassica gat et compositions et procedes pour l'identifier et/ou le detecter Download PDF

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Publication number
CA2891153C
CA2891153C CA2891153A CA2891153A CA2891153C CA 2891153 C CA2891153 C CA 2891153C CA 2891153 A CA2891153 A CA 2891153A CA 2891153 A CA2891153 A CA 2891153A CA 2891153 C CA2891153 C CA 2891153C
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CA
Canada
Prior art keywords
herbicide
plant
event
dna
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2891153A
Other languages
English (en)
Other versions
CA2891153A1 (fr
Inventor
David George Charne
Wenpin Chen
Chadwick Bruce Koscielny
Jayantilal Devabhai Patel
Ferdinand Gerard Thoonen
Lomas Tulsieram
Yongping Zhang
Zhongsen Li
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pioneer Hi Bred International Inc
Corteva Agriscience LLC
Original Assignee
Pioneer Hi Bred International Inc
EI Du Pont de Nemours and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi Bred International Inc, EI Du Pont de Nemours and Co filed Critical Pioneer Hi Bred International Inc
Priority to CA2891153A priority Critical patent/CA2891153C/fr
Priority to CA2995972A priority patent/CA2995972A1/fr
Priority claimed from CA2810180A external-priority patent/CA2810180C/fr
Publication of CA2891153A1 publication Critical patent/CA2891153A1/fr
Application granted granted Critical
Publication of CA2891153C publication Critical patent/CA2891153C/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
    • A01N57/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds
    • A01N57/20—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds containing acyclic or cycloaliphatic radicals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8277—Phosphinotricin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8278—Sulfonylurea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae

Abstract

Des compositions et des méthodes relatives aux plants de Brassica transgéniques tolérant le glyphosate sont présentées. En particulier, la présente invention fournit des plants de Brassica ayant un événement DP-073496-4 qui donne la tolérance au glyphosate. Le plant de Brassica présentant lévénement DP-073496-4 à lemplacement chromosomique indiqué comprend des jonctions génome/transgène dans la séquence SED ID NO: 2 ou les jonctions génome/transgène définies dans la SEQ ID NO: 12 ou 13. La caractérisation du site dinsertion génomique de lévénement permet une efficacité de reproduction améliorée et lutilisation de marqueurs moléculaires pour suivre linsertion du transgène dans les populations de reproduction et leur progéniture. Diverses méthodes et compositions destinées à lidentification, la détection et lutilisation de lévénement sont présentées.
CA2891153A 2010-11-24 2010-11-24 Evenement dp-073496-4 de brassica gat et compositions et procedes pour l'identifier et/ou le detecter Active CA2891153C (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CA2891153A CA2891153C (fr) 2010-11-24 2010-11-24 Evenement dp-073496-4 de brassica gat et compositions et procedes pour l'identifier et/ou le detecter
CA2995972A CA2995972A1 (fr) 2010-11-24 2010-11-24 Evenement dp-073496-4 de brassica gat et compositions et procedes pour l'identifier et/ou le detecter

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CA2891153A CA2891153C (fr) 2010-11-24 2010-11-24 Evenement dp-073496-4 de brassica gat et compositions et procedes pour l'identifier et/ou le detecter
CA2810180A CA2810180C (fr) 2010-11-24 2010-11-24 Evenement dp-073496-4 de brassica gat et compositions et procedes pour l'identifier et/ou le detecter

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CA2810180A Division CA2810180C (fr) 2010-11-24 2010-11-24 Evenement dp-073496-4 de brassica gat et compositions et procedes pour l'identifier et/ou le detecter

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA2995972A Division CA2995972A1 (fr) 2010-11-24 2010-11-24 Evenement dp-073496-4 de brassica gat et compositions et procedes pour l'identifier et/ou le detecter

Publications (2)

Publication Number Publication Date
CA2891153A1 CA2891153A1 (fr) 2012-05-31
CA2891153C true CA2891153C (fr) 2018-04-03

Family

ID=53199751

Family Applications (4)

Application Number Title Priority Date Filing Date
CA3159670A Pending CA3159670A1 (fr) 2010-11-24 2010-11-24 Evenement dp-073496-4 de brassica gat et compositions et procedes pour l'identifier et/ou le detecter
CA2891153A Active CA2891153C (fr) 2010-11-24 2010-11-24 Evenement dp-073496-4 de brassica gat et compositions et procedes pour l'identifier et/ou le detecter
CA3069427A Abandoned CA3069427A1 (fr) 2010-11-24 2010-11-24 Evenement dp-073496-4 de brassica gat et compositions et procedes pour l'identifier et/ou le detecter
CA2995972A Abandoned CA2995972A1 (fr) 2010-11-24 2010-11-24 Evenement dp-073496-4 de brassica gat et compositions et procedes pour l'identifier et/ou le detecter

Family Applications Before (1)

Application Number Title Priority Date Filing Date
CA3159670A Pending CA3159670A1 (fr) 2010-11-24 2010-11-24 Evenement dp-073496-4 de brassica gat et compositions et procedes pour l'identifier et/ou le detecter

Family Applications After (2)

Application Number Title Priority Date Filing Date
CA3069427A Abandoned CA3069427A1 (fr) 2010-11-24 2010-11-24 Evenement dp-073496-4 de brassica gat et compositions et procedes pour l'identifier et/ou le detecter
CA2995972A Abandoned CA2995972A1 (fr) 2010-11-24 2010-11-24 Evenement dp-073496-4 de brassica gat et compositions et procedes pour l'identifier et/ou le detecter

Country Status (1)

Country Link
CA (4) CA3159670A1 (fr)

Also Published As

Publication number Publication date
CA3069427A1 (fr) 2012-05-31
CA2891153A1 (fr) 2012-05-31
CA2995972A1 (fr) 2012-05-31
CA3159670A1 (fr) 2012-05-31

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Effective date: 20151009