CA2787759C - Process for preparing an a2a-adenosine receptor agonist and its polymorphs - Google Patents
Process for preparing an a2a-adenosine receptor agonist and its polymorphs Download PDFInfo
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- CA2787759C CA2787759C CA2787759A CA2787759A CA2787759C CA 2787759 C CA2787759 C CA 2787759C CA 2787759 A CA2787759 A CA 2787759A CA 2787759 A CA2787759 A CA 2787759A CA 2787759 C CA2787759 C CA 2787759C
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- oxolan
- dihydroxy
- hydroxymethyl
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- 101150051188 Adora2a gene Proteins 0.000 title abstract description 6
- 229940122614 Adenosine receptor agonist Drugs 0.000 title abstract description 5
- 239000003379 purinergic P1 receptor agonist Substances 0.000 title abstract description 5
- 238000004519 manufacturing process Methods 0.000 title 1
- 150000001875 compounds Chemical class 0.000 claims abstract description 44
- 238000000034 method Methods 0.000 claims abstract description 19
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 46
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 29
- BAVYZALUXZFZLV-UHFFFAOYSA-N Methylamine Chemical compound NC BAVYZALUXZFZLV-UHFFFAOYSA-N 0.000 claims description 28
- 239000000047 product Substances 0.000 claims description 21
- 239000007787 solid Substances 0.000 claims description 18
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims description 15
- 150000004682 monohydrates Chemical class 0.000 claims description 13
- 239000002904 solvent Substances 0.000 claims description 12
- 238000001914 filtration Methods 0.000 claims description 10
- LZPZPHGJDAGEJZ-AKAIJSEGSA-N regadenoson Chemical compound C1=C(C(=O)NC)C=NN1C1=NC(N)=C(N=CN2[C@H]3[C@@H]([C@H](O)[C@@H](CO)O3)O)C2=N1 LZPZPHGJDAGEJZ-AKAIJSEGSA-N 0.000 claims description 10
- 238000005406 washing Methods 0.000 claims description 6
- 239000002002 slurry Substances 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 4
- 239000007864 aqueous solution Substances 0.000 claims description 3
- 239000008213 purified water Substances 0.000 claims description 3
- 230000002194 synthesizing effect Effects 0.000 claims description 3
- 238000010792 warming Methods 0.000 claims description 3
- 239000007795 chemical reaction product Substances 0.000 claims 1
- 238000003786 synthesis reaction Methods 0.000 abstract description 13
- 230000015572 biosynthetic process Effects 0.000 abstract description 9
- 238000011031 large-scale manufacturing process Methods 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 description 37
- 239000000203 mixture Substances 0.000 description 24
- 238000002360 preparation method Methods 0.000 description 20
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 14
- 238000004458 analytical method Methods 0.000 description 14
- BAYFDGKAUSOEIS-UUOKFMHZSA-N (2r,3r,4s,5r)-2-(6-amino-2-hydrazinylpurin-9-yl)-5-(hydroxymethyl)oxolane-3,4-diol Chemical compound C12=NC(NN)=NC(N)=C2N=CN1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O BAYFDGKAUSOEIS-UUOKFMHZSA-N 0.000 description 11
- 238000001228 spectrum Methods 0.000 description 11
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 description 10
- HMFLBGNCDZYITR-UHFFFAOYSA-N ethyl 2-formyl-3-oxopropanoate Chemical compound CCOC(=O)C(C=O)C=O HMFLBGNCDZYITR-UHFFFAOYSA-N 0.000 description 10
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 10
- 239000012467 final product Substances 0.000 description 9
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 8
- 239000012535 impurity Substances 0.000 description 8
- 238000003756 stirring Methods 0.000 description 8
- 201000010099 disease Diseases 0.000 description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 7
- IKDUDTNKRLTJSI-UHFFFAOYSA-N hydrazine hydrate Chemical compound O.NN IKDUDTNKRLTJSI-UHFFFAOYSA-N 0.000 description 7
- 239000000463 material Substances 0.000 description 7
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 6
- 238000002441 X-ray diffraction Methods 0.000 description 6
- 239000008367 deionised water Substances 0.000 description 6
- 229910021641 deionized water Inorganic materials 0.000 description 6
- 238000000634 powder X-ray diffraction Methods 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 239000002126 C01EB10 - Adenosine Substances 0.000 description 5
- 229960005305 adenosine Drugs 0.000 description 5
- 239000007789 gas Substances 0.000 description 5
- 239000003586 protic polar solvent Substances 0.000 description 5
- 238000000746 purification Methods 0.000 description 5
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- IAZDPXIOMUYVGZ-WFGJKAKNSA-N Dimethyl sulfoxide Chemical compound [2H]C([2H])([2H])S(=O)C([2H])([2H])[2H] IAZDPXIOMUYVGZ-WFGJKAKNSA-N 0.000 description 4
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- WYURNTSHIVDZCO-UHFFFAOYSA-N Tetrahydrofuran Chemical compound C1CCOC1 WYURNTSHIVDZCO-UHFFFAOYSA-N 0.000 description 4
- -1 coatings Substances 0.000 description 4
- 229910052757 nitrogen Inorganic materials 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- BIXYYZIIJIXVFW-UUOKFMHZSA-N (2R,3R,4S,5R)-2-(6-amino-2-chloro-9-purinyl)-5-(hydroxymethyl)oxolane-3,4-diol Chemical compound C1=NC=2C(N)=NC(Cl)=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O BIXYYZIIJIXVFW-UUOKFMHZSA-N 0.000 description 3
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
- 238000005481 NMR spectroscopy Methods 0.000 description 3
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 3
- 150000001344 alkene derivatives Chemical class 0.000 description 3
- 238000001460 carbon-13 nuclear magnetic resonance spectrum Methods 0.000 description 3
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- 230000005855 radiation Effects 0.000 description 3
- 229910052710 silicon Inorganic materials 0.000 description 3
- 239000010703 silicon Substances 0.000 description 3
- 229910000104 sodium hydride Inorganic materials 0.000 description 3
- 239000007858 starting material Substances 0.000 description 3
- 208000024891 symptom Diseases 0.000 description 3
- CDQVVPUXSPZONN-WPPLYIOHSA-N 1-[6-amino-9-[(2r,3r,4s,5r)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]purin-2-yl]-n-methylpyrazole-4-carboxamide;hydrate Chemical compound O.C1=C(C(=O)NC)C=NN1C1=NC(N)=C(N=CN2[C@H]3[C@@H]([C@H](O)[C@@H](CO)O3)O)C2=N1 CDQVVPUXSPZONN-WPPLYIOHSA-N 0.000 description 2
- XKRFYHLGVUSROY-UHFFFAOYSA-N Argon Chemical compound [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 description 2
- 229910002483 Cu Ka Inorganic materials 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- OAKJQQAXSVQMHS-UHFFFAOYSA-N Hydrazine Chemical compound NN OAKJQQAXSVQMHS-UHFFFAOYSA-N 0.000 description 2
- 241000124008 Mammalia Species 0.000 description 2
- ATHHXGZTWNVVOU-UHFFFAOYSA-N N-methylformamide Chemical compound CNC=O ATHHXGZTWNVVOU-UHFFFAOYSA-N 0.000 description 2
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 2
- XBDQKXXYIPTUBI-UHFFFAOYSA-M Propionate Chemical compound CCC([O-])=O XBDQKXXYIPTUBI-UHFFFAOYSA-M 0.000 description 2
- KEAYESYHFKHZAL-UHFFFAOYSA-N Sodium Chemical compound [Na] KEAYESYHFKHZAL-UHFFFAOYSA-N 0.000 description 2
- 239000004480 active ingredient Substances 0.000 description 2
- 229910052782 aluminium Inorganic materials 0.000 description 2
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 2
- 239000008346 aqueous phase Substances 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000004071 biological effect Effects 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 208000029078 coronary artery disease Diseases 0.000 description 2
- 239000003218 coronary vasodilator agent Substances 0.000 description 2
- 239000013078 crystal Substances 0.000 description 2
- 238000000921 elemental analysis Methods 0.000 description 2
- SIALOQYKFQEKOG-UHFFFAOYSA-N ethyl 3,3-diethoxypropanoate Chemical compound CCOC(OCC)CC(=O)OCC SIALOQYKFQEKOG-UHFFFAOYSA-N 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 239000012065 filter cake Substances 0.000 description 2
- WBJINCZRORDGAQ-UHFFFAOYSA-N formic acid ethyl ester Natural products CCOC=O WBJINCZRORDGAQ-UHFFFAOYSA-N 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 238000004128 high performance liquid chromatography Methods 0.000 description 2
- 238000003384 imaging method Methods 0.000 description 2
- 238000002844 melting Methods 0.000 description 2
- 230000008018 melting Effects 0.000 description 2
- 239000012044 organic layer Substances 0.000 description 2
- IOLCXVTUBQKXJR-UHFFFAOYSA-M potassium bromide Chemical compound [K+].[Br-] IOLCXVTUBQKXJR-UHFFFAOYSA-M 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000012312 sodium hydride Substances 0.000 description 2
- 239000011343 solid material Substances 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 230000003595 spectral effect Effects 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- YLQBMQCUIZJEEH-UHFFFAOYSA-N tetrahydrofuran Natural products C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 description 2
- 238000002076 thermal analysis method Methods 0.000 description 2
- 230000024883 vasodilation Effects 0.000 description 2
- NWZSZGALRFJKBT-KNIFDHDWSA-N (2s)-2,6-diaminohexanoic acid;(2s)-2-hydroxybutanedioic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O.NCCCC[C@H](N)C(O)=O NWZSZGALRFJKBT-KNIFDHDWSA-N 0.000 description 1
- BHKKSKOHRFHHIN-MRVPVSSYSA-N 1-[[2-[(1R)-1-aminoethyl]-4-chlorophenyl]methyl]-2-sulfanylidene-5H-pyrrolo[3,2-d]pyrimidin-4-one Chemical compound N[C@H](C)C1=C(CN2C(NC(C3=C2C=CN3)=O)=S)C=CC(=C1)Cl BHKKSKOHRFHHIN-MRVPVSSYSA-N 0.000 description 1
- 108050000203 Adenosine receptors Proteins 0.000 description 1
- 102000009346 Adenosine receptors Human genes 0.000 description 1
- 238000005033 Fourier transform infrared spectroscopy Methods 0.000 description 1
- 238000004566 IR spectroscopy Methods 0.000 description 1
- 241001347978 Major minor Species 0.000 description 1
- BZLVMXJERCGZMT-UHFFFAOYSA-N Methyl tert-butyl ether Chemical compound COC(C)(C)C BZLVMXJERCGZMT-UHFFFAOYSA-N 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 1
- 229920003350 Spectratech® Polymers 0.000 description 1
- RHQDFWAXVIIEBN-UHFFFAOYSA-N Trifluoroethanol Chemical compound OCC(F)(F)F RHQDFWAXVIIEBN-UHFFFAOYSA-N 0.000 description 1
- 239000003070 absorption delaying agent Substances 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 230000008484 agonism Effects 0.000 description 1
- 238000004164 analytical calibration Methods 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 229940121375 antifungal agent Drugs 0.000 description 1
- 239000003429 antifungal agent Substances 0.000 description 1
- 229910052786 argon Inorganic materials 0.000 description 1
- VSQLEUYPHBCPMK-UHFFFAOYSA-N argon ethyl 3,3-diethoxypropanoate Chemical compound C(C)OC(CC(=O)OCC)OCC.[Ar] VSQLEUYPHBCPMK-UHFFFAOYSA-N 0.000 description 1
- 230000000747 cardiac effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 210000004351 coronary vessel Anatomy 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 238000003795 desorption Methods 0.000 description 1
- 238000000113 differential scanning calorimetry Methods 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 239000002612 dispersion medium Substances 0.000 description 1
- 239000003937 drug carrier Substances 0.000 description 1
- 238000011067 equilibration Methods 0.000 description 1
- ZPVLTIXYQGANFL-IDTAVKCVSA-N ethyl 1-[6-amino-9-[(2r,3r,4s,5r)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]purin-2-yl]pyrazole-4-carboxylate Chemical compound C1=C(C(=O)OCC)C=NN1C1=NC(N)=C(N=CN2[C@H]3[C@@H]([C@H](O)[C@@H](CO)O3)O)C2=N1 ZPVLTIXYQGANFL-IDTAVKCVSA-N 0.000 description 1
- SIZVDXCOFBYELW-QGOBPSGUSA-N ethyl 5-[9-[(2r,3r,4s,5r)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-6-[[(e)-3-ethoxy-2-formyl-3-oxoprop-1-enyl]amino]purin-2-yl]-1h-pyrazole-4-carboxylate Chemical compound C1=NC=2C(N/C=C(\C=O)C(=O)OCC)=NC(C=3C(=CNN=3)C(=O)OCC)=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O SIZVDXCOFBYELW-QGOBPSGUSA-N 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 239000005338 frosted glass Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 150000004677 hydrates Chemical class 0.000 description 1
- 239000005457 ice water Substances 0.000 description 1
- 239000012216 imaging agent Substances 0.000 description 1
- 229910052738 indium Inorganic materials 0.000 description 1
- APFVFJFRJDLVQX-UHFFFAOYSA-N indium atom Chemical compound [In] APFVFJFRJDLVQX-UHFFFAOYSA-N 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 238000002329 infrared spectrum Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 239000007951 isotonicity adjuster Substances 0.000 description 1
- 239000010410 layer Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- USTIRCKMQGZABE-ZBMQJGODSA-N n-[1-[6-amino-9-[(2r,3r,4s,5r)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]purin-2-yl]pyrazol-4-yl]-n-methylformamide;hydrate Chemical compound O.C1=C(N(C=O)C)C=NN1C1=NC(N)=C(N=CN2[C@H]3[C@@H]([C@H](O)[C@@H](CO)O3)O)C2=N1 USTIRCKMQGZABE-ZBMQJGODSA-N 0.000 description 1
- 229910052759 nickel Inorganic materials 0.000 description 1
- 231100000957 no side effect Toxicity 0.000 description 1
- 238000000655 nuclear magnetic resonance spectrum Methods 0.000 description 1
- 239000002777 nucleoside Substances 0.000 description 1
- 150000003833 nucleoside derivatives Chemical class 0.000 description 1
- 230000020477 pH reduction Effects 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 239000002798 polar solvent Substances 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 238000011045 prefiltration Methods 0.000 description 1
- 125000006239 protecting group Chemical group 0.000 description 1
- 238000010926 purge Methods 0.000 description 1
- 239000012264 purified product Substances 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- 235000011152 sodium sulphate Nutrition 0.000 description 1
- 239000012265 solid product Substances 0.000 description 1
- 239000012453 solvate Substances 0.000 description 1
- 238000002336 sorption--desorption measurement Methods 0.000 description 1
- 238000009987 spinning Methods 0.000 description 1
- 238000010189 synthetic method Methods 0.000 description 1
- CZDYPVPMEAXLPK-UHFFFAOYSA-N tetramethylsilane Chemical compound C[Si](C)(C)C CZDYPVPMEAXLPK-UHFFFAOYSA-N 0.000 description 1
- BKVIYDNLLOSFOA-OIOBTWANSA-N thallium-201 Chemical compound [201Tl] BKVIYDNLLOSFOA-OIOBTWANSA-N 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- GZXOHHPYODFEGO-UHFFFAOYSA-N triglycine sulfate Chemical class NCC(O)=O.NCC(O)=O.NCC(O)=O.OS(O)(=O)=O GZXOHHPYODFEGO-UHFFFAOYSA-N 0.000 description 1
- 238000003828 vacuum filtration Methods 0.000 description 1
Landscapes
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Nitrogen Condensed Heterocyclic Rings (AREA)
Abstract
Disclosed is a synthesis suitable for large scale manufacture of an A2A- adenosine receptor agonist, and also relates to polymorphs of the compound: (see above formula) and to methods of isolating a specific polymorph.
Description
ITS POLYMORPHS
(00011 Field of the Invention
(00011 Field of the Invention
[0002] The present invention relates to a process for the large scale preparation of an A2A-adenosine receptor agonist, and also relates to polymorphs of that compound, and to methods of isolating a specific polymorph.
Background
Background
[0003] Adenosine is a naturally occurring nucleoside, which exerts its biological effects by interacting with a family of adenosine receptors known as Ai, A2A, A25, and A3, all of which modulate important physiological processes. One of the biological effects of adenosine is to act as a coronary vasodilator; this result being produced by interaction with the A2A adenosine receptor. This effect of adenosine has been found to be useful as an aid to imaging of the heart, where coronary arteries are dilated prior to administration of an imaging agent (for example thallium 201), and thus, by observation of the images thus produced, the presence or absence of coronary artery disease can be determined. The advantage of such a technique is that it avoids the more traditional method of inducing coronary vasodilation by exercise on a treadmill, which is clearly undesirable for a patient that has a coronary disease.
[0004] However, administration of adenosine has several disadvantages.
Adenosine has a very short half life in humans (less than 10 seconds), and also has all of the effects associated with A,, A2A, A2B, and A3 receptor agonism. Thus the use of a selective A2A adenosine receptor agonist would provide a superior method of producing coronary vasodilation, particularly one with a longer half life and few or no side effects.
[00051 A class of compounds possessing these desirable properties was disclosed in U.S. Patent No. 6,403,567.
In particular, one compound disclosed in this patent, (1-{9-[(4S,2R,3R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-6-aminopurin-2-yI } pyrazo l-4-yl)-N-methylcarboxamide, has been shown to be a highly selective A2A-adenosine receptor agonist, and is presently undergoing clinical trials as a coronary vasodilator useful in cardiac imaging.
[00061 Given the heightened interest in this and similar compounds, it has become desirable to find new methods of synthesis that provide a convenient method for making large quantities of the material in good yield and high purity. The patent that discloses the compound of interest (U-S. Patent No. 6,403,567) provides several methods for preparing the compound. However, although these methods are suited to small scale syntheses, all synthetic methods disclosed in the patent utilize protecting groups, which is undesirable for large scale syntheses.
[00071 Additionally, it was discovered that the desired product (that is (1-{9-[(4S,2R,3R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-6-aminopurin-2-yl}pyrazol-4-yl) N-methylcarboxamide) is capable of existing in at least three different crystalline forms, the most stable of which is a monohydrate. This polymorph is stable under relative humidity stress conditions, up to its melting point-Accordingly, it is desirable that the final product produced in the new syntheses is obtained as the stable monohydrate.
SUMMARY OF THE INVENTION
[00081 Thus, it is an object of this invention to provide convenient syntheses for the large scale preparation of (I- {9-[(4S,2R,3R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-6-aminopurin-2-yl } pyrazol-4-yl)-N-methylcarboxamide, and polymorphs thereof, preferably as its monohydrate. Accordingly, in a first aspect, the invention relates to the preparation of a compound of the Formula I:
NH, N N
O 1 / \
--..._NH ~.
~'Q
OH
H
Formula I
comprising:
contacting a compound of the formula (3):
NHi N
N ~I
N N
---OH
HO
(3) with methylamine_ [00091 In one embodiment the reaction is conducted in an aqueous solution of methylamine, initially at a temperature of about 0-5 C, followed by warming to about 50-70 C. Alternatively, the reaction is conducted as above but in a sealed pressure reactor.
[00101 In a second embodiment, the product is isolated as the pure monohydrate by dissolving the product in a solvent, for example dimethylsulfoxide, addition of purified water, filtering the slurry thus formed, washing the contents of the filter with water followed by ethanol, and drying the solid that remains under vacuum at a temperature that does not exceed 40 C.
100111 In a second aspect, the invention relates to the preparation of a compound of the formula (3):
N
N
o JI \
/ i N N
,OH
O
~~OH
'0 (3) comprising:
contacting a compound of the formula (2):
N
~
HZNHN N N
O
Ho-with ethyl 2-fommyl-3-oxopropionate.
100121 In one embodiment, the reaction is conducted in ethanol, at a temperature of about 80 C, with about 1.1 molar equivalents of ethyl 2-formyl-3-oxopropionate.
[0013) In a third aspect, the invention relates to the preparation of a compound of the formula (2):
NH, N
H,NHN N O
~~OH
Ho-(2) comprising:
contacting a compound of the formula (1):
N ~I
d N N
O
,111-OH
HO
(1) with hydrazine.
[0014] The above described synthesis is suitable for the large scale synthesis of the desired product, which is provided in good yield, although one minor impurity is seen in the final product. This impurity has been shown to be unchanged intermediate of the formula (2); that is, the compound of the formula:
1 ` N
NOH
O
(2) "OH
HO
Although this impurity can be removed from the final product by crystallization, it was decided to seek an alternative synthesis that had all of the advantages of the above synthesis but did not give the compound of formula (2) as an impurity in the final product.
[0015] Thus, in a fourth aspect, the invention relates to a method of synthesizing (I - {9-[(4S,2R,3R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-6-aminopurin-2-yl}pyrazol-4-yl)-N-methylcarboxamide by contacting a compound of the formula (4):
= - 5 H N~
O ~
/ N,/~N N
``SOH
-~N
~OH
H
(4) with methylamine.
[0016] In one embodiment the reaction is conducted in an aqueous solution of methylamine, initially at a temperature of about 0-5 C, followed by warming to about 50-70 C. Preferably, the reaction is conducted in a sealed pressure reactor.
[0017] In a second embodiment, the product is isolated as the pure monohydrate by dissolving the product in a solvent, for example dimethylsulfoxide, addition of purified water, filtering the slurry thus formed, washing the contents of the filter with water followed by ethanol, and drying the solid that remains under vacuum at a temperature that does not exceed 40 C.
[0018] In a fi$h aspect, the invention relates to a method of synthesizing a compound of the formula (4):
/ ` H
N
! N
N
)-Cr N
O HO~
(4) comprising contacting a compound of the formula (2):
N
N
O
~~OH
HO
(2) with an excess of ethyl 2-formyl-3-oxopropionate, preferably about a 2-10 fold excess, more preferably about a 5-10 fold excess.
[0019] In one embodiment, the reaction is conducted in ethanol, at a temperature of about 80 C. The ethyl 2-formyl-3-oxopropionate is present in a 5-10 fold excess.
Definitions and General Parameters [0020] FIGURE 1 is a 1H NMR spectrum of (1.={9-[(4S,2R,3R,5R)-3,4-dihydroxy=
Adenosine has a very short half life in humans (less than 10 seconds), and also has all of the effects associated with A,, A2A, A2B, and A3 receptor agonism. Thus the use of a selective A2A adenosine receptor agonist would provide a superior method of producing coronary vasodilation, particularly one with a longer half life and few or no side effects.
[00051 A class of compounds possessing these desirable properties was disclosed in U.S. Patent No. 6,403,567.
In particular, one compound disclosed in this patent, (1-{9-[(4S,2R,3R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-6-aminopurin-2-yI } pyrazo l-4-yl)-N-methylcarboxamide, has been shown to be a highly selective A2A-adenosine receptor agonist, and is presently undergoing clinical trials as a coronary vasodilator useful in cardiac imaging.
[00061 Given the heightened interest in this and similar compounds, it has become desirable to find new methods of synthesis that provide a convenient method for making large quantities of the material in good yield and high purity. The patent that discloses the compound of interest (U-S. Patent No. 6,403,567) provides several methods for preparing the compound. However, although these methods are suited to small scale syntheses, all synthetic methods disclosed in the patent utilize protecting groups, which is undesirable for large scale syntheses.
[00071 Additionally, it was discovered that the desired product (that is (1-{9-[(4S,2R,3R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-6-aminopurin-2-yl}pyrazol-4-yl) N-methylcarboxamide) is capable of existing in at least three different crystalline forms, the most stable of which is a monohydrate. This polymorph is stable under relative humidity stress conditions, up to its melting point-Accordingly, it is desirable that the final product produced in the new syntheses is obtained as the stable monohydrate.
SUMMARY OF THE INVENTION
[00081 Thus, it is an object of this invention to provide convenient syntheses for the large scale preparation of (I- {9-[(4S,2R,3R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-6-aminopurin-2-yl } pyrazol-4-yl)-N-methylcarboxamide, and polymorphs thereof, preferably as its monohydrate. Accordingly, in a first aspect, the invention relates to the preparation of a compound of the Formula I:
NH, N N
O 1 / \
--..._NH ~.
~'Q
OH
H
Formula I
comprising:
contacting a compound of the formula (3):
NHi N
N ~I
N N
---OH
HO
(3) with methylamine_ [00091 In one embodiment the reaction is conducted in an aqueous solution of methylamine, initially at a temperature of about 0-5 C, followed by warming to about 50-70 C. Alternatively, the reaction is conducted as above but in a sealed pressure reactor.
[00101 In a second embodiment, the product is isolated as the pure monohydrate by dissolving the product in a solvent, for example dimethylsulfoxide, addition of purified water, filtering the slurry thus formed, washing the contents of the filter with water followed by ethanol, and drying the solid that remains under vacuum at a temperature that does not exceed 40 C.
100111 In a second aspect, the invention relates to the preparation of a compound of the formula (3):
N
N
o JI \
/ i N N
,OH
O
~~OH
'0 (3) comprising:
contacting a compound of the formula (2):
N
~
HZNHN N N
O
Ho-with ethyl 2-fommyl-3-oxopropionate.
100121 In one embodiment, the reaction is conducted in ethanol, at a temperature of about 80 C, with about 1.1 molar equivalents of ethyl 2-formyl-3-oxopropionate.
[0013) In a third aspect, the invention relates to the preparation of a compound of the formula (2):
NH, N
H,NHN N O
~~OH
Ho-(2) comprising:
contacting a compound of the formula (1):
N ~I
d N N
O
,111-OH
HO
(1) with hydrazine.
[0014] The above described synthesis is suitable for the large scale synthesis of the desired product, which is provided in good yield, although one minor impurity is seen in the final product. This impurity has been shown to be unchanged intermediate of the formula (2); that is, the compound of the formula:
1 ` N
NOH
O
(2) "OH
HO
Although this impurity can be removed from the final product by crystallization, it was decided to seek an alternative synthesis that had all of the advantages of the above synthesis but did not give the compound of formula (2) as an impurity in the final product.
[0015] Thus, in a fourth aspect, the invention relates to a method of synthesizing (I - {9-[(4S,2R,3R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-6-aminopurin-2-yl}pyrazol-4-yl)-N-methylcarboxamide by contacting a compound of the formula (4):
= - 5 H N~
O ~
/ N,/~N N
``SOH
-~N
~OH
H
(4) with methylamine.
[0016] In one embodiment the reaction is conducted in an aqueous solution of methylamine, initially at a temperature of about 0-5 C, followed by warming to about 50-70 C. Preferably, the reaction is conducted in a sealed pressure reactor.
[0017] In a second embodiment, the product is isolated as the pure monohydrate by dissolving the product in a solvent, for example dimethylsulfoxide, addition of purified water, filtering the slurry thus formed, washing the contents of the filter with water followed by ethanol, and drying the solid that remains under vacuum at a temperature that does not exceed 40 C.
[0018] In a fi$h aspect, the invention relates to a method of synthesizing a compound of the formula (4):
/ ` H
N
! N
N
)-Cr N
O HO~
(4) comprising contacting a compound of the formula (2):
N
N
O
~~OH
HO
(2) with an excess of ethyl 2-formyl-3-oxopropionate, preferably about a 2-10 fold excess, more preferably about a 5-10 fold excess.
[0019] In one embodiment, the reaction is conducted in ethanol, at a temperature of about 80 C. The ethyl 2-formyl-3-oxopropionate is present in a 5-10 fold excess.
Definitions and General Parameters [0020] FIGURE 1 is a 1H NMR spectrum of (1.={9-[(4S,2R,3R,5R)-3,4-dihydroxy=
5-(hydroxymethyl)oxolan-2-yl]-6-aminopurin-2-y1} pyrazol-4-yl)-N-methylearboxamide monohydrate (Form A).
[0021] FIGURE 2 shows the thermal analysis of(1-{9-[(4S,2R,3R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-=ylj-6-aminopurin-2-yl}pyrazol-4-yl)-N-methylcarboxamide monohydrate.
[0022] FIGURE 3 shows the X-Ray diffraction pattern for (1-{9-[(4S,2R,3R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-6-aminopurin--2-yl)pyrazol-4-yl) N-methylcarboxamide monohydrate.
[0023] FIGURE 4 shows the X-Ray diffraction pattern for (1-{9-[(4S,2R,3R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-ylj-6-aminopurin-2-yl}pyrazol-4-yl)-N-methylcarboxamide Form B.
[0024] FIGURE 5 shows the X-Ray diffraction pattern for (1 - {9-[(4S,2R,3R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-ylj-6-aminopurin-2-yl } p yrazol--4-yl)-N-methylcarboxamide Form C as compared to Form A.
[0025] As used in the present specification, the following words and phrases are generally intended to have the meanings as set forth below, except to the extent that the context in which they are used indicates otherwise.
(0026] "Optional" or "optionally" means that the subsequently described event or circumstance may or may not occur, and that the description includes instances where said event or circumstance occurs and instances in which it does not.
[0027] The term "therapeutically effective amount" refers to that amount of a compound of Formula I that is sufficient to effect treatment, as defined below, when administered to a mammal in need of such treatment. The therapeutically effective amount will vary depending upon the subject and disease condition being treated, the weight and age of the subject, the severity of the disease condition, the manner of administration and the like, which can readily be determined by one of ordinary skill in the art.
[0028] The term "treatment" or "treating" means any treatment of a disease in a mammal, including:
(i) preventing the disease, that is, causing the clinical symptoms of the disease not to develop;
(ii) inhibiting the disease, that is, arresting the development of clinical symptoms;
and/or (iii)relieving the disease, that is, causing the regression of clinical symptoms.
[0029] As used herein, "pharmaceutically acceptable carrier" includes any and all solvents, dispersion media, coatings, antibacterial and antifungal agents, isotonic and absorption delaying agents and the like. The use of such media and agents for pharmaceutically active substances is well known in the art. Except insofar as any conventional media or agent is incompatible with the active ingredient, its use in the therapeutic compositions is contemplated. Supplementary active ingredients can also be incorporated into the compositions.
[0030] The term "polymorph" is intended to include amorphous and solvates of (1-{9-[(4S,2R,3R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl)-6-aminopurin-2-yl} pyrazol-4-yl)-N-methylcarboxamide.
[0031] It has been discovered that this compound is capable of existing in at least three different crystalline forms, referred to herein as Form A, Form B, Form C, and an amorphous product.
[00321 Form A: This polymorph can be produced by crystallizing 1-{9-[(4S,2R,3R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-6-aminopurin-2-yl} pyrazol-4-yl)-N-methylcarboxamide from protic solvents, for example ethanol or ethanol/water mixtures, or from a polar solvent, for example dimethylsulfoxide/water.
Form A has been shown to be a monohydrate, and is the most stable of the various polymorphs at ambient temperatures. It is stable under relative humidity stress conditions up to its melting point.
10033] Form B: This polymorph is produced by evaporating under vacuum a solution of I- (9-[(4S,2R,3R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-6-aminopurin-2-yi}pyrazol-4-yl)-N-methylcarboxamide in trifluoroethanol at ambient temperatures. The X-ray analysis of the crystals was distinctly different from any other polymorph (see Figure 4), but it was difficult to determine its constitution, as the X-ray analysis gave disordered broad peaks, and the polymorph contained varying amounts of water. It was found to be difficult to reliably reproduce the preparation of this polymorph.
[0034] Form C: This polymorph is produced by slurrying 1-{9-[(4S,2R,3R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-6-aminopurin-2-yl}pyrazol-4-yl) N-methylcarboxamide in acetonitrile for a long period of time at 60 C. The X-ray analysis of the crystals was distinctly different from any other polymorph (see Figure 5). Folyrnorph C was shown to be a variable hydrate, which, at elevated temperatures, desolvates to an unstable form.
[00351 Amorphous Material: This polymorph is produced by heating Form A
polymorph at a temperature of up to 200 C. This polymorph is unstable in the presence of atmospheric moisture, forming variable hydrates.
Techniques for Analysis of Forms A,- B, C and Amorpheous Material X-Rgy Powder Diffraction 10036] X-ray powder diffraction (XRPD) analyses were carried out on a Shimadzu XRD-6000 X-ray powder diffractometer using Cu Ka radiation. The instrument was equipped with a fine focus X-ray tube, and the tube voltage and amperage were set to 40 kV and 40 mA respectively. The divergence and scattering slits were set at 1" and the receiving slit was set at 0.15 mm. Diffracted radiation was detected by a Nal scintillation detector. A theta-two theta continuous scan at 3 /min (0.4 sec/0.02 step) from 2.5-40 20 was used. A silicon standard was used to check the instrument alignment. Data were collected and analyzed using XRD-6000 v. 4.1 software.
[0037] X-ray powder diffraction (XRPD) analyses were also performed using an Teel XRG-3000 diffractometer equipped with a CPS (Curved Position Sensitive) detector with a 28 range of 120 . The instrument calibration was performed using a silicon reference standard. The tube voltage and amperage were set to 40 kV
and.30 mA, respectively. The monochromator slit was set at 5 mm by 80 jim. Samples were placed in an aluminum sample holder with a silicon insert or in glass XRPD-quality capillaries. Each capillary was mounted onto a goniometer head that is motorized to permit spinning of the capillary during data acquisition. Real time data were collected using Cu-Ka radiation at a resolution of 0.03 20. Typically, data were collected over a period of 300 seconds. Only the data points within the range of 2.5-40 20 are displayed in the plotted XRPD patterns.
Thermal Analyses 10038] Thermogravimetric (TG) analyses were carried out on a TA Instruments 2050 or 2950 thermogravimetric analyzer. The calibration standards were nickel and AlumelTM. Samples were placed in an aluminum sample pan, inserted into the TG
fiunace, and accurately weighed. The samples were heated in nitrogen at a rate of C/min to either 300 or 350 C. Unless stated otherwise, samples weights were equilibrated at 25 C in the TGA furnace prior to analysis.
10039] Differential scanning calorimetry (DSC) analyses were carried out on a TA
Instruments differential scanning calorimeter 2920. Accurately weighed samples were placed in either crimped pans or hermetically sealed pans that contained a pinhole to allow for pressure release. Each sample was heated under nitrogen at a rate of C/min to either 300 or 350 C. Indium metal was used as the calibration standard.
Temperatures were reported at the transition maxima.
Infrared Spectroscopy [0040] Infrared spectra were acquired on Magna 8600 Fourier transform infrared (FT-IR) spectrophotometer (Nicolet Instrument Corp.) equipped with an Ever-Glo M
mid/far IR source, an extended range potassium bromide beamsplitter, and a deuterated triglycine sulfate (DTGS) detector- Unless stated otherwise, a Spectra-Tech, Inc.
diffuse reflectance accessory (the CollectorTM) was used for sampling. Each spectrum represents 256 co-added scans at a spectral resolution of 4 cm i. Sample preparation for the compound consisted of placing the sample into a microcup and leveling the material with a. frosted glass slide. A background data set was acquired with an alignment mirror in place. The spectra represent a ratio of the sample single-beam data set to the background single beam data set- Wavelength calibration of the instrument was performed using polystyrene.
NMR Spectroscopy (0041] Solution phase 'H NMR spectra of the were acquired at ambient temperature on a Bruker model AM-250 spectrometer operating at 5.87 T (Larmor frequency:
'H =
250 MHz). Time-domain data were acquired using a pulse width of 7.5 ps and an acquisition time of 1.6834 second over a spectral window of 5000 Hz. A total of 16,384 data points were collected. A relaxation delay time of 5 seconds was employed between transients. Each data set typically consisted of 128 coaveraged transients.
The spectra TM
were processed utilizing GRAMS 132 Al software, version 6.00. The free induction decay (FID) was zero-filled to four times the number of data points and exponentially multiplied with a line-broadening factor of 0.61 Hz prior to Fourier transformation.
The 'H spectra were internally referenced to tetramethylsilane (0 ppm) that was added as an internal standard.
[0042] Alternatively, NMR analysis was carried out as described in Example 4.
Moisture SorptionJDesorption Analyses TM
[0043] Moisture sorption/desorption data were collected on a VTI SGA-100 Vapor Sorption Analyzer. Sorption and desorption data were collected over a range of 5% to 95% relative humidity (RK) at 10% RH intervals under a nitrogen purge. Sodium chloride (NaCl) and polyvinyllpyrrolidone (PVP) were used as the calibration standards. Equilibrium criteria used for analysis were less than 0.0100%
weight change in 5 minutes, with a maximum equilibration time of 180 minutes if the weight criterion was not met. The plotted data have not been corrected for the initial moisture content.
Nomenclature [0044] The structure of the compound (1-{9-[(4S,2R,3R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-6-aminopurin-2-yl} pyrazol-4-yl)-N-methylcarboxamide is as follows:
Nfl, N
J-1-, NH ~N
O
""'-'OH
HO
Synthesis of(1- f9-f(4S,2R,3R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-y1]-
[0021] FIGURE 2 shows the thermal analysis of(1-{9-[(4S,2R,3R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-=ylj-6-aminopurin-2-yl}pyrazol-4-yl)-N-methylcarboxamide monohydrate.
[0022] FIGURE 3 shows the X-Ray diffraction pattern for (1-{9-[(4S,2R,3R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-6-aminopurin--2-yl)pyrazol-4-yl) N-methylcarboxamide monohydrate.
[0023] FIGURE 4 shows the X-Ray diffraction pattern for (1-{9-[(4S,2R,3R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-ylj-6-aminopurin-2-yl}pyrazol-4-yl)-N-methylcarboxamide Form B.
[0024] FIGURE 5 shows the X-Ray diffraction pattern for (1 - {9-[(4S,2R,3R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-ylj-6-aminopurin-2-yl } p yrazol--4-yl)-N-methylcarboxamide Form C as compared to Form A.
[0025] As used in the present specification, the following words and phrases are generally intended to have the meanings as set forth below, except to the extent that the context in which they are used indicates otherwise.
(0026] "Optional" or "optionally" means that the subsequently described event or circumstance may or may not occur, and that the description includes instances where said event or circumstance occurs and instances in which it does not.
[0027] The term "therapeutically effective amount" refers to that amount of a compound of Formula I that is sufficient to effect treatment, as defined below, when administered to a mammal in need of such treatment. The therapeutically effective amount will vary depending upon the subject and disease condition being treated, the weight and age of the subject, the severity of the disease condition, the manner of administration and the like, which can readily be determined by one of ordinary skill in the art.
[0028] The term "treatment" or "treating" means any treatment of a disease in a mammal, including:
(i) preventing the disease, that is, causing the clinical symptoms of the disease not to develop;
(ii) inhibiting the disease, that is, arresting the development of clinical symptoms;
and/or (iii)relieving the disease, that is, causing the regression of clinical symptoms.
[0029] As used herein, "pharmaceutically acceptable carrier" includes any and all solvents, dispersion media, coatings, antibacterial and antifungal agents, isotonic and absorption delaying agents and the like. The use of such media and agents for pharmaceutically active substances is well known in the art. Except insofar as any conventional media or agent is incompatible with the active ingredient, its use in the therapeutic compositions is contemplated. Supplementary active ingredients can also be incorporated into the compositions.
[0030] The term "polymorph" is intended to include amorphous and solvates of (1-{9-[(4S,2R,3R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl)-6-aminopurin-2-yl} pyrazol-4-yl)-N-methylcarboxamide.
[0031] It has been discovered that this compound is capable of existing in at least three different crystalline forms, referred to herein as Form A, Form B, Form C, and an amorphous product.
[00321 Form A: This polymorph can be produced by crystallizing 1-{9-[(4S,2R,3R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-6-aminopurin-2-yl} pyrazol-4-yl)-N-methylcarboxamide from protic solvents, for example ethanol or ethanol/water mixtures, or from a polar solvent, for example dimethylsulfoxide/water.
Form A has been shown to be a monohydrate, and is the most stable of the various polymorphs at ambient temperatures. It is stable under relative humidity stress conditions up to its melting point.
10033] Form B: This polymorph is produced by evaporating under vacuum a solution of I- (9-[(4S,2R,3R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-6-aminopurin-2-yi}pyrazol-4-yl)-N-methylcarboxamide in trifluoroethanol at ambient temperatures. The X-ray analysis of the crystals was distinctly different from any other polymorph (see Figure 4), but it was difficult to determine its constitution, as the X-ray analysis gave disordered broad peaks, and the polymorph contained varying amounts of water. It was found to be difficult to reliably reproduce the preparation of this polymorph.
[0034] Form C: This polymorph is produced by slurrying 1-{9-[(4S,2R,3R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-6-aminopurin-2-yl}pyrazol-4-yl) N-methylcarboxamide in acetonitrile for a long period of time at 60 C. The X-ray analysis of the crystals was distinctly different from any other polymorph (see Figure 5). Folyrnorph C was shown to be a variable hydrate, which, at elevated temperatures, desolvates to an unstable form.
[00351 Amorphous Material: This polymorph is produced by heating Form A
polymorph at a temperature of up to 200 C. This polymorph is unstable in the presence of atmospheric moisture, forming variable hydrates.
Techniques for Analysis of Forms A,- B, C and Amorpheous Material X-Rgy Powder Diffraction 10036] X-ray powder diffraction (XRPD) analyses were carried out on a Shimadzu XRD-6000 X-ray powder diffractometer using Cu Ka radiation. The instrument was equipped with a fine focus X-ray tube, and the tube voltage and amperage were set to 40 kV and 40 mA respectively. The divergence and scattering slits were set at 1" and the receiving slit was set at 0.15 mm. Diffracted radiation was detected by a Nal scintillation detector. A theta-two theta continuous scan at 3 /min (0.4 sec/0.02 step) from 2.5-40 20 was used. A silicon standard was used to check the instrument alignment. Data were collected and analyzed using XRD-6000 v. 4.1 software.
[0037] X-ray powder diffraction (XRPD) analyses were also performed using an Teel XRG-3000 diffractometer equipped with a CPS (Curved Position Sensitive) detector with a 28 range of 120 . The instrument calibration was performed using a silicon reference standard. The tube voltage and amperage were set to 40 kV
and.30 mA, respectively. The monochromator slit was set at 5 mm by 80 jim. Samples were placed in an aluminum sample holder with a silicon insert or in glass XRPD-quality capillaries. Each capillary was mounted onto a goniometer head that is motorized to permit spinning of the capillary during data acquisition. Real time data were collected using Cu-Ka radiation at a resolution of 0.03 20. Typically, data were collected over a period of 300 seconds. Only the data points within the range of 2.5-40 20 are displayed in the plotted XRPD patterns.
Thermal Analyses 10038] Thermogravimetric (TG) analyses were carried out on a TA Instruments 2050 or 2950 thermogravimetric analyzer. The calibration standards were nickel and AlumelTM. Samples were placed in an aluminum sample pan, inserted into the TG
fiunace, and accurately weighed. The samples were heated in nitrogen at a rate of C/min to either 300 or 350 C. Unless stated otherwise, samples weights were equilibrated at 25 C in the TGA furnace prior to analysis.
10039] Differential scanning calorimetry (DSC) analyses were carried out on a TA
Instruments differential scanning calorimeter 2920. Accurately weighed samples were placed in either crimped pans or hermetically sealed pans that contained a pinhole to allow for pressure release. Each sample was heated under nitrogen at a rate of C/min to either 300 or 350 C. Indium metal was used as the calibration standard.
Temperatures were reported at the transition maxima.
Infrared Spectroscopy [0040] Infrared spectra were acquired on Magna 8600 Fourier transform infrared (FT-IR) spectrophotometer (Nicolet Instrument Corp.) equipped with an Ever-Glo M
mid/far IR source, an extended range potassium bromide beamsplitter, and a deuterated triglycine sulfate (DTGS) detector- Unless stated otherwise, a Spectra-Tech, Inc.
diffuse reflectance accessory (the CollectorTM) was used for sampling. Each spectrum represents 256 co-added scans at a spectral resolution of 4 cm i. Sample preparation for the compound consisted of placing the sample into a microcup and leveling the material with a. frosted glass slide. A background data set was acquired with an alignment mirror in place. The spectra represent a ratio of the sample single-beam data set to the background single beam data set- Wavelength calibration of the instrument was performed using polystyrene.
NMR Spectroscopy (0041] Solution phase 'H NMR spectra of the were acquired at ambient temperature on a Bruker model AM-250 spectrometer operating at 5.87 T (Larmor frequency:
'H =
250 MHz). Time-domain data were acquired using a pulse width of 7.5 ps and an acquisition time of 1.6834 second over a spectral window of 5000 Hz. A total of 16,384 data points were collected. A relaxation delay time of 5 seconds was employed between transients. Each data set typically consisted of 128 coaveraged transients.
The spectra TM
were processed utilizing GRAMS 132 Al software, version 6.00. The free induction decay (FID) was zero-filled to four times the number of data points and exponentially multiplied with a line-broadening factor of 0.61 Hz prior to Fourier transformation.
The 'H spectra were internally referenced to tetramethylsilane (0 ppm) that was added as an internal standard.
[0042] Alternatively, NMR analysis was carried out as described in Example 4.
Moisture SorptionJDesorption Analyses TM
[0043] Moisture sorption/desorption data were collected on a VTI SGA-100 Vapor Sorption Analyzer. Sorption and desorption data were collected over a range of 5% to 95% relative humidity (RK) at 10% RH intervals under a nitrogen purge. Sodium chloride (NaCl) and polyvinyllpyrrolidone (PVP) were used as the calibration standards. Equilibrium criteria used for analysis were less than 0.0100%
weight change in 5 minutes, with a maximum equilibration time of 180 minutes if the weight criterion was not met. The plotted data have not been corrected for the initial moisture content.
Nomenclature [0044] The structure of the compound (1-{9-[(4S,2R,3R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-6-aminopurin-2-yl} pyrazol-4-yl)-N-methylcarboxamide is as follows:
Nfl, N
J-1-, NH ~N
O
""'-'OH
HO
Synthesis of(1- f9-f(4S,2R,3R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-y1]-
6-aminopurin-2-yl}pyrazol-4-yl -N-methylcarboxamide [0045] One method for the large scale synthesis of (I-{9-[(4S,2R,3R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl)-6-aminopurin-2-yl}pyrazol-4-yl)-N-methylcarboxamide is shown in Reaction Scheme I.
REACTION SCHEME I
C02Et HY
H
111111011 where Et is ethyl 0 (2) (l) "'OH "'OH
O
J-11, N ~'~
Eto ~'N
-N
(3) H
H
Step 1 - Preparation of Formula (2) [0046] The compound of formula (2) is prepared from the compound of formula (1) by reaction with hydrazine monohydrate in the absence of a solvent. The reaction is conducted at a temperature of about 40 C plus/minus 5 C. When the reaction is complete, the product of formula (2) is isolated by stirring with a protic solvent in which the compound of formula (2) has limited solubility, for example ethanol or isopropanol. The mixture is stirred for about 1-5 hours, and then filtered.
The solid is purified by stirring with water, filtering, and washing with water followed by isopropanol and dried under vacuum, which is taken to the next step without purification.
Step 2 - Preparation of Formula (3) [0047] The compound of formula (2) is then converted to a compound of formula (3) by reacting with about 1-1.2 molar equivalents of ethyl 2-formyl-3-oxopropionate.
The reaction is conducted in a protic solvent, preferably ethanol, at about reflux temperature, for about 2-4 hours. After cooling, to about 0 C, the solid is filtered off, washed with cold ethanol, and dried under reduced pressure. The product of formula (3) is taken to the next step without purification.
Step 3 - Preparation of Final Product [0048] The final product is prepared from the compound of formula (3) by reacting with methylamine, preferably aqueous methylamine. The reaction is carried out at about room temperature, for about 4 hours. The product of Formula I is isolated by conventional means, for example by filtration, washing the solid with cold ethanol, and drying under reduced pressure.
Preparation of Starting Materials [0049] (4S,2R,3R,5R)-2-(6-amino-2-chloropurin-9-yl)-5-(hydroxymethyl)oxolane-3,4-diol is used as a starting material in step 1. This compound is commercially available.
[0050] Ethyl 2-formyl-3-oxopropanoate is used as a starting material in step 2. It is commercially available, or may be made as shown in Reaction Scheme 11.
REACTION SCHEME II
OR
a NaH
H OEt EtO OEt COZEt where Et is ethyl [0051] Ethyl 3,3-diethoxypropionate is reacted with ethyl formate in the presence of a strong base, preferably sodium hydride. The reaction is carried out at about 0-5 C, for about 24 hours. The product is isolated by conventional means, for example by the addition of water and extraction of impurities with a conventional solvent, for example t-butyhnethyl ether, acidification of the aqueous phase with, for example, hydrochloric acid, followed by extraction with a solvent such as dichloromethane, and removing the solvent from the dried extract under reduced pressure-[00521 A preferred method for the large scale synthesis of (1-{9-[(4S,2R,3R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-6-aminopurin-2-yl} pyrazol-4-yl)-N-methylcarboxamide is shown in Reaction Scheme III.
REACTION SCHEME III
NHZ NHZ
N Et I \ I \ H(H
Cl N N HZNHN N N
H
where Et is ethyl (~) (2) 'OH
H H
NHZ
H =
NCH N CH3NH2 \/\
O
A `\ N N I "No"
N N -NH N
l^-d N
OH
(4) Ho-HO-Step 1 - Preparation of Formula (2) [0053) The compound of formula (2) is prepared from the compound of formula (1) by reaction with hydrazine monohydrate in the absence of a solvent. The reaction is conducted at a temperature of about 45-55 C plus/minus 5 C. When the reaction is complete, the product of formula (2) is isolated by stirring with a protic solvent in which the compound of formula (2) has limited solubility, for example ethanol or isopropanol. The mixture is stirred for about 1-5 hours, and then filtered.
The solid is purified by stirring with water, filtering, and washing with water followed by ethanol or isopropanol and dried under vacuum, which is taken to the next step without purification.
Step 2 - Preparation of Formula (4) 100541 The compound of formula (2) is then converted to a compound of formula (4) by reacting with an excess of ethyl 2-formyl-3-oxopropionate, for example a 2-10 fold excess, preferably about 5-10 fold excess. The reaction is conducted in a protic solvent, for example ethanol, at about reflux temperature, for about 2-4 hours. After cooling, to about 0 C, the solid is filtered off, washed with cold ethanol, and dried under reduced pressure, and the product of formula (4) is taken to the next step without purification.
[0055] The compound of formula (4) is drawn as a (2E) alkene derivative, as this is the major isomer formed in this reaction. However, it should be noted that a significant amount of the (2Z) alkene derivative may also be formed in this reaction; that is:
H O/ \
H NH
N N
O
N
i N
``-NOH
~O /N
~~OH
HO
named as ethyl (2Z)-3-({ 9-[(4S,2R,3R,5R)-3,4-dihydroxy-5-(hydroxymethyl)-oxolan-2-yl]-2-[4-(ethoxycarbonyl)pyrazolyljpurin-6-yl} amino)-2-formylprop-2-enoate.
[0056] Accordingly, although the compound of formula (4) is represented as the (2E) alkene derivative only, the term "compound of formula (4)" is intended to include both the instance where it is solely the (2E) isomer, and the instance where the major portion of the product is the (2E) isomer and a minor portion of the (2Z) isomer is also present. The conversion of the compound of formula (4) to the final product by reaction with methylamine as described in Step 3 proceeds in the same manner whether the compound of formula (4) is present as the (2E) isomer or as a mixture of the (2E) isomer and the (2Z) isomer.
Step 3 - Preparation of Final Product 10057] The final product is prepared from the compound of formula (4) by reacting with methylamine, preferably aqueous methylamine. The reaction is initially carried out at about 0-5 C for about 8 hours, preferably in a pressure reactor, followed by raising the temperature to 50-60 C over about 1 hour, and maintaining the temperature for 15-30 minutes. The product is isolated by conventional means, for example by cooling to 0-5 C and maintaining a vacuum for about 1 hour, thus removing the methylamine. The vacuum is removed, and the remaining contents held at 0-5 C
for at least 30 minutes, followed by filtration. The solid thus obtained is washed with water followed by ethanol, and dried under reduced pressure-[0058] This process provides (1-(9-[(4S,2R,3R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-6-aminopurin-2-yl) pyrazol-4-yl)-N-methylcarboxamide as its monohydrate. This polymorph can be further purified by dissolving in dimethylsulfoxide, filtering any solid impurities from the solution, and precipitating the monohydrate from solution by addition of water.
EXAMPLE I
Preparation of Ethyl-2-formyl-3-oxopropionate CO2Et H H YI- O O
10059] A three- or four-neck round bottom flask equipped with magnetic stir bar, thermocouple, digital thermometer, gas inlet and outlet and addition funnel was flushed with argon- Ethyl 3,3-diethoxypropionate (64.5 g) in tetrahydrofuran were charged to the addition funnel. Sodium hydride (21.2 g of a 60% dispersion) was charged to the reaction flask followed by tetrahydrofuran. The contents of the flask were cooled to 0-C in an ice-bath, and ethyl formate (257 g) was added. The mixture was cooled to 0-5 C and the contents of the addition funnel added dropwise, maintaining an internal temperature of less than 5 C. The ice-bath was removed and the contents allowed to warm to ambient temperature. Consumption of ethyl 3,3-diethoxypropionate was monitored by TLC analysis. The reaction was quenched by addition of ice-water (10.6 vol), and extracted three times with methyl t-butyl ether (5.4 vol each), and the organic layers discarded. The aqueous phase was acidified with conc. hydrochloric acid to a pH of 1 to 1.5. The acidified aqueous layer was extracted three times with dichloromethane and the combined organic layers dried over sodium sulfate. The solvent was removed under reduced pressure, and the residue distilled under vacuum, to provide ethyl 2-formyl-3-oxopropionate, 27.92 g, 70% yield.
A. Preparation of 2-Hydrazinoadenosine (2) N
N
HzNHNN N
O
(2) HO
[0060] A flask equipped with a mechanical stirrer, gas inlet, gas outlet and thermocouple was flushed with argon. 2-Chloroadenosine hemihydrate (53.1 g) was added, followed by hydrazine monohydrate (134 g). The mixture was stirred while heating to 40-45 C for 2 hours. The progress of the reaction was followed by TLC
analysis. When the reaction was complete, the heat source was removed and ethanol (800ml) was added. The mixture was stirred for 2 hours at ambient temperature, then the precipitate collected by filtration. The filter cake was washed with ethanol and dried under reduced pressure for 30 minutes. The solids were transferred to a clean flask equipped with a mechanical stirrer and water (300 ml) was added. The suspension was stirred at room temperature for 18 hours, and the solids isolated by filtration. The filter cake was washed with ice-cold water (300 ml) followed by a wash with ice-cold ethanol (300 ml). The solid was dried under reduced pressure to provide 2-hydrazinoadenosine (41.38g, 81.4% yield, 99.3% purity).
B. Alternative Preparation of 2-Hydrazinoadenosine (2) [0061] A reaction vessel containing hydrazine hydrate (258 g, 250 ml) was heated to 40-50 C. To the warm mixture 2-chloroadenosine hemihydrate (100 g) was added in portions, maintaining the temperature between 45-55 C. The temperature was kept at this temperature for two hours, and then deionized water (500 ml) was added over a period of 30 minutes, maintaining the temperature at 45-55 C. The mixture was then gradually cooled to 0-5 C over a period of 3 hours, then stirred at this temperature for a further 30 minutes. The solid was then filtered off, and washed with cold (2-5 C) deionized water (200 ml), followed by ethanol (400 ml). The solid was dried under vacuum for 12 hours, to provide 2-hydrazinoadenosine.
Preparation of Ethyl 1-f 9-[(4S,2R,3R,5R -3,4-dihy roxy-5-(hydroxymethyl)oxolan-2-yll-6-aminopurin-2-yl)pyrazole-4-carboxylate (3) N N
O jl N
'\OH
t3, HO
[0062] Ethyl 2-formyl-3-oxopropionate (23.93g, 0.17 mol) was placed in a flask equipped with mechanical stirrer, gas inlet, gas outlet and reflux condenser.
2-Propanol was added to the flask followed by 2-hydrazinoadenosine (44.45g, 0.15 mol).
The mixture was heated to reflux under stirring for 2-4 hours, following the progress of the reaction by TLC analysis. When the reaction was judged complete, the heat source was removed and the mixture cooled to room temperature. The suspension was cooled under stirring in an ice-bath for 1.5 to 2 hours. The solids were isolated by vacuum filtration, and washed with ice-cold 2-propanol. The product, ethyl I- {9-[(4S,2R,3 R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-6-aminopurin-2-yl}pyrazole-4-carboxylate, was dried under reduced pressure to a constant weight.
Yield 54.29g, purity (by HPLC) 96.6%.
Preparation of (1- {9-[(4S,2R.3R,5R)-3,4-dihydroxy-5-(hhydroxymeth )oxolan-2-yll-6-aminopurin-2-yl) Uyrazol-4-yl)-N-methylcarboxamide N
o N I ~ ~.
~N
~~OH
HO
[0063] A mixture of ethyl 1-{9-[(4S,2R,3R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-6-aminopurin-2-yl)pyrazole-4-carboxylate (46.4 g) and methylamine (40% in water, 600 ml) was stirred at ambient temperature for about 4 hours, following the progress of the reaction by HPLC analysis. The majority of the excess methylamine was removed under reduced pressure, and the remaining mixture cooled at 0 C for 2 hours. The solid material was filtered off, washed with ice-cold 200 proof ethanol, and dried under reduced pressure, to provide (1-{9-[(4S,2R,3R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-6-aminopurin-2=y1 } pyrazol-4-yl)-N-methylcarboxamide as its monohydrate, 36.6 g, purity 99.6%_ [0064] The structure of the material was confirmed by 'H NMR (see Figure 1 and below). Thermal analysis (see Figure 2) provided results consistent with the presence of one molecule of water. X-Ray powder diffraction patterns were obtained (Figure 3) 4a N
N
I ~ 6 O a N 7. C/A r H3C- ~N
e C 0 a 3"OH
HO 5.
'H and 13C NMR spectra were obtained in the following manner. Two samples of the material obtained above were weighed out and dissolved in d6-DMSO - 5.3 mg was used for the 'H spectra, and 20.8 mg was used for 13C spectra- All spectra were TM
acquired at ambient temperature on a JEOL Eclipse 400 spectrometer operating at 400 MHz for 'H and 100 MHz for 13C.
Label C shift(ppm) 'H shift(ppm). Multiplicity, splitting(Hz) 2 150.5 or 150.3 -4 156.4 -4a 117.9 -6 140.0 8.41 s 7a 150.5 or 150.3 -1' 86.9 5.94 D, 6.2 2' 73.7 4.62 m 2'-OH - 5.50 D, 6.2 3' 70.5 4.17 m 3'-OH - 5.23 D, 4.7 4' 85.7 3.96 m 5' 61.5 3.67, 3.57 m 5'-OH - 5.02 D, 5.7 A 140.9 8.07 D, 0.8 B 120.2 -C 129.6 8.95 D, 0.8 D 161.7 -E 25.6 2.76 D, 4.6 NH2 - 7.77 brs NH - 8.35 Q, 4.6 Purification of (1-{9-[(4S,2R,3R,5R) 3,4-dihydrox -55-(hydroxymethyl)oxolan-2-y1]-6-aminopurin-2-yl) pyrazol-4-yl)-N-methylcarboxamide monohydrate [0065] A solution of (1- {9-[(4S,2R,3R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-6-aminopurin-2-yl) pyrazol-4-yl)-N-methylcarboxamide monohydrate (100 g) in dimethylsulfoxide (300 ml) was filtered through a 0.6 to 0.8 micron prefilter and a 0.2 micron filter to remove any solid impurities. The filtrate was then slowly added over a period of 1 hour to deionized water (1 liter) with stirring, and the slurry thus produced stirred for not less than 1 hour. The solid was filtered off, washed with deionized water (2 x 1 liter), and dried under vacuum for not less than 1 hour- The dried product was then slurried again with deionized water (1.5 liter) for not less than 2 hours, filtered off, and washed with deionized water (1 liter) followed by absolute ethanol (750 ml). The purified product was dried under vacuum at a temperature of not more than 40 C for not less than 12 hours, to provide (1-{9-[(4S,2R,3R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-6-aminopurin-2-yl}pyrazol-4-yl)-N-methylcarboxamide monohydrate free of any 2-hydrazinoadenosine impurity.
Preparation of Ethyl (2E)-3-({9-((4S,2R,3R,5R)-3,4-dihydroxy-5-(hydrox ny_ethyl)-oxolan-2-yl]-2-[4-(ethoxycarbonyl)p yllpurin-6-yl}amino)-2-forrnylprop-2-enoate H
0 0 el I
H
N N
NN
~~OH
[0066] A mixture of 2-hydrazinoadenosine (100 g, 0.34 mol), ethyl 2-formyl-3-oxopropionate (242g, 1.7 mol) and absolute ethanol were charged to a reactor, and the mixture heated to reflux for 2 hours. When the reaction was judged complete, the heat source was removed and the mixture gradually cooled to 5-10 C over a period of hours. The slurry was stirred for 30 minutes at this temperature, and the mixture filtered. The solid material was washed with cold (5-10 C) absolute ethanol, and then dried under vacuum at a temperature that did not exceed 40 C, to provide ethyl (2E)-3-({9-[(4S,2R,3 R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl] -2-[4-(ethoxycarbonyl)-pyrazolyljpurin-6--yl} amino)-2-formylprop-.2-enoate.
[00671 An elemental analysis gave the following results: C, 48.75%; H, 4.86%;
N, 18.05%; 0, 27.57. Theoretical: C, 49.72%; H, 4.74%; N, 18.45%; 0, 27.09. The analysis corresponds within experimental error limits to the hemihydrate of the desired product. (C, 48.89%; H, 4.81%; N, 18.1%; O, 28.12) [00681 1H and 13C NMR spectra were obtained in the following manner. 20.2mg of the compound of formula (4) was dissolved in -x0.75 nil of DMSO-d6, and the spectra TM
obtained at ambient temperature on a JEOL ECX-400 N R spectrometer operating at 400 MHz for 1H and 100 MHz for 13C. The chemical shifts were referenced to the DMSO solvent, 2.50 ppm for 'H and 39.5 ppm for 13C.
RESULTS
[00691 The 'H and 13C chemical shifts are listed in Table 1. Two isomers in a ratio of---60/30 were observed in both the 'H and the 13C spectra, labeled as major and minor in the table.
Atoms C Chemical H Chemical Multiplicit , Shift (ppm) Shift (pp! La) (Hz) 21(major) - 192.4 9.96 d, 3.6 21 minor 187.6 9.83 S
22 minor) 167.1 - -22(major) 165.2 - -15(minor 161.8 = - -15(major) 161.7 - -6(major) 153.1 - -6minor 152.9 - -2(minor) 149.4 - -2(major) 149.3 - -19(minor) 148.0 9.22 d, 13.0 4(minor) 147.9 - -4(major) 147.8 - -19(major) 147.51 .9.26 d, 12.4, d, 3.6 8(major) 144.9 8.87 S
8(minor) 144.7 8.85 s 12 143.1 8.20-8.23 m 14(minor) 132.8 9.20 d, -0.7 14(major) 132.6 9.12 d,-0.7 5(major) 120.7 - -5(minor 120.6 - -13 116.7 - -20(minor) 107.2 - -20(major) 106.1 - -1 ' (major) 87.9 6.07 d, 5.3 1 ' (minor) 87.9- 6.06 d, 5.3 4' 85.8 4.02 g, 3.9 2' minor) 74.1 4.62 g, -5.4 2'(major) 74.1 4.61 g, --5.4 3' 70.1 4.22 g,4.2 5' 61.0 3.62, 3.73 m 23, 16 60.3-60.8 4.25-4.39 m 17,24 14.1-14.2 1.28-1.38 m 18(major) - 12.51 d, 12.4 18(minor) - 11.47 d, 13.0 2'-OH (major) - 5.63 d, 6.1 2'-OH (minor) - 5.62 d, 6.1 3'-OH - 5.30 d, 5.1 5'-OH - 5.08 t, 5.5 The compound of formula (4) was confirmed to be a mixture of the following two isomers:
O
\8 N H 19 2Y20 H
N N 3 ~N 5 <N3 8 2 ~N11 8 H._-\I , 2 11 4~ 1 6 N 10 N H 12 5' 4. 7 N 6 1~ N 10 N N H12 HO n,.H H 13 HO 1õ H H
HO OH2 0 15 \-- CH 3' HO OH2= 0 15 Major Minor Preparation of (1-{9-[(4S,2R,3R,5R)-3,4-dihydroxy-5-(hymethyl)oxolan-2-yl]-6-aminopurin-2-yllpyrazol-4-yl -N-methylcarboxamide from Compound (4) NHZ
N
N
"OH
""-NH ~f N
'OH
HO
[00701 Aqueous 40% methylamine solution (1300 ml) was placed in a pressure reactor, cooled to 0-5 C, and the product of Example 5 (ethyl (2E)-3-((9-[(4S,2R,3R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-2-[4-(ethoxycarbonyl)pyrazolyl]purin-6-yl}amino)-2-formylprop-2-enoate (100 g) added.
The mixture was stirred at 0-5 C for at least 8 hours, monitoring the reaction for completion. When complete, the mixture was warmed, maintaining the temperature between 50 and 60 C for 1 hour, and then cooled to less than 30 C over a period of 1 hour. When the temperature was below 30 C, the mixture was degassed using a pressure of 100-150 mm Hg, allowing the temperature to decrease to 0-5 C. The mixture was stirred at 0-5 C for at least 1 hour, maintaining the pressure at mm Hg. The vacuum was then discontinued and replaced by nitrogen, maintaining the temperature at 0-5 C for not less than 30 minutes. The solid product was then filtered off, washed with water (3 x 500 ml), then with absolute ethanol (625 ml). The product was dried under vacuum, not allowing the temperature to exceed 40 C, to provide (1-{9-[(4S,2R, 3R,5R)-3,4-dihydroxy-5 -(hydroxymethyl)oxolan-2-yl] -6-aminopurin-yl) pyrazol-4-yl)-N-methylcarboxamide as its monohydrate.
[0071j 1H and 13C NMR spectra were obtained in the following manner. Two samples of the material obtained above were weighed out and dissolved in d6-DMSO.-5.3 mg was used for the 'H spectra, and 20.8 mg was used for 13C spectra. All spectra TM
were acquired at ambient temperature on a JEOL Eclipse'' 400 spectrometer operating at 400 MHz for 'H and 100 MHz for 13C_ Label C shift(ppm) 'H shift(ppm) Multiplicity, splitting(Hz) 2 150.5 or 150.3 -4 156.4 -4a 117.9 -6 140.0 8.41 s 7a 150.5 or 150.3 -1' 86.9 5.94 D, 6.2 2' 73.7 4.62 m 2'-OH - 5.50 D, 6.2 3' 70.5 4.17 m 3'-OH - 5.23 D, 4.7 .41 85.7 3.96 m 5' 61.5 3.67, 3.57 m 5'-OH - - 5.02 D, 5.7 A 140.9 8.07 D, 0.8 B 120.2 -C 129.6 8.95 D, 0.8 D 161.7 -E 25.6 2.76 D, 4.6 NH2 - 7.77 brs NH - 8.35 Q, 4.6 [00721 An elemental analysis gave the following results: C, 43.96%; H, 4.94%;
N, 27.94. Theoretical: C, 44.12%; H, 4.94%; N, 27.44%; 0, 27.09. The analysis corresponds within experimental error limits to the monohydrate.
REACTION SCHEME I
C02Et HY
H
111111011 where Et is ethyl 0 (2) (l) "'OH "'OH
O
J-11, N ~'~
Eto ~'N
-N
(3) H
H
Step 1 - Preparation of Formula (2) [0046] The compound of formula (2) is prepared from the compound of formula (1) by reaction with hydrazine monohydrate in the absence of a solvent. The reaction is conducted at a temperature of about 40 C plus/minus 5 C. When the reaction is complete, the product of formula (2) is isolated by stirring with a protic solvent in which the compound of formula (2) has limited solubility, for example ethanol or isopropanol. The mixture is stirred for about 1-5 hours, and then filtered.
The solid is purified by stirring with water, filtering, and washing with water followed by isopropanol and dried under vacuum, which is taken to the next step without purification.
Step 2 - Preparation of Formula (3) [0047] The compound of formula (2) is then converted to a compound of formula (3) by reacting with about 1-1.2 molar equivalents of ethyl 2-formyl-3-oxopropionate.
The reaction is conducted in a protic solvent, preferably ethanol, at about reflux temperature, for about 2-4 hours. After cooling, to about 0 C, the solid is filtered off, washed with cold ethanol, and dried under reduced pressure. The product of formula (3) is taken to the next step without purification.
Step 3 - Preparation of Final Product [0048] The final product is prepared from the compound of formula (3) by reacting with methylamine, preferably aqueous methylamine. The reaction is carried out at about room temperature, for about 4 hours. The product of Formula I is isolated by conventional means, for example by filtration, washing the solid with cold ethanol, and drying under reduced pressure.
Preparation of Starting Materials [0049] (4S,2R,3R,5R)-2-(6-amino-2-chloropurin-9-yl)-5-(hydroxymethyl)oxolane-3,4-diol is used as a starting material in step 1. This compound is commercially available.
[0050] Ethyl 2-formyl-3-oxopropanoate is used as a starting material in step 2. It is commercially available, or may be made as shown in Reaction Scheme 11.
REACTION SCHEME II
OR
a NaH
H OEt EtO OEt COZEt where Et is ethyl [0051] Ethyl 3,3-diethoxypropionate is reacted with ethyl formate in the presence of a strong base, preferably sodium hydride. The reaction is carried out at about 0-5 C, for about 24 hours. The product is isolated by conventional means, for example by the addition of water and extraction of impurities with a conventional solvent, for example t-butyhnethyl ether, acidification of the aqueous phase with, for example, hydrochloric acid, followed by extraction with a solvent such as dichloromethane, and removing the solvent from the dried extract under reduced pressure-[00521 A preferred method for the large scale synthesis of (1-{9-[(4S,2R,3R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-6-aminopurin-2-yl} pyrazol-4-yl)-N-methylcarboxamide is shown in Reaction Scheme III.
REACTION SCHEME III
NHZ NHZ
N Et I \ I \ H(H
Cl N N HZNHN N N
H
where Et is ethyl (~) (2) 'OH
H H
NHZ
H =
NCH N CH3NH2 \/\
O
A `\ N N I "No"
N N -NH N
l^-d N
OH
(4) Ho-HO-Step 1 - Preparation of Formula (2) [0053) The compound of formula (2) is prepared from the compound of formula (1) by reaction with hydrazine monohydrate in the absence of a solvent. The reaction is conducted at a temperature of about 45-55 C plus/minus 5 C. When the reaction is complete, the product of formula (2) is isolated by stirring with a protic solvent in which the compound of formula (2) has limited solubility, for example ethanol or isopropanol. The mixture is stirred for about 1-5 hours, and then filtered.
The solid is purified by stirring with water, filtering, and washing with water followed by ethanol or isopropanol and dried under vacuum, which is taken to the next step without purification.
Step 2 - Preparation of Formula (4) 100541 The compound of formula (2) is then converted to a compound of formula (4) by reacting with an excess of ethyl 2-formyl-3-oxopropionate, for example a 2-10 fold excess, preferably about 5-10 fold excess. The reaction is conducted in a protic solvent, for example ethanol, at about reflux temperature, for about 2-4 hours. After cooling, to about 0 C, the solid is filtered off, washed with cold ethanol, and dried under reduced pressure, and the product of formula (4) is taken to the next step without purification.
[0055] The compound of formula (4) is drawn as a (2E) alkene derivative, as this is the major isomer formed in this reaction. However, it should be noted that a significant amount of the (2Z) alkene derivative may also be formed in this reaction; that is:
H O/ \
H NH
N N
O
N
i N
``-NOH
~O /N
~~OH
HO
named as ethyl (2Z)-3-({ 9-[(4S,2R,3R,5R)-3,4-dihydroxy-5-(hydroxymethyl)-oxolan-2-yl]-2-[4-(ethoxycarbonyl)pyrazolyljpurin-6-yl} amino)-2-formylprop-2-enoate.
[0056] Accordingly, although the compound of formula (4) is represented as the (2E) alkene derivative only, the term "compound of formula (4)" is intended to include both the instance where it is solely the (2E) isomer, and the instance where the major portion of the product is the (2E) isomer and a minor portion of the (2Z) isomer is also present. The conversion of the compound of formula (4) to the final product by reaction with methylamine as described in Step 3 proceeds in the same manner whether the compound of formula (4) is present as the (2E) isomer or as a mixture of the (2E) isomer and the (2Z) isomer.
Step 3 - Preparation of Final Product 10057] The final product is prepared from the compound of formula (4) by reacting with methylamine, preferably aqueous methylamine. The reaction is initially carried out at about 0-5 C for about 8 hours, preferably in a pressure reactor, followed by raising the temperature to 50-60 C over about 1 hour, and maintaining the temperature for 15-30 minutes. The product is isolated by conventional means, for example by cooling to 0-5 C and maintaining a vacuum for about 1 hour, thus removing the methylamine. The vacuum is removed, and the remaining contents held at 0-5 C
for at least 30 minutes, followed by filtration. The solid thus obtained is washed with water followed by ethanol, and dried under reduced pressure-[0058] This process provides (1-(9-[(4S,2R,3R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-6-aminopurin-2-yl) pyrazol-4-yl)-N-methylcarboxamide as its monohydrate. This polymorph can be further purified by dissolving in dimethylsulfoxide, filtering any solid impurities from the solution, and precipitating the monohydrate from solution by addition of water.
EXAMPLE I
Preparation of Ethyl-2-formyl-3-oxopropionate CO2Et H H YI- O O
10059] A three- or four-neck round bottom flask equipped with magnetic stir bar, thermocouple, digital thermometer, gas inlet and outlet and addition funnel was flushed with argon- Ethyl 3,3-diethoxypropionate (64.5 g) in tetrahydrofuran were charged to the addition funnel. Sodium hydride (21.2 g of a 60% dispersion) was charged to the reaction flask followed by tetrahydrofuran. The contents of the flask were cooled to 0-C in an ice-bath, and ethyl formate (257 g) was added. The mixture was cooled to 0-5 C and the contents of the addition funnel added dropwise, maintaining an internal temperature of less than 5 C. The ice-bath was removed and the contents allowed to warm to ambient temperature. Consumption of ethyl 3,3-diethoxypropionate was monitored by TLC analysis. The reaction was quenched by addition of ice-water (10.6 vol), and extracted three times with methyl t-butyl ether (5.4 vol each), and the organic layers discarded. The aqueous phase was acidified with conc. hydrochloric acid to a pH of 1 to 1.5. The acidified aqueous layer was extracted three times with dichloromethane and the combined organic layers dried over sodium sulfate. The solvent was removed under reduced pressure, and the residue distilled under vacuum, to provide ethyl 2-formyl-3-oxopropionate, 27.92 g, 70% yield.
A. Preparation of 2-Hydrazinoadenosine (2) N
N
HzNHNN N
O
(2) HO
[0060] A flask equipped with a mechanical stirrer, gas inlet, gas outlet and thermocouple was flushed with argon. 2-Chloroadenosine hemihydrate (53.1 g) was added, followed by hydrazine monohydrate (134 g). The mixture was stirred while heating to 40-45 C for 2 hours. The progress of the reaction was followed by TLC
analysis. When the reaction was complete, the heat source was removed and ethanol (800ml) was added. The mixture was stirred for 2 hours at ambient temperature, then the precipitate collected by filtration. The filter cake was washed with ethanol and dried under reduced pressure for 30 minutes. The solids were transferred to a clean flask equipped with a mechanical stirrer and water (300 ml) was added. The suspension was stirred at room temperature for 18 hours, and the solids isolated by filtration. The filter cake was washed with ice-cold water (300 ml) followed by a wash with ice-cold ethanol (300 ml). The solid was dried under reduced pressure to provide 2-hydrazinoadenosine (41.38g, 81.4% yield, 99.3% purity).
B. Alternative Preparation of 2-Hydrazinoadenosine (2) [0061] A reaction vessel containing hydrazine hydrate (258 g, 250 ml) was heated to 40-50 C. To the warm mixture 2-chloroadenosine hemihydrate (100 g) was added in portions, maintaining the temperature between 45-55 C. The temperature was kept at this temperature for two hours, and then deionized water (500 ml) was added over a period of 30 minutes, maintaining the temperature at 45-55 C. The mixture was then gradually cooled to 0-5 C over a period of 3 hours, then stirred at this temperature for a further 30 minutes. The solid was then filtered off, and washed with cold (2-5 C) deionized water (200 ml), followed by ethanol (400 ml). The solid was dried under vacuum for 12 hours, to provide 2-hydrazinoadenosine.
Preparation of Ethyl 1-f 9-[(4S,2R,3R,5R -3,4-dihy roxy-5-(hydroxymethyl)oxolan-2-yll-6-aminopurin-2-yl)pyrazole-4-carboxylate (3) N N
O jl N
'\OH
t3, HO
[0062] Ethyl 2-formyl-3-oxopropionate (23.93g, 0.17 mol) was placed in a flask equipped with mechanical stirrer, gas inlet, gas outlet and reflux condenser.
2-Propanol was added to the flask followed by 2-hydrazinoadenosine (44.45g, 0.15 mol).
The mixture was heated to reflux under stirring for 2-4 hours, following the progress of the reaction by TLC analysis. When the reaction was judged complete, the heat source was removed and the mixture cooled to room temperature. The suspension was cooled under stirring in an ice-bath for 1.5 to 2 hours. The solids were isolated by vacuum filtration, and washed with ice-cold 2-propanol. The product, ethyl I- {9-[(4S,2R,3 R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-6-aminopurin-2-yl}pyrazole-4-carboxylate, was dried under reduced pressure to a constant weight.
Yield 54.29g, purity (by HPLC) 96.6%.
Preparation of (1- {9-[(4S,2R.3R,5R)-3,4-dihydroxy-5-(hhydroxymeth )oxolan-2-yll-6-aminopurin-2-yl) Uyrazol-4-yl)-N-methylcarboxamide N
o N I ~ ~.
~N
~~OH
HO
[0063] A mixture of ethyl 1-{9-[(4S,2R,3R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-6-aminopurin-2-yl)pyrazole-4-carboxylate (46.4 g) and methylamine (40% in water, 600 ml) was stirred at ambient temperature for about 4 hours, following the progress of the reaction by HPLC analysis. The majority of the excess methylamine was removed under reduced pressure, and the remaining mixture cooled at 0 C for 2 hours. The solid material was filtered off, washed with ice-cold 200 proof ethanol, and dried under reduced pressure, to provide (1-{9-[(4S,2R,3R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-6-aminopurin-2=y1 } pyrazol-4-yl)-N-methylcarboxamide as its monohydrate, 36.6 g, purity 99.6%_ [0064] The structure of the material was confirmed by 'H NMR (see Figure 1 and below). Thermal analysis (see Figure 2) provided results consistent with the presence of one molecule of water. X-Ray powder diffraction patterns were obtained (Figure 3) 4a N
N
I ~ 6 O a N 7. C/A r H3C- ~N
e C 0 a 3"OH
HO 5.
'H and 13C NMR spectra were obtained in the following manner. Two samples of the material obtained above were weighed out and dissolved in d6-DMSO - 5.3 mg was used for the 'H spectra, and 20.8 mg was used for 13C spectra- All spectra were TM
acquired at ambient temperature on a JEOL Eclipse 400 spectrometer operating at 400 MHz for 'H and 100 MHz for 13C.
Label C shift(ppm) 'H shift(ppm). Multiplicity, splitting(Hz) 2 150.5 or 150.3 -4 156.4 -4a 117.9 -6 140.0 8.41 s 7a 150.5 or 150.3 -1' 86.9 5.94 D, 6.2 2' 73.7 4.62 m 2'-OH - 5.50 D, 6.2 3' 70.5 4.17 m 3'-OH - 5.23 D, 4.7 4' 85.7 3.96 m 5' 61.5 3.67, 3.57 m 5'-OH - 5.02 D, 5.7 A 140.9 8.07 D, 0.8 B 120.2 -C 129.6 8.95 D, 0.8 D 161.7 -E 25.6 2.76 D, 4.6 NH2 - 7.77 brs NH - 8.35 Q, 4.6 Purification of (1-{9-[(4S,2R,3R,5R) 3,4-dihydrox -55-(hydroxymethyl)oxolan-2-y1]-6-aminopurin-2-yl) pyrazol-4-yl)-N-methylcarboxamide monohydrate [0065] A solution of (1- {9-[(4S,2R,3R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-6-aminopurin-2-yl) pyrazol-4-yl)-N-methylcarboxamide monohydrate (100 g) in dimethylsulfoxide (300 ml) was filtered through a 0.6 to 0.8 micron prefilter and a 0.2 micron filter to remove any solid impurities. The filtrate was then slowly added over a period of 1 hour to deionized water (1 liter) with stirring, and the slurry thus produced stirred for not less than 1 hour. The solid was filtered off, washed with deionized water (2 x 1 liter), and dried under vacuum for not less than 1 hour- The dried product was then slurried again with deionized water (1.5 liter) for not less than 2 hours, filtered off, and washed with deionized water (1 liter) followed by absolute ethanol (750 ml). The purified product was dried under vacuum at a temperature of not more than 40 C for not less than 12 hours, to provide (1-{9-[(4S,2R,3R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-6-aminopurin-2-yl}pyrazol-4-yl)-N-methylcarboxamide monohydrate free of any 2-hydrazinoadenosine impurity.
Preparation of Ethyl (2E)-3-({9-((4S,2R,3R,5R)-3,4-dihydroxy-5-(hydrox ny_ethyl)-oxolan-2-yl]-2-[4-(ethoxycarbonyl)p yllpurin-6-yl}amino)-2-forrnylprop-2-enoate H
0 0 el I
H
N N
NN
~~OH
[0066] A mixture of 2-hydrazinoadenosine (100 g, 0.34 mol), ethyl 2-formyl-3-oxopropionate (242g, 1.7 mol) and absolute ethanol were charged to a reactor, and the mixture heated to reflux for 2 hours. When the reaction was judged complete, the heat source was removed and the mixture gradually cooled to 5-10 C over a period of hours. The slurry was stirred for 30 minutes at this temperature, and the mixture filtered. The solid material was washed with cold (5-10 C) absolute ethanol, and then dried under vacuum at a temperature that did not exceed 40 C, to provide ethyl (2E)-3-({9-[(4S,2R,3 R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl] -2-[4-(ethoxycarbonyl)-pyrazolyljpurin-6--yl} amino)-2-formylprop-.2-enoate.
[00671 An elemental analysis gave the following results: C, 48.75%; H, 4.86%;
N, 18.05%; 0, 27.57. Theoretical: C, 49.72%; H, 4.74%; N, 18.45%; 0, 27.09. The analysis corresponds within experimental error limits to the hemihydrate of the desired product. (C, 48.89%; H, 4.81%; N, 18.1%; O, 28.12) [00681 1H and 13C NMR spectra were obtained in the following manner. 20.2mg of the compound of formula (4) was dissolved in -x0.75 nil of DMSO-d6, and the spectra TM
obtained at ambient temperature on a JEOL ECX-400 N R spectrometer operating at 400 MHz for 1H and 100 MHz for 13C. The chemical shifts were referenced to the DMSO solvent, 2.50 ppm for 'H and 39.5 ppm for 13C.
RESULTS
[00691 The 'H and 13C chemical shifts are listed in Table 1. Two isomers in a ratio of---60/30 were observed in both the 'H and the 13C spectra, labeled as major and minor in the table.
Atoms C Chemical H Chemical Multiplicit , Shift (ppm) Shift (pp! La) (Hz) 21(major) - 192.4 9.96 d, 3.6 21 minor 187.6 9.83 S
22 minor) 167.1 - -22(major) 165.2 - -15(minor 161.8 = - -15(major) 161.7 - -6(major) 153.1 - -6minor 152.9 - -2(minor) 149.4 - -2(major) 149.3 - -19(minor) 148.0 9.22 d, 13.0 4(minor) 147.9 - -4(major) 147.8 - -19(major) 147.51 .9.26 d, 12.4, d, 3.6 8(major) 144.9 8.87 S
8(minor) 144.7 8.85 s 12 143.1 8.20-8.23 m 14(minor) 132.8 9.20 d, -0.7 14(major) 132.6 9.12 d,-0.7 5(major) 120.7 - -5(minor 120.6 - -13 116.7 - -20(minor) 107.2 - -20(major) 106.1 - -1 ' (major) 87.9 6.07 d, 5.3 1 ' (minor) 87.9- 6.06 d, 5.3 4' 85.8 4.02 g, 3.9 2' minor) 74.1 4.62 g, -5.4 2'(major) 74.1 4.61 g, --5.4 3' 70.1 4.22 g,4.2 5' 61.0 3.62, 3.73 m 23, 16 60.3-60.8 4.25-4.39 m 17,24 14.1-14.2 1.28-1.38 m 18(major) - 12.51 d, 12.4 18(minor) - 11.47 d, 13.0 2'-OH (major) - 5.63 d, 6.1 2'-OH (minor) - 5.62 d, 6.1 3'-OH - 5.30 d, 5.1 5'-OH - 5.08 t, 5.5 The compound of formula (4) was confirmed to be a mixture of the following two isomers:
O
\8 N H 19 2Y20 H
N N 3 ~N 5 <N3 8 2 ~N11 8 H._-\I , 2 11 4~ 1 6 N 10 N H 12 5' 4. 7 N 6 1~ N 10 N N H12 HO n,.H H 13 HO 1õ H H
HO OH2 0 15 \-- CH 3' HO OH2= 0 15 Major Minor Preparation of (1-{9-[(4S,2R,3R,5R)-3,4-dihydroxy-5-(hymethyl)oxolan-2-yl]-6-aminopurin-2-yllpyrazol-4-yl -N-methylcarboxamide from Compound (4) NHZ
N
N
"OH
""-NH ~f N
'OH
HO
[00701 Aqueous 40% methylamine solution (1300 ml) was placed in a pressure reactor, cooled to 0-5 C, and the product of Example 5 (ethyl (2E)-3-((9-[(4S,2R,3R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-2-[4-(ethoxycarbonyl)pyrazolyl]purin-6-yl}amino)-2-formylprop-2-enoate (100 g) added.
The mixture was stirred at 0-5 C for at least 8 hours, monitoring the reaction for completion. When complete, the mixture was warmed, maintaining the temperature between 50 and 60 C for 1 hour, and then cooled to less than 30 C over a period of 1 hour. When the temperature was below 30 C, the mixture was degassed using a pressure of 100-150 mm Hg, allowing the temperature to decrease to 0-5 C. The mixture was stirred at 0-5 C for at least 1 hour, maintaining the pressure at mm Hg. The vacuum was then discontinued and replaced by nitrogen, maintaining the temperature at 0-5 C for not less than 30 minutes. The solid product was then filtered off, washed with water (3 x 500 ml), then with absolute ethanol (625 ml). The product was dried under vacuum, not allowing the temperature to exceed 40 C, to provide (1-{9-[(4S,2R, 3R,5R)-3,4-dihydroxy-5 -(hydroxymethyl)oxolan-2-yl] -6-aminopurin-yl) pyrazol-4-yl)-N-methylcarboxamide as its monohydrate.
[0071j 1H and 13C NMR spectra were obtained in the following manner. Two samples of the material obtained above were weighed out and dissolved in d6-DMSO.-5.3 mg was used for the 'H spectra, and 20.8 mg was used for 13C spectra. All spectra TM
were acquired at ambient temperature on a JEOL Eclipse'' 400 spectrometer operating at 400 MHz for 'H and 100 MHz for 13C_ Label C shift(ppm) 'H shift(ppm) Multiplicity, splitting(Hz) 2 150.5 or 150.3 -4 156.4 -4a 117.9 -6 140.0 8.41 s 7a 150.5 or 150.3 -1' 86.9 5.94 D, 6.2 2' 73.7 4.62 m 2'-OH - 5.50 D, 6.2 3' 70.5 4.17 m 3'-OH - 5.23 D, 4.7 .41 85.7 3.96 m 5' 61.5 3.67, 3.57 m 5'-OH - - 5.02 D, 5.7 A 140.9 8.07 D, 0.8 B 120.2 -C 129.6 8.95 D, 0.8 D 161.7 -E 25.6 2.76 D, 4.6 NH2 - 7.77 brs NH - 8.35 Q, 4.6 [00721 An elemental analysis gave the following results: C, 43.96%; H, 4.94%;
N, 27.94. Theoretical: C, 44.12%; H, 4.94%; N, 27.44%; 0, 27.09. The analysis corresponds within experimental error limits to the monohydrate.
Claims (3)
PRIVILEGE IS CLAIMED ARE DEFINED AS FOLLOWS:
1. A method of synthesizing (1- {9-[(4S,2R,3R,5R)-3,4-dihydroxy-5(hydroxymethyl)oxolan-2-yl]-6-aminopurin-2-yl}pyrazol-4-yl)-N-methylcarboxamide comprising contacting a compound of the formula (4):
with an aqueous solution of methylamine at an initial temperature of about 0-5°C followed by warming to about 50-70°C in a sealed pressure reactor.
with an aqueous solution of methylamine at an initial temperature of about 0-5°C followed by warming to about 50-70°C in a sealed pressure reactor.
2. The method of claim 1 further comprising wherein the (1-{9-[(4S,2R,3R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-6-aminopurin-2-yl}pyrazol-4-yl)-N-methylcarboxamide end product is isolated as the pure monohydrate by (a) dissolving the product of claim 1 in a solvent, (b) adding purified water, (c) filtering the slurry thus formed, (d) washing the contents of the filter with water followed by ethanol, and (e) drying the solid that remains under vacuum at a temperature that does not exceed 40°C.
3. The method of claim 2, wherein the solvent used in step (a) is dimethylsulfoxide.
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