CA2743477A1 - Instruments and method for exposing a receptacle to multiple thermal zones - Google Patents
Instruments and method for exposing a receptacle to multiple thermal zones Download PDFInfo
- Publication number
- CA2743477A1 CA2743477A1 CA2743477A CA2743477A CA2743477A1 CA 2743477 A1 CA2743477 A1 CA 2743477A1 CA 2743477 A CA2743477 A CA 2743477A CA 2743477 A CA2743477 A CA 2743477A CA 2743477 A1 CA2743477 A1 CA 2743477A1
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- Prior art keywords
- thermal
- instrument
- receptacle
- multiple chamber
- chambers
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medical Preparation Storing Or Oral Administration Devices (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Control Of Temperature (AREA)
Abstract
An instrument for processing a sample in a receptacle having a plurality of interconnected chambers is provided. The instrument has thermal elements defining a multiple chamber thermal zone in thermal communication with the receptacle and which to transmit thermal energy between each multiple chamber thermal zone and an associated region of the receptacle. The associated region encompasses all or a portion of each of two or more but less than all chambers. A controller is programmed to control operation of the thermal elements to heat or cool the chambers. A method for heating or cooling substances within the receptacle is also provided where the receptacle is positioned in thermal communication with chamber thermal zones contained in an analyzer and thermal energy is transmitted between each multiple chamber thermal zone and the chambers to heat or cool substances contained within the chambers to a temperature different than the temperature of other chambers.
Claims (42)
1. An instrument for processing a sample in a receptacle having a plurality of interconnected chambers, the instrument being constructed and arranged to support the receptacle in an operative position during the processing and comprising:
one or more thermal elements each defining a multiple chamber thermal zone disposed to be in thermal communication with the receptacle and constructed and arranged to transmit thermal energy between each multiple chamber thermal zone and an associated region of the receptacle, wherein the associated region of the receptacle encompasses all or a portion of each of two or more but less than all chambers of the receptacle; and a controller programmed to control operation of the one or more thermal elements defining the multiple chamber thermal zones to selectively heat or cool the chambers encompassed within the regions associated with the multiple chamber thermal zones.
one or more thermal elements each defining a multiple chamber thermal zone disposed to be in thermal communication with the receptacle and constructed and arranged to transmit thermal energy between each multiple chamber thermal zone and an associated region of the receptacle, wherein the associated region of the receptacle encompasses all or a portion of each of two or more but less than all chambers of the receptacle; and a controller programmed to control operation of the one or more thermal elements defining the multiple chamber thermal zones to selectively heat or cool the chambers encompassed within the regions associated with the multiple chamber thermal zones.
2. The instrument of claim 1, further comprising one or more thermal elements defining one or more single chamber thermal zones disposed to be in thermal communication with the receptacle and constructed and arranged to transmit thermal energy between each single chamber thermal zone and an associated region of the receptacle encompassing all or a portion of one chamber of the receptacle, wherein the controller is programmed to control operation of the thermal elements defining the single chamber thermal zones to selectively heat or cool the chambers encompassed within the regions associated with the single chamber thermal zones.
3. The instrument of claim 1 or 2, wherein the thermal elements comprise one or more Peltier' devices controlled by the controller to selectively heat or cool a body with which the Peltier' device is in thermal contact.
4. The instrument of any one of claims 1 to 3, wherein the thermal elements comprise a heat transfer element formed from a thermally conductive material and having a peripheral shape corresponding to a predetermined shape of a multiple chamber thermal zone defined by the heat transfer element.
5. The instrument of claim 1 or 2, wherein the thermal elements comprise: one or more Peltier' devices controlled by the controller to selectively heat or cool a body with which the Peltier' devices are in thermal contact; and a heat transfer element associated with each multiple chamber thermal zone, said heat transfer element being formed from a thermally conductive material and having a generally flat surface and a peripheral shape corresponding to a predetermined shape of a multiple chamber thermal zone defined by the heat transfer element, wherein the Peltier' devices are in thermal contact with the heat transfer element.
6. The instrument of claim 4 or 5, wherein the heat transfer element is held within the instrument in a fixed position with respect to the receptacle.
7. The instrument of claim 4, 5 or 6, wherein the heat transfer element is formed from aluminum.
8. The instrument of claim 3 or 5, further comprising a heat dissipation element constructed and arranged to dissipate heat from the Peltier' devices.
9. The instrument of claim 8, wherein the heat dissipation element comprises a heat sink formed from a conductive material and including a block having one side in thermal communication with at least one Peltier' device and an opposite side from which heat dissipation fins extend from the block.
10. The instrument of claim 9, wherein the heat dissipation element further comprises a fan disposed adjacent the heat sink and configured to generate an air flow over the heat dissipation fins of the heat sink.
11. The instrument of claim 10, wherein operation of the fan is controlled by the controller.
12. The instrument of claim 9, 10 or 11, wherein the heat sink is formed from aluminum.
13. The instrument of any one of claims 1 to 12, wherein the instrument comprises multiple thermal elements, each thermal element being separated from other thermal elements by isolating structure comprising a thermally non-conductive material.
14. The instrument of claim 13, wherein the heat transfer element and the isolating structure are held within the instrument in fixed positions with respect to the receptacle.
15. The instrument of any one of claims 1 to 14, further comprising one or more temperature sensors for sensing the temperature of each thermal zone and communicating the sensed temperature to the controller.
16. The instrument of any one of claims 1 to 15, wherein the controller is configured to control the operation of the thermal elements to establish an ambient temperature within a prescribed temperature range.
17. The instrument of claim 16, wherein the prescribed temperature range is about 20°C to 40°C.
18. The instrument of claim 16, wherein the prescribed temperature range is about 25°C to 37°C.
19. The instrument of any one of claims 1 to 15, wherein the controller is configured to control operation of the thermal elements to heat one or more chambers to temperatures within a prescribed temperature range.
20. The instrument of claim 19, wherein the prescribed temperature range encompasses temperatures required to perform a process requiring thermal cycling.
21. The instrument of claim 19 or 20, wherein the prescribed temperature range is about 5°C to 95°C.
22. The instrument of claim 20, wherein the process is a PCR amplification reaction.
23. The instrument of any one of claims 1 to 15, wherein the controller is configured to control operation of the thermal elements to heat or cool the contents of chambers encompassed within a region associated with the multiple chamber thermal zone to a predetermined temperature for a predetermined period of time.
24. The instrument of any one of claims 1 to 23, wherein the one or more thermal elements define at least two multiple chamber thermal zones.
25. The instrument of any one of claims 1 to 24, wherein when a receptacle is supported in the operative position within the instrument, filling a chamber encompassed within the region associated with the multiple chamber thermal zone with fluid will increase thermal communication between the chamber and the multiple chamber thermal zone.
26. The instrument of any one of claims 1 to 25, wherein each of the one or more thermal elements defining a multiple chamber thermal zone comprises a metal plate having a size and shape encompassing all or a portion of each of two or more but less than all chambers of the receptacle.
27. A method for heating or cooling substances within a receptacle having a plurality of interconnected chambers, the method comprising the steps of:
positioning the receptacle in thermal communication with one or more multiple chamber thermal zones contained in an analyzer, each multiple chamber thermal zone being associated with a region of the receptacle encompassing all or a portion of each of two or more but less than all chambers of the receptacle; and transmitting thermal energy between each multiple chamber thermal zone and the chambers encompassed by the region associated with the multiple chamber thermal zone to selectively heat or cool substances contained within the encompassed chambers to a temperature different than the temperature of the chambers encompassed by at least one other region.
positioning the receptacle in thermal communication with one or more multiple chamber thermal zones contained in an analyzer, each multiple chamber thermal zone being associated with a region of the receptacle encompassing all or a portion of each of two or more but less than all chambers of the receptacle; and transmitting thermal energy between each multiple chamber thermal zone and the chambers encompassed by the region associated with the multiple chamber thermal zone to selectively heat or cool substances contained within the encompassed chambers to a temperature different than the temperature of the chambers encompassed by at least one other region.
28. The method of claim 27, further comprising positioning the receptacle in thermal communication with one or more single chamber thermal zones contained in the analyzer, each single chamber thermal zone being associated with a region of the receptacle encompassing all or a portion of one chamber of the receptacle.
29. The method of claim 27 or 28, wherein the transmitting step comprises heating or cooling the multiple chamber thermal zone with one or more Peltier' devices.
30. The method of claim 29, wherein the heat is transferred to/from the multiple chamber thermal zone from/to the Peltier' device using a thermal element.
31. The method of claim 30, wherein the thermal element comprises a metal plate having a size and shape encompassing all or a portion of each of two or more but less than all chambers of the receptacle.
32. The method of any one of claims 27 to 31, wherein ambient temperature of the analyzer is different than the temperature of substances contained within chambers encompassed by the regions of the receptacle associated with each multiple chamber thermal zone during the method.
33. The method of any one of claims 27 to 32, wherein the transmitting step comprises alternately heating and cooling at least one of the multiple chamber thermal zones.
34. The method of any one of claims 27 to 33, further comprising thermally separating each multiple chamber thermal zone from other multiple chamber thermal zones.
35. The method of any one of claims 27 to 34, further comprising dissipating heat from the multiple chamber thermal zone.
36. The method of any one of claims 27 to 35, further comprising sensing the temperature of each multiple chamber thermal zone.
37. The method of any one of claims 27 to 36, further comprising expanding a chamber encompassed by the region of the receptacle associated with a multiple chamber thermal zone to increase the thermal communication between the expanded chamber and the associated multiple chamber thermal zone.
38. The method of any one of claims 27 to 37, wherein the analyzer contains at least three multiple chamber thermal zones.
39. The method of any one of claims 27 to 38, wherein each of the plurality of interconnected chambers is adjacent to at least one other chamber of the receptacle.
40. The method of any one of claims 27 to 39, wherein each of the chambers of the receptacle comprises a flexible portion to facilitate substance movement between chambers.
41. The method of any one of claims 27 to 40, wherein the receptacle comprises opposed members, at least one of which members comprises a flexible sheet.
42. The method of claim 41, wherein each of the opposed members comprises a flexible sheet.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US94552007P | 2007-06-21 | 2007-06-21 | |
| US60/945,520 | 2007-06-21 | ||
| CA2691197A CA2691197C (en) | 2007-06-21 | 2008-06-20 | Instrument and receptacles for use in performing processes |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2691197A Division CA2691197C (en) | 2007-06-21 | 2008-06-20 | Instrument and receptacles for use in performing processes |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2743477A1 true CA2743477A1 (en) | 2008-12-31 |
| CA2743477C CA2743477C (en) | 2012-07-03 |
Family
ID=44318726
Family Applications (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2743404A Active CA2743404C (en) | 2007-06-21 | 2008-06-20 | Multi-chambered receptacles |
| CA2743365A Active CA2743365C (en) | 2007-06-21 | 2008-06-20 | Method of processing a sample in a receptacle having a plurality of interconnected chambers |
| CA2743405A Active CA2743405C (en) | 2007-06-21 | 2008-06-20 | Receptacles for storing substances in different physical states |
| CA2743477A Active CA2743477C (en) | 2007-06-21 | 2008-06-20 | Instruments and method for exposing a receptacle to multiple thermal zones |
Family Applications Before (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2743404A Active CA2743404C (en) | 2007-06-21 | 2008-06-20 | Multi-chambered receptacles |
| CA2743365A Active CA2743365C (en) | 2007-06-21 | 2008-06-20 | Method of processing a sample in a receptacle having a plurality of interconnected chambers |
| CA2743405A Active CA2743405C (en) | 2007-06-21 | 2008-06-20 | Receptacles for storing substances in different physical states |
Country Status (1)
| Country | Link |
|---|---|
| CA (4) | CA2743404C (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113265321A (en) * | 2021-06-08 | 2021-08-17 | 杭州霆科生物科技有限公司 | Micro-fluidic immunity and nucleic acid detection chip |
| CN113584136A (en) * | 2021-06-29 | 2021-11-02 | 广东粤港澳大湾区国家纳米科技创新研究院 | Capillary microfluidic qPCR nucleic acid detection device |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201519565D0 (en) * | 2015-11-05 | 2015-12-23 | Alere San Diego Inc | Sample preparation device |
| KR20210029824A (en) * | 2018-07-27 | 2021-03-16 | 아벡, 아이엔씨. | Disposable container and reaction system |
| CN114480306A (en) * | 2022-03-11 | 2022-05-13 | 兆丰华生物科技(福州)有限公司 | Cell passage method |
-
2008
- 2008-06-20 CA CA2743404A patent/CA2743404C/en active Active
- 2008-06-20 CA CA2743365A patent/CA2743365C/en active Active
- 2008-06-20 CA CA2743405A patent/CA2743405C/en active Active
- 2008-06-20 CA CA2743477A patent/CA2743477C/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113265321A (en) * | 2021-06-08 | 2021-08-17 | 杭州霆科生物科技有限公司 | Micro-fluidic immunity and nucleic acid detection chip |
| CN113584136A (en) * | 2021-06-29 | 2021-11-02 | 广东粤港澳大湾区国家纳米科技创新研究院 | Capillary microfluidic qPCR nucleic acid detection device |
Also Published As
| Publication number | Publication date |
|---|---|
| CA2743405C (en) | 2012-07-03 |
| CA2743365A1 (en) | 2008-12-31 |
| CA2743477C (en) | 2012-07-03 |
| CA2743404C (en) | 2012-11-06 |
| CA2743405A1 (en) | 2008-12-31 |
| CA2743365C (en) | 2012-07-03 |
| CA2743404A1 (en) | 2008-12-31 |
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| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |