CA2742675A1 - Perimeter septa enclosure - Google Patents
Perimeter septa enclosure Download PDFInfo
- Publication number
- CA2742675A1 CA2742675A1 CA 2742675 CA2742675A CA2742675A1 CA 2742675 A1 CA2742675 A1 CA 2742675A1 CA 2742675 CA2742675 CA 2742675 CA 2742675 A CA2742675 A CA 2742675A CA 2742675 A1 CA2742675 A1 CA 2742675A1
- Authority
- CA
- Canada
- Prior art keywords
- perimeter
- septa
- enclosure
- engagement
- tongue
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/22—Transparent or translucent parts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/26—Constructional details, e.g. recesses, hinges flexible
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- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
A sealed cell culture enclosure providing interior view by way of transparent panel members and providing general interior access by way of piercing tools through an enclosing perimeter septa member of pierceable, self sealing, resilient material.
Description
TITLE
PERIMETER SEPTA ENCLOSURE
FIELD OF THE INVENTION
A cell culture enclosure provides barrier isolation from an external environment and includes closure means for desired material transfer of selected cells, growth structures, nutrient media and other material.
Manual transfer between enclosures with wide closures such as a lid, screw cap or plug are usually performed in the facility of a clean room using prescribed tools and handling procedures, under an atmosphere of suitably filtered or prepared air thereby lessening exposure to contamination.
Manual transfer between enclosures without the facility of a clean room are generally performed through a self sealing elastomeric septa port utilizing a sterile piercing tool such as a needle or cannula thereby lessening exposure to contamination.
Enclosures such as a petri dish, slant or flask are well known in the art of cell culturing and provide spatial configurations suited to certain tasks.
A petri dish enclosure provides a large surface area for cell culture growth and differentiation, a wide transparent lid closure for general viewing and general interior access for material transfer; and requires a clean room environment to perform transfers reliably with a low rate of contamination.
It is the object of the present invention to provide an enclosure which includes the benefit of a large surface area for growth and differentiation, general viewing and general access for material transfer, without the need for a clean room to perform transfers reliably with a low rate of contamination.
SUMMARY OF THE INVENTION
The invention involves an assembled enclosure comprising panel members which are supported, sealed, and maintained by a perimeter septa member of pierceable self-sealing resilient material.
A perimeter septa member includes endless inward facing engagement elements while panel members include an endless outward facing engagement element; engagement of engagement elements forms a sealed enclosure.
A first method of the invention involves stretch fit of a perimeter septa member where the perimeter length of a perimeter septa engagement element is shorter than the perimeter length of a corresponding panel member engagement element; adaptive engagement of engagement elements by stretch fitting a perimeter septa engagement element over a panel member engagement element provides inward compression of engagement element surfaces thereby sealing.
A second method of the invention involves interference fit of engagement elements to provide a seal.
A third method of the invention involves a compression distribution element to distribute compressive sealing force along a non-convex or straight edge region of a panel member perimeter engagement element.
A forth method of the invention involves an adaptor for a petri dish.
The following prior art share similar elements with the present invention;
US2144255 Carpenter, shows needle access to a petri dish;
US2942520 Rose, shows septa access to a petri like dish;
US3928142 Smith, shows needle access through a resilient perimeter member;
US4094429 Urbin, shows an inside out resilient member;
US4133441 Mittleman et al., shows an inside out resilient member;
US4301252 Baker et al., shows septa access in a petri like dish;
US4435508 Gabridge, shows a resilient seal in a petri like dish;
US4932409 Hirschberg, shows an inside out resilient seal member;
US5179024 Dahms, shows a resilient perimeter septa;
US5324636 Bartos et al., shows a resilient perimeter seal;
US5565353 Klebe et al., shows desire for multiple septa access points;
US6030582 Levy, shows tongue and groove means to maintain a resilient septa;
US6410309 Barbera-Guillem et al., shows septa access in a petri like dish;
US6475777 Sarem et al., shows septa access for a cell culture dish.
PERIMETER SEPTA ENCLOSURE
FIELD OF THE INVENTION
A cell culture enclosure provides barrier isolation from an external environment and includes closure means for desired material transfer of selected cells, growth structures, nutrient media and other material.
Manual transfer between enclosures with wide closures such as a lid, screw cap or plug are usually performed in the facility of a clean room using prescribed tools and handling procedures, under an atmosphere of suitably filtered or prepared air thereby lessening exposure to contamination.
Manual transfer between enclosures without the facility of a clean room are generally performed through a self sealing elastomeric septa port utilizing a sterile piercing tool such as a needle or cannula thereby lessening exposure to contamination.
Enclosures such as a petri dish, slant or flask are well known in the art of cell culturing and provide spatial configurations suited to certain tasks.
A petri dish enclosure provides a large surface area for cell culture growth and differentiation, a wide transparent lid closure for general viewing and general interior access for material transfer; and requires a clean room environment to perform transfers reliably with a low rate of contamination.
It is the object of the present invention to provide an enclosure which includes the benefit of a large surface area for growth and differentiation, general viewing and general access for material transfer, without the need for a clean room to perform transfers reliably with a low rate of contamination.
SUMMARY OF THE INVENTION
The invention involves an assembled enclosure comprising panel members which are supported, sealed, and maintained by a perimeter septa member of pierceable self-sealing resilient material.
A perimeter septa member includes endless inward facing engagement elements while panel members include an endless outward facing engagement element; engagement of engagement elements forms a sealed enclosure.
A first method of the invention involves stretch fit of a perimeter septa member where the perimeter length of a perimeter septa engagement element is shorter than the perimeter length of a corresponding panel member engagement element; adaptive engagement of engagement elements by stretch fitting a perimeter septa engagement element over a panel member engagement element provides inward compression of engagement element surfaces thereby sealing.
A second method of the invention involves interference fit of engagement elements to provide a seal.
A third method of the invention involves a compression distribution element to distribute compressive sealing force along a non-convex or straight edge region of a panel member perimeter engagement element.
A forth method of the invention involves an adaptor for a petri dish.
The following prior art share similar elements with the present invention;
US2144255 Carpenter, shows needle access to a petri dish;
US2942520 Rose, shows septa access to a petri like dish;
US3928142 Smith, shows needle access through a resilient perimeter member;
US4094429 Urbin, shows an inside out resilient member;
US4133441 Mittleman et al., shows an inside out resilient member;
US4301252 Baker et al., shows septa access in a petri like dish;
US4435508 Gabridge, shows a resilient seal in a petri like dish;
US4932409 Hirschberg, shows an inside out resilient seal member;
US5179024 Dahms, shows a resilient perimeter septa;
US5324636 Bartos et al., shows a resilient perimeter seal;
US5565353 Klebe et al., shows desire for multiple septa access points;
US6030582 Levy, shows tongue and groove means to maintain a resilient septa;
US6410309 Barbera-Guillem et al., shows septa access in a petri like dish;
US6475777 Sarem et al., shows septa access for a cell culture dish.
BRIEF DESCRIPTION OF THE DRAWINGS
Preferred embodiments and relationships between elements are illustrated in the following figures:
FIG. 1, perspective view of a first embodiment of an assembled perimeter septa enclosure;
FIG. 2, a perspective and cutaway view of an assembled perimeter septa enclosure;
FIG. 3, a partial section view of adaptive engagement means;
FIG. 4, a partial section view of assembled adaptive engagement means;
FIG. 5, plan view of a second embodiment of a perimeter septa member;
FIG. 6, plan view of an inside out perimeter septa member of FIG. 5;
FIG. 7, a perspective view of panel member for the second embodiment;
FIG. 8, a perspective view of the second embodiment of a perimeter septa enclosure;
FIG. 9, a partial section view of a petri-dish adaptor.
Preferred embodiments and relationships between elements are illustrated in the following figures:
FIG. 1, perspective view of a first embodiment of an assembled perimeter septa enclosure;
FIG. 2, a perspective and cutaway view of an assembled perimeter septa enclosure;
FIG. 3, a partial section view of adaptive engagement means;
FIG. 4, a partial section view of assembled adaptive engagement means;
FIG. 5, plan view of a second embodiment of a perimeter septa member;
FIG. 6, plan view of an inside out perimeter septa member of FIG. 5;
FIG. 7, a perspective view of panel member for the second embodiment;
FIG. 8, a perspective view of the second embodiment of a perimeter septa enclosure;
FIG. 9, a partial section view of a petri-dish adaptor.
DESCRIPTION OF THE PREFERRED EMBODIMENTS
Referring to the figures, in FIG. 1 there is shown an assembled enclosure 20 with transparent upper and lower circular panel members 21 being separated, supported, enclosed and sealed within the openings of an endless perimeter septa member 22, as will be described further.
In FIG 2 a panel member 21 can provide a view into the perimeter septa enclosure or support a growth medium 50.
A perimeter septa member 22 formed from pierceable, self sealing, resilient material adaptively engages a panel member by tongue and groove means.
A needle shown at 60 has pierced a perimeter septa member 22.
In FIG. 3 a tongue element 24 includes the smooth perimeter margins 24A and perimeter edge face 24B of a panel member 21;
an endless inward facing groove element 25 is shown on the inner surface of a perimeter septa member 22.
In FIG. 4, a perimeter septa member groove element 25 is engaged with panel member tongue element 24;
a panel member is suspended and spatially maintained by its perimeter tongue element while engaged within a perimeter septa member groove element.
Stretch fit of a perimeter septa groove element over a panel member tongue element provides elastic rebound force to compress a resilient groove bottom surface 25B against a panel member edge face 24 thereby sealing.
In FIG. 3, an acute groove side angle 26 between a groove bottom surface 25B
and adjacent groove side surfaces 25A provides interference fit between a panel member tongue margin 24A and groove side surfaces and thereby seals.
An endless perimeter septa member molded from homogenous material with a uniform cross section provides inward compression when stretch fit over a panel member with a continuously convex or circular engagement element.
A compression distribution element is provided to distribute inward compression along non-convex regions of a panel member perimeter.
FIG. 5 shows a second embodiment of a perimeter septa member 32 adapted to provide inward compression against a rectangular panel member edge face 52 seen in FIG. 7;
a compression distribution element is shown as a gradual arch in thickness 31 between adjacent corner reinforcement elements 33;
the perimeter septa member 32 of FIG. 5 is turned inside out to provide an inverted perimeter septa member 42 of FIG. 6; groove elements 25 of the inverted perimeter septa member face inwards to engage a rectangular panel member tongue element;
a shorter inner perimeter 36 of the perimeter septa member 32 of FIG. 5 becomes the outer perimeter of the inverted perimeter septa member 42 of FIG. 6 and is in longitudinal tension;
a longer outer perimeter 37 of the perimeter septa member 32 of FIG. 5 becomes the inner perimeter of the inverted perimeter septa member 42 of FIG. 6 and is in longitudinal compression;
an inverted perimeter septa member groove element 25 of FIG. 6 is stretch fit over rectangular panel member tongue face 52 of FIG 7; corner locating elements 34 of FIG. 6 are aligning with rectangular panel member corner elements 53 of FIG. 7;
longitudinal tension in the outer perimeter of the inverted perimeter septa augmented by tension of a stretch fit flattens the compression distribution element and compresses a groove bottom surface 25B
of FIG. 3 against an edge face 52 of FIG. 7 of a rectangular panel member thereby forming the sealed enclosure of FIG. 8.
A correctly formed compression distribution element depends on the particular dimensions of a panel member, the dimensions of a perimeter septa member, as well as the properties of perimeter septa member material composition.
In FIG. 9, a third embodiment shows an adaptor between top and bottom halves of a petri dish 61 comprising; an endless perimeter septa member 62 having endless peripheral groove elements 65;
adaptive engagement is by interference fit of a petri dish rim margin 64 into a perimeter septa groove element 65; elastic rebound of a perimeter septa groove element compresses groove side surfaces against a petri dish rim margin surface and thereby seals.
Referring to the figures, in FIG. 1 there is shown an assembled enclosure 20 with transparent upper and lower circular panel members 21 being separated, supported, enclosed and sealed within the openings of an endless perimeter septa member 22, as will be described further.
In FIG 2 a panel member 21 can provide a view into the perimeter septa enclosure or support a growth medium 50.
A perimeter septa member 22 formed from pierceable, self sealing, resilient material adaptively engages a panel member by tongue and groove means.
A needle shown at 60 has pierced a perimeter septa member 22.
In FIG. 3 a tongue element 24 includes the smooth perimeter margins 24A and perimeter edge face 24B of a panel member 21;
an endless inward facing groove element 25 is shown on the inner surface of a perimeter septa member 22.
In FIG. 4, a perimeter septa member groove element 25 is engaged with panel member tongue element 24;
a panel member is suspended and spatially maintained by its perimeter tongue element while engaged within a perimeter septa member groove element.
Stretch fit of a perimeter septa groove element over a panel member tongue element provides elastic rebound force to compress a resilient groove bottom surface 25B against a panel member edge face 24 thereby sealing.
In FIG. 3, an acute groove side angle 26 between a groove bottom surface 25B
and adjacent groove side surfaces 25A provides interference fit between a panel member tongue margin 24A and groove side surfaces and thereby seals.
An endless perimeter septa member molded from homogenous material with a uniform cross section provides inward compression when stretch fit over a panel member with a continuously convex or circular engagement element.
A compression distribution element is provided to distribute inward compression along non-convex regions of a panel member perimeter.
FIG. 5 shows a second embodiment of a perimeter septa member 32 adapted to provide inward compression against a rectangular panel member edge face 52 seen in FIG. 7;
a compression distribution element is shown as a gradual arch in thickness 31 between adjacent corner reinforcement elements 33;
the perimeter septa member 32 of FIG. 5 is turned inside out to provide an inverted perimeter septa member 42 of FIG. 6; groove elements 25 of the inverted perimeter septa member face inwards to engage a rectangular panel member tongue element;
a shorter inner perimeter 36 of the perimeter septa member 32 of FIG. 5 becomes the outer perimeter of the inverted perimeter septa member 42 of FIG. 6 and is in longitudinal tension;
a longer outer perimeter 37 of the perimeter septa member 32 of FIG. 5 becomes the inner perimeter of the inverted perimeter septa member 42 of FIG. 6 and is in longitudinal compression;
an inverted perimeter septa member groove element 25 of FIG. 6 is stretch fit over rectangular panel member tongue face 52 of FIG 7; corner locating elements 34 of FIG. 6 are aligning with rectangular panel member corner elements 53 of FIG. 7;
longitudinal tension in the outer perimeter of the inverted perimeter septa augmented by tension of a stretch fit flattens the compression distribution element and compresses a groove bottom surface 25B
of FIG. 3 against an edge face 52 of FIG. 7 of a rectangular panel member thereby forming the sealed enclosure of FIG. 8.
A correctly formed compression distribution element depends on the particular dimensions of a panel member, the dimensions of a perimeter septa member, as well as the properties of perimeter septa member material composition.
In FIG. 9, a third embodiment shows an adaptor between top and bottom halves of a petri dish 61 comprising; an endless perimeter septa member 62 having endless peripheral groove elements 65;
adaptive engagement is by interference fit of a petri dish rim margin 64 into a perimeter septa groove element 65; elastic rebound of a perimeter septa groove element compresses groove side surfaces against a petri dish rim margin surface and thereby seals.
Claims (8)
1. A sealed enclosure for cell culture work comprising;
at least two rigid panel members; a said panel member being contiguous with an outer perimeter;
said outer perimeter being contiguous with an endless engagement element of tongue or groove means;
a perimeter septa member formed of pierceable, self sealing, resilient material; said perimeter septa member encompassing a volume by means of an endless circuit; said endless circuit including at least two openings; said openings include a peripheral margin; said peripheral margin being proximate and contiguous with an endless engagement element of tongue or groove means;
a sealed enclosure being assembled and formed by adaptive engagement of said panel members engagement elements with respective said perimeter septa member engagement elements.
at least two rigid panel members; a said panel member being contiguous with an outer perimeter;
said outer perimeter being contiguous with an endless engagement element of tongue or groove means;
a perimeter septa member formed of pierceable, self sealing, resilient material; said perimeter septa member encompassing a volume by means of an endless circuit; said endless circuit including at least two openings; said openings include a peripheral margin; said peripheral margin being proximate and contiguous with an endless engagement element of tongue or groove means;
a sealed enclosure being assembled and formed by adaptive engagement of said panel members engagement elements with respective said perimeter septa member engagement elements.
2. An enclosure as defined in claim 1 wherein a said perimeter septa member engagement element is stretch fit around said panel member engagement element.
3. An enclosure as defined in claim 1 wherein adaptive engagement includes interference fit of a said tongue element into a said groove element.
4. An enclosure as defined in claim 1 wherein a said panel member includes a said perimeter tongue element; said perimeter septa member includes said groove elements.
5. An enclosure as defined in claim 1 wherein a said panel member includes a said perimeter groove element; said perimeter septa member includes said tongue elements.
6. An enclosure as defined in claim 1 wherein said panel members include the halves of a petri dish.
7. An enclosure as defined in claim 1 wherein a said perimeter septa member includes a compression distribution element comprising;
an arch shape formed in a resilient material whereupon said arch shape is stretched against a surface to direct and distribute compression against said surface.
an arch shape formed in a resilient material whereupon said arch shape is stretched against a surface to direct and distribute compression against said surface.
8. An enclosure as defined in claim 1 wherein a said perimeter septa member is utilized after being turned inside out.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA 2742675 CA2742675A1 (en) | 2011-06-10 | 2011-06-10 | Perimeter septa enclosure |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA 2742675 CA2742675A1 (en) | 2011-06-10 | 2011-06-10 | Perimeter septa enclosure |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2742675A1 true CA2742675A1 (en) | 2012-12-10 |
Family
ID=47352195
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA 2742675 Abandoned CA2742675A1 (en) | 2011-06-10 | 2011-06-10 | Perimeter septa enclosure |
Country Status (1)
| Country | Link |
|---|---|
| CA (1) | CA2742675A1 (en) |
-
2011
- 2011-06-10 CA CA 2742675 patent/CA2742675A1/en not_active Abandoned
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Dead |
Effective date: 20170612 |