CA2712213C - Methode pour realiser une separation bidimensionnelle limitee de proteines et d'autres matieres biologiques - Google Patents

Methode pour realiser une separation bidimensionnelle limitee de proteines et d'autres matieres biologiques Download PDF

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Publication number
CA2712213C
CA2712213C CA2712213A CA2712213A CA2712213C CA 2712213 C CA2712213 C CA 2712213C CA 2712213 A CA2712213 A CA 2712213A CA 2712213 A CA2712213 A CA 2712213A CA 2712213 C CA2712213 C CA 2712213C
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Canada
Prior art keywords
proteins
protein
separation
zone
separation stage
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Active
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CA2712213A
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English (en)
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CA2712213A1 (fr
Inventor
Tiemin Huang
Jiaqi Wu
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ProteinSimple
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ProteinSimple
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Priority to CA3123719A priority Critical patent/CA3123719A1/fr
Priority to CA2712213A priority patent/CA2712213C/fr
Publication of CA2712213A1 publication Critical patent/CA2712213A1/fr
Application granted granted Critical
Publication of CA2712213C publication Critical patent/CA2712213C/fr
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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44795—Isoelectric focusing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/74—Optical detectors
    • G01N2030/746—Optical detectors detecting along the line of flow, e.g. axial
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G01N2030/8831—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins

Abstract

Un procédé et un appareil permettent deffectuer une focalisation isoélectrique capillaire suivie dune mobilisation des zones focalisées par un écoulement hydrodynamique induit ou une mobilisation chimique. Ces deux dimensions de séparation sont intégrées à une détection délectrophorèse sur un canal entier en temps réel et une injection déchantillon automatique pour obtenir une résolution de séparation supérieure à celle pouvant être obtenue à laide dagencements bidimensionnels capillaires orthogonaux connus.
CA2712213A 2010-08-05 2010-08-05 Methode pour realiser une separation bidimensionnelle limitee de proteines et d'autres matieres biologiques Active CA2712213C (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CA3123719A CA3123719A1 (fr) 2010-08-05 2010-08-05 Methode pour realiser une separation bidimensionnelle limitee de proteines et d'autres matieres biologiques
CA2712213A CA2712213C (fr) 2010-08-05 2010-08-05 Methode pour realiser une separation bidimensionnelle limitee de proteines et d'autres matieres biologiques

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CA2712213A CA2712213C (fr) 2010-08-05 2010-08-05 Methode pour realiser une separation bidimensionnelle limitee de proteines et d'autres matieres biologiques

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA3123719A Division CA3123719A1 (fr) 2010-08-05 2010-08-05 Methode pour realiser une separation bidimensionnelle limitee de proteines et d'autres matieres biologiques

Publications (2)

Publication Number Publication Date
CA2712213A1 CA2712213A1 (fr) 2012-02-05
CA2712213C true CA2712213C (fr) 2021-08-24

Family

ID=45566691

Family Applications (2)

Application Number Title Priority Date Filing Date
CA3123719A Pending CA3123719A1 (fr) 2010-08-05 2010-08-05 Methode pour realiser une separation bidimensionnelle limitee de proteines et d'autres matieres biologiques
CA2712213A Active CA2712213C (fr) 2010-08-05 2010-08-05 Methode pour realiser une separation bidimensionnelle limitee de proteines et d'autres matieres biologiques

Family Applications Before (1)

Application Number Title Priority Date Filing Date
CA3123719A Pending CA3123719A1 (fr) 2010-08-05 2010-08-05 Methode pour realiser une separation bidimensionnelle limitee de proteines et d'autres matieres biologiques

Country Status (1)

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CA (2) CA3123719A1 (fr)

Also Published As

Publication number Publication date
CA2712213A1 (fr) 2012-02-05
CA3123719A1 (fr) 2012-02-05

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