CA2690651C - Preservation des acides nucleiques foetaux dans le plasma maternel - Google Patents
Preservation des acides nucleiques foetaux dans le plasma maternel Download PDFInfo
- Publication number
- CA2690651C CA2690651C CA2690651A CA2690651A CA2690651C CA 2690651 C CA2690651 C CA 2690651C CA 2690651 A CA2690651 A CA 2690651A CA 2690651 A CA2690651 A CA 2690651A CA 2690651 C CA2690651 C CA 2690651C
- Authority
- CA
- Canada
- Prior art keywords
- blood sample
- maternal blood
- fetal
- nucleic acid
- nucleic acids
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 230000001605 fetal effect Effects 0.000 title claims abstract description 166
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 159
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 159
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 159
- 230000008774 maternal effect Effects 0.000 title claims abstract description 128
- 238000004321 preservation Methods 0.000 title description 13
- 239000008280 blood Substances 0.000 claims abstract description 170
- 210000004369 blood Anatomy 0.000 claims abstract description 169
- 238000000034 method Methods 0.000 claims abstract description 64
- 210000000601 blood cell Anatomy 0.000 claims abstract description 21
- 230000000694 effects Effects 0.000 claims abstract description 16
- 239000003223 protective agent Substances 0.000 claims description 92
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims description 60
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 34
- ZCTXEAQXZGPWFG-UHFFFAOYSA-N imidurea Chemical compound O=C1NC(=O)N(CO)C1NC(=O)NCNC(=O)NC1C(=O)NC(=O)N1CO ZCTXEAQXZGPWFG-UHFFFAOYSA-N 0.000 claims description 34
- 239000003146 anticoagulant agent Substances 0.000 claims description 20
- 229940127219 anticoagulant drug Drugs 0.000 claims description 19
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 18
- 239000004471 Glycine Substances 0.000 claims description 15
- 238000000576 coating method Methods 0.000 claims description 15
- 238000003752 polymerase chain reaction Methods 0.000 claims description 15
- 239000011248 coating agent Substances 0.000 claims description 14
- 101710163270 Nuclease Proteins 0.000 claims description 10
- 238000007710 freezing Methods 0.000 claims description 9
- 230000008014 freezing Effects 0.000 claims description 9
- 102000004169 proteins and genes Human genes 0.000 claims description 9
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 claims description 6
- 229960002897 heparin Drugs 0.000 claims description 6
- 229920000669 heparin Polymers 0.000 claims description 6
- 238000009609 prenatal screening Methods 0.000 claims description 4
- 102000004190 Enzymes Human genes 0.000 claims description 3
- 108090000790 Enzymes Proteins 0.000 claims description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 claims description 2
- 108091005461 Nucleic proteins Proteins 0.000 claims description 2
- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 claims description 2
- 229930040373 Paraformaldehyde Natural products 0.000 claims description 2
- 238000000246 agarose gel electrophoresis Methods 0.000 claims description 2
- 238000004132 cross linking Methods 0.000 claims description 2
- 229920002866 paraformaldehyde Polymers 0.000 claims description 2
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 claims 1
- 229910052710 silicon Inorganic materials 0.000 claims 1
- 239000010703 silicon Substances 0.000 claims 1
- 230000006037 cell lysis Effects 0.000 abstract description 16
- 102000016911 Deoxyribonucleases Human genes 0.000 abstract description 11
- 108010053770 Deoxyribonucleases Proteins 0.000 abstract description 11
- 238000012545 processing Methods 0.000 abstract description 10
- 102000006382 Ribonucleases Human genes 0.000 abstract description 9
- 108010083644 Ribonucleases Proteins 0.000 abstract description 9
- 230000001965 increasing effect Effects 0.000 abstract description 8
- 108020004414 DNA Proteins 0.000 description 135
- 210000002381 plasma Anatomy 0.000 description 107
- 239000000523 sample Substances 0.000 description 94
- 238000012360 testing method Methods 0.000 description 21
- 210000004027 cell Anatomy 0.000 description 18
- 239000000203 mixture Substances 0.000 description 17
- 238000002955 isolation Methods 0.000 description 16
- 230000007423 decrease Effects 0.000 description 13
- 239000004480 active ingredient Substances 0.000 description 12
- 239000000834 fixative Substances 0.000 description 12
- 229960002449 glycine Drugs 0.000 description 12
- FYZXEMANQYHCFX-UHFFFAOYSA-K tripotassium;2-[2-[bis(carboxylatomethyl)amino]ethyl-(carboxymethyl)amino]acetate Chemical compound [K+].[K+].[K+].OC(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O FYZXEMANQYHCFX-UHFFFAOYSA-K 0.000 description 11
- 230000001413 cellular effect Effects 0.000 description 10
- 238000011534 incubation Methods 0.000 description 10
- 238000003753 real-time PCR Methods 0.000 description 10
- 230000008569 process Effects 0.000 description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 8
- 238000004458 analytical method Methods 0.000 description 8
- 239000007788 liquid Substances 0.000 description 8
- 239000003755 preservative agent Substances 0.000 description 8
- 108090000623 proteins and genes Proteins 0.000 description 8
- 230000003321 amplification Effects 0.000 description 7
- 238000003199 nucleic acid amplification method Methods 0.000 description 7
- 235000018102 proteins Nutrition 0.000 description 7
- -1 Mg" Chemical class 0.000 description 6
- 230000015556 catabolic process Effects 0.000 description 6
- 238000005119 centrifugation Methods 0.000 description 6
- 238000006731 degradation reaction Methods 0.000 description 6
- SOROIESOUPGGFO-UHFFFAOYSA-N diazolidinylurea Chemical compound OCNC(=O)N(CO)C1N(CO)C(=O)N(CO)C1=O SOROIESOUPGGFO-UHFFFAOYSA-N 0.000 description 6
- 229960001083 diazolidinylurea Drugs 0.000 description 6
- 238000013412 genome amplification Methods 0.000 description 6
- 238000011002 quantification Methods 0.000 description 6
- 239000007787 solid Substances 0.000 description 6
- 229940122426 Nuclease inhibitor Drugs 0.000 description 5
- 230000002759 chromosomal effect Effects 0.000 description 5
- 239000004615 ingredient Substances 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 238000005259 measurement Methods 0.000 description 5
- 238000003793 prenatal diagnosis Methods 0.000 description 5
- 108091061744 Cell-free fetal DNA Proteins 0.000 description 4
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 4
- 208000026350 Inborn Genetic disease Diseases 0.000 description 4
- 208000024556 Mendelian disease Diseases 0.000 description 4
- 238000011529 RT qPCR Methods 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 4
- 239000013611 chromosomal DNA Substances 0.000 description 4
- 230000002939 deleterious effect Effects 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 4
- 230000003631 expected effect Effects 0.000 description 4
- 210000003754 fetus Anatomy 0.000 description 4
- 210000000265 leukocyte Anatomy 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 206010008805 Chromosomal abnormalities Diseases 0.000 description 3
- 208000031404 Chromosome Aberrations Diseases 0.000 description 3
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 3
- 238000000692 Student's t-test Methods 0.000 description 3
- 230000005856 abnormality Effects 0.000 description 3
- 230000006907 apoptotic process Effects 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 230000030833 cell death Effects 0.000 description 3
- 108091092356 cellular DNA Proteins 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 230000002068 genetic effect Effects 0.000 description 3
- 239000003112 inhibitor Substances 0.000 description 3
- 239000003550 marker Substances 0.000 description 3
- 239000013612 plasmid Substances 0.000 description 3
- 230000001681 protective effect Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 108091008146 restriction endonucleases Proteins 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 210000002966 serum Anatomy 0.000 description 3
- 230000000087 stabilizing effect Effects 0.000 description 3
- 125000000143 2-carboxyethyl group Chemical group [H]OC(=O)C([H])([H])C([H])([H])* 0.000 description 2
- 108700028369 Alleles Proteins 0.000 description 2
- JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical compound [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 description 2
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 2
- 108010067770 Endopeptidase K Proteins 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- XYFCBTPGUUZFHI-UHFFFAOYSA-N Phosphine Chemical compound P XYFCBTPGUUZFHI-UHFFFAOYSA-N 0.000 description 2
- 206010034962 Photopsia Diseases 0.000 description 2
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- 210000002593 Y chromosome Anatomy 0.000 description 2
- ORILYTVJVMAKLC-UHFFFAOYSA-N adamantane Chemical group C1C(C2)CC3CC1CC2C3 ORILYTVJVMAKLC-UHFFFAOYSA-N 0.000 description 2
- 229910001573 adamantine Inorganic materials 0.000 description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 235000011130 ammonium sulphate Nutrition 0.000 description 2
- GIXWDMTZECRIJT-UHFFFAOYSA-N aurintricarboxylic acid Chemical compound C1=CC(=O)C(C(=O)O)=CC1=C(C=1C=C(C(O)=CC=1)C(O)=O)C1=CC=C(O)C(C(O)=O)=C1 GIXWDMTZECRIJT-UHFFFAOYSA-N 0.000 description 2
- 230000004888 barrier function Effects 0.000 description 2
- 229910000278 bentonite Inorganic materials 0.000 description 2
- 239000000440 bentonite Substances 0.000 description 2
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 description 2
- 239000010836 blood and blood product Substances 0.000 description 2
- 229940125691 blood product Drugs 0.000 description 2
- 201000011510 cancer Diseases 0.000 description 2
- 150000001768 cations Chemical class 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 239000000356 contaminant Substances 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 229920001577 copolymer Polymers 0.000 description 2
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 2
- 235000018417 cysteine Nutrition 0.000 description 2
- 230000009089 cytolysis Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000002405 diagnostic procedure Methods 0.000 description 2
- FFYPMLJYZAEMQB-UHFFFAOYSA-N diethyl pyrocarbonate Chemical compound CCOC(=O)OC(=O)OCC FFYPMLJYZAEMQB-UHFFFAOYSA-N 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- VHJLVAABSRFDPM-ZXZARUISSA-N dithioerythritol Chemical compound SC[C@H](O)[C@H](O)CS VHJLVAABSRFDPM-ZXZARUISSA-N 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 230000002209 hydrophobic effect Effects 0.000 description 2
- 125000005699 methyleneoxy group Chemical group [H]C([H])([*:1])O[*:2] 0.000 description 2
- 108700019599 monomethylolglycine Proteins 0.000 description 2
- 150000002917 oxazolidines Chemical class 0.000 description 2
- QUBQYFYWUJJAAK-UHFFFAOYSA-N oxymethurea Chemical compound OCNC(=O)NCO QUBQYFYWUJJAAK-UHFFFAOYSA-N 0.000 description 2
- 229950005308 oxymethurea Drugs 0.000 description 2
- 150000002989 phenols Chemical class 0.000 description 2
- 229910000065 phosphene Inorganic materials 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 238000007858 polymerase cycling assembly Methods 0.000 description 2
- 238000009598 prenatal testing Methods 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 238000003757 reverse transcription PCR Methods 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 229940101011 sodium hydroxymethylglycinate Drugs 0.000 description 2
- CITBNDNUEPMTFC-UHFFFAOYSA-M sodium;2-(hydroxymethylamino)acetate Chemical compound [Na+].OCNCC([O-])=O CITBNDNUEPMTFC-UHFFFAOYSA-M 0.000 description 2
- 239000007858 starting material Substances 0.000 description 2
- 238000007619 statistical method Methods 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 230000002195 synergetic effect Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 2
- 206010061765 Chromosomal mutation Diseases 0.000 description 1
- 208000016718 Chromosome Inversion Diseases 0.000 description 1
- 230000004544 DNA amplification Effects 0.000 description 1
- 238000007399 DNA isolation Methods 0.000 description 1
- ZRALSGWEFCBTJO-UHFFFAOYSA-N Guanidine Chemical class NC(N)=N ZRALSGWEFCBTJO-UHFFFAOYSA-N 0.000 description 1
- 208000028782 Hereditary disease Diseases 0.000 description 1
- 108060004795 Methyltransferase Proteins 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 238000010222 PCR analysis Methods 0.000 description 1
- 239000012807 PCR reagent Substances 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 208000002787 Pregnancy Complications Diseases 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 239000002318 adhesion promoter Substances 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 238000002669 amniocentesis Methods 0.000 description 1
- 229940035674 anesthetics Drugs 0.000 description 1
- 208000036878 aneuploidy Diseases 0.000 description 1
- 231100001075 aneuploidy Toxicity 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 229940127090 anticoagulant agent Drugs 0.000 description 1
- 239000000739 antihistaminic agent Substances 0.000 description 1
- 229940125715 antihistaminic agent Drugs 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 239000003613 bile acid Substances 0.000 description 1
- 239000000090 biomarker Substances 0.000 description 1
- 229920001400 block copolymer Polymers 0.000 description 1
- 230000023555 blood coagulation Effects 0.000 description 1
- 239000005388 borosilicate glass Substances 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 238000005251 capillar electrophoresis Methods 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 210000004252 chorionic villi Anatomy 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 230000002301 combined effect Effects 0.000 description 1
- 230000001010 compromised effect Effects 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 239000012149 elution buffer Substances 0.000 description 1
- 238000001917 fluorescence detection Methods 0.000 description 1
- 229920002313 fluoropolymer Polymers 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 238000013467 fragmentation Methods 0.000 description 1
- 238000006062 fragmentation reaction Methods 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 239000003193 general anesthetic agent Substances 0.000 description 1
- 229920000578 graft copolymer Polymers 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 238000007403 mPCR Methods 0.000 description 1
- 238000004949 mass spectrometry Methods 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 230000011987 methylation Effects 0.000 description 1
- 238000007069 methylation reaction Methods 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- TVMXDCGIABBOFY-UHFFFAOYSA-N octane Chemical compound CCCCCCCC TVMXDCGIABBOFY-UHFFFAOYSA-N 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 229920000139 polyethylene terephthalate Polymers 0.000 description 1
- 239000005020 polyethylene terephthalate Substances 0.000 description 1
- 229920000098 polyolefin Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 1
- 239000004810 polytetrafluoroethylene Substances 0.000 description 1
- 229920000915 polyvinyl chloride Polymers 0.000 description 1
- 239000004800 polyvinyl chloride Substances 0.000 description 1
- 201000011461 pre-eclampsia Diseases 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000035935 pregnancy Effects 0.000 description 1
- 208000012113 pregnancy disease Diseases 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 239000011253 protective coating Substances 0.000 description 1
- 239000012266 salt solution Substances 0.000 description 1
- 150000003839 salts Chemical group 0.000 description 1
- 150000004756 silanes Chemical class 0.000 description 1
- 229920005573 silicon-containing polymer Polymers 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 238000012956 testing procedure Methods 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 230000005945 translocation Effects 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
- 238000000870 ultraviolet spectroscopy Methods 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Un procédé de préservation et de traitement des acides nucléiques ftaux situés à lintérieur du plasma maternel est décrit, dans lequel un échantillon de sang maternel contenant des acides nucléiques ftaux est traité pour réduire à la fois la lyse cellulaire des cellules sanguines maternelles et lactivité de la désoxyribonucléase (DNase) et de la ribonucléase (RNase) à lintérieur des acides nucléiques ftaux. Le traitement de léchantillon aide à augmenter la quantité dacides nucléiques ftaux qui peuvent être identifiés et analysés tout en conservant la structure et lintégrité des acides nucléiques ftaux.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA2938275A CA2938275C (fr) | 2009-07-22 | 2010-01-21 | Compositions destinees a la preservation d'acides nucleiques foetaux dans les echantillons de sang maternel |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US22752909P | 2009-07-22 | 2009-07-22 | |
| US61/227,529 | 2009-07-22 |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2938275A Division CA2938275C (fr) | 2009-07-22 | 2010-01-21 | Compositions destinees a la preservation d'acides nucleiques foetaux dans les echantillons de sang maternel |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2690651A1 CA2690651A1 (fr) | 2011-01-22 |
| CA2690651C true CA2690651C (fr) | 2016-10-04 |
Family
ID=43495920
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2938275A Active CA2938275C (fr) | 2009-07-22 | 2010-01-21 | Compositions destinees a la preservation d'acides nucleiques foetaux dans les echantillons de sang maternel |
| CA2690651A Active CA2690651C (fr) | 2009-07-22 | 2010-01-21 | Preservation des acides nucleiques foetaux dans le plasma maternel |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2938275A Active CA2938275C (fr) | 2009-07-22 | 2010-01-21 | Compositions destinees a la preservation d'acides nucleiques foetaux dans les echantillons de sang maternel |
Country Status (1)
| Country | Link |
|---|---|
| CA (2) | CA2938275C (fr) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10017807B2 (en) * | 2013-03-15 | 2018-07-10 | Verinata Health, Inc. | Generating cell-free DNA libraries directly from blood |
-
2010
- 2010-01-21 CA CA2938275A patent/CA2938275C/fr active Active
- 2010-01-21 CA CA2690651A patent/CA2690651C/fr active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CA2938275C (fr) | 2021-08-31 |
| CA2938275A1 (fr) | 2011-01-22 |
| CA2690651A1 (fr) | 2011-01-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20240060113A1 (en) | Preservation of fetal nucleic acids in maternal plasma | |
| US11761025B2 (en) | Preservation of cell-free nucleic acids | |
| JP6750178B2 (ja) | 生物試料の安定化 | |
| CA2690651C (fr) | Preservation des acides nucleiques foetaux dans le plasma maternel | |
| US20230090569A1 (en) | Kits and methods for extracting nucleic acids from complex samples kits and methods for extracting nucleic acids from complex samples | |
| JP7395254B2 (ja) | 微生物の検出方法 | |
| Boyanton Jr et al. | Sample collection, processing, and storage for molecular genetic testing | |
| DE202010018561U1 (de) | Blutsammelröhrchen |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |