CA2660056A1 - Use of equol for ameliorating or preventing neuropsychiatric and neurodegenerative diseases or disorders - Google Patents

Abstract

The invention is directed to a method of preventing or ameliorating a neuropsychiatric or neurodegenerative disease or disorder in a subject. The method includes administering a composition comprising equol in an amount sufficient to prevent or ameliorate the neuropsychiatric or neurodegenerative disease or disorder. The equol may be a racemic mixture of R-equol and S-equol. The equol may be enantiomerically enriched with R-equol or enantiomerically enriched with S-equol.

Description

USE OF EQUOL FOR AMELIORATING OR PREVENTING
NEUROPSYCHIATRIC AND NEURODEGENERATIVE
DISEASES OR DISORDERS
FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT

[0001] This invention was made with Government support under Grant No.
NRI 2002-00798 and Grant No. NRI 2004-01811, awarded by the U.S. Dept. of Agriculture (USDA).

[0002] The Government has certain rights in this invention.
BACKGROUND

1. Technical Field [0003] This invention relates to equol and its use as a therapeutic compound for treating and preventing physiological and pathophysiological conditions medi-ated by androgens. Specifically, the present invention relates to the use of equol for preventing or treating neuropsychiatric or neurodegenerative diseases or disor-ders.

2. Background Information [0004] In recent years phytoestrogens have received increased investigative attention due to their potential protective effects against age-related diseases (e.g.
cardiovascular disease and osteoporosis) and hormone-dependent cancers (i.e., breast and prostate cancer). There are three main classifications of phytoestro-gens: 1) isoflavones (derived principally from soybeans), 2) lignans (found in flax-seed in large quantities) and 3) coumestans (derived from sprouting plants like al-falfa). Of these three main classifications, human consumption of isoflavones has the largest impact due to its availability and variety in food products containing soy. Of the isoflavones, genistein and daidzein are thought to exert the most po-tent estrogenic hormone activity and thus most attention has been directed toward these molecules (Knight D. C. et al., Obstet Gyneco, 187:897-904, (1996);
Setchell, K. D. R. Am J Clin Nutt , 129:1333S-1346S (1998); Kurzer, M. S. et al., Annu Rev Nutr, 17:353-381(1997)). However, these isoflavone molecules do not exist at high levels in their biologically active form in soy foods, but rather are at high abundance in a precursor form. For example, genistin, the precursor of gen-istein, is the glycosidic form that contains a carbohydrate portion of the molecule.
Additionally, malonylglucoside and acetylglucoside forms also are found. These conjugates are metabolized in the GI tract by intestinal bacteria, which hydrolyze the carbohydrate moiety to the biologically active phytoestrogen, genistein.
The same metabolic step occurs for the aglycone daidzein, which is converted from the glycosidic form daidzein. Daidzein is then further metabolized to equol in an "equol-producing" mammal. Thereafter, equol circulates in the blood stream at very high concentrations. Equol is not normally present in the urine of most healthy adults unless soy is consumed. The formation of equol in vivo is exclu-sively dependent on intestinal microflora as evidenced from the finding that germ-Phyto-Free animals do not excrete equol, and that equol is not found in the plasma and urine of newborn or 4-month old infants fed exclusively soy foods from birth due to the fat that the intestinal flora has not yet developed in neonates.
See Setchell K. D. R. et al., The Lancet 1997; 350:23-27.

[0005] The phenolic ring structures of isoflavones enable these compounds to bind estrogen receptors (ER) and mimic estrogen. Although genistein and daidzein bind to ER, it is with a lower affinity when compared to estradiol, and with a greater affinity for ER(3 than to ERa. Additionally, phytoestrogens have been reported to act like natural selective estrogen receptor modulators (SERMs) at various tissue sites throughout the body. In some tissues, there is evidence that phytoestrogens act as estrogen agonists, whereas in others, they display antagonis-tic characteristics comparable to that of tamoxifen or raloxifene where SERM
ac-tivity appears to be sex-hormone and gender dependent.

[0006] While the bulk of the scientific literature has focused on the natural isoflavones in soy or clover, little has been reported on the actions or effects of their intestinally derived metabolites.

[0007] Equol (7-hydroxy-3(4'hydroxyphenyl)-chroman) represents the major metabolite of the phytoestrogen daidzein, one of the main isoflavones found abun-dantly in soybeans and soy-foods. Equol, however, is not a phytoestrogen, be-cause it is not a natural constituent of plants. Equol does not occur naturally in any plant-based products. Rather, it is a non-steroidal isoflavone that is exclu-sively a product of intestinal bacterial metabolism (relatively few individuals, -30-400%, have the micro flora necessary to convert soy isoflavones to equol).
Previ-ous research with equol has identified that equol possess some weak estrogenic properties, binds sex hormone binding globulin, binds a-fetoprotein, and has anti-oxidant activity. However, equol is unique among the plant-derived isoflavones in that it possesses a chiral center and as such exists as two distinct enantiomeric forms, the R- and S-enantiomers. We have shown that the S-enantiomer of equol is the exclusive equol forin found in the urine and plasma of "equol-producing"
mammals consuming soy, and is the only equol enantiomer made by human intes-tinal bacteria. All previous studies on equol appear to have been conducted with the racemic forin of equol. There has in general been a lack of appreciation that two forms of equol exist and to our knowledge no previous study has reported on the specific actions or activity of the individual enantiomers. The R- and S-enantiomers conformationally differ, which subsequently influences their biologi-cal activity. For example, only the S-enantiomer of equol binds estrogen receptor (ER) with sufficient affinity to make it relevant to bind circulating equol levels reported in humans. Compared to 17(3-estradiol the relative binding affinities of the R- and S-equol enantiomer for ERa are 210.6 and 49.2 fold less respectively.
However, the S-equol enantiomer seems to be largely ER(3-selective with a rela-tively high affinity for ERP. Enantiomer S-equol binds ER(3 at approximately 20% that of 170-estradiol [equol, Kd=0.7 nM vs. 17(3-estradiol, Kd=0.15 nM], while the R-equol enantiomer binds at approximately 100 fold less. R-Equol, al-though not naturally occurring, is of considerable importance because of its ability to modulate androgen-mediated processes in the body.

[0008] The prostate gland depends on androgen hormone action for its devel-opment and growth, and the development of human benign prostatic hyperplasia (BPH) clearly requires a combination of testicular androgens during the aging process. However, testosterone is not the major androgen responsible for growth of the prostate. The principal prostatic androgen is 5a-dihydrotestosterone (5a-DHT), as evidenced by current treatments of prostatic cancer are directed toward reducing 5a-DHT with 5a-reductase inhibitors. Although not elevated in human BPH, 5a-DHT levels in the prostate remain at a normal level with aging, despite a decrease in the plasma testosterone. Testosterone is converted to 5a-DHT by 5a-reductase in prostatic stromal and basal cells. 5a-DHT is primarily responsible for prostate development and the pathogenesis of BPH. Inhibitors of 5a-reductase re-duce prostate size by 20% to 30%. This reduction in glandular tissue is achieved by the induction of apoptosis, which is histologically manifested by ductal atro-phy. 5a-reductase occurs as 2 isoforms, type 1 and type 2, with the prostate ex-pressing predominantly the type-2 isoform, and the liver and skin expressing pri-marily the type-1 isoform. Patients have been identified with deficiencies in the type-2 5a-reductase, but not type 1. Knockout mice with the type-2 5a-reductase null-mutation demonstrate a phenotype similar to that seen in men with 5a-reductase deficiency. Type-1 5a-reductase knockout male mice are phenotypically normal with respect to reproductive function. Enzymatic activity for 5a-reductase or immunohistochemical detection has been noted in other genitourinary tissues, such as the epididymis, testes, gubemaculum, and corporal cavemosal tissue.

[0009] Quantitatively, women secrete greater amounts of androgen than of es-trogen. The major circulating steroids generally classified as androgens include dehydroepiandrosterone sulphate (DHEAS), dehydroepiandrosterone (DHEA), androstenedione (A), testosterone (T), and 5a-DHT in descending order of serum concentration, though only the latter two bind the androgen receptor to a signifi-cant degree. The other three steroids are better considered as pro-androgens.
5a-DHT is primarily a peripheral product of testosterone metabolism. Testosterone circulates both in its free form, and bound to protein including albumin and sex steroid hormone-binding globulin (SHBG), the levels of which are an important determinant of free testosterone concentration. The postmenopausal ovary is an androgen-secreting organ and the levels of testosterone are not directly influenced by the menopausal transition or the occurrence of menopause.

[0010] The work of some research has focused on the development of ster-oidal compounds for the treatment of androgen dependent diseases such as: hir-sutism, androgenic alopecia, benign prostatic hyperplasia (BPH) and prostate can-cer. 5a-DHT has been implicated as a causative factor in the progression of these diseases, largely through the clinical evaluation of males who are genetically defi-cient of steroid 5a-reductase enzyme. As a result of such studies, the inhibition of this enzyme has become a pharmacological strategy for the design and synthesis of new antiandrogenic drugs. However, it is unclear whether inhibition of 5a-reductase will have a deleterious impact on the system, as evidenced by contrain-dications arising from reported side effects of conventional treatments using 5a-reducatse inhibitors. The development of different strategies that target the inhibi-tion of 5a-DHT effects would be a major advance in the therapy of androgen-mediated conditions.

SUMMARY

[0011] The present invention is a method of preventing or ameliorating a neu-ropsychiatric or neurodegenerative disease or disorder in a subject. The method includes administering a composition comprising equol in an amount sufficient to prevent or ameliorate the neuropsychiatric or neurodegenerative disease or disor-der.

[0012] The equol may be a racemic mixture of R-equol and S-equol. Prefera-bly, the equol is enantiomerically enriched with R-equol. Alternatively, the equol may be enantiomerically enriched with S-equol.

[0013] The neuropsychiatric or neurodegenerative disease or disorder may be depression, anxiety, bipolar disorder, obsessive-compulsive disorder, hyperactive disorder, weight gain or obesity. The neuropsychiatric or neurodegenerative dis-ease or disorder may be Alzheimer's disease or Parkinson's disease. The neuro-psychiatric or neurodegenerative diseases or disorder may be a peri- or postmeno-pausal symptom.

[0014] The composition may further comprise an excipient. Preferably, the composition is in an oral formulation selected from the group consisting of a tab-let, capsule, powder, trouche, buccal tablet, and sublingual tablet. The composi-tion may be an oral formulation comprising at least about 0.25 mg of equol.
The composition may be a food product and a beverage. The composition may be a delayed or a sustained release formulation.

[0015] Preferably, the equol is in an amount sufficient to bind free 5a-dihydrotestosterone and inhibit its binding with androgen receptors.
Preferably, the equol is in an amount sufficient to bind free 5a-dihydrotestosterone and inhibit its binding with androgen receptors, and sufficient to bind estrogen receptor sub-types.

[0016] In another embodiment, the present invention is a method of reducing obesity in a subject. The method includes administering a composition comprising a therapeutically effective amount of equol.

[0017] In another embodiment, the present invention is a method of reducing depression in a subject. The method includes administering a composition com-prising a therapeutically effective amount of equol.
[00181 In yet another embodiment, the present invention is a method of reduc-ing anxiety in a subject. The method includes administering a composition com-prising a therapeutically effective amount of equol.
[00191 In further embodiment, the present invention is a method of providing a personalized treatment of a neuropsychiatric or neurodegenerative disease or dis-order in a subject. The method includes assessing a condition of the emotional, mental or endocrine health of the patient; assessing an equol-producer status of a patient; and determining an optimally beneficial course of treatment, selected from the group consisting of a) mode of administration, b) a dose amount, and c) a dose interval.

BRIEF DESCRIPTION OF THE DRAWINGS

[0020] FIG. 1 shows the chemical structures of S-equol and R-equol enanti-omers.

[0021] FIG. 2A shows an appearance/disappearance plot of R-equol in plasma after oral administration of R-equol to a healthy adult.
[0022] FIG. 2B shows an appearance/disappearance plot of S-equol and R-Equol in plasma (mean levels) after oral administration of S-equol or R-equol in healthy human adults (from: K.D. Setchell et al., Am J. Clin. Nutr., 81: 1072-1091, 2005).
[0023] FIG. 3 shows a mass chromatogram of the elution of the equol enanti-omers from a sample of urine from an adult consuming soy food, compared against pure enantiomeric standards that had been characterized by optical dichro-ism.
[0024] FIG. 4 shows the GC-MS analysis of the trimethylsilyl ether derivative of synthesized product.
[0025] FIG. 5 shows a mass chromatogram of a chiral separation of S-equol and R-equol from a racemic mixture.
[0026] FIG. 6A shows prostate weight for in intact male rats subcutaneously injected with DMSO or equol.
[0027] FIG. 6B shows lutenizing hormone (LH) for in intact male rats subcu-taneously injected with DMSO or equol.
[0028] FIG. 7 shows a distinct peak in [3H] DHT+equol but not [3H] DHT
alone.
[0029] FIG. 8A shows two distinct peaks in [3H]-DHT+equol incubated with prostate (A), while, [0030] FIG. 8B shows only a single peak is present in [3H]-DHT incubated with prostate (B).
[0031] FIG. 9 shows the specific binding of equol to [3H]-DHT.
[0032] FIG. 10A shows prostate weight in gonadectomized (GDX) male rats sc injected with DMSO, 5a-DHT, equol, or both 5a-DHT and equol.
[0033] FIG. I OB shows plasma LH in gonadectomized (GDX) male rats sc in-jected with DMSO, 5a-DHT, equol, or both 5a-DHT and equol.
[0034] FIG. 11 shows plasma 5a-DHT levels in rats treated with DMSO, DHTP, equol, or both DHTP and equol.

[0035] FIG. 12 shows the histological effects of equol in the prostate gland of GDX (A-D) and intact (E & F) rats treated with either Trent: DMSO (A & E), equol (B & F), DHT (C), or DHT plus equol (D).
[0036] FIG. 13 shows the histological effects of equol on the epididymis of in-tact rats treated with DMSO (A) or equol (B).
[0037] FIG. 14 shows body weight in male rats fed either an isoflavone-rich (Phyto-600) or a phytoestrogen-free (Phyto-Free) diet.
[0038] FIG. 15 shows the white adipose tissue mass in male rats fed either a Phyto-600 or Phyto-Free diet.
[0039] FIGS. 16A and 16B show food and water intake in male rats fed a Phyto-600 or a Phyto-Free diet, respectively.
[0040] FIGS. 17A and 17B show plasma leptin and insulin levels from male rats fed a Phyto-600 or a Phyto-Free diet, respectively.
[0041] FIG. 18 shows serum glucose levels from male rats (non-fasting) fed either a Phyto-600 or Phyto-Free diet.
[0042] FIG. 19 shows thyroid (T3) serum levels in male rats fed either a Phyto-600 or Phyto-Free diet.
[0043] FIG. 20 shows body weights of female rats fed either a Phyto-600 or Phyto-Free diet.
[0044] FIG. 21 shows the white adipose tissue mass from female rats fed either a Phyto-600 or a Phyto-Free diet.
[0045] FIG. 22 shows serum glucose levels from female rats fed either a Phyto-600 or Phyto-Free diet.
[0046] FIG. 23 shows serum T3 levels from female rats fed either a Phyto-600 or Pllyto-Free diet.
[0047] _ FIG. 24 shows the body weights of female rats fed either a Phyto-600 or a Phyto-Free diet after 50 days of age.
[0048] FIG. 25 shows the white adipose tissue mass of female rats fed either a Phyto-600 or al Phyto-Free diet after 50 days of age.
[0049] FIG. 26 shows the serum leptin levels of female rats fed either a Phyto-600 or a Phyto-Free diet after 50 days of age.

[0050] FIG. 27 shows the serum insulin levels of female rats fed either a Phyto-600 or a Phyto-tree diet after 50 days of age.
[0051] FIG. 28 shows body weights of OVX rats fed either a Phyto-600 (black bars) or a Phyto-Free (white bars) diet after and placed on a behavioral estrus in-duction regimem.
[0052] FIG. 29 shows the white adipose tissue mass of OVX rats fed either a Phyto-600 or 4 Phyto-Free diet.
[0053] FIG. 30 shows the serum leptin levels of OVX rats fed either a Phyto-600 or a Phyto-Free diet.
[0054] FIG. 31 shows the body weights of 112-day-old male rats fed AIN-76, Phyto-Free, Phyto-200, or Phyto-600 diets.
[0055] FIG. 32 shows the body weights of 279-day-old male rats fed AIN-76, Phyto-Free, Phyto-200, or Phyto-600 diets.
[0056] FIG. 33 shows the body weights of 350-day-old male rats fed AIN-76, Phyto-Free, Phyto-200, or Phyto-600 diets.
[0057] FIG. 34 shows the adipose tissue mass from 350-day-old male rats fed AIN-76, Phyto-Free, Phyto-200, or Phyto-600 diets.
[0058] FIG. 35 shows serum insulin levels in 350-day-old male rats fed AIN-76, Phyto-Free, Phyto-200, or Phyto-600 diets.
[0059] FIG. 36 shows serum leptin levels in 350-day-old male rats fed AIN-76, Phyto-Free, Phyto-200, or Phyto-600 diets.
[0060] FIG. 37 shows body weights of 112-day-old female rats fed AIN-76, Phyto-Free, Phyto-200, or Phyto-600 diets.
[0061] FIG. 38 shows body weights of 279-day-old female rats fed AIN-76, Phyto-Free, Phyto-200, or Phyto-600 diets.
[0062] FIG. 39 shows body weights of 145-day-old male Noble rats fed Phyto-Free or Phyto-600 diets.
[0063] FIG. 40 shows white adipose tissue mass from 145-day-old male Noble rats fed Phyto-Free or Phyto-600 diets.
[0064] FIG. 41 shows body weights of 145-day-old female Noble rats fed Phyto-Free or Phyto-600 diets.

[0065] FIG. 42 shows white adipose tissue mass from 145-day-old female No-ble rats fed Phyto-Free or Phyto-600 diets.
[0066] FIG. 43 shows baseline body weights of three groups of rats on a Phyto-Free diet prior to receiving equol injections.
[0067] FIG. 44 shows body weights of three groups of rats after 21 days on a Phyto-Free diet prior to receiving equol or vehicle injections.
[0068] FIG. 45 shows body weights of three groups of rats on a Phyto-Free diet 7 days after receiving equol or vehicle injections.
[0069] FIG. 46 shows body weights of three groups of rats on a Phyto-Free diet 15 days after receiving equol or vehicle injections.
[0070] FIG. 47 shows body weights of three groups of rats on a Phyto-Free diet 22 days after receiving equol or vehicle injections.
[0071] FIG. 48 shows body weights of three groups of rats on a Phyto-Free diet 28 days after receiving equol or vehicle injections.
[0072] FIG. 49 shows adipose tissue mass from three groups of rats on a Phyto-Free diet 28 days after receiving equol or vehicle injections.
[0073] FIG. 50 shows testes weight from three groups of rats on a Phyto-Free diet 28 days after receiving equol or vehicle injections.
[0074] FIG. 51 shows number elevated-plus maze anxiety-related behavior (entries into open arms) of 300-day-old male rats fed 4 different diets.
[0075] FIG. 52 shows elevated-plus maze anxiety-related behavior (time in open arms) of 300-day-old male rats fed 4 different diets.
[0076] FIG. 53 shows elevated-plus maze anxiety-related behavior (entries into open arms) of 330-day-old female rats fed 4 different diets.
[0077] FIG. 54 shows elevated-plus maze anxiety-related behavior (time in open arms) of 330-old female rats fed 4 different diets.
[0078] FIG. 55 shows serum isoflavone levels in 300-day-old male rats fed 4 different diets.
[0079] FIG. 56 shows serum isoflavone levels in 330-day-old female rats fed 4 different diets.

[0080] FIG. 57 shows the observed change in BMI for individuals after 5 weeks of strict adherence to a diet containing isoflavones.
[0081] FIG 58 is a graph of the total distance traveled in meters before the equol injections (A) and after the equol injections (B) in Phyto-free fed mid-aged female rats compared to animals fed a Phyto-600 diet in the Porsolt swim test.
[0082] FIG. 59 is a graph of the overall average speed (in meters per second) before the equol injections (A) and after the equol injections (B) in Phyto-free fed mid-aged female rats compared to animals fed a Phyto-600 diet in the Porsolt swim test.
[0083] FIG. 60 is a graph of the total time mobile in seconds before the equol injections (A) and after the equol injections (B) in Phyto-free fed mid-aged female rats compared to animals fed a Phyto-600 diet in the Porsolt swim test.
[0084] FIG. 61 is a graph of the total number of dives before the equol injec-tions (A) and after the equol injections (B) in Phyto-free fed mid-aged female rats compared to animals fed a Phyto-600 diet in the Porsolt swim test.
[0085] FIG. 62 is a graph of the total time immobile in seconds before the equol injections (A) and after the equol injections (B) in Phyto-free fed mid-aged female rats compared to animals fed a Phyto-600 diet in the Porsolt swim test.
[0086] FIG. 63 is a graph of the total number of boli excreted before the equol injections (A) and after the equol injections (B) in Phyto-free fed mid-aged female -rats compared to animals fed a Phyto-600 diet in the Porsolt swim test.
[0087] FIG. 64 is a graph of the body weight loss in equol-treated adult male rats compared to Phyto-(Free) fed control animals, n = 8 per treatment group.
[0088] FIG. 65 is a graph of the number of entries into the open arms of the elevated plus maze (EPM).
[0089] FIG. 66 is a graph of the time spent in the open arms of the EPM.
[0090] FIGA7 is an illustration of the hormone sensitive hypothalamic regions of the brain in adult male rats after being treated with equol for 25 consecutive days.
[0091] FIG. 68 is a graph of the number of entries into the open arms of the EPM.

[0092) FIG. 69 is another graph of the time spent in the open arms of the EPM
of male rats treated during prenatal and early postnatal development with equol coinpared to genistein or control animals.

DETAILED DESCRIPTION OF THE DRAWINGS AND THE PRES-ENTLY PREFERRED EMBODIMENTS

[0093] Despite the recent gains in understanding the pharmacology of equol as it pertains to estrogen actions, our research showing potent antiandrogen effects of equol is unique and novel and opens new approaches to preventing or treating an-drogen-related conditions. Binding or sequestering 5a-DHT would provide a means for inhibiting its effect on 5 a-DHT- sensitive tissues. There is no known ligand that is specific for 5a-DHT, but such an agent would have distinct advan-tages over non-discriminatory compounds that target the androgen receptor di-rectly or the enzymes involved in androgen synthesis.
[0094] The invention is based on the unexpected discovery by the inventor that equol may be used to prevent and/or treat neuropsychiatric disorders, such as depression, anxiety; and neurodegenerative disorders, such as Alzheimer's dis-ease, Parkinson's disease or minor dementia. The inventor further noted that equol may influence motivation for and regulation of feeding and weight control.
[0095] These findings have important ramifications in health and disease and indicate potential broad and important usage for equol in the treatment of andro-gen mediated pathologies. Without intending to be bound by a particular mecha-nism it is believed that equol can act as an anti-androgen. The anti-androgenic properties of equol are unique in that equol does not bind the androgen receptor (AR) but specifically binds 5a-dihydrotestosterone (5a-DHT) with high affinity and thereby prevents 5a-DHT from binding the AR. Furtherinore, both the R- and S-enantiomers of equol may specifically bind 5a-DHT, sequester 5a-DHT from the AR and block 5a-DHT's actions in physiological processes in vivo. Racemic equol, which constitutes R-equol and S-equol and R-equol or S-equol alone, selec-tively bind 5a-DHT.

[0096] In mammals, there are two principal androgens, testosterone and its 5a-reduced metabolite, 5a-DHT. 5a-DHT is recognized as the most potent androgen in the mammalian body. The AR, which is encoded by a single-copy gene located on the human X-chromosome specifically mediates the actions of androgens. Al-though both testosterone and 5a-DHT bind the AR, certain tissues (i.e.
prostate gland, hair follicles, etc.) that are only slightly influenced by testosterone are greatly influenced by 5a-DHT. Furthermore, 5a-DHT has been implicated in a number of diseases and disorders. Because, as mentioned above, equol specifi-cally binds and prevents the actions of 5a-DHT, there is an indication for a broad and important usage for equol in the treatment of androgen-mediated pathologies.
Specifically, the inventors discovered that equol may be used to prevent or treat neuropsychiatric and neurodegenerative diseases or disorders.
[0097] Equol has a structure similar to the steroidal estrogen estradiol.
Equol is unique among the isoflavones in that it possesses a chiral center and as such ex-ists as two distinct enantiomeric forms, the R- and S-enantiomers. All previous studies on equol appear to have been conducted with the racemic form of equol.
There has in general been a lack of appreciation that two forms of equol exist and to our knowledge no previous study has reported on the specific actions or activity of the individual enantiomers R- and S-equol specifically bind 5a-DHT. Equol racemic, R-equol or S-equol does not bind the androgen receptor. R-equol does not bind the estrogen receptor system. S-equol only binds ERP (with an affinity approximately 5-times less than 17(3-estradiol). Thus, R- and S-equol have SERM-like properties along with having the capability to selectively bind the most potent circulating androgen, 5a-DHT.
[0098] Most of the interest in soy and its constituent isoflavone, genistein, and to a lesser extent daidzein, has focused on either their estrogenic actions, or other non-hormonal actions such as their effects on enzymes, growtll factors or cyto-kines, or their antioxidant actions. Never previously has there been any discussion of the potential antiandrogen actions for isoflavones and rarely is the enantiomeric forms of equol even mentioned. The present invention addresses the effects of the enantiomeric forms of equol, and specifically, the ability of both S-equol, the natu-ral metabolite of daidzein, and R-equol antagonize the actions of the potent andro-gen dihydrotestosterone, 5a-DHT. Also, without wishing to be bound by a par-ticular theory, it is currently believed that equol plays a positive role acting as a estrogen-like molecule via the estrogen receptor subtypes. Such effects open up novel possibilities for dietary, nutraceutical, and pharmacological approaches to prevention and treatment of disease where 5a-DHT plays a detrimental role.
This includes but is not restricted to prostate cancer, skin diseases, hair loss, neuropsy-chiatric diseases or disorders, and neurodegenerative diseases. Additionally, the estrogenic actions of S-equol may also be of benefit in treating or preventing pros-tate cancer because the combined actions of equol acting at the estrogen receptor level and as an antiandrogen.

Equol Binds with 5a-DHT
[0099] Equol (7-hydroxy-3(4'hydroxyphenyl)-chroman) represents the major metabolite of phytoestrogens daidzin and daidzein, isoflavones found abundantly in soybeans and soy-foods, and is an important biologically active molecule.
In animals fed a phytoestrogen-rich diet, the major circulating isoflavone is equol, which accounts for 70-90% of the total circulating isoflavone levels. The present invention discloses a novel model of equol's biological properties. In binding studies, equol enantiomers specifically bind 5a-DHT, but not testosterone, DHEA
or estrogen. By doing so, equol sequesters 5a-DHT from the androgen receptor without directly binding the androgen receptor itself. In vivo studies demonstrate that equol treatment of intact male rats significantly decreased prostate and epidi-dymis but not testes weights. In castrated male rats treated with 5a-DHT after administering equol, equol blocked 5a-DHT's trophic effects on the prostate gland and its negative feedback effects on plasma luteinizing hormone (LA) levels.
[00100] While not wishing to be bound by a particular theory of the mecha-nism of action, it is currently believed that equol can act as an anti-androgen, by specifically binding 5a-DHT and preventing 5a-DHT from binding to the andro-gen receptor (AR) without itself binding the AR. It also has been shown that 5a-DHT that has already been bound to the AR will not be competitively bound by enantiomeric equol. Therefore, one embodiment of the present invention is a method of preventing 5a-DHT binding to the AR by contacting 5a-DHT with equol prior to DHT-AR binding occurs. The enantiomeric equol may be brought into contact with the 5a-DHT in vitr^o or in vivo.. When the 5a-DHT is to be con-tacted in vivo, the equol may be administered by any route that allows absorption of equol to the blood stream. Biologically available 5a-DHT is free and unbound by any native ligand prior to binding with equol.
[00101] Reproductive organs such as the prostate and epididymis are known to be under androgenic control. For example, before puberty, when circulating androgen levels are very low, rats fed a diet containing high levels of soy-derived isoflavones have prostate weights that are not altered by the consumption of this diet. However, after puberty when androgen levels increase, prostate weights are significantly decreased in phytoestrogen-rich-diet fed rats compared to animals fed a phytoestrogen-free diet. As shown herein, equol-treated intact rats display sig-nificant decreases in prostate and epididymis weights (without alterations in testes or pituitary weights during short-term studies). Notably, if the prostate and epidi-dymal values are standardized to body weight (per 100 grams) the ratios are still significantly different between equol-treated and control values. Equol also blocked 5a-DHT's androgenic trophic influence on the prostate and epididymis, without significantly altering testosterone levels.
[00102] 5a-DHT has negative feedback effects on circulating plasma levels of luteinizing hormone (LH). It is believed that equol significantly increases LH
levels by binding 5a-DHT and preventing this feedback effect. For example, equol completely reverses the inhibitory action of 5a-DHT on LH levels in go-nadectomized (GDX) males, whereas 5a-DHT plus equol-treated male rats display LH levels similar to that of control values. These data further suggest that equol may have a specific ability to bind 5a-DHT, presumably in the blood circulation system, and block the hormonal action of 5a-DHT in suppressing LH production or secretion. Therefore an embodiment of the present invention is a method of modulating LH levels in an individual by contacting the 5a-DHT of the individual with enantiomeric equol. The equol can be administered by any route that allows absorption of equol to the blood stream with the amount administered in accor-dance with the nature of the ailment to be treated and size of the individual.

The Structure of Equol [00103] Equol is distinct from most isoflavones in having a chiral center due to the lack of a double bond in the heterocyclic ring. The phytoestrogen isofla-vones from soy (daidzein, glycitein and genistein), clover (formononetin and bio-chanin A), and kudzu, (peurarin), do not have a chiral center. FIG. 1 shows the chemical structures of R-equol and S-equol.
[00104] The R-and S-enantiomers conformationally differ and this is pre-dicted to influence how an equol enantiomer fits into the binding site in the cavity of the dimerized ER complex, and how it binds with 5a-DHT.
[00105] Approximately 50% of equol circulates in the free or unbound form in humans, and this is considerably greater than the proportion of free daidzein (18.7%) or estradiol (4.6%) in plasma. Since it is the unbound fraction that is available for receptor occupancy, and presumably for binding 5a-DHT, this would effectively contribute to enhancing the overall potency of equol.

Compositions Containing Equol [00106] The present invention includes a composition having an at least physiological acceptable quantity of equol that is able to bind and sequester free 5a-DHT (but not testosterone or DHEA) thereby preventing it binding to the an-drogen receptor following administration to an individual thereby having impor-tant ramifications in health and disease and a broad and important use in the treat-ment of androgen-mediated pathologies. Specifically, the compositions compris-ing equol may be used to prevent or treating neuropsychiatric or neurodegenera-tive diseases or disorders.
[00107] Preferably, compositions containing equol (which may be S-equol, R-equol, a racemic equol mixture, or a non-racemic equol mixture), are made for oral consumption.

[00108] Preferably, equol is in an amount sufficient to prevent or treat dis-eases or disorder according to the methods of this invention. Preferably, a thera-peutically effective amount of equol is used in the methods of this invention.
The term "therapeutically effective amount" refers to the amount or quantity of equol (S-equol, R-equol, a racemic equol mixture, or a non-racemic equol mixture), which is sufficient to elicit the required or desired prophylactic or therapeutic re-sponse, or in other words, the amount which is sufficient to elicit an appreciable biological response when administered to a subject. For example, a therapeuti-cally effective amount of equol may reduce anxiety or depression in a subject.
[00109] The composition or a product containing the composition may be a marketed or institutional food product, a pharmaceutical (i.e., drug), and an over-the-counter (OTC) medicament (i.e., nutraceutical).
[00110] Preferably, a food composition comprises at least about 0.25 mg, more preferably, the food composition comprises about 1 mg, and preferably up to about 200 mg, enantiomeric equol or equol mixtures, per serving. Other amounts of the equol in a food composition are also contemplated.
[00111] Preferably, an orally-administered medicament comprises at least about 0.25 mg, more preferably about 1 mg, and preferably up to about 200 mg, enantiomeric equol or equol mixture, per dose. Other amounts of the equol in a medicament composition are also contemplated.
[00112] Preferably, a product for topical application can comprise at least about 0.1 %, and up to about 10%, by weight S-equol, or R-equol, or enantiomeric mixtures. Other concentrations of equol are also contemplated for topical products containing equol.
[00113] A composition of the present invention may also include other cos-metic and pharmaceutical actives and excipients. Such suitable cosmetic and pharmaceutical agents include, but are not limited to, antifungals, vitamins, anti-inflammatory agents, antimicrobials, analgesics, nitric oxide synthase inhibitors, insect repellents, self-tanning agents, surfactants, moisturizers, stabilizes, pre-servatives, antiseptics, thickeners, lubricants, humectants, chelating agents, skin penetration enhancers, emollients, fragrances and colorants.

[00114] An enantiomeric equol can also be an enantiomeric equol conjugate, conjugated at the C-4' or the C-7 position with a conjugate selected from the group consisting of glucuronide, sulfate, acetate, propionate, glucoside, acetyl-glucoside, malonyl-glucoside, and mixtures thereof.
[00115] A composition or preparation comprising enantiomeric or mixture of equol, for administering to subjects for the treatment and/or prevention of, or for reducing the predisposition to, androgen-related diseases and conditions related thereto, may also comprise one or more pharmaceutically acceptable adjuvants, carriers and/or excipients. Pharmaceutically acceptable adjuvants, carriers and/or excipients are well known in the art, for example as described in the Handbook of Pharmaceutical Excipients, second edition, American Pharmaceutical Association, 1994 (incorporated herein by reference).
[00116] In one embodiment, the composition of the invention comprises a non-racemic mixture of S-equol and R-equol, having an EE for S-equol of more than 0% and less than 90%. A composition that has an EE of 0% is a 50:50 race-mic mixture of the two enantiomers. The composition can be made directly from a racemic mixture, by an incomplete separation and removal of either the R-equol or S-equol enantiomer from the racemic mixture. The composition can also be made by combining a first equol component comprising a mixture (either a non-racemic or racemic mixture) of equol, with a second component comprising a composition consisting essentially of S-equol or R-equol. This produces a non-racemic composition that has an excess of S-equol or R-equol. Depending upon the specific benefit or indication for the R-equol component and the S-equol com-ponent in a composition, a composition can be prepared comprising S-equol and R-equol at a ratio of S-equol to R-equol preferably from greater than about 50:50 to about 99.5: 1, more preferably about 5 1:49 to about 99:1, and preferably from less than about 50:50 to about 1:99.5, more preferably about 49:51 to about 1:99.
[00117] The composition may be administered in the form of tablets, cap-sules, powders for reconstitution, syrups, food (such as food bars, biscuits, snack foods other standard food forms well known in the art), or in drink formulations.
Drinks may contain flavoring, buffers and the like.

[00118] Compositions suitable for oral administration may be presented in discrete units, such as capsules, cachets, lozenges, trouche, tablets, buccal tablets, sublingual tablet, each containing a predetermined amount of the extract; as a powder or granules; as a solution or a suspension in an aqueous or non-aqueous liquid; or as an oil-in-water or water-in-oil emulsion.
[00119] The composition typically does not comprise a significant amount of any other androgen-receptor binding compound.

Identifying Equol Producers and Non-Equol Producers [00120] Equol is formed following the hydrolysis of the glycoside conju-gates of daidzein from soy, and the methoxylated isoflavone formononetin, or its glycosidic conjugates found in clever. Once formed, equol appears to be metab-olically inert, undergoing no further biotransformation, save phase II
metabolism or a minor degree of additional hydroxylation in the liver. As with daidzein and genistein, the predominant phase II reactions are glucuronidation and, to a minor extent, sulfation. Following the original discovery that equol's presence in urine was a function of soy food ingestion, it was observed that approximately 50-70%
of the adult population did not excrete equol urine even when challenged daily with soy foods, for reasons that are unclear. Furthermore, even when the pure isoflavone compounds are administered, thereby removing any influence of the food matrix, it has been shown that many people do not convert daidzein to equol.
This phenomenon has led to the terminology of a person being an `equol-producer' or `non-equol producer' (or `poor equol-producer') to describe these two distinct populations.

[00121] Cut-off values have been empirically derived permitting assignment of individuals to either of these categories. People who have plasma equol con-centrations of less than 10 ng/mL (40 nmol/L) can be classified as `non-equol pro-ducers' and where levels are above 10 ng/mL (40 nmol/L) this defines `equol pro-ducers'. This distinction can also be derived from the levels in urine, an equol producer being someone excreting greater than 1000 nmol/L. Although the excre-tion of equol is highly variable among individuals there is a large demarcation be-tween those that can produce equol and those that cannot, consistent with a precur-sor-product relationship in enzyme kinetics catalyzing the reaction. There is con-sequently an inverse relationship between urinary daidzein and equol levels, and thus far no significant gender differences have been defined.

Preparation and Isolation of Equol Enantiomers [00122] Enantiomeric equol can be prepared per se or as the racemic mix-ture. Chemical synthesis routes can be used to produce the racemic mixture in good yields. In a typical synthesis process, standard chemical treatments are used to hydrogenate the double-bond of the heterocyclic ring and to remove the car-bonyl at position C-3. Typical starting materials are isoflavones such as daidzein, genistein, glycitein, peurarin, formononetin and biochanin A and their glucoside conjugates. Any conjugated form would be reduced to its aglycon by hydrolysis.
Suitable solvents for the reaction include organic acids such as glacial acetic acid, lower alcohols such as isopropanol, and mixtures thereof. Reduction catalysts typically employed include Palladium, such as 10% Pd on charcoal. Reactions can run at temperatures from ambient to 60 C., with pressures ranging from slightly above ambient, up to 200 psig (14 atm. gauge), and with reaction times of up to 30 hours or more.

[00123] After reaction completion, the catalyst is removed and any filtrate evaporated. The crude residue is purified, typically by chromatography employing a silica gel column, with an eluent comprising C2-C4 alcohols, C3-C7 alkanes, and mixtures thereof. The purified residue can be crystallized from n-hexane to produce ( )equol as a pure product, typically of at least 99%, with a yield typically of at least 75%. The equol crystallized product is colorless, not hygroscopic, and stable in air, and does not decompose during the final filtration procedure.

Method for the Isolation of the Individual R- and S-Enantiomers from Racemic Equol [00124] A racemic mixture of equol can be separated into its two distinct enantiomers using a chiral-phase column with a mobile phase comprising a C4-C8 alkyl and a C2-C4 alcohol. A typical example of a chiral-phase column is a Chiralcel OD column or OJ column, supplied by Daicel Chemical Industries Ltd.
A preferred example of a mobile phase comprises 70% hexane and 30% ethanol.
After a first period of time from passing the racemic mixture into the inlet, the time period depending upon the type of column, type of eluent, eluent flow rate, temperature, and mass of the racemic mixture, a first effluent is collected from an outlet of the HPLC column. The first eluent is typically S-equol. After a second period of time from passing the racemic mixture into the inlet, the second eluent R-equol is obtained. The elution of an equol enantiomer from the column can be detected by UV absorbance at 260-280 nm or by a more specific detection system such as a mass spectrometer and monitoring of ions specific to equol.

Biological Production of S-Equol [00125] S-equol can be produced biologically in bulk using conventional food technology. A base solution media, food product or plant extract can be pro-vided that comprises daidzein or another related isoflavone from which daidzein can be derived. The daidzein or other isoflavone can be converted to S-equol by a standard bacterial or enzyme fermentation process, to provide a bulk solution, food product or plant extract that comprises S-equol.
[001261 Conversion of daidzein to equol involves three major steps: 1) hy-drolysis of any glucoside conjugate group, 2) conversion of the isoflavone agly-cons to a dihydro-intermediate, and 3) conversion of the dihydro-intermediate to equol. The metabolic pathway and enzymes for each of the three steps required may not necessarily be present in one bacterium. Anecdotal evidence from human studies suggests that there may be one or more bacteria that act in conjunction to perform these reactions, as evidenced from the fact that often dihydrodaidzein can be present in significant amounts in plasma and urine yet equol may be low or barely detectable. Although equol may be produced from daidzein by a single or-ganism it is believed that better or more efficient conversion can be achieved when using a mixture of bacterial species, each with its own metabolic profile.
Important conditions for effective conversion to S-equol include the selection of the bacterial organism or mixture of organisms, the temperature of incubation, and the amount of oxygen available to the organisms. These conditions can be optimized by tech-niques well known to persons skilled in this art. The organisms used to effect this change can be inactivated by standard techniques used in the food industry or, al-ternately, allowed to remain in an active state in the product.
[00127] Typically, one or more bacterial strains are required to convert the daidzein (or other related isoflavone) through intermediate products to S-equol, which generally involves one or more of the three major reactions: the conversion of isoflavone glycone to aglycon isoflavone; the conversion of aglycon isoflavone to dihydro isoflavone; and the conversion of dihydro isoflavone to the product, equol. For example, a mixed culture of organisms isolated from equine feces or a mixed culture of organisms derived from the gastrointestinal tract of a person known to an `equol producer' can convert, as they do in vivo, the glycone daidzein to the final product S-equol.
[00128] Typical bacterial strains that can convert a glycone to an aglycon (such as daidzein to daidzein) include Enterococcus faecalis, a Lactobacillus plan-tarum, Listeria welshimeri, a mixed culture of organisms isolated from the intesti-nal tract of an `equol producing' mammal, Bacteriodes fragilis, Bifidobacterium lactis, Eubactria limosum, Lactobacillus casei, Lactobacillus acidophilous, Lacto-bacillus delbrueckii, Lactobacillus paracasei, Listeria monocytogenes, Micrococ-cus luteus, Proprionobacterium freudenreichii and Sacharomyces boulardii, and mixtures thereof.
[00129] Typical bacterial strains that can convert an aglycon to equol (such as daidzein to S-equol) include Proprionobacteria freundenreichii, a mixed culture containing: Bifidobacterium lactis, Lactobacillus acidophilus, Lactococcus lactis, Enterococcus faecium, Lactobacillus casei and Lactobacillus salivarius; and a mixed culture of organisms isolated from the intestinal tract of an `equol produc-ing' mammal.

[00130] The time required for bacterial conversion of the glucosides to agly-cons, or the aglycons to the equol product, will depend upon bacteria-related fac-tors, particularly concentration, the availability of oxygen, and the temperature and pH of the incubating system. In most instances it is possible to achieve substan-tially complete conversion within 24 hours.
[00131] The pH range for bacterial conversion of the isoflavone glucosides to aglycon isoflavones is from about 3 to about 9. The optimum pH depends pri-marily upon the type of bacteria used, and should be selected accordingly.
[00132] The time required for enzymatic conversion of the glucosides to aglycons, and aglycons to the equol product, depends upon enzyme-related fac-tors, particularly concentration, and the temperature and pH of the system. In most instances it is possible to achieve substantially complete conversion within 24 hours, more preferably within about 2 hours, and most preferably within about 1 hour.
[00133] S-equol produced in bulk can be separated from the resulting bulk solution of a bacterial production of S-equol, by methods well known in the art, including crystallization, solvent extraction, distillation, and precipita-tion/filtration. The resulting bulk solution can contain unreacted daidzein or other related isoflavone used, by-products,'and any reactants. Such methods can include the use of a reverse-phase or straight-phase liquid chromatography column and these can be combined with chiral-phase chromatography.
[001341 A typical method of removing S-equol from a bulk solution or solid phase is by extraction. An extractant solution is added to the solution or solid phase containing the S-equol. Typically the extractant is a low molecular weight alcohol such as methanol, ethanol, isopropyl alcohol, or propyl alcohol, or an aqueous solution having a pH in the range from 3.5 to 5.5. Typically, if the aque-ous alcohol method is being used, sufficient alcohol is added to bring the alco-hol/water ratio to between a minimum of 40:60 and a maximum of 95:5. More typically, the ratio is at least 60:40, and even more typically a ratio between 65:35 and 90:10.
[00135] If an aqueous acid extraction method is used an aqueous acid solu-tion is prepared with the pH adjusted to about 3.5 to about 5.5, and more prefera-bly within the pH range of about4.0 to about 5Ø Sufficient water is added to make a dilute liquid with a sufficiently low viscosity to permit separation of solids from liquids by centrifugation or filtration.
[00136] The liquid, from which insoluble solid matter has been removed, is concentrated by conventional methods for removing liquids. Methods used typi-cally include, but are not limited to, removal of solvent by evaporation, preferably under reduced pressure. The residual liquid is concentrated to at least about 15%
solids, and up to about 55% solids, more typically to between 30% and 50% sol-ids. The concentrate is then diluted with water to reduce the solids content and increase the water to alcohol ratio. The amount of water added can be varied over a wide range, though a final solid content between 6% and 15%, and more typi-cally about 13%, is preferred. The pH of the mixture is adjusted between about pH 3.0 and about pH 6.5, with a preferred value between about pH 4.0 and about pH 5Ø Typically the temperature is between about 20 C. to about 10 C., and more typically about 5 C. to 7 C.
[00137] The solid material is then separated from the liquid by standard separation techniques (centrifugation or filtration) and yields an equol-rich solid material.

[00138] The equol-rich material can optionally be purified, typically by chromatography employing a silica gel column, with an eluent comprising C2-C4 alcohols, C3-C7 alkanes, and mixtures thereof. The purified residue can be crys-tallized from n-hexane to produce S-equol as a pure product, typically of at least 99%, with a yield typically of at least 75%. The equol crystallized product is col-orless, not hygroscopic, and stable in air, and does not decompose during the final filtration procedure.

[00139] The S-equol product can be authenticated by GC-MS analysis of the trimethylsilyl ether or tert-butyldimethylsilyl ether derivative, or some other ap-propriate volatile derivative of synthesized product as a single pure peak and a mass spectrum that is consistent with the published electron ionization spectrum of the trimethylsilyl (TMS) ether derivative of authentic equol. Confirmation of the product can also be established by direct mass spectrometry using electrospray ionization after introducing the sample into the instrument via an HPLC chiral-phase column.

Treatment of Disease by AdministeringS-Equol, R-Equol, and Mixtures [00140] This present invention provides a means for an individual subject to overcome the problem of not being able to produce equol in vivo, or to supply R-equol in particular, by providing delivery of equol enantiomers, the S-equol or R-equol, or non-racemic mixtures of S-equol and R-equol directly, circumventing the need for intestinal bacteria for its production or for the need to consume soy foods with equol's precursor isoflavones. The delivery of S-equol can also supplement the in vivo production of S-equol in `equol-producers', as well as in `non-equol producers'.
[00141] Supplementing the diet of an equol producer with an equol enanti-omer or mixture, may provide benefits when the ordinary level of S-equol pro-duced by the equol producer is inadequate because of 1) insufficient consumption of isoflavones to produce equol, 2) antibiotic use that ablates the activity of intes-tinal bacteria to make equol from precursor isoflavones, or 3) other health factors that impact the level of equol production, e.g. short bowel syndrome or surgical construction of an intestinal stoma such as ileostomy. In addition, a supplemental level of equol is believed to provide enhanced effect on the health and well-being of the person.
[00142] This invention provides a method for delivering S-equol, R-equol, racemic equol, or non-racemic mixtures of equol, in sufficient amounts to have health benefits toward androgen-related diseases and conditions associated therewith. The anti-androgenic activity of equol can affect a number of tissues throughout the body. In particular, the blocking of androgenic activity of 5a-DHT
may be beneficial for the treatment and prevention of (A) growth of the prostate gland with aging, benign prostatic hyperplasia (BPH) and prostate cancer, (B) fe-male- and male-pattern baldness, (C) facial and body hair growth (hirsutism), skin health (acne, anti-aging and anti-photo aging), skin integrity (collagen and elastin robustness); (D) body weight gain (and loss), reduction in adipose tissue deposi-tion and metabolism of lipids, as well as general regulatory behaviors and effects, such as food and water intake, blood pressure changes, thyroid, glucose, leptin, insulin and the influence on the immune system; and (E) Alzheimer's disease and emotional, mental health issues, such as, mood, depression, anxiety and learning and memory by reducing the 5a-steroid metabolites (covering androgens and pro-gesterone) that are potent modulators of the GABAA receptor in the brain that in-fluences all of the brain characteristics above.
[00143] Typically, the amount of composition comprising the equol is ad-ministered in an amount sufficient to produce a transient level of enantiomeric equol in the blood plasma of the mammal of at least 5 nanograms per milliliter (ng/mL), more typically at least 10 ng/mL or greater, or transient levels of enanti-omeric equol in urine of greater than 1000 nmol/L. Preferably, the composition is administered orally in a dose amount of at least about 0.25 mg, more preferably about 1 mg, more preferably of at least about 5 mg, and of up to about 200 mg, more preferably, up to about 50 mg; of enantiomeric equol. A typical level of bioavailability of R-Equol in plasma after oral administration of 20 mg of R-equol enantiomer to a healthy adult is shown in the appearance/disappearance plots of R-equol in FIGS. 2 A and B.
[00144] The ability to deliver R- and/or S-equol in sufficient amounts is be-lieved to provide several advantages over delivery of a racemic mixture of equol.
First, the potency of R-equol or S-equol alone would typically be at least twice that of the racemic mixture. Second, the human body only produces the S-equol, and therefore, a composition comprising only S-equol represents a "natural"
prod-uct with an ingredient, S-equol, with which the body is familiar. Third, since the R-equol enantiomer has unique properties, a treatment composition comprising only, or substantially only, the R-enantiomer can produce beneficial and/or thera-peutic effects. And fourth, administration of R-equol would supplement any en-dogenous S-equol present and allow for both estrogenic and anti-androgenic ac-tions to occur in the body.
[00145] The invention includes the use of enantiomeric equol to treat and prevent diseases and conditions related to male- and female-pattern baldness.
5a-DHT is a known cause of scalp hair loss. An androgen, specifically the principal circulating androgen, testosterone, is converted to the more potent androgen, 5a-DHT (in the hair follicle), and the hormonal action of 5a-DHT on scalp hair folli-cles cause hair loss. Thus, if the hormonal action of 5a-DHT can be blocked, such as by the use in the present invention of equol to bind 5a-DHT in the circulation (within blood vessels) and within the hair follicle, then scalp hair loss can be de-creased or prevented.
[00146] The invention includes the use of enantiomeric equol to treat and prevent diseases and conditions related facial and body hair. Facial and body hair are regulated by androgens, but oppositely to that of the regulation of scalp hair.
Specifically, the more potent androgen, 5a-DHT, increases facial and body hair.
5a-DHT also increases the production of sebum (oil) from the sebaceous gland, which can contribute to an increase in acne. Thus, the binding of 5a-DHT by equol can cause a decrease in facial and body hair and in secretion of sebum (oil), and a reduction or prevention of acne.
[00147] The invention includes the use of enantiomeric equol to treat and prevent diseases and conditions related to skin effects, skin quality and integrity, skin aging, skin photoaging, and skin pigmentation and lightening. Estrogens, be-fore but especially after menopause, improve skin health by increasing elastin and collagen content to improve skin characteristics or robustness. Also, when skin is damaged by acne or other skin disruptions (scratches, popping pimples or minor cuts, etc.), the repair mechanism is faster and the skin heals better if estrogen or estrogen-like compounds, such as equol, are present. It is believed that an equol enantiomer mixture, and particularly S-equol, is a good stimulator of elastin and collagen and also can protect against photo-aging. Equol's blocking the hormone action of 5a-DHT can decrease sebum oil production from the sebaceous gland, which can decrease or eliminate acne. Since S-equol (though not R-equol) binds estrogen receptor(s) (mainly ER(3), the protective effects of this estrogen-like molecule would stimulate elastin and collagen in the skin. Additionally, since equol is a strong antioxidant, it can protect the skin from aging, including photo-aging.

[00148] The invention includes the use of enantiomeric equol to treat and prevent diseases and conditions related to improved prostate health. The conver-sion of testosterone to the more potent androgen 5a-DHT is the result of the action of the enzyme 5a-reductase within the prostate. 5a-DHT causes benign prostatic hyperplasia (BPH), increases in the prostate weight, and can result in the need for prostatectomies and radiotherapy to treat these conditions. Finally, consumption of soy foods has received increased attention due to their `health benefits' of de-creasing hormone dependent cancers such as prostate and breast cancer. Thus, blockage of 5a-DHT by equol decreases prostate weight in animal models and presumably will block BPH to prevent prostate cancer.
[00149] The invention includes a method of preventing or ameliorating neu-ropsychiatric and neurodegenerative diseases or disorders by administering equol in an amount sufficient to prevent or ameliorate the neuropsychiatric or neurode-generative disease or disorder.

[00150] Neurodegenerative disorders, which are often associated with aging, such as Alzheimer's disease and minor depression, as well as Parkinson's disease, are prevalent in the general population and are expensive to treat. About 4 million people in the U.S. now are thought to have Alzheimer's disease resulting in just over 100 billion dollars in cost a year, according to the Lasker Foundation.
The risk of developing Alzheimer's doubles every five years after age 65 and almost 40 percent of people over age 85 have the disease (J.M. Lyness et al., Ann.
Inter.
Med., 144: 496-504, 2006; and Lephart et al., Curr Neurovas. Res., 1: 455-464, 2004). Minor depression is more frequent than major depression, with almost 50% percent of older community residents (60 years or older) displaying symp-toms that are associated with minor depression (J.M. Lyness et al., Ann.
Inter.
Med., 144: 496-504, 2006).

[00151] In one embodiment, the invention includes the use of equol, includ-ing enantiomeric equol to treat and prevent diseases and conditions related to brain function and mental health, including brain disorders, such as Parkinson's dis-eases, dementia of the Alzheimer type, as well as other reduced or impaired cogni-tive functions associated with advancing age and with short- and long-term mem-ory loss. Brain mechanisms are more complex, and attempting to define what molecules and factors regulate, influence, etc., mood, depression, anxiety and so on, can be difficult. However, there are some data to support the concept that es-trogens or estrogen-like molecules like isoflavones can assist cognitive function in conditions such as Alzheimer's disease, and may help to prevent the onset of such disorders, especially in postmenopausal women.
[00152] It is well established that neuropsychiatric disorders represent the second largest cause of morbidity and premature mortality worldwide. The World Health Organization estimates, collectively, that neuropsychiatric disorders com-prise 13 % of all reported diseases. These disorders include depression, anxiety, schizophrenia, bipolar disorder, obsessive-compulsive disorder, panic disorder, bulimia, alcohol and substance abuse, overeating and obesity, and attention-deficit hyperactivity disorder (World Health Organization. The world health report - health systems: improving performance. Geneva: WHO 2000). Approximately one out of five Americans will experience an episode of a psychiatric illness such as depression or anxiety in any given year (Mental Health Report of the U.S.
Sur-geon General, 1999). Notably, the most common co-morbid psychiatric diagnoses with alcoholism are other substance abuse-related disorders such as, depression and anxiety disorders (R. Hitzemann, Alcohol Res. & Health 24: 149-158, 2000).
[00153] Depression is an effective disorder, or a disorder of mood. Symp-toms include feelings of sadness, worthlessness, despair and loss of interest in life's activities or all pleasures. Most individuals suffering from depression also experience mental slowing, confusion, loss of energy and an inability to make de-cisions or concentrate. The symptoms can range from mild or minor to severe and are often associated with anxiety and/or agitation (Diagnostic Criteria form DSM-IV, American Psychiatric Association, Washington, D.C., 1994).
[00154] Depression has been referred to as the "common cold" of mental illness. It is a prevalent disorder with approximately 18 million adults in the U.S.
population afflicted on an annual basis (National Institutes of Healtlz, Invisible Disease: Depression. Bethesda, M.D., NIH publication 01-4591, 2001). It is es-timated to be the second leading cause of disability, surpassed only by heart dis-ease (C. J. Murray & A.D. Lopez (eds.), in The Global Buf-den of Disease, Har-vard Univ. Press, Boston, 1996). Worldwide, its prevalence is 21 % in women and 13 % in men (C.B. Nemeroff and M.J. Owens, Nature Neurosci., 5: 1068-1070, 2002).

[00155] Although research continues as to the underlying causes of depres-sion, it is generally believed that the disorder stems from decreases in synaptic concentrations of neurotransmitters or associated mechanisms of neurotransmitter action. Antidepressants are the current mode of therapy. Unfortunately, most an-tidepressants typically require several weeks of administration before clinical ef-fects are apparent, despite the fact that their biochemical actions occur almost im-mediately. Finally, there can be concerns about side effects, contra-indications, and safety of antidepressants.

[00156] Anxiety disorders are often characterized by excessive worry, fear and apprehension, occurring frequently and causing a significant impairment of functioning in a daily routine. Symptoms can include restlessness, fatigue, diffi-culty concentrating, irritability, muscle tension and sleep disturbances. The life-time prevalence of anxiety disorders in the general population is approximately 5 to 6% (D.J. Nutt et al., Int. J. Neuropsychopharm., 5: 315-325, 2002). Women are affected more frequently than men, with a prevalence of 10% in women over the age of 35, anxiety rates tend to increase in midlife and in older adults (H.W.
Wit-tchen, Depress. Anxiety, 16: 162-171, 2002), that are similar to that seen for de-pression.

[00157] Current methods of treatment of anxiety includes pharmacological treatment witli compounds, including anxiolytics, such as benzodiazepines (e.g., diazepam, valium). Benzodiazepines possess anxiolytic, sedative-hypnotic, anti-convulsant and muscle relaxant properties. However, if the circulating plasma levels exceed the anxiolytic range, benzodiazepines may cause impairments of mental and motor function, confusion and amnesia. Benzodiazepines may also be subject to dose escalation and abuse by some patients. Withdrawal from long-term use can precipitate anxiety, insomnia, restlessness and agitation (R.M.
Julien, Prifner ofDrugAction, 7th ed., W.H. Freeman Co., New York, 1995; and M.E.

Likey and B. Gordon, Medicine and Mental Illness, W.H. Freeman Co., Boston, 1991).
[00158] In reference to mood, anxiety, depression, and other mental health conditions, there are two basic viewpoints. One line of research supports the view that estrogens (especially in women) regulate anxiety and help to decrease anxiety levels. Both estradiol and progesterone alter anxiety-related behaviors as well as the testicular androgen, testosterone (Imhof J. T. et al., Behav Brain Res, 56:177-180 (1993)). The anti-androgenic activity of equol acts in the brain by enhancing neurotransmission and restoring synaptic density. Without being bound by any particular theory, we believe that R- and/or S-equol are active in the brain at the same site(s) as estrogen, exerting an estrogenic response.
[00159] The second line of research is more complex and supports the view that 5a-reduced steroids, especially progesterone, have the ability to bind GABAA
receptors in the brain and cause sedation. GABAA is the major inhibitory neuro-transmitter in the brain and its receptors are abundant in brain areas that control mood/emotion. By causing sedation, individuals express less anxiety. For exam-ple, most women during pregnancy, report that they feel OK but they are usually tired or sleepy. This is due to progesterone being converted by the 5a-reductase enzyme in the body, but especially in the brain to 5a-dihydroprogesterone (5a-DHP) and further metabolism of this molecule results in the most potent `neuros-teroid' that can bind the GABAA receptor and enhance the action of GABAA. Ad-ditional supporting evidence that this 5a-dihydroprogesterone molecule (and its metabolite) can decrease brain activity is seen in epileptic women (who experience epilepsy and hence seizures) where these individuals almost never experience sei-zures during pregnancy due to the high circulating levels of progesterone. It should be noted that 5a-reduced androgens like 5a-DHT also have a similar effect on GABAA receptors to cause sedation (in men) but at much lower levels com-pared to 5a-DHP.

[00160] Putting these two views together, estrogen on the one hand de-creases anxiety and hence increases activity. Conversely, blocking the action of 5a-DHP also increases activity and thus in behavioral tests is interpreted as de-creasing anxiety. For example, when the conversion of progesterone to 5a-DHP
in pregnant rats has been blocked, this results in a significant increase in their lo-comotor activity levels.
[00161] Taking a similar perspective on this in reference to equol, equol has the ability to bind 5a-DHP (mainly seen in women) and 5a-DHT (mainly seen in men). This would decrease the potent `neurosteroid' effects at the GABAA recep-tor and decrease sedation and thus increase activity or decrease anxiety.
Moreover, the ability of S-equol to bind the estrogen receptor(s) beta would also increase ac-tivity. Finally, our studies using young- or mid-aged adult rats, in males or fe-males have shown that dietary phytoestrogen consumption (Lund T. D. et al., Bt=ain. Res, 913:180-184 (2001); Lephart, E. D. et al., Neurotoxicology Teratology, 24:1-12 (2002)), or injections with equol significantly decrease anxiety levels as expressed in the elevated plus maze test.
[00162] The elevated plus maze is a behavioral test used to quantify anxiety-related behavior and identify anxiolytic drugs (Pellow S. et al., JNeurosci Meth-ods, 14:149-147 (1985); Current Protocols In Neuroscience (1997) 8.3.1-8.3.15, John Wiley & Sons. NY, N.Y.). The test relies on the inherent conflict between exploration of a novel environment and avoidance of its aversive features. Nor-mally, animals spend little time and make few entries into the open arms of the maze compared to the closed arms of the maze (Imhof J. T. et al., , Behav Brain Res, 56:177-180 (1993). However, animals treated with anxiolytics, such as ben-zodiazepines (valium), spend more time in the open arms and the number of en-tries into the open arms reflects a decrease in anxiety-related behaviors (Pellow S.
et al., JNeurosci Methods, 14:149-147 (1985); Current Protocols In Neuroscience (1997) 8.3.1-8.3.15, John Wiley & Sons. NY, N.Y.; Chopin P. et al., Psycho-pharm, 110:409-414 (1993).
[00163] The invention also includes the use of equol (R- and S-enantiomeric mixtures, etc.) to treat or prevent peri- or postmenopausal symptoms. These symptoms include mood alterations, depression, anxiety, fatigue and emotional and mental health, weight gain and obesity, and polycystic ovarian disease among others [00164] In another embodiment, the invention is a method of use of equol to prevent or treat neuropsychiatric diseases, including depression, anxiety, and oth-ers mentioned above.
[00165] The invention includes the use of enantiomeric equol to treat and prevent diseases and conditions related to body weight and body fat formation.
Weigh gain and obesity represent a huge health concern in the U.S. and developed countries throughout the world. For example, in the U.S., about 55 % of adults are overweight by international standards, about 23 % of American adults are consid-ered obese; and one in five American kids are considered overweight. Obesity in the U.S. cost the United State Government 12 % of the national health care budget in the late 1990s or $118 billion (World Watch Institute, March, 4, 2000).
[00166] Phytoestogens including equol, have the ability to decrease the for-mation of white adipose (fat) tissue and increase white adipose tissue breakdown, thus decreasing body weight. Also, the estrogen-like nature of phytoestrogen molecules decreases LDL (so-called "bad" cholesterol), blood pressure, and pre-vents insulin resistance (or in other words, provides beneficial effects to the dia-betic condition). Since equol is a more potent isoflavone inolecule compared to the other phytoestrogeiis, it presumably provides the health benefits and protects against the conditions outlined above.
[00167] Since equol binds 5a-DHT, equol can also block the actions of 5a-DHT that promote body weight gain. Thus, equol, and particularly S-equol, com-bined anti-androgenic but at the same time an estrogenic hormone action from the same molecule (equol) would further improve the health benefits of body weight loss (and weight management), decrease LDL cholesterol, decrease blood pres-sure, and help prevent the devastating effects of diabetes.
[00168] The invention includes the use of equol to prevent or treat increase in body weight and obesity. The term "increase in body weight" refers to any in-crease in body weight above an optimal healthy level that would be dependent upon age, sex and statute of the subject. A person is considered to be obese if they are more than about 20 % over their ideal weight. That ideal weight must take into account the person's height, age, sex, and build. Overweight or obesity may also be defined using the body mass index, for instance. For adults, overweight and obesity ranges are determined by using weight and height to calculate a num-ber called the "body mass index" (BMI). BMI is used because, for most people, it correlates with their amount of body fat. An adult who has a BMI between ap-proximately 25 and 29.9, is considered overweight. An adult who has a BMI of around 30 or higher, is considered obese. Being overweight or obese increases the risk of many diseases and health conditions, including the following:
hypertension, dyslipidemia (for example, high total cholesterol or high levels of triglycerides), type 2 diabetes, coronary heart disease, stroke, gallbladder disease, osteoarthritis, sleep apnea and respiratory problems and some cancers (endometrial, breast, and colon) (U.S. Department of Health and Human Services, Center for Disease Con-trol (CDC) and Prevention, 2006).
[00169] The invention includes the use of equol, as enantiomeric equol or mixture thereof, to treat and prevent lipid disorders such as high cholesterol (hy-percholesterolemia), lipidemia, lipemia and dyslipidemia (disturbances in lipids).
A study has shown that plasma total cholesterol concentrations decreased 7.2%
(p=0.04) in equol producers compared with baseline levels and 3.0% (p NS) in non-equol producers. The failure of soy protein to have significant cholesterol-lowering effects in adults with normal blood cholesterol levels, is, with few excep-tions, probably because of heterogeneity in the study populations with regard to the metabolism of soy isoflavones and the failure to recognize the relevance of equol formation (and specifically, non-formation in non-equol producers).
These data suggest that enantiomeric equol influences lipids in a favorable manner, and that the effect is mediated by androgens. The composition comprising equol is administered in an amount sufficient to reduce the level of lipids in the blood stream.
[00170] The invention further includes the use of R- and/or S-equol to im-prove diminished blood vessel quality, by increasing reactivity or flexibility in re-sponse to acute changes in blood pressure, improving blood flow, and reducing blood pressure. The invention also includes the use of R- and/or S-equol to treat and prevent cancer, including benign prostate cancer, prostate cancer, and skin cancer.
[00171] Another embodiment of the present invention is the use of equol to treat enlarged prostate or epididymis. Equol may also be used to prevent enlarged prostate or epididymis in individuals believed to be at risk for development of these pathologies, without alterations in testes, pituitary or body weights.
The equol may be administered by any route that allows absorption of equol to the blood stream.

DHT-Androgen Receptor [00172] Other embodiments of the present invention include the use of equol as a diagnostic agent in androgen-related disorders as well as disorders arising from disturbances in estrogenic/androgenic balance. In these embodiments, equol is administered to an individual to bind 5a-DHT and thereby prevent 5a-DHT
binding to androgen receptors. The changes in estrogenic balance are then meas-ured or the change in androgen-binding is assessed to diagnose or further elucidate androgen-related anomalies.

Binding to 5a-DHT
[00173] Equol can be administered to bind 5a-DHT prior to or along with other therapeutic moieties in order to assess the binding capacity of 5a-DHT
with respect to the therapeutic moiety in question. Also, androgen-binding moieties can be administered following administration of equol to assess the efficacy of the androgen-binding moiety to restore androgen activity and balance estrogenic ac-tivity in the absence of 5a-DHT binding. Further, equol can be administered in the presence of 5a-DHT-binding moieties in order to displace these naturally-occurring or xenobiotic 5a-DHT-binding moieties from 5a-DHT.

Administration of Equol [00174] In each of the embodiments of the present invention described herein, if the administration of equol is to be oral, the equol may be administered by supplying an oral dosage form of equol to either an "equol-producing"
mammal or a "non-equol producing" mammal, or an oral dosage of daidzein, daidzin, isoflavone mixtures containing daidzein, or soy protein preparations to an "equol-producing" mammal, wherein the administration of the oral dosage form results in effective absorption of equol to the blood stream. Administration of equol may be made by routes other than oral, if desired. For example, it is contemplated that rectal or urethral administration may be used to administer equol for the treatment of enlarged prostate or to prevent prostate enlargement. Additionally, it is con-templated that the active ligand binding site of the equol molecule may be isolated and synthesized for administration, which may provide 5a-DHT binding without the full equol molecule. The dose of the equol molecule or fragment thereof hav-ing 5a-DHT-binding abilities is dependent upon the route of admiration and the condition to be treated. Based on our in vivo studies it is apparent that relatively low doses of equol antagonize much higher doses of 5a-DHT, and this may be ex-plained by the marked differences in the binding of equol to serum protein com-pared with 5a-DHT. The latter circulates mostly bound to proteins, while equol is 50% free. Generally, a dose sufficient to produce a concentration of equol or ac-tive fragments thereof in the bloodstream of the recipient of at least about 0.2 mg equol per kg weight of the recipient and preferably at least about 0.5 mg/kg.
The dose may be increased dramatically without incurring significant dose-limiting side effects to greater than about 10 mg/kg. Oral administration can be effected in microencapsulated forms that can provide delayed or sustained release of the me-dicament.

[00175] Equol can be administered topically, transdermally, and subder-mally in a variety of forms, including lotions, ointments, foams (including shaving creams), nasal sprays, skin patches (such as described in U.S. Pat. No.
5,613,958, incorporated herein by reference in its entirety), electromechanical devices, includ-ing micropumps systems (such as described in U.S. Pat. No. 5,693,018 and U.S.
Pat No. 5,848,991, incorporated herein by reference in their entirety), and subder-mal implants (such as described in U.S. Pat. No. 5,468,501, incorporated herein by reference in its entirety).

Experiments (a) Determination of Equol Enantiomer in `Equol-Producing' Adults [00176] The urine samples from adults consuming soy foods previously identified as being `equol-producers' were analyzed. Equol was isolated from urine (25 mL) by passage of the sample through a solid-phase Bond Elut C 18 car-tridge. After washing the cartridge with water, the isoflavones were recovered by elution with methanol (5 mL) and the methanolic phase was taken to dryness un-der a stream of nitrogen. The sample was subjected to enzymatic hydrolysis with Helix pomatia and then re-extracted on a Bond Elut C18 cartridge. The methano-lic extract was taken to dryness under nitrogen gas and redissolved in HPLC mo-bile phase (100 L). Equol enantioiners were identified by HPLC using a Chiral-cel OJ chiral phase column as described herein above. The detection of equol was achieved by selected ion monitoring electrospray ionization mass spectrometry (ESI-MS). Mass chromatograms of a pure standard of S-equol, and of urine from an adult consuming soy food are shown in FIG. 3. Similar studies have demon-strated that soy-derived isoflavones are converted to equol in rats, as well, thus validating rodent models of isoflavone metabolism.
[00177] The retention index and mass chromatogram establish that it is ex-clusively the S-enantiomer of equol that is excreted in human urine as no detect-able R-enantiomer of equol could be found. Analysis of the plasma from the same `equol-producer' also revealed only the S-enantiomer of equol.

(b) Chemical Synthesis of Racemic Equol [00178] Daidzein (200 mg, 0.8 mmol) is dissolved in a mixture of glacial acetic acid (20 mL) and isopropanol (20 mL), and is reduced with 10% Pd on charcoal (150 mg) at 55 p.s.i.g. (3.7 atm gauge). At the end of the reaction (2 hours, TLC:isopropanol/n-hexane'/4) the catalyst is filtered off, and the filtrate is evaporated. The crude residue is purified by chromatography on a silica gel col-umn using as eluent a mixture of isopropanol and n-hexane (1:4 v/v), to give ( )equol as a pure product (160 mg, yield: 82%) crystallized from n-hexane.
The product, colorless crystals, is not hygroscopic, is stable in air, and does not de-compose during the final filtration procedure. The product of this chemical syn-thesis was in all respects identical with an authentic sample of ( )equol (racemic equol). FIG. 4 shows the GC-MS analysis of the trimethylsilyl ether derivative of synthesized product as a single pure peak and a mass spectrum that is consistent with the published electron ionization spectrum of the trimethylsilyl (TMS) ether derivative of authentic equol. The molecular ion as expected is at m/z 470 and the base peak at m/z 234. The purified equol product had a purity of greater than 99%, as confirmed by HPLC and mass spectrometry.

(c) Elution Order of S- and R-enantiomer by Optical Dichroism [00179] A racemic mix of S-equol and R-equol were separated by chiral chromatography on a Chiralcel OJ Column using a flow-rate of 1.0 mL/min and with a gradient elution consisting of an initial mobile phase of 10% ethanol in hexane and increasing to 90% ethanol in hexane over a time period of 15 minutes according to the program shown in Table 1:
[00180] TABLE 1:

Chiral Separation Gradient Eluent of Hexane and Ethanol Time (min.) % Hexane % Ethanol 1.0 90 10 15.0 10 90 16.0 90 10 17.0 90 10 [00181] FIG. 5 shows the mass chromatogram of the ions recording (m/z 241) for a racemic mixture of S- and R-equol.
[00182] The first eluting material, designated as Enantiomer- 1, and the sec-ond eluting material, designated as Enantiomer-2, were collected separately.
Each enantiomer was weighed and the weighed samples dissolved in 1 mL of spectro-scopic grade ethanol. Measurement of the optical rotation of each enantiomer was carried out at 200 C. using the light of wavelength in the line D of sodium.

[00183] Enantiomer-1 material (1.6 mg exact weight) had first and second measurements of -0.023 and -0.022, resulting in an optical rotation of -14[-0.0225 X 1000/1.6], wllich corresponds with the S-enantiomer of equol. Enanti-omer-2 material (1.7 mg exact weight) had first and second measurements of +0.023 and +0.023, resulting in an optical rotation of +13.5 [+0.023 x 1000/1.7], which corresponds with the R-enantiomer of equol.

d) Deter=mination of Receptor Binding Capacity of S- and R-Enantiomers [00184] In vitro binding studies were performed to examine the relative af-finities of S- and R-enantiomeric equol witli the estrogen receptors ERa and ER(3.
[00185] Synthesis of Hormone Receptor Proteins: Full length rat ERa ex-pression vector (pcDNA-ERa; RH Price UCSF) and ER(3 expression vector (pcDNA-ER(3; T A Brown, Pfizer, Groton, Colo.) were used to synthesize hor-mone receptors in vitro using the TnT-coupled reticulocyte lysate system (Promega, Madison, Wis.) with T7-RNA polymerase, during a 90 min reaction at 30 C. Translation reaction mixtures were stored at -80 C. until further use.
[00186] Saturation isotherms: In order to calculate and establish the binding affinity of the S-equol and R-equol enantiomers for ERa and ER(3, 100 L
aliquots of reticulocyte lysate supernatant were incubated at optimal time and temperature;
90 min at room temperature (ER(3) and 18 hrs at 4 C. (ERa), with increasing (0.01-100 nm) concentrations of [3H] 17(3-estradiol (E2). These times were de-termined empirically and represent optimal binding of receptor with estrogen.
Nonspecific binding was assessed using a 300-fold excess of the ER agonist dieth-ylstilbestrol, in parallel tubes. Following incubation, bound and unbound [3H]E2 were separated by passing the incubation reaction through a 1 mL lipophilic Sephadex LH-20 (Sigma-Aldrich Co., Saint Louis, Mo.) column Columns were constructed by packing a disposable pipette tip (1 mL; Labcraft, Curtin Matheson Scientific, Inc, Houston, Tex.) with TEGMD (10 mm Tris-Cl, 1.5 mm EDTA, 10% glycerol, 25 mm molybdate, and 1 mm dithiothreitol, pH 7.4)-saturated Sephadex according to previously published protocols (Wanda et al., 1986;
O'Keefe and Handa, 1990). For chromatography, the columns were re-equilibrated with TEGMD (100 L), and the incubation reactions were added in-dividually to each column and allowed to incubate on the column for an additional 30 min. Following this incubation, 600 L of TEGMD were added to each col-umn, flow-through was collected, 4 mL scintillation fluid was added, and samples were counted (5 min each) in an 2900 TR Packard scintillation counter (Packard Bioscience, Meriden, Conn.).
[00187] ' Competition binding studies were used to assess the estrogenic properties of equol's S-equol and R-equol enantiomers. Based on the ability of S
and R to compete with [3H] E2 for ER binding, the affinities for in vitro translated ER were shown to be very different for the two enantiomers. The S-equol enanti-omer showed greatest affinity for ER(3 [Kd (nm)=0.73 0.2], while its affinity for ERa was relatively low by comparison [Kd(nm)=6.41 1.01. The R-equol enanti-omer possessed a much lower affinity for both ER(3 [Kd (nm)=15.4 1.31 and ERa [Kd (nm)=27.38 3.8]. For reference 17(3-estradiol binds ERa with a Kd (nm)=0.13 and RE(3 with a Kd (rum)=0.15 in this system.
[00188] The study shows that only the S-equol enantiomer binds ER with sufficient affinity to have potential relevance to circulating equol levels reported in humans. Compared with 17p-estradiol the relative binding affinities of the S-equol and R-equol enantiomers for ERa were 49-fold and 211-fold less, respec-tively. However, the S-equol enantiomer seems to be largely ER(3-selective with a relatively high affinity for ER(3, while the R-equol enantiomer binds with ap-proximately 100-fold less affinity. The separate and associated determination that exclusively S-equol is found in human plasma and urine is significant in view of the specificity in binding of the two enantiomers.

e) Bioavailability of R-Equol [00189] 20 mg of pure R-equol was administered orally to a healthy adult after an overnight fast. Blood samples were collected at timed intervals over the next 24 hours and the plasma concentration of equol was determined by isotope dilution gas chromatography-mass spectrometry with selected ion monitoring.
Rapid appearance of equol is observed in the plasma with peak concentrations ob-served after 8 hours. The terminal elimination half-life of R-equol was approxi-mately 8 hours. Electrospray ionization mass spectrometry confirmed that the equol present in plasma was the R-equol enantiomer (data not shown but available on request), thereby establishing that it is stable and does not undergo any racemi-zation or further biotransformation in the intestine. FIG. 2A shows an appear-ance/disappearance plot of R-equol. FIG. 2B. shows an appearance/disappearance plot of S-equol and R-Equol in plasma (mean levels) after oral administration of S-equol or R-equol in healthy human adults (from: K.D. Setchell et al., Am J.
Clin.
Nutr., 81: 1072-1091, 2005).
[00190] These results establish that R-equol if administered as a pharma-cologic or nutraceutical preparation is extremely bioavailable.

EXAMPLES
[00191] Where appropriate, data were analyzed by analysis of variance sta-tisti6s (ANOVA) followed by Newman-Keuls post hoc tests. Significance was set at p<0.05. Curve fitting, scientific graphing, and analysis were completed using GraphPad Software (GraphPad Prism 3.0, San Diego, Calif.).

[00192] This example demonstrates the in vivo effects of equol on prostate size and hormone secretion. Male Sprague-Dawley rats (400-500 grams) are ob-tained from Charles Rivers Laboratories (Wilmington, Mass., USA). Rats are caged in pairs and maintained on a 12-hour dark 12-hour light schedule (lights on at 0700 h) with ad libitum access to food and water.
[00193] One week following arrival, animals are given subcutaneous (sc) injections (1/day for 4 days) of either dimethylsulfoxide (DMSO) (vehicle control) or racemic equol (0.25 mg/kg). Eighteen hours after the final injection, animals are killed via decapitation and trunk blood and prostate are collected for analysis.
[00194] A significant reduction in prostate weight is observed in intact males injected subcutaneously with equol in comparison to intact control males.
Additionally, luteinizing hormone (LH) in these same intact males is significantly increased in equol compared to control treated males. These finding are shown in FIGS. 6A and 6B, respectively. These effects are observed with relatively low levels of equol compared to 5a-DHT and this can be explained by the marked dif-ferences in the protein binding of equol, which circulates about 50% free, and 5a-DHT which is mostly bound to serum protein.

[00195] In addition to equol's effects on prostate racemic equol blocks the effects of DHT in other tissues, and decreases body weight. One week following arrival, intact'males are given subcutaneous injections of either DMSO
(control) or equol (0.5 mg/kg) once/day for 7 days. Following treatments animals are weighed and then killed via decapitation and tissue collection (prostate, testes, epididymis, and pituitary).
[00196] A significant weight decrease in the 5a-DHT-sensitive epididymis is observed in racemic equol-treated males compared to controls. However, racemic equol did not affect testes weight or pituitary weight. Body weight during this relatively brief treatment period does not differ significantly between racemic equol and control treatments. These results are presented in Table 2.
[00197] TABLE 2 Tissue Weights from Intact Males Given /Subcutaneously Injections of Either DMSO (Control) or Racemic Equol Prostate (g) Epididymis (g) Testes (g) Pituitary (g) Body (g) DMSO (Con- 0.58 (:L0.05) 1.91 ( 0.10) 3.52 (:L0.10) 0.011 (:L0.003) 428.23 (14.04) trol) Racemic Equol 0.38 (10.06)* 1.59 (_L0.06)* 3.48 (:L0.08) 0.014 (10.002) 418.0 (+-7.52) (0.25 mg/kg) [001981 Adult male Sprague-Dawley rats are randomly assigned to three groups and receive daily injections of either DMSO, a racemic mixture of equol at 0.250 mg/kg/day), R-equol at 0.250 mg/kg/day, or S-equol at 0.250 mg/kg/day.

The total volume of each injection is 0.3 cc, administered sc at the nape of the rat's neck. After seven consecutive days of treatment, the rats are killed and the body weight gain during the injection period is determined. Rats injected with R-equol have a significant decrease in body weight gain compared to control rats, as shown in Table 3.
[00199] TABLE 3 Body Weight Gained in Male Sprague-Dawley Rats Treated with Equol Treatment Group Prostate (g) Epididymis (g) Body Wt Gain (g) Control 0.38 ( 0.01) 0.96 (10.03) 59.4 (:L3.4) Racemic Equol (0.25 0.38 ( 0.02) 0.89 (:L.03) 56.1 ( -3.7) mg/kg) R-equol (0.25 mg/kg) 0.31 (-+0.02)* 0.85 (.1:0.04)* 45.6 ( 5.5)*
S-equol (0.25 mg/kg) 0.35 ( 0.03) 0.86 ( 0.05) 56.1 ( 3.1) *Significant reduction compared to control [00200] Testis and pituitary gland weights are not significantly altered by the treatments (data not shown). The slight decreases in body weight (around 10%) from the equol experiments are very similar to those seen between animals fed a Phyto-Free diet (a diet containing very low levels of phytoestrogens) vs. a Phyto-600 diet (a phytoestrogen-rich diet containing 600 ppm of isoflavones).
The significant reduction in white adipose tissue deposition with the racemic equol in-jections (around 36%) is also comparable with that seen with the data sets derived from the dietary treatment studies. These findings suggest that R-equol can regu-late body weight and significantly decrease white adipose deposition.

[00201] This Example demonstrates equol binding to 5a-DHT. In initial binding competition studies conducted to determine and establish equol's binding affinity for AR, we repeatedly observed that the apparent binding of [3H]DHT
was greater in the presence of equol than in its absence. Slight modifications in the protocol where AR was removed from the incubation tube (leaving only [3H]DHT and equol) resulted in the elution of [3H]DHT into the eluate containing [3H]DHT reaction complex [00202] To further investigate this phenomenon, a 30 cm long Sephadex LH-20 columns are used in order to identify elution peaks establishing the binding of [3H]DHT to equol. As shown in FIG. 7, a peak of [3H]DHT is apparent in the elution fractions between 5 and 9 mL when the [3H]DHT+equol column incubate is applied. This peak is not present when [3H]DHT alone is applied to the col-umn. Furthermore, when 5a-DHT or 5a-DHT+equol are incubated with prostate supernatant and then passed through the 30 cm column (FIG. 8A) two distinct binding peaks are identifiable. The first peak of [3H]DHT represents that bound to the AR in prostate. This is found in the elution fractions between 4 and 5 ml.
In addition there is a later peak (between 5 and 9 ml), consistent with the binding of [3H]DHT to equol. However, when [3H]DHT is allowed to incubate with the prostate supernatant for 36 hours (until equilibrium) prior to the introduction of equol there is no apparent binding of [3H]DHT (FIG. 8B). Both [3H]DHT and [3H]DHT+equol (equol added 36 hours later) show a single peak in the elution between 4 and 5 ml, suggesting that equol does not compete with 5a-DHT for the AR nor does it bind [3H]DHT that is already bound to the receptor.
Furthermore, it should be noted that the binding of equol to 5a-DHT appears to be specific, since similar competition and binding studies have been conducted using [3H]E2, [3H]T, [3H]DHEA, [3H]CORT and [3H]progesterone without any occurrences of binding to equol (data not shown). Saturation analysis of equol binding to [3H]DHT shows an apparent Kd calculated at 1.32 0.4 nM (FIG. 9).

[00203] This example demonstrates that, in addition to modulating the effect of 5a-DHT on prostate size, equol binds to 5a-DHT in vivo and blocks the nega-tive effects on LH secretion. GDX males treated with a long-lasting analog of 5a-DHT, DHT propionate (DHTP), show a significant increase in prostate weight compared to vehicle-treated GDX control rats. Concomitant treatment with equol (DHTP+equol) blocks the effects of DHTP, while equol has no effect alone on prostate size as shown in FIG. 10A. Equol also blocks 5a-DHT's negative feed-back effects on LH. In GDX males LH is significantly decreased by DHTP treat-ment compared to treatment with DMSO. Treatment with equol in combination with 5a-DHT blocks the negative feedback effects of DHTP on LH secretion.
Equol alone has no effect on LH levels, shown in FIG. IOB.

[00204] This example demonstrates the effects of racemic equol on andro-gen-sensitive tissues. One week following arrival, animals are gonadectomized (GDX) under isoflurane anesthesia and allowed to recover for 7 days. Following recovery, animals are assigned to the following groups 1) DMSO, 2) DHTP
(2mg/1 kg), 3) racemic equol (0.25 mg/kg), or 4) both DHTP and racemic equol.
Injections are given subcutaneously daily for 4 days. Animals are killed via de-capitation and trunk blood and tissues are collected for analysis. Plasma 5a-DHT
is measured, shown in FIG. 11.
[00205] As expected there were significant elevations of plasma 5a-DHT in animals treated with DHTP (GDX+DHTP, GDX+equol+DHTP groups). Plasma 5a-DHT was further elevated, although, not significantly, by co-treatment with equol. Tissues, including prostate, testes and epididymis, are removed from the animal, dissected free of fat and connective tissues, weighed, fixed by immersion in 4% paraformaldehyde, and then sectioned at 15 m on a cryostat. Tissue sec-tions are mounted on charged slides (Superfrost Plus, Fisher Scientific, Pittsburgh, PA) prewarmed to 23 C, and stained with hematoxylin and eosin (H&E), dehy-drated in ascending alcohol and cleared with xylene. Histological sections are shown in FIGS. 12 and 13. H&E stained prostates reflect a change due to both GDX and treatments. The prostate glands of control, equol, and DHTP plus equol treated groups show similar histology (FIG. 12 A, B, D). In these animals pros-tates are characterized by very small atrophic glands with little volume in the gland lumen. In DHTP-treated animals (FIG. 12C), the glands show signs of cell proliferation. Lumen size is increased compared to GDX animals; the epithelium is of a tall columnar type (FIG. 12C). In comparison to intact control animals (FIG. l2E) the prostate of equol treated males show involution and consist of more closely spaced, atrophic glands (FIG. 12F). In comparison to control males, the epididymal histology of equol-treated intact males shows overall smaller ducts, as evidenced by shrunken lumen (FIG. 13).

[00206] Long-Evans male rats are raised (life long from conception to time of sample collection) on either a phytoestrogen-rich diet containing 600 micro-grams of isoflavones per gram of diet or 600 ppm of isoflavones (referred to here-after as the "Phyto-600" diet) or a diet containing very low levels of isoflavones (referred to hereafter as the `Phyto-Free' diet; containing approximately 10 ppm of isoflavones).
[00207] As shown in FIG. 14 male Long-Evans rats fed the Phyto-600 diet display significantly lower body weights at 33, 55 or 75 days of age compared to animals fed the Phyto-Free diet Adipose tissue (dissected from just below the kid-neys to just above the testes in the abdominopelvic cavity) is measured in the 55 or 75 day-old males. At both ages, white adipose tissue mass is significantly greater in the Phyto-Free-fed males compared to Phyto-600-fed animals, FIG. 15. It should be noted that the reductions in body weight of Phyto-600-fed males are modest, at approximately 10 to 15% percent, whereas, the reductions in white adi-pose tissue from the same animals are approximately 50-60% compared to Phyto-Free-fed males. This greater reduction in white adipose tissue compared to body weight in soy fed animals is also a general characteristic seen in humans consum-ing soy-based diets (D. B. Allison et al., Eur J Clin Nutr, 2003, 57:514-522).
This particular result is repeatedly seen throughout the various experiments present in these data sets, regardless of age, sex, rat strain or whether female rats have their ovaries removed (simulating the postmenopausal condition in humans).
[00208] When food and water intake is measured to determine whether these parameters might influence body and adipose tissue weights, Phyto-600-fed males display slight but significantly higher food (FIG. 16A) and water (FIG. 16B) in-takes compared to Phyto-Free-fed animals. Thus, the reductions in body and adi-pose tissue weights cannot be explained by alterations in food/water intake be-tween the diet treatments.
[00209] Since leptin is produced by adipose tissue (D. A. Sandoval et al., J
Diabetes Complications, 2003, 17:108-113.), serum leptin levels are measured, along with insulin levels, to determine the alterations in these metabolic hormones.
As shown in FIG. 17A, leptin levels at 33, 55 or 75 days of age are significantly decreased in Phyto600-fed males (which corresponds with the reductions seen in adipose tissue weights from these same animals) compared to Phyto-Free-fed males. Also, insulin levels are significantly decreased in Phyto-600-fed male vs.
Phyto-Free-fed males (FIG. 17B), consistent with the benefits of protecting against insulin resistance associated with type-2 diabetes (V. Jayagopal et al., Diabetes Care, 2002, 25:1709-1714.).
[00210] To demonstrate that circulating isoflavone levels are different in Phyto600- vs. Phyto-Free-fed male and female (75 day-old) rats, serum isoflavone levels are determined by GC/MS as previously performed by our laboratories (see methods in K. D. R. Setchell, Am J Clin Nutr 129:1333S-1346S, 1998; and K. D.
R. Setchell et al., JNutr 132:3577-3584, 2002.). In each case for the different classifications of isoflavones Phyto-600-fed males display significantly higher isoflavone levels compared to Phyto-Free-fed values, shown in Table 4. More im-portantly, equol levels in the Phyto-600-fed rats account for approximately 78% of the total phytoestrogen levels.
[00211] TABLE 4 Isoflavone Concentrations in Adult Male and Female Rats Genistein Daidzein Equol Total (ng/ml) Males:

Very low Isofla- 9.6-+0.3 10.8 0.6 23.2+0.4 43.5 1.0 vone Diet High Isoflavone 413 67 394 58 1,161 325 1,967 45 Diet Females:
Very Low 3.9 0.2 5.3 0.8 21.6 1.2 30.8 2.2 Isoflavone Diet High Isoflavone 99 9 117 7.4 931 21 1,147 5 Diet [00212] To determine if other metabolic hormones were altered by the diet treatments or by age, serum glucose and tliyroid (T3) levels are assayed.
Glucose levels are slightly (but not significantly) higher in the Phyto-600-fed males com-pared to Phyto-Free-fed values, independent of age or source of the blood samples [either arterial (ART) or venous (TRUNK)], shown in FIG. 18. However, when T3 levels are quantified, there is a significant increase in T3 serum levels in 80 or 110 day-old male Long-Evans rats fed the Phyto-600 diet compared to Phyto-Free-fed animals, shown in FIG. 19. This suggests that thyroid levels are en-hanced with soy consumption consistent with anecdotal evidence of individuals that decreased their thyroid medication or went off of thyroid treatment com-pletely with the consumption of soy based foods in their diets. This is also consis-tent with reports of a similar increase in T3 levels in humans following consump-tion of soy foods (Watanabe, S. et al., Biofactors 2000: 12(14):23341).

[00213] In this experiment female Long-Evans rats are raised (life long from conception to time of sample collection) on either Phyto600 or Phyto-Free diets.
As shown in FIG. 20, rats fed the Phyto-600 diet display significantly lower body weights at 80 days of age compared to animals fed the Phyto-Free diet, represent-ing about a 12% reduction in body weight in the Phyto-600-fed animals.
[00214] As seen in the male Long-Evans Phyto-600-fed rats previously, fe-males fed the Phyto-600 diet also displayed significant reductions in adipose tissue weight (by about 68%) compared to females fed the Phyto-Free diet, shown in FIG. 21.

[00215] Similar to results with male rats, serum glucose levels are slightly but not significantly higher in Phyto-600-fed female rats at 80 or 10 days of age compared to animals in the Phyto-Free diet treatment group, shown in FIG. 22.
However, T3 levels are significantly higher in females fed the Phyto-Phyto-600 diet compared to Phyto-Phyto-Free fed animals at 110 days of age, shown in FIG.
23. The T3 and glucose results in females are very similar to those obtained in male rats exposed to the same diet treatments, and thus, suggest similar health benefits for both genders. Samples collected at 100 days of age yield similar re-sults (i.e., significant reductions in body weight and adipose tissue weights with the consumption of the Phyto-600 diet vs. the Phyto-Free diet) in female Long-Evans rats (data not shown).

[00216] Adult female rats are placed on the Phyto-600 or Phyto-Free diet treatments from 50 to 215 days of age. Prior to 50 days of age the animals can be raised on a diet that contains approximately Phyto-200 ppm of isoflavones, or similar a diet such as those used by animal suppliers. At 215 days of age, Phyto-600-fed females weigh significantly less than Phyto-Free-fed females, represent-ing about a 7% reduction in body weight, shown in FIG. 24. White adipose tissue in Phyto-600-fed females at 215 days of age is significantly reduced by about 30%
compared to that of females fed the Phyto-Free diet, shown in FIG. 25. Corre-spondingly, serum leptin levels in the Phyto-600-fed females were significantly lower than those of Phyto-Free-fed, shown in FIG. 26. Insulin levels were reduced in the Phyto-600-fed vs. Phyto-Free-fed females to a similar degree seen previ-ously, but did not reach statistical significance, shown in FIG. 27.

[00217] This example demonstrates the effects of a Phyto-600 or Phyto-Free diet on adult ovariectomized (OVX) rats. The OVX rat is a well-established ani-mal model of postmenopausal human females. In addition, OVX permits the sub-cutaneous injections of estrogen and progesterone to stimulate behavioral estrus in rats, to determine the effects of a Phyto-600 or Phyto-Free diet. Adult ovariec-tomized rats are fed a phytoestrogen diet of approximately 200 ppm of isoflavones ("Phyto-200") until 50 days of age (all animals are ovariectomized at approxi-mately 40 days of age). The female rats are age and weight matched at 50 days of age and placed into one of two diet treatments: either the Phyto-600 (black bars) or Phyto-Free (white bars) until 94 days of age. Baseline body weights are taken at 50 days of age before the animals are placed on the diet treatments, again at days (8 days of diet treatment), at 92 days of age (before injection of estradiol), and at 94 days of age (before injection of progesterone, and 6 hours later at days of age (after chemical induction of behavioral estrus), shown in FIG. 28.
[00218] After consuming the diets for 8 days, the Phyto-600-fed rats display a slight but significant reduction in body weight (of about 7%) compared to Phyto-Free-fed. This reduction in body weight is maintained before and during the chemical induction of behavioral estrus by the estrogen and progesterone (steroid) injections.
[00219] White adipose tissue is measured at 94 days of age after the chemi-cal induction of behavioral estrus, Phyto-600-fed OVX rats have approximately 50% less white adipose tissue mass compared to Phyto-Free-fed OVX rats, shown in FIG. 29, consistent with findings in Examples 9 and 10.
[00220] Serum leptin levels in Phyto-600-fed OVX rats are decreased by approximately 30% compared to Phyto-Free-fed rats, shown in FIG. 30, reflecting the decreased white adipose tissue mass.

[00221] Male and female Long-Evans rats are purchased from a supplier at 50 days of age. All animals are raised (from conception to 50 days of age) on the Phyto-200 diet At 50 days old the male and female rats are randomly assigned to one of four diet treatment groups: 1) AIN-76 diet containing approximately <5 ppm isoflavones, 2) the Phyto-Free, 3) Phyto-200, or 4) Phyto-600 diet, described in previous examples. The AIN-76 diet contains extremely low concentrations of isoflavones, its formulation is quite different compared to the other three diets.

For example, the sucrose content is very high (almost approaching 50% of the to-tal diet formulation) and has a dense white consistency that the rats may not enjoy consuming as much as the regular plant-based ingredient diets (i.e., the Phyto-Free diet uses corn and wheat in its formulation which contains very low levels of isoflavones); the Phyto-200 or Phyto-600 diets use varying amount of soy meal in their formulations. The male rats are maintained on their assigned diets until days of age (equivalent to middle-age in humans). The female rats are maintained on the diets until 279 days of age (approaching middle-age in humans). Food and water intake is measured to determine the potential influence of these parameters on body weight changes. In each case these factors do not contribute to the reduc-tions in body weight with consumption of the isoflavone-containing diets (i.e., Phyto-200 and Phyto-600 diets; data not shown).
[00222] Males-[00223] At 112 days of age (on the diets for approximately 62 days), body weights are recorded, shown in FIG. 31. The males fed the Phyto-Free diet have the heaviest body weights and the Phyto-600-fed males have the lowest, while the males on the AS-76 and the Phyto-200 diets fall in between these two group val-ues. The Phyto-600-fed body weights are significantly lower, by approximately 14%, than the Phyto-Free-fed males.
[00224] Correspondingly, at 279 and 350 days of age the male rats have a similar profile to that observed at 112 days of age, shown in FIGS. 32 and 33, re-spectively. The males fed the Phyto-Free diet display the heaviest body weights and the Phyto-600-fed males display the lowest body weights, while the males on the AN-76 and Phyto-200 diets fall in between these two group values.
[00225] Males fed the AIN-76 or the Phyto-Phyto-Free fed males display the highest white adipose tissue weights, measured at 350 days of age. The Phyto-200-fed males show a 19% non-significant reduction in white adipose tissue weight compared to AIN-76 or Phyto-Free-fed rats. Male rats fed the Phyto-600 diet have significantly less adipose tissue mass, an approximate 40%
reduction, compared to AIN-76 or Phyto-Free-fed rats, shown in FIG. 34.

[00226] Both serum insulin and leptin levels are significantly reduced as a function of increasing concentrations of isoflavones in the diet treatments, shown in FIGS. 35 and 36, respectively. For example, males fed the Phyto-200 or Phyto-600 diets have significant reductions in insulin levels compared to AIN-76 fed males. Also, Phyto-600-fed males show an approximate 50% reduction in insulin levels compared to Phyto-Free-fed male Serum leptin profiles display a similar pattern to that of the insulin results, where Phyto-200- or Phyto-600-fed males have significant reductions in serum insulin levels compared to either AIN-76 or Phyto-Free-fed males. Insulin levels in the Phyto-600-fed males are 46% lower compared to the Phyto-200-fed males. However, the difference between these two diet groups do not reach significance (p<0.065).
[00227] Females-[00228] To determine the influence of the four diet treatments on body weight in female rats, the body weights are measured at 112 and 279 days of age.
At 112 days of age, females fed the Phyto-Free and the Phyto-200 diets have the heaviest body weights and the Phyto-600-fed females have the lowest, while the AIN-76 diet group fall in between the values of the other three groups, shown in FIG. 37. Body weights of the Phyto-600-fed groups are significantly lower, by approximately 10%, compared to the Phyto-Free- and the Phyto-200-fed females.
[002291 At 279 days of age, female rats have a similar profile to that of age-matched males for changes in body weight as influenced by the diet treatments, shown in FIG. 38. Females fed the Phyto-600 diet display the lowest body weights compared to AIN-76 or Phyto-Free-fed groups. This significant reduction in body weight in Phyto-600-fed females is approximately 15% between the diet treatment groups tested.

[00230] Noble rats were used to determine whether an inbred strain of rat has body and adipose tissue changes similar to those of out-bred strains of rats such as the Long-Evans animals when placed on isoflavone-rich diets. Due to in-breeding, Noble rats are more fragile animals. For example, pregnant dams do not always carry their litters to term and frequently have smaller litters.
Noble rats have been used for more than twenty years because they spontaneously generate tumors with aging, especially in hormonal-dependent organs of the reproductive tract. Thus, Noble rats have been extensively studied in the area of cancer re-search (e.g., R. L. Noble, "Prostate carcinoma of the Nb rat in relation to hor-mones," Int Rev Exp Pathol, 1982, 23:113-159).
[00231] Male and female Noble rats are fed either the Phyto-Free or Phyto-600 diets from conception until 145 days of age. Male Noble rats fed the Phyto-600 diet have significantly lower body weights at 145 days of age compared to age-matched males fed the Phyto-Free diet, shown in FIG. 39. As previously ob-served for Long-Evans rats, the significant reduction in body weight represents a modest but consistent decrease of approximately 8% compared to Phyto-Free-fed males. In addition, white adipose tissue mass is significantly decreased in Phyto-600-fed males compared to Phyto-Free-fed, shown in FIG. 40.
[00232] Female Noble rats fed the Phyto-600 diet have a 6% reduction in body weight compared to Phyto-Free-fed females, shown in FIG. 41. White adi-pose tissue mass is markedly decreased female Noble rats fed the Phyto-600 diet, shown in FIG. 42. The Phyto-600 diet group has a 61% reduction in adipose tis-sue compared to Phyto-Free- fed rats. The decrease in white adipose tissue is similar to that seen in Long-Evans rats.

[00233] Prior to initiation of a Phyto-Free diet period Male Long-Evans rats are fed a Phyto-200 diet, as described in previous examples. The rats are placed on a diet containing the Phyto-Free diet at approximately 52 days of age and ran-domly assigned to three groups. Baseline body weights after 14 days and 21 days on the Phyto-Free diet for all rats are similar, shown in FIGS. 43 and 44, respec-tively. Beginning at 73 days of age, rats receive daily subcutaneous 0. 1 cc injec-tions of vehicle (peanut oil), 1 milligram of a racemic mixture of equol in vehicle (0.83 mg/kg body weight/day), or 5 milligrams of a racemic mixture of equol in vehicle (4.2 mg/kg body weight/day) once every three days.

[00234] At 80 and 88 days of age, there are slight decreases in body weights and average body weight gains in both equol-injected groups compared to con-trols, however, these values are not significantly different from controls, shown in FIGS. 45 and 46, respectively.
[00235] By the time the animals are 95 and 101 days of age, body weights are only slightly decreased, ranging from 5 to 9% in equol-treated groups, shown in FIGS. 47 and 48. However, the average body weight gains in equol-injected animals at both 95 and 101 days are significantly reduced compared to control values. Though body weight differences are not significant, adipose tissue deposi-tion is strikingly lower in equol-treated groups. Adipose tissue mass in 101-day-old rats injected with equol is reduced by approximately 33% compared to con-trols, shown in FIG. 49.
[00236] To determine whether equol injections have an adverse effect on male reproductive organs, testis weights are quantified in these animals.
There are no significant alterations in testes weight with the equol injections, with testicular weight essentially the same among the injection treatment groups, shown in FIG.
50.

[00237] Fifty day-old Long-Evans males and females are caged individually and maintained on a 10-hour dark 14-hour light schedule (lights on 1400-0400).
Animals are randomly assigned to diet groups, and allowed ad libitum access to one of four diet treatments: 1) AIN-76, 2) Phyto-Free, 3) Phyto-200, or 4) Phyto-600 diet. The rats remain on the diets until mid-aged (at approximately 300 days of age in males and at approximately 330 days of age in females) when the ani-mals are tested in the elevated plus maze and anxiety-related behaviors were quan-tified. Thereafter, serum phytoestrogen levels are quantified by GC/MS
according to the method described by Coward L et al., JAgric Food Chenz, 41:1961-1967.
The behavioral patterns of anxiety are compared to the serum profiles of circulat-ing isoflavone levels in the diet treatment groups by sex.

[00238] In males, there is a dose-dependent expression of anxiety-related behaviors where animals fed the highest concentration of isoflavones display the lowest anxiety parameters. In contrast, animals fed the AIN-76 diet display the highest levels of anxiety, shown in FIG. 51. When the percent of time spent in the open arms is analyzed a similar pattern is seen to that of the nunlber of entries into the open arms. Notably, the Phyto-600 fed males display the highest percentage of time spent in the open arms, while the lowest percentage of time spent in the open arms is display by animals fed the AIN-76 diet, with Phyto-free and Phyto-200 values falling in between these maximal responses in a dose-dependent fash-ion, shown in FIG. 52.
[00239] Prior to testing in the elevated plus maze, females are monitored by vaginal smears for 12 consecutive days to verify that none are cycling to minimize effects of the estrous cycle. Female rats have a similar pattern of anxiety-related behaviors as those observed in the male rats. However, the influence of dietary isoflavones is not as robust as that seen in males. Although, the highest percent-age of the number of entries into (FIG. 53) or time spent on the open arms (FIG.
54) is seen in Phyto-600-fed females, with a stair-step pattern of decline until the lowest percentage of entries is seen in the AIN-76-fed females.
[00240] When behavioral testing is complete, the serum phytoestrogen lev-els are determined and compared to the patterns of anxiety-related behaviors.
In both males (FIG. 55) and females (FIG. 56), the circulating isoflavone levels cor-respond to the expression of anxiety-related behaviors, demonstrating an associa-tion between circulating isoflavone molecules and anxiety. These data demon-strate that the isoflavone content of a diet can have significant effects on anxiety.

[00241] Adult male Sprague-Dawley rats receive daily injections of either DMSO, racemic equol (0.250 mg/Kg/day), R-equol (0.250 mg/Kg/day), or S-equol (0.250 mg/Kg/day) in a total volume of 0.3 cc DMSO by subcutaneous in-jection. At the end of seven consecutive days of treatment the animals are tested in the elevated plus maze in order to quantify anxiety-related behaviors. As shown in Table 5 below, males injected with racemic equol or R-equol display a signifi-cant decrease in anxiety levels compared to control rats.
[00242] TABLE 5 Anxiety-Related Behaviors in the Elevated Plus Maze of Equol Injected Male Rats Injection Center Area Open Arm Time Open Arm Entries Treatment Groups (in seconds) (in seconds) (in seconds) DMSO 17.5+4.1 16.3+4.0 1.0+0.03 Equol (racemic) 37.1 + 6.3* 40.9 + 7.0* 2.3 + 0.4*
R-Equol 36.4+7.6 50.0 + 10.0* 2.0+0.3*
S-Equol 27.5 + 4.1 33.6 + 7.0 2.0 + 0.4**
*= Significant decrease in anxiety-related parameters (i.e., center area of maze time or time spent in the open arms or number of open arm entries) vs. control values **= Significant decrease in anxiety-related behavior (i.e., number of open arm en-tries ) vs. control values.
n = 8 animals per group [00243] These findings are consistent with those obtained utilizing the 4 die-tary treatments containing different concentrations of isoflavones, and demonstrate that equol is a major factor in regulating anxiety and other neurological states such as mood and depression that have obvious potential for broad health benefits.

[00244] Twenty-nine (29) adults with hypercholesterolemia are fed a diet containing 33 mg of total isoflavones daily for 5 weeks. FIG. 57 shows the ob-served change in BMI for each of the 29 individuals after 5 weeks of strict adher-ence to the diet containing isoflavones. The average reduction in BMI over this period, although small, is nevertheless significant (p=0.01). These results suggest that phytoestrogen-rich diets can influence weight control in humans. The study did not identify the component(s) responsible or the mechanism of weight control.
Average baseline BMI (n=29) is 26.6+-0.8, and the average BMI (n=29) at 5 weeks is 26.2 0.7.

57 `
[00245] While various embodiments of the present invention have been de-scribed in detail, it will be apparent that further modifications and adaptations of the invention will occur to those skilled in the art. It is to be expressly understood that such modifications and adaptations are within the spirit and scope of the pre-sent invention.

[00246] Equol Decreases Depression and Body Weight in Mid-Aged Female Rats:

[00247] Isoflavones have been shown to possess beneficial effects on car-diovascular and hormone-dependent diseases, however, little is known about their influences on brain and behavior, especially behaviors such as depression and de-spair. In this study, mid-aged Long-Evans female rats were examined in this con-text.

[00248] Age-associated morphological and physiological changes in rodents are equivalent to humans using the following age ranges: Mid-aged rats (10 -months of age) are roughly equivalent to humans 45 - 55 years of age, while aged rats (21-23 months of age) are roughly equivalent to humans 65 - 75 years of age (D. Mileusnic et al., Neurobiol. Aging, 20: 19-35, 1999). Additionally, mid-aged rats (at 12 months old) are equivalent in age to postmenopausal women at ap-proximately 55 years of age. The average age of menopause in the United States is approximately 51 years of age (National Institutes of Health, USA).
[00249] All female rats in this study were examined for hormonal patterns of estrus cycling characteristics. All animals in this study were not cycling which is consist with scientific reports using this aging animal model (C.R. Anzalone et al., Biol. Reprod., 64: 1056-1062, 2001).

[00250] The female rats were exposed (from time of conception) to either a phytoestrogen-rich (Phyto-600) or a phytoestrogen-free (Phyto-free) diet.
[00251] At 12 months of age, the animals were analyzed using the Porsolt forced swim test that is a standard preclinical test with applications to humans for investigating the neurobiology of depression and of action of antidepressant agent (R.D. Porsolt, M. Pichon and M Jalfre, Nature 266: 73 0-732, 1977; and M.A.
Geyer & A. Markou, in Psychopharmacology: The Fourth Generation of Pro-gress, NY, Raven Press, pp. 787-798, 1995).
[002521 The test is designed to force rats to swim vigorously. Over time, some of the animals may become immobile which is considered a condition of be-havioral despair. The total time an animal is immobile is an index of despair or depression. Other parameters include: a) total distance traveled, b) total time mo-bile (the opposite of the immobile index), c) number of dives the animal attempts demonstrating escape behaviors and d) the number of defecations or boli excreted during the test which indexes the level of emotionally (C.S. Hall, J. Cornp.
Psych., 18: 385-403, 1934), increased boli numbers during the test would indicate heighten emotional behavior. All of these parameters were recorded in this study and quantified by AnyMaze computer software and the total time of the Porsolt forced swim test was 480 seconds or 8 minutes.
[00253] Thus, animals displaying increased levels of total distance traveled, mobility times and dives along with low numbers for total time immobile and boli excreted would exhibit low levels of despair or depression. Animals exhibiting behaviors opposite to the parameters above would display increased levels of de-spair or depression.
[00254] The animals were tested twice.
[00255] Test 1 compared diet exposure alone, shown as A in FIGS. 58-69 and labeled as "before equol injections."
[00256] In the second stage of testing the Phyto-free fed females were in-jected with equol (@ 5 mg/day or 2.5 mg/kg via subcutaneous administration) for 8 days while the Phyto-600 fed females received dimethylsulfoxide (DMSO) vehi-cle control injections. (DMSO has been used in medicine due to its ability to penetrate deeply through the skin and other membranes without damaging them and ability to carry compounds into the circulation).
[00257] Test 2 assessed depressive behaviors following the equol treatments, shown as B in FIGS. 58-69 and labeled as "after equol injections."

[002581 In the first Porsolt test - labeled A: Phyto-600 fed females dis-played significantly greater a) distance traveled (meters), see FIG. 58 A; b) overall average speed (meters/second), see FIG. 59 A; c) total time mobile (seconds), see FIG. 60 A; and d) number of dives compared to.Phyto-free females, see FIG. 61 A. Conversely, the Phyto-free females displayed significantly greater a) total time immobile (seconds), see FIG. 62 A and b) boli excreted, see FIG. 63 A, compared to Phyto-600 females.
[002591 Following the equol treatments for 8 days, the Phyto-free females displayed values that were similar to those exhibited by the Phyto-600 females for all of the parameters tested above. For example, a) total distance traveled was in-creased and similar to control values, see FIG. 58 B; b) increased average speed was also similar to controls, see FIG. 59 B; c) along with increased total time mo-bile, see FIG. 60 B (the total time mobile significantly increased in the Phyto-free rats after the equol injections compared to the before equol injections levels (see * *)); d) and the number of dives was significantly reduced and comparable to con-trol levels, see FIG. 61 B. Furthermore, the total time immobile was significantly reduced and similar to controls, see FIG. 62 B. Finally, as illustrated in FIG. 63 B, the number of boli was reduced after equol treatments and this was essentially the same to that of control values.
[00260] Also, the average body weight loss (in grams) at the end of the 8 day equol treatments revealed that equol significantly decreased body weights compared to controls, see TABLE 6, representing a 2.6-fold decrease in body weight over controls after the 8 day treatment interval.
[00261] TABLE 6. Body Weight Loss in Mid-Aged Female Long-Evans Rats Treated with Equol Treatment Group Body Weight Loss (grams) Phyto-free plus EQUOL - 31.2( 4.8*) Phyto-600 plus vehicle - 11.7 ( 2.1) *= significant decrease in body weight in equol-treated mid-aged female rats vs.
control values (SEM = standard error of the mean) [00262] These data suggest that exposure to equol, including short term ex-posure, has beneficial antidepressant effects in mid-aged female rats. It is believed that the positive anti-anxiety parameters seen in the Phyto-600 fed animals was due to consuming the enriched isoflavone (soy) diet that in turn would then be converted to equol in these rats. It is well established that rats generate very high levels of equol when fed an enriched soy diet (i.e. the Phyto-600 diet) and that a majority (70 to 90 % of the total) of the circulating isoflavone/isoflavone metabo-lite levels are represented by equol. Notably, when equol was administered (short-term) to mid-aged female rats fed a Phyto-free diet all of the Porsolt test depres-sant parameters (shown in B in FIGS. 58-63) were reversed and were similar to the Phyto-600 group. These data sets are strengthened by the fact that the Phyto-600 and Phyto-free animals served as their own controls, before and after the equol injections. Thus, the results of this study indicate that equol alone ac-counted for all or at least the majority of the positive effects of significantly reduc-irig depressive-like behaviors. As such, the use of equol has great promise in im-proving mood, despair and depressant-related behaviors with potential food, nutri-tional and pharmaceutical applications.

[00263] Equol Decreases Anxiety-Related Behaviors and Body Weight In Adult Male Rats, But Does Not Alter Hormone Sensitive Brain Regions [00264] Hypothalamic structures such as the sexually diinorphic nucleus (SDN) and anterioventroperiventicular nucleus (AVPV) are important regions in-volved in reproductive function/regulatory behaviors that are sensitive to steroid hormonal influences during perinatal and subsequent postnatal development in rats. It was previously shown that SDN and AVPV structures are sensitive to isoflavone influences in adult rats and, in general, many reports have demonstrated that phytoestrogens are neuroprotective (E.D. Lephart et al., J. Steroid Biochem.
Mol. Biol., 85: 299-309, 2003; E. D. Lephart et al., Curr. Neurovas. Res., 1:

464, 2004; and L. Bu & E.D. Lephart, Neurosci. Lett., 385: 153-157, 2005). How-ever, equol, the major isoflavone metabolite in rodents (and many other animal models) has not been examined in brain studies.
[00265] In this study, anxiety-related behaviors were examined along with hormone levels in adult Long-Evans male rats exposed to equol. A known and accepted animal behavioral test for psychiatric disorders in humans, such as ele-vated plus maze (EPM) was used for this purpose (P. Willner, in Behavioral Mod-els in Psychopharmacology: Theoretical, Industrial and Clinical Perspectives, New York, Cambridge Univ. Press, 1991; M.A. Geyer & A. Markou, in Ps,y-chopharmacology: The Fourth Generation of Progress, NY, Raven Press, pp.
787-798, 1995; and R. Hitzemann, Alcohol & Res. Health, 24: 149-158, 2000).
[00266] The EPM test relies on the inherent conflict between exploration of a novel environment and avoidance of its aversive features. Typically, aiiimals spend little time and make few entries into the open arms (new environment) compared to the closed arms of the maze (secure environment). For example, an anti-anxiety agent or drug would increase the number of entries into and total time spent in the open arms of the maze.
[00267] Male rats 50 days of age were given a diet low in phytoestrogens (10 ppm of isoflavones) and remained on this diet until 215 days of age. At days of age the rats were age and body weight matched and then divided into con-trol or equol treatment groups. Starting at 190 days, the male rats received daily subcutaneous (0.1 cc) injections of control vehicle (DMSO) or equol (2.5 mg/Kg) for 25 consecutive days.
[00268] After 15 days on the treatments the animals were tested in the EPM
that was video-taped for 3 minutes and later recorded for the number of entries into- and the percentage of total time spent - on the open arms via AnyMaze computer software. `
[00269] At 215 days of age the animals were sacrificed and body/prostate weights, serum hormone and isoflavone levels were determined along with deter-mining the volume of hypothalamic regions that are sensitive to hormones.

[00270] The significant reductions in prostate weight and 5a-DHT, along with no alteration in serum testosterone, luteinizing hormone (LH) or 17(3-estradiol levels in equol-treated male rats compared to control values, were previ-ously reported in U.S. Publication No. 2005/0245492 A1, contents of which are incorporated by reference in its entirety.
[00271] The brains were processed via standard staining and analyzed via Bioquant for morphometric SDN and AVPV parameters by treatments. The se-rum equol levels in equol-treated animals were equivalent to consuming a phy-toestrogen-rich soy diet.
[00272] As illustrated in FIG. 64, body weights were significantly decreased in the equol-treated males as compared to control animals. In the EPM, equol-treated animals displayed significantly less anxiety-related behaviors compared to control values (i.e., the number of entries into- and time spent on- the open arms of the maze were significantly greater vs. controls), see FIGS. 65 and 66, respec-tively.
[00273] No significant alterations in SDN or AVPV volumetric morphomet-ric parameters were seen with the exposure to equol for 25 consecutive days, see FIG. 67 and TABLE 7.
[00274] These results suggest that equol does not alter hormone-sensitive hypothalamic volumes in rodents during adulthood which is opposite to the con-sumption of soy (phytoestrogens) via dietary routes for an equivalent exposure in-terval previously reported by our laboratory. This further suggests that equol ap-pears may be a safe and nontoxic agent with potential neural applications to ad-dress brain/behavior disorders and men's/women's health issues. Finally, these data suggest that the presence of equol in nutritional food products, dietary sup-plements or pharmaceutical applications would be an effective treatment in weight control and to modulate or reduce anxiety.

[00275] TABLE 7: Morphometric Volumes (expressed in mm3 x 10 "3) of the Hypothalamic Structures- The Sexually Dimorphic Nucleus (SDN) and The Ante-rioventroperiventicular Nucleus (AVPV) of Male Rats Treated with Equol.
Treatment Group SDN Volumes AVPV Volumes Control 53.2+2.9 10.58+2.4 Equol 46.3 3.1 10.59 + 1.6 Regional brain volumes were expressed in mm x 10 ". There were no significant differences in the SDN or AVPV volumes between the treatment groups, n = 4 per group.

[00276] Equol Is More Potent Compared To Genistein In Decreasing Anxi-ety Levels Via Pre- and Early Postnatal Treatments In Male Rat Offspring Tested As Adults [00277] In recent years isoflavones are reported to have beneficial health effects regarding cancers (breast and prostate), cardiovascular disease, osteoporo-sis and symptoms associated with menopause. However, little information is known about the influence of isoflavones on brain and especially behavior such as anxiety. In this study, the long-term effects of genistein and equol (an isoflavone metabolite) were examined for their potential to influence anxiety-related behav-iors in male offspring tested as adults in the EPM.
[002781 Pregnant Long-Evans rats were treated via daily subcutaneous injec-tions from gestational day 14 through 20 and during lactation from postnatal day 2 through 25 with: 1) control-DMSO vehicle or, 2) genistein @ 3 mg/day or 3) equol @ 3 mg/day according to the treatment groups. All animals were fed a phy-toestrogen-low diet tlzroughout these studies (10 ppm isoflavones). There were no significant differences in maternal body weights just before birth among the treat-ment groups. At birth, body weights were slightly (but not significantly) higher in genistein- and equol-treated animals compared to controls. The maternal and male offspring genistein and equol levels (@ 25 days post birth) paralleled that of the treatments administered.
[00279] At approximately 90 days of age the male offspring were tested by treatments (control, genistein and equol) in the EPM that was video-taped for minutes and later recorded for the number of entries into- and the percentage of total time spent - on the open arms, as previously described. As illustrated in FIG.
68, equol-treated males displayed significantly greater entries into the open arms compared to controls. However, the entries into the open arms were not signifi-cantly different between the genistein and control groups. Notably, as seen in FIG. 69, equol-treated males displayed significantly greater time in the open arms compared to genistein-treated animals or controls. Also, genistein-treated males displayed significantly greater open arm time intervals compared to controls.
Equol time intervals were 2-fold greater than genistein values and genistein levels were approximately 9-fold greater than controls.
[00280] These data suggest that: 1) equol is a more potent anti-anxiety agent compared to genistein and 2) the isoflavone metabolite, equol has long-term influ-ences upon anxiety-related behaviors of offspring tested as adults that were treated prenatally. The findings of this experiment validate the positive effects of equol seen in Example 17 described above for reducing depressive-related behaviors that are presumably due to circulating equol levels and not genistein. The results of this study have applications to food, nutritional and pharmaceutical modulation of anxiety by various routes of administration.

[00281] It is therefore intended that the foregoing detailed description be re-garded as illustrative rather than limiting, and that it be understood that it is the following claims, including all equivalents, that are intended to define the spirit and scope of this invention.

Claims (19)

1. A method of preventing or ameliorating a neuropsychiatric or neurode-generative disease or disorder in a subject, comprising:
administering a composition comprising equol in an amount suffi-cient to prevent or ameliorate the neuropsychiatric or neurodegenerative disease or disorder.

2. The method of claim 1, wherein the equol is a racemic mixture of R-equol and S-equol.

3. The method of claim 1, wherein the equol is enantiomerically enriched with R-equol.

4. The method of claim 1, wherein the equol is enantiomerically enriched with S-equol.

5. The method of claim 1, wherein the neuropsychiatric or neurodegenera-tive disease or disorder is depression, anxiety, bipolar disorder, obsessive-compulsive disorder, hyperactive disorder, panic disorder, weight gain, obesity, or bulimia.

6. The method of claim 1, wherein the neuropsychiatric or neurodegenera-tive disease or disorder is Alzheimer's disease or Parkinson's disease.

7. The method of claim 1, wherein the composition further comprises an excipient.

8. The method of claim 1, wherein the composition is in an oral formula-tion selected from the group consisting of a tablet, capsule, powder, trouche, buc-cal tablet, and sublingual tablet.

9. The method of claim 1, wherein the composition is an oral formulation comprising at least about 0.25 mg of equol.

10. The method of claim 1, wherein the composition is selected from the group consisting of a food product and a beverage.

11. The method of claim 1, wherein the composition is a delayed or a sus-tained release formulation.

12. The method of claim 1, wherein the neuropsychiatric or neurodegenera-tive diseases or disorder is a peri- or postmenopausal symptom.

13. The method of claim 12, wherein the peri- or postmenopausal symptom is depression, anxiety, fatigue, weight gain, obesity, and polycystic ovarian dis-ease.

14. The method of claim 1, wherein the equol is in an amount sufficient to bind free 5a-dihydrotestosterone and inhibit its binding with androgen receptors.

15. The method of claim 1, wherein the equol is in an amount sufficient to bind free 5a-dihydrotestosterone and inhibit its binding with androgen receptors, and sufficient to bind estrogen receptor subtypes.

16. A method of reducing obesity in a subject, comprising administering a composition comprising a therapeutically effective amount of equol.

17. A method of reducing depression in a subject, comprising administering a composition comprising a therapeutically effective amount of equol.

18. A method of reducing anxiety in a subject, comprising administering a composition comprising a therapeutically effective amount of equol.

19. A method of providing a personalized treatment of a neuropsychiatric or neurodegenerative disease or disorder in a subject, comprising:
assessing a condition of the emotional, mental or endocrine health of the patient;
assessing an equol-producer status of a patient;
determining an optimally beneficial course of treatment, selected from the group consisting of a) mode of administration, b) a dose amount, and c) a dose interval.