CA2653038A1 - Un systeme de micropipetage pour le dosage de matieres biologiques ou de macromolecules - Google Patents
Un systeme de micropipetage pour le dosage de matieres biologiques ou de macromolecules Download PDFInfo
- Publication number
- CA2653038A1 CA2653038A1 CA 2653038 CA2653038A CA2653038A1 CA 2653038 A1 CA2653038 A1 CA 2653038A1 CA 2653038 CA2653038 CA 2653038 CA 2653038 A CA2653038 A CA 2653038A CA 2653038 A1 CA2653038 A1 CA 2653038A1
- Authority
- CA
- Canada
- Prior art keywords
- layer
- gel
- microfluidic device
- microchannels
- membrane
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 229920002521 macromolecule Polymers 0.000 title claims description 5
- 239000012620 biological material Substances 0.000 title description 4
- 239000012528 membrane Substances 0.000 claims abstract description 39
- 238000013537 high throughput screening Methods 0.000 claims abstract description 21
- 239000011148 porous material Substances 0.000 claims abstract description 15
- 239000000499 gel Substances 0.000 claims description 54
- 235000013870 dimethyl polysiloxane Nutrition 0.000 claims description 17
- 239000004205 dimethyl polysiloxane Substances 0.000 claims description 17
- 238000000034 method Methods 0.000 claims description 13
- 238000005086 pumping Methods 0.000 claims description 13
- 238000012360 testing method Methods 0.000 claims description 7
- 239000011543 agarose gel Substances 0.000 claims description 5
- 229920001971 elastomer Polymers 0.000 claims description 5
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- 230000010261 cell growth Effects 0.000 claims description 4
- 238000004519 manufacturing process Methods 0.000 claims description 4
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- 239000000017 hydrogel Substances 0.000 claims description 2
- 239000007788 liquid Substances 0.000 claims description 2
- 239000000126 substance Substances 0.000 claims 4
- CXQXSVUQTKDNFP-UHFFFAOYSA-N octamethyltrisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)O[Si](C)(C)C CXQXSVUQTKDNFP-UHFFFAOYSA-N 0.000 claims 2
- 238000004987 plasma desorption mass spectroscopy Methods 0.000 claims 2
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 claims 2
- 238000000151 deposition Methods 0.000 claims 1
- 239000002184 metal Substances 0.000 claims 1
- 229920002401 polyacrylamide Polymers 0.000 claims 1
- 229940000406 drug candidate Drugs 0.000 abstract description 10
- 238000000423 cell based assay Methods 0.000 abstract description 3
- 239000010410 layer Substances 0.000 description 38
- 229940079593 drug Drugs 0.000 description 26
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- 238000009792 diffusion process Methods 0.000 description 12
- 238000004113 cell culture Methods 0.000 description 9
- GLNADSQYFUSGOU-GPTZEZBUSA-J Trypan blue Chemical compound [Na+].[Na+].[Na+].[Na+].C1=C(S([O-])(=O)=O)C=C2C=C(S([O-])(=O)=O)C(/N=N/C3=CC=C(C=C3C)C=3C=C(C(=CC=3)\N=N\C=3C(=CC4=CC(=CC(N)=C4C=3O)S([O-])(=O)=O)S([O-])(=O)=O)C)=C(O)C2=C1N GLNADSQYFUSGOU-GPTZEZBUSA-J 0.000 description 8
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- RBTBFTRPCNLSDE-UHFFFAOYSA-N 3,7-bis(dimethylamino)phenothiazin-5-ium Chemical compound C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 RBTBFTRPCNLSDE-UHFFFAOYSA-N 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 4
- 229960000907 methylthioninium chloride Drugs 0.000 description 4
- 238000013459 approach Methods 0.000 description 3
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- 108020004635 Complementary DNA Proteins 0.000 description 1
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- 150000002894 organic compounds Chemical class 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 238000004094 preconcentration Methods 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000003252 repetitive effect Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
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Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US99612107P | 2007-11-01 | 2007-11-01 | |
| US60/996121 | 2007-11-01 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2653038A1 true CA2653038A1 (fr) | 2009-05-01 |
Family
ID=40589943
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA 2653038 Abandoned CA2653038A1 (fr) | 2007-11-01 | 2008-11-03 | Un systeme de micropipetage pour le dosage de matieres biologiques ou de macromolecules |
Country Status (1)
| Country | Link |
|---|---|
| CA (1) | CA2653038A1 (fr) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2600066A (en) * | 2015-12-04 | 2022-04-20 | Emulate Inc | Open-top microfluidic device with structural anchors |
| US11878301B2 (en) | 2021-06-07 | 2024-01-23 | Credo Diagnostics Biomedical Pte. Ltd. | Analysis cartridge |
-
2008
- 2008-11-03 CA CA 2653038 patent/CA2653038A1/fr not_active Abandoned
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2600066A (en) * | 2015-12-04 | 2022-04-20 | Emulate Inc | Open-top microfluidic device with structural anchors |
| GB2600066B (en) * | 2015-12-04 | 2022-11-02 | Emulate Inc | Open-Top Microfluidic Device With Structural Anchors |
| US11878301B2 (en) | 2021-06-07 | 2024-01-23 | Credo Diagnostics Biomedical Pte. Ltd. | Analysis cartridge |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Dead |
Effective date: 20141104 |