CA2617830A1 - Direct molecular cloning of a modified eukaryotic cytoplasmic dna virus genome - Google Patents
Direct molecular cloning of a modified eukaryotic cytoplasmic dna virus genome Download PDFInfo
- Publication number
- CA2617830A1 CA2617830A1 CA002617830A CA2617830A CA2617830A1 CA 2617830 A1 CA2617830 A1 CA 2617830A1 CA 002617830 A CA002617830 A CA 002617830A CA 2617830 A CA2617830 A CA 2617830A CA 2617830 A1 CA2617830 A1 CA 2617830A1
- Authority
- CA
- Canada
- Prior art keywords
- molecular cloning
- chordopoxvirus
- plasmid
- modified
- virus genome
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention provides a plasmid for producing a modified chordopoxvirus, wherein the plasmid comprises a segment of a chordopoxvirus genome that comprises a thymidine kinase gene of said chordopoxvirus, wherein said thymidine kinase gene has been modified to prevent expression of active thymidine kinase, wherein said plasmid is as shown in Figure 4.2 and is selected from the group of plasmids: pHindJ-2 comprising the sequence of SEQ. ID. NO.
4, and pHindJ-3 comprising the sequence of SEQ. ID. NO. 5.
4, and pHindJ-3 comprising the sequence of SEQ. ID. NO. 5.
Claims (2)
1. A plasmid for producing a modified chordopoxvirus, wherein the plasmid comprises a segment of a chordopoxvirus genome that comprises a thymidine kinase gene of said chordopoxvirus, wherein said thymidine kinase gene has been modified to prevent expression of active thymidine kinase, wherein said plasmid is as shown in Figure 4.2 and is selected from the group of plasmids: pHindJ-2 comprising the sequence of SEQ. ID. NO. 4, and pHindJ-3 comprising the sequence of SEQ. ID. NO. 5.
2. Use of a plasmid as defined in Claim 1 for producing a modified chordopoxvirus by direct molecular cloning.
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US07/750,080 US5445953A (en) | 1991-08-26 | 1991-08-26 | Direct molecular cloning of a modified poxvirus genome |
| US07/750,080 | 1991-08-26 | ||
| US91473892A | 1992-07-20 | 1992-07-20 | |
| US07/914,738 | 1992-07-20 | ||
| CA002558864A CA2558864C (en) | 1991-08-26 | 1992-08-25 | Direct molecular cloning of a modified eukaryotic cytoplasmic dna virus genome |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002558864A Division CA2558864C (en) | 1991-08-26 | 1992-08-25 | Direct molecular cloning of a modified eukaryotic cytoplasmic dna virus genome |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2617830A1 true CA2617830A1 (en) | 1993-02-27 |
| CA2617830C CA2617830C (en) | 2010-04-06 |
Family
ID=39103181
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2617830A Expired - Fee Related CA2617830C (en) | 1991-08-26 | 1992-08-25 | Direct molecular cloning of a modified eukaryotic cytoplasmic dna virus genome |
| CA002616717A Abandoned CA2616717A1 (en) | 1991-08-26 | 1992-08-25 | Direct molecular cloning of a modified eukaryotic cytoplasmic dna virus genome |
| CA002616698A Expired - Fee Related CA2616698C (en) | 1991-08-26 | 1992-08-25 | Direct molecular cloning of a modified eukaryotic cytoplasmic dna virus genome |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002616717A Abandoned CA2616717A1 (en) | 1991-08-26 | 1992-08-25 | Direct molecular cloning of a modified eukaryotic cytoplasmic dna virus genome |
| CA002616698A Expired - Fee Related CA2616698C (en) | 1991-08-26 | 1992-08-25 | Direct molecular cloning of a modified eukaryotic cytoplasmic dna virus genome |
Country Status (1)
| Country | Link |
|---|---|
| CA (3) | CA2617830C (en) |
-
1992
- 1992-08-25 CA CA2617830A patent/CA2617830C/en not_active Expired - Fee Related
- 1992-08-25 CA CA002616717A patent/CA2616717A1/en not_active Abandoned
- 1992-08-25 CA CA002616698A patent/CA2616698C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CA2616698C (en) | 2009-03-17 |
| CA2616717A1 (en) | 1993-02-27 |
| CA2617830C (en) | 2010-04-06 |
| CA2616698A1 (en) | 1993-02-27 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKLA | Lapsed |