CA2590286A1 - Genes coding for flavone synthases - Google Patents
Genes coding for flavone synthases Download PDFInfo
- Publication number
- CA2590286A1 CA2590286A1 CA002590286A CA2590286A CA2590286A1 CA 2590286 A1 CA2590286 A1 CA 2590286A1 CA 002590286 A CA002590286 A CA 002590286A CA 2590286 A CA2590286 A CA 2590286A CA 2590286 A1 CA2590286 A1 CA 2590286A1
- Authority
- CA
- Canada
- Prior art keywords
- plant
- gene
- host
- introducing
- vector
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
Abstract
A DNA encoding an enzyme capable of converting flavanone directly into flavone which is obtained from, for example, antirrhinum and torenia, and utilization thereof. The amino acid sequences of this DNA and the enzyme encoded thereby are represented by, for example, SEQ ID NOS: 1, 2, 3 and 4. Transfer of this gene into a plant makes it possible to, for example, change the flower color of the plant.
Claims (18)
1. A gene which encodes a protein having the amino acid sequence listed as SEQ ID NO: 4.
2. A gene which encodes a protein having an amino acid sequence which has at least 75% identity with the amino acid sequence listed as SEQ ID NO: 4, wherein the protein has an activity of synthesizing flavones from flavanones.
3. A gene according to claim 1 or 2, which hybridizes to the complement of the nucleotide sequence listed as SEQ ID NO: 3 of the Sequence Listing under conditions of 5 × SSC, 50°C, and which encodes a protein possessing activity of synthesizing flavones from flavanones.
4. A vector comprising a gene according to any one of claims 1 to 3.
5. A host cell transformed by a vector according to claim 4.
6. A protein encoded by a gene according to any one of claims 1 to 3.
7. A method of producing a protein with flavone-synthesizing activity, which is characterized by culturing or growing a host according to claim 5 and recovering said protein from said host.
8. A plant cell into which a gene according to any one of claims 1 to 3 has been introduced, or progeny cell of said plant cell maintaining the same gene as introduced.
9. A method of altering a composition of flavonoids or amounts thereof, in a plant host comprising introducing a vector as defined in claim 4 into a plant host, culturing and growing said plant host and recovering flavones, wherein said flavones are included in a composition of flavonoids.
10. A method of altering the amount of a flavone in a plant host comprising introducing a vector as defined in claim 4 into a plant host, culturing and growing said plant host and recovering flavones, wherein said flavones are included in a composition of flavonoids.
11. A method of altering the color of a flower of a plant host comprising introducing a vector as defined in claim 4 into a plant host, culturing and growing said plant host.
12. A method of bluing the color of a flower of a plant host comprising introducing a vector as defined in claim 4 into a plant host, culturing and growing said plant host.
13. A method of reddening the color of a flower of a plant host comprising introducing a vector as defined in claim 4 into a plant host, culturing and growing said plant host.
14. Use of a gene as defined in any one of claims 1 to 3 for altering a composition of flavonoids and its amount in a plant by introducing said gene into said plant and expressing the gene in the plant.
15. Use of a gene as defined in any one of claims 1 to 3 for altering the amount of a flavone in a plant by introducing said gene into said plant and expressing the gene in the plant.
16. Use of a gene as defined in any one of claims 1 to 3 for altering the color of a flower of a plant by introducing said gene into said plant and expressing the gene in the plant.
17. Use of a gene as defined in any one of claims 1 to 3 for bluing the color of a flower of a plant by introducing said gene into said plant and expressing the gene in the plant.
18. Use of a gene as defined in any one of claims 1 to 3 for reddening the color of a flower of a plant by introducing said gene into said plant and expressing the gene in the plant.
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP11/22427 | 1999-01-29 | ||
| JP2242799 | 1999-01-29 | ||
| JP11/205229 | 1999-07-19 | ||
| JP20522999A JP4368005B2 (en) | 1999-01-29 | 1999-07-19 | Gene encoding flavone synthase |
| CA2324505A CA2324505C (en) | 1999-01-29 | 2000-01-28 | Genes coding for flavone synthases |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2324505A Division CA2324505C (en) | 1999-01-29 | 2000-01-28 | Genes coding for flavone synthases |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2590286A1 true CA2590286A1 (en) | 2000-08-03 |
| CA2590286C CA2590286C (en) | 2010-12-14 |
Family
ID=38445924
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2590286A Expired - Lifetime CA2590286C (en) | 1999-01-29 | 2000-01-28 | Genes coding for flavone synthases |
| CA2591790A Expired - Fee Related CA2591790C (en) | 1999-01-29 | 2000-01-28 | Genes coding for flavone synthases |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2591790A Expired - Fee Related CA2591790C (en) | 1999-01-29 | 2000-01-28 | Genes coding for flavone synthases |
Country Status (1)
| Country | Link |
|---|---|
| CA (2) | CA2590286C (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113980984A (en) * | 2021-11-11 | 2022-01-28 | 中国医学科学院药用植物研究所 | Gene SmLAC1 and application thereof in regulating and controlling procyanidine synthesis |
-
2000
- 2000-01-28 CA CA2590286A patent/CA2590286C/en not_active Expired - Lifetime
- 2000-01-28 CA CA2591790A patent/CA2591790C/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113980984A (en) * | 2021-11-11 | 2022-01-28 | 中国医学科学院药用植物研究所 | Gene SmLAC1 and application thereof in regulating and controlling procyanidine synthesis |
| CN113980984B (en) * | 2021-11-11 | 2024-04-26 | 中国医学科学院药用植物研究所 | Gene SmLAC1 and application thereof in regulating procyanidine synthesis |
Also Published As
| Publication number | Publication date |
|---|---|
| CA2590286C (en) | 2010-12-14 |
| CA2591790A1 (en) | 2000-08-03 |
| CA2591790C (en) | 2010-12-21 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKEX | Expiry |
Effective date: 20200128 |